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1.
Plants act as an important link between atmosphere and soil: CO2 is transformed into carbohydrates by photosynthesis. These assimilates are distributed within the plant and translocated via roots into the rhizosphere and soil microorganisms. In this study, 3 year old European beech trees (Fagus sylvatica L.) were exposed after the chilling period to an enriched 13C–CO2 atmosphere (δ13C = 60‰ – 80‰) at the time point when leaves development started. Temporal dynamics of assimilated carbon distribution in different plant parts, as well as into dissolved organic carbon and microbial communities in the rhizosphere and bulk soil have been investigated for a 20 days period. Photosynthetically fixed carbon could be traced into plant tissue, dissolved organic carbon and total microbial biomass, where it was utilized by different microbial communities. Due to carbon allocation into the rhizosphere, nutrient stress decreased; exudates were preferentially used by Gram-negative bacteria and (mycorrhizal) fungi, resulting in an enhanced growth. Other microorganisms, like Gram-positive bacteria and mainly micro eucaryotes benefited from the exudates via food web development. Overall our results indicate a fast turnover of exudates and the development of initial food web structures. Additionally a transport of assimilated carbon into bulk soil by (mycrorhizal) fungi was observed.  相似文献   

2.
A greenhouse experiment was conducted by growing oats (Avenasativa L.) in a continuously 13CO2 labeled atmosphere. The allocation of 13C-labeled photosynthates in plants, microbial biomass in rhizosphere and root-free soil, pools of soil organic C, and CO2 emissions were examined over the plant's life cycle. To isolate rhizosphere from root-free soil, plant seedlings were placed into bags made of nylon monofilament screen tissue (16 μm mesh) filled with soil. Two peaks of 13C in rhizosphere pools of microbial biomass and dissolved organic carbon (DOC), as well as in CO2 emissions at the earing and ripeness stages were revealed. These 13C maxima corresponded to: (i) the end of rapid root growth and (ii) beginning of root decomposition, respectively. The δ13C values of microbial biomass were higher than those of DOC and of soil organic matter (SOM). The microbial biomass C accounted for up to 56 and 39% of 13C recovered in the rhizosphere and root-free soil, respectively. Between 4 and 28% of 13C assimilated was recovered in the root-free soil. Depending on the phenological stage, the contribution of root-derived C to total CO2 emission from soil varied from 61 to 92% of total CO2 evolved, including 4-23% attributed to rhizomicrobial respiration. While 81-91% of C substrates used for microbial growth in the root-free soil and rhizosphere came from SOM, the remaining 9-19% of C substrates utilized by the microbial biomass was attributable to rhizodeposition. The use of continuous isotopic labelling and physical separation of root-free and rhizosphere soil, combined with natural 13C abundance were effective in gaining new insight on soil and rhizosphere C-cycling.  相似文献   

3.
Although soil-dwelling Collembola can influence plant growth and nutrient cycling, their specific role in soil food webs is poorly understood. Soil-free microcosm studies suggest that Collembola are primarily fungivores where they feed preferentially on saprophytic fungi (SF) over other fungal types. We directly assessed collembolan consumption of arbuscular mycorrhizal fungi (AMF) and SF using plant-soil mesocosms and natural abundance stable carbon isotope techniques. Mycorrhizal Andropogon gerardii (C4 grass) seedlings were placed in pots containing Collembola and soil from a C3 plant dominated site, while mycorrhizal Pascopyrum smithii (C3 grass) seedlings were placed in pots with Collembola and soil collected at a C4 plant dominated site. After 6 weeks, collembolans assimilated carbon derived from C3 and C4 sources in both A. gerardii and P. smithii treatments. Comparing Collembola isotope values in AMF vs. AMF-suppressed treatments, our data show that both AMF and SF were consumed in these experimental soil environments.  相似文献   

4.
Photosynthesis of higher plants drives carbon (C) allocation below-ground and controls the supply of assimilates to roots and to rhizosphere microorganisms. To investigate the effect of limited photosynthesis on C allocation, redistribution and reutilization in plant and soil microorganisms, perennial grass Lolium perenne and legume Medicago sativa were clipped or shaded. Plants were labelled with three 14C pulses to trace allocation and reutilization of C assimilated before clipping or shading. Five days after the last 14C pulse, plants were clipped or shaded and the total CO2 and 14CO2 efflux from the soil was measured. 14C in above- and below-ground plant biomass and bulk soil, rhizosphere soil and microorganisms was determined 10 days after clipping or shading.After clipping, 2% of the total assimilated 14C originating mainly from root reserves were detected in the newly grown shoots. This corresponded to a translocation of 5 and 8% of total 14C from reserve organs to new shoots of L. perenne and M. sativa, respectively. The total CO2 efflux from soil decreased after shading of both plant species, whereas after clipping, this was only true for L. perenne. The 14CO2 efflux from soil did not change after clipping of both species. An increased 14CO2 efflux from soil under shading for both plants indicated that lower assimilation was compensated by higher utilization of the reserve C for root and rhizomicrobial respiration.We conclude that C stored in roots is an important factor for plant recovery after limiting photosynthesis. This stored C is important for shoot regrowth after clipping, whereas after shading, it is utilized mainly for maintenance of root respiration. Based on these results as well as on a review of several studies on C reutilization for regrowth after clipping, we conclude that because of the high energy demand for nitrogen fixation, legumes use a higher portion (9–10%) of stored C for regrowth compared to grasses (5–7%). The effects of limited photosynthesis were of minor importance for the exudation of the reserve C and thus, have no effect on the uptake of this C by microorganisms.  相似文献   

5.
The mycorrhizal, rhizosphere and basal components of soil respiration were partitioned in a barley field experiment with the main objective of determining the controlling effects of photosynthetic activity and temperature on soil respiration sources. Micro-pore meshes were used to create both root and mycorrhiza-free soil cores over which collars for soil respiration measurements were inserted. Differences between mesh treatments were used to determine the contribution of each component. With a focus on the growing season, we analyzed the response of respiration sources to photosynthesis, temperature and moisture, as well as changes in microbial biomass, mineral nitrogen and carbon-nitrogen ratios responding to treatment and time of year. Results gave clear differences between sources in their response to both temperature and photosynthetic activity and showed that several processes are involved in determining respiration rates as well as apparent temperature relations. In particular, the respiration of arbuscular mycorrhizal hyphae was seen to be a significant amount of root derived carbon respiration (25.3%) and consequently of total assimilated carbon (4.8%). This source showed a stronger response to photosynthetic activity than the rhizosphere component (r2=0.79, p<0.001 and r2=0.324, p=0.53, respectively). Q10 values—the increase in respiration rates with a 10 °C increase in temperature—changed seasonally and showed temperature relations being dependent on the presence of mycorrhizal and rhizosphere respiration sources, as well as on plant development. Respiration from mycorrhizal hyphae and the rhizosphere showed no response (r2=0, p<0.99) or low response (r2=0.14, p<0.01) to temperature, respectively. We conclude that the potential importance and controls of mycorrhizal fungi respiration in croplands are comparable to those observed in other ecosystems, and that temperature response curves should be carefully interpreted given that substrate availability and plant dynamics strongly regulate respiration rates in ecosystems.  相似文献   

6.
A theoretical approach to the partitioning of carbon dioxide (CO2) efflux from soil with a C3 vegetation history planted with maize (Zea mays), a C4 plant, into three sources, root respiration (RR), rhizomicrobial respiration (RMR), and microbial soil organic matter (SOM) decomposition (SOMD), was examined. The δ13C values of SOM, roots, microbial biomass, and total CO2 efflux were measured during a 40-day growing period. A three-source isotopic mass balance based on the measured δ13C values and on assumptions made in other studies showed that RR, RMR, and SOMD amounted to 91%, 4%, and 5%, respectively. Two assumptions were thoroughly examined in a sensitivity analysis: the absence of 13C fractionation and the conformity of δ13C of microbial CO2 and that of microbial biomass. This approach strongly overestimated RR and underestimated RMR and microbial SOMD. CO2 efflux from unplanted soil was enriched in 13C by 2.0‰ compared to microbial biomass. The consideration of this 13C fractionation in the mass balance equation changed the proportions of RR and RMR by only 4% and did not affect SOMD. A calculated δ13C value of microbial CO2 by a mass balance equation including active and inactive parts of microbial biomass was used to adjust a hypothetical below-ground CO2 partitioning to the measured and literature data. The active microbial biomass in the rhizosphere amounted to 37% to achieve an appropriate ratio between RR and RMR compared to measured data. Therefore, the three-source partitioning approach failed due to a low active portion of microbial biomass, which is the main microbial CO2 source controlling the δ13C value of total microbial biomass. Since fumigation-extraction reflects total microbial biomass, its δ13C value was unsuitable to predict δ13C of released microbial CO2 after a C3-C4 vegetation change. The second adjustment to the CO2 partitioning results in the literature showed that at least 71% of the active microbial biomass utilizing maize rhizodeposits would be necessary to achieve that proportion between RR and RMR observed by other approaches based on 14C labelling. The method for partitioning total below-ground CO2 efflux into three sources using a natural 13C labelling technique failed due to the small proportion of active microbial biomass in the rhizosphere. This small active fraction led to a discrepancy between δ13C values of microbial biomass and of microbially respired CO2.  相似文献   

7.
The direction of carbon (C) allocation in mycorrhizal mycelia is of fundamental importance to coexistence of individual plants. We therefore investigated the transfer of C from established plants to plant seedlings through fungal mycelia. C allocation by the arbuscular mycorrhizal (AM) fungus Glomus intraradices, from ‘donor’ plants to mycelia in soil and two different species of introduced ‘receiver’ seedlings, was investigated in a pot experiment using 13C labelling and fatty acid analysis. After 13CO2 application to the shoots of Trifolium subterraneum or Plantago lanceolata, used as donor plants, T. subterraneum and P. lanceolata receiver seedlings were introduced. Samples were collected 4-20 days after 13CO2 application and analysed regarding 13C and the fatty acid 16:1ω5, the signature of AM fungi. 13C transfer from T. subterraneum donor plants was demonstrated by 13C enrichment of the roots of the receiver seedlings, but not from the P. lanceolata donor plants. 13C allocation to the neutral lipid fatty acid 16:1ω5 was only 1 ng in each receiver seedling, but 2 μg of the fatty acid in whole soil. The results indicate that C allocation through mycelial networks is influenced by the donor plant species, but is not directed towards receiver seedlings to any higher degree than towards other directions. The importance of the extraradical AM fungal mycelium as a C sink was demonstrated.  相似文献   

8.
In the symbiosis between nodulated legume roots and arbuscular mycorrhizal (AM) fungi, the C and N economy can be influenced by the source of N-supply from either AM-derived NH4+ uptake or nodule-derived biological nitrogen fixation (BNF). This relationship was investigated in terms of NH4+ supply and BNF by the two symbionts. Nodulated Phaseolus vulgaris seedlings with and without AM, were hydroponically grown with either 0 N or 1 mM NH4+ supply. Plants were harvested at 30 days after emergence and measurements were taken for biomass, N2 fixation, photosynthesis, CO2 and O2 root respiration, calculated C and N economy. AM roots had higher NH4+ uptake and this was associated with the suppression of BNF and nodule growth. The higher NH4+ uptake in AM roots occurred with lower root maintenance respiration, compared to when N was derived from BNF. There was also an increase in the below-ground sink strength of NH4+ fed AM roots compared to NH4+ fed non-AM roots, as evidenced by the increases in root CO2 and O2 respiration and photosynthetic stimulation. These results indicate that although the AM root had higher total below-ground respiratory costs during NH4+ nutrition, there were lower respiratory C costs associated with N derived from AM symbionts in comparison to N from BNF.  相似文献   

9.
Carbon assimilation and allocation were studied in a tundra ecosystem in northern Scandinavia. Seasonal variation in the below-ground carbon allocation to dissolved organic carbon (DOC), coarse-, fine-, and hair roots was investigated using in situ 14C pulse-labelling, adding 2-3 MBq 14CO2 dm−2 to the above-ground vegetation. Combining the allocation data with regression models of the seasonal carbon flux made it possible to estimate a temporally explicit ecosystem carbon allocation budget.The ecosystem was a net source of CO2, losing on average 0.97 g C m−2 d−1 to the atmosphere, with little variation through the season. There was, however, significant temporal variation in partitioning of recently assimilated carbon. Allocation to below-ground compartments over 32 days following labelling increased from 18% in June to 55% in September. Above-ground allocation showed the opposite trend. Hair roots and DOC were strong sinks in the autumn. Transport of newly assimilated carbon occurred rapidly throughout the season, 14C appearing in all sampled pools within 4 h of labelling.The seasonal variation in carbon partitioning observed in this study has implications for the residence time of assimilated carbon in the ecosystem. A relatively greater allocation to rapidly decomposing pools, such as hair roots and DOC, would tend to reduce incorporation into woody tissue, increasing the overall rate of carbon cycling and decreasing ecosystem storage. The results of this study will be of value for building and validating mechanistic models of ecosystem carbon flow in tundra and subarctic ecosystems.  相似文献   

10.
This study investigated the effects of inoculation with three individual ectomycorrhizal (ECM) fungal species on soil microbial biomass carbon and indigenous bacterial community functional diversity in the rhizosphere of Chinese pine (Pinus tabulaeformis Carr.) seedlings under field experimental conditions. The results showed that ECM fungal inoculation significantly increased the ectomycorrhizal colonization compared with non-inoculated seedlings. ECM fungal inoculations have higher soil microbial biomass carbon than that of control, ranging from 49.6 μg C g?1 dry soil in control to 134.02 μg C g?1 dry soil in treatment inoculated with Boletus luridus Schaeff ex Fr. Multivariate analyses (PCA) of BIOLOG data revealed that the application of ECM fungi significantly influenced bacterial functional diversity in the rhizosphere of P. tabulaeformis seedlings. The highest average well-color development (AWCD) and functional diversity indices were also observed in treatment inoculated with B. luridus. A wider range of sole carbon sources were utilized by the bacterial community in the rhizosphere of inoculated seedlings. The data gathered from this study provides important information for utilization of ECM fungi in forest restoration project in the Northwestern China. The present study will also significantly broaden our understanding of practical importance in the application of ECM fungal inoculum to promote soil microbial community diversity of soil.  相似文献   

11.
The effects of enriched CO2 atmosphere on partitioning of recently assimilated carbon were investigated in a plant-soil-microorganism system in which Lolium perenne seedlings were planted into cores inserted into the resident soil within a sward that had been treated with elevated CO2 for 9 consecutive years, under two N fertilisation levels (Swiss FACE experiment). The planted cores were excavated from the ambient (35 Pa pCO2) and enriched (60 Pa pCO2) rings at two dates, in spring and autumn, during the growing season. The cores were brought back to the laboratory for 14C labelling of shoots in order to trace the transfer of recently assimilated C both within the plant and to the soil and microbial biomass. At the spring sampling, high N supply stimulated shoot and total dry matter production. Consistently, high N enhanced the allocation of recently fixed C to shoots, and reduced it to belowground compartments. Elevated CO2 had no consequences for DM or the pattern of C allocation. At the autumn sampling, at high N plot, yield of L. perenne was stimulated by elevated CO2. Consistently, 14C was preferentially allocated aboveground and, consequently belowground recent C allocation was depressed and rhizodeposition reduced. At both experimental periods, total soil C content was similar in all treatments, providing no evidence for soil carbon sequestration in the Swiss Free Air CO2 Enrichment experiment (FACE) after 9 years of enrichment. Recently assimilated C and soil C were mineralised faster in soils from enriched rings, suggesting a CO2-induced shift in the microbial biomass characteristics (structure, diversity, activity) and/or in the quality of the root-released organic compounds.  相似文献   

12.
In this study we quantify the partitioning of recent assimilates to above- and below-ground carbon (C) pools in two subarctic mire ecosystems - wet minerotrophic and semiwet ombrotrophic mire - using in situ 14C pulse-labelling. Ecosystem C partitioning to rhizomes, coarse roots, fine roots, dissolved organic carbon (DOC) and microbes were quantified twice  during the growing season at three different soil depths. Finally the 14C-partitioning data from this and a previous study were combined to estimate the overall C partitioning of the three main vegetation types of a Scandinavian subarctic mire in early and late summer.The semiwet ombrotrophic ecosystem hosted a much larger root biomass on an area basis compared to the wet minerotrophic ecosystem which might be due to differences in the soil nutrient level. Microbial C was found to be the largest C-pool in both ecosystems. Ecosystem 14C partitioning was poorly related to plant biomass for the semiwet and the wet ecosystem. Overall a higher partitioning of recent assimilates to below-ground compartments was apparent in August-September compared to June-July, while the opposite was found for the above-ground C-pools. In the semiwet ecosystem twice as much 14C was found in DOC compared to the wet ecosystem, where root density, litter and above-ground biomass were important controls of the 14C-recovery in DOC. Plant-derived DOC was estimated to be 15.4 versus 12.9 mg C m−2 d−1 in the semiwet and wet ecosystem, respectively.Graminoid dominated and dwarf shrub dominated vegetation types of the subarctic mire Stordalen differ with respect to the relative amount of recently assimilated C partitioned to C-pools with “slow” versus “fast” decomposition rate. The capacity for sequestration of recently fixed C within “slow” C-pools might affect the ecosystem C balance (NEE) and C-storage. The potential for vegetation changes might therefore be an important factor to consider in studies of response of ecosystem C-dynamics to global change factors in subarctic mires.  相似文献   

13.
To understand the implications of atmospheric nitrogen deposition on carbon turnover in peatlands, we conducted a 13C pulse labeling experiment on Calluna vulgaris and Eriophorum vaginatum already receiving long-term (5 years) amendments of 56 kg N ha−1 y−1 as ammonium or nitrate. We examined shoot tissue retention, net ecosystem respiration returns of the 13C pulse, and soil porewater DOC content under the two species. 13C fixation in Eriophorum leaves was enhanced with nitrogen addition and doubled with nitrate supply. This newly fixed C appeared to be relocated below-ground faster with nitrogen fertilization as respiration returns were unaffected by N inputs. By contrast, increases in 13C fixation were not observed in Calluna. Instead, net ecosystem respiration rates over Calluna increased with N fertilization. There was no significant label incorporation into DOC, suggesting a conservative strategy of peatland vegetation regarding allocation of C through root exudation. Greater concentrations of total DOC were identified with nitrate addition in Calluna. Given the long-term nature of the experiment and the high N inputs, the overall impacts of nitrogen amendments on the fate of recently synthesized C in Eriophorum and Calluna in this ombrotrophic peatland were surprisingly more moderate than originally hypothesized. This may be due to N being effectively retained within the bryophyte layer, thus limiting, and delaying the onset of, below-ground effects.  相似文献   

14.
The dynamics of C partitioning with Lolium perenne and its associated rhizosphere was investigated in plant-soil microcosms using 14C pulse-chase labelling. The 14CO2 pulse was introduced into the shoot chamber and the plants allowed to assimilate the label for a fixed period. The microcosm design facilitated independent monitoring of shoot and root/soil respiration during the chase period. Partitioning between above- and below-ground pools was determined between 30 min and 168 h after the pulse, and the distribution was found to vary with the length of the chase period. Initially (30 min after the pulse), the 14C was predominantly (99%) in the shoot biomass and declined thereafter. The results indicate that translocation of recent photoassimilate is rapid, with 14C detected below ground within 30 min of pulse application. The translocation rate of 14C below ground was maximal (6.2% h-1) between 30 min and 3 h after the pulse, with greatest incorporation into the microbial biomass detected at 3 h. After 3 h, the microbial biomass 14C pool accounted for 74% of the total 14C rhizosphere pool. By 24 h, approximately 30% of 14C assimilate had been translocated below ground; thereafter 14C translocation was greatly reduced. Partitioning of recent assimilate changed with increasing CO2 concentration. The proportion of 14C translocated below ground almost doubled from 17.76% at the ambient atmospheric CO2 concentration (450 ppm) to 33.73% at 750 ppm CO2 concentration. More specifically, these changes occurred in the root biomass and the total rhizosphere pools, with two- and threefold 14C increases at an elevated CO2 concentration compared to ambient, respectively. The pulselabelling strategy developed in this study provided sufficient sensitivity to determine perturbations in C dynamics in L. perenne, in particular rhizosphere C pools, in response to an elevated atmospheric CO2 concentration.  相似文献   

15.
We used a continuous labeling method of naturally 13C-depleted CO2 in a growth chamber to test for rhizosphere effects on soil organic matter (SOM) decomposition. Two C3 plant species, soybean (Glycine max) and sunflower (Helianthus annus), were grown in two previously differently managed soils, an organically farmed soil and a soil from an annual grassland. We maintained a constant atmospheric CO2 concentration at 400±5 ppm and δ13C signature at −24.4‰ by regulating the flow of naturally 13C-depleted CO2 and CO2-free air into the growth chamber, which allowed us to separate new plant-derived CO2-C from original soil-derived CO2-C in soil respiration. Rhizosphere priming effects on SOM decomposition, i.e., differences in soil-derived CO2-C between planted and non-planted treatments, were significantly different between the two soils, but not between the two plant species. Soil-derived CO2-C efflux in the organically farmed soil increased up to 61% compared to the no-plant control, while the annual grassland soil showed a negligible increase (up to 5% increase), despite an overall larger efflux of soil-derived CO2-C and total soil C content. Differences in rhizosphere priming effects on SOM decomposition between the two soils could be largely explained by differences in plant biomass, and in particular leaf biomass, explaining 49% and 74% of the variation in primed soil C among soils and plant species, respectively. Nitrogen uptake rates by soybean and sunflower was relatively high compared to soil C respiration and associated N mineralization, while inorganic N pools were significantly depleted in the organic farm soil by the end of the experiment. Despite relatively large increases in SOM decomposition caused by rhizosphere effects in the organic farm soil, the fast-growing soybean and sunflower plants gained little extra N from the increase in SOM decomposition caused by rhizosphere effects. We conclude that rhizosphere priming effects of annual plants on SOM decomposition are largely driven by plant biomass, especially in soils of high fertility that can sustain high plant productivity.  相似文献   

16.
Below-ground transfer of nitrogen (N) fixed by legume trees to associated non-N2-fixing crops has received little attention in agroforestry, although the importance of below-ground interactions is shown in other ecosystems. We used 15N natural abundance to estimate N transfer from the legume tree Gliricidia sepium (Jacq.) Kunth ex Walp. to C4 grass Dichanthium aristatum (Poir.) C.E. Hubb. in a silvopastoral system, where N was recycled exclusively by below-ground processes and N2 fixation by G. sepium was the sole N input to the system. Finding a suitable reference plant, a grass without contact with tree roots or litter, was problematic because tree roots invaded adjacent grass monocrop plots and soil isotopic signature in soil below distant grass monocrops differed significantly from the agroforestry plots. Thus, we used grass cultivated under greenhouse conditions in pots filled with agroforestry soil as the reference. A model of soil 15N fractionation during N mineralization was developed for testing the reliability of that estimate. Experimental and theoretical results indicated that 9 months after greenhouse transplanting, the percentage of fixed N in the grass decreased from 35% to <1%, due to N export in cut grass and dilution of fixed N with N taken up from the soil. The effect of soil 15N fractionation on the estimate of the reference value was negligible. This indicates that potted grass is a suitable reference N transfer studies using 15N natural abundance. About one third of N in field-grown grass was of atmospheric origin in agroforestry plots and in adjacent D. aristatum grassland invaded by G. sepium roots. The concentration of fixed N was correlated with fine root density of G. sepium but not with soil isotopic signature. This suggests a direct N transfer from trees to grass, e.g. via root exudates or common mycorrhizal networks.  相似文献   

17.
Sources of CO2 efflux from soil and review of partitioning methods   总被引：7，自引：0，他引：7  
Five main biogenic sources of CO2 efflux from soils have been distinguished and described according to their turnover rates and the mean residence time of carbon. They are root respiration, rhizomicrobial respiration, decomposition of plant residues, the priming effect induced by root exudation or by addition of plant residues, and basal respiration by microbial decomposition of soil organic matter (SOM). These sources can be grouped in several combinations to summarize CO2 efflux from the soil including: root-derived CO2, plant-derived CO2, SOM-derived CO2, rhizosphere respiration, heterotrophic microbial respiration (respiration by heterotrophs), and respiration by autotrophs. These distinctions are important because without separation of SOM-derived CO2 from plant-derived CO2, measurements of total soil respiration have very limited value for evaluation of the soil as a source or sink of atmospheric CO2 and for interpreting the sources of CO2 and the fate of carbon within soils and ecosystems. Additionally, the processes linked to the five sources of CO2 efflux from soil have various responses to environmental variables and consequently to global warming. This review describes the basic principles and assumptions of the following methods which allow SOM-derived and root-derived CO2 efflux to be separated under laboratory and field conditions: root exclusion techniques, shading and clipping, tree girdling, regression, component integration, excised roots and insitu root respiration; continuous and pulse labeling, 13C natural abundance and FACE, and radiocarbon dating and bomb-14C. A short sections cover the separation of the respiration of autotrophs and that of heterotrophs, i.e. the separation of actual root respiration from microbial respiration, as well as methods allowing the amount of CO2 evolved by decomposition of plant residues and by priming effects to be estimated. All these methods have been evaluated according to their inherent disturbance of the ecosystem and C fluxes, and their versatility under various conditions. The shortfalls of existing approaches and the need for further development and standardization of methods are highlighted.  相似文献   

18.
Despite its potential impact on soil carbon flow, few studies have attempted to quantify the effects of elevated carbon dioxide (CO2) on production of exudates by mycorrhizal plants. In this study we quantified low molecular weight (LMW) organic compounds exuded by non-mycorrhizal (NM) and ectomycorrhizal (ECM) plants in relation to exposure to elevated CO2. Scots pine seedlings, either colonized by one of eight different ECM fungi or non-mycorrhizal (NM), were exposed to either ambient (350 ppm) or elevated (700 ppm) concentrations of CO2. Exudation of LMW organic acids (LMWOAs), amino acids, dissolved monosaccharides and total dissolved organic carbon (DOC) was determined and exudation rates were calculated per g root and fungal dry mass. CO2 had a significant impact on exudation. Under elevated CO2, exudation of total LMWOAs increased by 120-160%, amino acids by 250%, dissolved monosaccharides by 130-270% and DOC by 180-220% compared to ambient CO2 treatment. Net CO2 assimilation rates increased significantly by 41-47% for seedlings exposed to elevated CO2. Exuded C calculated as a percentage of assimilated CO2 increased by 41-88% in the elevated CO2 treatment compared to ambient CO2 treatment.  相似文献   

19.
【目的】采用15N、13C同位素示踪技术,通过对不同施氮量下嘎啦幼苗生长状况及氮、碳分配、利用特性等的研究,以期为苹果生产合理施肥提供依据。【方法】将2年生盆栽嘎啦幼苗进行低、中、高三个氮水平处理,同时进行15N标记。在新梢旺长初始期、新梢旺长期、新梢缓长期分别进行整株13C标记,72小时后,整株解析为叶、梢、根三部分,进行15N、13C测定。样品全氮用凯氏定氮法测定,15N丰度用ZHT-03质谱计测定。13C丰度用DELTA V Advantage同位素比率质谱仪测定。【结果】1)中、高氮水平的施肥处理可在不同程度上提高整株及叶片干物质量和新梢长度。新梢旺长初始期和新梢缓长期嘎啦幼苗整株干物质量、新梢旺长期叶片干物质分配比率在中、高氮水平处理间差异不显著,中氮水平经济有效。新梢旺长期以后新梢长度以中氮高氮低氮,三者间差异性显著,中氮处理有利于新梢生长。2)在新梢旺长初始期,低氮处理植株叶片15N分配率达50%,比其他处理高出13个百分点左右,表明低氮处理更多的氮被叶片所利用,中氮和高氮处理间差异不显著,说明在本试验施氮条件下中氮供应水平已能满足氮素营养需求。3)新梢旺长期和新梢缓长期幼苗13C固定量均以中氮处理最高,新梢旺长初始期3个处理间根系13C分配率中氮高氮低氮,表明中氮处理有利于碳同化物在嘎啦幼苗中的分配。4)不同施氮量处理的嘎啦幼苗,15N利用率随施氮水平提高而降低,高氮处理对碳同化物分配没有显著贡献。【结论】低、中、高氮不同处理新梢缓长期碳同化物在各器官间的分配比较均衡,氮素水平不能影响碳同化物的分配。盆栽试验表明,中氮水平在保证营养供应的同时,能够促进新梢生长和树势健壮。  相似文献   

20.
Summary A lysimetric experiment was performed in a greenhouse to evalute root deposition and net release of soluble organic compounds after 1 and 2 years from pine and beech seedlings inoculated with an ectomycorrhizal fungus (Laccaria laccata) and/or rhizobacteria (Agrobacterium radiobacter for beech and Agrobacterium sp. for pine). Total C compounds released in the rhizosphere of both plants increased after inoculation with the bacteria or ectomycorrhizal fungus. The rhizobacteria increased root and plant growth and rhizodeposition, but the mycorrhizal fungi appeared to increase only root deposition. Soluble C compounds, collected after 2 years, represented only 0.1–0.3% of the total C compounds released into the rhizosphere, and were modified by inoculation with the microorganisms. After inoculation with the bacteria, levels of sugars and amino acids decreased in pine and beech rhizospheres, whereas organic acids increased, especially in the pine rhizosphere. In the rhizosphere of mycorrhizal beeches, sugar and amino acids increased, and organic acids differed from those released from non-mycorrhizal beeches. In the mycorrhizal pine rhizosphere, however, all compounds decreased. Following dual inoculations, mycorrhizal colonization increased, no effect on plant growth was observed, and virtually no organic acids were detected.  相似文献   

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