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东方百合鳞片快繁及采后处理 总被引：5，自引：0，他引：5
东方百合是世界高档的切花之一,我国百合种球生产均处在研究探索阶段,至今尚无一家大型的种球生产基地或企事业进行百合商品种球的规模化生产,品种选育和商品球供应已成为公认的制约我国球根花卉产业发展的“瓶颈”。笔者公司对此进行了立项研究,把荷兰的技术在中国的气候和土壤条件下应用和再研究,形成一整套在中国进行东方百合种球的鳞片快速繁育综合技术,以弥补中国东方百合种球繁育技术研究的不足,降低繁育成本,提高经济效益。文章介绍了此项研究及其结果。 相似文献
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百合种球反季节培育关键技术研究 总被引：1，自引：0，他引：1
对云南省海拔1100m～2450m地区于春、夏、冬三季播种的百合灰霉病发病指数、种球成球率及品质进行了比较,结果表明:露地春植百合品种Siberia、Toro,灰霉病发病指数明显高于大棚种植,最高达36.2%,种球成球率明显低于大棚种植,最低仅33%.露地夏植百合灰霉病发病指数普遍较低,最低仅1.0%,种球成球率最高达到75%.露地冬植百合未发生包括灰霉病在内的任何叶部病害,各试验点灰霉病发病指数为0,生长季月均温18.5℃的试验场成球率为78%,生长季月均温超过20℃的试验场成球率为17%、25%.夏植、冬植还可提高百合种球外观质量、保持种球内在品质. 相似文献
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近几年来,为降低生产成本,摆脱生产者长期以来对进口种球的依赖,以云南等省市为主的众多科研单位、企业不断推进百合种球的国产化研究和生产进程。目前,我国在百合籽球组培、商品球扩繁、种球分级处理及百合品种选育等方面已取得了一定成果,百合种球的国产化率明显提高。 相似文献
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东方百合快繁培养基优化与脱毒技术研究 总被引：1，自引：0，他引：1
摘 要：【研究目的】为实现百合种球的国产化,繁育大量的优质种球,笔者以东方百合的西伯利亚和索邦品种为材料,研究东方百合茎尖诱导、继代增殖和生根的最佳培养基,并比较不同的组织培养方法的脱毒效果。【方法】设置不同的激素浓度梯度筛选东方百合茎尖诱导、继代增殖和生根的最佳培养基,并比较新鲜百合球茎尖培养、冷藏处理百合球的茎尖培养、鳞片培养、鳞片扦插苗茎尖培养和试管苗茎尖培养等5种组织培养方法的脱毒效果。【结果】试验结果表明,百合茎尖诱导的适宜培养基为MS + 2.0mg/L 6-BA + 0.1mg/L NAA,芽形成的适宜培养基为MS + 1.0mg/L 6-BA + 0.5 mg/L NAA,苗形成的适宜培养基为MS + 1.0mg/L 6-BA + 0.08mg/L NAA,生根的适宜培养基为1/2MS + 0.4mg/L NAA + 4%AC (Active Charcoal)。病毒检测表明试管苗茎尖培养的脱毒效果最好,但成活率较低;冷藏处理百合球的茎尖培养的脱毒效果也较好且成活率较高。【结论】筛选的东方百合各阶段的培养基配方能满足东方百合快速扩繁的技术要求,茎尖培养在东方百合依然是较好的脱毒方法。 相似文献
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以不同熟性7个大蒜品种为试材,研究了播前低温处理对大蒜鳞茎POD活性和可溶性蛋白质含量的影响。结果表明,低温处理后7个品种的植株鳞茎POD活性在鳞茎形成期均低于相应对照,可溶性蛋白质含量变化不同。鳞茎POD 活性和可溶性蛋白质含量均呈降低趋势;晚熟品种鳞茎POD活性呈现高于中、早熟品种的趋势。 相似文献
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东方百合不同培养方式鳞茎淀粉粒亚显微结构比较研究 总被引：1，自引：1，他引：0
为了研究东方百合鳞茎发育的机理问题,以东方百合‘索蚌’为试材,进行了不同培养方式下鳞茎淀粉含量、可溶性糖含量、蛋白质含量等生理指标及淀粉粒亚显微结构的比较研究。结果表明：商品鳞茎淀粉粒数量明显比扦插鳞茎、试管鳞茎多,含水量比扦插鳞茎、试管鳞茎少,这与淀粉含量测定结果一致;商品鳞茎蛋白质含量最低、试管鳞茎最高;商品鳞茎基部、中部、上部都有大小淀粉粒并存,扦插鳞茎及试管鳞茎淀粉颗粒大小较一致,差异不明显;无论是商品鳞茎、扦插鳞茎还是试管鳞茎,基部淀粉粒数量都明显比中部和上部多。 相似文献
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Summary An efficient procedure for identification of tulip cultivars in dry bulb stage, based on polymorphism in the isozymes of esterase, is described. Out of 91 cultivars (excl. mutants) which were analyzed for their esterase banding patterns, 78 (86%) could be uniquely identified using native PAGE of bulb protein extracts. A group of 14 Darwin hybrids showed strongly resembling banding patterns; they all originate from the same breeder and most likely have the same parents. Mutants could not be discriminated. The esterase patterns were not influenced by type of bulb scale used for extraction (inner vs outer scale), bulb size (mature bulb or clister) and bulb origin (different lots, grown on sand or clay). However, bulb storage did affect the esterase pattern in several cultivars; generally, some bands appeared or became more intense in the lowest region of the banding pattern after storage of the bulbs. 相似文献
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百合种球活力改良外源技术研究进展 总被引：2，自引：0，他引：2
为了提高百合种球活力研究的系统性,文章从种球活力外源改良技术研究的有关进展入手,包括种球品质复壮技术、采后处理技术和播前处理技术等三大方面,系统阐述组织培养、脱毒、扦插、种球更新、提纯复壮、冷藏处理和播前处理的理化调节技术等应用进展,认为这些外源技术的应用直接受品种、类型、状态、环境、处理等因素影响,改良效果存在差异,建议百合种球外源改良技术的研发应用宜依托种质创新、种球活力生理等基础研究,形成较为完备的种球品质精准改良技术的研发体系,以推动中国百合种球国产化繁育工作健康发展。 相似文献
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Heritability and Correlation Studies on Some Vegetative Traits in Nigerian Local White Onion, Allium cepa L. 总被引：1，自引：0，他引：1
Heritability estimates for soluble solids, bulb firmness and bulb weight were carried out in two ways, namely, parent-offspring regression and half-sib family intra-class correlation. Leaf characters were estimated only by half-sib intra-class correlations. For soluble solids the values were high (72% and 58% respectively), while the values for bulb weight And bulb firmness were low (20% and 39%, and 32%, and 33% respectively) using the two methods. Some high additive genetic correlations were observed between bulb firmness and length of longest leaf (rA= 0.830), bulb weight and leaf area of longest leaf (rA= 0.704). 相似文献
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Summary The suitability of image analysis was studied to measure bulb characteristics for varietal testing of onions (Allium cepa L.). Eighteen genotypes were used, which covered a whole range of onion shapes, including some quite identical ones. The characteristic height and diameter were measured both by image analysis and by hand. The methods gave comparable results with regard to precision and discriminating power. The discriminating power of the image analysis method could be increased by adding a new characteristic: the relative position of the diameter.For determining the height of the bulb with image analysis two methods are described to locate the top of a bulb. One method is to use the location of the inflection point as the top of the bulb. For most bulb shapes, this point coincides with the top of the bulb as indicated by a crop expert. For ovate shaped onions, however, the inflection point is below the top of the bulb as indicated by the crop expert. The other method is to use the location of the intercept of the tangent at the inflection point with the length axis of the bulb as the top of the bulb. This point is always above the top as indicated by the crop expert. Moreover it seems to perform slightly worse for the discrimination of cultivars. Therefore the location of the inflection point as the top of the bulb is preferred. 相似文献
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为研究建立高效稳定的百合鳞茎形成体系,以自繁的东方百合(Lilium Oriental Hybrid)品种‘索蚌’为材料,研究SOD、POD、CAT 3种抗氧化酶的活性变化以及可溶性蛋白和可溶性糖含量变化对百合鳞茎发生过程中的生理生化机制。结果表明,在百合鳞茎发生发育过程中,SOD与POD活性升高,而CAT酶活性的变化与SOD、POD活性变化趋势正好相反;可溶性蛋白累积和可溶性糖变化与百合鳞茎形成密切相关。因此,在百合鳞茎形成过程中,SOD、POD、CAT酶活性变化与鳞茎诱导及其发育密切相关,其中SOD、POD酶在百合鳞茎形成的过程中起着主导作用;可溶性糖和可溶性蛋白质累积变化为百合鳞茎形成提供物质和能量基础。 相似文献