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1.
An 8‐week feeding trial was conducted to evaluate the effects of dietary nucleotide (NT)‐rich yeast supplementation on growth, innate immunity and intestinal morphology in Pacific white shrimp (Litopenaeus vannamei). Four isonitrogenous and isolipidic practical diets were formulated to contain 0 (control), 10, 30 and 50 g/kg of NT‐rich yeast, respectively. A total of 480 shrimp with an average initial body weight of 1.86 ± 0.02 g were randomly allocated into four groups, with four replicates per group and 30 shrimp each replicate. The results indicated that shrimp fed the diet containing 50 g/kg NT‐rich yeast had significantly higher weight gain (WG), specific growth rate (SGR) and protein efficiency ratio (PER) than those fed the control diet, and the lowest feed conversion ratio (FCR) was observed in the shrimp fed the 50 g/kg NT‐rich yeast supplemental diet. However, there was no significant difference in survival among all treatments. The crude protein of whole shrimp in the 50 g/kg NT‐rich yeast group was higher than that in the control group. Total protein, triglyceride concentrations, the activities of aspartate aminotransferase and alanine aminotransferase in serum were significantly influenced by the dietary NT‐rich yeast supplementation. The activities of serum phenoloxidase (PO) and lysozyme (LZM) of shrimp fed the diet containing 50 g/kg NT‐rich yeast were higher than those in shrimp fed the other diets. Relative expressions of alp and lzm significantly upregulated in the 30 g/kg NT‐rich yeast group compared to the control group. The intestinal fold height and fold width in the 30 g/kg NT‐rich yeast group were significantly higher than those fed the control diet; and the highest microvillus height occurred in the shrimp fed the 50 g/kg NT‐rich yeast diet. In summary, dietary 30–50 g/kg NT‐rich yeast supplementation promotes growth performance, enhances innate immunity and improves intestinal morphology of Litopenaeus vannamei.  相似文献   

2.
The main objective of this study was to evaluate the effect of methionine supplementation when reducing fishmeal levels in diets for white shrimp (Litopenaeus vannamei). Tested diets consisted of a positive control with 260 g/kg fishmeal (D1), two negative controls with 100 g/kg fishmeal and no amino acid (AA) supplementation (D2) or supplemented with lysine but not methionine (D3), and four additional diets with 100g/kg fishmeal supplemented with increasing levels of DL‐Met (1.0, 2.0 or 3.0 g/kg) (D4, D5, D6) or Met‐Met (1.0 g/kg) (D7). Each diet was fed to four groups of 30 shrimp for 8 weeks at a daily rate of 70 g/kg body weight. Reduction in fishmeal from 260 g/kg down to 100 g/kg did not significantly affect survival rate, feed conversion ratio (FCR), protein efficiency ratio (PER) or protein retention efficiency (PR%) of white shrimp. However, growth performance (final body weight, FBW; weight gain, WG; specific growth rate, SGR) was reduced when dietary fishmeal level was reduced from 260 g/kg (D1) to 100 g/kg without methionine supplementation (D2). The growth performance (FBW, WG and SGR) of shrimp was significantly increased by supplementation of the 100 g/kg fishmeal diet with increasing levels of DL‐Met (p < .05). Same performance as positive control (D1) was achieved with diets containing 100 g/kg fishmeal and supplemented with 3.0 g/kg DL‐Met or 1.0 g/kg Met‐Met. The highest values of growth performance (FBW, WG and SGR) were found in shrimp fed D6 and D7 diets, which were significantly higher than those of shrimp fed D2 and D3 diets (p < .05) but without statistical differences with shrimp fed D1, D4 and D5 diets (p > .05). The highest values of whole‐body and muscle protein contents were found in shrimp fed D1 diet, which were significantly higher than those of shrimp fed all other diets (p < .05). The highest value of intestinal tract proteolytic enzyme activity was found in shrimp fed Met‐Met‐supplemented diet (D7) and followed by the positive control diet (D1) and 3 g/kg DL‐Met‐supplemented diet (D6) (p < .05). The highest values of apparent digestibility coefficients (ADCs) of dry matter and crude protein were found in Met‐Met‐supplemented diet (D7) and followed by the positive control diet (D1) (p < .05). Shrimp fed the D1 diet showed the highest value of total essential amino acid (EAA) and was significantly higher than shrimp fed D2–D3 (p < .05) but without significant difference with shrimp fed D4–D7 (p > .05). In conclusion, results showed that same performance can be achieved with diets containing 260 or 100 g/kg fishmeal supplemented with 3.0 g/kg DL‐Met or 1.0 g/kg Met‐Met. Moreover, supplementation of limiting methionine in low‐fishmeal diets seems to improve the digestive proteolytic activity, improving digestibility of dry matter and protein, and eventually to promote growth of juvenile white shrimp in fishmeal reduction diets.  相似文献   

3.
This study investigated the effects of coconut oil as a dietary supplement on the growth, lipid metabolism and related gene expressions of juvenile orange‐spotted grouper Epinephelus coioides. Coconut oil at concentrations of 0, 10, 30 and 50 g/kg was used to replace dietary lipids in a basal diet containing 150 g/kg lipids. The four experimental diets were, respectively, fed to triplicate groups of juvenile groupers (initial weight: 8.53 ± 0.13 g) in a recirculating system for 8 weeks. Fish fed the diet containing 50 g/kg coconut oil exhibited lower (p < .05) weight gain than did fish fed the diet containing 30 g/kg coconut oil; however, no significant differences in weight gain were observed between fish fed diets containing 0 and 10 g/kg coconut oil. Hepatic carnitine palmitoyltransferase‐1, fatty acid synthase, fatty acid elongase, fatty acid desaturase and peroxisome proliferator‐activated receptor gamma gene expressions were all the highest in fish fed the diet containing 10 g/kg coconut oil. Fish fed the coconut oil‐free basal diet demonstrated upregulated gene expression of neuropeptide Y. The results suggest that dietary supplementation with 10 g/kg coconut oil exerted beneficial effects on lipid metabolism by E. coioides.  相似文献   

4.
A 3‐hr experiment was conducted to investigate the effects of dietary myo‐inositol (MI) supplementation on survival, immune response and antioxidant abilities in Litopenaeus vannamei under acute hypoxia stress. Six practical diets were formulated with supplementation of graded levels (control group 0, 0.1, 0.2, 0.4, 0.8 and 1.6 g/kg dry diet) of MI and were randomly assigned to triplicate groups of L. vannamei (mean weight 0.40 ± 0.00 g) for 8 weeks. Ten healthy shrimp (final mean weight approximately 11–14 g) randomly selected from each tank were exposed to hypoxia stress after feeding trial. After 3‐hr acute hypoxia stress, survival of shrimp fed MI‐supplemented diets (except 0.1 and 0.4 g/kg diets) was significantly increased compared with the control group. Shrimp fed control diet had lower activities of alkaline phosphatase (AKP), acid phosphatase (ACP), total antioxidant capacity (T‐AOC) and glutathione peroxidase (GPX), and higher malondialdehyde (MDA) and protein carbonyl (PC) contents in hepatopancreas than those fed the MI‐supplemented diets. In addition, mRNA expression levels of heat shock protein 70 (Hsp70), catalase (CAT) and penaeidin were significantly differentially regulated in hepatopancreas. In summary, dietary MI supplementation may have a positive effect on improving resistance to acute hypoxia stress of L. vannamei.  相似文献   

5.
An 8‐week feeding trial was conducted with extruded practical diets containing 320 g/kg of crude protein from plant‐based sources to determine the effects of L‐lysine on growth performances, muscle‐growth‐related gene (myoD, myogenine and myostatin) expression and haemato‐biochemical responses in juvenile genetically improved farmed tilapia (GIFT). Five isonitrogenous and isoenergetic diets were formulated to contain graded levels of lysine (14.3, 16, 17.5, 19 and 20.5 g/kg of diet). Each diet was randomly assigned to triplicate groups of 30 juvenile fish (5.2 ± 0.1 g), which were fed thrice a day (9:00, 13:00 and 17:00 hr). Maximum growth performances were observed in fish fed with lysine at 19 g/kg of diet. There was no significant (p > .05) effect on whole‐body composition and amino acids profile by dietary lysine supplementation. Significant (p < .05) changes were observed in relative expression of muscle‐growth‐related genes namely myoD and myogenine and in plasma metabolites by dietary lysine supplementation. In contrast, the relative expression of myostatin was not affected by dietary lysine supplementation. Broken‐line regression analysis and second‐order polynomial regression analysis of weight gain and N gain against dietary lysine levels showed that the dietary lysine requirement for juvenile GIFT tilapia was 18–18.3 g/kg of diet and 19.3–19.5 g/kg of diet, respectively.  相似文献   

6.
The effects of dietary administration of inorganic zinc (zinc sulphate, ZnSO4) and nano zinc (zinc oxide nanoparticles, ZnO‐NP) were evaluated in rohu, Labeo rohita fingerlings. Fish were fed with a basal diet (Control) supplemented with ZnSO4 (T1, T2 and T3) and ZnO‐NP (T4, T5 and T6) at 10, 20 and 30 mg/kg, respectively, for a duration of 45 days. The results revealed that fish fed diet containing 20 mg ZnO‐NP per kg (T5) had the highest weight gain and specific growth rate (SGR, % per day), which was significantly different (p < .05) from the other experimental diets. Significantly (p < .05), higher activities of the digestive and metabolic enzymes were recorded in the fish fed ZnO‐NP containing diets as compared to the diets containing inorganic Zn or control diet. The maximum serum glucose and protein levels were noted in fish reared on diet T5. Both SGOT and SGPT activities were significantly increased in fish fed Zn‐supplemented diets (T1 to T6), as compared to the control group. Similarly, innate immune parameters were improved with feeding Zn incorporated diets. The highest phagocytic (40.74 ± 0.65%) and respiratory burst (0.33 ± 0.001, OD 630nm) activities were recorded in the fish fed diet containing ZnO‐NPs at 20 mg/kg (T5). The maximum superoxide production and serum peroxidase activity were detected in the fish fed T5 and T6 diets. Overall, results indicated that short‐duration feeding (≤45 days) of dietary ZnO‐NP (20 mg/kg) improved growth, enzyme activity, serum biochemical parameters and immune function in rohu fingerlings.  相似文献   

7.
A feeding trial was conducted to evaluate the effect of pawpaw–onion powder (POP) mixture on the growth, and haemato‐biochemical and antioxidant responses of Clarias gariepinus (4.02 ± 0.01g/fish) for 60 days. Five trial diets were formulated as control (without POP or antimicrobial growth promoter (AGP)), AGP (basal diet + 10ml AGP/kg diet), POP 2.5 (basal diet + 2.5g POP/kg diet), POP 5.0 (basal diet + 5.0g POP/kg diet) and POP 10 (basal diet + 10g POP/kg diet). Two hundred and twenty‐five fish were equally distributed into five groups in triplicate and fed twice daily. The results indicate that AGP or POP supplementation exerted no effects on the growth and blood profile among the various groups, but a significantly higher lymphocyte count was observed in POP 10g/kg. The highest whole‐body protein and lipid contents were noticed in fish fed the control diet (p < .05), whereas POP 5.0g/kg group recorded the highest hepatosomatic value. Furthermore, the cholesterol level was found to be lower in the AGP‐ and POP‐fed fish compared with the higher level recorded in the control. The glucose concentration and superoxide dismutase enzyme activity were found to be higher in POP 2.5‐fed fish, whereas POP 10‐fed fish showed higher catalase activities compared with other groups (p < .05). Based on the result obtained, this study showed that dietary POP had no significant impact on the growth performance but has direct effects on the whole‐body lipid content, lymphocyte count, cholesterol level, alanine aminotransferase, alkaline phosphatase and antioxidant response of Clarias gariepinus.  相似文献   

8.
The current study was carried out to evaluate the effects of dietary supplementation of Coriandrum sativum seed powder (CP) and extract (CE) on some immune functions of Oreochromis niloticus, without or with exposure to the immunotoxicant metal lead (Pb). Fish (n = 300) were divided into ten groups, in triplicate. First group (Cont) was fed on the basal control diet. Second (CP20) and third (CP30) groups were fed on CP‐supplemented diets at 20 mg/kg and 30 mg/kg respectively, while fourth (CE20) and fifth (CE30) groups were fed on CE‐supplemented diets at 20 mg/kg and 30 mg/kg respectively. Fish of the sixth (Cont‐Pb), seventh (CP20‐Pb), eighth (CP30‐Pb), ninth (CE20‐Pb) and tenth (CE30‐Pb) groups were exposed to lead (Pb) at 20.2 mg/L and received same diets presented to groups; Cont, CP20, CP30, CE20 and CE30 respectively. The experiment lasted for 45 days. CP and CE dietary supplementation not only significantly enhanced the immune functions, serum lysozyme, nitric oxide, bactericidal activities and relative expression of interleukin‐1β (IL‐1β) of O. niloticus in a concentration‐dependent manner, but also ameliorated the lead‐induced immunosuppressive effects, especially in CE30 received groups. Additionally, lead‐induced fish mortality and levels of lead residues in fish musculature were significantly reduced in response to feeding on CP‐ and CE‐supplemented diets, especially in CE30. Therefore, it could be concluded that coriander dietary supplementation, especially the extract (CE) at 30 mg/kg diet, could be used to enhance the immune response of O. niloticus and to counteract the immunotoxic effects of lead exposure.  相似文献   

9.
Y. Wang  M. Li  K. Filer  Y. Xue  Q. Ai  K. Mai 《Aquaculture Nutrition》2017,23(5):1113-1120
This trial was conducted to evaluate the effects of replacing dietary fish oil with Schizochytrium meal for Pacific white shrimp (Litopenaeus vannamei) larvae (initial body weight 4.21 ± 0.10 mg). Six test microdiets were formulated using Schizochytrium meal to replace 0 g/kg, 250 g/kg, 500 g/kg, 750 g/kg, 1000 g/kg or 1500 g/kg fish oil DHA. No significant differences were observed in survival, growth, final body length and activities of digestive enzyme among shrimp fed different diets (p > .05). No significant differences were observed in C20:5n‐3 (EPA) in muscle samples (p > .05). C18:3n‐3 and C20:4n‐6 in muscle increased as Schizochytrium meal replacement level increased (p < .05). No significant differences were observed in C22:6n‐3 (DHA) and n‐3 fatty acids among shrimp fed diets that algae meal replaced 0 g/kg ‐ 1000 g/kg of fish oil. Shrimp fed diet R150 had higher DHA content than other groups and had higher n‐3 fatty acids than that of shrimp fed diets R50, R75 and R100 (p < .05). C18:2n‐6, PUFA and n‐6 fatty acids in muscle increased, while n‐3/n‐6 ratio decreased with increasing algae meal replacement level from 0 g/kg to 1000 g/kg (p < .05). In conclusion, Schizochytrium meal could replace 1500 g/kg fish oil DHA in the microdiets without negatively affecting shrimp larvae survival, growth and activities of digestive enzyme.  相似文献   

10.
A 54‐day experiment was conducted to investigate the effects of dietary supplementation of Astragalus membranaceus extract powder (AMEP) on the low temperature tolerance of genetically improved farmed tilapia, Oreochromis niloticus. The base diet was supplemented with 0.0% (NC), 0.1% (NP0.1), 0.2% (NP0.2), and 0.4% (NP0.4) AMEP. Tilapia with an initial body weight of 21.35 ± 0.17 g were fed three times a day for 14 days at 30°C and then fed for 40 days while the water temperature decreased at a rate of 0.5°C/day. The results showed that survival, final weight, and the rate of weight gain in fish in the NP0.1, NP0.2, and NP0.4 groups were higher than those of fish in the NC group. The contents of glucose, triglyceride, cholesterol, and nitric oxide in the serum of fish fed diets supplemented with AMEP were all significantly lower than those of fish in the NC group. In contrast, fish fed with AMEP‐supplemented diets had higher liver superoxide dismutase, glutathione peroxidase and catalase activities, and lower malonaldehyde content than those of fish in the NC group at 10°C. The expression of the growth‐arrest‐specific 2 gene in the liver was significantly lower in the NP0.2 and NP0.4 groups than in the NC group. The results of this study demonstrated that the optimal level of AMEP supplementation was 0.2% under the conditions used in the current study. Optimum dietary AMEP can promote growth performance and facilitate the survival of tilapia under low‐temperature stress.  相似文献   

11.
In this study, a 60‐day feeding trial was conducted to determine the effects of feed supplementating the feed of Apostichopus japonicus with peptides on its growth, energy budget, body composition and immune responses. Sea cucumbers were fed with five experimental diets supplemented with different proportions of peptides: 0 (D1), 12.5 (D2), 25 (D3), 37.5 (D4) and 50 (D5) g/kg in basal diet. Our results suggested that specific growth rate (SGR) and ratio of viscera to body wall (RVBW) of sea cucumbers fed with D4 were significantly improved. Relative to D1, ingestion rate (IR) and faeces production rate (FPR) for D3 and D4 were reduced considerably. Notably, the energy intake increased when peptide level increased from 0 to 50 g/kg. Meanwhile, the energy deposited for growth increased, and the energy loss decreased when peptide level increased from 0 to 37.5 g/kg. The sea cucumbers in D4 had the highest level of crude fat and lowest crude ash. The activities of immunoenzyme, such as SOD, CAT, T‐AOC, ACP and AKP, increased with peptide increase. Results suggested that supplementation of the feed with 25–37.5 g/kg peptides could significantly improve the growth performance, body composition and immune capacity of Apostichopus japonicus.  相似文献   

12.
The current study was conducted to evaluate the effect of dietary soy isoflavones (SI) on growth performance, antioxidant status, immune response and resistance to Aeromonas hydrophila in juvenile grass carp (Ctenopharyngodon idella). Six diets were formulated to contain 0 (control), 10, 50, 100, 500 or 1,000 mg SI per kg feed. Each diet was randomly allotted to triplicate net cages, and each net cage was stocked with 30 fish. The fish were fed one of the experimental diets to satiation twice per day for 60 days. The results showed that the WGR and DGC of the 500 mg/kg SI‐supplemented group were significantly higher than those of the non‐SI‐supplemented group (p < .05). Serum LZM and IgM activities in the SI‐supplemented groups were improved compared to the control group. SOD and GSH‐Px levels of fish fed the diet containing 500 mg/kg SI were significantly enhanced compared to those of fish fed the control diet (p < .05). Additionally, serum CAT, GSH‐Px and AKP activities in 50, 100 and 500 mg/kg SI‐supplemented groups were significantly higher than that in the control group (p < .05). The expression of most immune‐related genes (including IFN‐γ2, TNF‐α, M‐CSF2, IL‐6, IL‐12p40 and IL‐4) was significantly affected by dietary supplementation of SI. The group fed with 500 mg/kg SI had the highest 7‐day cumulative survival rate after challenge test (p < .05). The current results revealed that dietary inclusion of SI could improve the immune response and resistance against A. hydrophila and the supplementation level is suggested to be 500 mg/kg diet.  相似文献   

13.
The effects of taurine supplementation on the growth and intestinal immune function in young grass carp (Ctenopharyngodon idella) were investigated in this study. A total of 540 fish (initial average weights of 255.74 ± 0.65 g) were fed one fishmeal diet and five all‐plant protein source‐based diets with graded levels of taurine (0 to 1.98g/kg diet) for 60 days, and then challenged with Aeromonas hydrophila for 14 days. First, the results showed that the taurine supplementation improved growth (PWG, TGC, FI and FE), enteritis resistance, intestinal antimicrobial compounds (LZ, ACP, C3, C4, IgM, hepcidin, LEAP‐2A, LEAP‐2B, β‐defensin‐1 and MUC2) and attenuated intestinal inflammation in young grass carp under the all‐plant protein source‐based diet. Second, the taurine supplementation attenuated intestinal inflammation partially referring to nuclear factor kappa B (NF‐κB) and target of rapamycin (TOR) signallings. Finally, under the all‐plant protein source‐based diet, the comparable level of taurine supplementation based on growth and ability against enteritis relative to fishmeal diet was established as 0.50 g/kg diet, and the optimal levels of taurine supplementation based on thermal‐unit growth coefficient (TGC), ability against enteritis and acid phosphatase (ACP) activity were established as 0.97, 1.08 and 1.21 g/kg diet, respectively.  相似文献   

14.
A feeding trial was conducted to evaluate the potential of dietary supplementation of autolysed brewer's yeast (AY) on African catfish. The catfish (22.5 ± 1.15 g/fish, 20 fish 33 L/tank) were fed with either of diets (390 g/kg crude protein, 140 g/kg lipid) supplemented with 0, 3, 6 or 10 g/kg AY (n = 3). After 49 days of feeding, the final body weight and metabolic growth rate of the catfish fed 3 g/kg AY (3‐AY) diet were higher than those fed the control diet (p < .05). The lowest level (p < .05) of alanine transaminase was detected in the blood of the catfish fed 3‐AY diet. The mid‐intestinal histology of the catfish revealed no significant difference (p > .05) in intestinal perimeter ratio. However, an elevated (p < .05) abundance of goblet cells and intraepithelial leucocytes were found in the intestine of catfish fed 3, 6 and 10 g/kg AY diets, with the highest level of abundance recorded in the mid‐intestine of the catfish fed 3‐AY diet. The results suggest that dietary 3 g/kg autolysed brewer's yeast supplementation improves growth performance of African catfish without deleterious effect on liver functionality and gut morphology.  相似文献   

15.
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16.
Algamune? is a commercial additive produced from Euglena gracilis, providing a rich source of the β‐1,3‐glucan paramylon. Isolated kidney phagocytes of Nile tilapia were incubated with graded doses (0, 8, 16, 32, 64 and 128 μg/ml) of Algamune? and purified paramylon to gauge their ability to elicit the production of reactive oxygen species. A linear response was observed for extracellular superoxide anion for both sources but only Algamune? for intracellular superoxide anion. After corroborating the immunostimulant properties ex vivo, a feeding trial was conducted to evaluate the dietary supplementation of Algamune? (0, 100, 200, 400 and 800 mg/kg of diet) for Nile tilapia. Fish were fed for 3 weeks, after which, fish were sampled for blood and head kidney phagocytes. The remaining fish were challenged with Streptococcus iniae. Macrophage extracellular superoxide anion production was significantly elevated in fish fed diets with 200 mg of Algamune? kg?1 when compared to fish fed the basal diet. Even though the disease challenge did not show statistical differences, it is worth mentioning that fish fed intermediate doses of Algamune? had lowest numerical mortality values. Therefore, Algamune? was demonstrated to enhance some immunological responses of tilapia both in ex vivo and in vivo.  相似文献   

17.
A 10‐week growth trial was run to evaluate effects of myo‐inositol (MI) on growth performance, haematological parameters, antioxidative capacity and salinity stress tolerance of Litopenaeus vannamei. Six practical diets supplemented with graded levels of MI (designated as MI0, MI600, MI1200, MI2400, MI 3600 and MI4800 for 448.8, 974.2, 1568.0, 2810.6, 3835.5 and 4893.6 mg/kg diet, respectively) were fed to six replicate groups of L. vannamei (mean initial body weight 0.63 ± 0.00 g). The results showed that significant increment of growth performance was observed in shrimp fed MI600 diet than those fed MI1200 diet. Lipid concentration in whole body of the shrimp fed MI600 diet was significantly increased. Shrimp fed MI0 diet had lower total protein (TP) as compared to shrimp fed the MI‐supplemented diets (except MI4800 diet). In general, lower activities of antioxidant enzymes and higher malondialdehyde (MDA) content in haemolymph and hepatopancreas were recorded in shrimp fed MI0 diet, compared to those fed the MI‐supplemented diets. Reduced survival after 7‐h salinity stress was present in shrimp fed MI0 diet as compared to those fed MI4800 diet. Dietary MI requirement for glutathione peroxidase activity of L. vannamei was 2705 mg/kg diet.  相似文献   

18.
Effect of nitrite exposure on growth and immuno‐metabolic responses of Labeo rohita fed L‐tryptophan (TRP) was studied. Fish previously fed normal and elevated levels of tryptophan for 60 days were exposed to nitrite (2.0 mg L?1) for another 45 days with same feeding regime. There were four treatment groups, viz., TRP0‐N (control), TRP0+N, TRP0.75+N (0.75% supplemental tryptophan in the diet) and TRP1.5+N (1.5% supplemental tryptophan in the diet). Highest weight gain% and SGR were observed in control and lowest in TRP0+N. Dietary supplementation of elevated levels of tryptophan augmented weight gain% and SGR. Nitrite exposed groups recorded higher catalase, SOD, LDH, AST and ALT activities compared with control. However, activities reduced with additional levels of tryptophan supplementation. Nitrite exposure reduced WBC count, total protein, albumin, globulin and lysozyme activity compared with unexposed group but groups which were fed additional amounts of tryptophan restored total protein, albumin and globulin similar to TRP0‐N. In conclusion, nitrite exposure had adversely affected growth, increased activities of LDH, AST, ALT, catalase, but decreased WBC, serum protein, lysozyme and acetylcholine esterase activity of L. rohita. Normal requirement of tryptophan was unable to combat nitrite stress. However, dietary fortification with tryptophan (minimum 0.75% of diet + normal requirement) found effective in combating nitrite induced stress.  相似文献   

19.
The present study was conducted to administer a commercial multi‐strain probiotic (MP), Yilibao (Bacillus velezensis:Bacillus cereus:Lactobacillus casei = 2:2:1), in Nile tilapia, Oreochromis niloticus. In terms of aerobic Bacillus spp. counts, we produced five diets containing 0, 0.34, 1.68, 3.36 and 6.72 g/kg of MP dry product (Control, T1, T2, T3, T4). Seven hundred and fifty tilapia juveniles (13.26 ± 0.01 g) distributed into 25 tanks in five replications were fed their diet for eight weeks. Results showed that fish fed T3 and T4 diets displayed significantly higher final body weight and weight gain (p < .05). Further, all MP‐treated fish exhibited remarkably decreased plasma lipid profiles (cholesterol, triglycerides) (p < .05) and fish fed T3 and T4 diets displayed significantly higher plasma myeloperoxidase activity and complement C3 content (p < .05). Additionally, fish fed T3 and T4 diets exhibited significantly promoted total antioxidant capacity, glutathione peroxidase activity, glutathione level and declined malondialdehyde content in the plasma and/or liver (p < .05). Moreover, significantly elevated chymotrypsin activity, villus height and intraepithelial lymphocytes counts were found in fish fed T3 and T4 diets (p < .05). As fish fed T3 and T4 diets surpassed the other treatments in growth, immune–antioxidative status and gut health, the recommended dose for tilapia is 3.36–6.72 g/kg.  相似文献   

20.
An 8‐week feeding trial was conducted to determine the requirement of protein for large‐size grouper Epinephelus coioides (initial body weight: 275.07 ± 1.56 g). Six iso‐lipidic (124 g/kg) diets were formulated containing graded levels of protein (350, 400, 450, 500, 550 and 600 g/kg). Grouper was hand‐fed twice daily to apparent satiation with triplicate. The results showed that significantly high weight gain, specific growth rate and significantly low feed conversion ratio were observed in fish fed 450 g/kg protein group. High‐protein level diets significantly increased protein content and significantly decreased lipid content of fish body and muscle. Total protein and cholesterol content in serum of 600 g/kg group were significantly higher than those of 350 g/kg group. However, serum glucose and triglyceride contents of fish fed low‐protein diets were significantly higher than those of fish fed high‐protein diets. Meanwhile, liver glutamic‐pyruvic transaminase and glutamic‐oxaloacetic transaminase in high‐protein diet groups were significantly higher than those of low‐protein diet groups. The intestinal protease activity in high‐protein diet groups was significantly higher that of low‐protein diet groups, but lipase and amylase showed opposite trend. With the increasing of dietary protein level, the activities of alkaline phosphatase, superoxide dismutase and lysozyme in liver of grouper increased significantly compared with 350 g/kg group, while the activities of acid phosphatase decreased significantly. With specific growth rate as the evaluation index, the optimum dietary protein level of large‐size grouper Epinephelus coioides was 438.39 g/kg by fitting the broken‐line regression analysis.  相似文献   

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