共查询到20条相似文献，搜索用时 31 毫秒
1.
SummaryDormant axillary buds excised from crowns of pineapple (Ananas comosus L., Merr.) cultured on growth regulator free Nitsch medium sprouted after 8–10 d. Sprouted buds produced multiple shoots (7–10 shoots per bud) upon transfer to solidified Murashige and Skoog medium supplemented with 9.67 μM NAA, 9.84 μM IBA and 9.29 μM KIN. Each isolated shoot upon subculture to liquid medium of the same composition further proliferated to form more multiple shoots (60–65 shoots) and were maintained on a gyratory shaker (90–100 rpm). In vitro grown shoots were rooted on White medium supplemented with 0.54 μM NAA and 1.97 μM IBA. In vitro plantlets were established in cups with soilrite and hardened for four weeks. Phenotypic variants such as albinos, white streaked shoots and shoots with elongated internodes were observed in in vitro cultures. Approximately 520 in vitro produced plantlets were established in the field and these plants exhibit somaclonal variation. Thirty-eight plants were found to be yellowish, spineless with anthocyanin streaks and three were anthocyanin rich, spined plants. 相似文献
2.
SummaryAn efficient, reproducible protocol has been developed for in vitro multiplication of Sida cordifolia L. High-frequency, multiple shoots (90%) were obtained indirectly from nodal explants. Callus was induced when nodal explants were cultured on Murashige and Skoog (MS) medium supplemented with 0.5 mg l–1 Kinetin (Kn). These nodal calli were then cultured in order to differentiate multiple shoots on MS medium supplemented with 0.5 mg l–1 Kn plus 0.5 mg l–1 naphthaleneacetic acid (NAA). Roots were induced from these multiple shoots following culture on MS medium supplemented with 0.8 mg l–1 NAA for 4 weeks. Finally, these in vitro plantlets were hardened, acclimatised, and successfully transferred to the field. 相似文献
3.
Prakash Lakshmanan Majid Danesh Acram Taji 《The Journal of Horticultural Science and Biotechnology》2013,88(2):158-163
SummaryEfficient in vitro procedures for mass propagation of four commercially important Echinacea species have been deveoped. Plants of E. angustifolia, E. pallida, E. paradoxa and E. purpurea were regenerated by three methods, namely axillary bud proliferation, adventitious shoot formation and somatic embyrogenesis. Shoot tips obtained from in vitro germinated seedlings, adventitious shoots or somatic embryo-derived plantlets, when cultured on Murashige and Skoog medium enriched with 1 μM 6-benzylaminopurine, 2 μM kinetin, 0.5 μM indole-3-butyric acid and 4 mg–1 paclobutrazol multiplied three-fold within 3–4 weeks in culture. Incorporation of paclobutrazol in the shoot multiplication medium was necessary to recover healthy and robust shoots suitable for rooting. Direct, high-frequency shoot formation on intact leaves of shoots grown on 6-benzylaminopurine and kinetin-supplemented media, an unusual and novel observation made in this study, occurred in all the species studied. Rooting of in vitro developed shoots was achieved relatively easily with Murashige and Skoog basal medium rather than with auxin-enriched media. Culturing of hypocotyl explants on medium containing 3,6-dichloro-o-anisic acid (commonly known as dicamba), or 2,4-dichlorophenoxyacetic acid, resulted in direct somatic embryogenesis in all the species examined. The presence of cytokinin was required for somatic embryo germination, but further development of germinated somatic embryos into normal plantlets occurred in Murashige and Skoog medium. We conclude that the procedures described here could be used for rapid propagation as well as genetic transformation of commerically cultivated Echinacea species. 相似文献
4.
A protocol for the production of complete plantlets through multiple shoots from the cotyledon-derived calli of ash gourd (Benincasa hispida L.) is described. The embryos were excised from mature seeds and cultured on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurin (BAP, 1–5 μM). After 10 days the well-developed green cotyledons from the growing embryos were isolated and cultured on MS medium fortified with 2,4-D (1–6 μM). The cultured cotyledons gave rise to luxuriantly growing calli after 6 weeks. These calli were subcultured on MS medium supplemented with various concentrations of BAP (1–6 μM) alone or in combination with naphthalene acetic acid (NAA, 0.2 and 0.5 μM) for regeneration. The regenerated shoots were multiplied and rooted on quarter strength MS medium supplemented with indole-3-butyric acid or NAA (1–5 μM). The rooted shoots were transplanted to soil with 90% success. 相似文献
5.
The excised bulb scales of amaryllis (Hippeastrum hybridum), cultured on Murashige and Skoog’s medium supplemented with combinations of NAA and kinetin, developed buds or roots or both together. The patterns of organogenesis were governed by the concentration of NAA in the medium. On the other hand, kinetin was not only less effective but was also toxic at high concentrations. 相似文献
6.
《Scientia Horticulturae》2005,106(3):395-401
An efficient and reproducible method for the large-scale propagation of Aerides crispum L. using protocorm and leaf sections has been developed. Protocorm and leaf sections were cultured on Murashige and Skoog (MS) medium supplemented with cytokinins [N6-benzyl adenine (BA), thidiazuron (TDZ), and kinetin (KN), 0.5, 1.0, 2.0 and 5.0 μM], auxins [α-naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), 0.5, 1.0, 2.0 and 5.0 μM] and coconut liquid endosperm (CW: 5, 10 and 15%). The explants developed protocorm like bodies (PLBs) within 5–8 weeks on the growth medium. BA supplemented medium was found best for the induction of PLBs and an optimum of 49.1 and 22.0 PLBs developed from protocorm and leaf sections on medium supplemented with 1 and 2 μM BA, respectively. Upon subculture on basal MS medium, the PLBs differentiated plantlets within 6–8 weeks. The resulting plantlets were successfully transferred to potting mixture and 85% of plantlets survived after green house transplantation. This simple protocol will be useful for large-scale propagation of A. crispum L. 相似文献
7.
An indirect organogenesis regeneration protocol for Opuntia ficus-indica (L.) Mill var “Blanco sin Espinas” is described. One centimeter square cladode explants sections from previously micropropagated prickly pear plants were cultured in Murashige and Skoog (MS) basal medium supplemented with 20 different combinations of 2,4-dichlorophenoxy acetic acid (2,4-D) and benzyladenine (BA). The best calli induction and regeneration response were observed when 2.26 μM 2,4-D and 2.21 μM BA combination was applied to the nopal explants. Regenerating calli was capable of forming new buds when transferred to MS basal medium supplemented with 0.5 μM BA (proliferation medium). Shoot elongation and rooting were achieved on MS medium without plant growth regulators. Excellent acclimatization to greenhouse conditions was observed for all transferred plantlets. By this procedure no morphological differences were observed between the regenerated and mother plants. This protocol may be also utilized to carry out plant regeneration after genetic transformation, in order to develop transformed plants without the presence of chimeric zones. 相似文献
8.
Callus cultures were established from the basal region of in vitro-obtained shoot buds on MS medium supplemented with CH + CW + NAA. Such callus cultures, when grown on MS medium devoid of any growth regulators, regenerated shoot buds and optimum regeneration was obtained on MS + CW (5% v/v) medium. Addition of BA did not enhance shoot bud regeneration, but two variants (albino types) were observed among the BA-induced regenerants. The callus-regenerated shoot buds produced multiple shoots when transferred to MS + NAA + IBA + K medium. The plantlets were induced to root on a modified White's medium + NAA + IBA and subsequently transferred to soil. 相似文献
9.
Niamh A. O’Dowd D. H. S. Richardson 《The Journal of Horticultural Science and Biotechnology》2013,88(6):1013-1020
The in vitro micropropagation of eleven species of Ephedra was investigated. Shoot nodal explants of E. fragilis were cultured on Murashige and Skoog medium supplemented with 3% sucrose, 0.05 jiM 3-indolebutryic acid and 0.0-0.5 |iM kinetin, zeatin or 6-ben- zylaminopurine. In general, the average number of shoots produced per explant increased and the average shoot length decreased with increasing cytokinin concentration. Substitution of 3-indolebutryic acid with 2,4-dichlorophenoxyacetic acid caused callusing and distorted shoot growth. Shoot cultures of ten other species were grown on 0.05 |iM 3-indolebutyric acid with 0.05 kinetin. Indole-3-acetic acid gave healthy rooting. E. equisitina, E. gerardiana, E. minima and E. saxatilis were successfully micropropagated using a single-stage protocol in which shoots were grown and rooted on Sorbarods using half strength Murashige and Skoog medium supplemented with 1% sucrose and 5.0 (iM indole-3-acetic acid. Healthy plantlets were weaned in John Innes No. 1: Perlite (1:1) following treatment with Captan (1.9 g I'1). 相似文献
10.
M. Barghchi P. G. Alderson 《The Journal of Horticultural Science and Biotechnology》2013,88(3):435-445
Proliferating shoot cultures were established from shoot tips and nodal bud segments excised from seedlings germinated aseptically and cultured on Murashige and Skoog medium supplemented with BAP plus NAA. Shoot tip necrosis occurred in some cultures. Cultured shoots were rooted in vitro using MS medium (half strength macronutrients) containing IBA for root initiation, followed by subculture onto hormone-free medium for root development. Rooted shoots were readily established in peat-based compost. 相似文献
11.
12.
N. Ahmad 《The Journal of Horticultural Science and Biotechnology》2013,88(4):585-589
SummaryAn efficient in vitro regeneration procedure using thidiazuron (TDZ) has been developed to allow high frequency, multiple shoot induction from cotyledonary node explants of cluster bean (Cyamopsis tetragonoloba). Shoot bud induction occurred on Murashige and Skoog (MS) medium after 4 weeks in the presence of TDZ, followed by transfer onto shoot multiplication and elongation media containing MS salts, B5 vitamins, and different combinations of auxins and cytokinins. Multiple shoots were induced at all levels of TDZ in the medium, but the best proliferation capacity occurred at 5 µM TDZ. Combinations of auxins and cytokinins showed a stimulatory effect on shoot multiplication and also on the length of the newly formed shoots. Maximum shoot induction [i.e., the highest number of shoots (16.0 ± 0.94) per explant] was obtained on agar-solidified medium containing 5 µM benzyladenine (BA) with 0.5 µM indole-3-acetic acid (IAA). Rooting of in vitro-regenerated shoots was achieved in ex vitro conditions by a pulse treatment with 300 µM indole-3-butyric acid (IBA) for 15 min. Rooted plantlets were transferred to soil where 70 – 75% attained sexual maturity and produced viable seeds under greenhouse conditions. The present regeneration system is efficient and can be used in various in vitro manipulation studies. 相似文献
13.
Rapid development of axillary buds from shoot-tips and nodes of 18 cultivars of Fuchsia hybrida was obtained on solid Murashige and Skoog medium with BAP (1 mg l?1 and an auxin (0.1 mg l?1). NAA as the auxin appeared to be more active than IAA or IBA. Vegetative shoots were subsequently isolated and developed up to 15 supplementary axillary shoots on the same solid medium. Agitated and non-agitated liquid media of the same composition were less effective. One-cm long shoots could be rooted in 20 days in the presence of IBA before being transferred to soil. 相似文献
14.
True-to-type plantlets of Freesia × hybrida Bailey cultivar ‘Royal’ were generated from callus after 27 months of sub-culture in liquid medium. Callus was initiated from young flower pedicels cultured on semi-solid Linsmaier—Skoog (LS) medium supplemented with 5 mg/l of 2,4-D and 0.5 mg/l kinetin, and transferred to the same medium in liquid form without hormones and thereafter sub-cultured every 7–10 days. Liquid cultures with 2.4–4.3 g of callus per 25 ml medium produced largest increases in callus fresh weight. Callus generated the most shoots when cultured on LS medium supplemented with 5 mg/l of kinetin and incubated in the light, while fewer plantlets were produced when no growth regulator or GA3 or PBA were used. Callus cultures incubated in continuous darkness did not form shoots. 相似文献
15.
Young actively growing tissue explants from Alstroemeria inflorescence stem taken at a distance of 1–2 mm below the apex are capable of regenerating buds and roots from which small plantlets can be established. Root and bud regeneration was directly from the stem tissue and not from the callus. White's medium supported growth and regeneration as well as Murashige & Skoog's medium. A higher ratio of auxin to cytokinin resulted in root regeneration, while the reversed ratio promoted bud differentiation. Plantlets were obtained upon bud subculture on a low sucrose medium supplemented with indoleacetic acid but without kinetin. 相似文献
16.
High frequency and direct (without callus) plant regeneration was achieved from whole leaf explants of thornless blackberry (Rubus hybrid) cv. Black Satin (EC No. 381258; PI No. 553272) in vitro. Leaf blade explants from 1-, 3- and 5-month-old mother cultures were cultured on Murashige and Skoog (MS) medium with thidiazuron (TDZ), N6-benzylaminopurine (BAP), indol-3-butyric acid (IBA) and α-naphthalene acetic acid (NAA), alone or in combination. Three-month explants cultured on 0.02 mg l−1 TDZ produced a high regeneration frequency (91.7%) and the most shoots/leaf explant (17.3). The shoot primordia developed within 3 weeks from the point of detachment of the petiole from the leaf blade. The age of the explant source significantly affected the shoot regeneration potential of the leaf explants. Leaves excised from 3-month-old in vitro-cultured shoots performed better than those from 1- and 5-month-old shoots. Shoots rooted best on half-strength MS basal medium with 0.5 mg l−1 IBA and 90% of the plantlets survived acclimatization. The regenerated plantlets were morphologically similar to the mother plants. 相似文献
17.
Kshanika Hirimburegama Niranjini Gamage 《The Journal of Horticultural Science and Biotechnology》2013,88(3):469-475
SummaryBambusa vulgaris (yellow bamboo), is the most commonly cultivated and used bamboo species in many countries. With the increased demand for bamboo, the importance of bamboo plantations has been realized. This would require large quantities of planting material continuously for which tissue culture techniques offer a solution. The propagation of B. vulgaris through nodal-bud culture was studied. Single nodal segments were tested for bud-break and shoot growth on basic Murashige and Skoog (1962) medium (MS) supplemented with different combinations and concentrations of growth regulators. Results suggest that cytokinin is important for bud-break. Gibberellic acid enhances multiple shoot production in this species. The position of the node on the culm appears to affect bud-break and multiplication, middle nodes are the most suitable. Also, removal of prophylls enhances bud-break. The shoots developed from axillary buds could be rooted on MS basic medium at 50% macro and IBA (0.25 μ). Upon transfer to the field (after four weeks in the rooting medium), the shoots developed into true-to-type plants. 相似文献
18.
多花蔷薇假珠芽诱导、体细胞胚发生及植株高效再生 总被引：8，自引：1，他引：7
以多花蔷薇‘无刺3号’成熟种子为外植体,探讨了假珠芽诱导、体细胞胚发生及植株高效再生的方法。结果表明,种子的子叶在添加2, 4-D 0.5～2 mg·L-1的1/2 MS培养基上暗培养30 d后所产生的愈伤组织,接种到添加TDZ 5～20 mg·L-1的1/2 MS培养基上光培养20 d产生初级假珠芽。假珠芽在添加TDZ 10 mg·L-1和GA3 0.1 mg·L-1的诱导培养基上暗培养20 d后,在含麦芽糖的无激素培养基上光培养产生少量次级假珠芽和胚性愈伤组织。假珠芽保存在诱导培养基上。胚性愈伤组织可发育成大量正常体细胞胚,继而再生正常植物。假珠芽可通过上述方法不断诱导体细胞胚和假珠芽,通过这种方法假珠芽已经保存了2年。 相似文献
19.
《Scientia Horticulturae》2005,106(2):237-246
In vitro direct multiple shoot formation from seedling explants of Indian high pungent varieties of Capsicum annuum cv. Arka Abhir (AA) and Arka Lohit (AL) was successfully obtained. We were able to induce regeneration potency in these varieties by inverting the explant. Aseptically grown seedling explants with decapitated roots, apical meristem and cotyledonary leaves were inoculated in an inverted position in bud induction medium comprising of Murashige and Skoog's basal medium supplemented with 2-(N-morpholine) ethanesulphonic acid (MES) buffer along with 26.63 μM benzyl adenine (BA), 2.28 μM indole-3-acetic acid (IAA) and 10 μM silver nitrate. Profuse shoot bud induction with 20–25 shoot buds per explant was obtained. Supplementation of phloroglucinol in the bud induction medium resulted in 17 and 18% enhancement in bud induction response in Arka Abhir and Arka Lohit variety, respectively on the inverted hypocotyls. Auxin transport inhibitor tri-iodo benzoic acid (TIBA) in the bud induction medium resulted in induction of buds in a shorter period of 40–45 days when compared to bud induction (BI) medium which takes 55–65 days for bud induction. These buds were transferred to MS medium containing 2.8 μM gibberellic acid and 10 μM silver nitrate resulting in elongation of shoot buds. Transfer of shoots to MS basal medium induced rooting to produce plantlets. This protocol can be efficiently used for mass propagation and presumably also for regeneration of genetically transformed C. annuum tissues. 相似文献
20.
Shantha M. S. D. Ramanayake 《The Journal of Horticultural Science and Biotechnology》2013,88(5):594-601
Summary92% of embryos excised from fresh mature unripe fruits of Calamus thwaitesii germinated in a modified Y3 medium with 0.05 mg l±1 of 6-benzylaminopurine (BAP). This was higher than the 72% germination obtained with ripe seeds sown in soil. Stored seed lost viability within two weeks due to dehydration of embryos. Germination commenced with the differentiation of the haustorium and the cotyledonary sheath, observable in embryos germinating in vitro. This was followed by the development of the plumule. The first eophylls were simple and lanceloate. Decapitation of the in-vitro seedlings and transfer to a medium with higher levels of BAP at 5 or 8 mg l±1 resulted in the production of multiple shoots after 4±5 months, initially from buds that developed around the collar region. Repeated subculture resulted in the development of a clustering habit similar to that of field clumps with a rhizome, axillary shoots and dormant buds. Two axillary meristems were induced to develop precociously into inflorescences. Incorporation of activated charcoal and alpha-naphthaleneacetic acid (NAA) with 5 or 8 mg l±1 BAP reduced multiple shoot formation and brought about root development. Single shoots or clusters developed roots in a Y3 medium with reduced macro elements and supplemented with NAA (5.mg.l±1) and activated charcoal. Nursery establishment with 65% survival of plantlets was possible. In-vitro culture of excised embryos could be recommended, as propagules could be made available whenever desired by rooting proliferated shoots. It also allowed the safe transport of germplasm. 相似文献