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1.
Genetic analysis of leaf-rust resistance was conducted on two durum wheats. Triticum durum cvs. ‘PBW 34’ and ‘DWL 5023’ were crossed with the leaf-rust-susceptible durum wheat ‘Malvi Local’. The F1, F2 and F3 generations were tested against leaf-rust pathotypes 1, 77A and 108. In ‘PBW 34’, a single dominant gene was effective against each of the pathotypes 1 and 108, whereas two independently inherited dominant genes were effective against pathotype 77A. In ‘DWL 5023’, two independently inherited dominant genes were operative against pathotypes 1 and 77A, whereas a single dominant gene was identified as being operative against pathotype 108. Allelic tests on F2 generation and joint segregation analysis on F3 generation seedlings, suggested that two different genes in each cultivar are effective against these three leaf-rust pathotypes. Cultivar ‘PBW 34’ has Lrd1 and Lrd2 genes whereas Lrd1 and Lrd3 genes are present in ‘DWL 5023’. 相似文献
2.
Inheritance of resistance to Verticillium wilt (Verticillium dahliae) in cotton (Gossypium hirsutum L.) 总被引：2，自引：0，他引：2
Verticillium wilt, caused by Verticillium dahliae Kleb., is a major constraint to cotton production in almost all countries where cotton is cultivated. Developing new cotton cultivars resistant to Verticillium wilt is the most effective and feasible way to combat the problem. Little is known about the inheritance of resistance to Verticillium wilt of cotton, especially that caused by the defoliating (D) and nondefoliating (ND) pathotypes of the soil‐borne fungus V. dahliae. The objective of this study was to determine the inheritance of resistance in cotton against both pathotypes of V. dahliae. Crosses were made between the susceptible parent ‘Cukurova 1518’ and each of four resistant parents PAUM 401, PAUM 403, PAUM 405 and PAUM 406 to produce F2 generations in 2002 and F2:3 families in 2003. Disease responses of parent and progeny populations to the D and ND pathotypes were scored based on a scale of 0‐4 (0, resistant; 4, susceptible). F2 populations inoculated with the D pathotype showed a 3 : 1 (resistant : susceptible) plant segregation ratio. Tests of F2:3 families confirmed that resistance was controlled by a single dominant gene. In contrast, analysis of data from F2‐ and F2‐derived F3 families suggested that resistance to the ND pathotype is controlled by dominant alleles at two loci. 相似文献
3.
A. N. Mishra K. Kaushal G. S. Shirsekar S. R. Yadav R. N. Brahma H. N. Pandey 《Plant Breeding》2005,124(5):514-516
The bread wheat cultivar ‘Thatcher’ is documented to carry the gene Lr22b for adult‐plant resistance to leaf rust. Seedling‐resistance to leaf rust caused by Puccinia triticina in the bread wheat cultivar ‘Thatcher’, the background parent of the near‐isogenic lines for leaf rust resistance genes in wheat, is rare and no published information could be found on its genetic basis. The F2 and F3 analysis of the cross ‘Agra Local’ (susceptible) × ‘Thatcher’ showed that an apparently incompletely dominant gene conditioned seedling‐resistance in ‘Thatcher’ to the three ‘Thatcher’‐avirulent Indian leaf rust pathotypes – 0R8, 0R8‐1 and 0R9. Test of allelism revealed that this gene (temporarily designated LrKr1) was derived from ‘Kanred’, one of the parents of ‘Thatcher’. Absence of any susceptible F2 segregants in a ‘Thatcher’ × ‘Marquis’ cross confirmed that an additional gene (temporarily designated LrMq1) derived from ‘Marquis’, another parent of ‘Thatcher’, was effective against pathotype 0R9 alone. These two genes as well as a second gene in ‘Kanred’ (temporarily designated LrKr2), which was effective against all the three pathotypes, but has not been inherited by ‘Thatcher’, seem to be novel, undocumented leaf rust resistance genes. 相似文献
4.
The inheritance of the Mlk powdery mildew resistance originating from ‘Heine 2174.50’ was analyzed by crossing the Mlk resistant cultivar ‘Ralle’× cv. ‘Amor’ (highly susceptible) and vice versa and by observing the reactions of F1- and F2-plants after inoculation with two different Mlk avirulent powdery mildew isolates. In all cases, a 3 (resistant): I (susceptible) segregation was found in F2. The reactions of the F2plants against the two powdery mildew isolates were identical in each case. Therefore, it is supposed that one dominant resistant gene is responsible for the resistant reactions against these two isolates. These results support the earlier assumption of Heun and Fischbeck (1987b) that the whole Mlk resistance pattern is controlled by a single gene. 相似文献
5.
Summary The genetic constitution of two bread wheat accessions from the International Spring Wheat Rust Nurseries (E 5883 and E 6032) has been studied for reaction to four Indian races of stem rust. Analysis of E 5883 has revealed that for each of the races 15C, 21 and 40 a single dominant gene operates for resistance. The dominant gene against race 15C was identified as Sr6. The dominant genes for resistance against races 21 and 40 were found to be different from the genes described so far. Resistance against race 122 is controlled by a single recessive gene producing characteristically a 2 type of reaction. This gene was identified as Sr8.The resistance of E 6032 against each of the races 15C, 21 and 40 is controlled by two genes, one dominant and one recessive, which act independently. Dominant genes effective against 15C, 21 and 40 were conclusively identified as Sr6, Sr5 and Sr9b, respectively. From the correlated behaviour against races 15C and 40 as well as from the phenotypes of the resistance reactions rhe same recessive gene, undescribed so far, operates against the two races. The second recessive gene operating against race 21 was also observed to be different from those so far designated. E 6032 was, however, found to be susceptible to races 122.The presence of Sr6 both in E 5883 and E 6032 against race 15C was further confirmed through F2 and F3 segregation data. 相似文献
6.
Summary Resistance to zucchini yellow mosaic virus has been found in the muskmelon line PI 414723 from India. This resistance is effective against the ZYMV strains E15 and 1318 belonging respectively to the NF and F pathotypes. Resistance to E15 (no vein clearing and yellowing symptoms) is governed by one dominant gene (symbol Zym) according to segregations observed in F1, F2 and BC1 progenies. This gene is epistatic dominant over Fn, which induces wilting and necrosis after inoculation with F pathotype. Linkage studies suggest that Zym inherits independently from Fom-1, Fom-2, Vat, Wmv and Fn but is linked with a (13.1 ±2.4 units). 相似文献
7.
Resistance to the prevailing races of sunflower rust, Puccinia hehanthi Schw., is lacking in the commercial hybrids (Helianthus annuus L.). The objective of this study was to identify new sources of resistance to the four North American rust races in wild Helianthus species, and to determine their mode of inheritance. Seventy-eight accessions of H. annuus L., H. argophyllus Torrey and Gray, and H. petiolans Nutt. were evaluated in the greenhouse. Resistance to races 1, 2, 3, and 4 was observed in 25, 28, 15, and 26% of the plants, respectively, and 10% of the plants were resistant to all four races. Seven accessions that had a high percentage of resistant plants to all the four races were selected and one resistant plant from each accession was crossed with susceptible inbred line HA89. Three to four F1 plants resistant to all four races from each cross were backcrossed with HA89. F1 plants from PI-413118 × HA89 and PI 413175 × HA89 were resistant to all four races. The PI 413023 × HA89 F1 plants were 100 % resistant to races 3 and 4 and segregated in a 3: 1 resistant (R) to susceptible (S) ratio to races 1 and 2. The other four F1 combinations segregated 3R: IS ratios to all four races. Bc1F1 progenies revealed that plants from PI 413048, PI 413037, PI 413038, and PI 413171 used in the crosses possessed two dominant genes in heterozygous condition for resistance to each of the four races, whereas plants from PI 413023 possessed two dominant genes in heterozygous condition for resistance to each of races 1 and 2, and one dominant resistance gene in homozygous condition for each of races 3 and 4. Plants from PI 413118 and PI 413175 carried a single dominant gene in homozygous condition for resistance against each of the four races. 相似文献
8.
小麦新种质CH09W83为八倍体小偃麦TAI7047与高感小麦品种晋太170杂交、回交后代衍生而来的高代选系,在苗期免疫或高抗我国白粉病菌株E09、E20、E21、E23、E26、Bg1和Bg2。为定位CH09W83中的抗病基因,将CH09W83与感病亲本杂交和回交,通过对F1、F2、F2:3和BC1代的接种鉴定和遗传分析,证实CH09W83成株期对E09的抗性由1对隐性核基因控制,暂命名为pmCH83。采用分离群体分组分析法(bulked segregant analysis, BSA),以658对SSR标记对台长29(感病)× CH09W83的F2群体分析发现,抗性基因pmCH83与SSR标记Xgpw7272、Xwmc652、Xgwm251、Xgwm193连锁,与两翼邻近标记Xwmc652和Xgwm251的遗传距离分别为3.8 cM和4.3 cM。利用中国春缺体–四体、双端体将pmCH83及其连锁标记定位在4BL染色体上。原位杂交、染色体配对及连锁标记分析结果表明,CH09W83可能是一个小麦与中间偃麦草的隐形异源渗入系。系谱和图谱位置分析表明,pmCH83很可能是来自中间偃麦草一个新的抗白粉病基因。 相似文献
9.
Annet Namayanja Robin Buruchara George Mahuku Patrick Rubaihayo Paul Kimani Stephen Mayanja Hellen Eyedu 《Euphytica》2006,151(3):361-369
Inheritance of resistance to angular leaf spot (ALS) disease caused by Phaeoisariopsis griseola (Sacc.) Ferr was investigated in two common bean cultivars, Mexico 54 and BAT 332. Both Andean and Mesoamerican backgrounds were used to determine the stability of the resistance gene in each of the two cultivars. Resistance to P. griseola was phenotypically evaluated by artificial inoculation with one of the most widely distributed pathotypes, 63–39. Evaluation of the parental genotypes, F1, F2 and backcross populations revealed that the resistance to angular leaf spot in the cultivars Mexico 54 and BAT 332 to pathotype 63–39 is controlled by a single dominant gene, when both the Andean and Mesoamerican backgrounds were used. Allelism test showed that ALS resistance in Mexico 54 and BAT 332 to pathotype 63–39 was conditioned by the same resistance locus. Resistant and susceptible segregating populations generated using Mexico 54 resistant parent were selected for DNA extraction and amplification to check for the presence /absence of the SCAR OPN02 and RAPD OPE04 markers linked to the Phg-2 resistance gene. The results indicated that the SCAR OPN02 was not polymorphic in the study populations and therefore of limited application in selecting resistant genotypes in such populations. On the other hand, the RAPD OPE04 marker was observed in all resistant individuals and was absent in those scored susceptible based on virulence data. Use of the RAPD OPE04 marker in marker-assisted selection is underway. 相似文献
10.
Summary Inheritance studies on the stem termination in pigeon pea using F1, F2 and F3 generations of two crosses between determinate and indeterminate lines suggested that two dominant genes with epistatic (inhibitory) interaction of one of them control the interminate growth habit. The gene symbols D. idid and ddIdId have been designated to the parental plants with determinate and indeterminate growth habits, respectively. The gene IdId was epistatic (inhibitory) to the gene D giving a ratio of 13 indeterminate: 3 determinate inthe F2's observed. F3 segregation supported the proposed model on the mode of inheritance. 相似文献
11.
Sources of resistance to Indian pathotypes of Puccinia graminis tritici and P. triticina in durum wheat 
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下载免费PDF全文 Akhilesh N. Mishra Gautam S. Shirsekar Sitaram Yadav Kamini Kaushal Vijay G. Dubey Sakuru V. Sai Prasad 《Plant Breeding》2015,134(5):508-513
Stem rust and leaf rust are important diseases affecting durum wheat production in India. Inheritance and extent of diversity in resistance were studied in five durum genotypes, viz. ?B 662', ?ED 2398‐A', ?HG 110', ?IWP 5019' and ?Line 1172? using Pgt pathotypes 40A and 117‐6, and Pt pathotypes 12‐2 and 104‐2. F2 and F3 analyses showed that resistance was conferred by one or two genes in each line. In all, four genes for resistance to 40A, and eight each for pathotypes 117‐6 and 12‐2 were identified among the five genotypes, and three for resistance to 104‐2 among B 662, ED 2398‐A and IWP 5019 were indicated by tests of allelism. Although the gene identities are not known, at least some of them should be different from Sr2, Sr7b, Sr8a, Sr8b, Sr9e, Sr9g, Sr11, Sr12, Sr13, Sr14, Sr17, Sr23 and Sr28; and from Lr3, Lr14a, Lr23 and Lr27 + Lr31. These genotypes enrich the diversity of resistance to stem rust and leaf rust for durum wheat improvement. 相似文献
12.
Inheritance and allelic relationship among gene(s) for blast resistance in pearl millet [Pennisetum glaucum (L.) R. Br.] 下载免费PDF全文
下载免费PDF全文 Shweta Singh Rajan Sharma Bheemavarapu Pushpavathi Shashi Kumar Gupta Ch. V. Durgarani Chandramani Raj 《Plant Breeding》2018,137(4):573-584
Six blast‐resistant pearl millet genotypes, ICMB 93333, ICMB 97222, ICMR 06444, ICMR 06222, ICMR 11003 and IP 21187‐P1, were crossed with two susceptible genotypes, ICMB 95444 and ICMB 89111 to generate F1s, F2s and backcrosses, BC1P1 (susceptible parent × F1) and BC1P2 (resistant parent × F1) for inheritance study. The resistant genotypes were crossed among themselves in half diallel to generate F1s and F2s for test of allelism. The F1, F2 and backcross generations, and their parents were screened in a glasshouse against Magnaporthe grisea isolates Pg 45 and Pg 53. The reaction of the F1s, segregation pattern of F2s and BC1P1 derived from crosses involving two susceptible parents and six resistant parents revealed the presence of single dominant gene governing resistance in the resistant genotypes. No segregation for blast reaction was observed in the F2s derived from the crosses of resistant × resistant parents. The resistance reaction of these F2s indicated that single dominant gene conferring resistance in the six genotypes is allelic, that is same gene imparts blast resistance in these genotypes to M. grisea isolates. 相似文献
13.
Zhi-Wei Wang Lei Gao Hai Zhou Liu Shi Yong Mei Yuan Zhou Chang Ping Xiang Ting Wang 《Euphytica》2012,186(2):313-320
A male sterile plant appeared in the radish breeding program at the Hubei Academy of Agricultural Sciences, Hubei, China.
In its progeny, a two-type (half of plants male sterile, the other half male fertile) line 01GAB was established. An F2 population of 260 plants from a cross of male-sterile 01GAB and a male fertile line 9802H segregated for male fertility in
a 3:1 ratio indicating that fertility was restored by a single dominant gene, here designated RsMs. A PCR-based DNA marker specific to the male fertility Rfob gene in 9802H was absent in 01GAB. Linkage analysis placed the RsMs locus 10.7 cM away from the Rfo locus. In an F2 population of hybrids between 01GAB and male fertile 9802B, a co-dominant DNA marker for the RSultr3.2A (a radish sulfate transporter gene) locus was linked to the RsMs locus at 1.5 cM suggesting that fertility restoration in 01GAB was located in the region with known male sterility restorers
in radish. However, no maintainer for the 01GAB source of male sterility has been identified so far. Cytological observations
have shown that the abnormalities in male sterile anthers first appeared in tapetum at the tetrad stage, followed by a hypertrophy
of the tapetal cells at the vacuolate microspore period. These results suggest that male sterility in 01GAB is likely to be
genetic in nature, or it may represent a new type of the cytoplasmic male sterility. 相似文献
14.
The purpose of this study was to elucidate the genetic cause for the lack of express ion of the gene Ht2 against Exserohilum turcicum race 2 on certain genetic backgrounds related to the inbred line B14. Two such inbreds (A635Rp and B59), susceptible to this pathogen were crossed onto the inbred Oh43—-Ht2Ht2. The following generations: F1, F2, F3, F4, backcrosses to both parents, selfings of the backcrosses, in addition to the parent lines, were evaluated for Ht2 expression in this study. Plants of each generation were inoculated with E. turcicum race 2 and evaluated for the expression of the chlorotic-lesion resistance determined by the gene Ht2. In spite of the reported dominance of Ht2 on some genetic backgrounds, the F1 generations studied here did not show Ht2 resistant lesions. The data presented herein suggest that B14 and related inbred lines carry a dominant gene(I) that inhibits the expression of the Ht2 gene. Chi-square analyses of the reactions of 108 progeny families studied supported this hypothesis. 相似文献
15.
Yan-an Guan Hai-lian Wang Ling Qin Hua-wen Zhang Yan-bing Yang Feng-ju Gao Ru-yu Li Hong-gang Wang 《Euphytica》2011,182(3):431-440
Plant height (PHT), stem and leaf fresh weight (SLFW), juice weight (JW) and sugar content of stem (Brix) are important traits
for biofuel production in sweet Sorghum. QTL analysis of PHT, SLFW, JW and Brix was conducted with composite interval mapping using F2 and F2:3 populations derived from the cross between grain Sorghum (Shihong137) × sweet Sorghum (L-Tian). Three QTLs controlling PHT were mapped on SBI-01, SBI-07 and SBI-09 under four different environments. These QTLs
could explain 10.16 to 45.29% of the phenotypic variance. Two major effect QTLs on SBI-07 and SBI-09 were consistently detected
under four environments. Eight QTLs controlling SLFW were mapped across three environments and accounted for 5.49–25.36% of
the phenotypic variance. One major QTL on SBI-09 located between marker Sb5-206 and SbAGE03 was observed under three environments.
Four QTLs controlling Brix were identified under two environments and accounted for 11.03–17.65% of the phenotypic variance.
Six QTLs controlling JW were detected under two environments, and explained 6.63–23.56% of the phenotypic variance. QTLs for
JW on SBI-07 and SBI-09 were consistent in two environments showing higher environmental stability. In addition, two chromosome
regions on SBI-07 and SBI-09 were identified in our study having major effect on PHT, SFLW and JW. The results would be useful
for the genetic improvement of sweet Sorghum to be used for biofuel production. 相似文献
16.
Summary In studies of the inheritance of resistance, pea seedlings of seven lines in which stems and leaves were both resistant to Mycosphaerella pinodes were crossed with a line in which they were both susceptible. With seven of the crosses resistance was dominant to susceptibility. When F2 progenies of five crosses were inoculated on either stems or leaves independently, phenotypes segregated in a ratio of 3 resistant: 1 susceptible indicating that a single dominant gene controlled resistance. F2 progenies of one other cross gave ratios with a better fit to 9 resistant: 7 susceptible indicating that two co-dominant genes controlled resistance. The F2 progeny of another cross segregated in complex ratios indicating multigene resistance.When resistant lines JI 97 and JI 1089 were crossed with a susceptible line and leaves and stems of each F2 plant were inoculated, resistance phenotypes segregated independently demonstrating that leaf and stem resistance were controlled by different genes. In two experiments where the F2 progeny of the cross JI 97×JI 1089 were tested for stem and leaf resistance separately, both characters segregated in a ratio of 15 resistant:1 susceptible indicating that these two resistant lines contain two non-allelic genes for stem resistance (designated Rmp1 and Rmp2) and two for leaf resistance (designated Rmp3 and Rmp4). Evidence that the gene for leaf resistance in JI 1089 is located in linkage group 4 of Pisum sativum is presented. 相似文献
17.
Summary F2 plants of five, and F3 plants of three, crosses between genotypes carrying the race-specific resistance gene Xa-4 and genotypes not carrying this gene were inoculated with two isolates of Xanthomonas campestris pv. oryzae. Half the tillers of each plant received isolate PX061, avirulent on the Xa-4 gene, the other half of the tillers received isolate PX099, virulent for the Xa-4 gene. The F2 and F3 populations segregated for a single dominant resistance gene, Xa-4.The parental, F2 and F3 genotypes not carrying Xa-4 had mean lesion lengths between 28 and 29 cm for both isolates. The Xa-4 carrying parents showed a mean lesion length of 2.7 cm with the avirulent isolate and of 12.4 cm with the virulent isolate. The Xa-4 carrying F2 and F3 genotypes had mean lesion lengths of 5.2 and 20.1 cm for the two isolates, respectively. These observations strongly indicate that the Xa-4 gene, carried by the rice genotypes studied (IR28, Cisadane and BR51-282-8), had a considerable residual effect when exposed to virulent isolate PXO99. 相似文献
18.
小麦新种质CH7124由八倍体小偃麦TAI8335与高感白粉病小麦品种晋麦33杂交后代衍生而来,在苗期对白粉病菌株E09、E20、E21、E23、E26、Bg1和Bg2表现免疫或高抗,抗病表现与TAI8335及其野生亲本中间偃麦草相似。基因组原位杂交未检测到CH7124含有外源染色体信号。利用CH7124与感病亲本SY95-71和绵阳11的杂交群体接种鉴定和遗传分析证实,CH7124成株期对E09的抗性由1对显性核基因控制,暂命名为Pm CH7124。采用分离群体分组分析法(bulked segregant analysis,BSA)对SY95-71/CH7124的F6群体进行SSR标记扫描,发现抗性基因Pm CH7124与5对SSR标记连锁,与两翼邻近标记Xgwm501和Xbarc101的遗传距离分别为1.7 c M和4.5 c M。利用中国春缺体–四体和双端体材料,将Pm CH7124及其连锁标记定位在小麦2B染色体长臂上。通过分析2BL上其他抗白粉病基因的抗谱、抗性来源、物理图谱位置以及连锁标记在Pm CH7124作图群体中的多态性,认为Pm CH7124不同于2BL上已知的抗白粉病基因Pm6、Pm33、Pm JM22、Ml Zec1、Ml AB10和Ml LX99。 相似文献
19.
S. C. Gupta 《Plant Breeding》1994,112(2):135-140
Screening of a population of Ocimum basilicum var. glabratum Benth., raised from seeds collected from France, West Africa and Kerala (India) was carried out for chemical composition of its essential oils. In all three collections, plants were found to be comprised of 3 chemotypes which were morphologically indistinguishable, but each chemotype was found to be capable of intercrossing freely with the other two. Selfing was carried out during 4 consecutive generations, after which three pure lines were established. Study of the selfed progeny of each collection exhibited an interesting segregation in S2 and S3 generations. Along with segregation in F2 and F3 generations of various hybrids the results can be explained by assuming a gene ‘M’ existing in 3 (or more) allelic forms: The allele M0 for biosynthesis of estragole (methyl chavicol) is dominant to allelcs M1 , for eugenol and M2 for camphor. Also, allele M1 is dominant to allele M2 . The assumption was further confirmed by testcrosses of F1 hybrids of each cross with their recessive parent. 相似文献
20.
The fungus Pyrenophora tritici-repentis, causal agent of tan spot of wheat, produces two phenotypically distinct symptoms, tan necrosis and extensive chlorosis. The inheritance of resistance to chlorosis induced by P. tritici-repentis races 1 and 3 was studied in crosses between common wheat resistant genotypes Erik, Hadden, Red Chief, Glenlea, and 86ISMN 2137 and susceptible genotype 6B-365. Plants were inoculated under controlled environmental conditions at the two-leaf stage and disease rating was based on presence or absence of chlorosis. In all the resistant × susceptible crosses, F1 plants were resistant and the segregation of the F2 generation and F3 families indicated that a single dominant gene controlled resistance. Lack of segregation in a partial diallel series of crosses among the resistant genotypes tested with race 3␣indicated that the resistant genotypes possessed␣the same resistance gene. This resistance gene was effective against chlorosis induced by P.␣tritici-repentis races 1 and 3. 相似文献