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研究采用DNA池直接测序法寻找边鸡催乳素基因外显子5区域的多态位点。用PCR-RFLP技术检测发现的Taq I位点在边鸡以及3个对照鸡品种(京海黄鸡、尤溪麻鸡和AA鸡)中的单核苷酸多态性,并与边鸡的繁殖性状进行相关性分析。结果表明:在外显子5区域检测到3个突变位点(C5749T、T5821C、C5956T)。T5821C位点经Taq I酶切显示3种基因型(AA、AB和BB)。在4个鸡品种中都以A等位基因为优势等位基因。最小二乘分析表明,边鸡3种基因型个体的繁殖性状无显著差异(P>0.05)。 相似文献
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采用PCR-RFLP和DNA测序技术,研究京海黄鸡、AA鸡、尤溪麻鸡、如皋草鸡、鹿苑鸡、边鸡6个品种IGFBP-3基因第2外显子Msp Ⅰ位点的多态性.结果显示,IGFBP-3基因第2外显子1087 bp处有C→T的突变,鸡群体中表现出AA、AB和BB 3种基因型.3种基因型在6个鸡品种间分布趋势一致:AA为优势基因型,BB为劣势基因型.经x2适合性检验,除京海黄鸡外其余5个品种的Msp Ⅰ位点均处在Hardy-Weinberg平衡状态(P＞0.05).群体遗传分析表明,AA鸡纯合度最高,京海黄鸡纯合度最低.6个鸡品种MspⅠ位点为中度多态.京海黄鸡有效等位基因数最多,为1.900,AA鸡有效等位基因数最少,为1.471.表明鸡IGFBP-3基因在不同品种中具有丰富的单核苷酸多态性,可以进一步作为候选基因来分析其与生长性状的相关性. 相似文献
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京海黄鸡IGFBP-1基因遗传多态性及其与生长性状的关联分析 总被引：1，自引：1，他引：0
以京海黄鸡、AA鸡、尤澳麻鸡、边鸡等4个鸡品种为研究对象,采用PCR-SSCP方法检测胰岛素样生长因r结合蛋白1(insulin-Like growth factor-binding protein-1,IGFIIP 1)第4外显子的多态性,并分析其对京海黄鸡生长性能的遗传效应.结果显示,在IGFBP-1第4外显子区检测到2处变异(5550T→C,5692AAT插入).对于P3扩增片段,在尤溪麻鸡群体中检测到AA、AB、BB 3种基因型,在AA鸡和边鸡品种中检测到AA和AB 2种基因型,京海黄鸡中只检测到AA基因型;对于P4扩增片段,在4个鸡品种中均检测到CC、CD、DD 3种基因型.最小二乘分析结果表明,京海黄鸡3种基因型个体的初生重存在显著差异(P<0.05). 相似文献
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采用PCR-SSCP技术检测边鸡及2个对照鸡群体(京海黄鸡和尤溪麻鸡)肌肉生长抑制素(myostatin,MSTN)基因外显子3的单核苷酸多态性,并与边鸡的生长性状进行相关性分析。结果表明,在MSTN基因外显子3的8527—8528 bp处检测到一个TC的插入。在边鸡和京海黄鸡中只检测到2种基因型(AA和AB基因型),在尤溪麻鸡中检测到3种基因型(AA、AB和BB基因型)。在3个鸡品种中都以A等位基因为优势等位基因。最小二乘分析结果表明,边鸡2种基因型个体的6~16周龄体重均无显著差异(P>0.05)。 相似文献
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以京海黄鸡、AA鸡、尤溪麻鸡、边鸡等4个鸡品种为研究对象,采用PCR-SSCP方法检测信号转导和转录激活子5b(STAT5b)第7外显子和部分内含子7的多态性,并分析其对京海黄鸡体重和繁殖性状的遗传效应。结果显示,在STAT5b第7内含子区域检测到1处突变(8 066bp C→T)。在4个鸡品种中均检测到AA、AB、BB 3种基因型,χ2检验结果表明,除京海黄鸡外其余3个品种群体在该座位均达到Hardy-Weinberg平衡(P>0.05)。最小二乘分析表明,京海黄鸡3种基因型个体的出生重和开产日龄存在显著差异(P<0.05)。 相似文献
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本研究采用PCR-RFLP技术检测边鸡类胰岛素生长因子结合蛋白-3(insulin-like growth factor binding proteins-3,IGFBP-3)基因外显子2 MspⅠ位点的单核苷酸多态性,并与边鸡的繁殖性状进行相关分析。结果表明,在边鸡IGFBP-3基因的1087 bp处检测到1个C→T的突变,显示3种基因型(CC、CT和TT),C等位基因为优势等位基因。最小二乘分析结果表明,CT基因型的开产体重显著高于CC基因型(P<0.05)。不同基因型对其余4个繁殖性状(开产日龄、开产蛋重、300日龄产蛋数及300日龄蛋重)均无显著影响(P>0.05)。 相似文献
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MSTN基因外显子3的多态性及其与边鸡生长性状的关联分析 总被引：2，自引：0，他引：2
采用PCR-SSCP技术检测边鸡及2个对照鸡群体(京海黄鸡和尤溪麻鸡)肌肉生长抑制素(myostatin, MSTN)基因外显子3的单核苷酸多态性,并与边鸡的生长性状进行相关性分析。结果表明,在MSTN基因外显子3的8527—8528 bp处检测到一个TC的插入。在边鸡和京海黄鸡中只检测到2种基因型(AA和AB基因型),在尤溪麻鸡中检测到3种基因型(AA、AB和BB基因型)。在3个鸡品种中都以A等位基因为优势等位基因。最小二乘分析结果表明,边鸡2种基因型个体的6～16周龄体重均无显著差异(P>0.05)。 相似文献
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本研究通过检测鸡NLRC5基因编码区域单核苷酸多态位点,以期分析与免疫性状相关的遗传标记。采用PCR-SSCP方法对3个鸡品种NLRC5基因编码区SNPs进行检测,仅在外显子8区域发现多态位点,其中在文昌鸡中检测到AA、BB和AB 3种基因型,如皋鸡中检测到AA和AB 2种基因型,安卡鸡中仅存在AA基因型。序列分析结果显示,NLRC5基因外显子8存在G43A同义突变。测定部分免疫性状,分析不同鸡品种之间以及不同基因型与免疫性状间的关联性,结果表明:安卡鸡H/L值、ND抗体滴度、IL-1浓度均显著高于如皋鸡和文昌鸡(P0.05),AI抗体滴度极显著低于其他2个品种(P0.01),如皋鸡SBRC抗体滴度最高,但3个品种之间差异不显著(P0.05);不同基因型同免疫性状之间,AA型个体各项性状值均优于AB和BB型个体,但是差异不显著(P0.05)。综合分析,中国地方品种文昌鸡、如皋鸡的抗性优于安卡鸡种,AA型可能是NLRC5基因在机体免疫应答能力的有利基因型。 相似文献
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为了寻找与边鸡生长性状和繁殖性状相关的遗传标记,本试验采用PCR-RFLP技术检测边鸡类胰岛素生长因子Ⅰ受体（insulin-like growth factor Ⅰreceptor,IGF-ⅠR）基因Alu Ⅰ位点的多态性,并分析该多态位点对边鸡生长性状和繁殖性状的遗传效应。结果显示,边鸡IGF-ⅠR基因Alu Ⅰ位点存在多态性,在外显子2的376 bp处有1个G→A突变,产生了GG、GA和AA 3种基因型,基因型频率分别为0.687、0.296和0.017,GG为优势基因型;G等位基因频率为0.835,为优势等位基因。卡方适合性检验结果表明,该基因座位在边鸡群体中处于哈代-温伯格平衡状态（P>0.05）。相关分析结果表明,在8、14、16和18周龄时,GG基因型个体的体重显著高于GA基因型（P<0.05）,GG基因型个体的开产体重极显著高于GA基因型个体（P<0.01）,但并不能说明该位点可以作为影响边鸡繁殖性状的遗传标记。IGF-ⅠR基因外显子2的Alu Ⅰ位点（G→A）可能是影响边鸡生长性状的一个遗传标记。 相似文献
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不同鸡品种TYR基因遗传变异分析 总被引：3，自引：0，他引：3
采用PCR-SSCP方法,对中国宁夏固原鸡、海南文昌鸡、西藏藏鸡和国外引进海赛克斯鸡TYR基因5′侧翼区及外显子13个位点（P1、P2、P3）的遗传变异进行研究。结果表明,P2位点无多态,P1位点在3个中国鸡品种中均出现3种基因型,分别为AA型、BB型和AB型;在引进的海赛克斯鸡中,只检测到BB型和AB型2种类型。4个鸡品种在该位点均处于Hardy-Weinberg平衡状态;P3位点在中国3个鸡品种中均检测到3种基因型,分别为AA型、BB型和AB型,而海赛克斯鸡中只检测到AA和AB型,固原鸡和文昌鸡在该位点处于Hardy-Weinberg平衡状态,藏鸡和海赛克斯鸡均处于极不平衡状态。进一步说明,中国地方鸡品种的TYR基因蕴藏丰富变异资源。经克隆测序分析,P1位点存在C→T的单碱基替换突变,P3位点存在G→A的单碱基替换突变,但未引起氨基酸突变。独立χ2分析显示,两位点基因型和等位基因分布在不同鸡品种之间存在显著或极显著差异（P〈0.05和P〈0.01）,提示TYR基因的基因型和等位基因分布与鸡品种因素显著相关。本研究为以后对鸡TYR基因5′侧翼区及外显子1的进一步研究提供基础依据。 相似文献
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选择中国大陆最早分离的H9N2亚型禽流感病毒(avian influenza virus,AIV)A/Chicken/Guangdong/SS/94(H9N2)(缩写为SS株)和1998年大流行时期分离的H9N2亚型AIVA/Chicken/Shanghai/F/98(H9N2)(缩写为F株)为研究对象,对其在SPF鸡体内的复制能力和传播途径特性比较后发现,F株在4周龄SPF鸡气管中的复制能力高于SS株,F株可以经气溶胶传播途径传播,SS株不能经气溶胶传播途径传播;利用反转录-聚合酶链反应(RT-PCR)方法获取F株和SS株的HA和NA基因的cDNA,序列分析得知,F株和SS株的HA和NA基因的同源性分别是96.6%和98.1%;HA基因的裂解位点氨基酸序列都是PARSSR↓GL,但有5个氨基酸的差异,即166位N(F)→D(SS)、198位A(F)→V(SS)、217位V(F)→I(SS)、335位G(F)→R(SS)、504位L(F)→S(SS);2株病毒的NA基因在63~65位都存在氨基酸缺失,但在NA基因红细胞吸附位点的氨基酸序列不同,分别是IKKDSRSG(F)和IKEDLRSG(SS)。F株和SS株的传播特性差异是否与其表面基因序列有关,有待进一步研究。 相似文献
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禽类的起源、演化及我国主要家禽品种类型与分布 总被引：1，自引：1，他引：0
家禽是重要经济价值动物.本文从禽类种群进化学说出发,简介了禽类的起源、演化、动物学分类和家禽的驯化(养)与品种的形成,并对我国主要家禽(鸡、鸭、鹅)地方品种和培育品种(配套系)的分布与类型作了描述,以期为研究我国家禽起源系统,保护与利用我国家禽品种,促进家禽生产可持续发展提供参考. 相似文献
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近年以来,由于市场因素的刺激,生猪的存养量大幅上升,再加上由于流通环节较多,流通非常频繁,流通距离越来越远。这对繁荣经济,增加养殖效益起了重要的推动作用,但也同时给疾病的感染和传播创造了有利条件,给猪病的防治带来了困难。有的猪场感染了传染病后,由于治疗不及时不得法,而造成了惨重的经济损失。2008年7月中旬,我街道一养猪户因盲目从外地购进中猪,发生猪病疫情,引起猪只连续死亡,造成一定的经济损失。根据流行病学、临床症状、剖检变化和实验室诊断,诊断该病为猪链球菌病和猪伪狂犬病混合感染,现报告如下。 相似文献
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１前言１．１鸡白冠病鸡白冠病是由卡氏住白细胞原虫寄生于鸡的红细胞和单核细胞而引起的鸡的贫血性疾病。吸血昆虫蚋和库蠓叮咬鸡引起传播，是主要的传播媒介，一般在夏末和秋季多发，由于夏季降雨量较大，部分沟渠积水，库蠓和蚋多孳生，因此在多雨水涝的年份发病率明显增高。１９９８年中国从南到北发生洪涝灾害，吸血昆虫的孳生格外严重，出现了一个白冠病多发年，而后两年发病稍轻，并有地区性，今年８月中旬以来白冠病的发病呈抬头趋势，有一定的死亡率，对蛋鸡产蛋率也会引起一定程度的降低，应引起养鸡户的重视。１．２鸡痘鸡痘也是… 相似文献
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Pinard CL Weiss ML Brightman AH Fenwick BW Davidson HJ 《American journal of veterinary research》2003,64(1):104-108
OBJECTIVE: To evaluate lactoferrin and lysozyme content in various ocular glands of bison and cattle and in tears of bison. SAMPLE POPULATION: Tissues of ocular glands obtained from 15 bison and 15 cattle and tears collected from 38 bison. PROCEDURE: Immunohistochemical analysis was used to detect lysozyme and lactoferrin in formalin-fixed, paraffin-embedded sections of the ocular glands. Protein gel electrophoresis was used to analyze ocular glands and pooled bison tears by use of a tris-glycine gel and SDS-PAGE. Western blotting was used to detect lactoferrin and lysozyme. RESULTS: Immunohistochemical staining for lactoferrin was evident in the lacrimal gland and gland of the third eyelid in cattle and bison and the deep gland of the third eyelid (Harder's gland) in cattle. Equivocal staining for lactoferrin was seen for the Harder's gland in bison. An 80-kd band (lactoferrin) was detected via electrophoresis and western blots in the lacrimal gland and gland of the third eyelid in cattle and bison, Harder's glands of cattle, and bison tears. An inconsistent band was seen in Harder's glands of bison. Lysozyme was not detected in the lacrimal gland of cattle or bison with the use of immunohistochemical analysis or western blots. Western blots of bison tears did not reveal lysozyme. CONCLUSIONS AND CLINICAL RELEVANCE: Distribution of lactoferrin and a lack of lysozyme are similar in the lacrimal gland of cattle and bison. Differences in other tear components may be responsible for variability in the susceptibility to infectious corneal diseases that exists between bison and cattle. 相似文献
19.
Harvey CE 《Journal of veterinary dentistry》2002,19(4):186-195
Crown width, height and buccal surface areas were measured on heads or skulls of four dogs and four cats, and were compared with similar measurements on models of human dentition. Buccal surface area variability was greater in dogs and cats than in humans, and teeth of cats were smaller. Horizontal (gingival and occlusal halves) and vertical (mesial, middle, and distal thirds) buccal surface area variability was also greater in canine and feline teeth compared with human teeth. This increased variability suggests the need for testing of reliability and repeatability of scoring when using plaque and calculus indices based on horizontal or vertical segmentation. Buccal surface area variability between teeth also prompts questioning the validity of equal weighting of smaller, irregularly-shaped teeth when calculating a mean mouth score. Whether equal or more reliable results would be obtained from scores of whole teeth in comparison with segmentation indices used currently has yet to be determined. 相似文献
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Over a period of about 12 years, 30 abnormal Schistosoma mattheei cercariae were found among a total of approximately 2.8 million examined. Initially seven were recovered from about 1.02 million (0.0007%), which were examined individually while being counted with the aid of a stereoscopic microscope. Subsequently, on the strength of relatively high percentages of abnormal individuals recovered when counting cercariae that failed to penetrate into oxen, it appeared that the morphologically abnormal cercariae were unable to swim and would mostly sediment out of a suspension while most of the normal cercariae would remain swimming. This surmise is supported by recovery of 23 morphologically abnormal cercariae (0.001%) from about 1.8 million, by examining the sediment after the cercarial suspension had been left standing undisturbed in glass measuring cylinders. The abnormalities ranged from aberrant tails only (e.g. an underdeveloped tail, or different degrees of schism) or aberrant heads only, to abnormalities of both the heads and tails. A suggested schematic classification of abnormal cercariae is presented. A young, adult hamster was exposed to eight S. mattheei cercariae with complete schism of the shaft of the tail, by pipetting the cercariae onto the shaved abdominal skin of the anaesthetised animal. Two underdeveloped females were subsequently encountered in squash preparations of the liver when the hamster was killed for worm recovery 10 weeks after infection, thus showing that some of the abnormal cercariae were viable. A method is also described for killing and fixing cercariae while retaining some of the shining brilliance of live cercariae, without them becoming shrivelled, granular and semi-opaque, as occurs when cercariae die spontaneously or are killed with heat. This is apparently the first report of abnormal cercariae of S. mattheei. In addition, a method of concentrating abnormal cercariae after emergence from a snail, a schematic classification of abnormal cercariae and a method for killing and fixing cercariae while retaining much of the shiny brilliance of live cercariae are also reported for the first time as far as is known. 相似文献