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1.
东农３０３（Ｓ．Ｔｕｂｅｒｏｓｕｍ）核型为：２＝４ｘ＝４８＝５×（４）＋５×（４）ＳＭ＋１×（４）ＳＴ＋１×［（２）ＳＴ＋（２）］，组型为：２ｎ＝４ｘ＝１１Ⅳ＋＋（１Ⅱ＋１Ⅱ）＝５Ｍ（Ｂ、Ｅ、Ｇ、Ｋ、Ｌ）＋５ＳＭ（Ａ、Ｃ、Ｄ、Ｈ、Ｊ）＋１ＳＴ（Ⅰ）＋１［（Ｔ＋ＳＴ）（Ｆ）］。Ｓ４－５－３－６－５－１－５０－（１０）（Ｓ．Ｔｕｂｅｒｏｓｕｍ）核型为：２ｎ＝４ｘ＝４８＝４×（４）Ｍ＋６×（４）ＳＭ＋１×（４）ＳＴ＋１×［（２）ＳＴ＋（２）Ｔ］，组型为：２ｎ＝４ｘ＝１１Ⅳ＋（１Ⅱ＋１Ⅱ）＝４Ｍ（Ｅ、Ｇ、Ｋ、Ｌ）＋６ＳＭ（Ａ、Ｂ、Ｃ，Ｄ、Ｈ、Ｊ）＋１ＳＴ（Ⅰ）＋１［（Ｔ＋ＳＴ）（Ｆ）］。Ａ组与Ｆ组带有髓体，两份供试材料的对应组中染色体略有分化，但基本一致。 相似文献
2.
在ＰＨ＝１．５（Ｈ２ＳＯ４）－０．１１ｍｏｌ／Ｌ Ｎａ２ＳＯ４－０．０４８ｍｏｌ／Ｌ苯羟乙酸－０．６３ｍｏｌ／Ｌ ＮａＣｌＯ３体系中，Ｍｏ（Ⅵ）可产生一灵敏的极谱催化波，峰电位为－０．２３Ｖ（ＶＳ．ＳＣＥ），线性范围为０．０４－１００ｎｇ／ｇ，用极谱催化波测定茶叶中痕量钼，兼具灵敏、准确、简便、选择性好的优点。 相似文献
3.
本文根据ＥｂｅｒｈａｒｔＲｕｓｓｅｌｌ法和ＴａｉＧ．Ｃ．Ｃ法设计了测定品种稳定性的程序，计算出Ｅｂｅｒｈａｒｔ－Ｒｕｓｓｅｌｌ法的１品种对环境的反应参数─回归系数ｂ１；２稳定性参数─离线性回归的方差Ｓ２ｄ和ＴａｉＧ．Ｃ．Ｃ法的１品种对环境的直线响应χ；２直线效应的离差λ。输入一次原始数据可计算出两种算法的品种稳定性参数。 相似文献
4.
橡胶树HMG-CoA还原酶基因结构序列分析 总被引：4，自引：0，他引：4
橡胶树ＨＭＧ－ＣＯＡ还原酶ｃＤＮＡ序列分析结果表明，该ｃＤＮＡ长段为１３８３ｂｐ，由此推导的氨基酸序列含有４６１个氨基酸残基。ＰＣＧＥＮＥ￣（ＴＭ）分析该ｃＤＮＡ克隆与拟南芥ＨＭＧ－ＣｏＡ还原酶ｃＤＮＡ序列同源性为７９．７％，氨基酸序列同源性为８０．２％，与苍鼠氨基酸序列同源性为５１％，与血吸虫氨基酸序列同源性为４８％。（１）发现ＨＭＧ－ｏｎＡ还原酶的一级结构在动物与植物之间存在较大的差异。（２）分析ＨＭＧ－ＣｏＡ还原酶的疏水性氨基酸图谱，发现橡胶树和拟南芥这两种植物权有１个跨膜势能区域（Ｄｏｍａｉｎ），而血吸虫、苍鼠、果蝇等几种动物却有７个跨膜势能区域，这说明该酶的二级结构在动植物之间也存在着较大的差异。（３）由于所分析的几种动植物ＨＭＧ－ＣｏＡ还原酶的羧基一端未见有疏水性区域，故推测具有疏水性区域Ｎ－端蛋白质与膜相结合，而酶的羧基端由于具有亲水性则起到酶的催化中心位点。（４）比较橡胶树ＨＭＧ－ＣｏＡ还原酶和拟南芥ＨＭＧ－ＣｏＡ还原酶氨基酸同源性，发现蛋白质（酶）的Ｎ－端氨基酸同源性较低，而靠近Ｃ－端同源性较高，这说明Ｃ－端部分较为保守，估计与酶的活性中心区域有关，而Ｎ－端同源性较差，估计跟酶与结合的膜不 相似文献
5.
适制乌龙茶品种茶树田间光合特性 总被引：9，自引：0，他引：9
采用ｃｌ－３０１ＣＯ２气体分析仪,以福鼎大白茶为对照,对６个适制乌龙茶品种壮年茶树的单叶光合特性,进行田间不离体测定。结果表明：乌龙茶品种茶树平均净光合速率为７．６６μｍｏｌｍ－２ｓ－１;它与光饱和点１５５３．５μｍｏｌｍ－２ｓ－１、光补偿点４７．１μｍｏｌｍ－２ｓ－１、胞间ＣＯ２浓度１６４．３×１０－６均呈负相关。其中,三倍体良种——梅占的净光合速率最高,而光饱和点、光补偿点和胞间ＣＯ２浓度最低,可选为高光效育种的亲本。 相似文献
6.
９６１１０巴西－黄粒Ｔｕｘｐｅｎｏ品种衍生的玉米单交种的杂种优势──（Ｅ．Ｅ．ＣｅＧａｍａ等），ＲｅｖｉｓｔａＢｒａｓｉｌｅｉｒａｄｅＧｅｎｅｔｉｃａ，１９９５，Ｖｏｌ．１８，Ｎｏ．１，８１～８５（英文）在热带地区多数杂交种使用Ｔｕｘｐｏｎｏ马齿型×... 相似文献
7.
茶儿茶素类和生物碱成分的HPLC分析 总被引：9，自引：0，他引：9
建立了以恒组成溶液为流动相的ＨＰＬＣ同时分析茶中５种儿茶素类（Ｃ、ＥＣＧ、ＥＣ、ＥＧＣ、ＥＧＣＧ）和３种生物碱（Ｃａｆ、Ｔｐ、Ｔｂ）的定量方法，色谱条件为：ＯＤＳ（１０μｍ）柱，以０．０４ｍｏｌ·Ｌ柠檬酸水溶液─Ｎ，Ｎ─二甲基甲酰胺─四氢呋喃（９０：１６：４：）为流动相，ＵＶ检测器（２７８ｎｍ），以二氯磺胺为内标。讨论了这些成分的提取方法和分离测定条件，此法分离效果好，操作简便，重现性好，分析结果准确。 相似文献
8.
对２３个水稻（Ｏｒｙｚａ Ｓａｔｉｖａ Ｌ．）核雄性不育（ｍｓ）突变体的花粉特征、不育性遗传和不育基因等位性 进行了研究。 １０个突变体由乙基甲烷磺酸（ｅｔｈｙｌ ｍｅｔｈａｎｅ ｓｕｌｆｏｎａｔｅ）诱变而来，１个由亚乙基亚胺 （ｅｔｈｙｌｅｎｅｉｍｉｎｅ）引变，７个通过ｒ射线诱变，１个被链霉素（ｓｔｒｅｐｔｏｍｙｃｉｎ）引变，１个通过组织培养而成，还有３个 大田中自发突变产生。在这些ｍｓ突变体中观察到４种花粉败育类型；３个属无花粉型（ＮＰ），６个为花粉完全败 育型（ＣＰＡ），１３个属花粉部分败育型（ＰＰＡ）和１个为花粉染败型（ＳＰＡ）。 ２年的后代测定（Ｐｒｏｇｅｎｙ ｔｅｓｔｓ）表明每种突变体都是一对隐性基因遗传。对 ２３个ｍｓ。突变体的不完全双列 杂交试验表明，突变体Ｅ２与Ｇ４等位，Ｅ５与Ｅ９等位，Ｎ２和Ｎ３等位，其它３个突变体Ｅ２、Ｇ６和Ｔ１具有相同的 ｍｓ位点。这些突变体的基因符号已被命名为ｍｓ－４６（ｔ）～ｍｓ－６３（ｔ）。 相似文献
9.
大量元素对茶愈伤组织生长及儿茶素累积的影响 总被引：7，自引：1，他引：7
本文分别研究了培养基中氮源组成与浓度，以及Ｋ＋、Ｃａ２＋、Ｍｇ２＋、ＰＯ４３－和ＳＯ４２－对茶愈伤组织生长和儿茶素累积含量的影响。结果表明，全硝态氮对于愈伤组织儿茶素形成最为有利；而ＮＯ３－／ＮＨ４＋比为７：３时愈伤组织生长最快；总氮浓度为１５ｍｍｏｌ／Ｌ时愈伤组织生长量最高；提高氮浓度则导致儿茶素含量急剧下降。添加Ｋ＋促进愈伤组织生长并使儿茶素含量降低；而１ｍｍｏｌ／Ｌ的Ｃａ２＋对儿茶素形成最为有利。在其余的离子中，ＰＯ４３－对愈伤组织生长影响较大，而ＳＯ４２－对儿茶素形成影响较大，Ｍｇ２＋浓度对两者均无显著影响。所得结果为采用两步法体系培养茶树细胞、系统研究儿茶素类物质的形成奠定了基础。 相似文献
10.
植物离体培养物中鱼藤酮的分离与HPLC测定 总被引：3，自引：0，他引：3
毛鱼藤（Ｄｅｒｒｉｓ ｅｌｌｉｐｔｉｃａ （Ｒｏｘｂ．）Ｂｅｎｔｈ．）和西非灰毛豆（Ｔｅｐｈｒｏｓｉａ ｖｏｇｅｌｉｉ Ｈｏｏｋ．ｆ．）离体培养物经９：１ＣＨＣｌ３／ＭｅＯＨ溶剂提取,Ｃ－１８柱层析分离后,在室温条件下以６６：３４ＭｅＯＨ／Ｈ２Ｏ或４２：５８ＣＨ３ＣＮ／Ｈ２Ｏ为流动相,支样品进行反相柱ＨＰＬＣ分析。结果表明,毛鱼藤和西百灰毛豆的愈伤组织均能合成鱼藤酮,但含量相当低,分别为１７．４μｇ／ｇ,１．８μｇ／ｇ干重。经诱导生根分化的毛鱼藤愈伤组织鱼藤酮含量达１３６．２μｇ／ｇ干重,为愈伤组织含量的７．８倍。西非灰毛豆悬浮培养物的鱼藤酮含量虽比愈伤组织有很大提高,但还不及毛鱼藤愈伤组织含量。结果表明,植物的基因类型在次生代谢上起重要作用,同时离体组织或细胞的分化有助于鱼藤酮的生物合成。 相似文献
11.
为给小麦品种镇麦168优质高产栽培中氮肥和种植密度管理提供参考,在基施有机肥15 000kg·hm-2、复合肥(N∶P∶K=15∶15∶15)375kg·hm-2条件下,设置240、285和330kg·hm-2三个追施氮肥水平以及135万、180万、225万、270万和330万株·hm-2五个种植密度(基本苗)水平,研究了追氮量和种植密度对该品种群体质量、产量和加工品质的影响。结果表明,在240~285kg·hm-2范围内,增加追氮量可明显提高镇麦168的籽粒产量、千粒重、成穗率、沉降值和弱化度,但当追氮量达到330kg·hm-2时,有效穗数、穗粒数、籽粒产量、容重、出粉率、吸水率、面团稳定时间增加不显著,千粒重明显降低。在135万~270万株·hm-2范围内,增加种植密度可显著提高有效穗数、籽粒产量、容重、面团形成时间、稳定时间和弱化度,而当种植密度达到330万株·hm-2时,有效穗数、穗粒数、千粒重、籽粒产量、成穗率、容重、面团形成时间、稳定时间和弱化度显著降低,分蘖期和拔节期群体叶面积指数、越冬期和拔节期干物质积累量以及出粉率、沉降值、吸水率增幅不明显。综合来看,镇麦168高产优质栽培的适宜追氮量和密度分别为285kg·hm-2和270万株·hm-2。 相似文献
12.
G. M. Arifuzzaman Khan Md. Shaheruzzaman M. H. Rahman S. M. Abdur Razzaque Md. Sakinul Islam Md. Shamsul Alam 《Fibers and Polymers》2009,10(1):65-70
The effect of chemical treatment i.e. bleaching, alkalization and graft copolymerization on the morphology changes of okra
bast fiber has been investigated by means of infrared spectroscopy (IR), scanning electron microscopy (SEM), water absorption
and tensile properties measurements. The graft copolymerization reaction of bleached fiber with acrylonitrile monomer (AN)
has been carried out under the catalytic influence of K2S2O8 and FeSO4 redox system. The maximum graft yield (11.43 %) has been found at 70°C temperature, 3×10−2 mol/l acrylonitrile, 5×10−3 mol/l K2S2O8, 5×10−3 mol/l FeSO4 and for 90 min. On the contrary, the fiber has been treated with 10 % NaOH solution, which is much effective to remove the
impurities. Based on findings of water absorption, tensile properties and SEM micrograph, the AN-grafted fiber has been showed
better properties than bleached and alkali treated fibers. The degree of modification of okra bast fiber by chemical treatment
has been evaluated by IR measurement. 相似文献
13.
解淀粉芽孢杆菌B9601-Y2提高玉米生长和产量的效应 总被引：10，自引：2，他引：8
采用促进植物生长的解淀粉芽孢杆菌B9601-Y2拌、浸玉米种子和浇灌土壤后均能显著提高玉米生长速度和生物量。3×101～3×105 cfu/mL浓度菌液浸泡玉米种子1 h,播种后5 d苗高比对照(清水)提高119.88%～168.85%;浸种2 h,3×104 cfu/mL和3×105 cfu/mL处理则有抑制作用。采用1.6×108、6.2×107、3.9×107 cfu/mL菌液浇灌土壤,播种后7 d苗重比对照增加20.75%、27.36%和33.96%。以3.9×107～1.6×108 cfu/mL浓度菌液拌种处理,15 d苗龄的植株重量比对照增加4.03%～29.35%,在收获期株高增加6.00%～16.29%,产量增加5.76%～11.81%。高浓度处理促生长效果不如中浓度处理。 相似文献
14.
为探究增密减氮栽培途径的可行性及其碳氮代谢调控机制,于2019-2020年以徐麦35为材料,设置不同密肥组合,研究增密减氮对产量和氮效率的调控作用。结果表明,适当减少施氮量,徐麦35的氮素农学效率（NAE）、氮肥偏生产力(PFP)、氮素生理效率（PE）、氮收获指数（NHI）均显著增加。开花期和成熟期的碳氮比（C/N）与产量、NAE、PFP、PE均呈显著正相关。密度240×10·hm-2~300×10·hm-2、施氮量180 kg·hm-2时产量可达到8 500 kg·hm-2以上,高于180×10·hm-2和270 kg·hm-2密肥组合下的产量水平。同时,NAE、PFP、氮肥吸收效率（RE）、和PE分别提高了62.1%~67.9%、57.6%~59.1%、5.1%~14.4%和41.6%~59.4%。返青期施用控释肥（A1）较拔节期施用尿素(A2)的氮肥吸收效率平均提高了6.8%,有利于实现江苏淮北麦区节本增效绿色轻简化栽培。 相似文献
15.
Using p-methylphenol as a model compound for phenolic hydroxyl groups on silk fibroin, the reaction kinetics of two typical of reactive dyes including vinyl sulfone reactive dyes and monochlorotriazine reactive dyes with phenolic hydroxyl groups were studied by reverse phase ion-pair high-performance liquid chromatography. The results showed that, the reaction rates of monochlorotriazine reactive dyes with phenolic hydroxyl groups were by far faster than those of vinyl sulfone reactive dyes, attributed to the stability of resultants from the former. The optimum reaction conditions for phenolic hydroxyl groups with vinyl sulfone reactive dyes and monochlorotriazine reactive dyes were at 50–70 °C and pH=8–9 for the former, and at 85–95 °C and pH=8–9 for the latter. The methods and results in this paper would help to deeply study the reaction mechanisms of reactive dyes with silk fibroin. 相似文献
16.
采用水蒸气蒸馏法提取木瓜籽精油(PSO),测定PSO的总抗氧化能力、羟基自由基和DPPH自由基清除能力,并应用GC-MS分析测定PSO的化学组成。结果表明,水蒸气蒸馏法提取木瓜籽精油最佳蒸馏时间是2.5 h,料液比和浸泡时间分别是1∶8(w∶V) 和1 h;与合成抗氧化剂相比,PSO的总抗氧化能力偏低,当PSO浓度达到5×10-5 mg/mL和8 mg/mL时,其清除羟基自由基和DPPH自由基的能力分别达到100%和70%以上;PSO化学成分主要是安息香醛、苯乙醛、苯乙腈和异硫氰酸苄酯,其中异硫氰酸苄酯的相对含量占98.28%。说明该方法可获得高纯度的异硫氰酸苄酯,可为工业上生产异硫氰酸苄酯提供一定的理论依据。 相似文献
17.
儿茶素是茶叶的主要功能成分,本试验探讨了乙醇浸提、硫酸-香荚兰素比色法检测绿茶面包中儿茶素含量的可行性.选择95％乙醇溶液作为提取溶剂,采用3因素3水平正交试验设计方案,考察料液比、浸提温度、浸提时间对绿茶面包中儿茶素总量提取效果的影响,并对硫酸-香荚兰素比色法检测绿茶面包中儿茶素含量进行了方法学考察.结果表明,绿茶面包中儿茶素总量检测的最佳浸提条件为:料液比1:20(g·mL-1),70℃水浴浸提45 min;硫酸-香荚兰素比色方法为:取0.5 mL浸提液,加入2.5 mL1％的香荚兰素/乙醇溶液和2.5 mL30％的硫酸/乙醇溶液,在20℃下反应10～30 min,取反应液在500 nm处测定吸光度.本方法中儿茶素浓度在0.025～0.3 mg·mL-1范围内,吸光度与浓度线性关系良好(R2=0.9996),同一样品重复测定6次的RSD为1.31％,儿茶素高、中、低浓度加标回收率范围在98.75％～103.25％,本方法满足绿茶面包中儿茶素总量的检测分析,儿茶素含量可作为标定绿茶面包中茶叶添加量的评价指标. 相似文献
18.
Preparation and Characterization of A Semi-interpenetrating Network Alkaline Anion Exchange Membrane
Yifu Wang Heting Wan Dan Wang Jilin Wang Lulu Wang Ruijiang Feng 《Fibers and Polymers》2018,19(1):11-21
A series of semi-interpenetrating network (semi-IPN) anion exchange membranes (QCS/St-G8-2-8, Quaternized chitosan/styrene-[maleic alkylene group diethyl bis (octyl dimethyl chloro/bromide), abbreviated as G8-2-8] were prepared via in-situ polymerization by Styrene (St) and G8-2-8 in QCS casting solution. During the process of in-situ polymerization, linear block polymers (St-G8-2-8) of Styrene and G8-2-8 was constructed, then was mixed with QCS casting solution, followed crosslinking the QCS by glutaraldehyde (GA). With the increasing content of linear block polymer, water uptake and swelling ratio of the composite membrane decreased; This kind of linear structure makes an order arrangement of quaternary ammonium groups which improves the OH? migration efficiency. At 70 °C, the M-30 composite membrane performs a high OH? conductivity of 8.20×10-2 S·cm-1, the methanol permeability is 3.23×10-6 cm-2·s-1 which is still lower than Nafion 115 of 2.42×10-6 cm-2·s-1, but M-30 shows a higher selectivity of 25.3 than Nafion 115 of 11.6. Furthermore, the membranes exhibited excellent thermal stability (≥150 °C), the tensile strength of the composite membrane is in the range of 14-25 MPa and elongation at break is in the range of 16-37 % at room temperature, as well as superior chemical stability in 1.0 M KOH solution for 250 h. 相似文献
19.
Fatemeh Zamani Mohammad Amani-Tehran Arash Zaminy Mohammad-Ali Shokrgozar 《Fibers and Polymers》2017,18(10):1874-1881
The complex nature of spinal cord injuries has provided much inspiration for the design of novel biomaterials and scaffolds which are capable of stimulating neural tissue repair strategies. Recently, conductive polymers have gained much attention for improving the nerve regeneration. In our previous study, a three-dimensional (3D) structure with reliable performance was achieved for electrospun scaffolds. The main purpose in the current study is formation of electrical excitable 3D scaffolds by appending polyaniline (PANI) to biocompatible polymers. In this paper, an attempt was made to develop conductive nanofibrous scaffolds, which can simultaneously present both electrical and topographical cues to cells. By using a proper 3D structure, two kinds of conductive scaffolds are compared with a non-conductive scaffold. The 3D nanofibrous core-sheath scaffolds, which are conductive, were prepared with nanorough sheath and aligned core. Two different sheath polymers, including poly(lactic-co-glycolic acid) PLGA and PLGA/PANI, with identical PCL/PANI cores were fabricated. Nanofibers of PCL and PLGA blends with PANI have fiber diameters of 234±60.8 nm and 770±166.6 nm, and conductivity of 3.17×10-5 S/cm and 4.29×10-5 S/cm, respectively. The cell proliferation evaluation of nerve cells on these two conductive scaffolds and previous non-conductive scaffolds (PLGA) indicate that the first conductive scaffold (PCL/ PANI-PLGA) could be more effective for nerve tissue regeneration. Locomotor scores of grafted animals by developed scaffolds showed significant performance of non-conductive 3D scaffolds. Moreover, the animal studies indicated the ability of two new types of conductive scaffolds as spinal cord regeneration candidates. 相似文献
20.
Textile industry generate significant amount of waste fibres in form of short lengths during mechanical processing. However these short fibres possess excellent properties suitable for many other applications. The objective of this work was to use them for the preparation of nanoparticles/nanofibres as fillers in biodegradable composite applications such as food packaging, agriculture mulch films, automotive plastics, etc. The present paper concerns with jute fibres as a source of nanocellulose for reinforcement of PVA mulch films. Jute fibres were first refined to micro/nanoscale particles in form of nanofibrillar cellulose (NFC) by high energy planetary ball milling process in dry and wet condition. Wet milling was observed more efficient than dry milling in terms of unimodality of size distribution with reduction in size below 500 nm after milling for 3 hours. Later the obtained particles were used as fillers in Poly vinyl alcohol (PVA) films and their reinforcement evaluated based on thermal properties. It was observed that glass transition temperature (Tg) of PVA films improved from 84.36 °C to 95.22 °C after addition of 5 % jute particles without affecting % crystallinity and melting temperature (T m) of PVA. Dynamic mechanical analysis of composite films with 5 % jute particles showed higher value of 14×108 Pa for storage modulus in comparison to 9×108 Pa of neat composite film. The percolation effect was observed more above glass transition temperature which consequently resulted in improved transfer of stiffness from jute particles to PVA matrix above 50 °C. The percolation phenomena also explained the improvement in thermal stability by 10 °C for every increased loading of jute particles due to formation of hydrogen bonds with PVA matrix. 相似文献