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1.
A sixty‐day feeding trial was conducted to determine the ascorbic acid (AA) requirement for growth of striped catfish, Pangasianodon hypophthalmus juveniles. Seven iso‐nitrogenous and iso‐energetic (370 g protein per kg and 19.6 MJ/kg) purified diets were prepared with different levels of ascorbic acid such as control (0), T1 (17.5), T2 (35), T3 (70), T4 (175), T5 (350) and T6 (700) mg ascorbic acid (L‐ascorbyl‐2‐polyphosphate) equivalent per kg diet. Fish with a mean body weight of 3.2–3.4 g were stocked (fifteen fish per tank) in triplicates following a completely randomized design. Each group was fed to satiation twice a day for 60 days. Significant differences were observed in growth, survival, body composition and metabolic enzymes activities with different dietary ascorbic acid levels. Maximum weight gain, specific growth rate (SGR) and protein efficiency ratio (PER) were found in fishes fed with 35 mg AA per kg diet, supported by best feed conversion. Fish fed a diet containing vitamin C had the highest activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) compared to those fed with vitamin C‐depleted diets. In this study, based on using broken‐line regression analysis, the dietary vitamin C requirement for growth of P. hypophthalmus juveniles was estimated to be in the range of 46–76 mg AA per kg, depending on the criterion used, growth and liver storage. Our results will be helpful for the formulation of cost‐effective ascorbic acid incorporated diets for striped catfish, P. hypophthalmus.  相似文献   

2.
ABSTRACT

Catla, Catla catla, and rohu, Labeo rohita, fry were cultured at 6,667,8,333, and 10,000/m3 in 15-L aquaria in recirculating systems for 30 days. Larvae were fed with exogenous live plankton. Cultures at 6,667 and 8,333 larvae/m3 showed significantly (P <0.05) higher survival and growth than larvae stocked at 10,000 larvae/m3 for both species. Food was more efficiently used in low stocking density, as evident from the significantly (P <0.05) lower values of feed conversion ratio in lower density compared to those for high stocking density. Specific growth rate of both species was high in the early stage and gradually declined along with the ontogenic development. Dissolved oxygen level was higher in the low density system than in the high density one. Values of phosphate and COD increased during the experiment. Ammonia, nitrite, phosphate, and COD levels were significantly (P <0.05) higher in the 10,000 larvae/m3 density system than in the other two systems for both species. Considering the survival and growth of fish and values of water quality parameters, it appears that stocking density can be raised up to 8,333 larvae/m3 with a recirculating system for both catla and rohu.  相似文献   

3.
In hatcheries, meagre Argyrosomus regius larvae still depend on an adequate supply of rotifers and Artemia, as no artificial diet can totally fulfil their nutritional requirements. However, production of live feed is highly expensive and demands intensive labour and specific facilities. This study investigated the effect of a dietary regime without the use of rotifers, to simplify the meagre larval rearing protocol. Two feeding treatments (T1 & T2) are compared to investigate their effects on survival and growth of meagre larvae. In T1, larvae were fed rotifers from 2 to 5 days post hatch (dph), and Artemia from 4 to 15 dph. In T2, larvae were kept under dark conditions and fed Artemia from 6 to 15 dph. Standard larval length (SL) was significantly higher in T1 (p < .01) until 8 dph in comparison with larvae reared initially without rotifers. No significant difference in SL was found among treatments (= .187) at 15 dph. Significant difference was found among treatments in survival rate at 15 dph (p < .003). The survival rate observed at 15 dph in T2 (30 ± 4.2%) represents an important finding, although the highest survival rate was observed in T1 (45.0 ± 3.4%). This study showed that it is possible to conduct larval rearing of meagre without using rotifers. Nevertheless, further research efforts are still needed to improve these results in comparison with the common larval rearing protocol.  相似文献   

4.
Kutum Rutilus frisii kutum is known as a valuable commercial species in the southern part of Caspian Sea. Artificial rearing of fry has been introduced as an alternative to supply kutum fry in order to restock the kutum population in the Caspian Sea. The aim of this study was to find the suitable time to transfer kutum larvae from live food to artificial feed. The experiment began on day 3 post- hatching and lasted for 21 days. Mean initial weight of larvae was 4.5 mg. Five experimental groups including Group A (zooplankton alone for 21 days), Group B (12 days zooplankton + 9 days artificial feed), Group C (8 days zooplankton + 13 days artificial feed), Group D (4 days zooplankton + 17 days artificial feed) and Group E (artificial feed alone for 21 days) were considered for this experiment. According to the obtained results, the specific growth rate of kutum larvae varied from 8.01 to 13.58% day−1, and the highest and lowest specific growth rate were found in A and E treatments, respectively. The lowest mean body weight (24.6 mg) was found in larvae fed on artificial feed for 21 days. However, survival rates of kutum larvae fed mixed zooplankton for 8 and 12 days (85.83 and 89.33%, respectively) were comparable with those of larvae fed live food during the entire experiment (91.6%). The lowest survival rate (69.16%) was found in larvae fed artificial feed during the entire experiment.  相似文献   

5.
Four feeding experiments, replacing 25% (T1), 50% (T2), 75% (T3) and 100% (T4), by dry weight, of the live feed Artemia nauplii for Cyclop‐eeze, a new larval feed that was claimed to contain the highest known levels of astaxanthin and omega‐3 polyunsaturated fatty acids, were compared against a control that was fed with Artemia and egg custard alone, to the larvae of giant freshwater prawn Macrobrachium rosenbergii (De Man 1879). Analysis of different production characteristics of the larvae revealed that the highest survival up to postlarvae (PL) stage was obtained for T2 in which 50% of the Artemia nauplii were replaced by Cyclop‐eeze [freeze‐dried (FD) deep frozen (DF)], and the highest astaxanthin content of the larval tissue obtained in T4 in which the larvae were fed 100% Cyclop‐eeze, although the survival rate was the lowest in this treatment. The costs of different treatments were also compared. The Artemia consumption million−1 larvae was the highest in control (11490 g), followed by T1 (8240 g), T2 (4990 g), T3 (3730 g) and T4, which completely replaced Artemia from stage 5 onwards (1830 g). The highest consumption of Cyclop‐eeze million−1 larvae was in T4 (1670 and 10 880 g), followed by T3 (850 and 5560 g), T2 (410 and 2690 g) and T1 (230 and 1490 g) of FD and DF, respectively. The astaxanthin contents of the late‐stage larvae fed under the four treatments were 24.90, 27.40, 28.60 and 35.60 μg g−1 tissue for T1, T2, T3 and T4, respectively, while that of the control was 23.70 μg g−1. The lowest cost of live feeds million−1 PL was obtained for T2 (US$ 428.60), followed by T1 (US$ 490.46), control (US$ 529.07) and T3 (US$ 583.26), while it was the highest for T4 (US$ 890.93). The results indicated that Cyclop‐eeze could economically replace Artemia nauplii at 50% level that could significantly improve the survival and carotenoid composition of the larvae of M. rosenbergii.  相似文献   

6.
To compare the growth performance of koi carp, Cyprinus carpio var. koi, produced in concrete tanks (2.13 × 0.91 × 1.22 m; capacity: 2,000 l each) and earthen ponds (9.1 × 6.10 × 1.07 m; capacity: 59650 l each), fish larvae (stocking size: 0.12 ± 0.008 g) were cultured for 11 weeks and individual weight gain, survival rate and number of marketable fish produced were compared among four management regimes for each culture system: (1) live zooplankton fed to fish larvae in ponds (PLF) and tanks (TLF); (2) application of poultry manure in ponds (PPM) and tanks (TPM); (3) application of cow manure in ponds (PCD) and tanks (TCD); and (4) a control treatment for ponds (PC) and tanks (TC), where a commercial feed was applied. There were three replicates for each treatment. Weight gain of koi carp was highest in the PLF treatment, followed in decreasing order by TLF, PPM, PCD, TPM, TCD, PC and TC treatments (P < 0.05). There was a significant difference in the survival of koi carp among the treatments, ranging from 67.83% in TC to 95.50% in PLF. The number of marketable fish produced was highest in the PLF treatment, followed in decreasing order by TLF, PPM and PCD treatments. However, none of the fish produced in the TPM, TCD, PC and TC treatments attained marketable size. Significantly higher (P < 0.05) values of pH and dissolved oxygen (for water samples collected weekly at 9 A.M.) were obtained in the live food and control treatments (for both tanks and ponds), compared to the manured treatments. The concentration of total alkalinity, BOD, PO4-P, NO3-N and specific conductivity were significantly higher (P < 0.05) in PPM and PCD, compared to other treatments. NO2-N and NH4-N values were significantly higher (P < 0.05) in TPM and TCD, than other treatments. The results suggest that introduction of live zooplankton into culture units result in higher growth of koi carp larvae compared to manure based systems. Earthen ponds appeared to be better alternative to concrete tanks for manure application through maintenance of better water quality due to their higher assimilatory capacity and greater abundance of plankton which resulted in better growth of cultured fish.  相似文献   

7.
Adequate nutrition is a principal factor in controlling survival and growth in crustacean larval culture. The present study examined the effects of starvation before or after feeding on survival, total intermoult period and postmoult size of phyllosoma of the western rock lobster, Panulirus cygnus. Individually held instar 1 larvae were reared at 25 °C and submitted to initial periods of starvation to determine the 50% level of the point-of-no-return (PNR50), and initial periods of feeding to determine the 50% level of the point-of-reserve-saturation (PRS50). As the initial starvation periods before feeding increased, the total intermoult period for instar 1 larvae that survived increased. The PNR50 was 4.6 days. The different initial feeding periods before starvation resulted in a difference in postmoult size of larvae after moulting to instar 2, but had little effect on the total intermoult period. Larvae fed for the shortest periods were significantly smaller than those fed longer. The PRS50 was 3.6 days. For both treatments, limited effects of the starvation or feeding regimes were apparent for larvae that moulted to instar 2 and continued development to instar 3. When larvae were fed before PNR50, there were no delayed effects of the initial starvation period. Initial feeding periods longer than 5–6 days did not significantly affect larval survival or growth. This information will be useful in the design of feeding regimes in phyllosoma culture.  相似文献   

8.
Sugarcane bagasse (T1), paddy straw (T2) and their mixture (T3) were added to mud‐bottomed, manured cement tanks in triplicate to study the effect on bacterial biofilm production and growth and survival of rohu, Labeo rohita. All tanks were stocked with fingerling rohu at 10 000 ha?1. The total plate count (TPC) of bacteria in water and on substrate did not differ significantly between treatments. The mean phytoplankton and zooplankton density in water was the highest in T2, followed by T3 and T1. The growth of fish was significantly (P<0.05) higher in T2 and T3 compared with T1. Significantly higher RNA values were recorded in T2 and T3 compared with T1. Similarly, T2 and T3 registered higher DNA values than T1. RNA:DNA ratio was higher in T2 compared with T1. However, T3 did not differ significantly from T1 and T2. Both intestinal and hepatopancreatic protease and lipase activity was higher in T1 and T2 compared with T3 and both differ significantly from T1. Intestinal amylase activity was higher in T2 and T3 compared with T1, whereas hepatopancreatic amylase activity was higher in T2 compared with T1 and T3. The results demonstrated that production of L. rohita can be significantly increased by the introduction of biodegradable substrates into culture systems where fertilization is employed.  相似文献   

9.
The relative contributions of live Artemia metanauplii and an inert diet for growth of Senegalese sole larvae and postlarvae were assessed through the analysis of carbon stable isotopes ratios (δ13C) in both diets and whole larval tissue. Larvae were reared on four dietary regimes: 100% live prey (rotifers and Artemia), 100% inert formulated diet and two co-feeding regimes of 70:30 and 30:70 ratios of Artemia and inert diet, respectively. Larvae from the live food regime and both co-feeding regimes showed a steep increase in δ13C from 10 days after hatching (DAH) as a result of the onset and continuation of Artemia consumption. From 12 DAH fish larvae from all the regimes showed significant isotopic differences as their δ13C increased to final asymptotic values of − 15.1, − 15.6 and − 16.3‰ in the live food, 70:30 and 30:70 regimes, respectively. Carbon turnover rates in larvae from both live food and co-feeding regimes were relatively high (0.071 to 0.116 d− 1) but more than 90% of the observed change in fish tissue isotopic values was accounted for by the retention of carbon in new tissue growth. A two-source, one-isotope mixing model was applied to estimate the nutritional contribution of Artemia and inert diet to postlarvae growth in the co-feeding regimes. At 23 DAH, the relative contribution of live and inert diets to tissue growth in larvae was respectively, 88 and 12% for the 70:30 co-feeding regime and 73 and 27% for the 30:70 co-feeding regime. At 17 DAH, the estimated proportion of tissue carbon derived from the inert diet was higher at 23 and 38% for the 70:30 and 30:70 regimes, respectively. The results suggest that co-feeding regimes in Solea senegalensis larvae may be adjusted to meet ontogenetic changes in the capacity for larvae to utilise inert diets. The contrasting levels of carbon isotope discrimination between diet and tissue in larvae reared on either 100% live feed or 100% inert diet indicate relatively poor utilization of nutrients from the inert diet. The use of isotopic discrimination factors as potential indicators of the digestive physiological performance of a consuming organism in regards to its diet is discussed.  相似文献   

10.
Problems of limited number of dry feeds as supplement or replacement of live feeds have led to poor larval nutrition in many species of fish. Therefore, the suitability of co‐feeding 8‐day‐old African catfish (Clarias gariepinus) posthatch larvae using live feed (Artemia salina) and formulated dry diet containing freshwater atyid shrimp (Caridina nilotica) during weaning was investigated. The experiment ended after 21 days of culture and respective groups compared on the basis of growth performance, survival, feed utilization and nutrient utilization. Larvae co‐fed using 50%Artemia and 50% formulated dry diet resulted in significantly (P < 0.05) better growth performance, food gain ratio (FGR), protein efficiency ratio (PER) and productive protein values (PPV) than other treatments. The lowest growth performance occurred in larvae weaned using 100% formulated and commercial dry diets. Better survival of over 90% was obtained in larvae weaned using 50%Artemia and 50% dry diet, while abrupt weaning using 100% dry diets resulted in lower survival (<75%). These results support a recommendation of co‐feeding C. gariepinus larvae using a formulated dry diet containing C. nilotica and 50% live feed when weaning is performed after 8 days posthatching period.  相似文献   

11.
We evaluated the growth and survival rate of sterlet (Acipenser ruthenus) larvae fed Artemia nauplii enriched with Olioω3 or Red Pepper commercial emulsions (BernAqua NV, Belgium). Sterlet larvae, 0.022 ± 0.002 g body weight, were randomly assigned to one of three feeding regimes with two different feeding durations. After administering live feed for 7 or 14 days, larvae were weaned onto commercial food and reared to 36 days posthatching (28 days of feeding). There were no significant differences in body weight among groups at the end of the trial. A significantly higher survival rate (p < 0.05) was observed in larvae fed Artemia enriched with Red Pepper for 14 days compared to other feeding regimes. Based on the analysis of growth parameters, we can conclude that 7 days of live feeding to be sufficient for efficient rearing of sterlet larvae. And longer duration of live feeding with use of special enrichment can be recommended for a higher survival rate.  相似文献   

12.
Experiments were conducted with larvae of theblack-lip pearl oyster, Pinctadamargaritifera fed on a regime of living algae(an equal mixture of Tahitian Isochrysisaff. galbana and Pavlova salina)either partially or completely substituted withheterotrophically grown spray-dried algae (Tetraselmis suecica). Two experiments wereconducted; in the first, 1-day old larvae werecultured for 13 days and, in the second, 13days old larvae were culture for 7 days. Inboth experiments larvae were fed the followingproportions of live algae (LA) and dried Tetraselmis (DT); (1) 100% LA, (2) 75% LA +25% DT, (3) 50% LA + 50% DT, (4) 25% LA +75% DT; and (5) 100% DT. The optimal dietsfor maximum larval growth were 100% LA and75% LA + 25% DT resulting in larvae with amean shell length (SL) of 132 ± 3.8 and 131± 2.7 µm, respectively, at day 13. Thisshowed that 25% substitution of livemicro-algae with DT is possible withoutaffecting growth of P. margaritiferalarvae of less than 150 µm SL. There was nosignificant difference in survival of P.margaritifera larvae fed 100% LA, 75% LA +25% DT and 50% LA + 50% DT over a period of13 days. Highest survival (43.7%) occurred inlarvae fed 100% DT while lowest (approximately18%) occurred in controls (50% livemicro-algae and unfed) during the firstexperiment. The result of the second experimentindicated that with increasing size, larvae arecapable of accepting a higher proportion ofdried Tetraselmis in their diet. Therewere no significant differences in larvalgrowth or survival, between treatments where LAwas partially and completely substituted dietswith DT.  相似文献   

13.
An experiment was conducted from December 2003 to April 2004 to observe the over‐wintering growth of freshwater prawn, Macrobrachium rosenbergii, with catla, Catla catla and rohu, Labeo rohita in polyculture using formulated diets. The study was conducted in eight experimental ponds, each 80 m2. Three experimental diets containing 30% protein were prepared using fish meal, meat and bone meal, mustard oilcake, rice bran, wheat bran and molasses (binder), and assigned to treatments T1, T2 and T3 respectively. A commercial diet from Saudi‐Bangla Fish Feed was assigned to T4 (reference diet). Each treatment had two replicate ponds. Juvenile prawns and catla and rohu fingerlings (initial weight 1.60±0.10, 30.0±1.2 and 25.0±1.1 g respectively) were stocked at a ratio of 2:1:1 (prawn:catla:rohu). A total of 160 prawn and fish (20 000 ha?1) were stocked in each pond. Fish were fed twice daily at 3% body weight (b.w.) for the first 3 months and 5% b.w. for the last 2 months. Prawns in T1 fed diet 1 had significantly higher (P<0.05) weight gain compared with that of T3. The reference group and T2 had intermediate values not significantly different from either. Weight gains of catla and rohu were significantly higher in T1. The feed conversion ratio values of different diets ranged between 1.89 and 2.13. Survival (%) ranged from 90.0% to 95.0% for catla, 87.5% to 92.5% for rohu and 70.0% to 76.3% for M. rosenbergii, and there were no significant differences (P>0.05) among different treatments. Total production ranged between 2196 and 2679 kg ha?1, with T1 showing significantly higher production and net profit (taka 56 531.9 ha?1). The results of the study demonstrated that it is possible to culture M. rosenbergii with carp in polyculture during the winter utilizing the late‐produced PLs. Further study is needed to determine the optimum stocking density of M. rosenbergii in carp polyculture.  相似文献   

14.
This study was carried out to test the effect of triiodothyronine (T3) on the growth and survival of larval striped bass (Morone saxatilis). Growth and survival of striped bass held in 5 ppt seawater and treated with various doses of T3 were measured beginning at 5 and 16 days after hatching. Body content of T3 was measured by radioimmunoassay. T3 dissolved in the 5 ppt seawater was taken up by larval striped bass in a dose-dependent manner, and affected the growth and survival of the fish. At 5 days after hatching, T3 at 100 ng ml–1 and 50 ng ml–1 retarded the growth of larval striped bass and caused a lower survival rate than T3 at 25 ng ml–1 or the control treatment. At 16 days after hatching, T3 at 100 ng ml–1 retarded the growth of larval fish and caused a higher mortality. T3 at 10 ng ml–1 and 1 ng ml–1 did not show any effect on either survival or growth. Body content of T3 returns to control levels within days following end of treatment. The results indicate that exogenous T3 can be detrimental to the growth and survival of larval striped bass.  相似文献   

15.
This paper reports the triiodothyronine’s (T3) effects on the early growth and survival of piracanjuba (Brycon orbignyanus) produced from fertilized eggs hormone exposed. The study was carried out in two phases. In the first phase, eggs divided in 6 batches were immersed in T3 solutions: 0.01; 0.05; 0.1; 0.5 ppm; 1 ppm and control (no T3). After a 15-min immersion, eggs were transferred to incubators where larvae were kept up to 72 h after hatching. Larval weight, length and yolk sac volume were determined every 12 h. Sixty and 72 h after hatching, larvae exposed to 0.5 ppm T3 were significantly heavier than the others, and those exposed to 1 ppm T3 showed the lowest weight. The yolk sac absorption was not affected. In the second experimental phase, the resulting fry from the first phase were stocked into 3 boxes per treatment (5 larvae L−1) and fed with plankton, fish larvae and feed prepared in the hatchery (48% CP) in the first 3 days, plankton and feed from the 4th to the 10th day and only feed in the next (last) 5 days. Fry weight, length and specific growth rate were determined at 1, 5, 10 and 15 days. Survival was calculated in the last day. In the 15th day, fry length did not differ among treatments but the weight of the control group was higher. Higher survival in the T3-treated groups suggested lower predation among fry. The results allowed us to conclude that there was no expressive effect of T3 on the growth, but it improved the survival of the piracanjuba progeny.  相似文献   

16.
The effects of four different ionic composition low salinity water (T1, T2, T3, and T4), on growth and survival of Litopenaeus vannamei marine shrimp juveniles were investigated. Shrimp culture in seawater (Tm) was used as control treatment. The results indicated that there were no significant difference (P < 0.05) in growth, survival, production, and feed conversion ratio (FCR) of L. vannamei juveniles reared in the different treatments, but significant differences (P < 0.05) were observed between each of them when compared with seawater (Tm). After 84 days, culture shrimp grew from 0.02 to 7.58 g in T1. The lowest growth rate was attained in T3 (0.57 g/week), in which potassium and calcium ions concentrations were the lowest (0.58 and 28.00 mg/L, respectively). The recorded survival rate (76.35% to 79.55%) is considered well for the 84 days growout period, although it was 7.6% lower than that recorded in the control treatment. Although there were no significant differences (P < 0.05) in growth with respect to the ionic composition of the four treatments, there was a trend of increasing growth in relation with the ionic ratio found in the seawater (Tm). This aspect should be evaluated more closely in future research.  相似文献   

17.
A study was conducted to optimize stocking density of freshwater prawn, Macrobrachium rosenbergii, in carp polyculture for 3 months in 10 experimental ponds of 80 m2. Five stocking densities of prawn, 2500, 5000, 7500, 10 000 and 12 500 ha?1, were assigned to treatments T1, T2, T3, T4 and T5 respectively. The densities of catla, Catla catla, rohu, Labeo rohita and silver carp, Hypophthalmicthys molitrix, were 2500, 5000, and 2500 ha?1, respectively, in each treatment. Each treatment had two replicate ponds. The mean initial weights of prawn, catla, rohu and silver carp were 1.1±0.02, 8.28±0.1, 25.2±1.1 and 36.32±1.2 g respectively. A pelleted diet containing 30% protein was prepared using fish meal, meat and bone meal, mustard oilcake, rice bran, wheat bran and molasses, and was fed twice daily at a rate of 5% of fish biomass. Water quality parameters were measured fortnightly and the ranges of temperature, pH and dissolved oxygen were 27.5–1.3°C, 6.9–8.6 and 4.5–8.6 mg L?1 respectively. Feed conversion ratios ranged from 2.05 to 2.20 among the treatments. Per cent survival (%) of prawns ranged from 72% to 78%, while it varied from 80% to 93%, 90% to 95% and 90% to 92% for catla, rohu and silver carp respectively. The results showed that there were no significant differences among the weight gains of prawn and carp in different treatments. However, the overall total production of prawn and fish together was significantly (P<0.05) higher in T3 and T4 compared with other treatments. The total production for 3 months ranged between 2618 and 2916 kg ha?1. The production of prawn was significantly higher (361.3 kg ha?1) in T5 with a highest stocking density of 12 500 prawn ha?1. Although there was no significant difference (P>0.05) between the total production of prawn and fish together in T3 and T4, the highest net profit (Tk. 69 006 ha?1) was obtained in T4. Therefore, from the result of the study it may be concluded that a stocking ratio of 4:1:2:1 of prawn:catla:rohu:silver carp at a total density of 20 000 ha?1 may be recommended for prawn–carp polyculture in ponds.  相似文献   

18.
In this study, the influence of commercial probiotic, Lactobacillus spp., supplementation was investigated on growth parameters and digestive enzyme activities in gilthead sea bream, Sparus aurata, during larval development. All experiments were triplicated and designed in three different administrations of probiotic from 3 days after hatching (DAH) concurrently with starting of exogenous feeding. In the first group, probiotic was added to live food (rotifer and Artemia). In the second group, probiotic was supplemented directly to both live food and water. In the third group, probiotic was added directly to water. Also, no probiotic treatment was maintained in control group. Total bacterial counts among probiotic probiotic-supplemented groups were significantly different from total bacterial counts in controls in water and digestive tract of larvae (p < 0.05). The mean of total bacterial counts in control was approximately 4 × 104-fold increased from the experimental groups in the sea water (p < 0.05). Besides, mean digestive enzyme activities of all probiotics treatment groups were significantly different (p < 0.05) with that of the control. Except probiotic water supplementation group, in all treatments, the specific activities of pancreatic and intestinal enzymes were significantly higher (p < 0.05) in larvae to which probiotic had been supplemented by live food and live food with water. Also, S. aurata larvae that had probiotic administered by live food with water demonstrated significant (p < 0.05) increases in both survival (13–105% higher) and specific growth rate (2–9% higher) as compared to controls. As a result, supplementation of probiotic to directly tank water could not significantly increase growth parameters and digestive enzyme activities and therefore, administration of probiotics by this method would not be effective in terms of husbandry parameters and nutritional condition.  相似文献   

19.
The barber goby Elacatinus figaro is an endangered fish, endemic to Brazil and very important to the aquarium trade. One of the bottlenecks for its production in captivity is the larviculture, which is characterized by high mortality rates, especially in the first week after hatching, and prior to metamorphosis. The experiment evaluated the effect of triiodothyronine hormone (T3) on survival, growth and metamorphosis of barber goby. Larvae of 14 days after hatching (DAH) were immersed in three concentrations of T3, in triplicate: TC (control) – 0; T01 – 0.01; T025 – 0.025 and T05 – 0.05 mg/L. An additional replicate of each treatment was performed for sampling for the histological analysis of the thyroid follicles in 24 DAH larvae. The survival rates in TC, T01, T025 and T05 were 24%, 54%, 36% and 37% respectively, without significant differences between the treatments (p > 0.05). Regarding larval growth, the highest length values were obtained in TC. In T025 and T05, larval metamorphosis was anticipated in up to 11 days in relation to TC and T01. In T01 larvae, follicles were numerous with the presence of reabsorption vesicles in the colloid periphery, indicating increased production of thyroid hormones (THs), associated with the larval metamorphosis process. In T05, a follicle reduction in number and size was observed, given evidence of the end of metamorphosis. The use of 0.025 and 0.05 mg/L of T3 anticipated metamorphosis in barber goby larvae, shortening the larviculture period for this species.  相似文献   

20.
Polyculture of olive barb with indigenous major carps was carried out for 8 mo at different densities in earthen ponds. All the experimental ponds were stocked with fingerlings of major carps viz., catla, Catla catla, and rohu, Labeo rohita, at the rate of 3750/ha each. In addition, olive barb, Puntius sarana, was stocked at the rate of 10,000, 12,500, and 15,000 fingerlings/ha in treatment‐1 (T1), treatment‐2 (T2), and treatment‐3 (T3), respectively. Fish in all the ponds were fed with supplementary feed comprising of rice bran (70%), mustard oil cake (25%), and fish meal (5%) at the rate of 3–6% of the estimated body weight. Physicochemical parameters and plankton populations of pond water were within the acceptable range for fish culture. The mean final weight, weight gain, and specific growth rate of catla were very similar in all the treatments while those for rohu and olive barb were significantly higher in T1 than in T2 and T3. The gross and net productions in T1 were significantly higher than in T2 and T3. The net benefit was also highest in T1 followed by T2 and lowest in T3. Under the conditions of this experiment, growth, production, and benefits were greatest at a stocking density of olive barb at 10,000/ha in polyculture with other major carps.  相似文献   

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