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《中国南方果树》2016,(5)
以湖南邵阳柑桔园筛选得到的尖孢镰刀菌Fusariumoxysporum为果胶酶产酶菌,开展单因素和响应面优化试验,以发酵液中果胶酯酶(PE)、果胶裂解酶(PL)、聚半乳糖醛酸酶(PG)活力为评价指标,探讨碳源量、氮源种类、氮源组成和氮源量对3种果胶酶活性的影响,并对优化培养基所产酶用于去除脐橙白皮层的效果进行了评价。结果表明:最佳培养基以硝酸铵/硫酸铵=3.02为氮源,氮源添加量为8.52g/L,碳源添加量为9.26g/L;其发酵液中的PG酶活为503.0U/mL,PL酶活为456.6U/mL,PE酶活为40.5U/mL,去除白皮层效果与商品酶法一致,具备工业化酶法去除脐橙白皮层的潜力。 相似文献
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高效获得金针菇闽金1号产漆酶条件,为实现漆酶大规模生产提供参考。采用单因素试验、P-B试验、响应面法（RSM）、中心组合试验（CCD）对闽金1号产漆酶的液体培养基组成进行优化。结果表明：最优碳源和氮源为蔗糖、蛋白胨+酵母粉,蔗糖、MgSO4、KH2PO4对漆酶活性有显著影响,最优产漆酶培养基组成为蔗糖22.27 g/L、MgSO40.96 g/L、KH2PO41.25 g/L,在此条件下,漆酶活性为945.981 U/L,是原始条件下（334.73U/L）的2.83倍。 相似文献
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蒙古口蘑液体发酵条件的优化 总被引：2，自引：0，他引：2
通过单因子试验和正交试验对蒙古口蘑(Tricholoma mongolicum)菌株Q97的发酵培养基组成及发酵条件进行了研究.结果表明:摇瓶发酵最佳培养基组成为豆饼粉 30 g/L,蔗糖 50 g/L,K2HPO4 1 g/L,CaCO3 1 g/L,pH自然,最佳发酵周期为13 d.在上述条件下发酵,最大生物量达到30 g/L,胞外多糖量达到100 mg/L以上. 相似文献
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为提高内生短短芽孢杆菌（Brevibacillus brevis）W4 菌株发酵液对番茄灰霉病菌（Botrytis cinerea）的抑菌活性，采用单因素试验与正交试验设计相结合，优化发酵培养基及发酵条件。结果表明，最佳发酵培养基为蛋白胨 2.0%、果糖 1.0%、NaCl 0.25%；最佳发酵条件为初始pH 8.0、接种种龄20 h、接种量5%、装液量50 mL（250 mL 三角瓶）、发酵温度35 ℃、摇床转速150 r·min-1、发酵时间24 h。产生的发酵液稀释25 倍对番茄灰霉病菌的抑制率达92.8%，比未经优化得到的发酵液提高26.9 个百分点。通过培养基和发酵条件优化，显著提高了短短芽孢杆菌W4 发酵液活性，为进一步提取分离抗番茄灰霉病菌的活性成分奠定了基础。 相似文献
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利用蛋白纤维平板法对1株蛹虫草(Cordyceps militaris)菌株液体发酵产生纤溶酶的碳氮条件进行系统优化;通过单因素试验筛选最佳碳源和氮源,再根据单因素试验结果进行正交设计实验优化该菌株产纤溶酶的最佳配方。结果表明:产纤溶酶的最佳碳源为乳糖,纤溶酶活性达118.69U/mL,其次是不加碳源的空白对照,活性为109.12U/mL;蚕蛹粉是该菌株产纤溶酶的最佳氮源,活性可达118.69U/mL,其次是酵母浸膏,活性为29.10U/mL;纤溶酶产量与菌丝体生物量之间无必然相关性;产纤溶酶的最佳培养基为乳糖10g/L、蚕蛹粉10g/L、酵母浸膏5g/L,获得的纤溶酶活性高达142.26U/mL。 相似文献
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以1株鸡腿菇菌株Cc-900为试材,采用平板培养和液体发酵培养的方法,研究不同浓度的外源硒对鸡腿菇菌株Cc-900的生长、富硒效果和菌丝合成蛋白质和多糖的影响,以期为富硒鸡腿菇的栽培和产品的开发提供参考依据。结果表明：鸡腿菇菌丝可耐受10 mg·kg-1硒浓度的固体培养基和最大硒浓度为3 mg·L-1的液体培养基,高浓度硒对菌丝活性损伤较大;当液体培养基中硒浓度为3 mg·L-1时,菌丝干质量可达1.97 g·L-1,菌丝干粉的硒含量为38.24 mg·kg-1,其硒富集系数可达12.75,菌丝中粗蛋白和多糖含量分别为2.58%和3 081.82μg·g-1,发酵液中可溶性蛋白质和多糖含量分别为50.98μg·mL-1和266.38μg·mL-1。液体发酵培养中硒浓度在3 mg·L-1以下可促进鸡腿菇菌丝的生长和硒的富集,同时促进菌丝中蛋白质的合成和释放,但会抑制菌丝中多糖的合成。 相似文献
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以灵芝和覆盆子为试材,通过L18 (37)正交实验设计对灵芝发酵生产覆盆子黄酮基础培养基进行了优化研究.结果表明:灵芝发酵生产覆盆子黄酮的基础培养基组合为葡萄糖20 g/L,黄豆粉15 g/L,玉米粉50 g/L,酵母膏1.0 g/L,KH2PO4 0.9 g/L,MgSO4·7H2O 0.2g/L,VB10.01 g/L,此培养基条件下,灵芝发酵覆盆子后黄酮含量达到4.58 mg/g,与对照组(不接种灵芝)相比含量提高96.57％. 相似文献
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园林草坪主要是指应用低矮、质优、扩展性强、质地纤细的植物作覆盖,并以它们大量的根、匍匐茎布满土表的地被.草坪是现代园林的一个重要组成部分,具有观赏、实用、改善生态环境等综合功能. 相似文献
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Rasa?Meskauskiene Oliver?Laule Nikolai?V?Ivanov Florian?Martin Markus?Wyss Wilhelm?Gruissem Philip?Zimmermann
Background
It is generally accepted that controlled vocabularies are necessary to systematically integrate data from various sources. During the last decade, several plant ontologies have been developed, some of which are community specific or were developed for a particular purpose. In most cases, the practical application of these ontologies has been limited to systematically storing experimental data. Due to technical constraints, complex data structures and term redundancies, it has been difficult to apply them directly into analysis tools.Results
Here, we describe a simplified and cross-species compatible set of controlled vocabularies for plant anatomy, focussing mainly on monocotypledonous and dicotyledonous crop and model plants. Their content was designed primarily for their direct use in graphical visualization tools. Specifically, we created annotation vocabularies that can be understood by non-specialists, are minimally redundant, simply structured, have low tree depth, and we tested them practically in the frame of Genevestigator.Conclusions
The application of the proposed ontologies enabled the aggregation of data from hundreds of experiments to visualize gene expression across tissue types. It also facilitated the comparison of expression across species. The described controlled vocabularies are maintained by a dedicated curation team and are available upon request.13.
以文冠果种子为试材,设置10种不同催芽方法,包括水浴加热、不同浓度的H2_SO_4、KNO_3、PEG-6000等试剂处理文冠果种子,诱导发芽后调查种子的发芽率、活力、发芽势等发芽参数,并对不同催芽方法进行了比较分析,筛选适合文冠果种子快速解除休眠的方法。结果表明:80℃水浴加热、4%KNO_3溶液、5%PEG-6000溶液、GA_3溶液、2%KNO_3溶液、10%PEG-6000溶液、4℃蒸馏水浸种、50%H_2SO_4溶液处理,均可不同程度的提高文冠果种子的发芽率,而高浓度H_2SO_4溶液处理对文冠果种子发芽有抑制作用;文冠果种子经80℃水浴加热10min处理可使发芽率提高到55.83%,为未处理的9.3倍。 相似文献
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Light emissions in lux from single incandescent lamps ranging from 60 to 200 watts were arranged in tables according to the lamp height above the ground. The method of calculating the desired lamp spacing to achieve a desired light intensity is illustrated. A table showing the reduced light emission due to various voltage drops is given along with precautions to avoid them. 相似文献
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《Scientia Horticulturae》2005,103(2):249-255
Bromeliad growers report severe leaf quality problems for Aechmea cultivars grown under commercial greenhouse conditions. In this research, a leaf damage ‘sensitive’ and ‘insensitive’ A. cultivar were compared for their C-metabolism and hydrophysiology under these greenhouse conditions. Stomata opening index indicated CAM (Crassulacean acid metabolism) for both cultivars, with 45–50% open stomata around 4:00 a.m. and 5–10% in the afternoon. Malic and citric acid were the major organic acids present in the leaves, with diurnal malic acid accumulation during the night. The leaf damage ‘sensitive’ cultivar showed higher malic acid accumulation than the ‘insensitive’ cultivar (200 μmol/gfw versus 170 μmol/gfw). Leaf osmotic potential and turgor pressure were linked with diurnal malic acid fluctuations: organic acid accumulation during the night generated high leaf turgor pressures (up to +0.86 MPa).A leaf damage sensitivity test was designed and confirmed previous experiences of several bromeliad growers. Both cultivars showed higher leaf damage percentages (98 and 78%) when leaf malic acid accumulation was high. We attribute this elevated sensitivity to lethal turgor pressures and consider them to be caused by the water capturing mechanism generated by high organic acid accumulation. Under current greenhouse conditions, this water capturing mechanism can be disastrous for plant leaf quality. Especially, under high relative humidity, hindering plant transpiration and stimulating water uptake by dew formation, leaf quality could be endangered. 相似文献
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