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1.
The mode of action of nitric oxide (NO) in inhibiting ethylene biosynthesis and fruit softening during ripening and cool storage of mango fruit was investigated. Hard mature green mango (Mangifera indica L. cv. ‘Kensington Pride’) fruit were fumigated with 20 μL L−1 NO for 2 h at 21 °C and allowed to ripen at 21 ± 1 °C for 10 d, or stored at 13 ± 1 °C for 21 d. During ripening and cool storage, ethylene production and respiration rate from whole fruit were determined daily. The 1-aminocyclopropane-1-carboxylic acid (ACC) content, activities of ACC synthase (ACS), ACC oxidase (ACO), and fruit softening enzymes such as pectin esterase (PE), endo-1,4-β-d-glucanase (EGase), exo- and endo-polygalacturonase (exo-PG, endo-PG) as well as firmness and rheological properties of pulp were determined at two- and seven-day intervals during ripening and cool storage, respectively. NO fumigation inhibited ethylene biosynthesis and respiration rate, and maintained higher pulp firmness, springiness, cohesiveness, chewiness, adhesiveness, and stiffness. NO-fumigated fruit during cool storage and ripening had lower ACC contents through inhibiting the activities of both ACS and ACO in the fruit pulp. NO-fumigated fruit showed decreased activities of exo-PG, endo-PG, EGase, but maintained higher PE activity in pulp tissues during ripening and cool storage. In conclusion, NO fumigation inhibited ethylene biosynthesis through inhibition of ACS and ACO activities leading to reduced ACC content in the fruit pulp which consequently, reduced the activities of fruit softening enzymes during ripening and cool storage.  相似文献   

2.
1-Methylcyclopropene (1-MCP) treatment maintains apple fruit quality during storage, but its efficacy is dependent on a number of conditions. ‘Tsugaru’ apples are a major early season cultivar in Japan, but because ‘Tsugaru’ fruit produce abundant ethylene, they have a short shelf-life, and efficacy of 1-MCP is not as high with ‘Tsugaru’ as with other cultivars. To improve 1-MCP efficacy, ‘Tsugaru’ fruit were pre-cooled at −1 °C or −3 °C for 24 h before 1-MCP treatment. Ethylene production decreased with the cold treatment, resulting in better storage after 1-MCP treatment. Although ethylene production was low at the end of 24 h of the cold pre-treatment, expression of ACS1, the ethylene receptor genes ERS1, ETR1(a), ETR1b, ETR2 and ETR5, and the cell wall degradation-related gene PG1 all increased with a 24 h cold treatment. It is assumed that these elevated gene expression levels were not caused by ethylene, but more directly by cold stimulus. Thus, a short period of cold stimulus suppresses ethylene production, but induces expression of some genes. 1-MCP treatment was more effective with some initial fruit chilling.  相似文献   

3.
Fruit of cv. Gros Michel banana were treated with 1-MCP (1000 nL L−1 for 4 h at 25 °C) and then packed in non-perforated polyethylene (PE) bags for modified atmosphere storage (MAP). The bags were placed in corrugated cardboard boxes and stored at 14 °C. Fruit were removed from cool storage and ripened at room temperature using ethephon. The length of storage life was determined by the change in peel color to yellow, after this ethephon treatment. Fruit treated with 1-MCP + MAP had a storage life of 100 days. The storage life of control fruit (no 1-MCP and no MAP) was 20 days. Fruit held in PE bags without 1-MCP treatment had a 40 day storage life, and the same was found in fruit treated with 1-MCP but without PE bags. 1-MCP is an inhibitor of ethylene action, but also inhibited ethylene production, mainly through inhibition of ACC oxidase activity in the peel. MAP inhibited ethylene production mainly through inhibition of ACC oxidase, both in the peel and pulp. The combination of 1-MCP treatment and MAP storage resulted in much lower ethylene production due to inhibition of both ACC synthase and ACC oxidase activity.  相似文献   

4.
It is now widely accepted that 1-MCP reduces ethylene production and prevents scald disorder in apple skin tissue. However, despite this beneficial effect, very little is known about the effects of 1-MCP on this tissue. This study aimed to determine how this treatment affects ACC metabolism in both skin and pulp tissues in relation to cold storage. Changes in ACC metabolism were monitored in control and 1-MCP treated fruit stored in air and removed after 0, 15, 30, 90 and 150 days of storage. 1-MCP treatment caused an inhibition of ethylene production but also of ACC synthase (ACS) activity and ACC levels both in pulp and skin. Compared to the control, 1-MCP treatment also induced a significant reduction in ACC oxidase (ACO) activity, but the inhibition remained incomplete in both tissues. High levels of MACC were found in 1-MCP treated fruit, showing the presence of a malonyl transferase insensitive to 1-MCP treatment. Collectively, these results showed that apple skin and pulp exhibited similar climacteric behaviour. The results also showed that the different parameters involved in ACC metabolism were differentially inhibited by the 1-MCP treatment during cold storage. ACS was completely inhibited in both tissues, ACO only partially and the treatment was ineffective to prevent MACC accumulation.  相似文献   

5.
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6.
Metabolic stress disinfection and disinfestation (MSDD) is a potential quarantine treatment in which a combination of cycles of rapid decompression and compression are followed by exposure to ethanol vapour under decompression. The response of ‘Hass’ avocado (Persea americana Mill., cv. Hass) to MSDD treatment for control of longtailed mealybug (Pseudococcus longispinus) was investigated. The best treatment for the most resistant life stage (2nd/3rd instars) was 90-min MSDD treatment with 371 mg L−1 ethanol. Early and late season ‘Hass’ avocados were subjected to MSDD treatments (with 371 mg L−1 ethanol), or in air (control). Following the treatments, early season fruit were ripened at 20 °C and 25 °C. Half of the late season fruit were ripened at either 20 °C or 25 °C, and the remainder were stored at 5.5 °C for 6 weeks, then ripened at 20 °C. There were no significant difference in quality and rot incidence between non-treated controls and MSDD-treated fruit. The main disorders found were stem-end and body rots, vascular browning and flesh greying for the stored fruit. There were also no significant differences in fruit respiration rate or ethylene production. Thus, MSDD was shown to be a potentially ‘soft’ disinfestation treatment for surface pests of avocado.  相似文献   

7.
To investigate the effects of postharvest application of 1-MCP on ethylene production and fruit softening, activities of ethylene biosynthesis and fruit softening enzymes were measured during postharvest ripening of plum (Prunus salicina Lindl. cv. Tegan Blue) fruit after being exposed to 1-MCP (0, 0.5, 1.0 or 2.0 μL L−1) at 20 ± 1 °C for 24 h. Following the treatments, fruit were allowed to ripen at ambient temperature (20 ± 1 °C), and ethylene production in fruit, activities of ACS and ACO, ACC content and fruit softening enzymes (PE, EGase, exo-PG and endo-PG) in fruit skin and pulp were recorded at different intervals. Postharvest application of 1-MCP significantly delayed and suppressed the climacteric ethylene production with reduction in the activities of ethylene biosynthesis enzymes (ACS, ACO) and ACC content, and fruit softening enzymes (PE, EGase, exo-PG and endo-PG) in the skin as well as in pulp tissues. The reduction was more pronounced with increased concentrations of 1-MCP. 1-MCP treated fruit showed different rates of fruit softening and activities of ethylene biosynthesis enzymes in the skin and pulp tissues which warrant further investigation on regulation of gene expression related to these enzymes with the inhibitory effect of 1-MCP.  相似文献   

8.
Fruit of cv. Monthong durian (Durio zibethinus) were treated with 0 (control) or 500 nL L−1 1-MCP for 12 h at 25 °C. Fruit were then stored at 15 °C. To determine storage life, every 3 days a batch of fruit was transferred to 25 °C. The time to ripeness (adequate eating quality) at 25 °C in controls (no 1-MCP) decreased from 5 days in freshly harvested fruit to 3 days after 18 days of storage at 15 °C. Storage life was considered adequate if the time to ripeness was ≥3 days. The storage life at 15 °C of control fruit (no 1-MCP) was therefore 18 days. After the 1-MCP treatment the time to ripeness at 25 °C was 7 days in fresh fruit, while in fruit stored at 15 °C for 30 days it was about 3 days. The storage life at 15 °C of 1-MCP-treated fruit was therefore 30 days. Pulp firmness and pulp total soluble solids (TSS) were determined after 3 day storage intervals at 15 °C and when the fruit was ripe at 25 °C. These parameters were only slightly affected by the 1-MCP treatment. Furthermore, 1-MCP had no effect on pulp color, but delayed yellowing of the fruit exterior. It is concluded that treatment with 1-MCP before storage at 15 °C extended storage life from 18 to 30 days.  相似文献   

9.
‘Empire’ apples [Malus sylvestris (L.) Mill var. domestica (Borkh.) Mansf.] are susceptible to development of chilling injury, expressed as firm flesh browning, during controlled atmosphere (CA) storage. Because of this susceptibility, fruit are typically stored at 2–4 °C, but the incidence of flesh browning can be increased by 1-methylcyclopropene (1-MCP) treatment at these temperatures. In this study, flesh browning development has been investigated in relationship to ethylene production, internal ethylene concentration (IEC), flesh firmness, total phenolic concentrations, and the activities of polyphenol oxidase (PPO) and peroxidase (POX) in the flesh tissues. Fruit were harvested from two orchards, either untreated or 1-MCP treated, and then stored under CA conditions at either 0.5 or 4 °C. Fruit were removed from storage at 1.5-month intervals for 10.5 months. 1-MCP treated apples were firmer than those of untreated apples, and had lower IECs, at all removals. Flesh browning incidence and severity developed earlier in 1-MCP-treated apples than untreated apples stored at either temperature. Total phenolic concentrations differed by orchard, but no major differences in concentrations were detected between untreated and 1-MCP treated apples. However, PPO activities were higher in the flesh of 1-MCP treated apples than untreated apples from both orchards and at both storage temperatures. POX activity was not consistently affected by 1-MCP treatment or storage temperature. Overall, our results suggest that inhibited ethylene production, either as a result of storage at 0.5 °C, or by treatment with 1-MCP at either temperature, may cause stress and damage to cells and result in higher PPO activity that leads to progressive flesh browning development during CA storage.  相似文献   

10.
Apricots are climacteric fruits with a high susceptibility to flesh softening and loss of flavor during postharvest storage, and most of the ripening processes are regulated by ethylene, which also has an effect on its own biosynthesis. To understand this process in apricot, inhibition of ethylene biosynthesis and perception was performed for studying key genes involved in the ethylene biosynthetic pathway. Apricots, cv. “Patterson”, were harvested with yellow-green ground color and immediately treated with either the ethylene perception inhibitor 1-methyl cyclopropene (1-MCP) at 10 μL L−1 or the ethylene biosynthesis inhibitor aminoethoxyvinylglycine (AVG) at 1 g L−1. After treatment, quality and physiological attributes such as firmness, color, total soluble solids, acidity, fruit weight, ethylene production and respiration rates were evaluated every 2 d until they ripened at 20 °C. Gene expression analysis was performed by quantitative polymerase chain reaction (qPCR). Both ethylene inhibitors were effective in reducing ethylene production, respiration rate and fruit softening. Three 1-aminocyclopropane-1-carboxylic-acid synthase (ACS) genes were characterized, but only the expression of ACS2 was highly reduced by ethylene inhibition, suggesting a key role in ethylene synthesis at ripening. Contrarily, ACS1 and ACS3 showed a higher expression under ethylene inhibition suggesting that the corresponding genes are individually regulated in a specific mode as observed in other climacteric fruits. Finally, changes in 1-aminocyclopropane-1-carboxylic-acid oxidase genes did not show a consistent pattern of ethylene modulation.  相似文献   

11.
The aim of this work was to study the specific effects of low temperature and 1-MCP treatment on ethylene metabolism and oxidative behaviour in plums (Prunus × salicina cv. Larry Ann). Control fruit were stored at 20 °C or 0 °C and the 1-MCP (625 nL L?1) treated fruit at 0 °C. Changes in the kinetics of ethylene production upon removal were related to changes in ACC metabolism (ACC and MACC levels), oxidative behaviour (H2O2 content) and enzymatic antioxidant potential (SOD, CAT and POX enzymes) during cold storage. Low temperature stress inhibited the synthesis of MACC, which appeared to be the basic process that regulated ACC and ethylene production at ambient temperature. Although 1-MCP treatment inhibited ethylene production and ACC accumulation in the cold, it did not inhibit the accumulation of MACC. Neither cold nor 1-MCP treatment induced oxidative stress. Nevertheless, the 1-MCP treatment significantly impaired the increase in POX activity observed during cold storage. Collectively these results showed the underlying role that ACC metabolism plays in the ripening behaviour of cold-stored plums, confirming previous results. The results also indicate that MACC and malonyl transferase activity are the key regulatory factors that control ripening and possibly some ethylene-related disorders such as chilling injury in cold-stored plums.  相似文献   

12.
‘Canino’ apricots and ‘Royal Zee’ plums were treated with 1000 nl l−1 1-methylcyclopropene (1-MCP) at 20 °C for 20 h following harvest before 0 °C storage. After 5 days storage for apricots and 10 days for plums and after 30 days storage for both, fruit were moved to 20 °C for ripening. In addition, apricots were stored for 20 days and then treated with 1-MCP concentrations of 10, 100 and 1000 nl l−1 at removal and held for ripening. Ethylene production and respiration rate, as well as fruit quality of apricots varied with treatment. Ethylene production was efficiently inhibited by 1000 nl l−1 1-MCP in fruit treated after storage but not in fruit treated before storage. Fruit softening was associated with ethylene production and affected by 1-MCP in a concentration dependent manner when treated after storage, while 1-MCP did not affect softening in prestorage treated fruit. The color change of fruit was ethylene-independent and not affected by 1-MCP. Internal flesh browning was decreased by 1-MCP regardless of the concentration when treated after storage, while it was enhanced in fruit treated before storage. Decay development in apricots was decreased by 1-MCP in a concentration dependent manner. Ethylene production and respiration in ‘Royal Zee’ plums was greatly inhibited by 1-MCP during ripening after both short-term (10 day) and long term (30 day) storage. Parameters associated with ripening processes were decreased significantly by 1-MCP, including softening, color change, and loss of titratable acidity. These data demonstrate that 1-MCP has potential to delay ripening of apricots and plums, but the cultivar, maturity of fruit, and time of application must be chosen carefully. It is suggested that 1-MCP is more efficient for extending the shelf life and improving the quality of ‘Canino’ apricots directly marketed or after storage, whereas it might be a potent compound for extending both storage period and shelf life of ‘Royal Zee’ plums.  相似文献   

13.
In mango (Mangifera indica) cv. Nam Dok Mai fruit, stored at 4 °C, peel browning occurred within 9 d, while no browning was found in cv. Choke Anan fruit stored at 4 °C for 30 d. During 6 d of shelf life at 27-28 °C, following various periods of low temperature storage, the peel browning in cv. Nam Dok Mai (if not yet maximal) became worse, whereas little browning was observed in cv. Choke Anan fruit. The pulp of the fruit of both cultivars did not show browning during the 4 °C storage, but the pulp of cv. Nam Dok Mai exhibited some browning during shelf life if the fruit had been stored at 4 °C for more than 18 d. Peel and pulp color were not correlated with total free phenolics. A high correlation coefficient was observed between peel browning and PAL activity in the peel, while a very low correlation was found with peel catechol oxidase activity. The browning in the pulp was not correlated with the measured enzyme activities. The data therefore show a relation between PAL activity in the peel and low temperature-induced peel browning.  相似文献   

14.
The combined use of preharvest treatments, gibberellic acid (GA3) or calcium nitrate, with 1-methylcyclopropene (1-MCP) treatment applied postharvest, was evaluated to improve the storability of ‘Rojo Brillante’ persimmon fruit, at both 1 and 15 °C. Properties linked to commercial quality, such as flesh firmness, external colour, total soluble solids and level of astringency, were evaluated at harvest, periodically during storage, as well as after subsequent shelf-life periods. At both storage temperatures, control fruit and calcium nitrate-treated fruit showed commercial quality for 20 d; the sole application of GA3 delayed loss of firmness for 30 d while the treatment with 1-MCP by itself allowed storage of the fruit for 40 d. The combined use of calcium nitrate plus 1-MCP did not improve maintenance of quality any more than when 1-MCP was applied alone. The combination of GA3 and 1-MCP delayed the symptoms of chilling injury, extending the storability at 1 °C for up to nearly 3 months. During storage at 15 °C, the combination of both treatments resulted in high-firmness values for 30 d, but did not prolong the storage period any longer than the 40 d reached by the sole application of 1-MCP. Irrespective of treatment, a loss of efficacy of the deastringency treatment was observed after 30 d of storage at 15 °C.  相似文献   

15.
The potential of 1-MCP for controlling ripening in ‘Angeleno’ plum fruit under air and controlled atmosphere (CA) storage was explored, and the possibility that 1-MCP can inhibit development of brown rot caused by Monilinia laxa and internal breakdown in ‘Fortune’ and ‘Angeleno’ plums tested. After harvest, fruit were exposed to 300 and 500 nl l−1 (in 2003) and 500 nl l−1 1-MCP (in 2004) at low temperatures (0–3 °C) for 24 h. After treatment the plums were stored in air at 0 °C and ‘Angeleno’ fruit were also stored in CA storage (1.8% O2 + 2.5% CO2). Following storage, fruit were kept at 20 °C. In ‘Angeleno’ fruit, 1-MCP was effective in delaying the loss of firmness and colour changes during holding at 20 °C. 1-MCP reduced brown rot in fruit stored in CA but no significant reduction was found in air storage. Internal breakdown, a major physiological storage disorder in plums, was inhibited by 1-MCP treatment. Furthermore, since 1-MCP applied in air storage showed better results than the control in CA conditions, an application of 1-MCP before air storage could be the best way to reduce the ripening process for short or medium storage periods (40 and 60 days). CA storage plus 1-MCP treatment could be used for long periods (80 days).  相似文献   

16.
Quality changes of apple fruit at different maturity stages in response to heat stress were investigated. ‘Jonagold’ and ‘Cortland’ apples at immature (pre-climacteric), commercial harvest maturity (CHM) and post climacteric maturity (PCM, CHM plus 4 weeks) were harvested and held at 46 °C for 0, 4, 8, or 12 h. Following treatments, fruits were stored in air at 0 °C and evaluated after 0, 1, 2, or 3 months. Quality indices including peel and flesh browning, firmness, titratable acidity, soluble solids, chlorophyll fluorescence (CF), and ethanol production were measured. Results indicated that different cultivars and maturities affected the fruit's resistance to heat stress. ‘Jonagold’ was more resistant to heat stress than ‘Cortland’. Fruit at PCM were most sensitive to heat stress, followed by fruits at CHM and immature stages. When ‘Jonagold’ apples at immature and CHM stages were held at 46 °C for 12 h and then stored for 3 months, flesh browning ratings were negligible compared with 1.4 or 2.9, respectively in ‘Cortland’. Flesh browning rating increased to 1.4 or 4.5 in PCM ‘Jonagold’ held at 46 °C for 8 or 12 h and then stored for 3 months while it was 4.9 or 5.0, respectively, in ‘Cortland’. Heat treatment-induced flesh injury was associated with a decrease in CF. After fruit were exposure to 46 °C for 12 h and then stored for 3 months, Fv/Fm was reduced by 13%, 30%, and 55% in ‘Jonagold’ at immature maturity, CHM and PCM, respectively, while it was reduced by 51%, 58% and 75%, respectively, in ‘Cortland’. Heat stress also caused a decrease in fruit titratable acidity, but had no effect on soluble solids contents. The 8 or 12 h heat treatment resulted in an increase in ethanol production, which was greatest in PCM apples.  相似文献   

17.
Longkong fruit abscission was found to be sensitive to external ethylene at concentrations as low as 0.05 μL L−1. Ethylene induced fruit drop at the junction between the main peduncle and the calyx, at a clear abscission zone. Fruit drop at the junction between calyx and the fruit, however depended on an external force. There may or may not be an abscission zone at this site. Dipping longkong fruit in 200 μL L−1 NAA solution delayed fruit abscission and slightly reduced ethylene production. NAA application was also found to reduce the effect of external ethylene treatment. Applications of 1 μL L−1 1-MCP for 6 h minimized the effect of external ethylene and almost doubled storage life of longkong. The combination of NAA and 1-MCP treatment did not give a synergistic effect.  相似文献   

18.
Anaerobic metabolism during short and long term storage of kiwifruit   总被引：1，自引：0，他引：1  
Kiwifruit were stored in 0.25% O2 (ULO), 1% O2 (LO) and 2% O2 + 5% CO2 (CA) and the controls were kept in air. The fruit were held at 0 °C for 34 and 94 d of storage and, after these times, were transferred to 20 °C in air for 14 d of shelf life. During the short term storage (34 d), a significant increase in anaerobic metabolites, above all ethanol, was observed in ULO, LO, and CA fruit (166, 131, 120 μL/L). After the shift to shelf life, a large and unexpected increase in PDC (pyruvate decarboxylase), ADH (alcohol dehydrogenase in the direction of ethanol oxidation), LDH (lactate dehydrogenase), and GPT (glutamate-pyruvate transaminase) was observed, resulting in ethanol depletion (ULO) or no further increase, and an increase in acetaldehyde which, in turn, could have hastened fruit ripening. Even the control fruit showed an increase in ethanol during storage and an increase in enzyme activity during shelf life, especially in ADH, but to a lesser extent without an increase in acetaldehyde. During the long term storage, anaerobic metabolites (ethanol and acetaldehyde) still increased and GPT activity rose significantly in the ULO and CA samples. A burst of enzyme activity was also observed during the second shelf life in the CA and LO samples, but not in the control, while in ULO fruit the activity rose continuously. GPT activity showed the highest peaks in CA and ULO fruit. An ethylene burst was observed in ULO and CA fruit during the second shelf life (about 25 μL/kg-h) but not during the first shelf life. The potential role of these enzymes in kiwifruit stress response during storage and shelf life is discussed.  相似文献   

19.
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20.
The objective of this study was to evaluate the use of an ethanol vapor release pad and a saprophytic yeast Cryptococcus infirmo-miniatum (CIM) to reduce decay and maintain postharvest quality of intact or fresh-cut sweet cherries (Prunus avium) cv. Lapins and Bing. Intact or fresh-cut fruit were packed in perforated clamshells (capacity 454 g) and stored at 1, 10 or 20 °C for up to 21, 14 and 8 d, respectively. For ethanol treatment, a pad made with silica gel powder containing 10 g ethanol and covered with perforated film, which allows ethanol vapor to diffuse gradually, was attached to the upper lid of the clamshells. Ethanol treatment caused accumulation of ethanol in the packaging headspace, about 10 μL L−1 with little change within 14 d at 1 °C, 23 μL L−1 at d 1 and decreased to 15 μL L−1 at d 10 at 10 °C, and 26 μL L−1 at d 1 and decreased to 13 μL L−1 at d 3 at 20 °C. Ethanol content in fruit was less than 9 mg kg−1 in all the control fruit, and increased to 16, 34 and 43 mg kg−1 in ethanol-treated fruit at 1, 10 and 20 °C, respectively. Nonetheless, a sensory taste panel did not perceive any flavor difference from the ethanol treatment. The ethanol treatment retarded softening, darkening, and acid decrease in fruit as well as discoloration of the stems, and extended shelf-life of intact cherries. Ethanol reduced brown rot (Monilinia fructicola) in fresh-cut cherries stored at 20 °C, but not at 1 and 10 °C. A pre-packaging dip in CIM completely controlled brown rot in inoculated fresh-cut cherries stored at 1 °C, and in naturally infected cherries at 20 °C.  相似文献   

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