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为了解犬狂犬病灭活疫苗的免疫抗体水平,作者采用间接ELISA方法对犬狂犬病抗体水平进行跟踪监测,分别采取成年犬免疫前及免疫后7、14、21 d时血清进行狂犬病抗体水平检测。结果表明,成年犬在免疫前及免疫后7、14、21 d时,抗体均值均高于0.5 IU/mL的国际标准,而且在免疫后21 d内,抗体水平随着时间的推移而递增。 相似文献
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为了做好现浇混凝土结构的后浇带施工,笔者着从后浇带的形式,正确理解后浇带的途径及设计要点、施工要点几个方面进行简要阐述。 相似文献
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H5亚型禽流感DNA疫苗质粒pCAGGoptiHA5对商品蛋鸡的免疫保护效果 总被引：2，自引：0，他引：2
将密码子及表达载体优化的H5亚型禽流感DNA疫苗质粒pCAGGoptiHA5以50μg和10μg剂量单次或加强免疫5周龄海兰褐蛋鸡。单次免疫后3周及加强免疫后2周,用100LD50高致病力禽流感病毒A/Goose/GuangDong/1/96(H5N1)[Gs/GD/1/96(H5N1)]鼻腔途径进行攻击,观察发病与死亡情况,并分别于攻毒后3、5、7d采集喉头及泄殖腔拭子进行病毒分离、滴定检测排毒情况,同时检测免疫后、攻毒前及攻毒后血清HI抗体、AGP抗体的动态变化。结果表明,pCAGGoptiHA5以10μg加强免疫后,可以诱导商品蛋鸡产生较高水平的HI抗体,并可保护免疫蛋鸡不发生高致病性禽流感病毒攻击后的死亡。 相似文献
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检疫。宰前、宰后及屠宰过程中,对生猪及猪产品所实施的疫病检验。分宰前检疫,宰后检验（疫）两个环节。宰前检疫指即将屠宰的生猪在屠宰前实施的临床检查。目的在于及时发现病猪或疑似病猪,实行病健隔离,防止疫病散布。及时发生控制部分在宰后检验难以发现或检出的人畜共患病,及时检出一些国内尚未发现的疫病。宰后检疫指生猪屠宰后,对其胴体及各部位组织、器官,依照规程及有关规定进行的疫病检验,是屠宰检疫中最重要的环节。 相似文献
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《中国兽医杂志》2016,(8)
为了研究乳化异氟醚及异氟醚对犬血清中C反应蛋白(CRP)及总补体(CH50)含量的影响,将40只犬随机均分为乳化异氟醚复合制剂麻醉组(RYM组)、异氟醚麻醉组(YM组)、乳化异氟醚复合制剂麻醉+手术(RYMS)及异氟醚麻醉+手术(YMS)四组。RYM、YM两组只进行麻醉不进行手术,RYMS、YMS两组在实施麻醉的同时分别进行肠管吻合术,在麻醉前(T0)、手术完成即刻(T1,而RYM和YM组在麻醉后35~40 min进行采样)、术后24 h(T2)、术后48 h(T3)、术后72 h(T4)等时间点,采血,测定血清中CRP及CH50含量。结果表明,乳化异氟醚复合麻醉及麻醉下手术对犬血清中CRP、CH50含量的影响,与异氟醚相似,两者无明显的差异。 相似文献
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文章对牛、羊、鹅三种常见禽畜夹竹桃中毒后的症状、发病机制及治疗措施进行阐述,以期为兽医站对动物夹竹桃中毒后的诊治提供理论依据。 相似文献
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S Waage 《Nordisk veterinaermedicin》1984,36(9-10):273-281
Results from a clinical study involving 540 cows which recovered after one treatment for hypocalcaemia, 159 cows which recovered after two treatments for hypocalcaemia, and 61 cows which recovered after at least three treatments for hypocalcaemia, showed that the number of treatments before recovery was significantly related to the following factors: Plasma Ca, plasma Mg, packed cell volume, time of first treatment in relation to calving, season of the year, previous milk fever history, management system and the cow's body condition. 相似文献
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1. To determine the effects of heat production on thermoregulatory physiological responses, 5 chickens were forced to walk to increase heat production. The study consisted of two experiments. One involved 3 walking speeds (8, 16 and 24 m/min) at 25°C ambient temperature. The other involved a single walking speed of 16 m/min at 3 ambient temperatures (20, 25 and 30°C). Heat production, heart rate, abdominal temperature, shank skin temperature and respiration rate were measured before, during and after walking.
2. Heat production, heart rate and respiration rate increased abruptly after the start of walking and with increase in walking speed. At the start of walking, an abrupt increase in abdominal temperature occurred independently of walking speed and environmental temperature; then, as walking continued, abdominal temperature increased further with increasing walking speed and increasing environmental temperature. Shank skin temperature declined at the initiation of walking, then increased.
3. Heat production and heart rate recovered rapidly after walking, while shank skin temperature and respiration rate increased and then recovered, except at 30°C.
4. A proportion, 17% to 35%, of the increased heat production was stored, increasing body temperature during walking. After walking, the stored heat was lost by means of panting until abdominal temperature recovered to 41°C or shank skin temperature recovered to 37°C.
5. These results suggest that the greatly‐increased heat production attributable to forced walking leads to abrupt thermoregulatory physiological responses, and the heat stored in the body is actively dissipated by panting until abdominal temperature recovers to the pre‐walking level. The chief mode of heat loss of birds changes with deep body temperature. 相似文献
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Eight heifers were artificially inseminated in the uterine body with 160×106 spermatozoa frozen in French mini-straws. The heifers were slaughtered 2 (n = 4) or 12 (n = 4) h after insemination and spermatozoa were recovered by flushing defined segments of the reproductive tract. The efficiency of the method was checked in different ways. There was a slight underestimation of the number of recovered spermatozoa. This underestimation was randomly distributed among heifers and genital tract segments.The total number of spermatozoa recovered was higher at 2 than at 12 h (14.6 vs 0.6 % of the total number inseminated). Most spermatozoa were found in the vagina both at 2 and 12 h after insemination and in greater number at 2 h. In uterus there was a slight decline in the number of spermatozoa recovered at 2 versus 12 h after insemination. The number of spermatozoa recovered from the oviducts were similar at 2 (89.6 × 103) and 12 h (71.5 × 103) after insemination. At 2 h spermatozoa were found in all parts of the oviduct with the majority located in the utero tubal junction, whereas at 12 h the most were recovered from isthmus.More spermatozoa were recovered from the left than from the right side of the tract in 6 of the 8 heifers. Only in 1 heifer were the majority of spermatozoa found in the oviduct ipsilateral to the follicle bearing ovary. 相似文献
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OBJECTIVE: To determine concentrations of tritiated polysulfated glycosaminoglycans (3H-PSGAG) in serum, urine, and the superficial digital flexor tendon (SDFT) of rabbits after IM administration and molecular weight of 3H-PSGAG recovered from the SDFT. ANIMALS: Twenty-five 12-week-old New Zealand White rabbits. PROCEDURE: Rabbits were given a single dose of 3H-PSGAG (1.1 mg/kg [70 mCi of specific activity/kg] of body weight) IM. At each of 5 sample times (2, 24, 48, 96, and 192 hours), 5 rabbits were randomly selected and sedated, and blood and urine samples were collected. Rabbits were then euthanatized, and the SDFT were immediately harvested from the hind limbs. Scintillation spectrometry was used to detect concentration of 3H-PSGAG in fluid and tissue samples. Gel-filtration chromatography was used to determine molecular weight of recovered 3H-PSGAG. RESULTS: Mean concentrations of 3H-PSGAG in SDFT, serum, and urine were greatest 2 hours after administration. Tritiated PSGAG could be detected in all samples collected 192 hours after administration. Gel-filtration chromatography confirmed that 3H-PSGAG detected in SDFT samples was high molecular weight PSGAG. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicate that PSGAG is distributed to the SDFT, serum, and urine after IM administration in rabbits. Further study is needed to determine whether the same is true in horses and to determine what effect, if any, PSGAG has on inflammation of the SDFT. 相似文献
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Early migration of immature Fasciola hepatica and associated liver pathology in cattle 总被引：1，自引：0，他引：1
Immature Fasciola hepatica were successfully recovered from the body cavity of cattle from two to 21 days after oral infection. Flukes had penetrated the liver in large numbers by day 7 but there did not appear to be a corresponding reduction in the numbers recovered from the body cavity. Liver pathology showed a typical progressive tissue disruption with a neutrophil and lymphocyte infiltration by day 7 and a marked eosinophil response from day 14. The mesothelium of the liver capsule underwent metaplasia, becoming ultimately highly active and columnar. 相似文献
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O O Barriga F Andujar 《Journal of the American Veterinary Medical Association》1988,192(12):1743-1744
A dog with chronic dirofilariasis (Dirofilaria immitis) was given 15 doses of 12 or 24 mg of levamisole/kg of body weight in 6 treatments within 96 days. Concentration of microfilariae in the blood was determined before and after treatment, and Aedes triseriatus mosquitoes were fed on the dog 1 to 5 days after each treatment and examined for D immitis larvae. Several adult worms were recovered from the dog 160 days after the end of the treatments. 相似文献
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An experiment was made to determine the absorption of purine metabolites in dietary nucleic acids through the digestive tract, and also to determine the utilization of nucleic acids absorbed in the body, using growing lambs. Two pairs of 120‐ and 180‐day‐old twin female lambs with a bodyweight of 18.2–19.0 kg were kept in metabolism crates and fed on purine‐free milk replacement (MR) with supplements of exogenous purine (purine base or purine nucleoside) at a level of 0.2 mmol/BW0.75/d for 5 consecutive days, and thereafter they were maintained in the crates for 4 days. The daily amount of exogenous purine supply was calculated based on the urinary excreted purine derivatives (PD) in lambs fed on milk replacement alone. A urine sample was collected daily for 9 consecutive days, and the urinary excretion of PD was determined daily. Urinary PD excretion opened to increase within 24 h after the dose of purine bases, and the level was recovered on 3 days after ceasing the exogenous purine supply. The recovery of PD in the urine was about 70% of the purine supplement. When purine nucleosides were added to the feed, urinary PD excretion was initiated within 24 h after dosing, and the values were recovered after ending the purine nucleoside supply. The recovery rate of PD in the urine was only 30% of the supplemented purine. The plasma allantoin levels were almost similar after feeding purine bases and purine nucleosides, and the values were mostly in the range (40–60 µmol/L). These findings indicate that an exogenous purine can be directly incorporated into the body, and the purine as nucleoside is more effectively utilized for the synthesis of nucleic acids than as a purine base in the body of growing lambs. 相似文献
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A six-year-old intact male Pomeranian was examined because of right eye discomfort. An iris neoplasm was suspected and the eye was enucleated. A uveal melanoma with malignant features was diagnosed. The dog recovered uneventfully from surgery. A general physical examination was performed at 3-month intervals afterwards without any detectable problem, but 18 months after the first presentation the dog suffered a rapid, progressive paraplegia. Radiographic examination and myelography revealed a spinal cord compression at the level of the 8th thoracic (T8) vertebral body. Surgical exploration of the area revealed a potential vertebral neoplasm: histopathology confirmed a melanoma which was suspected to have resulted from metastasis from the previously diagnosed uveal melanoma. 相似文献
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Horstman CL Eubig PA Cornell KK Khan SA Selcer BA 《Journal of the American Animal Hospital Association》2003,39(1):47-51
A 2-year-old, male, mixed-breed dog presented with a 12-day history of vomiting, depression, and weight loss after ingestion of industrial-strength wood glue containing diphenylmethane diisocyanate as its active ingredient. A diagnosis of gastric foreign body was made from survey abdominal radiographs. A large aggregate of solidified wood glue was surgically removed, and the dog recovered uneventfully. Fourteen other cases have been reported to the Animal Poison Control Center at the American Society for the Prevention of Cruelty to Animals (ASPCA). Eight of those 14 cases required surgical intervention. All cases recovered completely. 相似文献