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1.
棉花核不育系豫98-8A育性遗传分析 总被引：1，自引：1，他引：0
为了阐明1999年从转基因后代遗传群体中发现的1株雄性不育植株不育基因的遗传规律及其与现有不育基因的等位性,采用表型观察测量,以及经典的自交和测交手段,研究了该不育材料败育性状的遗传规律。花器官形态特征调查表明：不育株花柱长和花柱外露长度均明显高于同质系的正常可育株,而每朵花的子房直径及花药数量没有明显差异。遗传分析表明：杂合体可育株自交,后代不育株与可育株呈3:1分离,不育株与杂合姊妹可育株测交,不育株与可育株呈1:1分离,表明该核不育材料受隐性单位点控制;与阆A(msc1)、洞A(msc3)等育性位点杂合可育株分别杂交,其F1代单株育性均得到恢复。由其F1代产生的F1:2家系中均出现不育株与可育株呈1:3和7:9两个育性分离群体,表明该材料败育基因为不同于阆A、洞A的不育基因位点。 相似文献
2.
水稻显性雄性核不育基因鉴定初报 总被引：18，自引：4，他引：18
1978年我所用栽野型组合(萍矮58×华野)F_2中的无花粉型不育株与反交组合(华野×萍矮58)F_4中的正常株杂交,后代出现典败型变异株。经13个世代观察,该不育材料的测交、回交、姊妹交(不育株×可育株)F_1分离出的不育株与可育株呈1∶1;可育株自交后代育性不分离;“不育株”幼穗分化期在高温下(白天平均温度30℃以上)有部分结实, 相似文献
3.
发掘水稻新型雄性不育细胞质源CMS-FA,育成系列优质米不育系和系列新质源恢复系,组配成强优势杂交稻组合的基础上研究新质源雄性不育恢复系的恢复基因遗传。采用新质源(CMS-FA)不育系金农1A与恢复系金恢3号杂交获得杂交F1代种子,种植F1代,收获自交F2代种子。用F1分别与不育系或保持系回交,获得(不育系//不育系/恢复系和不育系/恢复系//保持系)2个测交群体。同时种植P1、P2、F1、F2、B1F1和B2F1等群体,考察花粉染色率、套袋结实率和自然结实率,卡平方测验遗传分离适合度。结果表明,不育系与恢复系杂交F1代正常可育,育性恢复(可育)基因为显性遗传。F2代分离出可育︰不育适合3︰1,育性恢复(可育)基因为1对显性基因控制。B1F1和B2F1代2个测交群体的可育︰不育都适合1︰1分离规律,验证了F2代育性恢复(可育)单基因的遗传模式。暂时确定新质源(CMS-FA)核质互作三系的基因型为不育系S(SS)、保持系F(SS)和恢复系S(FF)。 相似文献
4.
为了组配双低高油高产强优势杂交油菜组合,选育双低高油丰产性好的隐性核不育系.用隐性核不育杂交组合杂291的F2群体分离的不育株与双低高油丰产性好的R-9品系杂交、自交、连续5代兄妹交(不育株/可育株),转育成新的隐性核不育两型系NAB-2.在不匀苗的条件下,调查NAB-2群体不育株与可育株的比例1∶1,卡方检验不显著;可育株自交群体中,可育株与不育株的比例3∶1,卡方检验不显著;NAB-2芥酸0.5％、饼含硫代葡萄糖甙(以下简称硫甙)19.2,μmol/g、含油率45.9％;植物学性状和经济性状基本稳定、生育期230 d左右. 相似文献
5.
以自育的甘蓝型油菜双低细胞核隐性核不育材料98-116 A为母本与甘蓝型油菜凸耳双低品系T2632为杂交父本进行杂交转育,在F1可育株自交的同时进行去雄与杂交父本进行正反交,在杂交后代中选取生长健壮具有凸耳性状的可育株自交,并调查自交后代中的育性分离比例,自交4个世代后进行兄妹交,即获得双低的凸耳甘蓝型油菜隐性细胞核雄性不育系,该不育系的不育株率远高于98-116 A,其不育株率达到90%以上,而且其育性遗传恢复机理也发生了改变.该不育系在油菜隐性核不育两系杂优育种的研究与利用中具有重大的研究价值和应用前景. 相似文献
6.
粱全1504A是贵州粱丰农业科技有限公司以引进甘蓝型油菜杂交种核优202杂交种后代可育株连续自交、测交、兄妹交育成纯合两型系(粱纯4A),与引进甘蓝型油菜杂交种核优202杂交种后代不育株和从贵州省油菜所引进隐性上位互作核不育黄籽临保系(S2375C-15)杂交转育的临保系(粱临4C)组配的甘蓝型油菜隐性上位互作核不育系,该不育系综合性状优良,株型紧凑,含油量较高,一般配合力较高,组配的隐性上位互作核不育杂交油菜组合丰产性好,抗性好,品质性状优良,有广泛的应用前景。 相似文献
7.
食荚豌豆雄性不育突变体的遗传研究 总被引：1，自引：1，他引：0
对国内首例豌豆雄性不育突变体的不育度、遗传特点及稳定性进行研究。观察发现：在生育前期。不育株外部形态特征与正常株没有明显差异;现蕾后,剥开花蕾可看到不育株的花药呈淡黄色半透明状。而可育株的花药呈橙黄色。用I2-KI染色法镜检花粉的可染性,发现不育株的花药内没有花粉粒,败育彻底,为典型的“无花粉型”雄性不育。用不育株作母本,与同品系的正常可育株进行姊妹交,F1全部可育,F2可育株与不育株的分离比例为3：1。用不育株作母本,其他品系作父本进行测交,同时用其他品系作母本,姊妹交F1作父本进行反交,正反交后代的育性表现一致。F1全部可育。F2可育株与不育株呈3：1分离。结果表明：该雄性不育突变体的不育性是可遗传的,属单隐性基因控制的核不育类型,与细胞质遗传物质无关。在不同年份、不同季节下,不育性状表现稳定。 相似文献
8.
9.
甘蓝型油菜显性核不育三系材料遗传规律及其稳定性验证 总被引：2，自引：1，他引：1
甘蓝型油菜显性核不育两型系分为杂合两型系和纯合两型系,两型系内兄妹交后代育性保持１：１分离,双隐性保持系（临保系）,可使纯合两型系内不育株后代达到全不育,而用于制种,同时从杂交种后代的育性分离比例可以验证恢复系的基因型。 相似文献
10.
11.
A male sterile line with dominant gene (Ms) in cabbage (Brassica oleracea var. capitata) and its utilization for hybrid seed production 总被引：4，自引：0，他引：4
Zhiyuan Fang Peitian Sun Yumei Liu Limei Yang Xiaowu Wang Anfu Hou Chunsong Bian 《Euphytica》1997,97(3):265-268
A male sterile plant, 79-399-3, was identified from a spring cabbage line 79-399 in 1979. Light-microscopic studies with paraffin
section indicated that meiotic division stopped at the tetrad stage. The ratio of male sterile plants to fertile plants in
the progenies of test crosses was 1:1 in five experiments conducted in 1982 and 1991–1994. However, some male sterile plants
were sensitive and developed a very low number of viable pollen grains. When sensitive male sterile plants were selfed, the
progenies segregated into male sterile and fertile plants at a ratio of 3:1. The male sterile plants from selfing of sensitive
male sterile plants were propagated by tissue culture and crossed with different inbred lines. From the progenies of the crosses,
populations with 100% male sterile plants were observed. The results indicated that homozygous dominant male sterile (MsMs)
plants segregated from the selfed progenies. Populations with 100% male sterile plants and stable male sterility were developed
as male sterile lines. Several favorable combinations were also selected by crossing the male sterile lines with inbred lines
with high combining ability for desirable horticultural characters.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
12.
中国首例燕麦雄性不育的发现及遗传鉴定 总被引：7，自引：0，他引：7
对1994年发现的我国首例燕麦雄性不育材料进行了特征特性的观察和细胞学鉴定、以及不育性遗传的研究,结果表明:(1)该材料不育度为100%,属“无花粉型”的雄性不育,不育株小抱子败育发生在四分体形成后期到花粉粒形成早期阶段。(2)不育株与不同品种测交的F1代,6个组合表现育性恢复,2个组合出现一些完全不育株;恢复育性的植 相似文献
13.
Summary The male sterility system MS-1 of Brassica oleracea was studied in order to elucidate if nucleo-cytoplasmic interactions determine this system. Crosses of male sterile MS-1 genotypes with heterozygous MS-5 genotypes gave fully fertile F1 progenies. Selfing of seven F1 plants resulted in five F2 populations showing a 9:7 segregation ratio and two a 3:1 ratio for fertile and male sterile plants. Two F2 progenies deviated from the expected 9:7 or 3:1 segregation ratios for fertile and male sterile plants. Thermosensitivity and distortion of the meiosis are suggested as the causal factors underlying the deviation of the segregation ratios. It was concluded that nuclear factors determine the male sterility in the MS-1 system, because the presence of a nucleocytoplasmic interaction in this system should have given only a 3:1 segregation ratio for fertile and male sterile plants in the F2 generation. 相似文献
14.
Summary A monogenic dominant male sterility is used for hybrid production in autumn and winter cauliflower. The ratio of male sterile plants in the backcross progenies of autumn cauliflower was 1:1 over five years (1987–1991). However, a significant deficit of male sterile plants was observed in the winter type over the same period.The influence of the temperature on the male sterile phenotype was studied within backcross progenies planted inside polythene tunnels. Six classes of phenotype were defined during the flowering period (from May to November). At low temperature, some male sterile plants developed partial to complete male fertility, whereas at high temperature, male fertile plants became male sterile.Segregation among the progenies of self-pollinated unstable male sterile plants did not deviate from the expected 3:1 ratio. Plants homozygous for the male sterility allele have been revealed by test crosses with a male fertile plant.For use in seed production, stable male sterile plants are vegetatively maintained; however, crossing lines isogenic except at the MS locus would allow male sterile plants to be raised from seed. 相似文献
15.
Yang Zhuping 《Euphytica》1997,94(1):93-99
The fertility segregations of F1, F2, BCF1 descended from crosses between PSGMR and japonica varieties, and F1's anther cultured homozygous diploid pollen plant populations (H2) were studied to reveal the genetic mechanism of photoperiod sensitive genic male sterility in PSGMR under natural daylight
length at Shanghai. Rate of bagged seed-setting was used as an indicator of fertility. Fifteen F1 showed complete fertility similar to their parents. The ratio of completely sterile plants to fertile plants in fifteen F2 and four BCF1 was 1:15 and 1:3, respectively. The ratio of completely sterile to fertile diploid pollen plants in nine diploid populations
(H2) was 1:3. These results demonstrated that the photoperiod sensitive genic male sterility in PSGMR was governed by two pairs
of independent major recessive genes. There were no significant fertility segregations in hybrids F1 and selfed F2 between Nongken 58S and its derivatives 7001S, 5088S, 5047S and M105-9S, indicating that the photoperiod sensitive genic
male-sterile genes in Nongken 58S were allelic to those in its derivatives. Several photoperiod sensitive genic male-sterile
diploid pollen lines were bred from anther cultured homozygous diploid populations (H2) in about a three-year period. Most of these diploid lines showed significant fertility transformation and stable complete
sterility from 5 August to 5 September, excellent agronomic traits and high resistance to blast and bacterial leaf blight.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
16.
Ya-Jun Xi Xue-Feng Ma Huan Zhong Shu-Dong Liu Zhu-Lin Wang Yang-Yang Song Cheng-Hui Zhao 《Euphytica》2011,177(2):241-251
A male sterile plant of wheat (Triticum aestivum L.) segregated from progenies of a transgenic family containing the leaf senescence-inhibition gene P SAG12 -IPT in the genetic background of ??Xinong 1376??, a well adapted winter wheat cultivar. The male sterile plant (named TR1376A) showed no phenotypic changes, except for florets and male organs, compared to its male fertile sibling plants (named TR1376B). The glumes and florets of male sterile TR1376A plants widely opened whereas those of the fertile counterpart TR1376B were closed or opened only briefly at flowing. Anthers of TR1376A were slender and indehiscent, and failed to release pollen. Compared to TR1376B, TR1376A anthers contained greatly reduced amounts of pollen, which was inviable or weakly viable. Ultra-structure studies indicated that cells in the endothecium and middle layers of the anther wall were dissolved or poorly developed in the sterile anthers of TR1376A. Molecular studies showed that the male sterility of TR1376A was caused by a sequence deletion or mutation that occurred in the promoter region of the transgene. F1 hybrids of TR1376A and TR1376B gave 1:1 segregation of male fertility to sterility, indicating that the male sterility of TR1376A was heritable and controlled by a single dominant gene (named Ms1376). To date, only a few dominant nuclear male sterility genes have been characterized and one of them (Ms2) has been successfully used to improve wheat cultivars through recurrent breeding strategies. The discovery of the Ms1376 gene provides another dominant male sterile source for establishing recurrent breeding systems in wheat. 相似文献
17.
Digenic nature of male sterility in pepper (Capsicum annuum L.) 总被引：1，自引：0，他引：1
Summary A cross was made between two nearly isogenic lines differing for male sterility genes, viz. ms1ms1Ms2Ms2 s Ms1Ms1Ms2ms2. F1 plants yielded F2 populations which segregated either in 3:1 or 9:7 ratios of fertile vs male sterile individuals. Test crosses between male sterile and male fertile sibs in the 9:7 segregating populations provided a few lines in which most of the progenies were male sterile. A 3:1 ratio model of male steriles vs fertiles is suggested and the value of the system is discussed.Contribution A.R.O. Agricultural Research Organization, The Volcani Center, Bet Dagan 50 250, Israel No. 3703-E, 1992 series. 相似文献
18.
Zhi-Wei Wang Lei Gao Hai Zhou Liu Shi Yong Mei Yuan Zhou Chang Ping Xiang Ting Wang 《Euphytica》2012,186(2):313-320
A male sterile plant appeared in the radish breeding program at the Hubei Academy of Agricultural Sciences, Hubei, China.
In its progeny, a two-type (half of plants male sterile, the other half male fertile) line 01GAB was established. An F2 population of 260 plants from a cross of male-sterile 01GAB and a male fertile line 9802H segregated for male fertility in
a 3:1 ratio indicating that fertility was restored by a single dominant gene, here designated RsMs. A PCR-based DNA marker specific to the male fertility Rfob gene in 9802H was absent in 01GAB. Linkage analysis placed the RsMs locus 10.7 cM away from the Rfo locus. In an F2 population of hybrids between 01GAB and male fertile 9802B, a co-dominant DNA marker for the RSultr3.2A (a radish sulfate transporter gene) locus was linked to the RsMs locus at 1.5 cM suggesting that fertility restoration in 01GAB was located in the region with known male sterility restorers
in radish. However, no maintainer for the 01GAB source of male sterility has been identified so far. Cytological observations
have shown that the abnormalities in male sterile anthers first appeared in tapetum at the tetrad stage, followed by a hypertrophy
of the tapetal cells at the vacuolate microspore period. These results suggest that male sterility in 01GAB is likely to be
genetic in nature, or it may represent a new type of the cytoplasmic male sterility. 相似文献
19.
The male sterile plants that segregated in a BC5F2 of `C. sericeus × C. cajan var. TT-5' population were maintained by sib mating. The male sterile plants were crossed with ICPL-85012.Approximately 50%
of the F1 plants were sterile. F2 plants derived from the fertile F1 plants did not segregate for male sterility. The reciprocal hybrid i.e. ICPL-85012 × Fertile derivatives from C. sericeus × TT-5, did not express male sterility. However, among the 12 F2 plant to row progenies, two segregated 25% male sterile plants and remaining 10 did not segregate. The segregation pattern
in subsequent progenies revealed that the sterility was under control of a single recessive allele. Studies on the backcross
and their BC1F2 and BC1F3progenies revealed another sterility gene which was found to be dominant in inheritance. This paper shows that what was thought
to be cytoplasmic male sterility from C. sericeus cytoplasm is actually a single dominant gene possibly acting in concert with a single recessive gene to mimic cytoplasmic
male sterility.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献