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1.
《林业科学》2016,(5)
【目的】氮素是影响杨树人工林生产力的最重要元素,研究杨树人工林连作和轮作氮素循环细菌类群演变动态及氮素代谢结构特征,有助于从养分循环角度揭示杨树人工林连作障碍机制。【方法】采用宏基因组测序技术,研究杨树人工林Ⅰ代林地、连作Ⅱ代林地、Ⅱ代林地主伐后轮作花生地和轮荒地土壤中氮素循环细菌类群及氮素代谢随不同连作代数及不同轮作模式的演变规律。【结果】发现参与氮素循环细菌4类11属,其中固氮细菌有拜叶林克氏菌属、慢生根瘤菌、根瘤菌属和弗兰克氏菌属,硝化细菌有硝化杆菌属和亚硝化螺菌属,反硝化细菌有假单胞菌属、罗尔斯通菌属、伯克氏菌属、芽孢杆菌属和链霉菌属,氨化细菌有芽孢杆菌属和假单胞菌属;杨树人工林连作1代后,土壤中参与氮素循环细菌总数增加4.73%,土壤中氮素细菌的种类没有增减,固氮细菌的相对丰度增加53.44%,硝化细菌的相对丰度没有变化,反硝化细菌的相对丰度增加0.14%,氨化细菌的相对丰度增加1.33%;与Ⅱ代林相比,花生地土壤中的氮素细菌的种类没有增减,固氮细菌的相对丰度减少71.14%,硝化细菌、反硝化细菌和氨化细菌的相对丰度分别增加120%,15.63%和20.76%;轮荒地中的土壤氮素循环细菌缺少了硝化细菌,固氮细菌的相对丰度减少79.10%,反硝化细菌和氨化细菌的相对丰度分别增大17.39%和24.56%;杨树人工林连作1代后,土壤中的固氮细菌代谢活性增强,硝化细菌中的硝化杆菌属的代谢活性减弱,亚硝化螺菌属的代谢活性增强,氨化细菌代谢活性减弱;与Ⅱ代林相比,轮作花生地中仅有硝化细菌的代谢活性增加,其他3种氮素代谢功能菌的活性都降低;轮荒地中,所有的氮素循环细菌的代谢活性均比杨树Ⅱ代林地低。【结论】杨树人工林连作1代后,土壤中参与氮素循环的细菌总数增加,但代谢活性降低;轮作花生后,大多数氮素代谢细菌的数量增加,但仅有硝化细菌的代谢活性明显增强;轮作可以改善连作对杨树人工林地土壤硝化细菌生长繁殖和代谢活动的影响。 相似文献
2.
《山东林业科技》2015,(4)
对农林复合中杨树+留兰香、杨树+小麦、杨树+红薯和杨树纯林4种林地土壤微生物、杨树和间作物根际土壤微生物及根际效应进行了测定和分析。结果发现:与对照相比,间作显著增加了林地非根际和根际土壤微生物数量,尤其在杨树根际土壤中变化较大且无规律性,杨树+留兰香林地杨树根际土壤放线菌、氨化细菌数量最大,杨树+小麦林地杨树根际土壤好气性自生固氮菌、亚硝酸细菌数量最大,杨树+红薯林地杨树根际土壤真菌、纤维素分解菌数量最大;无论在间作林地还是杨树纯林,杨树具有较强的土壤微生物根际效应,其根际效应R/S值均大于1;但间作对其影响较大,土壤放线菌和亚硝酸细菌根际效应R/S值增大,土壤真菌、纤维素分解菌根际效应R/S值减小;土壤好气性固氮菌和氨化细菌因间作物不同而异。对于间作物来言,留兰香根际具有较强的吸引土壤微生物的能力和根际效应,留兰香根际土壤真菌、纤维素分解菌、氨化细菌、亚硝酸细菌数量和际效应R/S值均大于小麦和红薯,且留兰香的真菌、纤维素分解菌和亚硝酸细菌的根际效应R/S值均大于杨树。 相似文献
3.
《山东林业科技》2014,(6)
纤维素降解细菌(Cellulose-decomposing bacteria,CDB)能够利用难以分解的纤维素,对生物地球化学循环的推动具有重要意义。为探讨不同海拔高度、不同植被类型对CDB的分布特征及产酶能力的影响,选取云冷杉混交林、藓类冷杉林、蔷薇冷杉林、杜鹃冷杉林为研究对象,采用牛肉蛋白胨(NA)平板分离法、羧甲基纤维素(CMC)刚果红平板鉴别法,从4类冷杉林土样中分离CDB,并对其进行液体发酵,采用DNS法定量测定发酵上清液中的纤维素酶活。结果表明:(1)可培养的好氧细菌总数、CDB数随海拔升高而降低,与林下植物有极显著相关性(P0.01)。(2)CDB%(CDB数量占细菌总数的百分比)随海拔升高而升高,与林下植物有极显著正相关性(P0.01)。(3)H%(CDB菌株数占可培养细菌菌株数的百分比)与海拔、林下植物无明显相关关系,与凋落物厚度有一定负相关关系。(4)滤纸纤维素酶活(FPase)、羧甲基纤维素酶活(CMCase)均随海拔升高而升高。(5)4类冷杉林下产CMCase能力差异随海拔升高而显著。 相似文献
4.
[目的]研究间伐对杉木人工林土壤微生物数量、酶活性及关系的影响,试图了解不同间伐强度作用下土壤恢复的过程和机制,为人工林经营提供理论依据。[方法]以18年生杉木人工林为研究对象,采用随机区组试验设计,分析4种间伐强度TS0(未间伐(0.0%),1 800株·hm-2)、TS1(轻度(16.7%),1 500株·hm-2)、TS2(中度(33.3%),1 200株·hm-2)和TS3(重度(50.0%),900株·hm-2)下杉木人工林土壤微生物数量及土壤酶活性特点,探讨土壤微生物数量与酶活性的相关性。[结果]表明:间伐3年后,林下土壤层酶活性和微生物数量显著提高,不同土层间土壤微生物数量和酶活性均差异显著;间伐显著提高了土壤过氧化氢酶、碱性磷酸酶、脲酶和蔗糖酶的活性,除过氧化氢酶(15 30、30 45 cm)、碱性磷酸酶(0 15、30 45 cm)以及脲酶(30 45 cm)以TS3处理的酶活性最高外,其他酶活性在各土层和不同间伐强度下均以TS2处理的酶活性最高;土壤各层微生物以细菌数量最多,其次是放线菌,硝化细菌最少,且TS2处理的微生物数量最多。土壤过氧化氢酶和脲酶活性均与细菌、真菌和硝化细菌数量呈极显著正相关,与放线菌数量呈极显著负相关,氨化细菌数量与过氧化氢酶活性呈负相关,而与脲酶活性呈正相关;碱性磷酸酶活性与细菌、真菌和硝化细菌数量呈正相关,与氨化细菌数量呈极显著负相关,与放线菌数量呈负相关;蔗糖酶活性与细菌、真菌和氨化细菌数量呈极显著正相关,与硝化细菌数量呈正相关,与放线菌数量呈负相关。[结论]间伐改善了林分环境、光照、温度以及林下植被的发育,提高了林下土壤酶活性并增加了微生物数量。间伐3年后的综合表现表明,中度间伐最利于杉木人工中、近熟林阶段的经营,对于改善土壤性质较好。 相似文献
5.
杉木连栽林地土壤微生物区系及其生化特性和理化性质的研究 总被引：24，自引：0，他引：24
选择立地坏境条件相同的杉木连栽与头栽林地，进行土壤微生物，酶活性及理化性质的分析。研究表明，杉木连栽林地土壤微生物总数下降２３．３５％，其中细菌、放线菌分别下降２４．７９％和３４．０４％，真菌增加１０．３９％微生物主要生理类群氨化细菌、纤维素分解菌、好气固氮菌、微嗜氮菌分别下降３６．６０％、１４．２４％、５６．５６％和２５．８１％，厌气固氮菌增加４６．１５％．连栽林地土壤的脲酶、转化酶、过氧化氢酶的活性明显低于头栽土壤、多酚氧化酶则相反。在０－４０ｃｍ土层中．同于连栽引起土壤容重增加２．５９％、毛管孔隙、毛管含水量、田间持水量和自然含水量分别下降８．４６％、１１．０４％、１１．７８％和７．０８％，土壤有机质、Ｎ、Ｐ全量分别下降１６．６１％、１２．７５％和２１．４９％．Ｎ、Ｐ、Ｋ速效养分含量分别下降２０．０８％、８．７８％和２４．８５％．因而提出营造混交林，发展林下植被，停止或减少炼山和全垦以及增加肥料投入等技术对策。以防止连栽引起的地力衰退。 相似文献
6.
冀北山区不同植被恢复类型根际土壤细菌群落结构及多样性 总被引：1，自引：0，他引：1
【目的】研究冀北山区不同植被恢复类型对根际土壤细菌群落结构特征和多样性影响,探索自然和人工恢复对土壤微生物的作用机制,为该区域植被恢复与管理提供理论依据。【方法】以自然恢复的灌草丛、灌木林、次生林以及人工恢复的人工林根面、根际和非根际土为研究对象,通过Illumina Miseq平台对细菌16S rRNA的V3—V4片段进行高通量测序,分析土壤细菌α、β和功能多样性、群落结构组成以及与环境因子的关系。【结果】1)各恢复类型细菌α多样性差异显著(P0.05),次生林土壤细菌丰度和多样性均高于人工林,灌草丛多样性高,丰度最低;灌木林丰度高,多样性最低。不同根际范围土壤细菌α多样性差异不显著(P0.05),整体表现为根面和根际土高于非根际土。2)次生林和人工林土壤细菌群落结构有一定相似性;次生林与灌草丛、灌木林土壤细菌群落结构差异较大。根际土与非根际土细菌群落结构有一定相似性,根面土与根际、非根际土细菌结构差异较大。3)变形菌门、放线菌门、拟杆菌门、酸杆菌门、芽单胞菌门和厚壁菌门为优势类群。各样地有机质分解菌群(变形菌门、放线菌门和拟杆菌门)丰度差异显著,灌草丛和次生林显著高于其他样地,根面和根际土显著高于非根际土。固碳和固氮菌群(芽单胞菌门和厚壁菌门)分布较均匀。4)土壤田间持水量、有机质和全磷、植被分布均匀度、Simpson多样性指数和丰富度指数是影响土壤细菌群落的主要因素。土壤因子(田间持水量、有机质和全磷)对灌木林和灌草丛非根际土细菌影响极显著,对根面和根际土细菌作用较小;植被因子(植被分布均匀度、Simpson多样性指数和丰富度指数)对乔木林(次生林和人工林)不同根际范围土壤细菌群落影响均达到极显著水平。5)新陈代谢功能在KEGG上的编码基因数量最多,是土壤细菌的优势功能。各土样细菌群落KEGG功能基因序列数量和多样性差异显著,说明各植被恢复类型根际土壤中有许多具有各自独特功能的菌种,但土壤和植被不能决定土壤细菌功能多样性。【结论】恢复方式、植被类型和根系对冀北山区土壤细菌群落结构和多样性均有影响,其中恢复方式对土壤细菌多样性作用更明显,植被类型对土壤细菌群落结构影响更显著。距离根系越近,土壤细菌多样性越高,土壤细菌群落结构与非根际土差异越大。此外,环境因子如土壤养分、水分含量及植被分布状况等均与土壤细菌群落结构及多样性有关。 相似文献
7.
本研究就三种不同处理林分土壤微生物生理类群的初步分析,表明杉木马尾松混交后有利于固氮微生物的生长,固氮微生物数量的增加,促进了混交林分生长;有利于氨化细菌的生长,氨化细菌数量的增加,又利于提高含氮有机物的分解和转化速率,从而提高混交林地土壤肥力;同时促进了纤维素分解菌的生长,利于有机物的转化和加快碳素循环速率,从而为植物生长提供更多的碳素和能源。因此,在混交林中杉木的平均胸径比对照提高24%,平均树高比对照提高21%;马尾松平均胸径比对照提高20%,平均树高比对照提高19%,可见营造杉、马混交林比纯林更具发展潜力,可实现树高、胸径积累的双赢。 相似文献
8.
对地处澜沧江上游滇西北山区大理洱源炼铁乡的云南松林,旱冬瓜林,梨树果园,桉树林,桤木 圣诞树混交林5种主要林分类型林地土壤的氮含量与微生物学性质进行了研究.结果表明:不同林分类型林地土壤的含氮量与微生物学性质存在明显差异;林地土壤中的硝化细菌数量对其土壤氮含量的贡献大于氨化细菌的数量,但氨化作用对其土壤氮含量的贡献大于硝化作用的强弱;林地土壤氮含量的高低与其土壤的微生物学性质强弱一致,而受不同林分类型影响,两者间的关系是显著或极显著的,且相互影响相互联系.随林地土层的加深,其土壤微生物学性质、土壤氮含量均逐渐减少.5种林分类型林地土壤的微生物活性与土壤氮含量的排序为:旱冬瓜林＞桤木 圣诞树混交林＞梨树果园＞桉树林＞云南松林. 相似文献
9.
川西高山树线群落交错带地被物及土壤的水文效应 总被引：2，自引：0，他引：2
采用样带法,以川西米亚罗林区鹧鸪山高山树线群落交错带为研究对象,分析交错带地被物和土壤的持水特性及其变化规律。结果表明:地被物最大持水量为33.55～140.03t·hm-2,暗针叶林>林线>树线>密灌丛>草甸>疏灌丛,暗针叶林枯落物最大持水量显著高于疏密灌丛和草甸;林线地被物拦蓄降水的作用最明显;暗针叶林以苔藓层和枯落物层具有最大有效拦蓄效果,林线和树线以苔藓层具有最大有效拦蓄效果,疏密灌丛以枯落物层具有最大有效拦蓄效果,高山草甸草本层、苔藓层、枯落物层有效拦蓄效果相当;0～30cm土壤饱和蓄水量都在2000t·hm-2以上,暗针叶林>林线>疏灌丛>密灌丛≈草甸>树线;各群落0～30cm土层非毛管持水量为575.89～801.46t·hm-2,表现为暗针叶林>疏灌丛>树线>密灌丛>林线>草甸。 相似文献
10.
本文主要对柏木纯林、桤柏混交林凋落物分解袋地表土土壤微生物年动态变化进行分析。分析结果表明，在桤柏混交林内，细菌数量最多，放线菌次之，真菌最少；在柏木纯林中，细菌和放线菌数量基本相同，真菌较少。在两种林型中，三大微生物种群数量在３月最少，６～９月最多。微生物生理类群数量分析结果为桤柏混交林中的固氮菌、硝化菌、氨化细菌均大于柏木纯林。纤维分解菌类两者基本相同。柏木纯林反硝化菌类多于桤柏混交林。 相似文献
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【目的】采集杉木人工林土壤,通过室内土壤培养实验,研究添加不同制备原料和制备温度的生物炭对土壤细菌群落结构及多样性的影响,为改善南方酸性红壤及合理应用生物炭提供科学依据。【方法】原土添加3%的300℃杉叶炭(BL300)、600℃杉叶炭(BL600)、300℃木屑炭(BW300)及600℃木屑炭(BW600),与对照土壤进行对比,进行培养实验80天,运用高通量测序技术对PCR所扩增16SrDNA序列的V3+V4区域进行测定。【结果】OTU韦恩图分析表明,添加BL300的土壤细菌较对照丰度提高,其他生物炭处理丰度减小;通过PCoA分析和Beta多样性分析及UPGMA聚类分析得出,添加杉叶炭后土壤细菌群落结构及多样性与对照土壤的差异显著,其中BL600与对照差异最大,添加木屑炭结果与对照较相似;添加生物炭对不同物种水平上的土壤细菌结构和功能产生一定影响,其中杉叶炭处理影响十分显著,使土壤优势菌丰度变化较大,木屑炭处理对优势菌的影响相对较小。【结论】添加BL300生物炭提高了土壤细菌的丰度,而添加其他生物炭降低了细菌丰度;不同制备原料和温度对生物炭存在影响,由于木屑炭可利用氮素不足,杉叶生物炭对土壤细菌结构和多样性的影响比木屑生物炭更显著,高温炭灰分含量较多,因此对细菌多样性的影响大于低温炭;不同的土壤细菌种群生活习性与功能不同,对生物炭组分利用程度也不同,添加生物炭能够改变土壤中优势种群的相对丰度和土壤细菌群落的整体功能。 相似文献
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《林业研究》2020,31(5)
Castanopsis fargesii is a good afforestation plants and various microorganisms play important roles in mediating the growth and ecological functions of this species. In this study, we evaluated changes in microbial communities in soil samples from C. fargesii forests. The phospholipid fatty acid(PLFA) biomarker method was used to obtain bacteria, fungi, actinomycetes, gram-positive bacteria(G+), gram-negative bacteria(G-), aerobic bacteria, and anaerobic bacteria to investigate spatiotemporal changes in microbial communities during the growing season. The results show that soil microorganisms were mainly concentrated in the upper 20-cm layer, demonstrating an obvious surface aggregation(P 0.05). Large amounts of litter and heavy rainfall during the early growing season resulted in the highest PLFA contents for various microorganisms, whereas relatively low and stable levels were observed during other times. The dominant species during each period were bacteria. G+ or aerobic bacteria were the main bacterial populations,providing insights into the overall trends of soil bacterial PLFA contents. Due to the relative accumulation of refractory substances during the later stages of litter decomposition, the effects of fungi increased significantly.Overall, our findings demonstrate that the main factors influencing microbial communities were litter, rainfall, and soil field capacity. 相似文献
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攀枝花干热河谷区麻疯树土壤微生物种群数量分析 总被引：2，自引：0，他引：2
本文利用土壤微生物传统数量测定法,分析了攀枝花9个麻疯树主要分布区土壤微生物数量分布情况。分析结果表明,在三大微生物种群中,细菌占93.9%,丝状真菌和放线菌分别占3.1%和3.0%;不同林分年龄土壤微生物总数差异显著,成熟林分(1 237.6×104)>人工幼林(955.4×104);土壤类型不同,微生物种群数量差异明显,红壤(1 349.2×104)>黄沙壤(1 306.8×104)>山地黄壤(580.0×104)。通过土壤主要微生物生理类群分析结果,攀枝花麻疯树主要分布区土壤微生物生理类群数量比较贫乏,在氨化细菌数量中,红壤(37.0×103)>黄沙壤(0.8×103)>山地黄壤(0.3×103);在好气性纤维菌中,红壤(0.8×103)>黄沙壤(0.7×103)>山地黄壤(0.3×103);消化细菌,反消化细菌在山地黄壤和黄沙壤有少量分布;在3种土壤中均未分离到好气性固氮菌,说明攀枝花麻疯树主要分布区土壤不适宜好气性固氮菌生长繁殖。 相似文献
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Y.F. Cai P. Barber B. Dell P. O’Brien N. Williams B. Bowen G. Hardy 《Forest Ecology and Management》2010
This study investigates whether tree decline in Eucalyptus gomphocephala (tuart) is associated with the functional diversity of soil bacterial communities. We selected 12 sites with different stages of decline and assessed crown health [Crown density (CD), Foliage transparency (FT), Uncompacted live crown ratio (ULCR), Crown dieback ratio (CDR) and Epicormic index (EI)] and soil bacterial functional diversity based on Biolog EcoPlates™ incubation [Average well colour development (AWCD), Shannon diversity (H′), richness (S) and Shannon evenness (E)]. Crown health indices differed between sites with EI being the most robust indicator of decline in crown health followed by CDR and CD (P < 0.05). Soil bacterial indices collected at 0–10 and 20–30 cm soil depth between December (summer, dry season) and May (autumn, start of wet season) differed between sites (P < 0.05), and significant relationships between crown health indices, except ULCR, and all soil bacterial indices were observed. Principle component analysis (PCA) showed that a decrease in the utilization of carbohydrates, carboxylic acids, amino acids and amines by the soil bacterial communities correlated to sites with poor crown health, indicating some changes in physiological responses of bacterial groups with declining tree health. Using stepwise regression analyses, in the 0–10 cm soil layer in December, itaconic acid had a 46% contribution to the EI. Carboxylic acids, including itaconic acid, have a strong ability to solubilize soil minerals in calcareous soil, and these possibly increased the availability of soil mineral nutrients in the healthier sites compared to the declining sites, particularly in the dry season. In addition, lack of soil water in the declining sites limited soil bacterial diversity and was positively correlated with EI in the 0–10 cm soil layer in December. In conclusion, soil bacterial functional diversity has a strong relationship with tuart decline and the importance of soil microbes in tuart ecosystem health must be considered in the future. 相似文献
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以长江中游芦苇滩地为研究对象,利用微生物分离培养计数方法和PCR-DGGE技术重点研究了人工杨树林对芦苇滩地土壤细菌种群的影响。结果表明：与芦苇地相比,人工杨树林根际土、根表土和0-10cm土层中的氮、速效磷和总有机碳的含量都明显降低;人工杨树林下根际土中的微生物数量都比芦苇地的低,而其根表土中则是细菌、真菌数量低于芦苇地,放线具比芦苇地高;就4种功能细菌来说,人工杨树林根际土的纤维素菌、反硝化菌、硝化细菌和氨氧化细菌的数量则比芦苇地低,而根表土中除了氨氧化细菌外,其他3种功能细菌含量又比芦苇地的高。4种功能细菌数量在不同的土壤剖面中的分布也各不相同：在0-10cm土层中,人工杨树林的纤维素菌和氨氧化细菌的数量高于芦苇地,而其余2种功能茵则低于芦苇地;在10-25cm土层中,人工杨树林的纤维素菌和硝化细菌含量低于芦苇地,反硝化菌的含量却高于芦苇地,而氨氧化菌含量相同;在25-60cm土层中,除了纤维素菌,其他3种功能细菌含量都是人工杨树林低于芦苇地。通过系统进化树分析发现,滩地中普遍存在的细菌类群为α变形菌纲、β变形茵纲和硝化螺旋菌纲,而人工杨树林和芦苇地的根表土、根际土和0-10cm土层均存在各自专有的细菌种群,表明人工杨树林对芦苇滩地土壤中细菌种群产生了一定的影响。 相似文献
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《林业研究》2021,32(5)
In addition to bacteria,the contribution of fungi to nitrous oxide(N_2 O) production has been recognized but the responses of these two broad and unrelated groups of microorganisms to global environmental changes,atmospheric nitrogen(N) deposition,and precipitation in terms of N_2 O production are unclear.We studied how these two microbial-mediated N_2 O production pathways responded to soil moisture conditions and to N addition in an N-limited temperate forest.Soils from a long-term N addition experiment in Changbai Mountain,northeastern China were incubated.Varied concentrations of cycloheximide and streptomycin,both inhibitors of fungal and bacterial activity,were used to determine the contributions of both to N~2 O production in 66%,98% and 130% water-filled pore spaces(WFPS).The results showed that N_2 O production decreased significantly with increasing cycloheximide concentration whereas streptomycin was only inhibiting N_2 O emissions at 98% and 130% WFPS.The bacterial pathway of N_2 O production in N-addition(N_(add)) soil was significantly more dominant than that in untreated(N_(amb)) soil.The difference in the fungal pathway of N_2 O production between the soil with nitrogen addition and the untreated soil was not significant.Net N_2 O emissions increased with increasing soil moisture,especially at 130% WFPS,a completely flooded condition.Bacteria dominated carbon dioxide(CO_2) and N_2 O emissions in Nadd soil and at 130% WFPS regardless of N status,while fungi dominated CO_2 and N_2 O emissions in soil without N addition at 66% and 98% WFPS.The results suggest that flooded soil is an important source of N_2 O emissions and that bacteria might be better adapted to compete in fertile soils under anoxic conditions. 相似文献
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The possible horizontal transfer of transgenes is of great concern when the transgenic plants are released into the field. To test the possible transfer of nptⅡ of transgenic trees into soil bacteria, we have used a stool DNA preparation kit to isolate the DNA from the soils in the rhizospheres of two non- and eight transgenic Eucalyptus camaldulensis trees. All the samples have provided the corresponding PCR products in the amplification with bacterial 16S RNA specific sequences, which indicates that the quality of the isolated DNA is adequate for amplification. The nptⅡ specific band has been amplified in three soil samples from the transgenic trees and even treated with filtration before the DNA isolation. This indicates that nptlI DNA exists in the soil, although it is still unclear whether the DNA was in the soil particles, in the soil bacteria or in the Agrobaeterium contamination which was used for the E.camaldulensis transformation. Two approaches on isolation of bacterial DNA have been suggested for testing the possibility of this event in the future. 相似文献
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Katsuaki ISHII 《中国林学(英文版)》2008,10(2):134-136
The possible horizomal transfer of transgenes is of great concern when the transgenic plants are released imo the field. To test the possible transfer of nptII of transgenic trees into soil bacteria, we have used a stool DNA preparation kit to isolate the DNA from the soils in the rhizospheres of two non- and eight transgenic Eucalyptus camaldulensis trees. All the samples have provided the corresponding PCR products in the amplification with bacterial 16S RNA specific sequences, which indicates that the quality of the isolated DNA is adequate for amplification. The nptⅡ specific band has been amplified in three soil samples from the transgenic trees and even treated with filtration before the DNA isolation. This indicates that nptII DNA exists in the soil, although it is still unclear whether the DNA was in the soil particles, in the soil bacteria or in the Agrobacterium comamination which was used for the E. camaldulensis transformation. Two approaches on isolation of bacterial DNA have been suggested for testing the possibility of this event in the future. 相似文献