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1.
土壤微生物生物量碳的表观周转时间测定方法   总被引:24,自引:1,他引:24       下载免费PDF全文
吴金水  肖和艾 《土壤学报》2004,41(3):401-407
土壤微生物生物量碳周转对土壤有机质和养分循环起着决定作用。本研究建立了土壤微生物生物量碳周转时间的测定方法。培养条件下 (2 5℃、10 0 %湿度 ) ,加入14 C标记葡萄糖标记土壤微生物生物量碳 ,在 10 0d培养期内 ,每隔 2 0d测定一次14 C标记微生物生物量碳 (14 C BC) ,采用一级热力学方程拟合测定期内 (2 0~ 10 0d) 14 C BC 的周转速率常数 (k) ,由此计算土壤微生物生物量碳的表观周转时间。测定的 5个土壤在培养条件下微生物生物量碳的周转时间为 93~ 4 0 0d ,根据培养温度和实际田间年平均温度推算得到田间条件下土壤微生物生物量碳的周转时间为 1 0~ 4 1a。其主要影响因子为土壤质地 ,土壤利用方式的影响较小。土壤微生物生物量碳的周转时间能较好地反映土壤微生物生物量的周转状况及其与土壤有机质的周转和积累的关系。  相似文献   

2.
利用X射线衍射法估算了福建省221个土壤样品中粘粒云母含量并讨论了与粘粒云母含量变化有关的因素。结果表明母质是影响粘粒云母含量的主要因素。海积物、河积物及页岩上发育的土壤粘粒云母含量一般较高;基性岩上发育的土壤粘粒云母含量一般较低。风化度较高的赤红壤、红壤、黄红壤和黄壤等土类的粘粒云母含量一般较低。风化度较低的C层的粘粒云母含量一般较B层和A层高,但异源母质的堆积可以使A层粘粒云母含量高于B层和C层。  相似文献   

3.
为了研究有机物质对喀斯特地区典型土壤有机碳积累与转化的影响,采集两种喀斯特典型土壤(棕色石灰土、黑色石灰土)和一种对照土壤(红壤)的表层土壤(0~15cm),设置不添加外源物质(CK)和添加14C标记的稻草(T1)的处理,进行为期100d的土壤培养试验及矿化试验。测定并分析了这3种典型土壤类型的微生物生物量碳(MBC)表观周转时间和田间周转时间、外源有机物质对土壤原有有机碳的激发效应以及CO2释放量。结果表明:MBC表观周转时间和田间周转时间依次分别为(71±2)d、(243±20)d、(254±45)d和0.29、1.0、1.04a。添加标记的14C有机物质后,土壤原有有机质的激发效应大小依次为红壤〉棕色石灰土〉黑色石灰土。在相同条件下,红壤不利于土壤有机质的累积,而棕色石灰土和黑色石灰土有利于土壤有机质的累积。  相似文献   

4.
长期施肥对红壤旱地土壤活性有机碳和酶活性的影响   总被引:18,自引:3,他引:15  
张继光  秦江涛  要文倩  周睿  张斌 《土壤》2010,42(3):364-371
以江西进贤长期肥料定位试验为平台,研究了红壤旱地不同施肥措施对土壤微生物生物量、活性有机C、C库管理指数以及土壤酶活性的影响。研究结果表明:与不施肥和单施化肥土壤相比,施有机肥处理土壤的pH、CEC、有机C、全N、全P、无机N、速效P、速效K及土壤微生物生物量均显著增加,土壤活性有机C和C库管理指数也较试前土壤和其他处理土壤明显提高,此外,土壤的转化酶、脱氢酶、脲酶和酸性磷酸酶活性也较其他处理显著增加。土壤微生物生物量、活性有机C以及4种土壤酶活性之间的相关关系显著,且它们均与土壤有机C、全N、全P、无机N、速效P等土壤养分呈显著正相关。因此,红壤旱地通过长期施用有机肥或与无机肥配施,不仅能显著提高土壤有机质的数量和质量,而且能增加土壤微生物生物量和酶活性,从而显著提高土壤肥力和土壤持续生产力。  相似文献   

5.
土壤微生物组在促进土壤养分循环、提高土壤固碳能力和维持土壤肥力等方面具有重要的作用。红壤是我国南方重要的耕地资源,但其有机质含量低、水土流失严重,且面临着土壤酸化导致的活性铁铝增加、作物生长受限和微生物活性下降等严峻问题。我国近年来基于红壤旱地长期定位试验和短期培育试验开展了很多工作,在通过调控红壤微生物组以缓解土壤酸化、增加有机质含量和提高氮磷养分有效性方面取得了突破性进展。本文综述了红壤区农田土壤微生物组结构的主要驱动因素,回顾和比较了长期施用化肥、有机肥和有机无机配施等不同施肥制度对我国南方旱地红壤微生物组结构和功能影响的研究工作,阐述了有机培肥制度对红壤微生物群落多样性的积极效应;总结了配施有机肥在提高红壤有机碳周转功能类群和氮磷代谢功能类群丰度,促进红壤有机碳分解、维持有机质稳定和提高土壤氮磷养分有效性等方面的重要作用;探讨了高多样性微生物群落中关键特殊性代谢功能对驱动微生物群落装配和维持土壤生态功能稳定的作用。最后,对我国旱地红壤微生物组未来的研究方向进行了展望,强调了可以通过改进微生物培养策略、明确微生物组不同类群的功能特征和驱动因素、充分挖掘农业微生物组资源、开发调控红壤微生物组的微生物肥料产品和高效农业管理措施来提高红壤养分循环效率、促进有机质稳定和降低铁铝活性,充分发挥微生物组在红壤耕地资源可持续利用与农业绿色发展中的作用。  相似文献   

6.
红壤坡耕地耕层土壤质量特征及障碍因素研究   总被引:3,自引:2,他引:1       下载免费PDF全文
为探究红壤坡耕地耕层质量特征及其障碍因素,通过野外调查、资料查阅及室内土壤理化性质分析等综合性研究手段,对江西红壤坡耕地耕层土壤质量统计特征、演变特征及主要障碍因素进行分析。结果表明:(1)红壤坡耕地田面坡度主要分布在2~16°之间,耕层平均厚度13.40 cm,有效土层厚度平均88.30 cm,土壤容重平均为1.17 g/cm~3;耕层土壤有机质平均含量19.37 g/kg,土壤pH值平均5.36。(2)红壤坡耕地耕层质量近20年有明显提高,田面坡度从6°降至4°,耕层厚度从13.68 cm增至16.42 cm;耕层土壤有机质含量24.63 g/kg,提高33.93%,全氮、有效磷和速效钾含量分别增加10.53%、230.98%、44.18%。(3)红壤坡耕地低产耕层土壤质量的主要障碍因素是土壤养分贫瘠化、粘重化和酸化;花生和木薯低产耕层的土壤容重和粘粒含量均大于高产耕层,而土壤孔隙度、田间持水量、有机质含量及pH值均小于高产耕层,表明高产坡耕地耕层土壤质量优于低产坡耕地。研究结果可为江西红壤坡耕地耕层质量改善和合理耕层构建提供科学参考。  相似文献   

7.
为探究侵蚀退化红壤马尾松林恢复过程中林下芒萁对土壤微生物生物量碳氮月动态及其周转的影响,以不同恢复年限的马尾松林为研究对象,对比分析马尾松林恢复过程中林下保留芒萁、去除芒萁处理和林下裸地土壤中12个月的土壤微生物生物量碳(MBC)和微生物量氮(MBN)含量及其周转速率、周转时间和流通量,并分析其与土壤理化性状的关系。结果表明:(1)保留芒萁覆盖处理的MBC和MBN平均含量分布比林下裸地提高26.99%~277.31%和13.54%~173.39%,而去除芒萁处理分布比保留芒萁处理降低12.29%~27.01%和5.02%~28.45%,差异均随恢复年限呈先降低后增加的趋势。(2)所有处理的土壤微生物量碳氮季节动态均表现为春夏季较高,秋冬季较低的趋势,进入生长季前的土壤微生物量碳氮含量更能反映该地区的平均水平。(3)在退化马尾松林恢复过程中,芒萁覆盖降低土壤微生物生物量碳氮周转速率,增加周转时间,提高土壤微生物生物量碳氮含量和流通量,促进土壤有机质的积累和养分释放。相关分析和逐步回归分析表明,MBC、MBN流通量分别与DOC、DON呈显著正相关,周转速率分别与铵态氮(NH4+-N)和TN呈显著负相关,表明土壤碳和氮及其有效性是影响土壤微生物量周转的关键因素。  相似文献   

8.
根据所收集的土壤理化分析资料,探讨了广东坡地红壤类型颗粒组成对土壤理化性质的影响,研究结果表明,土壤〈0.01mm物理性粘粒或是〈0.001mm粘粒含量与土壤结构系数,〈0.005mm孔隙度,土壤持水量,土壤阳高子代换量,土壤有机质,土壤N和P含量均呈显著正相关,〈0.01mm颗粒含量与土壤有效持水量呈显著指数相关,由此可见,土壤颗粒组成是影响土壤肥力的重要因素。  相似文献   

9.
为研究长期有机无机肥配施对红壤性水稻田作物产量、土壤微生物生物量及有机碳分子结构的影响,以始于1984年的江西红壤性水稻田长期定位试验为平台,选取的试验处理包括:不施肥(CK)、单施化肥(NPK)和等养分条件下70%化肥配施30%有机肥(NPKM1)、50%化肥配施50%有机肥(NPKM2)、30%化肥配施70%有机肥(NPKM3),采用固体~(13)C核磁共振测定了土壤有机碳组分含量,分析了土壤化学指标和土壤微生物生物量碳(Microbial biomass carbon,MBC)和微生物生物量氮(Microbial biomass nitrogen,MBN)。结果表明,连续34年的不同施肥处理显著影响了水稻产量、土壤微生物生物量及土壤有机碳(SOC)分子结构。与NPK处理相比,有机肥配施(NPKM1、NPKM2、NPKM3)提高了水稻产量,增幅为6.5%~7.7%(P0.05),中低有机肥配施比例(30%和50%)稳产效果更优。长期单施化肥使土壤严重酸化,而配施有机肥可减缓土壤酸化。长期施肥处理MBC和MBN较CK处理分别显著提高17.0%~71.1%和104.1%~267.0%,但MBC/MBN下降,有机无机肥配施处理较NPK处理提高了微生物熵。长期单施化肥主要提高了烷基碳的相对含量,而配施有机肥同时提高烷基碳和烷氧碳(甲氧基/含氮烷基碳)含量,有利于土壤活性有机质累积。Pearson相关性分析表明土壤微生物生物量与SOC、氮磷养分指标及甲氧基/含氮烷基碳呈显著或极显著正相关,与芳基碳和羧基碳呈显著负相关。冗余分析显示SOC、有效磷、速效钾及烷基碳等对水稻产量的影响较大。研究表明,在供试条件下,长期实行中低比例有机肥配施化肥有利于提高土壤养分和土壤微生物生物量,并改善土壤有机质结构,是维持作物高产和提升土壤质量的有效施肥措施。  相似文献   

10.
红壤地区地形位置和利用方式对土壤物理性质的影响   总被引:15,自引:2,他引:15  
对不同地形部位和不同利用方式下的低丘红壤进行了采样和分析,结果表明地形变化和利用方式对土壤的理化性质和水分特性有明显的影响。对受人为扰动较小的林地和茶园而言,随地形位置的降低,土壤粘粒含量降低,有机质含量、土壤水稳性团聚体数量、土壤通气性均明显增加,而对受人为耕作影响较大的旱地和桔园,其养分含量、土壤结构性质变化没有明显规律。红壤的持水和供水性质也受到影响,坡顶的持水量比坡中和坡底要大,同时由于毛管孔隙数量比例小,土壤难以保证连续快速的水分供应是红壤作物易旱的重要原因。  相似文献   

11.
红壤微生物量氮的周转期及其研究意义   总被引:34,自引:4,他引:30  
本文采用^15N标记底物法测定了三种不同肥力水平的红壤中微生物量氮周转 速率,结果表明三种红壤中微生物量氮的周期转分别为63天,89在和251天。  相似文献   

12.
四种农业土壤上生物炭-土壤的交互效应   总被引:1,自引:0,他引:1  
Soils in south-western Australia are highly weathered and deficient in nutrients for agricultural production. Addition of biochar has been suggested as a mean of improving soil C storage, texture and nutrient retention of these soils.~Clay amendment in sandy soils in this region is a management practice used to improve soil conditions, including water repellence.~In this study a woody biochar (Simcoa biochar) was characterised using scanning electron microscopy before, and four weeks after, it was incorporated into each of four soils differing in clay content and organic matter. Scanning electron microscopy of Simcoa biochar after incubation in soil showed different degrees of attachment of soil particles to the biochar surfaces after 28 d. In addition, the effects of three biochars, Simcoa biochar, activated biochar and Wundowie biochar, on soil microbial biomass C and soil respiration were investigated in a short-term incubation experiment. It was hypothesised that all three biochars would have greater potential to increase soil microbial activity in the soil that had higher organic matter and clay. After 28-d incubation in soil, all three biochars had led to a higher microbial biomass C in the clayey soil, but prior to this time, less marked differences were observed in microbial biomass C among the four soils following biochar application.  相似文献   

13.
Based on a literature review including 201 surface soils from wet, mild, mid-latitude climates and 290 soils from the Lower Saxony soil monitoring programme (Germany), we investigated the relationship between soil clay content and soil organic matter turnover. The relationship was then used to evaluate the clay modifier for microbial decomposition in the organic matter module of the soil-plant-atmosphere model DAISY. A positive relationship was found between soil clay content and soil microbial biomass (SMB) C. Furthermore, a negative relationship was found between soil clay content and metabolic quotient (qCO2) as an indicator of specific microbial activity. Both findings support the hypothesis of a clay dependent capacity of soils to protect microbial biomass. Under the differing conditions of practical agriculture and forestry, no or only very weak relationships were found between soil clay content and non-living soil organic matter C (humus C). It is concluded that the stabilising effect of clay is much stronger for SMB than for humus. This is in contrast to the DAISY clay modifier assuming the same negative relationship between soil clay content, on the one hand, and turnover of SMB and turnover of soil humus on the other. There is a positive relationship between SMB and microbial decomposition activity under steady-state conditions (microbial growth≈microbial death). The original concept of a biomass-independent simulation of organic matter turnover in the DAISY model must therefore be rejected. In addition to the original modifiers of organic matter turnover, a modifier based on the pool size of decomposing organisms is suggested. Priming effects can be simulated by applying this modifier. When using this approach, the original modifiers are related to specific microbial activity. The DAISY clay modifier is a useful approximation of the relationship between the metabolic quotient (qCO2) as an indicator of specific microbial activity and soil clay content.  相似文献   

14.
Summary Microbial biomass C and N respond rapidly to changes in tillage and soil management. The ratio of biomass C to total organic C and the ratio of mineral N flush to total N were determined in the surface layer (0–5 cm) of low-clay (8–10%), fine sandy loam, Podzolic soils subjected to a range of reduced tillage (direct drilling, chisel ploughing, shallow tillage) experiments of 3–5 years' duration. Organic matter dynamics in the tillage experiments were compared to long-term conditions in several grassland sites established on the same soil type for 10–40 years. Microbial biomass C levels in the grassland soils, reduced tillage, and mouldboard ploughing treatments were 561, 250, and 155 g g-1 soil, respectively. In all the systems, microbial biomass C was related to organic C (r=0.86), while the mineral N flush was related to total N (r=0.84). The average proportion of organic C in the biomass of the reduced tillage soils (1.2) was higher than in the ploughed soils (0.8) but similar to that in the grassland soils (1.3). Reduced tillage increased the average ratio of mineral N flush to total soil N to 1.9, compared to 1.3 in the ploughed soils. The same ratio was 1.8 in the grassland soils. Regression analysis of microbial biomass C and percent organic C in the microbial biomass showed a steeper slope for the tillage soils than the grassland sites, indicating that reduced tillage increased the microbial biomass level per unit soil organic C. The proportion of organic matter in the microbial biomass suggests a shift in organic matter equilibrium in the reduced tillage soils towards a rapid, tillage-induced, accumulation of organic matter in the surface layer.  相似文献   

15.
In order to investigate the effect of soil water and texture on C and N mineralisation of applied organic matter, sheep manure was sandwiched between two halves of intact soil cores and incubated at 20°C. The soils contained 10.8% (L1), 22.4% (L3) and 33.7% (L5) clay, respectively, and were drained to seven different matric potentials in the range -15 to -1,500 hPa. Evolution of CO2-C was determined during 4 weeks of incubation. Contents of NO3--N, 15N and microbial biomass N were determined at the end of the incubation. The net release of CO2-C from the manure (estimated as the difference between soils with and without manure) and the total CO2-C evolution from soils with manure was not related to soil water content. Most CO2-C evolved from manure-amended soils in the least clayey L1 soil. The manure caused immobilisation of soil NO3--N but the soil matric potential had no major effects on the net NO3--N production. Less than 1% of the manure 15N was found as NO3--N at the end of the incubation. When unamended, the sandy L1 soil held the least N in microbial biomass but the largest increases in biomass N caused by manure application were found in this soil. Despite the higher increases in microbial biomass N in the L1 soil, the total content of microbial biomass N in soils with manure application peaked in the most clayey soil (L5). The recovery of manure 15N at the end of the incubation ranged from 89% to 102%. The variation in 15N recovery was not related to soil clay content nor to soil matric potential. The experimental set-up was designed to mimic field conditions where manure is left as a discrete layer surrounded by structurally intact soil. In this situation the soil clay content and the soil water level appeared to have little influence on the C and N turnover in the manure layer.  相似文献   

16.
Vicia faba, in a pot experiment with sandy and clayey soils under greenhouse conditions, was checked for growth response to different amendments with coal alkaline fly ash or cocomposted fly ash mixed with lignocellulosic residues. Soil microbial populations, pH and electrical conductivity as well as heavy metal uptake by plants were monitored. At rates of five and ten percent (on a dry matter basis) in both soils, neither fly ash alone nor cocomposted fly ash exerted any negative effect. Plant biomass production was not influenced in either clayey or sandy soil. Alkaline fly ash did not promote microbial growth when applied alone to the soils. However, cocomposted fly ash generally increased bacterial and actinomycetes counts in both soils. Fungi were not affected by ash. Due to the increase of soil pH by alkaline fly ash or cocomposted fly ash, plant uptake of heavy metals was depressed in the sandy soil. Heavy metal mobility did not cause change in the clayey soil where a high buffering capacity mitigated the effects of fly ash amendments.  相似文献   

17.
The effects of adding P and of drying and rewetting were studied in two acid forest soils from southeast Australia. The soils were a yellow podzolic with a low soil organic matter content (3.75% C) and a red earth with a high organic matter content (13.5% C). C and N mineralization and microbial C and N contents were investigated in a laboratory incubation for 151 days. Microbial C and N were estimated by a hexanol fumigation-extraction technique. Microbial C was also determined by substrate-induced respiration combined with a selective inhibition technique to separate the fungal and the bacterial biomass. The results obtained by the selective inhibition technique were not conclusive. Adding P to the soil and drying and rewetting the soil reduced microbial N. This effect was more pronounced in rapidly and frequently dried soils. Microbial C was generally less affected by these treatments. Compared with the control, the addition of P caused a reduction in respiration in the red earth (-13%) but an increase in the yellow podzolic soil (+12%). In the red earth net N mineralization was highest following the addition of P. In the yellow podzolic soil highest N mineralization rates were obtained when the soil was subjected to drying and rewetting cycles. In both soils increased N mineralization was associated with a decrease in microbial N, indicating that the mineralized N was of microbial origin. Nitrification decreased with rapid drying and rewetting. The addition of P promoted heterotrophic nitrification in both soils.  相似文献   

18.
Addition of clay-rich subsoil to sandy soil results in heterogeneous soil with clay peds(2-mm) or finely ground( 2 mm) clay soil(FG), which may affect the nutrient availability. The aim of this study was to assess the effect of clay soil particle size(FG or peds)and properties on nutrient availability and organic C binding in sandy soil after addition of residues with low(young kikuyu grass,KG) or high(faba bean, FB) C/N ratio. Two clay soils with high and low smectite percentage, clay and exchangeable Fe and Al were added to a sandy soil at a rate of 20%(weight/weight) either as FG or peds. Over 45 d, available N and P as well as microbial biomass N and P concentrations and cumulative respiration were greater in soils with residues of KG than FB. For soils with KG residues,clay addition increased available N and initial microbial biomass C and N concentrations, but decreased cumulative respiration and P availability compared to sandy soil without clay. Differences in measured parameters between clay type and size were inconsistent and varied with time except the increase in total organic C in the 53 μm fraction during the experiment, which was greater for soils with FG than with peds. We concluded that the high exchangeable Fe and Al concentrations in the low-smectite clay soil can compensate a lower clay concentration and proportion of smectite with respect to binding of organic matter and nutrients.  相似文献   

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