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1.
Abstract.— Amino acid profiles of freshwater prawn Macrobrachium rosenbergii were determined during early larval stages (I-IX) to provide some baseline biochemical information of the growing larvae. The larvae obtained from several females were stocked into three 250-L tanks at a density of 30 larvae/L. The feeding regime consisted of newly hatched Artemia nauplii and egg custard containing 5% cod liver oil. For each developmental stage, larval samples from each experimental tank were pooled, freeze dried and after acid hydrolysis, the quantification of amino acids was done by a reverse phase high performance liquid chromatography ( N = 2). The highest represented of the total amino acids were for glutamic acid and phenylalanine (with cystine) with ranges from 13.4–16.6 and 9.7–11.5%, respectively, whereas tryptophan (1.4–1.6%). methionine (1.4–2.7%) and histidine (2.9–4.2%) were relatively lower. The levels of the following essential amino acids did not significantly change during larval development: arginine, leucine, phenylalanine (plus cystine), threonine, tryptophan and valine. Despite statistically significant changes that were observed in levels of some amino acids, amino acid composition appeared to be relatively unchanged during the larval growth. The results may suggest that the amino acid requirements of the freshwater prawn is relatively constant during larval life and can be satistied by a suitable protein source that resembles the larval amino acid profile.  相似文献   

2.
在禁食胁迫下,对在盐度为0(S0)和30(S30)水体中的凡纳滨对虾肌肉游离氨基酸(FAA)的浓度变化进行测定.结果表明,禁食15d后,两种盐度中的凡纳滨对虾肌肉游离氨基酸总量均有所升高,但其中游离的牛磺酸(Tau)、苏氨酸(Thr)、脯氨酸(Pro)、丙氨酸(Ala)、半胱氨酸(Cys)、缬氨酸(Val)、蛋氨酸(Met)、异亮氨酸(Ile)、亮氨酸(Leu)、苯丙氨酸(Phe)、赖氨酸(Lys)、组氨酸(His)的浓度却有不同程度的降低,脯氨酸(Pro)在禁食15d后的浓度降为0,牛磺酸(Tau)、苏氨酸(Thr)、丙氨酸(Ala)、半胱氨酸(Cys)、苯丙氨酸(Phe)、赖氨酸(Lys)的降幅也比较显著(P<0.05);但甘氨酸(Gly)、天门冬氨酸(Asp)、酪氨酸(Tyr)、精氨酸(Arg)等主要游离氨基酸的浓度却显著升高,从而使游离氨基酸总浓度升高.实验表明,凡纳滨对虾肌肉里游离氨基酸浓度在禁食胁迫下降解代谢强度不一,饲料中添加脯氨酸(Pro)、精氨酸(Arg)和赖氨酸(Lys)等游离氨基酸后,可能会对凡纳滨对虾的生长起到促进作用.  相似文献   

3.
Accurate estimates of maintenance requirement of amino acids are important for feed formulation. However, very little is known about maintenance requirements of essential amino acids in fish. A nonlinear mixed modelling approach was employed in this study to estimate maintenance requirements of essential amino acids of fish from published studies. The maintenance requirements of arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan and valine were estimated to be 7.7 (±6.6), 9.8 (±2.4), 6.4 (±24.8), 9.1 (±13.7), 15.6 (±8.2), 18.4 (±1.9), 14.6 (±3.2), 5.4 (±4.4), 0.5 (±1.3) and 9.3 (±26.6) mg/kg BW0.75/day respectively, whereas the maintenance requirements of these amino acids as a proportion of their total requirements for protein deposition were estimated to be 4.8%, 31.0%, 8.9%, 12.7%, 5.2%, 11.9%, 19.2%, 2.5%, 2.2%, 10.8% respectively. For most of the essential amino acids, the maintenance requirements represent a small proportion of total requirement (below 10%) with notable exceptions for histidine and phenylalanine. Results from this study suggest that maintenance requirements of fish cannot be assumed equal for all amino acids. The results can be used in developing factorial or mechanistic models of amino acid requirement and utilization.  相似文献   

4.
A study was conducted to investigate the muscle amino acid profiles of five species of marine fish, Pseudosciaena crocea (large yellow), Lateolabrax japonicus (common sea perch), Pagrosomus major (red seabream), Seriola dumerili (Dumeril's amberjack) and Hapalogenys nitens (black grunt). These carnivorous fishes, all of which belong to the Perciforms, were sampled from Xiamen Bay. Chemical analysis shows significant difference (P < 0.01) among the five species in the muscle total amino acids (TAA). Lateolabrax japonicus has the highest level of TAA in muscle tissues. Significant difference in muscle total essential amino acid (TEAA) and total non‐essential amino acid (TNEAA) were also found among the five species. Lateolabrax japonicus has the highest TEAA level, and H. nitens has the highest level of TNEAA level. Significant difference (P < 0.01) existed in specific EAA except for lysine (P > 0.05). The significant difference (P < 0.05) of the A/E ratios [(each essential amino acid content/total essential amino acid content including cystine and tyrosine) ×1000] based on the essential amino acid composition of muscle tissue from the five species were found in lysine, histidine, methionine, cystine, valine, leucine, isoleucine, and arginine, except for threonine, phenylalanine and tyrosine. These differences indicate that the amino acids profile is species specific for the five species and their essential amino acid requirements are greatly different although they were fed similar feed. In contrast to the reference amino acid profile recommended by FAO/WHO, muscle proteins of each fish were all rich in lysine, the S‐containing amino acids and threonine, but histidine, valine, leucine, isoleucine, phenylalanine and tyrosine were deficient for children's diets. The first limiting amino acid was histidine for P. crocea and L. japonicus, valine for P. major and H. nitens, leucine for S. dumerili. The amino acid score was 66.8, 76.7, 78.4, 84.0 and 95.7 for P. crocea, L. japonicus, P. major, H. nitens and S. dumerili, respectively. In the adult human diet the muscle protein of the five species of marine fish can almost fulfil the requirements of all essential amino acids except for the histidine of P. crocea and L. japonicus and thus can serve as supplemental source of protein in cereal‐based adults diets if we reinforce the histidine correspondingly. Therefore, increasing the proportion of marine fish in the diet of the people in the area where paddy rice and wheat are the main protein sources is an effective way to enhance the nutrition value of food and improve the nutrition status of the developing countries. Moreover, all of five marine fish species contained a comparatively high content of glutamic acid and arginine, which are beneficial to the patients under such conditions as trauma, burn injury, massive small‐bowel resection and renal failure.  相似文献   

5.
Fish Physiology and Biochemistry - System b0,+ absorbs lysine, arginine, ornithine, and cystine, as well as some (large) neutral amino acids in the mammalian kidney and intestine. It is a...  相似文献   

6.
The changes in proximate composition, amino acid (total and free) and fatty acid content of artificially propagated trout cod, Maccullochella macquariensis larvae from five mothers hatched, weaned and reared separately, each in two groups, one fed with Artemia naupli and the other starved, for 15 days (after yolk resorption), are presented. There was no significant change in the proximate composition of fed larvae with devlopment, but in starved larvae the protein (linearly) and lipid (curvi-linearly) content decreased significantly as starvation progressed. The essential amino acids (EAA) and non- essential amino acids (NEAA) found in highest amounts in trout cod larvae were lysine, leucine, threonine and arginine, and alanine, serine and glutamic acid, respectively. In fed larvae the total amino acid (TAA), TEAA and TNEAA content did not vary significantly as development progressed. In starved larvae the TAA, EAA and NEAA content, as well as all the individual amino acids decreased significantly (P<0.05) from the levels in day of hatch and/or yolk-sac resorbed larvae. The greatest decrease occurred in the TEAA content (7.38±0.76 at day of hatch to 1.96±0.09 15 day starved in moles larva–1; approximately a 74% decrease), whereas the decrease in TNEAA was about 38%. Unlike in the case of TAA distinct changes in the free amino acid (FAA) pool were discernible, from day of hatch and onwards, in both fed and starved trout cod larvae. In both groups of larvae the most noticeable being the decrease of % FEAA in TFAA, but not the % FAA in TAA. Four fatty acids together, accounted for more than 50% of the total in each of the major fatty acid categories in all larvae sampled; 16: 0, 18:1n-9, 22: 6n-3 and 20: 4n-6, amongst saturates, monoenes, n-3 PUFA and n-6 PUFA, respectively. Twelve fatty acids either decreased (14: 0, 16: 1n-7, 20: 1n-9, 20: 4n-6, 20: 5n-3, 22: 5n-3 and 22: 6n-3) or increased (18: 2n-6, 18: 3n-3, 18: 3n-6, 18: 4n-3 and 20: 3n-3) in quantity, after 15 days of feeding, from the base level in day of hatch and/ or yolk- sac resorbed larvae. The greatest increase occurred in 18: 3n-3 from 6.4±0.1 to 106.2±13.1 g mg lipid–1 larva–1, and the greatest decrease occurred in 22: 6n-3 (181.2±12.4 to 81.4±6.2 g mg lipid–1 larva–1). In starved larvae, at the end of 15 days, all the fatty acids, except 18: 0, 20: 3n-3 and 20: 4n-6, decreased significantly (P<0.05) from the levels in day of hatch and/or yolk- sac resorbed larvae.  相似文献   

7.
ABSTRACT: To investigate the effects of disproportionate levels of dietary arginine and lysine on juvenile Japanese flounder Paralichthys olivaceus , growth performance and biochemical parameters were evaluated by feeding five test diets, comprising different levels of arginine and lysine, to triplicate groups of juveniles (initial bodyweight 1.85 g) for 40 days. Crystalline amino acids were supplemented to test diets to correspond to the amino acid pattern found in the whole body protein of the Japanese flounder, except for arginine and lysine. After the feeding trials, plasma arginine, lysine and urea levels, excreted ammonia-N, and liver arginase activity were analysed. Survival, specific growth rate, feed conversion efficiency, and apparent protein retention were adversely affected ( P  <   0.05) by the deficiency in dietary arginine or lysine concentrations. An excess of either lysine or arginine in the diet did not depress growth when the diets contained adequate levels of either arginine or lysine, proving that there is no evidence for an arginine–lysine antagonism. Results for plasma arginine, lysine and urea levels, excreted ammonia, and liver arginase activity also demonstrated that Japanese flounder juveniles are not sensitive to excess dietary levels of lysine and arginine.  相似文献   

8.
Abstract

Amino acid profiles of various body tissues from juvenile and adult discus fish, Symphysodon aequifasciata, were compared. Results from whole-body tissue revealed significant difference (P < 0.05) between the levels of lysine, phenylalanine, alanine, aspartic acid, and serine among the two different fish sizes. Levels of arginine, histidine, leucine, methionine, phenylalanine, and threonine were also higher in muscle tissue of adult fishes. Analysis of gill tissues showed significant difference in levels of histidine, isoleucine, lysine, methionine, threonine, tryptophan, aspartic acid, glutamic acid, proline, and serine were significantly different among the different fish stages. This study also showed that among all ten essential amino acids, muscle had the highest levels of histidine, isoleucine, lysine, and tryptophan; gills had the highest level of arginine; while levels of methionine and threonine were higher in the intestinal tissue. Regression analysis between the calculated whole-body A/E ratio of discus juveniles and with quantitative dietary amino acid requirements of several fish species also showed significant correlation, with highest r values (> 0.90) obtained with tilapia, common carp, channel catfish, and Japanese eel. This profile may be useful as a preliminary reference index to evaluate suitability of various ingredients in terms of amino acid profile for development of economical diet for discus farming.  相似文献   

9.
Three separate experiments were conducted to investigate the utilization of dietary free amino acids by white sturgeon,Acipenser transmontanus. In the first experiment, sturgeon were fed for 8 weeks with isoenergetic and isonitrogenous diets containing either intact protein, an amino acid mixture, or a neutralized amino acid mixture of similar composition. Sturgeon fed the two amino acid diets grew significantly (p<0.05) less, mainly because of reduced feed intake. In the second experiment, postprandial changes in plasma free amino acids of sturgeon force-fed the above diets were monitored at 2, 4, 6, 8, 12, and 24 h Total plasma amino acids in sturgeon force-fed the intact protein diet showed a gradual increase and reached peak levels at 8 h. In contrast, plasma amino acids of fish force-fed the amino acid diets showed a rapid increase and reached maximal levels at 2 h. In the third experiment, free amino acid diets showed a urine of sturgeon force-fed the three diets was monitored at 4, 8, 12, 16, 20, and 24 h. In all dietary groups, peak excretion of indispensable amino acids was detected at 8 h after feeding but with significantly higher levels from fish force-fed the amino acid diets. Sturgeon force-fed the neutralized amino acid diet excreted 35% less amino acids compared to fish force-fed the non-neutralized diet. Sturgeon force-fed the intact protein diet excreted significantly higher amounts of ammonia. Under amino acid feeding conditions, sturgeon excreted more amino acids in its urine, but the urinary excretion of amino acids was not a major route in dealing with the rapid absorption of dietary free amino acids.  相似文献   

10.
饥饿对草鱼血清游离氨基酸的影响   总被引：1，自引：0，他引：1  
游文章  文华  马琳 《淡水渔业》2007,37(3):26-29
将池塘捕获的体重(1.70±0.80)kg的草鱼(Ctenopharyngodon idella)饲养在室内水族箱内,分别测定了空腹(禁食1 d,26样本)、饥饿15 d(15样本)、饥饿30 d(15样本)的草鱼血清游离氨基酸含量。结果显示:草鱼血清游离氨基酸总量基本恒定,空腹、饥饿15 d和饥饿30 d的氨基酸总量无显著差异;处于饥饿状态的草鱼血清游离必需氨基酸总量极显著地高于空腹状态,非必需氨基酸总量极显著地低于空腹状态;血清赖氨酸、缬氨酸、异亮氨酸、亮氨酸和胱氨酸含量因饥饿显著升高,而组氨酸、苯丙氨酸、天冬氨酸、丝氨酸、谷氨酸和酪氨酸因饥饿显著降低;饥饿15 d和饥饿30 d各氨基酸含量均未见差异。草鱼血清游离氨基酸含量在正常摄食情况下主要受饲料蛋白质影响,处于饥饿状态下主要来源于肌体蛋白质的分解,比较正常摄食后和饥饿状态下血清游离氨基酸的组成可评价饲料蛋白质的质量。  相似文献   

11.
ABSTRACT:   For the purpose of clarifying the differences of taste components between the testis and ovary of the sea urchin Anthocidaris crassispina , variations in free amino acid composition in gonadal extract during gonadal development were investigated using chemical and histopathological methods. Sea urchins were collected at Iki Island, Nagasaki, Japan between 30 April and 5 June 2003, and maturation stages and the free amino acid compositions were determined. The sweet amino acids threonine (Thr), glycine (Gly), and alanine (Ala), the umami amino acid glutamic acid (Glu), and the bitter amino acids lysine (Lys) and arginine (Arg) were the main constituents accounting for more than 80% of total free amino acids. In the testis and ovary, Gly levels were the highest among them and increased with gonadal development. The other free amino acids consisted mainly of bitter amino acids and either showed no variation or decreased with gonadal development. These variations in free amino acid composition were observed more clearly in the ovary than the testis. These findings indicate the possibility of differences in the flavor of sea urchin gonad by sex and gonadal development stages.  相似文献   

12.
Metamorphosing leptocephalous larvae of the bonefish (Albula sp.) were analyzed for total ninhydrinpositive substances (NPS) and free amino acids. Total NPS content showed little change during metamorphosis. The average NPS value (±S.E.) for 16 larvae was 1.8 (±0.1) mg×larva–1, which represents approximately 4% and 8% of the total dry weight of early and advanced larvae, respectively. Taurine was the most abundant free amino acid in whole-larva extracts, accounting for 36% and 59% of the total by weight in early and advanced larvae, respectively. The essential amino acids, leucine, isoleucine, phenylalanine, histidine, valine, methionine, lysine and arginine, accounted for about half (47%) of the total in early larvae but were reduced to about 23% of the total in advanced larvae. All of the component essential amino acids decreased during metamorphosis, but the greatest effect was seen with the first five. The remaining non-essential amino acids comprised less than 20% of the total in early larvae and, although the overall value changed little during metamorphosis, certain components such as glycine and glutamic acid showed large increases whereas others such as tyrosine and serine were reduced. Increases in amino acid content after acid hydrolysis of whole-larva extracts indicated that trichloroacetic acid-soluble, low molecular weight peptides were present in both early and advanced leptocephali.  相似文献   

13.
为了研究饥饿对大黄鱼(Larimichthys crocea)幼鱼肌肉中氨基酸和脂肪酸含量的影响,取大黄鱼幼鱼540尾,体重均值为(40.80±3.40)g,分组进行为期0 d(S0)、4 d(S4)、8 d(S8)、12 d(S12)、16 d(S16)、20 d(S20)的饥饿处理,测定背部肌肉的氨基酸和脂肪酸含量。结果显示,测定的大黄鱼背肌的16种氨基酸中,蛋氨酸含量在不同饥饿处理时间之间差异显著(P0.05),饥饿持续8 d其含量达到最小(0.48%±0.12%),16 d时达到最高(1.62%±0.23%);其余15种氨基酸含量的差异均不显著(P0.05),但均表现出随着饥饿时间延长先上升后下降的趋势,在16 d时达到最高,20 d时明显降低。非必需氨基酸、必需氨基酸、呈味氨基酸、鲜味氨基酸及氨基酸总量的变化趋势与上述15种氨基酸一致。在不同饥饿处理组的大黄鱼背肌中脂肪酸含量差异显著(P0.05)。其中,饱和脂肪酸(SFA)含量随着饥饿时间延长呈先升高后下降的趋势,在S8组达到最大,为30.90%±0.28%;单不饱和脂肪酸(MUFA)含量在不同处理组之间差异不显著(P0.05),但所有处理组均稍大于对照组(S0);多不饱和脂肪酸(PUFA)含量则呈先下降后升高的趋势,在S12组时达到最低,其值为31.87%±0.65%。由上可知,通过适当的饥饿处理,可以改变肌肉中氨基酸和脂肪酸含量,从而较好地改善大黄鱼的肉质风味。  相似文献   

14.
The amino acid composition and protein levels of three species of cephalopods (Octopus vulgaris, Loligo gahi and Todarodes sagittatus), the natural diets of common octopus (O. vulgaris) and different kinds of meals were determined in order to optimise the content of these nutrients in artificial feeds. Arginine, leucine and lysine were the most abundant essential amino acids in cephalopods, while glutamate and aspartate represented the main non-essential amino acids. Arginine and leucine were the limiting amino acid in most samples, with maximum Chemical Score values for mussel (79–98 %), squid (84 %) and crustaceans (65–91 %); medium for fish (41–70 %); and minimum for meals (29–64 %). Mussel, squid, crustaceans and fish showed a high essential amino acid index according to Oser (OI: 88–99 %) suggesting a suitable amino acid balance. The protein from animal meals (fish and krill) covered all the essential amino acids except arginine and lysine in fish meal. The vegetable meal presented the worst amino acid balance (OI: 74–89 %) with several deficiencies in essential amino acids, including arginine, threonine, lysine and methionine. Supplementation with arginine or leucine and protein complementation of crustaceans and bivalves with fish or animal meal are proposed as alternatives for improving the performance of protein in feed for cephalopods.  相似文献   

15.
This study tested the hypothesis that different starvation time for fish before blood sampling can influence the result of plasma biochemical analysis. Glucose (GLU), total cholesterol (CHOL), triglyceride (TGL) and total protein (TP) in the plasma of juvenile Amur sturgeon (Acipenser schrenckii) were measured after different starvation duration (0, 0.5, 1, 2 and 3 days). Starvation time had a significant influence on plasma GLU, CHOL, TGL and TP concentrations (P < 0.05). Plasma GLU and TP concentrations were unpredictably variable with duration of starvation. Plasma TGL and CHOL concentrations after 3 days starvation were significantly higher than that in fish starved for 0, 0.5, or 1 day, and no significant difference was found among fish starved for 0, 0.5, or 1 day. In order to get baseline value for these blood parameters, juvenile Amur sturgeon should be starved for 1 day.  相似文献   

16.
摘要:为了给华鲮种质资源的开发利用及人工养殖提供依据,对在池塘人工养殖的华鲮含肉率及肌肉营养成分进行测定,结果表明:含肉率为60.65％;肌肉中蛋白质、脂肪和灰分含量分别为19.99％、5.10％、1.51％;肌肉中18种常见氨基酸总含量(占肌肉干重)为75.23％。其中必需氨基酸含量为33.36％,占氨基酸总量的44.34％。必需氨基酸与非必需氨基酸含量的比值为0.80。鲜昧氨基酸含量为30.07％,占氨基酸总量的37.40％。必需氨基酸指数（WH0／FAO标准为参照）为82.34。色氨酸为第1限制性氨基酸,蛋氨酸+胱氨酸为第2限制性氨基酸。  相似文献   

17.
A 6‐week feeding trial was conducted to estimate the optimum dietary essential amino acid (EAA) pattern for silvery‐black porgy juvenile based on the AA deletion method. Eleven isonitrogenous and isoenergetic diets were formulated containing 60% of fish meal nitrogen and 40% of crystalline AA nitrogen. In the control diet, the EAA profile was made similar to fish meal protein. Ten other diets were formulated similar to the control diet but replacing 40% of each EAA by a mixture of non‐essential amino acids. Triplicate groups of fish (initial body weight of 4.7 g) were handfed with the experimental diets, three times a day, to visual satiation, for 42 days. At the end of the trial, final body weight of all EAA‐deficient groups was lower than that of control group, ranging from 6.3% of reduction with arginine‐deficient diet to 39.4% of reduction with lysine‐deficient diet, relatively to the control group. Based on the relationship between nitrogen retention and EAA intake of the control and EAA‐deficient diets, the optimal dietary EAA profile for silvery‐black porgy juveniles was estimated to be (g 16/g N): arginine 5.3, lysine 6.0, threonine 5.2, histidine 2.5, isoleucine 4.6, leucine 5.4, methionine + cysteine 4.0 (in a diet containing 0.6 cysteine), phenylalanine + tyrosine 5.6 (in a diet containing 1.9 tyrosine), tryptophan 1.0 and valine 4.6.  相似文献   

18.
Using rainbow trout, Oncorhynchus mykiss (Walbaum), of approximately 50 g, a 63 day feeding trial was undertaken to evaluate the efficacy of individual and multiple amino acid supplements in diets where soyabean meal was used as the principle protein source. Iso-nitrogenous diets (45% crude protein) were formulated where the control diet (F) contained fishmeal as the reference protein and a solvent extracted soyabean meal (S) replaced approximately 66% of this protein source. The soyabean containing diets were then supplemented with crystalline amino acids thus; methionine only, dual supplemented with two methionine and lysine levels and finally, a supplement comprising methionine, lysine, tryptophan, threonine, arginine and histidine. The results showed that soyabean meal (SBM) was inferior to the reference protein when SBM was used to replace 66% of the fishmeal and that no significant restoration in growth, feed efficiency and apparent net protein utilization was obtained by either methionine only or dual methionine and lysine supplementation. However, by comparison with the fish fed the unsupplemented, single and dual supplemented diets, multiple amino acid incorporation was associated with significantly improved percentage weight gain, specific growth rate and marginal improvements in apparent net protein utilization. However, performance was not equal to that of the fish fed the fishmeal based control diet. The results are discussed with respect to the level of each of the essential amino acids (EAA) as a proportion of the total EAAs (A/E index) of test diets by reference to the whole body tissue amino acid profile of rainbow trout.  相似文献   

19.
Following the completion of the lecithotrophic phase, most marine larvae rely on an incompletely developed digestive tract to absorb amino acids (AA) and other nutrients needed for rapid growth. Despite their undeveloped state, larvae must absorb nutrients in sufficient amounts to fuel exceptionally high rates of growth. This study examined the ability of larval Atlantic halibut to absorb, assimilate and catabolise dispensable (alanine, glutamate) and indispensable (arginine, lysine) dietary free amino acids (FAA) using tube-fed AA solutions with 14C tracers. Absorption of FAA was rapid with an average of 71% absorbed from the gut within 30 min after tube feeding. Evacuation of FAA by larvae was low, averaging only 6% of tube-fed dose. Dispensable amino acids (DAA) were catabolised in greater proportion (17%) than indispensable FAA (54%). Saturation of FAA transporters was not attained under the present conditions (20 mM, 20% midgut filling). Absorption rates did not differ significantly between the four FAA.  相似文献   

20.
为研究饲料中精氨酸(Arg)、赖氨酸(Lys)水平及其相互作用对大菱鲆生长、体成分和肌肉氨基酸含量的影响,本实验以初始体质量为(18.48±0.16)g的大菱鲆作为研究对象,采用3×3双因素设计,在基础饲料中分别添加Arg(0%、0.9%和2.0%)和Lys(0%、1.19%和2.39%),配制成9种等氮等能的实验饲料,每个处理设3个重复,每重复30尾鱼,养殖周期为8周。实验结果表明,当饲料中Lys添加量为1.19%时,大菱鲆增重率和特定生长率较其他两种添加量组显著升高(P < 0.05),但精氨酸的添加对其影响不显著且与赖氨酸之间不存在交互作用(P > 0.05)。饲料效率、蛋白质效率、蛋白质保留率和鱼体蛋白质含量受饲料中Lys和Arg添加量的交互影响(P < 0.05),在Arg和Lys添加量分别为0.9%和1.19%时,数值最高,显著高于赖氨酸未添加组和高添加组(P < 0.05)。全鱼粗脂肪、水分、灰分和形体指标不受Arg和Lys的交互作用影响(P > 0.05)。粗脂肪和水分随Lys的添加量升高而显著降低(P < 0.05);肝体比和脏体比均随饲料中Arg和Lys添加量的升高而显著降低(P < 0.05)。肌肉中大多数氨基酸含量受饲料Arg和Lys添加量的交互作用,显著性最低值均出现在Arg和Lys添加量分别为0.9%和2.39%组(P < 0.05)。以上结果表明,Arg和Lys的交互作用显著影响了大菱鲆幼鱼的饲料效率、鱼体蛋白质沉积和肌肉氨基酸含量;Arg和Lys添加量分别为0.9%和1.19%时,大菱鲆有最大生长和饲料利用效率;与Arg相比,Lys为主要影响因素,适量添加Lys可以促进生长,而添加量过高Lys会与Arg产生拮抗作用,抑制生长、饲料利用和肌肉氨基酸沉积。  相似文献   

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