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1.
Blood from cockroaches stressed by having their mouthparts sealed or by being dehydrated by contact with silica aerogel, administered into the opened wounds of freshly excised honey bee abdomens, produced neuromuscular reaction that was monitored and recorded as characteristic myograms. When blood became too viscous or unobtainable during the terminal stages of stress, coxal muscle was substituted. Abdomens used for assay were also used for toxicological studies, comparing their survival time with untreated abdomens. Intact and decapitated cockroaches were equally desiccated by contact with silica aerogel to determine if presence or absence of the coordinating center had any bearing on stress. Fragments of coxal muscle from the intact cockroaches deactivated 10 bee abdomens in 15 hr while coxal muscle from the decapitated cockroaches deactivated only one out of 10 abdomens. Blood from cockroaches poisoned by oral administrations of boric acid solution was readily available, clear, and free-flowing, and produced myograms showing periods of lethargy followed by deep convulsions. Portions of these myograms resembled symptoms of lethargy and convulsions produced by boric acid solution when it was administered directly into the bee abdomens.  相似文献   

2.
Oral toxicity of boric acid, an inorganic insecticide, was evaluated on German cockroach, Blattella germanica L. (Dictyoptera, Blattellidae). Newly emerged adults were exposed to various concentrations of boric acid incorporated into the diet. Results showed that treated insects exhibited toxic symptoms with a dose-dependent mortality. Histological study of midgut revealed alterations in the epithelial cells and a significant increase in the epithelium thickness. In a second series of experiments, the compound was investigated on the activities of acetylcholinesterase (AChE) and glutathione S-transferases (GSTs). Data showed that the compound induced GSTs and reduced the activity of AChE. From this experiment, it may be concluded that ingested boric acid caused death of insects perhaps ultimately by starvation via alterations of the midgut. Additionally, the compound seemed to present a neurotoxic action as evidenced by the symptoms of poisoning and the reduction in AchE activity.  相似文献   

3.
Bacillus thuringiensis Berliner in combination with 0.1, 0.5, 1.0, 1.5 and 2% boric acid caused 100% mortality of the tobacco caterpillar,Spodoptera litura (F.), irrespective of instar, in a much shorter period (1.77 to 3.61 days) than with boric acid or the pathogen alone. The toxicity ofB. thuringiensis in combination with dicrotophos at 0.02 and 0.04%, with fenitrothion at 0.025 and 0.05%, or with dichlorvos at 0.01 and 0.05%, was enhanced.  相似文献   

4.
采用工蚁与浸药滤纸接触或取食含药松木粉的方法,测定了啶虫脒、氰戊菊酯和硼酸,在亚致死剂量下对黄胸散白蚁Reticulitermes flaviceps工蚁体内内切-β-1,4-葡聚糖酶(EG)和β-葡糖苷酶(BG)活性的影响。结果表明,用0.5、1.0和2.0 mg/L的啶虫脒处理黄胸散白蚁工蚁6~48 h,对其体内的EG和BG酶活性(处理48 h的除外)无显著影响;用0.5、1.0和2.0 mg/L的氰戊菊酯处理,可显著降低黄胸散白蚁工蚁体内的EG和BG酶活性;硼酸对黄胸散白蚁工蚁体内EG和BG酶活性的影响与处理浓度和处理时间有关。  相似文献   

5.
Natural occurring amorphous silica is ubiquitous on the earth in soils and sediments. Both, natural derived silica and synthetically produced amorphous silica are used against stored product pests and parasites in animal houses. New formulations, active even under higher relative humidity's can be possible used against horticultural arthropod pests. Since many horticultural pests hide on the undersides of leaves and silica is a physical contact insecticide, an appropriate application method is needed. This paper reviews electrostatic application of natural derived amorphous silica to Brassica chinensis leaves. Due to the inherent high volume resistivity of silica powder, induction charging was ineffective for electrostatic powder coating. Corona charging involves a reliable current source, but typically is inefficient in charging the powder particles and creates a high density of free ions between the gun and grounded substrate as well as within the coating layer. These factors resulted in poor coating quality and inefficient particle deposition. The best leave coating was archived using tribo charging. Here we have been able to apply coatings with different thickness. While a silica coating of 25–50?μm in petri dish experiments resulted in a reduced mortality of the granary weevil, Sitophilus granarius (L.), there was no difference between mortality with coatings of 50–100?μm and 100–125?μm. Silica applications on B. chinensis leaves resulted within two hours in a 45% reduction of the CO2-absorption. Absorption level stayed reduced over a period of seven days and was between 45 and 73% compared to the control. Even after removing silica dust using pressurized air CO2-absorption was between 19 and 28% reduced. This might be the case because silica particles where so small that they could possible clog the stomata opening resulting in a reduced transpiration and photosynthesis rate.  相似文献   

6.
Adult male German cockroaches were exposed to surface deposits of insecticides for 5 min in the central compartment of a three-compartment choice chamber illuminated at one end. They were then allowed access to the escape compartments and their distribution recorded over the subsequent 60 min. Cockroaches exposed to pyrethroid insecticides rapidly accumulated in the escape compartment closest to the light source; the same effect was observed to a lesser extent following exposure to propoxur. Boric acid and bendiocarb deposits inhibited the movement of the cockroaches away from the light source, and fenitrothion deposits delayed it. Dieldrin had no effect on the distribution of cockroaches within the choice chamber. The reversal of the normal tendency of German cockroaches to move from a light area to a dark harbourage following exposure to pyrethroids may contribute to the flushing action observed when these chemicals are used for control of B. germanica populations.  相似文献   

7.
Margosine-0®, a commercial preparation of neem seed extract, was tested for its effects as a toxicant, growth inhibitor, or repellent against six species of cockroaches —Blatta orientalis L.,Blattella germanica (L.),Byrsotria fumigata Guérin-Méneville,Gromphadorhina portentosa (Schaum),Periplaneta americana (L.), andSupella longipalpa (F.) (Orthoptera: Blaberidae, Blattidae, and Blattel-lidae). Last-instar nymphs of these species fed Lab-Chow® pellets impregnated with neem extract at a rate of 0.5 ml/pellet showed increased mortality and retarded development. All lst-instar nymphs ofB, orientalis, B. germanica andS. longipalpa died after consuming treated Lab-Chow pellets. Topical application of 2 μl of Margosine-0 to the abdomens of last-instarB. orientalis nymphs, as well as injection of 0.5 μl, resulted in reduction of growth and increased mortality. Placing lst-instarB. orientalis nymphs on a surface treated with the neem extract had no notable effect. In a choice test,P. americana adults immediately preferred the pellets treated with 0.5 ml of neem extract, but pint cardboard cartons treated with 1.5 ml extract repelled them.  相似文献   

8.
The purpose of this study was to evaluate oxidative stress and neurotoxic potential of organophosphorus (OP) insecticide diazinon in the sentinel freshwater fish, Oreochromis niloticus. Antioxidant and acetylcholinesterase (AChE) enzyme activities and malondialdehyde (MDA) and protein levels were measured spectrophotometrically in gill, kidney, alimentary tract, and muscle tissues of fish treated with sub-lethal diazinon concentrations for 1, 7, 15, and 30 days. Dose-dependent inhibitions of AChE were observed in all the experimental fish. On the contrary of alimentary tract, MDA levels were elevated in kidney and muscle and gill was not affected. AChE and MDA levels intercorrelated in kidney and muscle tissues. Diazinon had increased superoxide dismutase (SOD) activities in all the tissues, while kidney was the most affected tissue. Tissue-specific alterations were observed on catalase (CAT) and glutathione peroxidase (GPx) activities; however, the activities were not changed in gill and muscle tissues for GPx and in gill, muscle, and kidney tissues for CAT. Protein levels decreased in kidney, muscle, and alimentary tract, while increased in gill and alimentary tract in 15 days. With respect to these results, diazinon has oxidative and neurotoxic potentials in O. niloticus. Observed changes with diazinon treatment were generally tissue-specific and dose-dependent.  相似文献   

9.
The herbicide diclofop-methyl caused an early and pronounced inhibition of the incorporation of [14C]acetate into leaf lipids of the sensitive plant species maize (Zea may L.), wild oat (Avena fatua L.), and barnyardgrass (Echinochloa crus-galli L.). With an EC50 value of approximately 10?7M inhibition was already apparent 0.5–4 hr after herbicide application. The fatty acid biosynthesis of tolerant bean (Phaseolus vulgaris L.), sugar beet (Beta vulgaris L.), and soybean (Glycine max L.) was not affected, with one exception [wheat (Triticum aestivum L.) belongs to the more tolerant species]; the inhibition of fatty acid biosynthesis, however, was in the same order of magnitude as in sensitive plants. More detailed studies showed that in wheat a recovery from inhibition of fatty acid biosynthesis occurred. Four days after herbicide application (0.18 kg diclofop-methyl/ha) in wheat normal fatty acid biosynthesis was restored, whereas in sensitive maize a 60% inhibition was maintained over the whole experimental period (8 days). The results support the view that tolerance of wheat to diclofop-methyl is based on its inactivation in leaves, whereas the tolerance of dicotyledonous species may probably lie at the level of the site of action of diclofop-methyl. In experiments with intact leaves, the inhibition of fatty acid biosynthesis resulted in an enhanced flow of [14C]acetate into organic acids and amino acids. This effect, however, was not always reproducible in experiments with leaf pieces or isolated root tips.  相似文献   

10.
Abstract

The German cockroach, Blattella germanica L. (Dictyoptera: Blattellidae), an invasive pest of human habitats, is distributed throughout the world, except in Antarctica. They have developed resistance against chemical pesticides used for the management of their populations. Numerous essential oils and their constituents have been tested; however, the insecticidal activities of clove bud powder, oil, and their constituents have not yet been tested against the German cockroaches. Thus, in this study, clove bud powder, oil, and their major constituents, eugenol and eugenol acetate, were evaluated for their contact toxicity and repellancy against adult German cockroaches under laboratory conditions. The clove bud powder applied at 30?mg/cm2 killed 92% of German cockroaches at 6?hours after treatment (HAT). Similarly, clove bud oil, eugenol and eugenol acetate applied at 4.00?ml/cm2 provided 95%, 85%, and 87% German cockroach mortality at 24, 6, and 24 HAT, respectively. At 2.0?ml/cm2, clove bud oil repelled 80% of German cockroaches within 30?min. In contrast, eugenol and eugenol acetate repelled 85% at 1.0?ml/cm2 and 2.5?ml/cm2, respectively, at 0.5 HAT. Based on this study, clove bud powder, oil, eugenol, and eugenol acetate could be environmentally friendly tools for the management of German cockroaches.  相似文献   

11.
Cylic AMP (cAMP) and cyclic GMP (cGMP) concentrations in toxaphene-treated cockroaches, Leucophaea maderae (Fab.), increased in all tissues sampled, with the greatest increases occurring at 24 hr for cAMP and 168 hr for cGMP. Asymptomatic mice treated with 112 mg/kg of toxaphene were not significantly different from controls. Symptomatic mice showed increased cAMP in all tissues examined and an increase in testicle cGMP. The greatest increases in cAMP occurred during the more advanced stages of toxaphene poisoning.  相似文献   

12.
In order to clarify the action mechanism of fluazifop-butyl, an aryloxyphenoxypropionate (AOPP) herbicide in bristly starbur (Acanthospermum hispidum DC.), a unique fluazifop-butyl-susceptible broad-leaved weed, ethylene evolution and membrane lipid peroxidation in the plant seedlings were investigated. Foliar application of fluazifop-P-butyl induced ethylene evolution only from bristly starbur, but not from oat (Avena sativa L.), another fluazifop-butyl-susceptible species, and two tolerant species, pea (Pisum sativum L.) and hairy beggarticks (Bidens pilosa L.). The other AOPP herbicides, quizalofop-ethyl and fenoxaprop-ethyl, and a cyclohexanedione (CHD) herbicide, sethoxydim, did not enhance ethylene production from bristly starbur. This fluazifop-butyl-induced ethylene production in bristly starbur was completely suppressed by aminoethoxyvinylglycine (AVG), a 1-aminocyclopropane-1-carboxylic acid (ACC) synthase inhibitor, but not by p-chlorophenoxyisobutyric acid (PCIB), an anti-auxin compound, suggesting this evolved ethylene was not auxin-induced. Phytotoxic action by fluazifop-P-butyl (5 μM) in bristly starbur was reduced markedly by two lipid-soluble antioxidants, vitamin E, and ethoxyquin. The ethylene production from the plant was also inhibited by these two antioxidants. Content of malondialdehyde, an indicator of lipid peroxidation, increased only by fluazifop-P-butyl in bristly starbur seedlings but not in oat, and this increase was inhibited by ethoxyquin. These results strongly suggest that the primary site of action for fluazifop-butyl in bristly starbur is on the membranes and active oxygen species and/or free radicals are involved in peroxidation. Ethylene evolution is probably induced by these reactive oxygen species.  相似文献   

13.
Esterases in acetone powder preparations of milkweed bugs, cockroaches, houseflies, cabbage loopers, mealworms, and mouse liver hydrolyze the (+)-trans- and (+)-cis-isomers of resmethrin and tetramethrin but they do not hydrolyze S-bioallethrin. Homogenate fractions are less suitable than acetone powders for assaying the insect esterases due to interfering reactions or inhibitors. The milkweed bug, looper and mouse liver esterases cleave the trans-isomers more rapidly than the cis-isomers of resmethrin and tetramethrin but this isomer specificity is less prominent or not present with the other esterase sources. Pyrethroid-hydrolyzing esterases are much less active in insect than in mouse liver preparations. 1-Naphthyl N-propylcabamate is a more potent inhibitor than S,S,S-tributyl phosphorotrithioate for the insect esterases whereas the latter compound is more effective in inhibiting the mouse esterases. Both of these chemicals are noncompetitive inhibitors in each case suggesting that they carbamoylate and phosphorylate the detoxifying enzymes. Esterase inhibitors acting in the nmolar range may be useful synergists in species where pyrethroid detoxification by esterases limits the insecticidal action.  相似文献   

14.
Ethyl formate and ethanol in air, eg in fumigant studies, were readily detected by gas chromatography (GC) (flame ionisation). Residues in wheat, barley and sultanas were analysed by GC, after extraction in polar solvents (eg methanol, aqueous propanol). Both natural levels and levels resulting from fumigation with ethyl formate were measured. Formic acid was extracted from commodities with polar solvents (eg methanol, water) and analysed by GC after esterification. Solvent extracts of commodities were concentrated after addition of disodium hydrogen orthophosphate, and an aliquot added to acidified alcohols (several combinations of acids and alcohols were tested) in a sealed container. Formic acid esters were determined by GC, from headspace sampling over the esterification solvent. Esterification was faster with strong acids than with boric acid. However, esterification with boric acid/butanol gave the least interference of all tested methods. Product identity was confirmed by GC/mass spectrometry. High natural levels of formic acid, and low natural levels of ethyl formate and ethanol, presented problems in identifying residues arising from fumigation. These natural levels are relevant to food regulations for ethyl formate, especially those based on ‘total formic acid, free and combined’. Polar columns (eg FFAP, carbowax) were useful for measurement of formic acid esters, which eluted before the alcohols used for esterification or extraction, whereas elution followed the molecular mass on non-polar columns, such as GS-Q or DB-624. © 1999 Society of Chemical Industry  相似文献   

15.
Feeding stimulants (phagostimulants) increase the food uptake by the host insect larvae and thereby play a vital role in enhancing overall activity of insect viruses formulated as biopesticides. A local strain of granulovirus of Pieris brassicae (PbGV), a serious pest of cole crops worldwide, has shown potential as a biopesticide against this pest and with slight increase in its efficacy can be used successfully as a promising biocontrol agent in integrated pest management (IPM) programs of the pest. The addition of phagostimulants may enhance the efficacy but so far no specific phagostimulant for PbGV has been reported. In the present studies, some reported phagostimulants for insect viruses were tested for their impact on food utilization by host larvae and efficacy of the virus. The specified concentration of virus isolate was mixed with phagostimulants and laboratory studies were conducted to uncover their effect on consumption index (CI) and larval mortality. The studies revealed that phagostimulants, viz. boric acid and crude sugar, when mixed with PbGV, showed highest CI and larval mortality. Consumption index of 1.34, 1.49 and 1.43 mg/mg/day and corresponding larval mortality of 32.9%, 45.8% and 34.2% were recorded with the addition of 0.5% and 1% boric acid and 2% crude sugar, respectively, in PbGV. The leaf extracts of cabbage, cauliflower and broccoli at both the concentrations (1% and 2%) also enhanced the CI but were comparatively less effective than the boric acid and crude sugar. Field evaluation of PbGV alone and in combination with phagostimulants carried out at two geographically isolated locations, viz. Palampur (subtropical) and Sangla (temperate) on three cole crops (cabbage, cauliflower and broccoli) showed that boric acid was the most promising candidate for enhancing the PbGV efficacy under field conditions, followed by crude sugar.  相似文献   

16.
Female adult American cockroaches, Periplaneta americana L., showed definite age-dependent changes in levels of activity of the microsomal mixed-function oxidases. Cytochrome P-450 levels, EPN-detoxication, and p-nitroanisole O-demethylation activities were very low in young adult insects but increased steadily reaching a natural peak at about 100 days in fat body and at about 90 days in midgut and hindgut. The activities then declined rapidly reaching levels of young insects at about 130 to 140 days of age. NADPH-neotetrazolium-reductase activity was high in young insects, declined later in adult life, and returned to a peak at about 100 days.Injections of chlorcyclizine, a known microsomal enzyme inducer, significantly increased levels of cytochrome P-450, EPN-detoxication, p-nitroanisole O-demethylation, and NADPH-NT-reductase activities in young cockroaches. The drug injections were effective, however, only before the natural activity peak was reached. Beyond this point the injections had no inductive effect indicating that the microsomal oxidases in this insect are uninducible when normal enzyme levels are falling.NADPH-NT-reductase activity in male cockroaches, while being somewhat higher than in females, showed a similar age-dependent curve with the peak occurring at about 120 days.Age-dependent carbaryl resistance in male and female insects tended to follow levels of the microsomal oxidase activities. Fifty to 60-day-old insects, however, tended to be more resistant to the insecticide than microsomal enzyme levels would indicate.RNA levels of normal female insects showed age-dependent curves similar to those of the microsomal enzyme activities, being low in young adults and reaching a peak at about 100 days. Chlorcyclizine injections had little or no effect on total microsomal RNA levels.  相似文献   

17.
The phagostimulatory response of the larvae of the gypsy moth,Porthetria dispar, to ethanolic-aqueous extracts of the leaf powder of different host and non-host plants, was assayed in no-choice experiments using the Styropor® method.Quercus robur L. was the most active at all concentrations tested;Picea excelsa Link, was highly active only at 4%;Pyrus malus L. showed an intermediate activity at 4%; and very slight or no feeding occurred with the extracts ofPinus silvestris L.,Ilex aquifolium L. andTaxus baccata L. The phagodeterrent effect of thePinus silvestris andIlex aquifolium extracts was demonstrated by treating the lamellae with a mixture of each one of them with the highly phagostimulatory extract ofQ. robur. The following monoterpenes tested were highly phagodeterrent at 0.2%: α-pinene, β-pinene, 3-carene; camphene was totally deterrent even at 0.05%, whereas limonene was not phagodeterrent even at 0.2%. Among other substances coumarin and lactic acid were active at 0.2%, whereas citric acid, oxalic acid and tannic acid were not phagodeterrent even at 0.2%.  相似文献   

18.
Changes in soluble proteins synthesized in soybean (Glycine max L.), safflower (Carthamus tinctorius L.), radish (Raphanus sativus L.), and barley (Hordeum vulgare L.) treated with either growth promotive or inhibitory concentrations of picloram (4-amino-3,5,6-trichloropicolinic acid) were determined by polyacrylamide gel electrophoresis and isoelectric focusing. A special gel mixture was developed which provided resolution of protein bands superior to that obtained by standard gel electrophoresis. Growth promotive concentrations of picloram caused both qualitative and quantitative alterations in the band patterns of soluble proteins of safflower, radish, and barley roots and shoots. Isoelectric focusing was applied for the separation and identification of soluble protein fractions from soybean and barley roots and shoots treated primarily with growth inhibitory concentrations of picloram (except for barley shoot tissues). More than 35 clear bands were distinguishable in a typical gel electrophoretogram for either soybean or barley tissue (4-day-old plants). Approximate pI values of the bands from barley root protein were determined from a pH gradient diagram. Protein band patterns of picloram-treated samples were changed qualitatively and quantitatively, in comparison with controls, mostly in the range above pI 6, and predominantly in the neutral and basic protein regions. Band patterns for 96-hr root samples treated with growth inhibitory concentrations of picloram were more similar to those from 48-hr (soybean) or 55-hr (barley) than 96-hr control seedlings. A quantitative decrease in intensity of a band which had the same pI value as that of RNase was noticed in both the treated samples and 2- or 3-day-old control seedlings.  相似文献   

19.
The biological effects of two important medicinal plants, Artemisia annua L. and Achillea millefolium (L.) (viz, mortality, growth, and feeding indices as well as enzyme and non-enzymatic activities) were studied on small white Pieris rapae L a deleterious pest of cruciferous plants under controlled conditions (16:8 h L:D at 25 ± 1 °C and 65 ± 5% RH). The LC50 and LC25 values were 9.387% and 3.645% for A. annua L. and 4.19% and 1.69% for A. millefolium (L.), respectively. At the lowest concentration (0.625%), the deterrency was 29.826% and 44.185% for A. annua L. and A. millefolium (L.), respectively. Feeding indices were variously affected with changes in a number of parameters and an increase in larval and pupal duration. The activity level of alkaline phosphatase increased sharply while alanin and aspartate aminotransferases showed a sharp decrease. For non-enzymatic compounds, the amount of glucose and uric acid increased, but total protein and cholesterol decreased. These results indicate that these two medicinal plants might possess potential secondary metabolites that may be useful for controlling potential insect pests.  相似文献   

20.
This work aimed at identifying plant compounds with insecticidal activity against Diaphania hyalinata (L.) (Lepidoptera: Pyralidae), Musca domestica (L.) (Diptera: Muscidae), Periplaneta americana (L.) (Blattodea: Blattidae) and Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae). The plant species used were: basil (Ocimum selloi Benth.), rue (Ruta graveolens L.), lion's ear (Leonotis nepetaefolia L.), Jimson weed (Datura stramonium L.), 'baleeira' herb (Cordia verbenaceae L.), mint (Mentha piperita L.), wild balsam apple (Mormodica charantia L.) and billy goat weed (Ageratum conyzoides L.). Firstly, the insecticidal activities of hexane and ethanol plant extracts were evaluated against adults of R. dominica. Among them, only the hexane extract of A. conyzoides showed insecticidal activity. The hexane extract of this plant species was therefore fractionated by silica gel column chromatography to isolate and purify its bioactive chemical constituents. Three compounds were identified using IR spectra, (1)H NMR, (13)C NMR, HMBC and NOE after gel chromatography: 5,6,7,8,3', 4', 5'-heptamethoxyflavone, 5,6,7,8,3'-pentamethoxy-4', 5'-methylenedioxyflavone and coumarin. The complete assignment of (13)C NMR to 5,6,7,8,3'-pentamethoxy-4', 5'-methylenedioxyflavone was successfully made for the first time. 5,6,7,8,3'-Pentamethoxy-4', 5'-methylenedioxyflavone did not show any insecticidal activity against the four insect species tested. 5,6,7,8,3', 4', 5'-Heptamethoxyflavone showed low activity against D. hyalinata and R. dominica and was not toxic to M. domestica or P. americana. In contrast, coumarin showed insecticidal activity against all four insect pest species tested, with the following order of susceptibility: R. dominica < P. americana < D. hyalinata < M. domestica after 24 h exposure.  相似文献   

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