共查询到19条相似文献，搜索用时 312 毫秒
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液相色谱-荧光检测法检测水产品中甲砜霉素、氟甲砜霉素及代谢物氟甲砜霉素胺残留 总被引：2，自引：0，他引：2
建立了以带荧光检测器的液相色谱检测水产品中甲砜霉素、氟甲砜霉素与氟甲砜霉素胺残留的新方法.采用碱性乙酸乙酯-乙腈提取试样中残留的化合物,凝胶渗透色谱(GPC)净化,荧光检测器的激发与发射波长分别为225 nm与295 nm,可疑样品以液相色谱-质谱/质谱进行确证.在添加浓度为0.050～2.0 mg/kg时,平均回收率在86.4%～96.8%之间,相对标准偏差(RSD)在5.63%～9.71%之间(n=5);甲砜霉素、氟甲砜霉素在0.20～5.0 mg/L范围内、氟甲砜霉素胺在0.10～5.0 mg/L范围内有良好的线性关系,甲砜霉素与氟甲砜霉素的检测限达0.020mg/kg、氟甲砜霉素胺的检测限达0.010 mg/kg. 相似文献
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以试管二倍稀释法测定替米考星 (Tilmicosin)及对照药物强力霉素、红霉素、泰乐菌素对鸡败血霉形体的最小抑菌浓度分别为 0 .0 0 6 2 5、0 .0 2 5、0 .0 2 5、0 .1m g/ L ,进行了替米考星对鸡败血霉形体的体内药效学研究。结果 ,替米考星以 5 0、75、10 0 mg/ L 饮水给药 5 d,对实验性感染鸡败血霉形体有显著疗效 ,用药组增重均显著高于对照组 (P<0 .0 5或 P<0 .0 1) ,并能明显减少抗体阳性反应率 ,极显著地减少气囊损伤记分 (P<0 .0 1) 相似文献
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氟甲砜霉素对鸭大肠杆菌病的药效研究 总被引：1，自引：0，他引：1
为了探讨氟甲砜霉素对鸭大肠杆菌病的疗效进行了本研究。用试管2倍稀释法测定氟甲砜霉素及对照药氯霉素对大肠杆菌的最小抑菌浓度，然后用氟甲砜霉素，氯霉素进行混饲给药5d的疗效试验，试验结果表明，氟甲砜霉素，氯霉素对大肠杆菌O78株的最小抑菌浓度分别为6mg/L,8mg/L,100,200,400mg/kg氟甲砜霉素混饲给药对鸭大肠杆菌病的有效率分别为30．3％，93．9％，100％,400mg/kg氯霉素的有效率为93．9％，试验各组之间鸭的增重差异不显著。 相似文献
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用复方甲砜霉素注射液以高(0．8ml／kg．b．w)、中(0．4ml／kg.b．w)、低(0．2ml／kg.b．w)三个剂量对人工感染鸡巴氏杆菌病的鸡群肌内注射治疗，同时设立甲砜霉素对照组。试验结果表明：用药2d后，复方甲砜霉素注射液高、中剂量组的有效率分别为93．3％、93．3％，效果优于对照药物甲砜霉素(70．0％)， 相似文献
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《畜牧兽医学报》2015,(10)
为验证育雏早期应用黄芪多糖及甲砜霉素对ALV-J先天性感染雏鸡发病作用的影响,通过5日龄SPF鸡胚卵黄囊接种1 000TCID50ALV-J,鸡胚出壳后随机分为3组,两试验组分别定期口服黄芪多糖和甲砜霉素,一组作为病毒感染对照。对试验雏鸡泄殖腔排毒规律、病毒血症、疫苗免疫水平、免疫器官指数、体重、肿瘤发生率、肿瘤类型等进行动态测定,评估黄芪多糖和甲砜霉素对雏鸡先天感染ALV-J发病作用的影响。结果表明,两种药物对ALV-J病毒血症、ALV-J抗体及疫苗免疫抗体的产生无显著影响。黄芪多糖组与病毒感染对照组体重差异不显著;甲砜霉素组早期体重明显高于其他两组,但后期体重却明显低于另外两组。甲砜霉素组育雏早期血液中白细胞和淋巴细胞的水平显著低于另外两组,60日龄后脾和法氏囊的免疫器官指数显著降低,与另外两组差异显著。肿瘤发生率以甲砜霉素组最高,达28.57%(10/35),其次是病毒感染对照组,为20%(7/35),黄芪多糖组最低,仅为11.43%(4/35)。另外,甲砜霉素组肿瘤类型多,出现血管瘤、髓样细胞瘤、淋巴肉瘤、纤维肉瘤和腺癌,并且肿瘤进展快,病变重。育雏早期应用甲砜霉素会导致雏鸡免疫抑制,促进ALV-J的致瘤作用,患病鸡肿瘤呈多样化特征。黄芪多糖对ALV-J的致病性有一定的抑制作用,但未发现对病毒血症的影响。两种药物对ALV-J的致病作用的影响机制有待于进一步研究探讨。 相似文献
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氟甲砜霉素对实验性鸡巴氏杆菌病的药效研究 总被引：1，自引：0，他引：1
以两倍稀释法测定氟甲砜霉素及甲砜霉素对鸡巴氏杆菌的最小抑菌浓度分别为０．２５μｇ／ｍＬ和１．２５μｇ／ｍＬ。按１０,２０和３０ｍｇ／ｋｇ的氟甲砜霉素及３０ｍｇ／ｋｇ的甲砜霉素分别给实验性巴氏杆菌病患鸡肌注,每天给药一次,共５ｄ。药物对鸡巴氏杆菌病的治愈率分别为９３．３％,１００％,１００％和１００％,而感染对照组鸡全部死亡。用药组与感染对照组间疗效差异极显著 相似文献
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试验将鸡肉样品制成匀浆,用乙酸乙酯提取,正己烷去脂后用乙酸乙酯反萃取,经固相萃取柱(SPE)净化,甲醇洗脱,采用HPLC进行检测。甲砜霉素和氟甲砜霉素在同时检测时的保留时间分别为7.30min和17.60min,测得甲砜霉素与氟甲砜霉素的检测限达10ng/g。在添加浓度为0.005～0.02mg/kg时,平均回收率在65.84%～81.18%之间,相对标准偏差(RSD)在5.6%～13.2%之间(n=5);甲砜霉素、氟甲砜霉素在0.01～1.0mg/L范围内有良好的线性关系。 相似文献
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根据中兽医辨证论治理论、体外抑菌试验及中草药体内筛选试验结果 ,组成 2个复方制剂对人工感染肠炎沙门氏菌病鸭作治疗保护试验 ,同时测定感染病鸭口服中草药制剂后血清中超氧化物歧化酶 (SOD)活性和丙二醛 (MDA)的含量 ,对药物作用机理作初步探索。结果显示“肠炎康 1号”高、中、低 3个剂量组和“肠炎康 2号”高剂量组及氟甲砜霉素对照组对人工感染肠炎沙门氏菌病鸭均有较好的治疗作用 ,与感染对照组的自然保护率 (72 .7%)比较 ,差异极显著 (P<0 .0 1 ) ,而“肠炎康2号”中、低剂量组与感染对照组比较 ,差异显著 (P<0 .0 5 )。中草药制剂在促进病鸭康复的过程中影响了体内自由基的活动。 相似文献
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氟砜霉素的急性毒性和亚慢性毒性试验 总被引：5，自引：0，他引：5
氟砜霉素对大鼠的急性毒性试验结果表明 ,氟砜霉素对大鼠的经口 LD5 0 大于每千克体重 50 0 0 mg。亚慢性毒性试验的结果表明 ,按每千克体重 1 0 0 mg的剂量应用氟砜霉素 3 0 d以上 ,会减少大鼠的增重 ,降低骨髓的造血机能 ,损伤肝脏 ,严重损伤肾脏及睾丸 ,但对脾脏、肺脏、雌性生殖器官、大脑无损伤。氟砜霉素 50mg/ kg的剂量对肾脏有轻度的损伤 ,在 3 0 d时可减少大鼠的增重 ,但对其他组织器官以及骨髓造血机能均无影响。氟砜霉素 3 0 mg/ kg的剂量应用 60 d,对大鼠的增重、骨髓造血机能及各组织器官均无影响 相似文献
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不同剂量氟苯尼考对猪血液生化指标及猪瘟抗体的影响 总被引：1，自引：0，他引：1
40头70日龄健康猪随机分为高、中、低剂量组和对照组,每组10头,各试验组注射猪瘟弱毒疫苗(2头份/猪),同时高、中、低剂量组分别按每千克体质量于饲料中加入120、60、30 mg氟苯尼考,连续给药7 d,停药后1、8、15、22、29 d,对相关的血液生化指标以及猪瘟抗体水平进行检测分析.结果表明,停药后1、8 d,所有氟苯尼考添加组的猪瘟抗体滴度下降,仅120 mg/kg组差异显著(P<0.05),停药15 d后抗体水平恢复至正常水平;60和120mg/kg给药组TP含量在停药后1、8 d均显著下降(P<0.05);所有给药组的ALT活性在所有检测时间内均显著上升(P<0.05或P<0.01);所有给药组的γGT活性在停药后1 d均显著上升(P<0.05),而60和120 mg/kg组γGT在第8天仍显著上升(P<0.05);所有给药组的BUN含量在停药后1 d均显著升高(P<0.05或P<0.01);AST、LDH、CHE活性和ALB、CRE含量与对照组相比无显著差异(P>0.05);表明每千克体质量60 mg以上氟苯尼考在饲料中饲喂7 d后,对部分生化指标有一定的影响,应引起重视. 相似文献
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Dowers KL Olver C Radecki SV Lappin MR 《Journal of the American Veterinary Medical Association》2002,221(2):250-253
OBJECTIVE: To compare treatment with enrofloxacin and doxycycline with no treatment in cats experimentally infected with Haemobartonella felis. DESIGN: Prospective case-control study. ANIMALS: 16 cats. PROCEDURE: Cats were inoculated with large-form H. felis from a chronically infected donor. Cats were assigned to 1 of 4 treatment groups: doxycycline (5 mg/kg [2.3 mg/lb], p.o., q 12 h), low-dose enrofloxacin (5 mg/kg, p.o., q 24 h), high-dose enrofloxacin (10 mg/kg [4.5 mg/lb], p.o., q 24 h), and an untreated control group. Clinical signs, Hct, blood smears, and a polymerase chain reaction (PCR) assay were used to monitor progression of the infection. RESULTS: All cats were confirmed to be infected with H. felis via blood smear evaluations and PCR assay results. Treatment had no effect on Hct during the intratreatment period, but Hct values were significantly greater in the low-dose enrofloxacin group, compared with the control group, during the posttreatment period. During the intratreatment period, H. felis organism counts per 1,000 RBC in the doxycycline treatment and the high-dose enrofloxacin treatment groups decreased at a significantly faster rate than those in the control group. In the posttreatment period, organism counts in the doxycycline treatment group and the low- and high-dose enrofloxacin groups decreased at significantly faster rates than counts in the control group. There was no significant effect of treatment on the number of positive PCR assay results. Two cats treated with enrofloxacin and 1 cat treated with doxycycline completely cleared the H. fe is organism despite presumed immunosuppression caused by glucocorticoids. CONCLUSIONS AND CLINICAL RELEVANCE: Results support the hypothesis that enrofloxacin has anti-H. felis effects. 相似文献
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甜菜碱对生长猪的生长性能和胴体组成的影响 总被引：1，自引：0，他引：1
选用 96头杜长大生长猪 ,随机分成 4组 ,以研究日粮中添加不同水平甜菜碱 (10 0 0、15 0 0、2 0 0 0 mg/kg)对猪生长性能、胴体组成及肉质的作用效果。试验结果表明 ,与对照组相比 ,日粮中添加 10 0 0 mg/kg和 15 0 0 mg/kg甜菜碱组猪日增重分别提高 13.2 0 % (P<0 .0 1)、9.2 8% (P<0 .0 5 ) ,日均采食量分别增加 7.30 % (P<0 .0 5 )、7.33% (P<0 .0 1) ,料重比分别下降 7.93% (P<0 .0 1)、6 .5 5 % (P<0 .0 5 )。此外 ,10 0 0 mg/kg甜菜碱组猪的胴体瘦肉率和眼肌面积比对照组分别提高 7.15 % (P<0 .0 5 )、19.12 % (P<0 .0 5 ) ,胴体脂肪率与背膘厚分别减少 2 7.2 1% (P<0 .0 5 )、14 .86 % (P<0 .0 5 ) ;背最长肌中的肌红蛋白和肌内脂肪分别提高 34.2 1% (P<0 .0 1)、2 9.5 6 % (P<0 .0 1) 相似文献
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《中国畜牧杂志》2019,(9)
本试验旨在研究富锗酵母培养物对下笼蛋鸡肉品质及肠道的影响。选取505日龄健康的海兰褐壳蛋鸡480只,随机分为4组,每组5个重复,每个重复24只鸡。对照组饲喂基础日粮,试验组在基础日粮中分别添加12、16、20 mg/kg的富锗酵母菌培养物。预试期7 d,正试期为42 d。结果表明:与对照组相比,12、16、20 mg/kg组腿肉的蒸煮损失分别降低45.2%、33.6%、39.7%(P<0.01),pH均高于对照组(P<0.01);16mg/kg组腿肌的亮度(L~*)低于对照组(P<0.05);12、16、20 mg/kg组腿肌的剪切力分别比对照组低44.13%、56.07%、50.60%(P<0.01);各试验组胸肌pH均高于对照组(P<0.01),16mg/kg组的pH高于12、20mg/kg组(P<0.05);与对照组相比,16mg/kg组乳酸菌数量升高(P<0.05);空肠中,各试验组的绒毛长度高于对照组(P<0.01);16、20 mg/kg组绒毛长度/隐窝深度高于对照组(P<0.01)。结果显示,日粮中添加富锗酵母培养物可以改善下笼蛋鸡小肠的形态结构和肠道菌群平衡,提高鸡肉品质,建议添加量为16 mg/kg。 相似文献
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The effect of enrofloxacin therapy was investigated in 110 male double-muscled cattle weighing 275 +/- 3 kg, during a spontaneous outbreak of shipping fever occurring 11 +/- 2 days after they arrived in the feedlot. Forty-six diseased animals were divided randomly into three groups A, B and C, containing 17, 19 and 10 animals, respectively; the animals in group A were injected intramuscularly once daily for three consecutive days with 2.5 mg/kg of enrofloxacin, those in group B with 5 mg/kg of enrofloxacin and those in group C with 10 mg/kg of oxytetracycline. Clinical, serological, production and respiratory functional observations were recorded. The animals were clinically cured after the three day treatment except for three in group A and two in group C. These five animals made a clinical recovery after a three day booster treatment with a dose of 5 mg/kg enrofloxacin. The changes in respiratory gas exchange values induced by shipping fever were completely reversed 15 days later, suggesting that there had been no irreversible lung damage. The daily weight gains and the arterial blood gas values of the three groups of treated cattle were not significantly different. The high efficacy of the low dosage of enrofloxacin in this clinical syndrome may be explained by its antibacterial activity against Pasteurella species and Mycoplasma species. This field trial supports the in vitro studies which suggested than enrofloxacin is an appropriate therapy in cases of shipping fever. 相似文献
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OBJECTIVE: To compare pharmacokinetics of enrofloxacin administered IV and in various oral preparations to ewes. ANIMALS: 5 mature Katahdin ewes weighing 42 to 50 kg. PROCEDURE: Ewes received 4 single-dose treatments of enrofloxacin in a nonrandomized crossover design followed by a multiple-dose oral regimen. Single-dose treatments consisted of an IV bolus of enrofloxacin (5 mg/kg), an oral drench (10 mg/kg) made from crushed enrofloxacin tablets, oral administration in feed (10 mg/kg; mixture of crushed enrofloxacin tablets and grain), and another type of oral administration in feed (10 mg/kg; mixture of enrofloxacin solution and grain). The multiple-dose regimen consisted of feeding a mixture of enrofloxacin solution and grain (10 mg/kg, q 24 h, for 7 days). Plasma concentrations of enrofloxacin and ciprofloxacin were measured by use of high-performance liquid chromatography. RESULTS: Harmonic mean half-life for oral administration was 14.80, 10.80, and 13.07 hours, respectively, for the oral drench, crushed tablets in grain, and enrofloxacin solution in grain. Oral bioavailability for the oral drench, crushed tablets in grain, and enrofloxacin in grain was 4789, 98.07, and 94.60%, respectively, and median maximum concentration (Cmax) was 1.61, 2.69, and 2.26 microg/ml, respectively. Median Cmax of the multiple-dose regimen was 2.99 microg/ml. CONCLUSIONS AND CLINICAL RELEVANCE: Enrofloxacin administered orally to sheep has a prolonged half-life and high oral bioavailability. Oral administration at 10 mg/kg, q 24 h, was sufficient to achieve a plasma concentration of 8 to 10 times the minimum inhibitory concentration (MIC) of any microorganism with an MIC < or = 0.29 microg/ml. 相似文献
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氧氟沙星对霉形体与大肠杆菌合并感染鸡的药效学研究 总被引：6，自引：0，他引：6
以试管两倍稀释法测得氧氟沙星及其对照药物恩诺沙星、洛美沙星和强力霉素对鸡败血霉形体的最小抑菌浓度分别为０．００６２５、０．０２５、０．４和０．４ｍｇ／Ｌ。５０、１００、２００ｍｇ／Ｌ氧氟沙星、５０ｍｇ／Ｌ恩诺沙星、５０ｍｇ／Ｌ洛美沙星和１００ｍｇ／Ｌ强力霉素连续５ｄ饮水给药,对人工合并感染败血霉形体和大肠杆菌病鸡的治愈率分别是９３．３％、９６．７％、９６．７％、１００％、９６．７％和８３．３％,感 相似文献
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试验基于饲粮中添加不同水平维生素D3对种鹅生产繁殖性能、血清激素等的研究,进一步探索其对种鹅蛋品质、血液生化指标、二者相关性及蛋壳超微结构的影响。选择体况相近的34周龄五龙鹅种鹅180只,随机分为6组,每组6个重复,每重复1只公鹅和4只母鹅。6组的维生素D3添加量分别为0、100、200、300、400、500 IU/kg。预试期1周,正试期10周。结果表明:0 IU/kg组蛋壳强度低于其他各组(P<0.01);400、500IU/kg组蛋壳厚度厚于0、100IU/kg组(P<0.01);300、400IU/kg组血清钙含量高于0IU/kg组(P<0.05);200、300、400 IU/kg组血清磷含量高于0、500 IU/kg组(P<0.01);血清钙含量与蛋重和蛋壳强度、血清磷含量与蛋壳强度呈显著正相关关系;300、400、500 IU/kg组蛋壳乳突层厚度大于0、100 IU/kg组(P<0.01)。可见,适宜维生素D3水平可显著改善蛋壳乳突层厚度;血清钙磷含量与种蛋的蛋重、蛋壳强度... 相似文献