共查询到20条相似文献，搜索用时 15 毫秒
1.
Human recombinant granulocyte-macrophage colony-stimulating factor: a multilineage hematopoietin 总被引：33，自引：0，他引：33
C A Sieff S G Emerson R E Donahue D G Nathan E A Wang G G Wong S C Clark 《Science (New York, N.Y.)》1985,230(4730):1171-1173
Human recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) was tested for its ability to induce colony formation in human bone marrow that had been enriched for progenitor cells. In addition to its expected granulocyte-monocyte colony-stimulating activity, the recombinant GM-CSF had burst-promoting activity for erythroid burst-forming units and also stimulated colonies derived from multipotent (mixed) progenitors. In contrast, recombinant erythroid-potentiating activity did not stimulate erythroid progenitors. The experiments prove that human GM-CSF has multilineage colony-stimulating activity. 相似文献
2.
Macrophages have been generated from bone marrow progenitor cells （BMPCs） with macrophage colony-stimulating factor （M-CSF）. Rapamycin （rapa） is an immunosuppressive drug, which could prevent renal graft rejection and inhibit tumor growth to yield antiproliferative activity in a variety of malignancies. However, the direct effect of rapa on the development of macrophages is unknown. In this study, we explored the direct effect of rapa on the differentiation and function of macrophages differentiated from mouse BMPCs in vitro in the presence of M-CSF. The experimental data showed that rapa prevented the differentiation of macrophagcs by down-regulating CD80 and CD86 expression but upregulating F4/80 expression, as well as by reducing the capacity of differentiated macrophages to stimulate lymphocyte proliferation in the allogeneic mixed lymphocyte reaction. Furthermore, the phagocytic capacity of differentiated macrophages was significantly reduced by rapa. Therefore, rapa may directly inhibit macrophage differentiation and function, which may have been one of the major targets for rapa to mediate its immunosuppressive properties. 相似文献
3.
A macrophage-derived factor required by plasmacytomas for survival and proliferation in vitro 总被引：40，自引：0，他引：40
Plasmacytoma (PCT) cell lines dependent for proliferation and survival on a factor elaborated by the murine macrophage cell line, P388D1, were established in vitro. Adherent peritoneal cells induced by pristane produced 50-fold greater amounts of this activity in vitro than did resident cells. The molecules responsible for plasmacytoma growth were distinct from a number of characterized factors including interleukin-1, -2, and -3, macrophage colony-stimulating factor, B-cell stimulatory factor-1, B-cell growth factor II, epidermal growth factor, transforming growth factor-beta, and gamma- and beta-interferon, none of which were able to support the growth of the factor-dependent PCT cell lines. These results suggest that PCT growth factor may be a novel factor that has not been previously characterized and, further, that its production is associated with the pristane-induced, chronic peritoneal inflammatory response that precedes plasmacytoma formation. 相似文献
4.
Endogenous regulation of macrophage proliferative expansion by colony-stimulating factor-induced interferon 总被引：19，自引：0，他引：19
Stimulation of cultures of murine bone-marrow cells with specific macrophage growth factor (colony-stimulating factor I) resulted in the production of type I interferon. Neutralization of this endogenous interferon by antiserum directed against interferons alpha and beta resulted in a significant enhancement of mononuclear phagocyte proliferation from committed marrow precursors. The effect of the antiserum was lost in cultures depleted of adherent cells, an indication that an adherent regulatory cell (or cells) in the marrow limits mononuclear phagocyte proliferation by producing antiproliferative interferon in response to high levels of specific growth factor. 相似文献
5.
Cytokines alter production of HIV-1 from primary mononuclear phagocytes 总被引：58，自引：0，他引：58
Y Koyanagi W A O'Brien J Q Zhao D W Golde J C Gasson I S Chen 《Science (New York, N.Y.)》1988,241(4873):1673-1675
Some strains of human immunodeficiency virus type 1 (HIV-1) can infect primary monocytes and monocyte-derived macrophages in vitro. In this report, the effect of cytokines on the production of one of these strains that shows a tropism for mononuclear phagocytes, designated HIV-1JR-FL, was studied. Primary peripheral blood mononuclear phagocytes infected with HIV-1JR-FL were treated with the hematopoietic factors: granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3), macrophage colony-stimulating factor (M-CSF), and gamma-interferon (gamma-IFN). The M-CSF, GM-CSF, IL-3, and gamma-IFN were able to alter HIV-1 production under different conditions. 相似文献
6.
Purified human granulocyte-macrophage colony-stimulating factor: direct action on neutrophils 总被引：57，自引：0，他引：57
J C Gasson R H Weisbart S E Kaufman S C Clark R M Hewick G G Wong D W Golde 《Science (New York, N.Y.)》1984,226(4680):1339-1342
Neutrophil migration inhibition factor from T lymphocytes (NIF-T) is a lymphokine that acts to localize granulocytes. Medium conditioned by the Mo human T-lymphoblast cell line was used to purify NIF-T, a glycoprotein with a molecular weight of 22,000. The NIF-T was found to potently stimulate the growth of granulocyte and macrophage colonies from human bone marrow and colony formation by the KG-1 myeloid leukemia cell line. Thus a human lymphokine (NIF-T) that modulates the activities of mature neutrophilic granulocytes is also a colony-stimulating factor acting on precursors to induce growth and differentiation of new effector cells. 相似文献
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8.
粒细胞-巨噬细胞集落刺激因子(GM-CSF)是机体免疫系统的重要细胞因子,具有生物佐剂作用,为研究GM-CSF的生物佐剂作用,本试验通过RT-PCR方法从小鼠脾脏细胞中扩增小鼠GM-CSF的cDNA,并将其插入到pcDNA3.1质粒中,构建成GM-CSF真核表达载体pcDNA3.1-GMCSF,并在真核细胞进行了瞬时表达。结果表明,本研究扩增的小鼠GM-CSF基因序列与GenBank序列完全一致,表达载体经脂质体介导转染HEK293T细胞,表达产物经western-blot检测,证明GM-CSF能够在HEK293T细胞中进行分泌表达。这为今后研究GM-CSF在动物疫苗,特别是DNA疫苗中的生物佐剂作用创造了必要的条件。 相似文献
9.
Molecular cloning of a complementary DNA encoding human macrophage-specific colony-stimulating factor (CSF-1) 总被引：65，自引：0，他引：65
E S Kawasaki M B Ladner A M Wang J Van Arsdell M K Warren M Y Coyne V L Schweickart M T Lee K J Wilson A Boosman 《Science (New York, N.Y.)》1985,230(4723):291-296
Complementary DNA (cDNA) clones encoding human macrophage-specific specific colony-stimulating factor (CSF-1) were isolated. One cDNA clone codes for a mature polypeptide of 224 amino acids and a putative leader of 32 amino acids. This cDNA, which was cloned in the Okayama-Berg expression vector, specifies the synthesis of biologically active CSF-1 in COS cells, as determined by a specific radioreceptor assay, macrophage bone marrow colony formation, and antibody neutralization. Most of the cDNA isolates contain part of an intron sequence that changes the reading frame, resulting in an abrupt termination of translation; these cDNA's were inactive in COS cells. The CSF-1 appears to be encoded by a single-copy gene, but its expression results in the synthesis of several messenger RNA species, ranging in size from about 1.5 to 4.5 kilobases. 相似文献
10.
Hsu CL Lin W Seshasayee D Chen YH Ding X Lin Z Suto E Huang Z Lee WP Park H Xu M Sun M Rangell L Lutman JL Ulufatu S Stefanich E Chalouni C Sagolla M Diehl L Fielder P Dean B Balazs M Martin F 《Science (New York, N.Y.)》2012,335(6064):89-92
Lysosomal storage diseases (LSDs) are a group of heterogeneous disorders caused by defects in lysosomal enzymes or transporters, resulting in accumulation of undegraded macromolecules or metabolites. Macrophage numbers are expanded in several LSDs, leading to histiocytosis of unknown pathophysiology. Here, we found that mice lacking the equilibrative nucleoside transporter 3 (ENT3) developed a spontaneous and progressive macrophage-dominated histiocytosis. In the absence of ENT3, defective apoptotic cell clearance led to lysosomal nucleoside buildup, elevated intralysosomal pH, and altered macrophage function. The macrophage accumulation was partly due to increased macrophage colony-stimulating factor and receptor expression and signaling secondary to the lysosomal defects. These studies suggest a cellular and molecular basis for the development of histiocytosis in several human syndromes associated with ENT3 mutations and potentially other LSDs. 相似文献
11.
The human gene encoding GM-CSF is at 5q21-q32, the chromosome region deleted in the 5q- anomaly 总被引：16，自引：0，他引：16
K Huebner M Isobe C M Croce D W Golde S E Kaufman J C Gasson 《Science (New York, N.Y.)》1985,230(4731):1282-1285
Human granulocyte-macrophage colony-stimulating factor (GM-CSF) is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. A full-length complementary DNA clone encoding human GM-CSF was used as a probe to screen a human genomic library and isolate the gene encoding human GM-CSF. The human GM-CSF gene is approximately 2.5 kilobase pairs in length with at least three intervening sequences. The GM-CSF gene was localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. An established, human promyelocytic leukemia cell line, HL60, contains a rearranged, partially deleted GM-CSF allele and a candidate 5q- marker chromosome, indicating that the truncated GM-CSF allele may reside at the rejoining point for the interstitial deletion on the HL60 marker chromosome. 相似文献
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13.
Bone resorption by osteoclasts 总被引：2，自引：0，他引：2
Teitelbaum SL 《Science (New York, N.Y.)》2000,289(5484):1504-1508
Osteoporosis, a disease endemic in Western society, typically reflects an imbalance in skeletal turnover so that bone resorption exceeds bone formation. Bone resorption is the unique function of the osteoclast, and anti-osteoporosis therapy to date has targeted this cell. The osteoclast is a specialized macrophage polykaryon whose differentiation is principally regulated by macrophage colony-stimulating factor, RANK ligand, and osteoprotegerin. Reflecting integrin-mediated signals, the osteoclast develops a specialized cytoskeleton that permits it to establish an isolated microenvironment between itself and bone, wherein matrix degradation occurs by a process involving proton transport. Osteopetrotic mutants have provided a wealth of information about the genes that regulate the differentiation of osteoclasts and their capacity to resorb bone. 相似文献
14.
Yarovinsky F Zhang D Andersen JF Bannenberg GL Serhan CN Hayden MS Hieny S Sutterwala FS Flavell RA Ghosh S Sher A 《Science (New York, N.Y.)》2005,308(5728):1626-1629
Mammalian Toll-like receptors (TLRs) play an important role in the innate recognition of pathogens by dendritic cells (DCs). Although TLRs are clearly involved in the detection of bacteria and viruses, relatively little is known about their function in the innate response to eukaryotic microorganisms. Here we identify a profilin-like molecule from the protozoan parasite Toxoplasma gondii that generates a potent interleukin-12 (IL-12) response in murine DCs that is dependent on myeloid differentiation factor 88. T. gondii profilin activates DCs through TLR11 and is the first chemically defined ligand for this TLR. Moreover, TLR11 is required in vivo for parasite-induced IL-12 production and optimal resistance to infection, thereby establishing a role for the receptor in host recognition of protozoan pathogens. 相似文献
15.
Parasympathetic innervation maintains epithelial progenitor cells during salivary organogenesis 总被引：1，自引：0，他引：1
Knox SM Lombaert IM Reed X Vitale-Cross L Gutkind JS Hoffman MP 《Science (New York, N.Y.)》2010,329(5999):1645-1647
The maintenance of a progenitor cell population as a reservoir of undifferentiated cells is required for organ development and regeneration. However, the mechanisms by which epithelial progenitor cells are maintained during organogenesis are poorly understood. We report that removal of the parasympathetic ganglion in mouse explant organ culture decreased the number and morphogenesis of keratin 5-positive epithelial progenitor cells. These effects were rescued with an acetylcholine analog. We demonstrate that acetylcholine signaling, via the muscarinic M1 receptor and epidermal growth factor receptor, increased epithelial morphogenesis and proliferation of the keratin 5-positive progenitor cells. Parasympathetic innervation maintained the epithelial progenitor cell population in an undifferentiated state, which was required for organogenesis. This mechanism for epithelial progenitor cell maintenance may be targeted for organ repair or regeneration. 相似文献
16.
旨在建立C57BL/6小鼠骨髓源CD103+树突状细胞(CD103+ dendritic cell,CD103+DC)分离培养方法,阐述LPS对其形态与功能特征的影响。在无菌条件下分离C57BL/6小鼠骨髓细胞,并用重组GM-CSF和FLT3L对其进行体外联合诱导培养;利用光镜、扫描电镜、荧光显微镜和流式细胞术,分别对LPS作用前后细胞形态、表型及功能进行了分析。结果表明,细胞培养至第3天有零星集落出现,第13天后集落开始分散,可见典型的树突状突起,第15天后可得到大量的CD103+DC,加LPS刺激培养24 h后细胞表面树突样结构更加明显;分离培养的骨髓细胞能够表达表面分子CD103,其表达率达90%以上。RPMI-1640组(LPS未刺激组)可吞噬VOA的CD103+DC比例为25.70%,能够表达MHC-Ⅱ和CD83阳性细胞分别为41.31%和13.79%;LPS刺激组可吞噬VOA的CD103+DC比例为10.33%,能够表达MHC-Ⅱ和CD83的阳性细胞分别为68.10%和24.71%。MTT法检测结果显示,经LPS处理的CD103+DC刺激T细胞增殖的能力明显增强。综上所述,分离于C57BL/6小鼠的骨髓细胞,可在体外经FLT3L和GM-CSF共同诱导培养出CD103+DC,LPS可促进CD103+DC的成熟。 相似文献
17.
对肉鸡骨髓红系和粒单系祖细胞集落形成单位（ＣＦＵ－Ｅ和ＣＦＵ—ＧＭ的培养条件和生长情况进行了探讨，并观察了不同日龄肉鸡骨髓造血祖细胞体外增殖能力。结果表明：（１）培养体系中加入灭活鸡血清，其集落产率较加小牛血清为高，其生长分裂也迅速，说明同源血清优于异源血清。（２）肉鸡骨髓ＣＦＵ—Ｅ培养体系最适ｐＨ值为７．８～８，０；ＣＦＵ—ＧＭ最适ｐＨ值为７．２～７．３。（３）试验使用鸡脾细胞条件培养液作为集落刺激因子，ＣＦＵ—ＧＭ生长良好。（４）７日龄肉鸡ＣＦＵ—Ｆ为１５３±８．１５，ＣＦＵ—ＧＭ为１６２±３．９４．４２日龄肉鸡ＣＦＵ—Ｅ为６２±２．１２，ＣＦＵ—ＧＭ为８３±３．２４。两种日龄红系、粒系集落产率均有明显差异（Ｐ＜０．０１），表明肉鸡骨髓造血祖细胞功能状态不同，呈现日龄差异。 相似文献
18.
Erythropoietin retards DNA breakdown and prevents programmed death in erythroid progenitor cells 总被引：62，自引：0，他引：62
The mechanism by which erythropoietin controls mammalian erythrocyte production is unknown. Labeling experiments in vitro with [3H]thymidine demonstrated DNA cleavage in erythroid progenitor cells that was accompanied by DNA repair and synthesis. Erythropoietin reduced DNA cleavage by a factor of 2.6. In the absence of erythropoietin, erythroid progenitor cells accumulated DNA cleavage fragments characteristic of those found in programmed cell death (apoptosis) by 2 to 4 hours and began dying by 16 hours. In the presence of erythropoietin, the progenitor cells survived and differentiated into reticulocytes. Thus, apoptosis is a major component of normal erythropoiesis, and erythropoietin controls erythrocyte production by retarding DNA breakdown and preventing apoptosis in erythroid progenitor cells. 相似文献
19.
Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells 总被引：77，自引：0，他引：77
L M Souza T C Boone J Gabrilove P H Lai K M Zsebo D C Murdock V R Chazin J Bruszewski H Lu K K Chen 《Science (New York, N.Y.)》1986,232(4746):61-65
Experiments were conducted to isolate and characterize the gene and gene product of a human hematopoietic colony-stimulating factor with pluripotent biological activities. This factor has the ability to induce differentiation of a murine myelomonocytic leukemia cell line WEHI-3B(D+) and cells from patients with newly diagnosed acute nonlymphocytic leukemia (ANLL). A complementary DNA copy of the gene encoding a pluripotent human granulocyte colony-stimulating factor (hG-CSF) was cloned and expressed in Escherichia coli. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay. In addition, recombinant hG-CSF can support early erythroid colonies and mixed colony formation. Competitive binding studies done with 125I-labeled hG-CSF and cell samples from two patients with newly diagnosed human leukemias as well as WEHI-3B(D+) cells showed that one of the human leukemias (ANLL, classified as M4) and the WEHI-3B(D+) cells have receptors for hG-CSF. Furthermore, the murine WEHI-3B(D+) cells and human leukemic cells classified as M2, M3, and M4 were induced by recombinant hG-CSF to undergo terminal differentiation to macrophages and granulocytes. The secreted form of the protein produced by the bladder carcinoma cell line 5637 was found to be O-glycosylated and to have a molecular weight of 19,600. 相似文献
20.
Trombetta ES Ebersold M Garrett W Pypaert M Mellman I 《Science (New York, N.Y.)》2003,299(5611):1400-1403
In response to a variety of stimuli, dendritic cells (DCs) transform from immature cells specialized for antigen capture into mature cells specialized for T cell stimulation. During maturation, the DCs acquire an enhanced capacity to form and accumulate peptide-MHC (major histocompatibility complex) class II complexes. Here we show that a key mechanism responsible for this alteration was the generalized activation of lysosomal function. In immature DCs, internalized antigens were slowly degraded and inefficiently used for peptide loading. Maturation induced activation of the vacuolar proton pump that enhanced lysosomal acidification and antigen proteolysis, facilitating efficient formation of peptide-MHC class II complexes. Lysosomal function in DCs thus appears to be specialized for the developmentally regulated processing of internalized antigens. 相似文献