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1.
A total of 144 male crossbred calves were allocated to four management treatments (bulls; steers; bulls implanted with zeranol at 100 d of age and re-implanted at 69, 93 and 56 d thereafter; bulls implanted with zeranol at 168 d of age and re-implanted at 93 and 56 d thereafter), and two pre-slaughter shipping treatments (minimum pre-slaughter stress with cattle shipped and slaughtered within 4 h of leaving the feedlot pen; moderate pre-slaughter stress with cattle mixed, trucked 160 km and slaughtered up to 24 h of leaving the feedlot pen) in a 4 X 2 factorial arrangement. Management treatment had no significant effect on carcass pH (45 min), carcass muscle temperature (45 min), or peak shear-force of cooked longissimus muscle. Steers had significantly lower dressing percentage, warm-carcass weight, hide weight and carcass-lean content, but higher marbling score, fat thickness and intramuscular-fat content than all treatments with bulls. Minimum pre-slaughter stress resulted in significantly lower dressing percentage, warm-carcass weight, and carcass pH (45 min), but generally had no effect on carcass tissue-yield measurements compared with the moderate stress treatment. Implanted bulls produced carcasses with significantly darker meat, higher 24-h pH and lower meat expressible juice than bulls and castrates for the moderate pre-slaughter stress treatment. These results provide evidence that zeranol implantation in bulls had a minor influence on carcass characteristics, and did not reduce the incidence of dark-cutting carcasses in young bulls subjected to moderate pre-slaughter shipping stress.  相似文献   

2.
The aim of this study was to evaluate the impact of the slaughter process on the Campylobacter (C.) coli prevalence on pig carcasses and finally pork. To detect C. spp., faecal samples, organ samples and surfaces of slaughter pigs were sampled. Additionally, various abattoir surfaces (n=208) and 227 pork and minced meat samples were included in our study.Whereas a high C. spp. prevalence (64.0%) was detectable in the faeces of slaughter pigs (all isolates were identified as C. coli), low detection rates were observed on pig carcasses after the slaughter process before the chilling period (21.1%).The impact of chilling reduced the detection rate of C spp. on pig carcasses even further to 0.8%. Only C. jejuni strains were isolated after the chilling process. Chilling and the associated drying of the skin are responsible for that massive reduction of C. spp prevalence. Significantly more C. spp. were isolated from livers compared to the corresponding carcasses. Only 5 out of 208 swab samples from different surfaces of the abattoir were C. coli positive. Bacteriological investigation could not detect any C. spp. strains from pork and minced pork meat.The low detection rates at the end of the slaughter and processing line indicate that pork may only play a minor role in the transmission of C. coli infections to humans. By genotyping C. coli-isolates from selected animals we were able to demonstrate three possible ways of contamination of the slaughter carcass surface. Genetically highly related strains were detectable on carcass surfaces of consecutively slaughtered animals. Faecal isolates and isolates from the carcass surface showed occasional high similarities. C. coli-genotypes from tonsils and genotypes from the corresponding slaughter carcasses formed a close cluster.  相似文献   

3.
试验旨在研究商品猪血液中皮质醇含量与宰后的胴体性状和肉质表观指标之间的相关性并探讨其内在联系。在具有秋季典型气温时间(2012年8月20日),选取120头商品猪,猪只运输时间约为2h,运输到屠宰场后休息1.5h后屠宰,取血液样品测定皮质醇含量,取背最长肌测定肉质指标,并测定猪只的胴体重和背膘厚。根据测定得到的皮质醇浓度,将猪只分成三个组,低皮质醇组(〈120ng/mL)、中皮质醇组(120~130ng/mL)和高皮质醇组(〉130ng/mL),比较三组之间肉质和胴体指标之间的差异。结果表明,在肉质指标方面,除中皮质醇组猪只的24h的电导率有升高的趋势(P=0.08)外,其余均无显著差异;在胴体性状方面,高皮质醇组胴体重极显著高于(P〈0.01)低皮质醇组,高皮质醇组背膘厚显著高于(P〈0.05)低皮质醇组,与中浓度皮质醇组差异均不显著。猪只血液中皮质醇含量与胴体重和背膘厚度都存在着极显著的正相关,相关系数分别为0.349和0.274。各肉质指标之间也存在相关性,其中PH与电导率为极显著的负相关,pH与肉色的L值呈极显著的负相关,45min时测定的肉色L值与滴水损失相关系数为0.234,猪只胴体重和背膘厚度呈极显著的正相关关系,相关系数为0.596。结果提示,无法通过猪只血浆皮质醇浓度的高低来评估宰后猪肉品质,但一定程度内较高的皮质醇浓度会有利于个体增重和背部脂肪的沉积。  相似文献   

4.
Abstract

The aim of our study was to investigate the effect of rearing system (mixed or single-sex) on performance and carcass quality of entire male and female pigs slaughtered at around 90 or 115 kg live weight. We also studied the combined effect of live weight and dietary supplement of raw potato starch (RPS) on technological meat quality for both sexes, and boar taint compounds in entire male pigs. Furthermore, we investigated pre-slaughter routines (pen-wise or mixing with unfamiliar pigs) and sexual maturity. A total of 408 growing/finishing pigs were included in this two-year study. Performance did not differ significantly between entire male and female pigs irrespective of rearing system, except for daily weight gain in the interval from 90 kg to slaughter. Mixed pigs had significantly higher daily weight gain in this interval than single-sex pigs. Entire male pigs had higher carcass lean meat content than female pigs (1.6 percentage units). Technological meat quality differed only slightly between the sexes. Females reared in mixed pens were sexually more mature than female pigs reared in single-sex pens, and mature females had higher growth rate but lower meat content in carcasses than immature females. In contrast, female contact did not influence sexual maturity of entire male pigs. Mixing entire male pigs with unfamiliar pigs at the abattoir resulted in more damaged skin compared with pen-wise keeping whereas female pigs had almost no visible skin damage. We therefore recommend that entire male pigs are not mixed with unfamiliar pigs during transport and at the abattoir, because of fighting and subsequent skin damage. The levels of skatole in fat were significantly lower in high-weight entire male pigs fed RPS compared to high-weight pigs without RPS. Androstenone levels in fat were high in all groups.  相似文献   

5.
选用不同致晕、放血法及烫毛方法对140头杜长大和杜大长三元杂商品猪进行屠宰,测定其肌肉的肉色、pH值、剪切力、失水率等指标。结果表明:在其他条件相同的情况下,烫毛水温为60℃时,烫毛6min,胴体质量最佳。电击致晕生猪,采用悬挂放血,有出现PSE猪肉的倾向;而CO2致晕生猪,采用水平放血或悬挂放血,肉质指标差异不显著。较好的组合是:电击致晕生猪,采用水平放血;而CO2致晕生猪,采用悬挂放血较好。  相似文献   

6.
The study hypothesis was that pre-slaughter handling is not conducted in such a way that pigs can be considered humanely slaughtered, nor is it conducive to promoting those properties of fresh meat that could enhance pork quality. The 1st phase of the investigation was aimed at confirming the present compliance level within the prescribed norms. To this end the importance of stunning induction requirements for effective electrical stunning, the welfare implications relating to the pre-slaughter handling of pigs and the stunning and sticking techniques were investigated. Pre-slaughter welfare of the pigs at the abattoir was found to be influenced at the outset by the origin and type of pig slaughtered, the daily throughput range of pigs and the type of abattoir involved. A disappointing pre-arrival aspect was that deficiencies in road motor vehicles were observed while off-loading pigs. Through poor design or lack of maintenance, another factor that hampered free movement of pigs was the off-loading facilities. The nature of animal behaviour in the pens, in the passages and when going into the stunning area was directly related to the pig handling efficiency and to the nature and extent of design and maintenance problems with equipment. None of the abattoirs had a well designed in-feed to a well planned stunning area/facility, and a very high level of pig pre-slaughter stress prevailed. The optimum position of the electrodes is virtually impossible to attain under practical conditions and the practice of repeat application of electrical stunning is common. Of the total sample size (n = 1175), 34% were difficult to shackle, 90% of these being from the abattoirs tending to slaughter more routinely a wide range of different sized pigs. In this survey 149 pigs (13%) required more than a single sticking attempt to bleed out properly, considerably higher than in other published reports.  相似文献   

7.

Background

Tail biting is a common welfare problem in pig production and in addition to being a sign of underlying welfare problems, tail biting reduces welfare in itself. The aim of this study was to evaluate the effects of tail biting on different pre and post mortem indicators of stress in slaughter pigs and on carcass and meat characteristics. A total of 12 tail bitten (TB) and 13 control (C) pigs from a farm with a long-term tail biting problem were selected for salivary cortisol analyses before and after transport to the slaughterhouse. After stunning, samples were taken for the analysis of serum cortisol, blood lactate, intestinal heat shock protein 70 (HSP70), and meat quality characteristics. In addition, body temperature immediately after and muscle temperature 35 min after stunning were measured, as well as lean meat percentage and carcass weight.

Results

TB pigs showed a lower cortisol response to the transport-induced stress than C pigs and also had a lower serum cortisol concentration after stunning. HSP70 content in the small intestine was higher in the TB pigs than in C pigs. TB pigs had a considerably lower carcass weight therefore produced a lower total amount of lean meat per carcass than C pigs.

Conclusions

This study suggests that prolonged or repeated stress in the form of tail biting causes a blunted stress response, possibly a sign of hypocortisolism. In addition, it underlines the importance of reducing tail biting, both from an animal welfare and an economic point-of-view.  相似文献   

8.
Crossbred pigs (n = 192) from Piétrain x Large White sires mated to Landrace x Large White dams, with a mean BW of 75 +/- 1.3 kg, were used to investigate the effects of gender and slaughter weight (SW) on growth performance, carcass characteristics, and meat quality. Pens of pigs (eight pigs/pen) were assigned randomly to one of six treatments arranged in a 2 x 3 factorial design with two genders (barrows or gilts) and three SW (116, 124, or 133 kg). Each treatment was replicated four times. Over the entire trial, barrows had higher (P < 0.001) ADFI (as-fed basis) and ADG than gilts; however, gilts had higher (P < 0.05) gain-to-feed ratios (G:F) than barrows. Barrows had lower (P < 0.01) dressing percents than gilts and produced fatter (P < 0.001) carcasses that had lower trimmed shoulder (P < 0.10) and ham (P < 0.001) yields than gilts. There was a trend for the semimembranosus muscle (SM) from barrows to have a higher (P < 0.10) 45-min pH than that of gilts, but 24-h pH was 0.11 pH unit higher (P < 0.01) in the SM of barrows than gilts. Gender had no (P > 0.10) effect on the moisture and lipid content of the longissimus muscle (LM), nor did gender affect (P > 0.10) LM color, myoglobin content, or thaw loss percentage. However, the LM from barrows had lower (P < 0.05) cooking loss percentages and tended to have lower (P < 0.10) shear force values than the LM from gilts. Pigs slaughtered at 116 kg had higher (P < 0.05) ADG than pigs slaughtered at 124 and 133 kg. Daily feed intake (as-fed basis) was not (P > 0.10) different among SW; however, pigs slaughtered at 116 and 124 kg had higher (P < 0.001) G:F than those slaughtered at 133 kg. Dressing percent, backfat depth, carcass length, and ham and shoulder weights increased (P < 0.001) as SW increased from 116 to 133 kg. The initial (45-min) pH of the SM from pigs slaughtered at 133 kg was higher (P < 0.05) than from pigs slaughtered at 116 or 124 kg; however, 24-h pH was not (P > 0.10) affected by SW. The LM from pigs slaughtered at 133 kg was darker (lower L* values; P < 0.001), redder (higher a* value; P < 0.01), and had more (P < 0.001) myoglobin than the LM of pigs slaughtered at 116 and 124 kg. Barrows and gilts of this particular crossbreed can be used to produce acceptable quality fresh pork when slaughtered at 116 kg; however, increasing SW to 124 kg, or more, decreased live pig performance and carcass leanness without any additional benefits to pork quality attributes.  相似文献   

9.
旨在研究不同脱脂米糠水平日粮对苏淮猪胴体性状及肉品质的影响。选取35头初始体重为(62.9±0.8)kg的健康纯种苏淮阉公猪,随机分为5组,每组7个重复,每个重复1头猪,使用奥斯本种猪生产性能测定系统(OTSS)饲喂。对照组(CTRL)饲喂不含脱脂米糠的基础日粮,试验Ⅰ~Ⅳ组分别饲喂7%、14%、21%和28%脱脂米糠的试验日粮,5组日粮的中性洗涤纤维(NDF)水平分别为8.89%、11.80%、12.93%、14.35%和17.94%,各组日粮除纤维水平不同外,其他营养成分基本一致。试验预饲期10 d,所有猪饲喂基础日粮;正式期28 d,分别饲喂基础日粮和试验日粮。试验结束屠宰全部试验猪,测定其胴体性状(胴体重、屠宰率、胴体直长、胴体斜长、眼肌面积、平均背膘厚和皮厚);采集肉样用于测定肉质性状(滴水损失、剪切力、熟肉率、肌内脂肪含量、pH和肉色);采集背最长肌样品用于猪滴水损失主效基因磷酸化酶激酶γ1(phosphorylase kinase gamma1,PHKG1)表达分析。结果表明:1)日粮脱脂米糠水平对苏淮猪的胴体重、屠宰率、胴体直长、胴体斜长、眼肌面积、平均背膘厚和皮厚等胴体指标没有显著影响。2)随脱脂米糠水平的提高,苏淮猪背最长肌的滴水损失呈先降低后上升的二次曲线变化(P<0.05),猪肉的剪切力线性降低(P<0.05);熟肉率、pH24 h随脱脂米糠水平的增加呈线性增加的趋势(P=0.061,P=0.068);日粮脱脂米糠水平的增加有降低L24 h*的趋势(线性,P=0.085),脱脂米糠水平对其它肉质指标均无显著影响。3)PHKG1基因的相对表达量随脱脂米糠水平的增加趋于二次方升高(P=0.085)。综上所述,日粮脱脂米糠水平对苏淮育肥猪的胴体性状无显著影响,但在日粮中适度添加脱脂米糠可降低苏淮猪猪肉的滴水损失及剪切力,改善苏淮猪猪肉品质,但其背后的机制还有待进一步深入研究。  相似文献   

10.
Pale, soft, exudative (PSE) pork occurs, for the most part, from environmental stress on the pig. Amino acid intake may be related to stress susceptibility through hormone and neurotransmitter induction. Two experiments were conducted to determine whether supplementation of 5 g of tryptophan (TRP) or 10 g of tyrosine (TYR) per kilogram of a 14% CP diet would alter the response of pigs to stress as measured by hypothalamic neurotransmitter concentrations and incidence of PSE. Twenty-four (Exp. 1) and 36 (Exp. 2) 92-kg pigs were offered one of three diets: control, TRP-, or TYR-supplemented for 5 d before slaughter. Dietary TRP or TYR supplementation in Exp. 1. doubled (P less than .05) plasma TRP and TYR concentrations, respectively, and increased (P less than .05) 5-hydroxytryptamine, dihydroxyphenyl ethylamine, dihydroxyphenyl acetic acid, and homovanillic acid concentrations in the hypothalamus. Pigs that exhibited stress at slaughter had lower (P less than .05) hypothalamic concentrations of epinephrine, norepinephrine, and 5-hydroxytryptamine. In Exp. 2, pigs were trucked 55 km to a commercial meat packing facility and slaughtered without a rest period. This handling procedure was designed to invoke a high incidence of PSE pork and thus be a strong test of treatments. Supplemental dietary amino acids seemed to alter the frequency distribution of the severity of PSE pork. These data indicate that dietary manipulation of amino acid precursors of neurotransmitters may offer a practical means of reducing stress response in swine.  相似文献   

11.
In this study the effects of barren vs enriched housing conditions of pigs on their behavior during the lairage period (2-h holding period before slaughter), carcass characteristics, postmortem muscle metabolism, and meat quality were studied. The barren housing system was defined by common intensive housing conditions (i.e., with slatted floors and recommended space allowances), whereas the enriched environment incorporated extra space and straw for manipulation. Salivary cortisol concentrations were measured before transport and at the end of the lairage period. During the lairage period the percentage of time spent walking and fighting by the pigs was registered. Carcass characteristics such as weight, meat percentage, and backfat thickness were determined. At 5 min, 45 min, 4 h, and 24 h postmortem, pH, temperature, and lactate concentrations were determined in the longissimus lumborum (LL) and biceps femoris (BF) muscles. Capillarization of the muscle, mean muscle fiber area, and color and drip loss after 2 and 5 d of storage were determined for both muscle types. Pigs from the barren environment had a significantly higher increase in cortisol from farm to slaughter, but no differences in behavior were observed during the lairage period. Carcass characteristics did not differ between pigs from barren and those from enriched housing conditions. Postmortem lactate formation was significantly lower in LL muscles of enriched pigs at 4 and 24 h postmortem. Capillary density and mean muscle fiber area did not differ between the groups of pigs. The percentage of drip loss at 2 and 5 d after storage of LL muscle samples from enriched-housed pigs was significantly lower than that of the barren-housed pigs. Similar tendencies were found for the BF muscle from pigs kept in an enriched environment, but these were not statistically significant. The housing system did not affect meat color. It is concluded that on-farm improvement of animal welfare by environmental enrichment can also lead to beneficial economic effects after slaughter by improving the water-holding capacity of pork.  相似文献   

12.
The study aimed to reduce cross-contamination between finishers from Salmonella-positive and Salmonella-negative herds during transport, lairage, and slaughter, thereby reducing the prevalence of Salmonella Typhimurium on slaughter carcasses. In Phase 1 of the study, pigs from Salmonella-negative herds were kept in lairage for 2-4 hours either in clean pens (intervention group) or pens contaminated with Salmonella-infected faeces (control group). All pigs were slaughtered on the same slaughterline, and carcass swabs 24 hours after slaughter revealed a low degree of cross-contamination in the pens: there was no difference in Salmonella-positive carcasses between intervention (1.7%) and control groups (0.8%). In Phase 2, control pigs from Salmonella-negative herds were mixed with pigs from Salmonella-positive herds during lairage for 2-4 hours, while the intervention group still consisted of pigs from Salmonella-negative herds. All pigs were slaughtered on the same line: first intervention, then control. Carcass swabs taken 24 hours after slaughter failed to show a reduction in Salmonella-positive carcasses in the intervention group (4.5%) compared with the originally Salmonella-negative pigs in the control group (3.6%). In pigs from Salmonella-positive herds the occurrence of Salmonella was substantially higher at 10.4%. When the results were corrected for 6 carcass samples found positive with S. Heidelberg on the same day, which was attributed to a transient hygiene failure, only 2.2% of the carcasses in the intervention group were Salmonella-positive. We conclude that even though cross-contamination occurs in the abattoir pens, its importance on the slaughter line may be greater. However, the final results of this study should be awaited to conclude whether separate slaughter of pigs from Salmonella-positive and Salmonella-negative herds should be recommended.  相似文献   

13.
We examined the effects of vitamin and mineral supplementation of the finishing diet on growth and accelerated chilling of carcasses on carcass and muscle traits of halothane gene carrier and noncarrier pigs. Barrows and gilts that were either monomutants (MON, n = 49) or noncarriers (NON, n = 28) of the halothane gene were fed a standard finishing diet until they reached 86 kg. They then were randomly assigned to one of four finishing diets formulated to contain 11 IU/kg vitamin E (0), 311 IU/kg vitamin E plus additional vitamins and minerals (300), 611 IU/kg vitamin E plus additional vitamins and minerals (600), or 911 IU/kg vitamin E plus additional vitamins and minerals (900) until they were slaughtered (118 kg). Alternating carcass sides were assigned either a normal chilling procedure (NC, 4 degrees C for 24 h) or an accelerated chilling procedure (AC, -20 degrees C for 1.5 h and then 4 degrees C for 22.5 h). Supplementing vitamin E in the finishing diet increased (P < 0.05) the concentration of vitamin E in the longissimus muscle. Supplementing vitamin E in the diets of MON pigs did not affect color, firmness, or cooking losses of loins or color and firmness of hams. For the NON genotype, increasing the level of vitamin E in the diet decreased (P < 0.05) the percentage of PSE loins and hams. Color and firmness scores of the gluteus medius and longissimus muscles were improved 0.4 unit (P < 0.005) by AC compared with NC of carcasses. Loin chop juiciness and flavor were improved (P < 0.05) in the MON genotype for AC compared to NC. Accelerated chilling reduced (P < 0.05) the percentage of PSE loins from 38 to 17% and PSE hams from 32 to 10% for the MON genotype, but percentage of PSE was not affected (P > 0.05) by chilling treatment for the NON genotype. No interaction between diet and chill treatments existed for muscle quality traits (P > 0.05). Supplementing finishing diets of NON pigs with at least 600 IU/kg vitamin E, in addition to other vitamins and minerals, or accelerated chilling of MON carcasses can reduce the incidence of PSE pork.  相似文献   

14.
One hundred seventy-seven pigs were used to determine the interaction effects of fasting and length of transport prior to harvest on pork muscle quality. The study design was a 2 x 2 x 3 factorial, which involved two genetic sources, fasting (F) or no fasting (N) of pigs 48-h prior to harvest, and three transport times (0.5, 2.5, or 8.0 h) on a semitrailer to the packing plant. Genetic source was a significant source of variation (P < 0.05) for most composition and muscle quality variables. Fasting reduced hot carcass weight 3.6% (P < 0.05), but length of transport did not affect hot carcass weight (P > 0.05). There were no differences (P > 0.05) in percent lean among fasting and transport treatments. Fasted pigs had higher longissimus dorsi (LD) ultimate pH (pHu), darker lean color, higher marbling score and lower 7-d purge loss, 24-h drip loss, and cooking loss (P < 0.05) than nonfasted pigs. Meat from pigs that were transported 8.0 h had lower glycolytic potential (GP), higher LD and semimembranosus (SM) pHu, darker lean color, and lower L*, 7-d purge loss, 24-h drip loss, cooking loss, and shear force values than meat from pigs transported 0.5 h (P < 0.05). Meat from pigs transported 2.5 h had higher LD and SM pHu and lower L*, 7-d purge loss, 24-h drip loss, and cooking loss than meat from pigs transported 0.5 h (P < 0.05). Meat from pigs transported 8.0 h had higher LD pHu and color scores and lower L* and cooking loss than meat from pigs transported 2.5 h (P < 0.05). The fasting x transport interaction was significant for SM pHu, L*, color score, and drip loss. Fasting improved SM pHu, L*, color score, and drip loss for pigs that were transported 0.5 h (P < 0.05), but when pigs were transported for 2.5 h or 8.0 h, fasting had little or no effect on these muscle quality traits. Fasting lowered GP and increased LD pHu for pigs from the genetic source with the higher initial pork quality (P < 0.05), while fasting had no effect on pork quality for pigs from the genetic source with the lower initial pork quality (P > 0.05). Longer transport times resulted in lower GP and higher LD pHu regardless of genetic source. Fasting and length of transport each had positive effects on pork quality, but length of transport effects was greater in magnitude. When pigs were transported for 0.5 h, fasting for 48 h prior to harvest improved pork quality, but when pigs were transported 2.5 or 8.0 h, fasting had little effect on pork quality.  相似文献   

15.
The aim of this study was to compare the prevalence of Salmonella serotypes at two different sites on pig carcasses from herds classified as high-risk or low-risk and to elucidate the relationship between carcass contamination levels and serological status. Caecal samples and carcass surface swabs were cultured for Salmonella from a total of 210 pigs from low risk herds (< 19% of pigs in herd Salmonella seropositive) and 209 pigs from high risk herds (> 32% of pigs in herd Salmonella seropositive) in three abattoirs. Meat juice samples were collected for analysis by ELISA. The prevalence of Salmonella in the caecal contents of "low-risk" pigs was 10%, which was significantly lower than the 19% prevalence in "high-risk" pigs (p < 0.01). The corresponding figures for skin samples collected immediately post-evisceration were 2% and 12%. The predominant Salmonella serotype in the caecal contents of both the low-risk and high-risk pigs was Salmonella Typhimurium. Salmonella Kentucky and Salmonella Derby were the most frequent isolates from the carcass surface swabs of low- and high-risk pigs respectively. There was a positive association between seropositivity of pigs from high-risk herds and caecal carriage (p < 0.05). Results showed that herd categorisation based on serological results was useful in predicting Salmonella isolation rates from caecal samples and surface swabs of slaughtered pigs.  相似文献   

16.
Occurrences of thermophlic Campylobacter in pigs and pig carcasses was investigated in a cross-sectional study that was carried out in three selected slaughter slabs in Morogoro municipality, Tanzania. Before sampling, the slab hygiene, slaughter, carcass dressing, and meat handling was assessed. Fecal samples were collected from 66 slaughter pigs at the kill floor. After slaughter, a 100-cm2 area on medial surface of the thigh muscles of dressed carcasses was sampled using sterile cotton swabs. Thereafter, the jejunal, cecal, and colon contents were also sampled. The samples were subjected to standard bacteriological examination using Skirrows protocol. In all slaughter slabs visited, it was found that pig slaughter, dressing, and meat handling was done on the ground under unhygienic condition. All the slaughter slab environment were dirty and had neither tap water or drainage systems. Thermophilic Campylobacter prevalence in slaughtered pig was 66.7% while contamination rate of dressed carcasses was 10.6%. Of the Campylobacter-positive carcasses, five (12.2%) were from the animals which were also positive to Campylobacter. The isolation rate of Campylobacter in the cecum was higher (34.8%) compared to the small intestines (28.8%) and colon (16.7%) although the difference was not statistically significant (P > 0.05). Campylobacter jejuni was the most prevalent species as it constituted 74% of all isolates, while Campylobacter coli was isolated at 26%. This suggests possible risks of infection to people through consumption of contaminated pork or through contact with infected pigs. Cecum was found to be the major part of intestine highly colonized by Campylobacter.  相似文献   

17.
The present study investigated the effect of outdoor rearing and genetic background on behaviour and meat quality traits in heavyweight pigs. Large White and Duroc-sired pigs were reared in groups of 3 castrated males and 3 females, conventionally or in fields, from April to October, in a study with two replicates. Compared to indoor pigs, outdoor pigs were more active and showed a larger range of behaviour suggesting improved welfare from a behavioural point of view. During 5 h of isolation, outdoor pigs were less active and had lower heart rates, and in another test, they reacted less to a non-familiar object (traffic cone). Pigs were slaughtered at 150 kg live weight, half of each treatment group after mixing, short transport, and overnight lairage and half immediately following short transport. Outdoor pigs were less aggressive during pre-slaughter mixing. Ante- and post-mortem glycogen content of the Longissimus lumborum (LL), Semimembranonus (SM) and Semispinalis capitis (SC) muscles depended on slaughter conditions, sire breed, rearing conditions, gender and year of experimentation, sometimes influencing ultimate pH. Effects of rearing on muscle glycogen content and post-mortem pH were stronger during the second year of rearing. Effect of slaughter conditions on glycogen content and ultimate pH depended on fighting levels during pre-slaughter mixing. Outdoor rearing increased muscle redness. Drip and cooking loss were higher in Large White than Duroc-sired pigs. Drip and cooking loss were correlated with early post-mortem and ultimate pH. Thawing loss was correlated with early post-mortem temperature and ultimate pH. Finally, reactivity to isolation had a predictive value as pigs more active during isolation had less skin damage due to fighting during pre-slaughter mixing. In conclusion, despite large effects of year of experimentation, outdoor rearing, sire breed, and slaughter conditions influenced behaviour and muscle characteristics.  相似文献   

18.
The purpose of this study was to determine the effects of short-term, preslaughter stress on physiological responses and meat quality in goats of different age groups. The goats (n = 28) were classified into young (6 to 12 mo of age) and old (24 to 30 mo of age) groups, feed deprived overnight, and slaughtered at three different times (replicates). On each slaughter day, goats were either subjected to a 2-h transportation stressor (TS) or remained unstressed in holding pens (NS) before slaughter. Blood samples were collected via jugular venipuncture from TS and NS goats at 2, 1, and 0 h before slaughter. Muscle glycogen and pH were measured on samples from longissimus muscle (LM) collected at 15 min and 24 h postmortem, and instrumental measures of meat color were obtained on the LM after a 24-h chilling period at 4 degrees C. The TS goats had higher plasma cortisol (P < 0.01) and glucose (P < 0.05) concentrations than NS goats. The rates of increase in plasma cortisol, glucose, and nonesterified fatty acid concentrations were greater in TS than in NS goats (stressor treatment x blood sampling time, P < 0.01). Muscle glycogen concentrations were greater (P < 0.05) in NS than in TS goats and higher (P < 0.01) in old vs. young goats; however, pH measured at 15 min and 24 h postmortem was not (P > 0.05) influenced by stressor treatment. Water-holding capacity of meat was not (P > 0.05) influenced by stressor treatment. Older goats had lower (P < 0.01) L* values and greater (P < 0.01) a* and chroma values than the younger goats. The a* and chroma values of loin cuts from young goat carcasses were lower in the TS than NS treatment groups, but this effect was absent in the old goat carcasses (stressor treatment x age, P < 0.05). Cooking loss percentages and shear force values for loin chops aged for 7 d were not (P < 0.05) affected by stressor treatment; however, old goats produced tougher (P < 0.01) loin chops than young goats. These results indicate that short-term preslaughter transport can cause noticeable changes in stress responses and muscle metabolism in goats.  相似文献   

19.
Information on the effect of road transportation conditions and lairage times on the meat quality of pork under South African conditions is very sparse. In this investigation, the effects of 2 road conditions (rough road with frequent stops - A; smooth road, few stops - B) and 2 lairage holding periods (2 h and 24 h) on the physical meat quality attributes of commercially produced pigs during summer (ambient temperatures > 30 degrees C) in the Western Cape (South Africa) were investigated. Pig meat from pigs transported on a road that caused more stress (A), had lower pH 45 (measured 45 min post mortem) values after 2 h lairage than pigs transported over a smoother road. Pigs B had a lower muscle pH 24 (measured 24 h post mortem) than group A, indicating that they had more glycogen reserves available for post mortem glycolysis. Road conditions A were more stressful resulting in a higher incidence of PSE pork, as shown by the percentage drip loss and the L* values. When the lairage period was increased to 24 h prior to slaughter, pigs transported under road conditions A had time to replenish their energy reserves and the pH 45, drip loss and L* values were within an acceptable range. However, pigs transported under road conditions B had lower pH 45 and higher pH 24 values, indicating that the lairage period was too long and that energy reserves were depleted in order to adapt to the stressful conditions. Results from this investigation indicate that improvement of the transport/road conditions will result in better pork quality.  相似文献   

20.
Cortisol concentrations in the saliva of two groups (N = 5/group) of prepubertal pigs were measured by radioimmunoassay. Samples were collected in the home pen under normal husbandry conditions and after a 24 h period when food and water were withheld. The pigs were then transferred to a transport simulator, which was left stationary (control) or set in motion (experimental), and further samples were taken 1 h later before the animals returned to their pens. In the following week, the two groups of pigs were mixed and saliva was collected over a 3 h period. Samples were also taken 2 days later after the pigs had been injected with a maximally stimulating dose of adrenocorticotrophin (ACTH). Transport simulation in deprived animals, and mixing, produced salivary cortisol levels similar to those seen after ACTH. Food and water deprivation alone also increased cortisol secretion whereas transport simulation in non-deprived animals had no effect. These results indicate that salivary cortisol estimation offers a non-invasive means of measuring stress responses in unrestrained pigs.  相似文献   

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