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1.
Brown planthopper (BPH) is the most devastating insect pest in rice‐growing areas. Information on availability of BPH resistance alleles and their sources enhances BPH‐resistant breeding programmes. In this study, 260 highly diversified rice cultivars or breeding lines were screened for the presence of five major BPH resistance genes (Bph10, Bph13, Bph18, Bph20 and Bph21) using gene‐specific markers. The analysis revealed that 137 of the 260 cultivars possess at least one BPH resistance gene. Bph10 was predominant while Bph20 was the least distributed. Moreover, two and three different resistance gene combinations were found in the cultivars. Molecular markers play an important role in molecular breeding programmes. A tightly linked PCR‐based co‐dominant Bph18 marker was developed, which is cost effective and time effective and simpler than available Bph18 CAPS marker (7312.T4A). We strongly believe that the identified BPH‐resistant cultivars can be used as alternative resistance gene sources and also as resource for novel BPH resistance genes. The developed Bph18 marker will be highly useful in molecular breeding applications of BPH‐resistant breeding programmes.  相似文献   

2.
To improve brown planthopper (Nilaparvata lugens Stål; BPH) resistance of an elite indica cultivar of South China, Hemeizhan (HMZ), we applied marker‐assisted backcross (MABC) to incorporate three BPH‐resistance genes (Bph3, Bph14 and Bph15) into the genetic background of HMZ. In the third backcross (BC3) generation, we obtained near‐isogenic lines (Bph3‐NIL, Bph14‐NIL, Bph15‐NIL and Bph14 + Bph15‐NIL) with more than 96% recovery of recurrent parent genome, and pyramided lines (Bph3 + Bph14‐PYL, Bph3 + Bph15‐PYL and Bph3 + Bph14 + Bph15‐PYL) with more than 89% recovery of recurrent parent genome. These lines showed stronger resistance against BPH than HMZ at seedling and booting stages. The rank of resistance gene effect was Bph3 + Bph14 + Bph15 ≥ Bph3 + Bph15 ≥ Bph3 +Bph14 ≥ Bph14 + Bph15 ≥ Bph3 ≥ Bph15 ≥ Bph14 > none. Compared with HMZ, only Bph3 + Bph14 + Bph15‐PYL had a significant difference in yield per plant, and the lines carrying Bph3 had higher amylose contents, indicating that Bph3 was tightly linked to Wxa allele. These improved lines are good intermediate sources of broad‐spectrum and durable BPH resistance to improve other indica cultivars. Our results demonstrate that MABC is a very efficient approach to improve BPH resistance of elite rice cultivar.  相似文献   

3.
We have constructed a linkage map of the rice brown planthopper (BPH)resistance gene, Bph1. RFLP and AFLP markers were selected by thebulked segregant analysis and used in the mapping study of 262 F2sthat were derived from a cross of `Tsukushibare', a susceptible japonica cultivar, and `Norin-PL3', an authentic japonicaBph1-introgression line. Twenty markers were mapped within a 28.9-cMregion containing the Bph1 locus on the long arm of rice chromosome12. Combining the result of segregation analysis of BPH resistance by themass seedling test and that of the markers, the Bph1 locus wasmapped within a 5.8-cM region between two flanking markers. The closestAFLP markers, em5814N and em2802N, was at 2.7 cM proximal to theBph1 locus. Together with the previously constructed high-resolutionmap of bph2 locating the locus at ca. 10 cM proximal to the Bph1 locus, this improved version of the linkage map would facilitatepyramiding these two important BPH resistance genes.  相似文献   

4.
Rice leaf folder, stem borer and brown planthopper (BPH) are the most devastating rice insect pests. Developing and planting insect-resistant rice varieties is the most economical and effective measure for controlling these pests. BPH can be controlled with native BPH-resistance genes in rice, while at present rice leaf folder and stem borer can only be controlled through planting transgenic Bt rice. In this study, the breeding of a new restorer line KR022 possessing stacked BPH-resistance genes Bph14 and Bph15, Bt gene cry1C and glufosinate-resistance gene bar, is reported for the first time. A rice restorer line R022 with BPH-resistance genes Bph14 and Bph15 was used as a recurrent parent to cross with the transgenic rice T1C-19 of cry1C and bar genes during the breeding process. The restorer line KR022 was developed from the backcross populations of R022 and T1C-19 through molecular marker-assisted selection and glufosinate-resistance selection. The cry1C and bar genes were found to integrate on chromosome 11 of KR022, and the genome recovery of KR022 was up to 95.8 % of the R022 genome. The quantification of Cry1C protein expression showed that it was expressed at different levels in the leaf, stem, panicle, endosperm, and root of KR022 and its hybrid rice. The insect-resistance evaluation indicated that KR022 and its hybrid rice had good resistance to rice leaf folder and stem borer, both in laboratory settings and in the field. Furthermore, they exhibited increased resistance to BPH at both the seedling and mature stage. The field trial showed there was no significant difference in key agronomic traits between KR022 and its recurrent parent R022, and four hybrids from KR022 yield much higher than the control II-You 838. Moreover, KR022 and its hybrid rice were found to have resistance to the herbicide glufosinate. These results demonstrate that KR022 is effective as a rice restorer line for the breeding of “green super rice”, possessing multiple tolerances to rice BPH, stem borer, leaf folder and glufosinate.  相似文献   

5.
水稻抗褐飞虱基因及其育种应用研究进展   总被引：4，自引：1，他引：3  
褐飞虱是危害水稻生产最重要的害虫之一。遏制水稻褐飞虱为害的最安全、有效的措施既利用水稻自身的抗虫性。迄今为止,已经于栽培稻和野生稻中成功鉴定出30个水稻抗褐飞虱基因,其中26个主效抗性基因已被定位,显性基因Bph14已成功克隆,但仅少量主效抗性基因被育种家利用并培育出抗性品种。对褐飞虱的生物型和抗性机理、水稻抗褐飞虱的遗传基础及育种应用研究进行了综述,并就今后的抗性育种研究趋势展开了讨论。  相似文献   

6.
培育水稻恢复系抗稻褐飞虱基因导入系和聚合系   总被引：1，自引：0，他引：1  
稻褐飞虱是水稻的主要虫害之一,培育优良的抗性基因聚合系对于防治稻褐飞虱具有重要的意义.本研究通过回交、分子标记辅助选择和接虫鉴定三者相结合的办法,将抗稻褐飞虱基因Bph3和Bph24(t)分别导入主栽杂交水稻恢复系广恢998、9311、R15、明恢63、R29中,最终获得遗传稳定的Bph3导入系32份和Bph24(t)...  相似文献   

7.
Brown planthopper (BPH) is the most damaging rice pest globally. Resistant varieties are the most effective and environmental strategy for protecting the rice crop from BPH. Functional markers (FMs) designed from polymorphic sites within gene sequences affecting phenotypic variation are highly efficient when used for marker assisted selection (MAS). Bph14 is the first and only cloned insect resistance gene so far in rice. Compared to the sequences of its non-effective alleles there are a number SNP differences. In this study, the method of allele-specific amplification (ASA) was adopted to design a simple, co-dominant, functional marker Bph14P/N for Bph14. Bph14P/N was combined with two specific dominant markers: one, named Bph14P, targets the promoter region of Bph14 and amplifies 566 bp fragments; and the other, Bph14N, targets the LRR region of bph14 and amplifies 345 bp fragments. Specificity and applicability of the functional marker system were verified in two breeding populations and a Chinese mini core collection of Oryza sativa. We recommend the use of this simple, low-cost marker system in routine genotyping for Bph14 in breeding populations.  相似文献   

8.
褐飞虱是水稻的主要害虫之一,利用水稻抗褐飞虱基因培育抗虫品种是目前公认最经济有效、环境友好的策略。本研究利用水稻功能基因组已克隆的抗褐飞虱基因,通过分子标记辅助选择和常规回交育种相结合的方法,将抗褐飞虱基因Bph6、Bph9、Bph14和Bph15单独和聚合导入到节水抗旱稻恢复系旱恢3号,获得了一系列含有单基因、双基因、三基因和四基因的改良系。采用标准苗期集团筛选法进行褐飞虱抗性鉴定,评价这些基因在旱恢3号背景下的效应及相互作用。表明单基因改良系中, Bph9的抗性最强,且Bph9 Bph6 Bph15 Bph14;在聚合改良系中,抗性均优于单基因改良系,四基因聚合改良系的抗性最强,不同基因型组合的抗性效应是Bph6+Bph9+Bph14+Bph15Bph6+Bph9 Bph6+Bph9+Bph14 Bph6+Bph9+Bph15 Bph6+Bph14+Bph15 Bph9+Bph14+Bph15 Bph14+Bph15。在自然条件下,改良系与旱恢3号在株高、有效穗和千粒重等农艺性状上差异不显著,其他性状与旱恢3号相仿或略差。本试验表明单独和聚合导入Bph6、Bph9、Bph14和Bph15基因能显著提高节水抗旱稻恢复系的褐飞虱抗性,这4个基因的加性效应明显,可为今后节水抗旱稻抗褐飞虱育种提供理论依据和材料基础。  相似文献   

9.
The rice brown planthopper (BPH) Nilaparvata lugens (Stål) is one of the major pests of rice across Asia. Host-plant resistance is the most ecologically acceptable means to manage this pest. A rice breeding line RP2068-18-3-5 (RP2068) derived from the land race Velluthacheera is reported to be resistant to BPH populations across India. We identified a new R gene [Bph33(t)] in this line using advanced generation RILs derived from TN1 × RP2068 cross through phenotyping at two locations and linkage analysis with 99 polymorphic SSR markers. QTL analysis through IciMapping identified at least two major QTL on chromosome 1 influencing seedling damage score in seed box screening, honey dew excretion by adults and nymphal survival. Since no BPH R gene has been reported on chromosome 1, we designate this locus as a new gene Bph33(t) which accounted for over 20% of phenotypic variance. Scanning the region for candidate gene suggested two likely candidates a leucine rich repeat (LRR) gene and a heat shock protein (HSP) coding gene. Expression profiling of the two genes in the two contrasting parents and RILs showed induction of the HSP gene (LOC_Os01g42190.1) at 6 h after infestation while LRR gene did not show such induction. It is likely that the HSP represented Bph33(t).  相似文献   

10.
标记辅助培育水稻抗稻褐飞虱和稻白叶枯病基因聚合系   总被引：5，自引：3，他引：2  
稻白叶枯病和稻褐飞虱是水稻的主要病虫害,利用优良的抗性基因培育抗性基因聚合系对于防治稻白叶枯病和稻褐飞虱具有重要意义。本研究通过回交与分子标记辅助选择相结合的方法,将抗稻白叶枯病基因Xa23和抗稻褐飞虱基因Bph3分别导入主栽杂交水稻品种的保持系先B、天B、盟B、龙特甫B和桂B中。结果表明,获得稳定的单抗性基因导入系1436份和双抗性基因聚合系144份。人工接种鉴定结果显示,Xa23导入系和Xa23Bph3聚合系对抗稻白叶枯水平达到中抗和接近高抗的水平(1.1～3.0级),Bph3导入系和Xa23Bph3聚合系抗褐飞虱水平达中抗到抗的级别(3.2～4.0级)。农艺性状分析显示多数抗性基因聚合系的株高、剑叶长宽度、每穗粒数、结实率、千粒重等主要农艺性状与受体差异不显著,只有少数聚合系有1～2个性状的差异。SSR标记分析表明抗性基因聚合系的遗传背景回复率达89.6%～97.8%,等位性位点纯合度达95.6%～99.9%。初步证明本研究已成功地获得抗稻白叶枯病和稻褐飞虱基因的聚合系,这些抗性改良保持系具有良好的应用前景,并为建立完善的分子设计育种平台提供了重要育种材料基础。  相似文献   

11.
‘大粒香’是著名的香稻品种之一,但对稻瘟病敏感的缺点限制了其推广。本研究利用分子标记YY5-YY8、Bph14P/Bph14N、MS5、Pibdom、Pi-ta、pTA248、Sub1-1,从课题组选育的129株‘大粒香’改良系F4代中筛选同时聚合香味基因badh2,抗褐飞虱基因Bph14和Bph15,抗稻瘟病基因Pita和Pib,抗白叶枯病基因Xa21及耐涝基因Sub1的单株,并从中选择农艺性状较好的单株进行对应基因的表型鉴定,以期获得具有多种抗性的香稻育种新材料。通过PCR技术对改良系F4代的badh2、Bph14、Bph15、Pita、Pib、Xa21和Sub1基因进行分子标记检测,从129个单株中筛选出同时聚合以上7个基因的植株30株,从中选择农艺性状较好的单株17C1389-4-4W进行表型鉴定。咀嚼实验和KOH浸泡-嗅闻实验结果表明17C1389-4-4W具有香味,褐飞虱接种实验结果表明17C1389-4-4W抗褐飞虱级别为3级,稻瘟病菌株Gally接种实验结果表明17C1389-4-4W抗稻瘟病级别为1级,白叶枯菌株PXO86接种试验结果表明17C1389-4-4W抗白叶枯病级别为1级,苗期淹涝实验结果显示17C1389-4-4W耐涝性显著强于亲本。大粒香改良系17C1389-4-4W聚合了多达6个抗性基因,将在多抗香稻育种中发挥重要作用。  相似文献   

12.
东乡野生稻抗褐飞虱QTL分析   总被引：5，自引：0，他引：5  
野生稻是水稻抗褐飞虱基因的重要种质资源。应用东乡野生稻与栽培稻协青早B构建的2套材料,开展水稻抗褐飞虱基因鉴定研究。先以协青早B//协青早B/东乡野生稻BC1F5群体为材料,应用褐飞虱田间种群进行抗虫鉴定,检测到2个抗褐飞虱QTL,其中,qBph2位于水稻第2染色体RM29–RG157区间,东乡野生稻等位基因可降低死苗率22.2%;qBph7位于第7染色体RM11–RM234区间,东乡野生稻等位基因可降低死苗率43.7%。进一步以协青早B为轮回亲本,构建了BC3F3群体,应用褐飞虱生物型I、II和III进行抗虫鉴定,QTL分析表明qBph2抗褐飞虱生物型I和II,qBph7抗褐飞虱生物型I和III。这2个QTL对培育抗褐飞虱水稻品种具有重要应用价值。  相似文献   

13.
稻褐飞虱(BPH,NilaparvatalugensSt#l)是中国和世界稻区最严重的水稻害虫之一,发现和利用新抗性基因和进行抗性种质创新对于稻褐飞虱抗性品种的培育具有重要的意义。本研究进行了1200多份普通野生稻(OryzarufipogonGriff)种质对多种BPH生物型的抗性鉴定,获得了30份抗性资源,其中6份对分布于世界主要稻区的稻褐飞虱生物型1和2、孟加拉、湄公河(越南)、九龙江(越南)、潘特纳加(印度)等6种生物型或其中5种具有广谱高抗性。遗传分析证明了这些种质对BPH生物型2和九龙江生物型的抗性受2对重复作用隐性基因控制,对潘特纳加生物型的抗性受1对隐性基因控制,表明抗源对不同褐飞虱生物型具有不同的遗传机制。普通野生稻材料2183存在的2对隐性基因很可能是新发现的基因,因为在这些染色体区域还没报道有BPH抗性基因,这两对基因暂命名为bph18(t)和bph19(t)。研究总共培育出143份抗性创新种质和6份抗性品系或高产优质杂交水稻组合,这些优良的抗性创新种质为培育抗性新品种建立了坚实的基础。  相似文献   

14.
利用分子标记辅助选择和田间鉴定选择相结合的方法,将抗白叶枯病基因Xa23和抗褐飞虱基因Bph18(t)导入明恢86、蜀恢527和浙恢7954等3个骨干中籼恢复系,获得带有Xa23抗性基因纯合的改良恢复系明恢86-Xa23、蜀恢527-Xa23、浙恢7954-Xa23和携有抗褐飞虱Bph18(t)基因的蜀恢527-Bph18(t)、浙恢7954-Bph18(t),并从蜀恢527/IRBB23 F1和浙恢7954/IR65482 F1复交后代中选育出的带有Xa23和Bph18(t)的双基因聚合系浙蜀-Xa23-Bph18(t)。明恢86-Xa23、蜀恢527-Xa23、浙恢7954-Xa23和浙蜀-Xa23-Bph18(t)对中国和菲律宾的17个白叶枯病菌均表现高抗,蜀恢527-Bph18(t)、浙恢7954-Bph18(t)和浙蜀-Xa23-Bph18(t)对褐飞虱的抗性也达到中抗以上水平。抗性改良恢复系及其与不育系II-32A、沪旱11A的测交种在不接种白叶枯病菌条件下的产量和结实率与原来的恢复系及相应杂交种相仿,但在接种条件下带有Xa23基因的恢复系及测交种的结实率和产量明显优于原来的恢复系及相应杂交种。研究表明,抗性基因Xa23在不同恢复系背景下的抗性表达完全,对恢复系白叶枯病改良的效果明显,而抗性基因Bph18(t)对褐飞虱的改良效果与遗传背景有关。对分子标记回交和复交改良恢复系的抗病虫性进行了讨论。  相似文献   

15.
Asian rice gall midge (Orseolia oryzae) is a major pest across much of south and southeast Asia. This pest is genetically diverse and many gall midge biotypes are known to exist in each country. During the last three decades, host plant resistance has proved to be the most effective mechanism of controlling the Asian rice gall midge. Seven genes conditioning resistance to gall midge larvae have been identified in rice (Oryza sativa) and are being used in cultivar improvement programs. However, some of these genes are rendered ineffective by new gall midge biotypes. Increased understanding of genetics, inheritance, allelic relationships and linkage is necessary to maximise the durability of major gene resistance by the pyramiding of these genes. The two genes, Gm-2 and Gm-6(t), are known to confer resistance against a number of biotypes in India and China, respectively. An F3 population derived from a cross between Duokang #1 (donor of Gm-6(t)) and Phalguna (donor of Gm-2) was screened against Chinese gall midge biotype 4 at Guangdong, China, and Indian gall midge biotype 1 at Raipur, India. At each location, separately,a single gene governed resistance. The parallel segregation of 417 F3progenies for both biotypes at two locations revealed that recombination had occurred between the two genes, establishing that the two genes are not allelic. However, the two genes Gm-2 and Gm-6(t), were found to be linked with a distance of ∼16.3 cM. A number of lines homozygous at one locus and segregating for the other locus were identified and selected. These lines were selfed to obtain lines homozygous for the favourable alleles at both loci (two locus pyramids). This is the first report on use of conventional host-pest interaction method for pyramiding two closely located Gm-resistance loci of dissimilar effects. The implications of deployment of these pyramids within and across country borders, with reference to the prevailing gall midge populations are discussed. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

16.
保持系川香29B是籼型三系细胞胞质雄性不育保持系,具有配合力高、米质优良、开花习性好且有香味等特点,本研究用我室在前期利用川香29B培育华1971B (Pi1/Pi2)的基础之上,再以华2048B (Bph14/Bph15/Ri1/Pi2)为Bph14、Bph15供体亲本,利用分子标记辅助选择和花药培养相结合的方法对川...  相似文献   

17.
C. C. Su    J. Wan    H. Q. Zhai    C. M. Wang    L. H. Sun    H. Yasui  A. Yoshimura 《Plant Breeding》2005,124(1):93-95
The brown planthopper (BPH) is one of the most destructive insect pests of rice. Resistant varieties have proved to be one of the most economic and effective measures for BPH management. In this study, an indica rice ‘DV85’ showed resistance to biotype 2 of BPH by bulked seedling test, and a recombinant inbred line (RIL) population derived from a cross between a susceptible rice ‘Kinmaze’ and ‘DV85’ was phenotyped to map genetic factors conferring BPH resistance in ‘DV85′. Composite interval mapping revealed that one quantitative trait locus (QTL) with a LOD score of 10.1 was detected between XNpb202 and C1172 on chromosome 11. This QTL was designated as Qbph11. Qbph11 explained 68.4% of the phenotypic variance of BPH resistance in this population. The allele from the resistant parent ‘DV85’ at Qbph11 reduced the damage caused by BPH feeding and would be very useful in breeding resistant rice varieties via marker‐assisted selection.  相似文献   

18.
G. S. Sidhu  G. S. Khush 《Euphytica》1979,28(2):233-237
Summary Two-way classification of 400 F3 families from the rice cross IR2153-159-1 x Babawee for plant stature and for resistance to brown planthopper, green leafhopper, and bacterial blight indicated that Glh 3 (dominant gene for resistance to green leafhopper) and bph 4 (recessive gene for resistance to brown planthopper) are linked with a map distance of 34 units. The bph 4 gene also appears to be linked with sd 1 (recessive gene for semidwarf stature) although the linkage is less strong. However, bph 4 and Xa 4 (dominant gene for bacterial blight resistance) are inherited independently of each other. No segregation for susceptibility was observed among F3 families of crosses between varieties having Bph 3 and bph 4 genes for resistance to brown planthopper. Apparently, Bph 3 and bph 4 are either allelic or closely linked.  相似文献   

19.
Bacterial blight (BB) is the most economically damaging disease of rice in Asia and other parts of the world. In this study, a multiplex PCR genotyping method was developed to simultaneously identify genotypes of five BB resistance genes, Xa4, xa5, Xa7, xa13 and Xa21. The resistance R alleles were amplified using five functional markers (FMs) to generate amplicons of 217, 103, 179, 381 and 595 bp in IRBB66. Amplicons of 198, 107, 87, 391 and 467 bp corresponded to susceptible alleles in Taiwanese japonica rice cultivars. In backcross breeding programmes, the multiplex PCR assay was integrated into selection from a population using BB resistance donor IRBB66 crossed to rice cultivar ‘Tainung82’. Two plants with homozygosity for Xa4, xa5, Xa7, xa13 and Xa21 were selected from 1100 BC2F2 plants. In addition, the five BB resistance genes were also accurately identified in F2 populations. This multiplex PCR method provides a rapid and efficient method for detecting various BB resistance genes, which will assist in pyramiding genes to improve durability of BB resistance in Taiwanese elite rice cultivars.  相似文献   

20.
An introgression line derived from an interspecific cross between Oryzasativa and Oryza officinalis, IR54741-3-21-22 was found to beresistant to an Indian biotype of brown planthopper (BPH). Genetic analysisof 95 F3 progeny rows of a cross between the resistant lineIR54741-3-21-22 and a BPH susceptible line revealed that resistance wascontrolled by a single dominant gene. A comprehensive RAPD analysisusing 275 decamer primers revealed a low level of (7.1%) polymorphismbetween the parents.RAPD polymorphisms were either co-dominant (6.9%), dominant forresistant parental fragments (9.1%) or dominant for susceptible parentalfragments (11.6%). Of the 19 co-dominant markers, one primer,OPA16, amplified a resistant parental band in the resistant bulk and asusceptible parental band in the susceptible bulk by bulked segregantanalysis. RAPD analysis of individual F2 plants with the primerOPA16 showed marker-phenotype co-segregation for all, with only onerecombinant being identified. The linkage between the RAPD markerOPA16938 and the BPH resistance gene was 0.52 cM in couplingphase. The 938 bp RAPD amplicon was cloned and used as a probe on122 Cla I digested doubled haploid (DH) plants from aIR64xAzucena mapping population for RFLP inheritance analysis and wasmapped onto rice chromosome 11. The OPA16938 RAPD markercould be used in a cost effective way for marker-assisted selection of BPHresistant rice genotypes in rice breeding programs.  相似文献   

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