共查询到20条相似文献，搜索用时 609 毫秒
1.
2.
本文通过给山羊静注大肠杆菌的内毒素（ＥＴ）复制ＥＴ休克模型,探讨ＥＴ休克时山羊肾脏的病理损伤,并了山莨菪碱（６５４－２）对其影响。结果表明,临床病理学上,出现混合型酸中毒指标的变化并有ＤＩＣ（弥漫性血管内凝血）的发生。病理组织学特点为肾小球毛细血管扩张,步球内有中性粒细胞和血小板聚集,内皮细胞和系膜细胞肿胀或增生、肾小管上皮细胞呈退行性变化,且肾脏有明显的超微结构变化。６５４－２能有效地缓解或减轻 相似文献
3.
山莨菪碱（654—2）对内毒素诱导山羊红细胞损伤过程中膜脂流动性？… 总被引：1，自引：1，他引：0
用１,６－二苯基－１,３,５－已三烯（１．６－ｄｉｐｈｅｎｙｌ－１,３,５－ｈｅｘａｔｒｉｅｎｅ,ＤＰＨ）分子荧光偏振技术,研究了内毒素（ＥＴ）诱导的山羊红细胞膜（ＥＣＭ）损伤过程中膜脂的流动性（Ｐ）、膜脂区微粘度（η）、脂双层分子列有序性系数（Ａ）的变化及山莨菪碱（６５４－２）对其的影响。结果表明,ＥＴ处理组（Ⅱ组（）ＥＣＭ的Ｐ、η和Ａ参数在处理后３、５、７、９、１２小时均显著高于Ⅰ组Ⅲ组（Ｐ〉 相似文献
4.
内毒素诱导山羊肝线粒体膜流动性的变化及654—2效应 总被引：4，自引：0，他引：4
用１,６－二苯基－１,３,５－己三烯（１,－６－ｄｉｐｈｅｎｙｌ－１,３,５－ｈｅｘａｔｒｉｅｎｅ,ＤＰＨ,Ｓｉｇｍａ Ｃｈｅｍｉｃａｌ Ｃｏ。）分子荧光振技术研究了内毒素（ＥＴ）诱导的山羊肝线粒体膜（ＭｉＭ）损伤过程中膜脂的流动性,膜脂区微粘度及脂双层分子排列有序性系数的变化及６５４－２的影响。 相似文献
5.
采用 １,６二苯基１,３,５己三烯（１,６ｄｉｐｈｅｎｙｌ１,３,５ｈｅｘａｔｒｉｅｎｅ, Ｐ Ｈ, Ｓｉｇｍ ａ Ｃｈｅｍ ｉｃａｌ Ｃｏ）分子荧光偏振法研究了内毒素诱导山羊红细胞膜的流动性（ Ｐ）,膜脂区微粘度（η）及脂双层分子排列有序性系数（ Ａ）的变化及６５４２ 的影响。结果表明, Ｅ Ｔ 处理组（Ⅱ组） Ｅ Ｃ Ｍ 的 Ｐ、和 Ａ参数除在１ｈ 高于对照组（Ⅰ组）和６５４ Ｚ２处理（Ⅲ组）且差异不显著（ Ｐ＞ ０．０５）外,在 ３,５,７,９,１２ｈ 均高于Ⅰ组和Ⅲ组（ Ｐ＜０．０５）, Ｐ＜ ０．０１）;Ⅲ组 Ｐ、和 Ａ 参数在１ ｈ低于Ⅰ组且差异不显著（ Ｐ＞ ０．０５）,在３ ｈ显著低于Ⅰ组（ Ｐ＜ ０．０１）,在５ 和７ ｈ 高于Ⅰ组（ Ｐ＜ ０．０５, Ｐ＜ ０．０１）,在９ ｈ,１２ ｈ 虽高于Ⅰ组但无显著差异（ Ｐ＞ ０．０５）。结果显示, Ｅ Ｔ 可使 Ｅ Ｃ Ｍ 荧光偏振度（ Ｐ）、微粘度（η）和脂双层分子排列有序性（ Ａ）增加。膜流动性降低,而６５４２ 能有效地改善 Ｅ Ｔ诱导的这些损伤变化,支持了６５４２ 具有维护膜结构和功能的生理作用的理论。 相似文献
6.
内毒素对山羊红细胞膜ATPase活性的影响及654—2的保护效应 总被引：5，自引：0，他引：5
通过静脉注射的方法研究了大肠杆菌仙毒素对山羊红细胞膜损伤的膜泵分子活性的变化及６５４－２（山莨菪碱）的保护效应。结果表明,ＥＴ处理组在１ｈ,３ｈ红细胞膜Ｎａ＾＋－Ｋ＾＋ＡＴＰａｓｅ（腺苷三磷酸酶,简称ＡＴＰ酶）,Ｃａ＾２＋,Ｍｇ＾２＋－ＡＴＰａｓｅ活性均高于对照组,５ｈ有下降趋势并一直持续到９ｈ。 相似文献
7.
狂犬病病毒糖蛋白基因在原核细胞中的表达 总被引：2，自引：0，他引：2
将狂犬病病毒糖蛋白（ＲＶｇｐ）基因ＢｇｌⅡ片段（１６７５ｂｐ）分别正向插入到原核高效表达载体ｐＥＴ－１７ｂ和ｐＥＴ－１７ｂ２（用ＳａｃⅠ－ＮｄｅⅠ缺失掉ｐＥＴ－１７ｂ６０ｂｐ含起始密码子ＡＴＧ小片段）的ＢａｍＨⅠ切点，构建重组质粒ｐＥＴ－１７ｂＲＶｇｐ和ｐＥＴ－１７ｂ２ＲＶｇｐ。将其分别转化表达受体菌Ｅ．ｃｏｌｉＢＬ２１（ＤＥ３）和Ｅ，ｃｏｌｉＢＬ２１（ＤＥ３）ｐｌｙｓＳ．ＩＰＴＧ诱导表达，菌体经超声波裂解处理后ＳＤＳ－ｐＡＧＥ，染色，在分子量约６００００处可见重组质粒表达的较宽的蛋白带，以抗ＲＶｇｐＭｃＡｂ进行Ｗｅｓｔｅｒｎ－ｂｌｏｔ检测，表明该表达蛋白为ＲＶｇｐ。通过扫描显示，表达的ＲＶｇｐ占菌体总蛋白的１０％～１４％，其中ｐＥＴ－１７ｂ２ＲＶｇｐ在Ｅ。ｃｏｌｉＢＬ２１（ＤＥ３）中的表达量最高。 相似文献
8.
HIV—2env基因在大肠杆菌和重组痘苗病毒中的表达 总被引：1，自引：0，他引：1
将编码人Ⅱ型免疫缺陷病毒（ＨＩＶ－２）ｅｎｖｇｐ１２０（Ｅ１）和ｇｐ３６（Ｅ２）分别克隆到原核高效表达载体ｐＥＴ１７ｂ、ｐＢＶ２２０和真核表达载体ｐ１０、ｐ１６中,构建成８个重组质粒。经ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｒｎｂｌｏｔ分析证明,在原核表达系统成功地表达了ＨＩＶ－２ｅｎｖ融合蛋白,表达的蛋白分子量分别为３５０００、７００００和５００００,而原核表达质粒ｐＥＴＥ１在ＳＤＳ－ＰＡＧＥ凝胶的３５０００处可见明显的额外蛋白带。经薄层扫描测定,原核系统表达的Ｅｎｖ蛋白（ｇｐ１２０）相对含量为５．８％。在真核细胞中表达的Ｅｎｖ蛋白经Ｗｅｓｔｅｒｎｂｌｏｔ检测分析,分子量分别为６００００、５７０００和４２０００。上述表达的Ｅｎｖ蛋白均能与ＨＩＶ－２阳性血清发生特异性反应。重组痘苗病毒可诱导小鼠产生抗ＨＩＶ－２Ｅｎｖ抗体 相似文献
9.
10.
马新武 《国外兽医学(畜禽疾病)》1996,17(4):1-4
内皮素是哺乳动物体内新发现的一类具有广泛生物学活性的短链多肽，是人及动物体内许多器官组织中都有ＥＴ及其受体分布。同一体内至少有ＥＴ－１、ＥＴ－２、ＥＴ－３三种ＥＴ异构体形式和ＥＴＡ、ＥＴＢ两种ＥＴ受体亚型。 相似文献
11.
为探讨内毒素（ET）对大鼠肝脏c—myc基因的表达影响及阳离子（cA）对该过程的保护效应,本试验将72只清洁级SD大鼠随机分为对照组、内毒素组和阳离子A保护组,3组经相应处理后分别在3、4、8、12h采集肝脏作为样本。采用实时荧光定量RT—PCR检测法和免疫组化法分析,结果显示：在mRNA水平和蛋白水平上,ET可上调C—myc基因的表达,从而促进肝细胞凋i-,最终导致肝细胞损伤,CA则能明显下调c—myc基因的表达,从而抑制肝细胞凋亡,对ET诱导的肝损伤具有明显的保护效应。 相似文献
12.
Effects of endotoxin on carbohydrate metabolism were studied in A/HeJ (endotoxin-sensitive) and C3H/HeJ (endotoxin-resistant) inbred mice. A/HeJ mice developed hypoglycemia within two hours after endotoxin injection, yet liver glycogen content did not differ from controls. Similarly treated C3H/HeJ mice did not develop significant hypoglycemia. Administration of glucagon to endotoxin-treated A/HeJ mice failed to elevate their blood glucose concentrations, while endotoxin-treated mice of the same strain did respond to dibutyryl cyclic AMP with a significant elevation of blood glucose. C3H/HeJ mice on the other hand responded to glucagon and dibutyryl cyclic AMP with elevated blood glucose. Endotoxin-treated C3H/HeJ but not A/HeJ mice were able to carry out gluconeogenesis induced by prednisolone, while both inbred strains showed active glycogenesis after administration of an exogenous glucose load. Administration of glucagon resulted in diminished liver glycogen concentrations in A/HeJ endotoxin-treated mice suggesting no impairment of glycogenolysis. The inability of endotoxin-treated A/HeJ mice to respond to glucagon could be due to impairment of gluconeogenesis. Although endotoxin interfered with the capacity of both inbred strains to respond to glucagon administration with elevation of liver cyclic AMP, the effect was significantly more severe in A/HeJ mice. The susceptibility of A/HeJ mice to the lethal effect of endotoxin may be related to the apparent sensitivity of carbohydrate metabolic pathways to disturbance by endotoxin. 相似文献
13.
O Ladefoged 《Acta veterinaria Scandinavica》1978,19(4):479-486
The action of bacterial endotoxin on the pharmacokinetics of warfarin was investigated in rabbits injected with bacterial endotoxin from E. coli. High doses of endotoxin were able to change the kinetics of warfarin in rabbits by changing its volume of distribution. It is not possible to conclude whether the changes in elimination rate of warfarin in rabbits were mediated through changes in liver blood flow or through impairment of the metabolic capacity of the liver cells. 相似文献
14.
犬冠状病毒在犬体内分布的初步研究 总被引：1，自引：0，他引：1
对电镜检查粪便中发现有犬冠状病毒的 4只犬 ,进行了血常规、尿常规、肝肾功能和心电图检查 ,均未发现明显异常。扑杀后经电镜负染检查 ,除肠内容物外 ,在其肝、心、脾、肾和肺等实质脏器中均发现冠状病毒粒子 相似文献
15.
Kokosharov T 《The Onderstepoort journal of veterinary research》2000,67(4):297-300
Bacterial colonization and endotoxin production were investigated before and after experimental Salmonella gallinarum infection in 8-week-old female broiler chickens. These parameters were assayed by means of colony forming units test (CFU) and the Limulus Amebocyte Lysate test (LAL), respectively. Birds were infected per os with 1,5 x 10(9) CFU/ml of wild strain of S. gallinarum isolated from a dead hen. Approximately 1,5 x 10(2); 1,3 x 10(2) and 1,2 x 10(2) CFU of S. gallinarum were recorded from 1 g of liver, 1 g of spleen and 1 ml of blood from the chickens on day 1 post infection. By day 4 corresponding data were 3,7 x 10(4); 4,8 x 10(3) and 1,1 x 10(3) respectively and on day 7 10(5) CFU were present in all three specimen types. The liver and spleen of dead birds were contaminated with more than 10(7) CFU per g. The endotoxin from S. gallinarum was found to have an activity of 1,5; 12,0 and 15,0 endotoxin units (EU)/ml on day 1, 4 and 7 after infection, respectively. No endotoxin activity was established in the blood of the control group (before infection) by the LAL test. This is the first time the connection between the amount of live S. gallinarum in the blood, liver and the circulating level of endotoxin in the blood during the infectious stage of experimental acute fowl typhoid, has been demonstrated. 相似文献
16.
铅镉联合急性中毒对大鼠脏器病理组织学和超微结构的影响 总被引：1，自引：0，他引：1
按照等毒性配比法混合硝酸铅与氯化镉溶液进行急性毒性试验,采用Bliss法和Keplinger标准进行毒性评价,并应用光镜和电镜观察大鼠染毒后各脏器的显微与超微结构变化。探讨硝酸铅（Pb（NO3）2）与氯化镉（CdCl2·2．5H2O）溶液联合经口灌胃对SD大鼠的急性毒性效应。结果显示,铅镉联合作用下大鼠的绝对致死量（LD100）为5062．00mg／kg,最大耐受浓度（MTD）为1436．00mg／kg,半数致死量（LD50）为2696．54mg／kg,95％可信区间是2162．00～3362．89mg／kg。铅镉联合急性中毒的靶器官为肝脏、肾脏和脾脏,光镜下,主要表现为血管发生不同程度的充血和溶血、实质细胞发生不同程度的变性（水泡变性和颗粒变性）和坏死。电镜下,主要表现为细胞器结构破坏。结果表明,硝酸铅和氯化镉联合急性毒性为相加作用,对大鼠肝脏、肾脏和脾脏均有不同程度的急性毒性损伤。 相似文献
17.
Immunization against intestinal bacterial endotoxin prevents alcoholic fatty liver in rats 总被引：4，自引：0，他引：4
Nishimura G Nakahara K Misawa N Muranaka M Uchida K Kuroda H Murakami N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(3):275-280
Accumulating evidences indicate that an endotoxin originating from intestinal gram-negative bacteria may be involved in alcohol-induced liver injury including fatty liver. Therefore, whether immunization against intestinal bacterial endotoxin blocked fatty liver induced by chronic alcohol and diet including much-unsaturated fatty acid was investigated in rats. The titer of antibody against the endotoxin increased significantly after 13 weeks of continuous immunization. Daily alcohol treatment was initiated at 12 weeks and continued for 4 weeks. Plasma glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT) and triglyceride (TG) levels increased significantly in non-immunized rats receiving alcohol, but not in immunized rats. Continuous alcohol treatment gradually decreased the survival rate to 60% from 13 days after beginning administration in non-immunized, but not immunized, rats. A histochemical study revealed that continuous treatment with alcohol and unsaturated fatty acids caused fatty liver in non-immunized, but not immunized, rats. This study strongly supports the hypothesis that alcohol-induced fatty liver is due to a circulating endotoxin, and suggests that immunization for endotoxin prevent the alcoholic fatty liver. 相似文献
18.
19.
20.
HAN Jian-xin HE Jian-bin GAO Feng YANG Shu-hua LIANG Tian-tian DONG Shuang ZHANG Yi LONG Miao 《中国畜牧兽医》2016,43(2):402-406
The experiment was conducted to study whether the proanthocyanidin (PC) had the protective effects on the liver and kidney oxidative damage induced by zearalenone in mice.40 8 to 9 week-old healthy clean grade Kunming mice with the 45 g body weight were chosen and randomly divided into four groups: control group was given normal saline,PC group was given 100 mg/kg PC,ZEA group was given 40 mg/kg ZEA and PC＋ZEA group was fed 100 mg/kg PC＋40 mg/kg ZEA.Liver and blood sampls were taken after cervical vertebra to death.AST、ALT、MDA、SOD were measured to determine the degree of liver damage and antioxidant capacity,and UA and BUN were used to determine the kidney damage.The results showed that: Compared with the control group,the indexes of PC group were not significant changed (P>0.05),indicating that there was no obvious oxidative damage in the liver and kidney.Compared with control group,ALT,MDA and AST content were significantly or extremely significant increased in ZEA group (P<0.05;P<0.01),SOD content was extremely significant decreased (P<0.01),UA and BUN were extremely significant increased in serum (P<0.01),indicating that the liver and kidney of ZEA group had a serious oxidative damage.The indexes of the PC＋ZEA group (except SOD)were significantly or extremely significant lower than ZEA group (P<0.05;P<0.01),indicating that the oxidative damage of the liver and kidney in PC＋ZEA group was lower than that in the ZEA group.In conclusion,the liver and kidney oxidative damage of mice induced by ZEA was eased to some extent by PC. 相似文献