共查询到19条相似文献，搜索用时 218 毫秒
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以甜瓜品种“黄醉仙”下胚轴为外植体,研究了添加不同浓度的BA和IAA激素组合对其愈伤组织、不定芽的诱导和不定芽伸长的影响,同时研究了添加不同浓度IBA激素组合对其生根的影响.结果表明:甜瓜“黄醉仙”下胚轴诱导愈伤组织最适宜培养基是MS+BA1.0 mg/L+IAA 0.5 mg/L和MS+BA 4.0 mg/L+IAA 1.0 mg/L,最适宜不定芽诱导的培养基是MS+BA 2.0 mg/L,不定芽伸长的最适宜培养基是MS+IAA 1.0 mg/L,而生根的适宜培养基是1/2MS+IBA 1.0 mg/L. 相似文献
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以菊花的叶片为外植体,在MS+6-BA 2.0 mg/L+NAA 1.0 mg/L培养基诱导叶片产生愈伤组织,并进行丛生芽的继代培养,研究6-BA和NAA不同配比对愈伤组织丛生芽的分化及不同浓度的IBA对根诱导的影响,并对试管苗的移栽成活率进行了比较。结果表明:丛生芽诱导的最适培养基为MS+6-BA 3.0 mg/L+NAA 0.5 mg/L;试管苗在1/2MS+IBA 0.5 mg/L的生根培养基上生根效果最佳;在练苗3 d时,试管苗移栽的成活率最高,达95%以上。通过调节生长素与细胞分裂素的浓度比例可以有效的控制菊花丛生芽的分化、生长及生根。 相似文献
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以“圣香”苦瓜、“玉华”苦瓜的子叶为外植体进行离体培养植株再生研究.结果表明:2个苦瓜品种的子叶在不同的培养基上都较易形成愈伤组织,愈伤组织诱导率都达到80％以上.培养基MS+TDZ 0.05 mg/L+-NAA 0.02 mg/L适合“圣香”苦瓜子叶不定芽分化,分化率为68.4％;培养基MS+-TDZ 0.03 mg/L+-NAA 0.02 mg/L适合“玉华”苦瓜子叶不定芽分化,分化率为67.8％.“圣香”苦瓜在培养基上MS+ZT 0.3 mg/L+NAA 0.01 mg/L上丛生芽诱导效果好,增殖率达6.5;“玉华”苦瓜丛生芽诱导的最佳培养基为MS+TDZ 0.02 mg/L+NAA0.01 mg/L,增殖率达6.6.生根诱导以1/2MS+NAA 0.05 mg/L培养基诱导率最高,“圣香”苦瓜生根率为90.7％,“玉华”苦瓜生根率达91.9％. 相似文献
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激素对菊花愈伤组织诱导和丛生芽分化的影响 总被引：2，自引：1，他引：1
利用菊花的叶片为外植体,在附加不同激素浓度的MS培养基上诱导愈伤组织。通过对6-BA和NAA在菊花愈伤组织诱导和丛生芽作用的研究,筛选合适的激素配比。结果表明:愈伤组织诱导的最适培养基为MS+6-BA 2.0 mg/L+NAA 1.0 mg/L,丛生芽诱导的最适培养基为MS+6-BA 2.0~3.0 mg/L+NAA 0.1~0.5 mg/L,可获得较高的分化率。丛生芽继代培养基为MS+6-BA 2 mg/L+NAA 0.1 mg/L;试管苗在1/2MS+NAA 0.1 mg/L+20 g/L蔗糖的生根培养基上均可生根。 相似文献
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不同红掌品种在组培生产上的差异表现 总被引：1，自引：0，他引：1
以"亚利桑那"、"阿拉巴马"、"粉冠军"3个红掌品种根茎、叶柄、叶片为外植体,研究比较了3个品种的外植体诱导、继代增殖、生根培养的差异表现。结果表明:根茎诱导无菌芽成功率为86.9～90.0%,叶片诱导愈伤组织成功率为80%～84%,叶柄诱导愈伤组织成功率为64%～68%,根茎一般较难诱导出愈伤组织;不同品种的红掌增殖继代的增殖倍率不同,"粉冠军"最高为6.8,其次为"亚利桑那"5.8,"阿拉巴马"为3.8。红掌的生根较易,不同品种的红掌生根率均可达到95%以上。 相似文献
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以菊花不同外植体为试材,采用离体培养快速繁殖的方法,研究菊花不同外植体在不同的培养基中诱导不定芽再生的频率。结果表明:菊花不同基因型外植体在含适宜6-BA、NAA等激素的培养基中形成愈伤组织的难易程度不同,愈伤组织诱导形成不定芽的能力强弱也不一样;不同基因型菊花外植体的不定芽诱导率高低排序是花蕾花瓣茎段叶片;对菊花的花蕾、花瓣、茎段和叶片来说最适合的培养基配方是MS+6-BA 2.0mg/L+NAA 0.2mg/L,不同培养基诱导不定芽的能力为花蕾花瓣茎段叶片,其中,菊花花蕾和花瓣的再生能力较强。最合适的生根培养基是1/2MS+NAA 0.2mg/L。该研究成功的建立了菊花不同外植体离体培养再生途径,并获得了再生植株。 相似文献
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以翠菊‘花束绯红’无菌苗为材料,研究了植物生长调节剂浓度及组合、光照条件、不同附加物对叶片及其愈伤组织再生植株的影响。结果表明:(1)在光照培养条件下,诱导翠菊叶片不定芽形成的最适培养基为MS+6-BA3.0mg·L-1+IBA1.0mg·L-1,诱导率为56.7%,平均不定芽数为3.3;(2)黑暗培养不利于叶片不定芽的分化,但促进叶片愈伤组织的形成;(3)培养基中分别添加脯氨酸、水解酪蛋白和NH4NO3等均能明显促进愈伤组织不定芽的诱导,最适培养基为MS+6-BA1.0mg·L-1+IBA0.1mg·L-1+脯氨酸300mg·L-1,诱导率为82.8%,平均不定芽数为4.5;(4)不定芽移到幼苗生根培养基(1/2MS+IBA0.1mg·L-1)上,生根率为89.1%,移栽后成活率达89.3%。 相似文献
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60Co-γ辐射对切花菊试管苗的诱变效应 总被引：1，自引：0，他引：1
以‘神马’和‘长紫’两个切花菊品种的生根试管苗为试材,用60Co-γ射线进行辐射,设0（对照）、10、15和20 Gy等4个剂量处理,处理后以茎段和叶片为外植体进行离体培养,分析辐射对腋芽发生率、愈伤组织诱导率和分化率的影响,统计M1代田间主要性状及变异情况。结果表明：γ射线对试管苗茎段和叶片的愈伤组织诱导及分化有明显抑制作用,随着辐射剂量的增加抑制作用加强。不同品种、不同外植体对辐射的敏感程度都存在差异。茎段较叶片更适合做辐射后组培的外植体。‘长紫’M1代株高降低,花径减小;而‘神马’在株高和花径出现略微增加的趋势。茎段和叶片的再生植株田间主要性状的变异程度大于腋芽的再生植株。‘长紫’在花色和瓣形上的变异率高于‘神马’。 相似文献
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T. R. Marks Sally E. Simpson 《The Journal of Horticultural Science and Biotechnology》2013,88(3):543-537
SummaryAxillary shoot cultures of both Acer saccharinum L. ‘Pyramidale’ and A. platanoides L. ‘Crimson King’ displayed strong apical dominance and prolific basal callus in vitro, which was not conducive to rapid multiplication and rooting. Basal callus was reduced in ‘Pyramidale’ by replacing 6-benzylaminopurine (BAP-5μM) with zeatin (5μM), but this also reduced axillary shoot growth. The addition of 2,3,5-triiodobenzoic acid (1-20 μM) altered callus development and promoted a concentration-dependent increase in axillary shoot growth. Supplementing medium with thidiazuron (0.005 and 0.05 μM) in addition to BAP (1 μM) enhanced shoot growth, especially with nodal shoot sections, and increased subsequent rooting. Although thidiazuron also increased basal callus, this correlated with better shoot growth in ‘Crimson King’. Selection of apical buds from ‘Crimsom King’ stockplants was essential for the establishment of sustainable cultures; axillary bud-derived expiants quickly died. Once shoots of ‘Pyramidale’ and ‘Crimson King’ had elongated, they could be readily rooted in vitro, and plantlets were successfully weaned under high humidity ‘dry fog’. 相似文献
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以野生水芹茎尖、腋芽为外植体,研究了影响愈伤组织诱导和分化的激素浓度配比.结果表明,最佳外植体是茎尖;最佳愈伤组织和不定芽诱导培养基为MS 6-BA 4 mg/L,最佳继代增殖培养基为MS 6-BA 2 mg/L,最佳生根培养基为MS IBA 1 mg/L.并提出试管苗炼苗与移栽的最佳试验条件. 相似文献
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天女木兰嫩茎愈伤组织诱导及再生体系建立研究 总被引：1，自引：0，他引：1
为了保护濒危植物天女木兰,采用植物组织培养方法,以嫩茎为材料,进行愈伤组织诱导与分化、不定芽生根与试管苗生根继代增殖的培养,以及试管苗移栽与定植的研究,建立起天女木兰再生体系技术.结果表明:MS+ZT 0.4 mg/L+2,4-D 2.5 mg/L是嫩茎愈伤组织诱导培养和继代增殖培养的理想培养基;MS+GA30.5 mg/L+BA 0.6 mg/L是愈伤组织分化培养的理想培养基;把不定芽用10 mg/L的IAA溶液处理24 h后,接种到1/3 MS+IBA 0.6 mg/L+0.8 DA-60.5 mg/L+NAA 0.5 mg/L培养基上的生根培养方法是不定芽生根培养和试管苗生根继代增殖培养的理想方法;在温室中试管苗易移栽成活,定植的试管苗保持了野生天女木兰的所有植物学性状. 相似文献
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白花紫露草的组织培养与植株再生体系的建立 总被引：3，自引：1，他引：2
以白花紫露草嫩茎为材料,进行了愈伤组织诱导、分化、试管苗生根、试管苗移栽所需条件的研究。结果表明：1/2MS＋BA 0.5 mg/L＋NAA 0.2 mg/L是诱导愈伤组织的理想培养基;MS＋BA 1.0 mg/L＋2,4-D 0.5 mg/L是诱导愈伤组织形成,同时具有分化能力愈伤组织的理想培养基;1/2MS＋BA 0.2 mg/L＋NAA 0.1 mg/L是诱导愈伤组织和不定芽分化的理想培养基;1/2MS＋IAA 0.3 mg/L是生根培养的理想培养基;以炉灰渣为试管苗的移栽扦插基质,移栽成活率为98%,扦插成活率为91%。 相似文献
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SummaryThe consequences of using ex vitro, single-node explants from different topophysical positions in chrysanthemum (Chrysanthemum grandiflorum /Ramat./ Kitam) were determined. In particular, how explant topophysis affected the rate of propagation, which is important for the successful micropropagation of chrysanthemum. Uniform shoots of five cultivars of chrysanthemum, cultured in vitro, were each divided into three equal zones: distal, central, and proximal. Two single-node explants were isolated from each zone and cultured on MS medium without any added growth regulators. After 10 weeks of culture, 50% of the shoots that had developed from axillary buds on each single-node explant were excised and measurements were taken in order to compare those shoots that had developed from explants from the different topophysical zones. The remaining shoots were sub-cultured on rooting medium. After 4 weeks, the numbers of roots per plantlet, and the total fresh weight (FW) of roots were recorded. The cultivars fell into two groups. ‘Lady Amber’, ‘Lady Orange’, and ‘Lady Vitroflora’ explants were topophysis-dependent, while ‘Lady Bronze’ and ‘Lady Rosy’ explants were topophysis-independent. For the three topophysis-dependent cultivars, the propagation rate, growth rate, shoot length, internode length, single leaf weight, and total plantlet FW values were highest for those shoots derived from the central and proximal zones. Topophysis failed to affect the number of leaves per shoot or the number of days between the appearance of two successive leaves. The effects of topophysis on the number of roots per plantlet and on root FW were inconsistent. The unequal growth of chrysanthemum plantlets during in vitro micropropagation can be an effect of topophysis, and this phenomenon is cultivar-specific in chrysanthemum. 相似文献
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Sequential subculturing leads to a gradual physiological change in cells that may be termed ‘rejuvenation’. The effect of repetitive subculturing on callus induction and shoot regeneration from leaf explants of Punica granatum L. ‘Kandhari Kabuli’ were investigated. Surface-sterilised leaves were cultured on 1.0× Murashige and Skoog (MS) medium supplemented with 4.0 mg l–1 α-naphthaleneacetic acid (NAA) and 2.0 mg l–1 6-benzyladenine (BA) for callus induction. Shoots were regenerated from callus on 1.0× MS medium supplemented with 1.5 mg l–1 BA, 0.5 mg l–1 kinetin, and 0.25 mg l–1 NAA. Subculturing of callus onto fresh medium maintained the rate of shoot formation and substantially increased the production of shoot buds up to the second subculture. Following further subculture passages, a lower shoot regeneration potential from callus was observed. A maximum shoot bud induction from callus of 63.9% was observed at the second subculture passage. The rate of multiplication of in vitro shoots increased until the fourth subculture, then became constant. Similarly, in vitro rooting of micro-shoots increased up to the third subculture, followed by a decline during further subculturing. 相似文献