共查询到19条相似文献，搜索用时 218 毫秒
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【目的】将苹果Md DRB1基因转入番茄中,探究苹果MdDRB1基因在番茄中的异位表达与功能。【方法】采用农杆菌介导法,将苹果基因Md DRB1转入番茄植物体内,用PCR检测到该基因已整合到番茄总DNA中。对获得的5个转基因番茄株系进行半定量RT-PCR分析,挑选MdDRB1基因表达水平由低到高的3个株系,用实时荧光定量PCR检测与生长素有关的mi RNAs及相应靶基因的相对表达量。并对非转基因和转基因番茄进行3μmol·L-1IAA和5μmol·L-1IAA处理,对植株进行向光性检测。【结果】转基因株系中miR164、miR160、mi R393表达量下调,mi R167表达量上调,同时,它们分别对应的番茄中的靶基因SlNAC1、SlARF10和SlARF16、SlTIR1的相对表达量上调,SlARF8相对表达量下调。IAA处理后,转基因株系幼苗的侧根数目比未转基因植株增多,同时,转基因株系幼苗的向光性也增强。【结论】苹果Md DRB1基因在番茄中的异位表达,通过抑制生长素响应相关的mi R164、miR160、miR393和增加miR167的积累水平,部分解除其对下游靶基因SlNAC1、SlARF10和Sl ARF16、SlTIR1以及增加Sl ARF8的转录后基因沉默作用,进而提高了转基因番茄对生长素响应的敏感性。 相似文献
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《中国瓜菜》2016,(8)
为探讨利用外源物质调控番茄果实中番茄红素积累的机制,以高代自交系番茄为材料,进行K~+、Mn~(2+)处理后对其果实不同转色期的番茄红素及其相关酶的基因表达量进行动态监测。结果表明:K~+处理下的番茄红素含量在果实进入半熟期后积累迅速,至完熟期明显高于对照,K~+处理下各种酶基因表达量的变化与番茄红素含量积累规律一致,在果实发育坚熟期至完熟期PSY1(八氢番茄红素合成酶1)、PSY2(八氢番茄红素合成酶2)、PDS(八氢番茄红素脱饱和酶)、ZDS(ζ-胡萝卜素脱饱和酶)基因的表达量增加,该阶段的番茄红素含量也迅速积累;Mn~(2+)处理下果实番茄红素含量一直低于对照,其番茄红素积累迅速期在果实坚熟期至完熟期,Mn~(2+)处理下果实转色5个时期的PDS、ZDS基因表达量均明显低于对照。Mn~(2+)抑制了PDS、ZDS的表达从而降低了番茄红素含量的积累;然而,Mn~(2+)处理下番茄果实坚熟期至完熟期PSY1、PSY2基因表达量迅速升高,促使番茄红素含量在该阶段迅速积累。本研究说明番茄果实发育坚熟期至完熟期可通过K~+调控而使该阶段的番茄红素含量迅速积累,可通过使用外源物质来调控番茄果实中番茄红素的含量。 相似文献
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春播番茄会产生一种畸形果。它的产生与番茄花芽的发育外界条件有着密切关系。一般来说,番茄果实的形成,首先从花芽分化到果实成熟,须经过花原基的分化,各种花器官的形成,然后才能现蕾、开花、授粉、受精及子房的发育,最后达到果实成熟。首先从花芽分化到开花,要经过一系列的过程,即萼片、花瓣、雄蕊等 相似文献
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利用不同浓度的茉莉酸甲酯（MeJA）对葡萄组培植株进行处理,进而分析其生理及基因表达变化,结果表明MeJA能抑制植株不定根的分化和伸长,诱导根、茎、叶组织中的脱落酸（ABA）含量升高,但降低赤霉素（GA）、生长素（IAA）和细胞分裂素（ZR）的含量,且影响叶片中叶绿素代谢导致褪绿黄化。MeJA能诱导植物体内茉莉酸合成代谢基因表达进而提高内源茉莉酸的含量,诱导ABA合成代谢和信号路径中的基因表达,但是会抑制生长素、赤霉素、细胞分裂素、油菜素内酯合成代谢基因的表达,抑制了与根发育和延伸相关基因VvXET、VvEXPB2、VvEXPA7和VvWUS的表达,进而抑制不定根的发生,导致植物发育缓慢。因此,MeJA通过影响其他激素的合成代谢来调控它们在植物中的含量,导致内源激素不平衡影响植株发育,并调控植株根的发育状态,在不定根发育方面起负向调控作用。 相似文献
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不同生育期弱光胁迫对番茄生长及产量的影响 总被引：1，自引：0，他引：1
于番茄的开花期、果实膨大期和果实绿熟期进行持续5周的50%自然光强的弱光处理。试验结果表明:果实绿熟期的弱光胁迫对植株干物重、各器官的物质分配率和果重影响程度最小,果实膨大期的弱光胁迫使番茄果重显著降低,而开花期弱光胁迫使坐果率和单果重显著下降,导致产量显著降低。因此,番茄对弱光的敏感程度依次为开花期>果实膨大期>果实绿熟期。 相似文献
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以番茄（Lycopersicum esculentum）LA1996（Aft基因型,紫色花青素积累品系）cDNA为模板,对LemiR828前体基因和预测到的靶基因LeTAS4（Trans-Acting SiRNA Gene 4）进行克隆,采用RT-PCR技术检测其在番茄果实发育中的差异表达,并用RLM 5′RACE技术验证LemiR828对预测靶基因LeTAS4 mRNA的剪切作用及靶作用位点。结果表明,LemiR828的前体序列含有完整的茎环结构;在果实发育成熟的过程中,LemiR828及LeTAS4在番茄中的表达量存在差异。在绿色果阶段的表达量较低且LemiR828对LeTAS4的负调控关系不明显;而在成熟果阶段,LemiR828及LeTAS4的表达量均升高,且存在明显的负调控关系。LemiR828能够靶作用于LeTAS4的转录本上调控其降解,其剪切位点为LemiR828序列5′端的第10位碱基。因此,在LA1996番茄果实发育过程中,LemiR828和LeTAS4参与果实发育的调控,并且LemiR828负调控其靶基因LeTAS4的表达。 相似文献
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将从欧李果实中分离的ChPSY cDNA经过序列分析、酶切之后,连接到植物表达载体PMV,构建了植物重组载体pBI-ChPSY,并通过根瘤农杆菌EHA105介导转化番茄,获得了12株抗性植株。PCR检测和Southern杂交结果显示,12株抗性植株均为阳性,说明外源基因ChPSY整合到转化植株的基因组中。RT-PCR分析表明,ChPSY基因在转化植株果实中得到表达。HPLC分析表明,转ChPSY基因番茄果实中总类胡萝卜素含量增加了1.62 ~ 3.04倍,八氢番茄红素、番茄红素、β–胡萝卜素和α–胡萝卜素含量分别增加了4.87、2.10、2.59和3.25倍。转化植株中内源类胡萝卜素合成基因的表达也受到广泛的影响。ChPSY基因超量表达有效促进了番茄果实中类胡萝卜素的合成和积累。 相似文献
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为了深入研究SlIAA14基因的表达模式及其调控番茄根系发育的分子机理,利用染色体步移技术从番茄品种‘Ailsa Craig’中克隆到该基因上游2 197 bp的启动子片段。生物信息学分析表明,该启动子片段中含有多个对脱落酸、赤霉素、细胞分裂素等激素响应的顺式作用元件,以及对干旱和伤害等逆境胁迫响应的顺式作用元件。利用农杆菌介导的遗传转化方法获得SlIAA14：：GUS + YFP融合基因的转基因番茄植株,转基因植株根系YFP和GUS检测结果显示,该启动子片段在番茄根尖和侧根原基有较高的表达活性,表明SlIAA14基因可能调控番茄根系的伸长和侧根发育的起始。 相似文献
9.
番茄花柄离区发育基因JOINTLESS及互作蛋白基因的功能研究 总被引：1，自引：0，他引：1
番茄离区发育控制基因JOINTLESS（J）是一个MADS-box家族基因。MADS-box基因是植物花发育过程中一类重要的转录因子,MADS-box蛋白调节高等植物发育过程中往往形成复合体控制基因的转录。为了研究J及其互作蛋白在番茄发育过程中的重要作用,选择7个与JOINTLESS蛋白互作的MADS-box基因进行RNAi功能研究。研究结果表明TAG1基因不仅严重影响番茄雄蕊和雌蕊的形态,还影响果实的育性。同时对J进行了过量表达分析,研究结果表明,J过量表达不但显著增加了番茄花序的分支和花的数量,还改变了果实形状。 相似文献
10.
一氧化氮对番茄果实采后成熟和Le-ETR4基因表达的影响 总被引：2，自引：0，他引：2
以破色期的‘卡罗’番茄果实为试材,研究了NO供体硝普钠（SNP）50 μmol · L-1处理30 min对番茄果实采后成熟的作用,并采用Northern杂交技术检测NO对番茄乙烯受体基因Le-ETR4表达的影响。结果表明,50 μmol · L-1 SNP处理降低了番茄果实的乙烯释放速率,抑制了ACC氧化酶（ACC oxidase,ACO）、纤维素酶（Cellulase）和多聚半乳糖醛酸酶（Polygalacturonase,PG）的活性,延缓了果实的色泽变化和软化,同时显著抑制了番茄乙烯受体基因Le-ETR4的表达。以上结果说明NO主要通过抑制番茄乙烯的生物合成和乙烯受体基因表达来控制成熟相关酶的活性,NO可以在生理水平和乙烯受体水平调控番茄果实的成熟。 相似文献
11.
V. Kagan-Zur D. Livne Y. Mizrahi 《The Journal of Horticultural Science and Biotechnology》2013,88(6):817-825
Selfed and auxin-treated fruits of tomato (Lycopersicon esculentum Mill.) were obtained from tetraploid and diploid plants and compared to determine why selfed tetraploid fruits are smaller. Application of auxin to ovaries produced tetraploid fruits as large as normally set (selfed) diploid fruits but led to reduced fruit size in diploid fruits, reflecting a further potential for tetraploid fruit development. The selfed tetraploid fruits were found to be smaller because they had fewer cells per fruit. Cell size (diameter) at the end of fruit development was similar for all fruit types. DNA per cell did not closely match cell size during development, especially during the cell division stage. Protein per cell also failed to follow cell size. Protein per DNA showed one peak at around day 10 of fruit development and possibly a second one at day 30. 相似文献
12.
A. A. Ramin 《The Journal of Horticultural Science and Biotechnology》2013,88(5):706-710
SummaryTomato (Lycopersicon esculentum L. Mill) cultivars Early Urbana and Red Clode were grown in the field at adverse temperatures (stress regimes) until they set fruit in two growing seasons (Autumn and Spring) during 1999 and 2000 in Ahwaz (Southern Iran). To test the effectiveness of auxins in enhancing fruit set, racemes of tomato were sprayed during early fruit set with 25, 50 and 100 mg l–1 4-chlorophenoxy acetic acid (4-CPA) and indole-3-butyric acid (IBA) followed by two additional applications at three intervals. The control plants were treated with deionized water. At harvest, racemes from autumn and spring plantings that were treated with 4-CPA had more large fruits than the control racemes. In both growing seasons, neither cultivar showed a significant difference in fruit set in plants treated with IBA, compared with controls. The ratio of fruit/flower in all racemes treated with 4-CPA increased significantly compared with the other treatments. However, auxin treatments did not significantly affect the number of flowers per raceme. Plants treated with 4-CPA produced the highest yield of marketable fruit. The greatest increase in total yield and parthenocarpic fruit occurred with 4-CPA treatments. Also, there was little difference between concentrations of 4-CPA on fruit set in both cultivars. It may be concluded that the response of fruit setting in tomato to either low or high temperatures regimes was auxin dependent and that 4-CPA was more effective than IBA. 相似文献
13.
《Scientia Horticulturae》2001,89(4):309-316
The effect of fruit load and auxin application on the flower morphology and flower number of two aubergine cultivars was investigated under greenhouse conditions. The flowers of plants that did not set fruit showed only minor fluctuations in flower and pistil mass, and in style and anther length. However, when plants were allowed to set fruit by pollination, flower and pistil mass, and style (but not anther) length were significantly reduced during the stage of fruit growth. Style length was also reduced in flowers formed after fruit had been set parthenocarpically by the application of auxin (IAA), but there was no significant effect of IAA on style length in the absence of fruit development. The number of flowers was reduced in the presence of IAA (with or without fruit set), or following fruit set by pollination. It is suggested that fruit load and development affect flower mass and style length of aubergine whereas auxin influences the number of flowers. 相似文献
14.
The effects of root colonization by the arbuscular mycorrhizal (AM) fungus Glomus intraradices Schenck and Smith on growth, flower and fruit production, and fruit quality were studied in field-grown tomato plants exposed to varying intensities of drought stress. Inoculated (M+) and non-inoculated (M−) tomato seedlings were exposed to varying intensities of drought stress by adjusting irrigation intervals. Mycorrhizal plants had significantly higher uptake of N and P in both roots and shoots regardless of intensities of drought stress. AM inoculation also significantly increased shoot dry matter and the number of flowers and fruits. The fruit yields of M+ plants under severe, moderate, mild drought-stressed conditions were higher than M− plants by 24.7%, 23.1%, 16.2% and 12.3%, respectively. Furthermore, M+ plants produced tomato fruits that contain significantly higher quantities of ascorbic acid and total soluble solids (TSS) than M− plants. Mycorrhizal effects increased with increasing intensity of drought. The overall results suggest that mycorrhizal colonization affects host plant nutritional status, water stratus and growth under field conditions and thereby alters reproductive behaviour, fruit production and quality of fruits under both well-watered and drought-stressed conditions. 相似文献
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16.
森林草莓醇酰基转移酶基因FvAATW2功能研究 总被引：1，自引：0，他引：1
在从森林草莓(Fragaria vesca L.)成熟果实中克隆到醇酰基转移酶基因(FvAATW2)的基础上,构建由CaMV35S启动子驱动的植物正义表达载体pBI121-FvAATW2,在烟草(Nicotiana tabacum L.)和草莓(Fragaria×ananassa Duch.)‘Camarosa’品种中过量表达FvAATW2,以研究其功能。采用农杆菌介导的叶盘法转化烟草和草莓;利用PCR和Southern blot筛选出转基因株系;通过实时定量PCR和测定AAT酶活性来检测转基因植株中外源基因的表达;利用SPME/GC–MS方法检测烟草叶片和草莓果实中的挥发性成分。对转基因烟草外源基因表达和早期叶片挥发性成分的检测结果表明,构建的FvAATW2表达载体功能正常,转基因株系能够在生长早期合成酯类物质;通过检测转基因草莓成熟果实的酯类成分发现,与野生型对照相比,转基因草莓果实中酯类占挥发物的总比例以及乙酸辛酯、己酸乙酯、己酸辛酯、辛酸乙酯等挥发酯的比例显著提高,而丁酸甲酯的含量显著降低,辛酸乙酯的含量显著增加,说明外源FvAATW2在草莓中能够正常表达并影响果实酯类合成,从而通过改变挥发性酯类构成使果实香气变浓。 相似文献
17.
以4个不同番茄砧木品种与夏日阳光樱桃番茄进行嫁接,以夏日阳光自根苗为对照(CK),研究了不同砧木嫁接对樱桃番茄的生长发育、抗病性、抗旱性、品质及产量的影响。试验结果表明,4种砧木嫁接均能显著降低青枯病发病率,其生长势、产量均显著优于自根苗,且品质和口感与自根苗无明显差异,其中,以哥俩好的成活率最高,为95.8%,第一雌花节位最低,采收期最长,青枯病发生率最低,生长势最强,坐果率最高,单果质量最大,糖酸比最大,达7.301,产量最高,达4 858.0 kg/667 m~2,综合表现优异,适宜作为樱桃番茄夏日阳光的嫁接砧木进行推广。 相似文献
18.
Sabry M. Youssef Silvia Jiménez-Bermúdez M. Luz Bellido Carmen Martín-Pizarro Marta Barceló Samia Abdallah Abdal-Aziz José L. Caballero José M. López-Aranda Fernando Pliego-Alfaro Juan Muñoz Miguel A. Quesada José A. Mercado 《Scientia Horticulturae》2009
Two transgenic strawberry lines (Pel 1 and Pel 3) containing the open reading frame of a fruit specific strawberry pectate lyase gene (FaplC) under the control of the CaMV35S promoter have been obtained to evaluate the role of this gene on fruit softening. Ripen fruits from both lines showed a significant down-regulation of FaplC, being the percentage of silencing of 84 and 71% on Pel 1 and Pel 3, respectively. The agronomic behaviour of transgenic plants was evaluated during two consecutive years. Fruit set increased in the two transgenic lines when compared with control plants, although Pel 1 showed a significant reduction on fruit weight. Firmness of full ripen fruits from Pel lines was significantly higher than control fruits, while color and soluble solids were not affected. The increase of firmness in Pel lines was maintained when ripe fruits were stored for 3 days at 25 °C. Histological analysis of ripe fruits showed lower intercellular spaces and a higher degree of cell to cell contact area in transgenic fruits when compared with controls. Altogether, these results suggest a direct relationship between pectate lyase gene expression and strawberry fruit softening. 相似文献
19.
以高丛越橘(Vaccinium corymbosum)大果型品种‘奥尼尔’和小果型品种‘蓝雨’为试材,测定花芽膨大与果实发育期间生长曲线及成熟果实的部分生理指标;开展包含PLAC8保守结构域的FWL(fruit weight like)基因全基因组鉴定与序列分析,并应用实时荧光定量PCR法检测VcFWL/PLAC8基因亚家族成员在花芽膨大与果实发育进程中的相对表达水平。结果表明:‘奥尼尔’果实单果质量与横径自S2期起均显著高于‘蓝雨’,其成熟果实中心室数、单果种子数及种子质量也显著高于‘蓝雨’果实。越橘VcFWL/PLAC8家族包含11个成员,分布于10条染色体上,结构分析显示这些基因均包含2~3个外显子。大部分VcFWL/PLAC8基因包含高度保守的结构域,聚类分析可将其分为3个亚家族,其中B和C亚家族成员间高度保守。qPCR分析表明A与C亚家族基因的表达模式和越橘花芽与果实发育进程中细胞增殖趋势一致,B亚家族基因的表达丰度较低,推测VcFWL/PLAC8基因可能通过不同的机制和途径参与调控果实的生长发育。 相似文献