共查询到16条相似文献，搜索用时 171 毫秒
1.
运用牛磺脱氧胆酸钠和CpG DNA配合鸭源禽流感H9N2灭活病毒滴鼻免疫雏鸭,通过检测鸭呼吸道各组织中IgA和IgG抗体分泌细胞面积的变化对免疫效果进行评价.结果发现:应用牛磺脱氧胆酸钠和CpG DNA配合H9N2灭活病毒鼻腔免疫后3、5和7周,鼻腔、气管和气管叉组织中的IgA和IgG分泌细胞面积显著或极显著(P＜0.05或P＜0.01)高于单独H9N2灭活病毒免疫后的水平;应用CpG DNA配合H9N2灭活病毒鼻腔免疫后鼻腔和气管组织中IgA和IgG分泌细胞的面积有部分提高(P＜0.05);而单独运用H9N2灭活病毒鼻腔免疫后IgA和IgG分泌细胞面积同对照组相比没有显著差异.结果表明:在牛磺脱氧胆酸钠和CpG DNA的配合下,禽流感H9N2灭活病毒鼻腔免疫能够提高雏鸭呼吸道组织中IgA分泌细胞和IgG分泌细胞的面积,有效地增强呼吸道局部的免疫应答水平. 相似文献
2.
几种黏膜免疫佐剂对鸡小肠IgA分泌细胞的影响 总被引：8，自引：2，他引：8
分别在新城疫Ⅳ系弱毒苗中添加黏膜免疫佐剂乳酸杆菌、CpG DNA、重组IL-2、氟化钠和大豆黄酮，经口免疫鸡后，研究十二指肠、空肠、Peyer’s斑单位面积IgA分泌细胞的变化。首先提纯鸡IgA和制备兔抗鸡IgA血清，然后应用免疫组化技术显示鸡小肠IgA分泌细胞。结果表明，在免疫后第3周、第5周乳酸杆菌组比新城疫组（ND）极显著增加各段小肠IgA分泌细胞的数量（P〈0．01）；CpGDNA、重组IL-2和大豆黄酮在整个免疫期内均明显增加鸡小肠黏膜局部IgA分泌细胞数量；NaF对鸡体黏膜局部IgA分泌细胞数量无明显增加。结果表明乳酸杆菌、CpGDNA、重组IL-2和大豆黄酮是有效的口服黏膜免疫佐剂。 相似文献
3.
《畜牧兽医学报》2016,(6)
拟研究左旋咪唑、聚乙烯亚胺和聚肌胞分别配合灭活H5N1禽流感病毒口服免疫雏鸡,空肠、回肠中IgA和IgG抗体分泌细胞数量和分布的变化。应用左旋咪唑、聚乙烯亚胺和聚肌胞分别配合灭活H5N1禽流感病毒经口免疫雏鸡后,通过免疫组织化学方法显示空肠、回肠IgA和IgG分泌细胞。聚乙烯亚胺配合灭活禽流感病毒口服免疫后,空肠、回肠IgA和IgG分泌细胞的数量均显著(P0.05)高于单独口服灭活禽流感病毒免疫后的数量;左旋咪唑和聚肌胞分别配合灭活禽流感病毒口服免疫后,空肠、回肠IgA分泌细胞数量显著(P0.05)增加,IgG分泌细胞数量无明显变化。研究表明,左旋咪唑、聚肌胞和聚乙烯亚胺分别配合灭活禽流感病毒口服免疫雏鸡,均能提高肠道局部体液免疫水平,聚乙烯亚胺的效果最好,价格更便宜。 相似文献
4.
用添加CpG的禽流感病毒（H5亚型）灭活疫苗对7日龄罗曼公鸡进行鼻腔免疫，14日龄加强免疫一次；分别于首免后第3、5、7周剖杀，取鼻腔、喉部、气管、气管叉及肺组织，通过甲苯胺蓝染色法显示肥大细胞，观察呼吸道各部位肥大细胞的分布及数量变化。结果显示，首免后第3周气管和气管叉黏膜中的肥大细胞数量显著增加（P〈0．05），第5周时喉部和气管叉黏膜中的肥大细胞数量显著增加（P〈0．05），气管黏膜中肥大细胞数量也极显著增加（P〈0．01），第7周肺中的肥大细胞数量显著增加（P〈0．05）；肥大细胞的数量在喉部最多，气管叉次之，气管和肺较少。试验结果表明，局部黏膜免疫可增加呼吸道肥大细胞的数量，肥大细胞可能参与黏膜免疫反应。 相似文献
5.
猪呼吸道IgA和IgG分泌细胞的分布 总被引：1，自引：0，他引：1
旨在研究猪呼吸道IgA和IgG分泌细胞的分布。取健康50日龄猪的软腭扁桃体、咽扁桃体、气管和肺组织,应用免疫组织化学技术研究猪呼吸道中IgA和IgG分泌细胞的变化。结果显示,在软腭扁桃体中,IgA和IgG分泌细胞主要分布在隐窝上皮下;在咽扁桃体中,IgA和IgG分泌细胞主要集中分布在黏膜上皮下;在气管中,IgA和IgG分泌细胞主要分布在黏膜上皮下固有层和气管腺周围,在气管上皮之间也有少量IgA分泌细胞分布;肺中的IgA和IgG分泌细胞主要集中分布在肺泡隔和各级支气管的黏膜上皮下。另外,我们观察到肺中的2种分泌细胞着色明显比气管中淡。对2种分泌细胞进行统计学分析发现,IgA分泌细胞在气管中分布最多,其次是咽扁桃体、软腭扁桃体,肺中最少;而IgG分泌细胞则在咽扁桃体中最多,其次是气管、肺,软腭扁桃体中最少。结果表明,猪呼吸道含有较多的抗体分泌细胞,但不同部位不同的抗体分泌细胞分布不尽相同,该结果可为猪呼吸道黏膜免疫及相关疫病发生机理的研究提供理论依据。 相似文献
6.
7.
口蹄疫病毒(foot-and-mouth disease virus,FMDV)主要通过呼吸道传播,切断呼吸道传播途径能有效的防御口蹄疫。本研究应用O型口蹄疫灭活病毒配合枯草芽胞杆菌通过喷鼻气雾免疫牛来探讨对牛呼吸道黏膜免疫应答及系统免疫应答的影响。结果显示,免疫后牛鼻黏膜与肺内支气管黏膜中的IgA分泌细胞的数量极显著增多(P0.01),而常规口蹄疫灭活苗对IgA分泌细胞数量的影响不大(P0.05);同时,ELISA结果显示鼻、气管、肺和肺内支气管中IL-12、TNF-α水平均极显著增加(P0.01),尤其是在气管和肺内支气管中最为明显,但IL-6无明显差异(P0.05)。从免疫第3天开始牛鼻液、唾液中口蹄疫特异性抗体SIgA以及血清中O型口蹄疫病毒特异性抗体均显著增加(P0.01或P0.05),且维持至3个月。本研究结果表明O型口蹄疫灭活病毒配合枯草芽胞杆菌喷鼻后可刺激牛呼吸道局部体液免疫和细胞免疫,同时还可诱导全身系统免疫应答,本研究为口蹄疫灭活病毒鼻腔免疫提供理论基础和应用前景。 相似文献
8.
断奶仔猪胃肠道中IgA和IgG分泌细胞的分布 总被引：2，自引：2，他引：0
本研究旨在探讨IgA和IgG分泌细胞在断奶仔猪胃肠道不同部位的分布特点和规律。选取8头断奶24 d的仔猪,采集胃底部、胃体部、胃幽门部、十二指肠、空肠、回肠和结肠组织样品,应用免疫组织化学方法检测胃肠道中IgA和IgG分泌细胞的变化。结果显示:IgA和IgG分泌细胞在胃肠道中具有类似的分布特点和规律,即2种分泌细胞主要分布在黏膜固有层中,其数量沿着胃肠道呈现先增加后减少的趋势,其中IgA和IgG分泌细胞在胃幽门部和小肠分布最多。结果表明仔猪的胃幽门部和小肠前段可能是发挥黏膜免疫反应和抵御外界病原微生物入侵的重要位点。 相似文献
9.
分泌IgA和IgG的浆细胞是黏膜免疫系统中两种重要的效应细胞。本研究旨在探究分泌IgA和IgG的浆细胞在家兔鼻腔中的分布特征。以20只健康成年家兔为研究对象，根据硬腭褶皱和牙齿特征，依次将家兔鼻腔分为Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ 5段，运用组织学、免疫组织化学、图像分析及统计学方法，对分泌IgA和IgG的浆细胞在家兔鼻腔中的分布特征进行了详细的研究。免疫组化结果显示：分泌IgA和IgG的浆细胞在家兔鼻腔每段都有分布，主要弥散分布于鼻黏膜固有层中；其形态呈圆形或卵圆形，核一般位于细胞一侧，胞质丰富呈阳性反应。统计结果显示：分泌IgA和IgG的浆细胞在鼻腔不同段的分布密度由高到低依次是：鼻腔第Ⅳ段、第Ⅴ段、第Ⅲ段、第Ⅱ段、第Ⅰ段。其中分泌IgA的浆细胞在鼻腔Ⅲ、Ⅳ、Ⅴ段的分布密度显著高于鼻腔Ⅰ、Ⅱ段（P<0.05），在上颌鼻甲、鼻中隔和筛鼻甲的分布密度显著高于上鼻甲（P<0.05）；与分泌IgA的浆细胞相比，分泌IgG的浆细胞除鼻腔第Ⅳ段的分布密度显著高于鼻腔Ⅲ、Ⅴ段（P<0.05）外，其分布变化趋势基本与分泌IgA的浆细胞类似。结果证明，分泌IgA和IgG的浆细胞弥散分布于家兔每段鼻腔的鼻黏膜固有层中，有利于SIgA和IgG分子在整个鼻黏膜形成完整的保护屏障；分泌IgA和IgG的浆细胞主要分布在家兔鼻腔Ⅲ、Ⅳ、Ⅴ段及上颌鼻甲、筛鼻甲和鼻中隔，提示这些区域是家兔鼻腔免疫的重要效应部位。本研究为家兔呼吸系统疾病的预防及进一步探讨家兔鼻腔免疫应答的机制提供重要的理论依据。 相似文献
10.
分别应用复合黏膜免疫佐剂Ⅰ和Ⅱ与新城疫Ⅳ系弱毒苗混合（佐剂Ⅰ组和佐剂Ⅱ组）,经口免疫鸡后,研究鸡小肠黏膜局部CD3^＋T细胞、上皮内淋巴细胞和干扰素-γ（Interferon-γ,IFN-γ）mRNA表达水平的变化。结果表明,在整个免疫期内CD3^＋T细胞和上皮内淋巴细胞数量都呈上升趋势,在首免后第3和第5周时十二指肠CD3^＋T细胞佐剂Ⅱ组比佐剂Ⅰ组显著增加（P〈0.05）,空肠CD3^＋T细胞佐剂Ⅱ组比佐剂Ⅰ组极显著增加（P〈0.01）,第7周时Peyer’s斑上皮内淋巴细胞佐剂Ⅱ组比佐剂Ⅰ组极显著增加（P〈0.01）;整个免疫期内佐剂Ⅱ组和佐剂Ⅰ组IFN-γmRNA表达水平呈下降趋势,表达量较低。本研究表明2种复合黏膜免疫佐剂都能明显增加黏膜局部免疫细胞的数量,提高小肠局部黏膜免疫力。 相似文献
11.
Hong-tao Chang Xiu-yuan He Yu-Feng Liu Lu Chen Quan-hai Guo Qiu-ying Yu Jun Zhao Xin-wei Wang Xia Yang Chuan-qing Wang 《Journal of veterinary science (Suw?n-si, Korea)》2014,15(3):399-407
A recombinant replication-defective adenovirus expressing the major epitopes of porcine circovirus-2 (PCV-2) capsid protein (rAd/Cap/518) was previously constructed and shown to induce mucosal immunity in mice following intranasal delivery. In the present study, immune responses induced by intranasal immunization with a combination of rAd/Cap/518 and cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODN) were evaluated in mice. The levels of PCV-2-specific IgG in serum and IgA in saliva, lung, and intestinal fluids were significantly higher in the group immunized with rAd/Cap/518 and CpG ODN than animals immunized with rAd/Cap/518 alone. The frequencies of IL-2-secreting CD4+ T cells and IFN-γ-producing CD8+ T cells were significantly higher in the combined immunization group than mice immunized with rAd/Cap/518 alone. The frequencies of CD3+, CD3+CD4+CD8-, and CD3+CD4-CD8+ T cells in the combined immunization group were similar to that treated with CpG ODN alone, but significantly higher than mice that did not receive CpG ODN. PCV-2 load after challenge in the combined immunization group was significantly lower than that in the phosphate-buffered saline placebo group and approximately 7-fold lower in the group treated with CpG ODN alone. These results indicate that rAd/Cap/518 combined with CpG ODN can enhance systemic and local mucosal immunity in mice, and represent a promising synergetic mucosal vaccine against PCV-2. 相似文献
12.
SP DJORDJEVIC GJ EAMENS LF ROMALIS PJ NICHOLLS V TAYLOR J CHIN 《Australian veterinary journal》1997,75(7):504-511
Objective To investigate the protective efficacy of a pool of denatured membrane protein antigens of Mycoplasma hyopneumoniae (J strain) in the molecular size range 70 to 85 kDa (F3 antigen) in combination with adjuvants for pigs challenged with M hyopneumoniae .
Design A vaccine efficacy experiment with assessment of serum and respiratory tract antibody responses.
Procedure F3 antigens were emulsified with five different adjuvants. To groups of three pigs per vaccine, four vaccines were given by intramuscular injection, and two vaccines, including one of those given intramuscularly, were given by intraperitoneal injection.
Results Compared to six unvaccinated pigs, animals vaccinated with F3 antigen displayed significantly reduced pneumonia (54% reduction in mean lung score) following experimental challenge. Analysis of post-vaccination, pre-challenge IgG and IgA ELISA antibody absorbances in serum and respiratory tract washings revealed no correlation with lung score. Six weeks after challenge, pigs previously vaccinated intramuscularly mostly demonstrated greater IgG and IgA responses in respiratory tract washings, and greater IgG serum antibody responses, than those vaccinated by intraperitoneal injection.
Conclusion Pigs vaccinated with M hyopneumoniae antigens in the molecular size range of 70 to 85 kDa showed a significant reduction in lung lesions compared with unvaccinated control animals after experimental challenge. IgG and IgA antibody concentrations in serum and respiratory tract washings after vaccination do not provide a useful prognostic indicator of protection from enzootic pneumonia. 相似文献
Design A vaccine efficacy experiment with assessment of serum and respiratory tract antibody responses.
Procedure F3 antigens were emulsified with five different adjuvants. To groups of three pigs per vaccine, four vaccines were given by intramuscular injection, and two vaccines, including one of those given intramuscularly, were given by intraperitoneal injection.
Results Compared to six unvaccinated pigs, animals vaccinated with F3 antigen displayed significantly reduced pneumonia (54% reduction in mean lung score) following experimental challenge. Analysis of post-vaccination, pre-challenge IgG and IgA ELISA antibody absorbances in serum and respiratory tract washings revealed no correlation with lung score. Six weeks after challenge, pigs previously vaccinated intramuscularly mostly demonstrated greater IgG and IgA responses in respiratory tract washings, and greater IgG serum antibody responses, than those vaccinated by intraperitoneal injection.
Conclusion Pigs vaccinated with M hyopneumoniae antigens in the molecular size range of 70 to 85 kDa showed a significant reduction in lung lesions compared with unvaccinated control animals after experimental challenge. IgG and IgA antibody concentrations in serum and respiratory tract washings after vaccination do not provide a useful prognostic indicator of protection from enzootic pneumonia. 相似文献
13.
本文主要研究寡果糖对人源菌群仔猪肠道中IgA和IgG分泌细胞的影响。通过无菌剖腹产获取15头无特定病原菌(specific pathogen free,SPF)仔猪,随机分为三组。第一组为SPF组,经口灌服磷酸钠缓冲液(内含10%甘油)以示对照;第二组为人源菌群(human flora-associated,HFA)组,经口服途径接种人源菌群;第三组为寡果糖(fructo-oligosaccharides,FOS)组,口服途径接种人源菌群且灌喂寡果糖。仔猪饲养于屏障系统内,无菌条件下人工哺育45天。应用免疫组织化学方法进行研究。结果表明:(1)所有仔猪小肠和结肠的固有层中均分布有IgA和IgG分泌细胞。(2)IgA和IgG分泌细胞在十二指肠中分布最多,随着肠段的向后推移IgA和IgG分泌细胞数量有逐渐下降趋势。(3)HFA组和FOS组IgA分泌细胞数量在回肠显著高于SPF组(P<0.01);十二指肠中HFA组IgG分泌细胞数量显著高于SPF组(P<0.01)。(4)FOS组IgA分泌细胞数量在空肠显著高于HFA组外(P<0.05),其他肠段总体上低于HFA组,但差异不显著。本结果提示给新生仔猪接种人源菌群能促进仔猪肠道中IgA和IgG分泌细胞的发育,而寡果糖使肠道IgA和IgG分泌细胞数量呈现下降的趋势。 相似文献
14.
猪支原体肺炎活疫苗（168株）肺内免疫机制研究 总被引：1，自引：0，他引：1
为研究猪支原体肺炎活疫苗(168株)的免疫机制,通过肺内接种免疫5 ～ 10日龄仔猪,并于免疫后不同时间点检测血清中IgG抗体效价、全血中淋巴细胞转化效率、呼吸道局部的IFN-γ浓度和特异性SIgA滴度,于免疫后28 d剖杀采集呼吸道上皮组织,通过扫描电镜法与原位杂交检测法观察疫苗株在呼吸道的存留以及对纤毛的影响情况.结果发现,免疫后猪血液中淋巴细胞转化增强1.52～2.01倍,支气管表面IFN-γ浓度和特异性SIgA滴度持续增加,但血清抗体一直未检测到.扫描电镜与原位杂交检测结果发现疫苗株能有效地黏附在支气管纤毛上皮细胞上,但对纤毛的影响较小.由此表明,猪支原体肺炎活疫苗(168株)通过肺内免疫可有效激活全身细胞免疫及呼吸道局部的黏膜免疫与细胞免疫反应,而且还可以通过黏附支气管纤毛上皮细胞产生占位效应而对上皮组织不产生损伤. 相似文献
15.
Immunity to bovine reproductive infections. 总被引：3，自引：0，他引：3
L B Corbeil R H BonDurant 《Veterinary Clinics of North America: Food Animal Practice》2001,17(3):567-583
Protective immune responses in the genital tract are robust, as shown by convalescent and vaccine-induced immunity. Systemic immunity is crucial for systemic infections that result in reproductive failure (such as brucellosis, leptospirosis, and the systemic forms of C. fetus and H. somnus infection). Although IgA responses can protect against sexually transmitted or venereal infections, systemically induced IgG antibody responses also protect. IgA responses can be induced by immunization of the genital tract, where inductive sites develop after antigenic stimulation. The common mucosal immune system can also be used to induce a genital IgA response, as shown by intranasal vaccination. Lastly, it is necessary to determine which antigens of each infectious agent are protective and which types of immune responses protect best. 相似文献
16.
Hirokazu Hikono Masaji Mase Aya Matsuu Megumi Nakayama Takehiko Saito 《Veterinary immunology and immunopathology》2013,151(1-2):83-89
Because it is expected to induce cross-reactive serum and mucosal antibody responses, mucosal vaccination against highly pathogenic avian influenza (HPAI) is potentially superior to conventional parenteral vaccination. Here, we tested whether intraocular vaccination with an inactivated AI virus induced protective antibody responses in chickens. Chickens were inoculated intraocularly twice with 104 hemagglutination units of an inactivated H5N1 HPAI virus. Four weeks after the second vaccination, the chickens were challenged with a lethal dose of the homologous H5N1 HPAI virus. Results showed that most of the vaccinated chickens mounted positive antibody responses. The median serum hemagglutination inhibition titer was 1:80. Addition of CpG oligodeoxynucleotide 2006 or cholera toxin to the vaccine did not enhance serum antibody titers. Cross-reactive anti-hemagglutinin IgG, but not IgA, was detected in oropharyngeal secretions. In accordance with these antibody results, most vaccinated chickens survived a lethal challenge with the H5N1 HPAI virus and did not shed the challenge virus in respiratory or digestive tract secretions. Our results show that intraocular vaccination with an inactivated AI virus induces not only systemic but also mucosal antibody responses and confers protection against HPAI in chickens. 相似文献