Effect of herbage water-soluble carbohydrate content and weather conditions at ensilage on the fermentation of grass silages made on commercial farms     

P. M. HAIGH 《Grass and Forage Science》1990,45(3):263-271

A total of 1009 samples of silage made in bunker silos on commercial farms between 1972 and 1978 was analysed to investigate the effect of herbage water-soluble carbohydrate content (WSC) and weather conditions at ensilage on fermentation as measured by ammonia-N concentration and pH of first-cut grass silages.
Silage dry matter (DM) content had the major effect on fermentation. Factors influencing silage DM were rainfall and hours of sunshine during silage making, and DM content of the grass cut. WSC content of herbage ensiled also had a significant effect on subsequent fermentation. The major influences on herbage WSC were hours of sunshine and rainfall during the growing season.
The effect of chemical additives, albeit at poorly defined and often inadequate rates, was small in comparison to that of silage DM.
The minimum DM necessary to produce well-fermented silage without additive was approximately 260 g kg⁻¹. Use of formic acid significantly reduced this requirement to 240 g kg⁻¹ and to 252 g kg⁻¹ for sulphuric add + formalin. The results indicate that the minimum herbage WSC necessary to prevent a clostridial fermentation developing in silage with a DM content of 230 g kg⁻¹ is approximately 37 g kg⁻¹ without additive and 30 g kg⁻¹ with formic acid.
It is concluded that on commercial farms, weather conditions i.e. amount of rainfall and sunshine prior to and at ensilage, have a greater effect on subsequent silage fermentation than additive use.  相似文献   

An evaluation of three bacterial inoculants and formic acid as additives for first harvest grass   总被引：1，自引：0，他引：1  

S. J. KENNEDY 《Grass and Forage Science》1990,45(3):281-288

An experiment was carried out during 1984 to study the effect of treating grass at ensiling with three commercially available inoculant-type additives (H/M Inoculant, Grass Sile and Siron), formic acid (850 g kg⁻¹; Add-F) or no additive on grass preservation, in-silo loss, intake and animal performance. Primary growth grass ensiled from 28–29 May into concrete-walled covered silos was of high dry matter (DM, 234 g kg⁻¹), water-soluble carbohydrate content (WSC. 212 g kg DM⁻¹) and digestibility (MADF, 250 g kg DM⁻¹).
The untreated silage displayed good preservation and with the exception of the Sirontreated silage which showed significantly lower buffering capacity (Be) and volatile fatty acid (VFA) contents than the untreated silage, the application of inoculant-type additives did not improve silage preservation or decrease in-silo DM losses. The formic acid-treated silage displayed significantly lower Be, water-soluble carbohydrate, ash, ammonia nitrogen (g kg total N⁻¹) and lactate contents than the untreated silage.
After a 133 d storage period, silages were offered to finishing beef cattle for an 84-d period. Cattle offered the silages displayed similar and non-significant daily DM intakes, daily liveweight gains, dressing proportions and daily carcass gains. From this experiment it appears unlikely that any of the additives evaluated will improve animal performance relative to a well-preserved untreated silage.  相似文献   

Effects of treating lucerne with an inoculum of lactic acid bacteria or formic acid upon chemical changes during fermentation, and upon the nutritive value of the silage for lambs   总被引：4，自引：0，他引：4  

L. E. PHILLIP  L. UNDERHILL  H. GARINO 《Grass and Forage Science》1990,45(3):337-344

A second cut of lucerne was wilted to 500 g DM kg⁻¹ and either left untreated (control) or treated with formic acid (4.5 1 fresh forage t⁻¹) or with a commercial inoculum of lactic acid bacteria (10⁵ colony forming units (cfu) g forage⁻¹). The forages were ensiled in 2-t capacity silos for 8 months, and later fed to six lambs (mean initial weight 27.7 ±1.60 kg) in a 3x3 duplicated Latin square with 27-d periods. Portions of the untreated and additive-treated forages were also ensiled in laboratory silos at 25 ° C for intervals up to 42 d. Results from the laboratory silos showed that the major increase in ammonia-N in silage occurred between 40 h and 7 d of fermentation; during this period, both formic acid and the inoculant produced a smaller increase in ammonia-N, than did the control. The pH of inoculated silage declined from 5.74 to 4.57 in 7 d, but it took 14 d for the pH of the control silage to fall below 5.0. Formic acid treatment immediately reduced the silage pH from 5.74 to 5.10 ( P < 0·01); the pH then remained unchanged until 21 d, after which it decreased slightly. When compared with control, lambs fed formic acid-treated silage consumed more ( P < 0·05) digestible organic matter; the response was associated with a trend towards decreased concentration of ammonia in plasma. Inoculation of lucerne silage did not ( P < 0·05) affect voluntary intake but increased ( P <0.05) apparent digestibility of fibre and tended to increase N retention.  相似文献   

The effect of addition of sugar and inoculation with two commercial inoculants on the fermentation of lucerne silage in laboratory silos   总被引：10，自引：0，他引：10  

D. R. SEALE  A. R HENDERSON  K. O. PETTERSSON  J. F. LOWE 《Grass and Forage Science》1986,41(1):61-70

Lucerne (DM 236 g kg^-1, WSC 49 g (kg DM)^-1) was ensiled in test-tube silos with or without either glucose or fructose and with or without one of two commercial inoculants. The WSC content of the forage as ensiled was too low to obtain a well preserved untreated silage. By day 4 the pH values of the silages with added sugar or inoculant were significantly lower (P< 0·001) than the control silage. A satisfactory fermentation was attained only in the silages to which sugar and an inoculant had been added. These silages had a lower pH, more protein-N (P< 0·001), less ammonia-N (P<0·001), a faster increase in counts of lactic acid bacteria, and decrease in counts of coliforms than the other silages. Homo-fermentative lactic acid bacteria dominated the fermentation in the inoculated silages while leuconostocs dominated the early stages of fermentation in the control silages. The results indicate that if there is insufficient sugar in the original crop, then the bacteria in an inoculant will not be able to produce enough lactic acid to lower the pH to an acceptable level. This has important implications for the ensilage of lucerne and other highly buffered low sugar crops.  相似文献   

Effect of commercial inoculant and formic acid± formalin silage additives on silage fermentation and intake and on liveweight change of young cattle     

P. M. HAIGH  M. APPLETONT  S. F. CLENCH 《Grass and Forage Science》1987,42(4):405-410

Data from six experiments conducted at two Agricultural Development and Advisory Service Experimental Husbandry Farms during 1980–83 were used to compare low dry matter (DM, 160 g kg^-1), low water soluble carbohydrate (WSC, 15 g kg^-1), non-additive treated silage with silage treated with commercial inoculants or formic acid with or without added formalin (formic acid ± formalin). Formic acid ± formalin significantly decreased silage pH and ammonia-N and significantly increased silage residual WSC compared with inoculant or untreated silage. Formic acid ± formalin significantly increased oven DM and significantly reduced DM loss during ensiling compared with untreated silage. Formic acid ± formalin treatment significantly increased both silage DM and total DM intake compared with untreated silage. Daily liveweight gains of cattle offered formic acid ± formalin were significantly higher than those given inoculant or untreated silage.
It is suggested from the results that formic acid ± formalin additives can be used successfully to prevent a clostridial fermentation developing when crops contain 15 g WSC kg^-1.  相似文献   

A comparison of formic acid and an acid-salt type additive on the performance of dairy cows in early lactation     

O. D. DAVIES  P. M. HAIGH 《Grass and Forage Science》1993,48(1):64-69

Perennial ryegrass, harvested as second-cut material on 10 and 11 July 1990, was treated with either formic acid at 31 t^-1 or an acid-salt type additive at 61 t^-1 and ensiled in roofed 150 t bunker silos. Subsequently both silages underwent a predominantly lactic fermentation. Nevertheless the acid-salt-treated silage had a significantly higher quantity of formic acid (19 vs 12 g kg DM^-1) and significantly lower levels of lactic (98 vs 118 g kg DM^-1) and acetic acid (11 vs 17 g kg DM^-1) compared with formic acid-treated silage. In-silo losses and effluent production were similar.
Each silage was individually fed to 10 October-calving Friesian dairy cows (average weight 565 kg) from weeks 2 to 15 of lactation, together with 3 kg d^-1 of a compound feed containing 190 g kg DM^-1 crude protein and with an estimated metabolizable energy content of 12·6 MJ kg DM^-1. The acid-salt additive had no significant effect on silage DM intake, daily milk yield, milk protein or cow liveweight change, but significantly increased milk butterfat content compared with formic acid-treated silage.
It is concluded that the acid-salt type additive produced little difference in terms of either silage fermentation or animal performance compared with formic add treatment.  相似文献   

RESEARCH NOTE     

P. M. HAIGH†  D. G. CHAPPLE‡  T. L. POWELL† 《Grass and Forage Science》1996,51(3):318-323

Data from twenty-two experiments conducted at tour ADAS Research Centres during 1980–92 were used to compare untreated silages with silages treated with formic acid, with or without added formalin, commercial inoculants or molasses. The sillages were made from herbage whose dry-matter (DM) and water-soluble carbohydrate (WSC) contents were 277 (s.e. 0.46) g DM kg⁻¹ and 36 (s.e. 8.1) g kg⁻¹ respectively. Inoculant use significantly decreased silage pH and ammonia-N, significantly increased lactic acid and total acid content, and decreased butyric acid and total short-chain fatty acids. Formic acid use significantly increased silage lactic acid and total acid content, and decreased butyric acid content, whereas formic acid+formalin significantly decreased silage ammonia-N level. Molasses had little effect upon silage fermentation. Improvements in silage fermentation, however, produced little benefit in terms of either silage DM intake or liveweight gain when the silages were offered to growing lambs.
It is suggested from the results that inoculant- and formic acid-based additives can be used to improve the fermentation of big-bale silages.  相似文献   

The digestion by cattle of grass silages made with no additive or with the application of formic acid or formic acid and formaldehyde     

J. A. ROOKE  H. A. GREIFE  D. G. ARMSTRONG 《Grass and Forage Science》1983,38(4):301-310

The aim of the experiment was to assess the effect of applying, at commercially recommended rates, formic acid or formic acid/formaldehyde at ensilage upon the subsequent digestion of the silages by cattle. Three wilted grass silages were made from perennial ryegrass ensiled at a DM concentration of 206 g kg⁻¹ after a poor wilting period of 49 h without additive application and with application of 2.5 litres t⁻¹ of formic acid or of 4.5 litres t⁻¹ of a mixture of (gkg⁻¹) 500 formic acid, 200 sulphuric acid and 200 formatin giving an application rate of 15 g formaldehyde kg⁻¹ herbage crude protein (N × 6.25).
The silages were fed to cattle equipped with rumen cannulae and duodenal re-entrant cannulae. Results from analyses of silage composition and from the digestion of organic matter and N showed no major differences between silages. The efficiency of rumen microbial N synthesis, the rumen degradability of silage N (determined in vivo or in sacco ) and in vivo digestion of individual amino acids were also unaffected by additive treatment. These results indicate that poor wilting conditions before ensilage restricted the effectiveness of additive treatment; the ineffectiveness of formaldehyde in reducing the rumen degradability of silage N may also have been related to the low rate of formaldehyde application.  相似文献   

The effect of an acid salt-type additive on the fermentation of grass silages made in bunker silos on commercial dairy farms in Wales     

P. M. HAIGH 《Grass and Forage Science》1992,47(4):353-357

The fermentation characteristics and chemical composition of 57 first-cut and 30 second-cut samples of grass silages, made in bunker silos on commercial dairy farms in Wales in 1990, and treated with a nominal 61 t⁻¹ of an acid salt-type additive at ensilage, is described. Typical chemical composition of grass cut for ensilage was 156 g kg⁻¹ dry matter (DM) and 28 g kg⁻¹ water soluble carbohydrate (WSC), with 181 g (kg DM) ⁻¹ crude protein (CP) and 232 g (kg DM) ⁻¹ modified acid detergent fibre (MADF). The effect of additive use was to produce silages with DM 230 g kg⁻¹ pH 3·93, ammonia N 70 g kg⁻¹ total N, with residual WSC 35 g (kg DM) ⁻¹, lactic acid 83 g (kg DM) ⁻¹, total acids 118 g (kg DM) ⁻¹ and butyric acid 0·7 g (kg DM) ⁻¹. No significant differences were found between first- and second-cut silages. Silage fermentation was restricted (i.e. lactic acid less than 60 g kg DM⁻¹) in only 20% of the samples.
It is suggested that on commercial farms the application rate achieved may be insufficient to produce a restricted fermentation.  相似文献   

A study of the efficacy of a bacterial inoculant and formic acid as additives for grass silage in terms of milk production   总被引：2，自引：0，他引：2  

K. MARTINSSON 《Grass and Forage Science》1992,47(2):189-198

In a two-year experiment, three silages were prepared from herbage treated either with an inoculant at 1·25 × 10⁵ organisms (g fresh material (FM))⁻¹. formic acid (850 g kg⁻¹) at 4 1 (t FM)⁻¹, or no additive (untreated). In Experiment 1, unwilted and in Experiment 2, wilted silages were investigated and had mean dry matter (DM) and water soluble carbohydrate (WSC) concentrations at ensiling of 171 g kg⁻¹ and 17·6 g (kg FM)⁻¹ and 263 g kg⁻¹ and 25·1 g (kg FM)⁻¹, respectively. In Experiment 1, 45 and in Experiment 2, 54 individually fed cows were used to evaluate the silages in three-treatment, randomized-block design experiments. During weeks 4-12 of lactation the cows were offered silages ad libitum and during weeks 15-26 a constant amount of silage was fed. There were few major differences in chemical composition of the resulting silages. Formic acid had no effect on silage digestibility. Inoculant treatment increased digestibility when the grass had been wilted. The use of formic acid resulted in increased silage DM intake of 9% during weeks 4-12 of lactation in Experiment 1 but not in Experiment 2. The inoculant gave no increase in silage DM intake over the control in Experiment 1 but increased silage DM intake by 7% in Experiment 2. There was no significant response in milk yield to formic acid. In Experiment 2 the response in milk yield to inoculant treatment was significant both in weeks 4-12 of lactation (4%) and in weeks 15-26 of lactation (5%). It is concluded that the response in milk yield to the use of a specific inoculant appears to be mediated through increased intake of metabolizable energy (ME).  相似文献   

The effect of wilting and silage additives on the fermentation of autumn made grass silage ensiled in bunkers on commercial farms in South Wales 1983-85   总被引：1，自引：0，他引：1  

P. M. HAIGH 《Grass and Forage Science》1988,43(3):337-345

Silage making practices in respect of 130 samples of autumn made grass silages ensiled in bunkers on commercial farms in South Wales during 1983-1985 were analysed to discern the effect of wilting and/or silage additives on fermentation. Silages were primarily made during late September and early October in fine weather from perennial ryegrass pasture which had not been grazed for 6 weeks. On average 13.9 ha of pasture was cut for silage. Analysis of 120 samples of grass showed it contained 176 g kg^?1 dry matter (DM) with (g kg^?1 DM) 215 protein, 240 modified acid detergent fibre (MADF), 78 water-soluble carbohydrate (WSC) and 95 ash. Most farmers attempted to pre-wilt grass for 1 day prior to ensilage and one quarter of them made unwilted silage. Silage making was usually completed within 6 days of starting to cut and was generally made with a precision-chop machine. Silage additives applied were (kg t^?1) formic acid (4·7), formic acid 4-formalin (5·5), sulphuric acid-+ formalin (5·0) sugars (14) and inoculants (0·65). Formic acid significantly reduced pH, and formic acid with or without formalin significantly reduced ammonia nitrogen (N) content of silages compared with other treatments. Protein contents of acid/formalin treated silages were significantly higher and MADF of acid with or without formalin treated silages were significantly lower than other treatments. Pre-wilting grass prior to ensilage did not significantly increase subsequent silage DM content and significantly increased the pH of non-additive treated silages. Unwilted silages treated with formic acid with or without formalin had a significantly lower ammonia-N content and higher residual WSC than other treatments. It is suggested that only formic acid application either alone or in conjunction with formalin to unwilted silage was successful in producing well preserved silages and that a grass WSC content of 17 g kg^?1 would be necessary to achieve this.  相似文献   

Comparison of the fermentation quality and nutritive value of sulphuric and formic acid-treated silages fed to beef cattle   总被引：2，自引：0，他引：2  

S. J. KENNEDY 《Grass and Forage Science》1990,45(1):17-28

Five experiments were carried out in the years 1980-1983 and 1986 to study the effect of treating grass at ensiling with sulphuric acid (850 g kg^?1) and formic acid (850 g kg^?1) additives alone, and in mixtures with or without formalin on the preservation of grass, in vivo digestibility in sheep, in-silo loss, intake and performance of finishing cattle. Primary growth grass was ensiled in experiments 1 (3–4 June 1980), 2 (12-15 June 1981) and 3 (31 May-2 June 1982), primary regrowth grass in experiment 4 (1-2 August 1983) and secondary regrowth grass in experiment 5 (7-10 October 1986). During the ensiling period within each experiment, approximately 60 t of unwilted, double-chopped, additive-treated or untreated grass was packed into covered concrete-walled 60-t capacity silos. The dry matter (DM) contents of the ensiled grass in experiments 1, 2 and 5 ranged from 155-180 g kg^?1 and were lower than those recorded in experiments 3 and 4 (214 g kg^?1). With the exception of grass ensiled in experiment 2, where water-soluble carbohydrate (WSC) contents were low, at 104 g kg^?1 DM, grass in all other experiments contained relatively high WSC contents ranging from 140-154 g kg^?1 DM. In experiments 1, 3 and 4 all silages were well-preserved. However, in experiment 2 the 450 g kg^?1 sulphuric acid-treated and formic acid-treated silages displayed significantly lower pH, buffering capacity (Bc) and ammonia nitrogen contents than the untreated silage. In experiment 5, the sulphuric acid-treated and formic acid-treated silages displayed significantly lower pH, Bc, ammonia nitrogen, butyrate and volatile fatty acid (VFA) contents than the untreated silage. Each of the silages was offered daily with various levels of a supplementary concentrate for approximately 70-d periods to twelve animals of mixed breed in experiments 1, 3, 4 and 5 and to fifteen animals in experiment 2. All animals weighed between 380-470 kg at the start of the experiments. In experiments 1, 2 and 3 there were no significant differences between silages for any of the intake or animal performance parameters. In experiment 4, cattle fed the formic acid-treated silage displayed significantly higher silage DM intakes and daily liveweight gains than those fed the sulphuric acid-treated and untreated silages; in experiment 5, cattle fed the formic acid-treated silage displayed significantly higher silage DM intakes than those fed the untreated silage. It was concluded that formic acid was a more effective silage additive than sulphuric acid. Increasing the level of supplementation significantly decreased silage DM intakes in cattle in experiments 3 and 4, and significantly increased daily liveweight gains and daily carcass in cattle in experiments 1, 3 and 4.  相似文献   

The composition of first-cut grass for ensilage in England and Wales from 1988 to 1991     

P. M. HAIGH 《Grass and Forage Science》1995,50(1):63-67

A total of 2225 samples of herbage, typical of that taken for first-cut silage on commercial farms in England and Wales, was taken between 1988 and 1991. Samples were analysed for dry matter (DM), water soluble carbohydrate (WSC), crude protein (CP) and metabolizable energy calculated from modified acid detergent fibre. The results were used to investigate the effect of weather conditions upon herbage quality for ensilage. In general, in areas of high rainfall, i.e. western areas, herbage DM and WSC were significantly lower than in eastern areas, whilst the reverse situation occurred with CP. Italian ryegrass (IRG) had a significantly higher WSC and lower CP than other grasses, whilst samples from permanent pasture (PP) had significantly lower DM and WSC, and significantly higher CP than other grasses. It was calculated that to produce a WSC concentration in the fresh grass of 37 g kg⁻¹, to produce well-fermented silage without additive use, IRG grown in the east would require a DM of 160 g kg⁻¹, In contrast PP grown in the west would require a DM content of 250 gkg⁻¹.  相似文献   

Proteolysis and amino acid degradation during ensilage of untreated or formic acid-treated lucerne and maize   总被引：3，自引：0，他引：3  

R. L. FAIRBAIRN  I. ALLI  L. E. PHILLIP† 《Grass and Forage Science》1992,47(4):382-390

Lucerne ( Medicago sativa ), field wilted to 330 g dry matter (DM) kg⁻¹, and whole-plant maize ( Zea mays ), 383 g DM kg⁻¹, were treated with formic acid (FA) at the rate of 4-5 1 t⁻¹ fresh forage. The effects of FA treatment on proteolysis and amino acid degradation was investigated at several intervals over a 90-d period of ensilage in laboratory silos. Formic acid treatment produced different patterns of pH decline during ensilage of the two forages, suggesting some degree of crop specificity in response to FA treatment. After 7 d of ensilage of lucerne there were increases of 81% in nonprotein nitrogen (NPN), and 104% in free amino acid nitrogen (FAA-N), but with FA-treated lucerne the increases were 39% and 31%, respectively. FA treatment resulted in a reduction in the concentration of most free amino acids in lucerne silage, with the exception of glutamic acid and serine; the branched chain amino acids as well as glycine, tyrosine and proline were significantly (P<0·05) reduced after 3 d of ensilage. By contrast, FA treatment of maize did not significantly ( p <0·05) affect the NPN content nor was there a consistent pattern in the changes in FAA-N. Formic acid treatment of maize did produce a significant reduction ( P <0·05) in the concentration of most individual free amino acids at 90 d of ensilage. Overall, FA treatment had a more predictable effect on proteolysis and amino acid degradation in ensiled lucerne than in ensiled whole-plant maize.  相似文献   

Enzymes as silage additives for grass–clover mixtures     

I. SELMER-OLSEN 《Grass and Forage Science》1994,49(3):305-315

Seven laboratory-scale experiments were carried out to study the effects of cellulases/hemicellulases on silage fermentation of herbage from mixed swards of timothy ( Phleum pratense ), meadow fescue ( Festuca pratensis ) and red clover ( Trifolium pretense ). Enzyme-treated silage (approximately 3500 HEC units kg⁻¹ grass) reached a low pH sooner, had lower end pH, contained less NH₃-N and more lactic acid than did the untreated silage ( P < 0·05). Applied with an inoculant, these effects were even stronger. With easily ensiled crops (experiments 1, 2, 5 and 6) preservation was first of all improved by inoculation of lactic acid bacteria; however, for the low-sugar crops (experiments 3, 4 and 7) enzyme treatment was more significant. The enzymes derived from Aspergillus spp. gave more acetic acid than the enzymes from Trichoderma reesei. The Trichoderma enzymes liberated 4·8 g WSC kg⁻¹ FM gamma-irradiated grass during 60 d at pH 4·3 ( P < 0·05). On average, for all silages enzyme treatment increased the sum of residual sugar and fermentation products by 3·7 g kg⁻¹ FM (21 g kg⁻¹ DM) compared with the silages not treated with enzymes ( P < 0·001). Enzyme treatment increased the instantly degradable part of the feed, but total in sacco and in vitro digestibilities were not affected.  相似文献   

A comparison of unwilted and wilted grass silages offered to beef cattle without and with monensin sodium     

R. W. J. STEEN 《Grass and Forage Science》1984,39(1):35-41

A randomized block experiment was conducted to compare unwilted and wilted grass silages and the effects of the feed additive monensin sodium on the silage intake and performance of finishing beef cattle. Two regrowths from a predominantly perennial ryegrass (cv. S24) sward were ensiled either without wilting or after field wilting for 3 d (dry matter (DM) concentrations 161 and 266 g kg⁻¹ respectively). Both silages were treated with formic acid (2·6 and 30 litre t⁻¹ respectively) and were well preserved. The silages were offered ad libitum to forty-eight Charolais-cross cattle (thirty-two steers and sixteen heifers, mean initial live weight 351 kg) for 145 d. All animals received 2·2 kg concentrates per head daily and half of those on each silage treatment received in addition 200 mg monensin sodium per head daily. Silage DM intake was 5.04, 504. 5·48, 5·63 ± 0.134 kg d^−l; fasted liveweight gain was 0·69, 0·77. 0·64 and 0·73 ± 0.033 kg d^−l and carcass gain was 0·47, 0·50, 0·40 and 0·45 ± 0·020 kg d⁻¹ for the unwilted silage without and with monensin and the wilted silage without and with monensin respectively. It is concluded that wilting grass of low DM concentration for 3 d prior to ensiling reduced the performance of finishing beef cattle below that obtained from well-preserved unwilted silage in spite of a higher DM intake being achieved with the wilted silage. The inclusion of monensin sodium in a silage-based diet increased performance without significantly affecting feed intake.  相似文献   

The effect of inoculation with Lactobacillus plantarum on fermentation in laboratory silos of herbage low in water-soluble carbohydrate   总被引：1，自引：1，他引：1  

J. A. ROOKE  ALISON J. BORMAN  D. G. ARMSTRONG 《Grass and Forage Science》1990,45(2):143-152

In experiment 1, a mixture of perennial ryegrass and white clover (176 g DM kg^?1) was ensiled with no addition, or with the application of Lactobacillus plantarum, 3 × 10⁶(g fresh weight of herbage)^?1 or enterobacteria, 3 × 10⁶ (g fresh weight of herbage)^?1 or both of these inoculants. Silos were incubated at either 18°C or 26°C for 7 d and sealing of half of the silos was delayed for 48 h. In experiment 2, cocksfoot (247 g DM kg^?1) was ensiled with no additive, with the application of L. plantarum, 4 × 10⁶ (g fresh weight of herbage)^?1 enterobacteria, 4 × 10⁶ (g fresh weight of herbage)^?1 or ammonium nitrate, 5 g (kg herbage)^?1. Silos were incubated at 18°C and sealing was delayed for 48 h. In neither experiment were the untreated silages of satisfactory quality after 35 d ensilage, having pH's of 4·3 and 6·4. and ammonia-N concentrations of 139 and 209 g N (kg total N)^?1 in experiments 1 and 2 respectively. The poorer fermentation in experiment 2 reflected the lower water-soluble carbohydrate (WSC) content of the ensiled herbage (136 and 53 g WSC (kg DM)^?1 in experiments 1 and 2 respectively). Inoculation with L. plantarum significantly reduced silage pH and the concentrations of ammonia-N (experiment 1 only) and ethanol and increased lactic acid concentrations. Inoculation with enterobacteria had only minor effects in each experiment. In experiment 1, incubation at 26°C increased the rate of fermentation in the silos as pH was significantly lower after 7d incubation at 26°C than at 18°C, but this effect had largely disappeared after 35 d incubation. When sealing of the silos was delayed for 48 h, the resulting silages had significantly higher pH and ammonia-N contents than silages from silos that had been sealed immediately after filling. In experiment 2, addition of ammonium nitrate significantly reduced the concentrations of ethanol and acetic acid in silages opened after 7 d. After 35 d the concentrations of ethanol were significantly reduced and those of ammonia-N increased by the addition of ammonium nitrate. There were marked increases in silage pH between 7 and 35 d ensilage and in the concentrations of ammonia-N and acetic acid in the silages that had been inoculated with ammonium nitrate at ensilage.  相似文献   

Enzymes as silage additives. 1. Silage quality, digestion, digestibility and performance in growing cattle   总被引：5，自引：0，他引：5  

J. L. JACOBS  A. B. McALLAN 《Grass and Forage Science》1991,46(1):63-73

Four grass silages were made from perennial ryegrass ensiled after a 1h wilt in 2-t silos without additive application, with application of formic acid or with one of two enzyme mixtures of hemicellulases and cellulases (enzyme 1 and enzyme 2). Effluent losses were monitored over the ensiling period (130 d).
Analyses of the silage showed that formic acid-treated silage had lower concentrations of lactic acid than the other silages. Both enzyme-treated silages had lower levels of cellulose, acid detergent fibre (ADF) and neutral-detergent fibre (NDF) than the untreated and formic acid treated silages. Effluent production was highest with enzyme-treated silages.
The silages were subsequently fed to growing steers equipped with rumen cannulae and T-piece duodenal cannulae. Apparent whole-tract digestibilities of organic matter constituents were significantly lower ( P < 0·05) with both enzyme-treated silages (untreated; 0·736, formic acid; 0·722, enzyme 1; 0·694, enzyme 2; 0·703). Both untreated and enzyme 2-treated silages sustained higher nitrogen digestibilities (g g⁻¹ intake) (untreated; 0·675, formic acid; 0·636, enzyme 1; 0·630, enzyme 2; 0·662) and N retentions (g d⁻¹) untreated; 16·0, formic acid; 14·0, enzyme 1; 11·6, enzyme 2; 16·6), but none of these differences was significant. When formic acid-treated silage was offered, there was a greater amount of organic matter apparently digested in the rumen (ADOMR). Non-ammonia nitrogen and microbial nitrogen flows at the duodenum were similar on all diets. The efficiency of microbial protein synthesis was highest with enzyme 2-treated silage and lowest with formic acid-treated silage (untreated, 35·4; formic acid, 25·2; enzyme 1, 30·4; enzyme 2, 39·4), but none of these differences were significant.  相似文献   

Determination of microbial protein in perennial ryegrass silage   总被引：1，自引：0，他引：1  

F. Driehuis  & P. G. van Wikselaar 《Grass and Forage Science》2001,56(1):75-83

The microbial matter fraction was determined in perennial ryegrass silages of different dry-matter (DM) contents, ensiled with or without Lactobacillus plantarum . ¹⁵N-Leucine and the bacterial cell wall constituent diaminopimelic acid (DAPA) were used as markers for microbial-N. Perennial ryegrass crops with DM contents of 202, 280 or 366 g kg⁻¹ fresh weight were ensiled in laboratory-scale silos and stored for 3 to 4 months. At different times after ensiling, silages were analysed and microbial fractions were isolated. Microbial-N concentration determined with ¹⁵N-leucine reached a maximum during the first week of ensilage. It remained unchanged thereafter, except in silage with a DM content of 280 g kg⁻¹ in which it decreased ( P  < 0·01) by 32% during storage. After 3 to 4 months ensilage, microbial-N concentration varied from ≈0·3 to ≈1·7 g kg⁻¹ DM. A negative relationship was observed between microbial-N concentration and silage DM content. Inoculation resulted in an approximately twofold increase ( P  < 0·001) in microbial-N concentration. Microbial-N concentrations determined with DAPA were 1·14–2·07 times higher than those determined with ¹⁵N-leucine. However, 19–35% of the DAPA in silage occurred in a soluble form, indicating that this fraction of DAPA was not associated with intact bacteria.  相似文献   

Quality of grass silage infected with spores of Clostridium tyrobutyricum     

CHRI RAMMER 《Grass and Forage Science》1996,51(1):88-95

The influence of adding clostridial spores to silage was studied in 2 successive years. Direct-cut, precision-chopped herbages were infected with spores of Clostridium tyrobutyricum in doses varying from 0 to 10⁶ g^−l fresh material (FM), and ensiled in experimental silos. The material was treated with or without 4 ml formic acid (85%) kg⁻¹ FM. Silo contents were studied after 4.20 (or 50) and 100 days of ensiling.
Contents of dry matter, water-soluble carbohydrate, nitrate as well as buffering capacity of FM varied between the 2 years at harvest, whereas the numbers of clostridial spores were about the same (< 10² g⁻¹).
Infecting the crop with clostridial spores negatively affected the quality of silage in the first year. After 50 days spore numbers in the silages had increased by 40 × 10³ to 150 × 10³ times in the infected silage, compared with 5 × 10³ in the uninfected silage. No effect was seen in the second year.
Silage with high spore counts showed no residual nitrate content. Formic acid improved silage quality from a chemical point of view but had no anti-clostridial effect. Ensilability of a crop seems to be more important than the initial count of Clostridia for a successful ensiling process.  相似文献