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Down-Regulated Expression of RACK1 Gene by RNA Interference Enhances Drought Tolerance in Rice 总被引：2，自引：0，他引：2
The receptor for activated C-kinase 1 (RACK1) is a highly conserved scaffold protein with versatile functions, and plays important roles in the regulation of plant growth and development. Transgenic rice plants, in which the expression of RACK1 gene was inhibited by RNA interference (RNAi), were studied to elucidate the possible functions of RACK1 in responses to drought stress in rice. Real-time PCR analysis showed that the expression of RACK1 in transgenic rice plants was inhibited by more than 50%. The tolerance to drought stress of the transgenic rice plants was higher as compared with the non-transgenic rice plants. The peroxidation of membrane and the production of malondialdehyde were significantly lower, and the superoxide dismutase activity in transgenic rice plants was significantly higher than those in non-trangenic rice plants. It is suggested that RACK1 negatively regulated the redox system-related tolerance to drought stress of rice plants. 相似文献
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Abiotic stress confers serious damage to the photosynthetic machinery, often resulting in plant growth inhibition. Hypothetical chloroplast open reading frame 3 (Ycf3)-interacting protein 1 (Y3IP1) is a nucleus-encoded thylakoid protein and plays an essential role in the assembly of photosystem I. The full-length cDNA over-expresser (FOX) gene-hunting system is an approach using systemically generated gain-of-function mutants. Among the FOX-rice lines, a line CE175 overexpressing rice Y3IP1 gene (OsY3IP1) displayed less inhibition of root growth under saline (NaCl) stress. The expression of OsY3IP1 was up-regulated under saline and alkaline (Na2CO3) stresses in the rice variety Kitaake. After saline and alkaline treatments, transgenic Kitaake overexpressing OsY3IP1-GFP (OsY3IP1-GFPox/Kit) displayed higher levels of chlorophyll content compared to Kitaake. Under the stress conditions, the maximum quantum yield of photosystem II photochemistry levels was higher in OsY3IP1-GFPox/Kit than in Kitaake. The increased tolerance conferred by OsY3IP1 overexpression correlated with reduced reactive oxygen species accumulation. Our data provide new insights into the possible role of OsY3IP1 in the pathway suppressing photooxidative damage under stress conditions. These features can be further exploited to improve saline and alkaline tolerances of rice plants in future. 相似文献
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RNA干涉下调RACK1基因表达增强水稻抗旱能力 总被引：1，自引：0，他引：1
RACK1是一种多功能支架蛋白,广泛参与植物生长发育过程的调节。利用RNA干涉技术抑制水稻RACK1基因的表达,分析了RACK1基因在响应干旱胁迫中的功能。实时定量PCR对获得的转基因植株的RACK1基因表达分析结果表明,转基因水稻RACK1基因表达受抑制程度达50%左右。与非转基因水稻（对照）相比,转基因水稻耐干旱能力显著强于对照,其膜的过氧化酶和丙二醛的产生显著低于对照,而超氧化物歧化酶活性极显著高于对照。表明RACK1蛋白质调节水稻对干旱胁迫的耐性,并且这种调节在很大程度上与植株体内的氧化还原系统有关。 相似文献
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Dan Zeng Chunchao Wang Junpin Xie Fan Zhang Jialing Lu Xiaorong Shi Yingyao Shi Yongli Zhou 《水稻科学》2021,28(6):547-556
Soil salinity is an environmental threat limiting rice productivity. Identification of salinity tolerance genes and exploitation of their mechanisms in plants are vital for crop breeding. In this study, the function of stress-activated protein kinase 7(OsSAPK7), a SnRK2 family member, was characterized in response to salt stress in rice. Compared with variety 9804, OsSAPK7-overexpression plants had a greater survival rate, increased chlorophyll and proline contents, and superoxide dismutase and catalase activities at the seedling stage under salt-stress conditions, as well as decreased sodium potassium ratio(Na~+/K~+) and malondialdehyde contents. After salt stress, the OsS APK7 knockout plants had lower survival rates, increased Na~+/K~+ ratios and malomdiadehyde contents, and decreased physiological parameters compared with 9804. These changes in transgenic lines suggested that OsSAPK7 increased the salt tolerance of rice by modulating ion homeostasis, redox reactions and photosynthesis. The results of RNA-Seq indicated that genes involved in redox-dependent signaling pathway, photosynthesis and zeatin synthesis pathways were significantly down-regulated in the OsSAPK7 knockout line compared with 9804 under salt-stress condition, which confirmed that OsSAPK7 positively regulated salt tolerance by modulating diverse stress-defensive responses in rice. These findings provided novel insights for the genetic improvement of rice and for understanding the regulatory mechanisms of salt-stress tolerance. 相似文献
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为明确茶树[Camellia sinensis(L.)O.Kuntze.]谷胱甘肽过氧化物酶(Glutathione peroxidases,GPX)编码基因CsGPX1的功能,本文利用茶树转录组数据克隆获得CsGPX1的编码区全长序列,进行序列比对与分析,在此基础上,将CsGPX1在烟草中进行过表达获得转CsGPX1基因烟草,并比较野生型烟草与转基因烟草耐旱性的差异,验证CsGPX1功能。序列分析表明,CsGPX1编码序列(CDS)长723 bp,编码240个氨基酸。BLAST比对发现,该基因与桃树(Prunus persica)的GPX基因具有高度同源性(>85%)。CsGPX1编码的氨基酸序列具有GPX蛋白保守特征序列和区别于其他家族成员的特有的结构域——磷脂氢谷胱甘肽过氧化物酶(PHGPX)。干旱胁迫处理结果显示,过表达CsGPX1烟草株系抗旱性强于野生型。GPX酶活性测定结果表明,转基因植株的GPX酶活性高于野生型植株。以上研究结果表明,过表达CsGPX1可提高转基因烟草的耐旱能力,说明CsGPX1可能与茶树的耐旱性能相关。本研究为提高茶树的耐旱性能研究提供了新的理论途径,并对降低茶园管理的成本具有一定的积极意义。 相似文献
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NAC转录因子在植物非生物逆境胁迫应答中发挥重要作用,研究芒果MiNAC1基因的功能为芒果的抗逆性育种提供基因资源。干旱、盐和低温等非生物胁迫严重影响芒果的生长发育。前期研究中,课题组从芒果逆境胁迫转录组中获得了一个MiNAC1基因,表达模式分析发现其与芒果的逆境胁迫应答有关。本研究对芒果MiNAC1基因的功能进行了验证。将芒果MiNAC1基因构建到pBI121-MiNAC1超量表达载体中,并利用农杆菌介导的花序浸染法转化野生型拟南芥,对获得的T3代纯合株系进行表型观察分析和逆境胁迫处理。结果显示,转芒果MiNAC1基因与野生型拟南芥的表型类似,转基因不影响拟南芥的莲座叶数量、抽薹时间、开花时间以及开花时的植株高度。逆境胁迫处理：分别用0、200、300、400 mmol/L甘露醇进行干旱胁迫;用0、100、150、200 mmol/L NaCl进行盐胁迫;4℃低温胁迫处理转基因与对照拟南芥植株。结果显示：随着甘露醇处理浓度的增加,转基因株系与对照组拟南芥的根系生长发育均受到抑制,但转基因株系受到抑制的影响显著小于对照组拟南芥,比如在300 mmol/L甘露醇处理时,转基因株系的根长显著增长,OE9的根长是WT的1.51倍,侧根数量显著增加,OE7的侧根数是WT的5倍,另外根冠比分析显示,转基因植株的根冠比显著高于对照植株,OE2的根冠比是WT的1.53倍。盐胁迫和低温胁迫也取得了类似的结果。以上结果表明转芒果MiNAC1基因可以通过增加转基因植株的根长和侧根数量提高其对干旱胁迫、盐胁迫和低温胁迫的抗性。本研究初步揭示了MiNAC1基因的功能,为深入研究MiNAC1基因参与调控芒果逆境胁迫调控网络奠定基础。 相似文献
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OsBTF3过量表达和RNAi转基因水稻抗盐和抗低温胁迫鉴定 总被引：2，自引：0，他引：2
为评估水稻OsBTF3基因在水稻对非生物逆境胁迫应答中的功能,利用RT Q PCR技术,对经高盐(200 mmol/L NaCl溶液)和低温(4℃)胁迫处理后水稻的OsBTF3基因表达动态进行了测定,并对前期获得的OsBTF3过量表达和RNAi转基因水稻株系进行了抗高盐和抗低温胁迫的鉴定。结果表明,高盐胁迫处理显著抑制了OsBTF3基因的表达,低温胁迫对其表达影响有一定的变化;转基因T2代过量表达株系对高盐和低温胁迫抗性显著增强,而RNAi转基因株系抗性减弱,说明OsBTF3可能在水稻对高盐和低温胁迫反应中起着重要的调控作用。 相似文献
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Background
The SUV3 (suppressor of Var 3) gene encodes a DNA and RNA helicase, which is localized in the mitochondria. Plant SUV3 has not yet been characterized in detail. However, the Arabidopsis ortholog of SUV3 (AT4G14790) has been shown to be involved in embryo sac development. Previously, we have reported that rice SUV3 functions as DNA and RNA helicase and provides salinity stress tolerance by maintaining photosynthesis and antioxidant machinery. Here, we report further analysis of the transgenic OsSUV3 rice plants under salt stress.Findings
The transgenic OsSUV3 overexpressing rice T1 lines showed significantly higher endogenous content of plant hormones viz., gibberellic acid (GA3), zeatin (Z) and indole-3-acetic acid (IAA) in leaf, stem and root as compared to wild-type (WT), vector control (VC) and antisense (AS) plants under salt (200 mM NaCl) stress condition. A similar trend of endogenous plant hormones profile was also reflected in the T2 generation of OsSUV3 transgenic rice under defined parameters and stress condition.Conclusions
In response to stress, OsSUV3 rice plants maintained plant hormone levels that regulate the expression of several stress-induced genes and reduce adverse effects of salt on plant growth and development and therefore sustains crop productivity. 相似文献11.
Small ubiquitin-like modifier (SUMO)-conjugating enzymes are involved in post-translational regulatory processes in eukaryotes, including the conjugation of SUMO peptides to protein substrate (SUMOylation). SUMOylation plays an important role in improving plant tolerance to abiotic stress such as salt, drought, heat and cold. Herein, we reported the isolation of OsSCE1 (LOC_Os10g39120) gene encoding a SUMO-conjugating enzyme from rice (Oryza sativa cv. Nipponbare) and its functional validation in response to drought stress. The E2 enzyme, OsSCE1, is one of three key enzymes involved in the conjugation of SUMO to its target proteins. Activated SUMO is transferred to the cysteine of an E2 enzyme and then to the target lysine residue of the substrate, with or without the help of an E3 SUMO ligase. Expression of OsSCE1 was strongly induced by polyethylene glycol 6000 (PEG6000) treatment, which suggested OsSCE1 may be involved in the drought stress response. Overexpression of OsSCE1 (OsSCE1-OX) in Nipponbare reduced the tolerance to drought stress. Conversely, the drought tolerance was slightly improved by the knockdown of OsSCE1 (OsSCE1-KD). These results were further supported by measurement of proline content in OsSCE1-OX and OsSCE1-KD transgenic lines under induced drought stress, which showed OsSCE1-KD transgenic lines accumulated higher proline content than the wild type, whereas OsSCE1-OX line had lower proline content than the wild type. These findings suggested OsSCE1 may play a role as a negative regulator in response to drought stress in rice. 相似文献
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The homozygous T3 transgenic lines with sense OsCBL8 gene and antisense OsCBL8 gene obtained by agro-transformation were used to investigate the function of OsCBL8 in rice. Semi-quantitative RT-PCR showed that the expression of OsCBL8 extremely increased in sense transgenic lines, and decreased to some extents in antisense transgenic lines. Such up- and down-regulation of the OsCBL8 gene in these transgenic lines had little effects on main agronomic traits, but significantly decreased the number of filled grains per panicle and seed setting rate in some of transgenic lines. By evaluation of the tolerance to 150 mmol/L NaCl, 20% PEG6000 and low temperature treatments, and relevant physiological indices, 8F12, a sense transgenic line with high salt tolerance, and 8R14, an antisense transgenic line with high drought tolerance, were obtained, which suggests that the OsCBL8 gene is involved in the response of rice to abiotic stresses. 相似文献
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Lipoxygenase 3 (LOX3) is a major component of the LOX isozymes in mature rice seeds. To investigate the role of LOX3 gene under stresses, a plant expression vector containing antisense cDNA of LOX3 was constructed. Rice varieties Wuyunjing 7 and Kasalath were transformed by the Agrobacterium-mediated method and transgenic rice plants were generated. PCR and Southern blot results showed that the antisense LOX3 gene was integrated into the rice genome. Analyses of embryo LOX3 deletion and semi-quantitative RT-PCR confirmed the antisense suppression of LOX3 gene in transgenic plants. The T2 antisense plants of LOX3 were sensitive to drought stress, rice blast and bacterial blight compared with non-transgenic plants. These results suggest that the LOX3 gene might function in response to stresses. 相似文献
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为了解玉米C_4型光合酶基因对C3植物拟南芥光合特性的影响及其对干旱胁迫的响应,分别以过表达ZmPEPC(磷酸烯醇式丙酮酸羧化酶)、ZmPPDK(丙酮酸磷酸二激酶)和ZmNADP-ME(依赖于NADP的苹果酸酶)单个酶基因的拟南芥株系(分别简写为PC、PK、ME),以及过表达PEPC+PPDK和PPDK+NADP-ME两个酶基因的拟南芥株系(分别简写为PCK、PKM)为供试材料,在开花期停止浇水,并分别于干旱胁迫处理前1天、第5天、第10天和结束干旱胁迫复水处理5d时测定转基因拟南芥中目标基因的表达量、光合速率、水分利用效率和酶活性。结果表明,在正常生长条件下,PCK类型株系的PEPC酶活性、PPDK酶活性、净光合速率(Pn)以及水分利用效率(WUE)较野生型拟南芥分别高52%、20%、24%和55%,除PPDK酶活性外,PCK类型株系各指标测定值的增幅均高于其他转基因类型株系,综合表现最优。不同类型株系的上述测定指标总体表现为PCKPCPK、PKMME。干旱胁迫处理5d时,各类型株系中目标基因的表达量、光合酶活性、Pn和WUE均有所上升。干旱胁迫处理10d时,拟南芥受到严重损伤,上述指标的测定值均下降。复水5d时上述指标得到不同程度的恢复,不同类型转基因株系的各项测定指标在不同干旱胁迫处理中总体仍表现为PCK最优,PC次之。各转基因株系均优于受体非转基因拟南芥。 相似文献
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《Field Crops Research》2006,97(1):66-76
Low and unstable rice productivity in many areas of Asia is associated with many abiotic and biotic stresses such as drought, salinity, anaerobic conditions during germination, submergence, phosphorus and zinc deficiency, etc. To develop rice varieties with tolerance to these stresses, we undertook a large backcross (BC) breeding effort for the last 6 years, using three recurrent elite rice lines and 203 diverse donors, which represent a significant portion of the genetic diversity in the primary gene pool of rice. Significant progress has been made in the BC breeding program, which resulted in development of large numbers of introgression lines with improved tolerance to these stresses. Promising lines have been developed with excellent tolerances (extreme phenotypes) to salinity, submergence and zinc deficiency; resistance to brown plant hopper, ability to germinate under the anaerobic condition and low temperature. Our results indicated that there exist tremendous amounts of ‘hidden’ diversity for abiotic and biotic stress tolerances in the primary gene pool of rice. Furthermore, we demonstrated that despite the complex genetics and diverse physiological mechanisms underlying the abiotic stress tolerances, introgression of genes from a diverse source of donors into elite genetic backgrounds through BC breeding and efficient selection (careful screening under severe stress) is a powerful way to exploit this hidden diversity for improving abiotic stress tolerances of rice. We have developed three large sets of introgression lines, which not only provide an unique platform of breeding materials for developing new rice cultivars with superior yield and stability by trait/gene pyramiding, but also represent unique genetic stocks for a large-scale discovery of genes/alleles underlying the abiotic and biotic stress tolerances of rice using genomic tools. 相似文献
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植物低磷与低温胁迫应答之间存在基因调控及生理生化适应关联性。MYB-CC家族转录因子PHR1是植物低磷应答核心调控因子,但PHR1是否参与植物低温胁迫应答调控及其扮演的功能还不清楚。本文以BnPHR1过量表达转基因油菜为材料,探究BnPHR1在油菜低温胁迫应答中的调控功能。相比野生型油菜,BnPHR1过量表达转基因油菜株系对低温的耐受性明显提高,叶片萎焉程度减轻。电导率和丙二醛含量分析表明转基因油菜细胞膜损伤程度降低。为了进一步探究BnPHR1在低温胁迫中的作用,从野生型（WT）及BnPHR1过量表达转基因油菜转录组数据中挖掘低温胁迫应答相关差异表达基因,探索BnPHR1影响油菜低温抗性的可能机制。转录组数据分析结果显示,在油菜地上部分和根BnPHR1调控的差异基因中,分别有44和49个低温应答相关基因,如BnCOR15B,BnCOR78,BnCBF2等。BnPHR1调控差异基因启动子分析发现26个差异基因启动子中含有P1BS元件,其可能受BnPHR1直接调控。这些结果表明BnPHR1可能通过影响下游低温应答相关基因表达提高转基因油菜应对低温胁迫耐受性。 相似文献