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1.
为了充分利用优良母牛遗传资源,发挥超数排卵技术对改良奶牛品种的重要作用,采用CUE-MATE孕酮栓+FSH+PGF2α法和自然发情+FSH+PGF2α法对酒泉地区饲养的奶牛进行了超数排卵研究。结果表明:自然发情法和CUE-MATE法超排回收的平均可用胚胎数分别为4.54枚和4.86枚;上、中、下3等膘情供体牛获得的平均可用胚胎数分别为6.13、5.39和3.29枚,可用胚率分别为58.60%、58.08%和44.66%;采用CUE-MATE孕酮栓+FSH+PGF2α方法进行超排处理,平均可用胚胎数:春季(4.00枚)≤秋季(4.86枚)≤冬季(5.07枚),平均可用胚率:秋季(56.04%)>冬季(50.71%)>春季(43.50%);青年母牛的超排效果略高于经产母牛,但两者间差异不显著(P>0.05)。 相似文献
2.
通过在酒泉地区应用推广超数排卵技术,探讨当地供体母牛的选择基本条件及最佳超排季节。采用自然发情+FSH+PGF2α法和CUEMAT孕酮栓+FSH+PGF2α法,研究了不同激素处理、季节、供体母牛体况、胎次等因素对超数排卵效果的影响。结果表明:自然发情法和CUE-MATE法超排的平均可用胚胎数分别为4.54枚和4.86枚,上、中、下3等膘情获得的平均可用胚胎数分别为6.13枚、5.39枚和3.29枚,平均可用胚数春季(4.00枚)<秋季(4.86枚)<冬季(5.07枚),经产母牛和青年母牛平均可用胚数分别为9.06枚和9.65枚。由此可见,酒泉地区供体母牛可选取膘情体况在中等以上,26胎经产母牛或1.56胎经产母牛或1.52.0岁未产青年母牛为宜,时间在秋、冬两季节进行超数排卵较为理想。 相似文献
3.
超数排卵母牛血浆孕酮、雌二醇和促黄体素含量与卵巢反应及胚胎质量的关系 总被引：2，自引：0，他引：2
对9头FSH超排母牛血浆孕酮,雌二醇和促黄体素水平进行了研究。超排第1天到发情后第4天的血浆孕酮含量与排卵数的相关不显著,发情后第7天的孕酮含量与排卵数的相关极显著(r=0.849.P<0.01);发情前的雌二醇含量与排卵数有弱的正相关,发情后第7天的雌二醇含量与排卵数有极显著的负相关(r=-0.809,P<0.01);促黄体素的含量与排卵数无关。另将供体母牛随机分为A、B两组,A组注射LRH—A_3,B组不注射,作对照。结果发现,两组间排卵数、采卵数,可用卵数和未排大卵泡数的差异在统计上均不显著。本试验结果表明,超排前血浆孕酮含量不适宜预测卵巢反应,而发情后第7天的孕酮含量是评定卵巢反应的一项合适的指标。超排时给予LRH-A_3的效果虽不十分显著,但有正效果,值得进一步研究。 相似文献
4.
在发情周期第10天,对59头婆莫云(BMY)供体牛进行4 d递减剂量的FSH-P超数排卵试验,结果表明:①在超排前3 d(发情周期第7天)卵巢有卵泡(直径≥5 mm)+黄体的牛超排头均可用胚数3.71枚,比只有黄体的牛高2.11枚,差异显著(P<0.05)。②第1次重复超排头均可用胚高达5.57枚,分别比第2、3、4次重复超排高(P>0.05)2.15、3.29、3.15枚,比随机选择供体的超排1组头均可用胚多4.57枚,差异显著(P<0.05)。试验结果提示,超排处理前3 d(周期第7天)复选供体牛时,没有必要淘汰那些卵巢上有黄体但也有卵泡的供体牛;选出超排效果好的母牛进行重复超排是提高超排效果的有效措施。 相似文献
5.
红安格斯牛超数排卵和胚胎移植受体同期发情处理 总被引：3，自引：0，他引：3
选择100头红安格斯母牛使用2种超数排卵处理方案,有效超排供体母牛77头。对受体牛采用3种方案进行同期发情处理。结果表明:育成母牛和经产母牛平均回收胚胎数分别为8.45、9.23枚(P>0.05),平均回收可用胚胎数分别为6.33、7.02枚(P>0.05);2种超排方案均取得较好的效果,超排方案1和方案2分别回收胚胎数为10.10、7.63枚,回收可用胚胎数为6.62、6.51枚。受体同期发情处理一次PG法、二次PG法及CIDR+PG法,同期发情率分别为71.19%、74.44%和77.63%(P>0.05),发情受体利用率分别为66.67%、64.93%及69.49%(P>0.05),胚胎移植妊娠率分别为50.00%、55.17%和56.10%(P>0.05),与自然发情受体利用率及胚胎移植妊娠率无显著性差异。 相似文献
6.
在供体奶牛超排时,应用X性控冷冻精液进行输精,生产体内性控胚胎.超排处理72头供体母牛(每头输4支精液),获可用胚335枚,平均每头供体牛获可用胚4.65枚.对照组共处理供体牛68头,采用常规冷冻精液输精(每头牛输精2支),获可用胚415枚,头均获可用胚6.1枚. 相似文献
7.
泌乳期波尔山羊的重复超排及胚胎移植效果分析 总被引：1，自引：0，他引：1
对26只泌乳期波尔山羊实施2次重复超排处理。其结果如下:①第一次超排有效率为84.62%,平均排卵数为19.5±6.42(枚/只),平均采集胚胎数为15.86±1.84(枚/只),平均采集可用胚胎数为10.5±8.32(枚/只)。第二次重复超排处理时有1只羊推迟发情,超排有效率为96.15%,平均排卵数为28.36±5.26(枚/只),平均采集胚胎数21.48±7.42(枚/只),平均采集可用胚胎数为18.64±4.12(枚/只);②两次重复超排所获得的可用胚胎数差异极显著(P<0.01)。泌乳期供体羊第一次采集的可用胚胎数量[10.50±8.32(枚/只)]显著低于断奶后供体羊[18.64±4.12(枚/只)];③胚胎移植后的2组羊产羔率差异不显著(P>0.05)。 相似文献
8.
澳洲和牛超数排卵与体内胚胎生产的影响因素研究 总被引：1，自引：0，他引：1
旨在研究不同发育阶段（青年和经产牛）、同期方法、促卵泡素（follicle-stimulating hormone,FSH）剂量、超排次数等因素对澳洲和牛供体超数排卵（简称超排）和体内胚胎生产效率的影响。本试验共选用38头青年和牛（16~20月龄）和42头经产和牛（胎次=1）进行超排处理,分别统计每头供体牛回收的总胚胎数、可用胚胎数、不同等级胚胎数以及不可用胚胎数,然后分别根据不同发育阶段、同期方法、FSH剂量以及超排次数对各试验组的超排效率、胚胎生产等数据进行比较分析。结果表明：1）经产牛的超排有效率（（95.46±2.58）%）显著高于青年牛（（77.55±6.02）%）（P<0.05）,胚胎总数（12.30 vs 10.37枚）、可用胚胎数（8.97 vs 7.66枚）、A级胚胎数（3.86 vs 2.50枚）均高于青年牛,但组间差异不显著（P>0.05）;2）超排前不同同期处理方法（自然发情、PG和埋植CIDR）的超排有效率差异不显著,但是自然发情组供体牛头均可用胚胎总数（（11.22±2.33）枚）显著高于PG处理组（（6.09±1.05）枚）（P<0.05）;3）不同剂量FSH可显著影响供体的超排效率和头均不可用胚胎数,青年牛120 mg FSH组的超排有效率（（55.56±17.57）%）显著低于200 mg FSH （（88.00±6.63）%）和220 mg FSH组（（73.33±11.82）%）（P<0.05）,经产牛220 mg FSH组的头均不可用胚胎数（（2.28±0.65）枚）显著低于250 mg FSH组（（5.00±1.38）枚）（P<0.05）;4）超排次数显著影响青年牛超排有效率和经产牛头均不可用胚胎数,青年牛第一次超排有效率（（70.27±7.62）%）显著低于第二次超排有效率（100%）（P<0.05）,经产牛第二次超排回收的不可用胚胎数（（4.44±0.92）枚）显著高于第一次超排（（2.39±0.40）枚）（P<0.05）。因此,在华北地区现有的生产管理水平下,和牛超排供体宜选用经产母牛,超排处理FSH总剂量宜为240 mg,这对和牛体内胚胎高效生产和核心群的建立具有一定的参考价值。 相似文献
9.
研究主要针对CIDR放置时间长短对绵山羊超排效果的影响进行研究,以期为羊胚胎工程技术产业化提供坚实的基础.研究结果表明绵羊第10天起针的供体,平均回收数8.94枚±3.78枚,平均可用胚7.67枚±3.76枚,胚胎可用率90.34%.第13天起针超排的供体绵羊,平均回收8.38枚±3.79枚,平均可用胚6.37枚±3.03枚,胚胎可用率为76.03 %;第10天起针平均可用胚高于第13天起针的超排效果,两者差异显著(P＜0.05),两者之间平均回收胚基本一样,无显著差异(P＞0.05),但第10天起针的胚胎利用率高于第13天起针的胚胎可用率,差异极显著(P＜0.01).CIDR放置9天起针的供体山羊,平均可用胚为17.59枚±3.22枚,胚胎可用率87.40 %;CIDR放置15天起针的供体,平均可用胚数为12.73枚±4.01枚,胚胎可用率为67.95 %.CIDR放置9天起针平均可用胚高于CIDR放置15天起针的超排效果,差异极显著(P＜0.01),CIDR放置9天起针的胚胎可用率高于放置15天的胚胎可用率,差异极显著(P＜0.01).波尔山羊在11月、12月这种结果更明显;4月份虽然9天比15天高,但平均可用胚数和胚胎可用率之间没有差异(P＞0.05),而3月份平均可用胚差异显著(P＜0.05),胚胎可用率差异显著(P＜0.05). 相似文献
10.
GnRH类似物LRH—A3配合FSH对超排母牛胚胎质量的影响 总被引：5，自引：0，他引：5
对４５头供体母牛随机用ＦＳＨ＋ＬＲＨ－Ａ３和ＦＳＨ进行超排处理。其中ＦＳＨ＋ＬＲＨ－Ａ３处理供体５２头次，ＦＳＨ处理供体２２头次；ＦＳＨ＋ＬＲＨ－Ａ３头处理组平均获得卵数，可用胚胎数分别是１０．１±８．０枚，７．４±６．９枚，与ＦＳＨ处理组（二指标分别是９．３±６．２枚，３．６±２．７）相比，分别高８．６％，１０５．６％，且二组超排获得可用胚胎数差异显著（Ｐ＜０．０５）。 相似文献
11.
本实验采用放射免疫测定技术(RIA)分析了9头屡配不孕母牛和6头正常母牛于发情开始后8天内的血清雌二酵和孕酮含量的变化。测定结果表明:屡配不孕母牛发情开始时的血清雌二醇水平低于正常母牛(7.4±3.4pg/ml VS 14.2±6.3pg/ml p<0.025)。发情开后96小时内屡配不孕母牛的血清孕酮水平的变化范围为0.90～1.76ng/ml,正常母牛的变化范围为0.68～1.00ng/ml。发情开始后192小时,正常母牛的血清孕酮水平已经达到2.47±2.02ng/ml,而屡配不孕母牛的血清孕酮水平则为1.97±0.60ng/ml。实验结果指示:体内内分泌失调可能是导致母牛屡配不孕的重要原因之一。 相似文献
12.
A field study was designed to compare ovarian response and embryo yield in cows during early lactation when gonadotropin administration followed one of four treatments. In group 1A (n = 19) and 1B (n = 9), the estrouses were synchronized by two prostaglandin F2alpha (PG) injections given 11 days apart, and starting from day 9 of the synchronized cycle superovulation was conducted with eight decreasing dose of FSH. In group 1B, ablation of all follicles >3 mm was carried out on day 8. In group 2A and 2B (each n = 9), a progesterone plus oestradiol intravaginal device (PRID) was inserted for 11 days and gonadotropin administration started on day 9, while cows from group 2B had a follicle ablation on day 8. In all groups, two PG injections were given along with the sixth and the seventh dose of FSH, and the cows were twice inseminated 12 and 24 h after estrus detection. Embryos were collected on day 7. In cumulative results from aspirated and non-aspirated cows, follicular ablation significantly improved: the ovarian response (10 +/- 1.23 vs 6.69 +/- 0.60 corpora lutea per donor), the mean collected embryos (6.57 +/- 0.94 vs 2.46 +/- 0.53) and the mean transferable embryos (4.43 +/- 0.89 vs 2.18 +/- 0.47). Group 1B and 2B cows had better ovarian response than 1A (6.44 +/- 0.81, 12.25 +/- 4.11 and 9.44 +/- 0.93, for groups 1A, 1B and 2B, respectively, p < 0.05). Similarly, from groups 1B and 2B more (p < 0.05) embryos were collected in comparison with their respective group, while the mean transferable embryos from group 2B (5.22 +/- 1.13) was greater (p < 0.05) than that of group 1A (1.67 +/- 0.35), and tented to be greater than those of groups 2A (3.44 +/- 1.19, p = 0.062) and 1B (3.00 +/- 1.78, p = 0.066). The highest (p < 0.05) transferable embryo collection rate was recorded in group 2B (55.29%), followed by that of group 1B (41.33%). In summary, early in lactation, an acceptable number of transferable embryos can be collected from high producing dairy cows, when follicle ablation prior to superovulation is combined with progesterone and oestradiol administration. 相似文献
13.
Hasan ALKAN Fatma SATILMIS Tahir KARASAHIN Sukru DURSUN Huseyin ERDEM 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):535
In this study, the effect of serum paraoxonase-1 (PON-1) activity on superovulation response and embryo yield was evaluated. The study material comprised 50 Holstein cows aged 3–4 years on postpartum day 90–120 with a body condition score of 3–3.25. A progesterone-based estrus synchronization protocol was initially administered to the selected donors. For this purpose, progesterone source was inserted intravaginally (day 0) and gonadotropin-releasing hormone injection was performed (day 6). Seven days after the insertion of progesterone device, follicle-stimulating hormone injections (total dose of 500 µg in decreasing doses for 4 days) were administered for superovulation. On the morning of the ninth day, prostaglandin (PG) F2α was administered, and the progesterone device was removed from the vagina in the evening on the same day. Two days after PGF2α administration, fixed-time artificial insemination was performed in the morning and in the evening. On the day of artificial insemination, blood samples were taken from the donors to determine the serum PON-1 activity. Uterine flushing was performed seven days after insemination. The results revealed that the serum PON-1 activity (mean ± SD, 562.71 ± 140.23 U/l) of the cows that responded to superovulation (donors with total corpus luteum count of ≥3 in both ovaries) was higher than those (389.91 ± 80.51 U/l) that did not (P<0.05). On the day of insemination, a positive correlation was determined between serum PON-1 activity and the counts of total corpus luteum (r=0.398), total oocyte/embryo (r=0.468), transferable embryo (r=0.453), and Code I embryos (r=0.315, P<0.05). Unlike the Code I embryos, there was no significant correlation between serum PON-1 activity and the number of Code III embryos. Moreover, no significant difference in the number of Code III embryos between the two PON-1 groups was observed. However, embryo yield and quality were found to have increased with increased PON-1 activity. Therefore, it was concluded that serum PON-1 activity may be associated with superovulation response, embryo yield and quality in donor cows. 相似文献
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微量滴定板酶免疫法和固相酶染色法分别检测试验组不孕母牛(n=119)和对照组母牛(n=26)包括处女牛、正常空怀母牛和妊娠母牛)的血清孕酮水平和抗精子抗体反应,以研究不孕母牛血清抗精子抗体反应与卵巢功能之间的关系。结果表明:各种原因不孕母牛的血清孕酮含量显著高于处女牛(P>0.05),但与正常空怀母牛和妊娠母牛比较无显著差异(P>0.05)。不孕母牛血清抗精子抗体阳性检出率显著高于对照组(P<0.01),但其抗精子抗体阳性反应血清(n=43)孕酮含量显著低于抗精子抗体阴性反应血清(n=76,P<0.05),而且孕酮含量与抗精子抗体阳性率呈负相关趋势。 相似文献
16.
Effects of Gn-RH on blood serum hormone concentrations, ovulation rates and embryo production in lactating cows treated with PMSG. 
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下载免费PDF全文 The effects of gonadotrophin releasing hormone injection into pregnant mare serum gonadotrophin treated cows on serum levels of luteinizing hormone, estradiol-17 beta and progesterone and ovulation rates was evaluated in 20 cows. Pregnant mare serum gonadotrophin treatment does not always induce superovulation as was shown in this study. Luteinizing hormone levels reached peak concentrations at 1.0 h and remained significantly (P less than or equal to 0.05) elevated at 2.0 h, 3.0 h, 4.0 h, and 7.0 h after the first gonadotrophin releasing hormone injection. Progesterone levels in the gonadotrophin releasing hormone treated group were significantly (P less than or equal to 0.05) higher than in the controls on the third and fourth days following injection. A significant correlation (r = 0.58-0.72) was noted between the number of corpus luteum and progesterone levels on each day between day 2 and day 7. More embryos were recovered (46 vs 26) from the ten treated than from the ten control cows. 相似文献
17.
Schernthaner W Wenigerkind H Stojkovic M Palma GA Mödl J Wolf E Brem G 《Zentralblatt für Veterin?rmedizin. Reihe A》1999,46(1):33-37
Thirty-six cows (21 Simmental, 5 Holstein-Frisian, 5 Brown Swiss and 5 Charolais) with high genetic superiority were punctured by ultrasound-guided follicle aspiration within the last 18 months for 503 times under equal conditions. Follicle aspiration was done twice per week. Most of the donor cows suffered from several disturbances of fertility. On average, 5 oocytes per session were collected. After in vitro maturation (IVM), fertilisation (IVF) and culture (IVC), 0.8 embryos per puncture session were transferred. After evaluation of the embryos by morphological criteria, these embryos were transferred to heifers that were oestrus synchronised (2.0 ml Estrumate i.m.) seven days after onset of oestrus. On day 21 after onset of oestrus the progesterone level in plasma was determined by radioimmunoassay. The pregnancy control was performed by ultrasound on day 35. After transfer of 397 embryos (to synchronised heifers), 125 pregnancies were established. Comparison of the different breeds and donor cows revealed significant differences in the number of oocytes recovered, embryos produced and pregnancies established. Transfer of embryos with insufficient morphological quality resulted in increased rates of embryonic loss between day 21 and day 35. 相似文献
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供体牛外周血AMH浓度对荷斯坦青年奶牛体内胚胎生产效率的影响 总被引：1，自引：0，他引：1
旨在研究供体牛超数排卵（简称超排）过程中不同时间外周血AMH浓度与荷斯坦青年奶牛体内胚胎生产效率的相关性,优化供体牛筛选标准,提高荷斯坦青年奶牛体内胚胎生产效率。本试验共选用96头荷斯坦青年奶牛进行超排处理,分别在孕酮阴道栓（CIDR）埋置、人工授精和胚胎回收当天通过尾根采集外周血检测抗缪勒管激素（anti-Müllerian hormone,AMH）浓度,将不同时期的外周血AMH浓度按照高（浓度分布最高的25%）、中等（浓度分布中间的25%~75%）、低（浓度分布最低的25%）分为3组,并检验了这3组之间头均回收胚胎数、头均可用胚胎数、头均退化胚胎数和头均未受精数等指标间的差异。结果表明：1）外周血AMH浓度检测结果显示,从CIDR埋置至胚胎回收的全过程AMH浓度整体呈下降趋势;2）CIDR埋置当天AMH高浓度组头均回收胚胎数（9.38±1.24）显著高于中等浓度组（6.47±0.56）（P<0.05）,说明CIDR埋置当天AMH浓度高的供体牛超排效果更好,回收胚胎数更多;但是,结果也表明,高浓度组头均未受精卵母细胞数显著高于其他两组（P<0.05）,说明部分卵母细胞未能成功受精,这可能是由于高浓度组牛只更多的卵泡优势化,排卵周期延长导致部分卵母细胞未能完成正常受精;3）人工授精和胚胎回收当天的AMH数据分析结果均表明,3组间各项胚胎生产指标均无显著性差异（P>0.05）;4）通过对供体牛体重记录数据分析发现,不同体重牛只间的体内胚胎生产效率差异也不显著（P>0.05）,但是体况适中组牛只的可用胚胎数、回收胚胎数优于其他两组,尤其是优于体重偏胖组。因此,CIDR埋置时,外周血AMH浓度可以作为青年荷斯坦供体牛筛选标准之一,且供体牛选择时宜选用体况适中的牛只。 相似文献
19.
在母牛产后第1次发情后第10d，给5头发情周期正常的母牛安装颈静脉血管导管；在母牛产后第2个发情周期进行挛生处理，即在产后第2次发情后第10d注射PMSG2000IU，2d后注射氯前列烯醇0．4mg第3次发情后注射抗PMSG抗体2000IU。自第1个发情周期第10d开始，每天间隔10min连续采血2h。发情当天间隔30min采血一次，直到第3次发情结束为止。挛生处理后采血间隔时间和方法与处理前不同。应用酶免疫测定方法测定血清中促性腺激素释放激素水平。结果表明：（1）牛外周血中GnRH释放具有脉冲性；（2)PMSG处理后外周血中GnRH水平升高，表现为脉冲频率增加，脉冲峰值升高。 相似文献
20.
The skim milk progesterone profile was assessed by radioimmunoassay, without extraction, from the day of insemination (day 0) until the cows were dried off on day 225 of gestation. A total of 418 samples were collected from 154 pregnant Holstein cows. The daily variation in skim milk progesterone was recorded from day 1 until day 45 of pregnancy to detect the commencement of progesterone secretion from the corpus luteum after insemination. Subsequent determinations were made every 2 weeks from day 46 until lactation ceased. On the day of artificial insemination and for the first 2 days after insemination, all the cows had a basal progesterone concentration <0.1 ng/ml. A rise in progesterone (0.2±0.1 ng/ml) was first detected on the third day after insemination. The progesterone values then increased significantly (p<0.001) until day 15.The values then remained nearly constant (2.5–3.5 ng/ml) until day 106 of pregnancy, when they began to decline. Between days 120 and 180 of gestation, progesterone was significantly decreased (2.2–2.9 ng/ml) before it rose again to the previous plateau (3.5–3.9 ng/ml) around day 180. The progesterone concentration then remained at the higher level until the animals were dried off.Abbreviations AI
artificial insemination
- RIA
radioimmunoassay 相似文献