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甘肃小麦部分种质高分子量麦谷蛋白亚基(HMWGS)遗传变异分析 总被引：1，自引：0，他引：1
应用十二烷基磺酸钠——聚丙烯酰胺凝胶电泳（SDS-PAGE）方法,分析了372份甘肃小麦种质的高分子量麦谷蛋白亚基（high molecular weight glutenin subunit,HMWGS）组成,以了解甘肃省小麦种质的遗传变异。研究表明,供试的甘肃省小麦种质可检测到20种HMW亚基变异类型和36种HMW亚基组合类型。地方品种的HMW亚基组合类型较少,而且以Null、7＋8和2＋12模式为主,育成品种的HMW亚基变异类型比地方品种丰富,以1、7＋8和2＋12三种变异类型的出现频率为最高。供试材料HMW-GS的品质评分在4～12分之间,平均得分为7.0,所筛选出的一批含优质亚基组合的种质可供育种工作进一步利用。 相似文献
3.
小麦F4籽粒中Glu-1基因的遗传多态性 总被引：1，自引：0，他引：1
采用10％SDS—PAGE方法分析JY97—1／blosky和JY97—2／blosky各10个株系100个单株500粒F4籽粒的高分子量麦谷蛋白亚基(HMW—GS)组成，结果表明：1．Glu—D1位点的5 10，5 12的遗传在JY97—1／blosky后代中符合一对相对基因的分离。2．JY97—2／blosky后代HMW—GS组成类型变异极其丰富。在G1u—A1，G1u—B1和G1u—D1三个位点上分别检测到2，9和6种不同的亚基类型，在Glu—A1位点上1亚基和null的频率分别为52．85％和47．15％；Glu—B1位点上的变异最丰富，出现20；7 8 9；6 8等七种新的等位基因类型；Glu—D1位点存在2 5 10；2 12，5 10；5 12等四种新的等位基因类型。最优亚基组合1，7 8，5 10的比例为11．84％。3．两个组合中Glu—B1b或及Glu—B1c的不完全表达率分别为2．07％和1．9％。 相似文献
4.
不同HMW-GS小麦品种在不同地区种植品质分析 总被引：3，自引：1，他引：3
通过分析不同高分子量麦谷蛋白亚基（HMW－GS）类型小麦品种在河南省不同纬度、不同经度地区种植的品质表现,结果表明,高分子量麦谷蛋白亚基评分与小麦加工品质密度相关,根据评分可大致评价不同小麦品种的加工品质。在试验区域内,随纬度降低,不同HMW－GS类型小麦品种的主要品质指标均有所下降,但高分子量麦谷蛋白亚基评分较高的品种受生态环境条件影响较小,在纬度相近、经度不同地区种植的小麦品种品质表现不尽一致。研究还对供试小麦品种的加工利用价值和适宜种植区域进行了评价。 相似文献
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小麦杂交后代的高分子量麦谷蛋白亚基组成分析 总被引：1，自引：0，他引：1
以郑州9023和野二二燕4F杂交F2代株系为试材，用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS—PAGE）法鉴定分析了杂交后代的高分子量（HMW）麦谷蛋白亚基组成。结果表明，在Glu-A1、Glu-B1和Glu-D1三个位点上分别检测到2、3和1种亚基类型，3位点结合共出现3种组合类型。其中，在Glu-A1位点上出现了1和N亚基，1优质亚基出现的频率高达66．7％；在Glu-B1位点上，13＋16亚基出现频率最高（55．6％）；Glu-D1位点上出现的全是被世界公认的优质亚基5＋10。1、13＋16、5＋10亚基纽舍类型出现的频率最高（55．6％），该类型兼有1和5＋10优质亚基。 相似文献
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本文报道了28个普通小麦栽培品种的面团流变学性质与胚乳蛋白类型和数量间的相关性。采用不同方法来分析了这些品种的等位基因组成以及影响面筋结构的各种蛋白质的相对数量。无论是面团强度（W）还是抗拉强度／伸长性（P／L）都与等位基因组成无关。具有相同等位基因组成（即对麦谷蛋白而言）的不同小麦表现出不同的面团流变学性质。麦谷蛋白尤其是高分子量（HMW）麦谷蛋白亚基和x型麦谷蛋白对W和P／L性状是最具影响力的成分。这些蛋白质好象能增强面团强度，并且是面筋网纹的主要组分。 相似文献
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甘肃春小麦资源HMW-GS组成研究 总被引：1，自引：1，他引：0
应用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)方法,分析了176份甘肃省春小麦资源的高分子量麦谷蛋白亚基(HMW-GS)组成.结果表明,甘肃省春小麦资源的亚基组成较丰富,共检测到18种亚基变异类型和28种亚基组合类型.地方品种的亚基组合类型较少而且以Null、7+8、2+12为主,育成品种的亚基变异类型比地方品种丰富,以1、7+8、2+12 3种变异类型的出现频率为最高.供试材料HMW.GS的品质评分在4～10分之间,平均得分7.2,所筛选出的一批含优质亚基组合的材料可供育种工作者利用. 相似文献
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《分子植物育种》2016,(9)
为了了解小麦Glu-A3位点基因的等位变异对SDS沉降值的影响,我们对122个小麦品系的Glu-A3位点等位基因组成(分子标记),HMW亚基组成(聚丙烯酰胺凝胶电泳),以及这些小麦的品质参数SDS沉降值进行了鉴定,测量并进行了数据统计分析。方差分析显示,含有各Glu-A3等位基因的小麦品系SDS沉降值均值之间差异显著,Glu-A3位点基因的等位变异能解释SDS沉降值变异的9.09%,7个等位基因中含等位基因glu A3a,glu A3g和glu A3d的品系其平均沉降值都较高,而含glu A3b,glu A3f,和glu A3e的品系其平均沉降值都较低。不同HMW亚基背景下的分析结果也基本符合这个规律。等位基因glu A3e在地方品系中出现的频率很低,但是其中glu A3e所属品系的SDS沉降值均值显著低于所有材料沉降值均值;而在国外引进品种中出现频率最低的等位基因glu A3d,其所属材料SDS沉降值均值却显著大于所有材料的沉降值均值。这些特异的材料可以作为小麦品质改良的亲本,优质的等位基因可作为品质选育的指标。 相似文献
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本试验对来自拟斯卑尔脱山羊草（Triticum tauschii）的一个异常小的y型高分子量（ItMW）麦谷蛋白亚基基因进行了排序。在Clu—D＇1位点上编码的这个基因被称为12．4，是至今在小麦属物种中描述过的最小的HMW麦谷蛋白亚基基因。为了扩增该基囚的编码区域和中心重复区域，本试验根据已公布的HMW麦谷蛋白基因序列设计了寡核苷酸引物；该基因的编码区域和中心重复区域分别产生了1．4和0．85kb断片。对PCR产物进行了克隆和排序。 相似文献
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不同品质类型春小麦HWM—GS形成时间和积累强度及与品质的关系 总被引：16，自引：3，他引：16
供试材料籽粒形成过程中，麦谷蛋白低分子量亚基部分首先出现，随籽粒发育，麦谷蛋白亚基类型逐渐增多，HMW－GS各个亚基在开花后10天内没有形成，开花15天左右，出现Glu-1位点上编码的高分子量X亚基，25天时，所具有的HMW－GS全部形成，随灌浆成熟，HMW－GS各亚基积累呈递增趋势，积累高峰出现在开花20天至成熟，5＋10亚基与具有该亚基供试品种的优良品质之间存在密切关系，但具有2＋12亚基的东农7742的亚基形成早，2与12亚基的高积累量及1和7＋8亚基的高积累使其同样具有优质特性，Glu-1品质评分与沉淀值之间呈显著正相关，能够反映小麦品质，但并不绝对，小麦高分子量麦谷蛋白亚基总积累与沉淀值呈显著正相关，各高分子量亚基与沉淀值之间的相关未达显著水平，但亚基2与12的积累量与沉淀值的相关性强，尤其是12亚基的积累。 相似文献
11.
Allelic variation in high-molecular-weight glutenin subunit Loci of Glu-1 in Japanese common wheats 总被引：2，自引：0，他引：2
Seed storage proteins of 131 Japanese Norin wheat (Triticum aestivum) varieties were fractionated by sodium dodecyl sulfate
polyacrylamide gel electrophoresis to determine allelic make-up in varieties at each of three loci that control high-molecular-weight
(HMW) glutenin subunits. Three alleles were identified at the Glu-A1 locus, six at the Glu-B1 locus and five at the Glu-D1
locus. Twenty-four different, major glutenin HMW subunits were identified and each contained three to five subunits and seventeen
different glutenin subunit patterns were observed for 19 subunits in the 131 Japanese Norin varieties. Fourteen alleles were
identified by comparison of subunit mobility with that previously found in hexaploid wheat. Japanese Norin varieties showed
a specific pattern of allelic variation in glutenin HMW subunits, different from that of Chinese and other country common
wheats in allelic frequency at Glu-1 loci.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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小麦高分子量麦谷蛋白亚基遗传变异及其与品质和其它农艺性状关系的研究 总被引：92，自引：9，他引：92
本研究应用SDS-PAGE技术分析了国内外757份供试材料的高分子量（HMW）麦谷蛋白亚基组成。共发现65种HMW亚基组成，其中53种为常见型，12种为罕见型。国外品种的亚基组合数多于国内品种，国内育成品种又多于国内地方品种。作者还发现了部分罕见亚基，即由Glu-D1编码的亚基2.2+12，2+10，2，12以及前人未曾发现的3个亚基。研究 相似文献
13.
Hiro Nakamura 《Euphytica》2000,112(2):187-193
Variation in the electrophoretic banding patterns of high-molecular-weight (HMW) glutenin subunits of 274hexaploid wheat (Triticum aestivum) varieties from China was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and 27 different major HMW
glutenin subunits were identified. Each variety contained three to five subunits and 29different glutenin subunit patterns
were observed in274 Chinese hexaploid wheats. Seventeen alleles were identified based on the comparison of subunits mobility
with that previously identified in a set of standard hexaploid wheats. The Chinese hexaploid wheats exhibited allelic variation
in HMW glutenin subunit composition and the variation differed from that of Japanese and hexaploid wheats of other countries.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
14.
小麦地方品种高分子量谷蛋白亚基多样性分析 总被引：3，自引：0，他引：3
采用SDS-PAGE方法,对我国9个麦区的76份代表性地方品种的高分子量谷蛋白亚基(HMW-GS)组成比较分析,并探讨其与环境因素(平均海拔、年平均降雨量和年平均温度)的相关性。结果表明,25.0%的品种具有异质性,分别包含2~4种不同HMW-GS组合;在Glu-1位点共检测到14个等位变异,其中Glu-A1、Glu-B1和Glu-D1等位变异数分别为2、7和5;发现了3个新等位变异,包括Glu-B1位点2个和Glu-D1位点1个。所有等位变异构成16种不同的亚基组合类型,以(N, 7+8, 2+12)为主,频率为69.7%。在Glu-1位点上,不同麦区遗传多样性分布存在一定的不均衡性,年平均降雨量和年平均温度与麦区多样性指数呈负相关。推测环境压力可能是地方品种多样性地区分化的重要因素。 相似文献
15.
A total of 50 bread-wheat varieties bred in Pakistan were characterized for the composition of the high-molecular-weight glutenin subunit (HMW-GS). The glutenin subunits, as revealed by sodium dodecyl-sulfate-polyacrylamide-gel electrophoresis (SDS-PAGE) were numbered according to Payne's numbering system and, varieties were accordingly assigned theoretical quality scores. All varieties were found to possess either subunit 1 or 2* coded by the A genome. Subunits 17+18 and 7 + 9 of the B genome and 5+10 of the D genome were predominantly found in this set of varieties. The frequency of the appearance of Glu-l alleles in the varieties was different from that seen in other countries, especially in terms of the absence of the ‘null’ form of the A genome and the presence of novel subunits at the Glu-Bl and Glu-Dl loci. The compositions of HMW-GS were generally of good quality, with more than 50% of the varieties achieving quality scores of 9–10 with a high (8.6) average score. The results in this study indicate that wheat varieties bred in Pakistan have a narrow genetic base in terms of HMW glutenin subunits. It appears that the breeders have been selecting wheat genotypes of glutenin subunits associated with good quality without knowing the actual composition of these subunits. The database established on the basis of these results is useful for wheat-improvement programmes aimed at varietal identification and breeding for good quality parameters. 相似文献
16.
Genetic diversity of high-molecular-weight glutenin subunit compositions in landraces of hexaploid wheat from Japan 总被引：7，自引：0，他引：7
Hiro Nakamura 《Euphytica》2001,120(2):227-234
The high-molecular-weight (HMW) glutenin subunit composition of seed storage protein of 174 Japanese hexaploid wheat (Triticum aestivum) landraces have been examined by using sodium dodecyl sulfate polyacrylamide gel electrophoresis system. Twenty four different,
major glutenin HMW subunits were identified, and each of the landraces contained three to five subunits and 17 different glutenin
subunit patterns were observed for 13 alleles in the landraces. On the basis of HMW glutenin subunits composition, Japanese
landraces showed a specific allelic variation, close to Japanese commercial wheats in HMW glutenin subunits, different from
those in alien hexaploid wheats. Further, it could be concluded that all common glutenin alleles can be found in the 174 landraces
originated from Japan. The variation detected in the glutenin subunits is useful for variety identification, has a bearing
on our understanding of hexaploid wheat genetic resource evolution in Japan, and raises questions concerning the nature of
this genetic variation.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
17.
The high and low molecular weight glutenin subunit polymorphism of Algerian durum wheat landraces and old cultivars 总被引：2，自引：0，他引：2
The high molecular weight (HMW) and B‐zone low molecular weight (B‐LMW) glutenin subunit composition of 45 Algerian durum wheat (Triticum turgidum L. var. durum) landraces and old cultivars were examined by sodium‐dodecyl‐sulphate polyacrylamide gel electrophoresis (SDS‐PAGE). Nine accessions were heterogeneous and presented two or three genotypes. All together, 33 glutenin patterns were detected, including 12 for HMW and 15 for B‐LMW glutenin subunits. Twenty‐four different alleles were identified for the five glutenin loci studied, Glu‐A1 (3), Glu‐B1 (6), Glu‐A3 (8), Glu‐B3 (5) and Glu‐B2 (2). Five new alleles were found, three at Glu‐A3 and two at Glu‐B3. At the Glu‐1 loci, the Glu‐A1c‐Glu‐B1e allelic composition was predominant (31%). For the B‐LMW glutenins, the most common allelic composition was Glu‐A3a‐Glu‐B3a‐Glu‐B2a (36%). The collection analysed shows a high percentage of glutenin alleles and allele combinations related to high gluten strength, together with some others that have not been tested so far. This information could be useful to select local varieties with improved quality and also as a source of genes to develop new lines when breeding for quality. 相似文献
18.
Summary The endosperm storage proteins of 205 Chinese bread wheat cultivars and advanced lines were fractionated by SDS-PAGE to determine their high molecular weight (HMW) glutenin subunit composition. Seventeen alleles were identified: three at Glu-A1, eight at Glu-B1, and six at Glu-D1. The most common alleles were Null, 1, 7+8, 7+9, and 2+12. The results indicate that wheats from different regions differ in their frequencies of HMW glutenin subunits, however, none of the subunits could be related to specific environments. The glutenin quality scores of Chinese wheats ranged from 3 to 10, with an average of 6.7. Increasing quality scores have implications in improving steam-bread making quality for Chinese consumers. On the basis of HMW glutenin subunit composition, Chinese wheats are close to European wheats, especially Italian wheats because several Italian introductions are widely distributed in the pedigrees of Chinese wheat. 相似文献
19.
Due to their broad population diversity, old wheat varieties or landraces play an important role in increasing the genetic
variability of agronomic traits. On these grounds, an analysis was made of the high molecular weight (HMW) glutenin subunit
composition of the old Hungarian wheat variety Bánkúti 1201. It was found that several genotypes with differing breadmaking
qualities can be distinguished for this character. When using old varieties in breeding, it is possible to broaden the genetic
background of characters responsible for breadmaking quality by separating the populations. A more detailed analysis of the
protein composition of germplasm created in this way will be required to obtain a better understanding of this complex character
for its conscious introduction into breeding programmes.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
20.
Summary One hundred and twenty-eight wheat varieties bred in the Soviet Union were screened for the composition of high-molecular-weight (HMW) subunits of glutenin. In general, variability was low compared to that seen in varieties from other countries. However, varieties from different regions showed distinctive patterns, in some cases clearly due to the use of particular parents in certain breeding programmes, but in others possibly due to the adaptive value of particular alleles to the environmental conditions under which the varieties were bred. For example, among spring varieties, the Glu-D1 allele encoding subunits 2+12 was more common in varieties from areas with limited rainfall than was the allele encoding subunits 5+10.The pattern of HMW glutenin subunits amongst varieties with superior bread-making quality showed few differences from that amongst bread-making varieties of lower quality.Abbreviations BMQ
bread-making quality
- HMW
high-molecular-weight
- LMW
low-molecular-weight
- SDS-PAGE
sodium dodecyl sulphate-polyacrylamide gel electrophoresis 相似文献