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1.
The activity and localization of NAD(P)H-tetrazolium reductase, lactate dehydrogenase, isocitrate dehydrogenase, succinate dehydrogenase, glueose-6-phosphate dehydrogenase, α-glycerophosphate dehydrogenase, acid phosphatase, and alkaline phosphatase in the kidney of 11 female pigs were examined.The pig kidney showed a higher activity of NAD (P) H-tetrazolium reductase in the distal tubules compared with the kidney of rat, mouse, rabbit, dog, cat, and man. The activity of succinate dehydrogenase and alkaline phosphatase was the same in the pig kidney as in the kidney of other examined species. In the pig kidney glucose-6-phosphate dehydrogenase precipitated in situ, while in rat and mouse this enzyme has proved to be highly diffusible. 相似文献
2.
Erythrocytes from bovine fetuses contain about 2.4 times higher D-glucose-6-phosphate dehydrogenase activities than erythrocytes from adult cows and bulls. Studying whether this is due to the existence of a special fetal type of enzyme or an increased amount of enzyme in fetal erythrocytes, the sedimentation coefficients of the enzymes have been estimated by s-zonal ultracentrifugation, and compared to normal and deficient human erythrocyte D-glucose-6-phosphate dehydrogenase, s-zonal ultracentrifugations have been performed with a computer optimized isokinetic sucrose gradient. The mainlines in the program used for calculation of sedimentation coefficients are described.Bovine fetal and adult erythrocyte D-glucose-6-phosphate dehydrogenase was found to have the same sedimentation coefficient of 7.4 S which is different from the sedimentation coefficient of 6.4 S of both human types of the enzyme. The sedimentation coefficients of 6-phospho-D-gluconate dehydrogenase from bovine fetal, bovine adult and human erythrocytes were 6 S for all three types of this enzyme.By cellulose acetate electrophoresis bovine fetal and adult D-glucose-6-phosphate dehydrogenase show the same mobility, again differing from the normal and deficient human type.The results of these experiments show that bovine fetal and adult erythrocytic D-glucose-6-phosphate dehydrogenase with respects to molecular parameters are closely related and perhaps identical enzymes.Keyword: D-glucose-6-phosphate dehydrogenase, glucose-6-phosphate dehydrogenase deficiency, bovine erythrocytes, fetal erythrocytes, human erythrocytes, s-zonal ultracentrifugation 相似文献
3.
N S Agar 《Research in veterinary science》1976,20(2):223-224
The concentration of 2,3-diphosphoglycerate, and the activities of the enzymes hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, glutathione reductase and NADH-dependent methaemoglobin reductase in the erythrocytes of newborn and adult sheep were investigated. All the enzyme activities and the concentration of 2,3-diaphosphoglycerate were found to be significantly greater in the erythrocytes of newborn lambs than in those of adult sheep. 相似文献
4.
R. R. Maronpot 《Canadian journal of veterinary research》1972,36(1):55-60
Erythrocytes of 145 sheep representing six breeds were assayed for glucose-6-phosphate dehydrogenase. All sheep had erythrocyte glucose-6-phosphate dehydrogenase values similar to glucose-6-phosphate dehydrogenase deficient erythrocytes of man. Mean erythrocyte glucose-6-phosphate dehydrogenase levels ranged from 0.65 to 1.54 micromoles of reduced nicotinamide adenine dinucleotide phosphate per gram of hemoglobin per minute. Many of these sheep also had low levels and/or unstable reduced glutathione. Sheep with low levels of erythrocyte glucose-6-phosphate dehydrogenase and reduced glutathione were given large doses of oxidizing drugs or fed fresh fava beans to determine if they would develop intravascular hemolysis. No significant hemolysis was detected as a result of drug administration or fava bean ingestion. 相似文献
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T Arai H Ageto T Shinofuji M Sugawara 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(5):1047-1048
Activities of certain cytoplasmic enzymes were measured in bovine T lymphoma (BTL-PC3 cells). The activities of hexokinase, pyruvate kinase and glucose-6-phosphate dehydrogenase in PC3 cells were elevated as much as 2 or 3-fold of those in bovine thymocyte. The high activities of these enzymes derived from activation of glucose metabolism may reflect the high growth potential of PC3 cells. 相似文献
6.
《Veterinary microbiology》1997,58(1):45-52
Red cells glycolytic enzymes attached and nonattached to K88+ Escherichia coli were assayed. Hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, pyruvate kinase, glyceraldehyde-3-phosphate dehydrogenase and glutathione reductase activities, were measured. E. coli with K88ab fimbriae, E. coli with K88ac fimbriae, and isolated K88ab fimbriae were investigated for their effect on the above enzymes. Different changes were obtained with K88ab + bacteria compared with K88ac + bacteria. Purified fimbriae gave a third set of responses. 相似文献
7.
Von F. Sinowatz R. Skolek-Winnisch W. Lipp B. Meierhofer 《Reproduction in domestic animals》1977,12(4):152-159
Inhalt Das histochemische Verteilungsmuster einiger Oxidoreduktasen und Hydrolasen wurde in den akzessorischen Geschlechtsdrüsen geschlechtsreifer and kastrierter Eber untersucht. Von den nachgewiesenen Oxidoreduktasen zeigten die Succinatdehydrogenase, Isozitronensäuredehydrogenase, Glucose-6-phosphatdehydrogenase und Cytochromoxidase nach Kastration einen deutlichen Aktivitätsabfall. Hingegen war die Reaktionsstlirke beim Nachweis der Lactatdehydrogenase und β-Hydroxybuttersäuredehydrogenase in den akzessorischen Geschlechtsdrüsen von Kastraten and nicht kastrierten Ebern gleich. Mit Ausnahme der alkalischen Phosphatase, die in keinem der untersuchten Organe nachgewiesen werden konnte, war beim Nachweis aller untersuchten Hydrolasen (saure Phosphatase, Adenosintriphosphatase, Leucinaminopeptidase, unspezifische Esterase) in den akzessorischen Geschlechtsdrüsen des Ebers ein deutlicher Reaktionsausfall zu beobachten. In den untersuchten Organen kastrierter Tiere waren die Hydrolasenaktivitäten merklich geringer. Das Enzymprofil der akzessorischen Geschlechtsdrüsen des Ebers wurde mit dem anderer Haussäugetiere verglichen und die Androgenabhängigkeit dieser Enzyme kurz diskutiert. Contents Enzyme histochemistry of the accessory sex glands in mature and castrated boars. Enzyme-histochemical investigations on the male accessory sex glands of castrated and non castrated adult boars: The histochemical localization of some oxidoreductases and hydrolases was investigated in the accessory sex glands of castrated and non castrated adult boars. After castration succinate dehydrogenase, isocitrate dehydrogenase, glucose-6-phosphate dehydrogenase and cytochrome oxidase showed a marked loss of their activity, whereas no change in the activity of lactate dehydrogenase und β-hydroxybutyrate dehydrogenase could be observed. All hydrolases, except alkaline phosphatase, displayed a distinct activity in the male accessory sex glands of the boar. In castrated boars the activity of these hydrolases was markedly reduced. The function of these enzymes and the androgen dependency was briefly discussed. 相似文献
8.
P S Brady L J Brady D E Ullrey D R Romsos 《American journal of veterinary research》1978,39(2):267-270
Eight adult female Beagles were fasted for 21 days to investigate responses of the erythrocyte glutathione peroxidase system enzymes. Blood samples were collected once a week. Erythrocyte reduced glutathione was maintained within 10% of base-line value. Hexose monophosphate pathway dehydrogenases showed a significant inverse relationship to glutathione reductase (GR) activity. Further, active GR, as a percentage of total GR, increased as hexose monophosphate pathway dehydrogenase activity fell. A similar relationship between these enzymes has been reported in human glucose-6-phosphate dehydrogenase deficiency. Also, glutathione peroxidase showed an inverse linear response to erythrocytic reduced glutathione. Though the responses of this system are complex, they appear interrelated. 相似文献
9.
To assess the antioxidant status and oxidative stress in bovine theileriosis due to Theileria annulata blood samples were collected from 35 clinically affected cattle referred to Veterinary Teaching Hospital, School of Veterinary Medicine, Urmia University, Urmia, Iran. Complete blood count, piroplasm parasitemia percentage, erythrocyte glutathione peroxidase, superoxide dismutase, catalase and glucose-6-phosphate dehydrogenase activities, malondialdehyde concentration, osmotic fragility test and median corpuscular fragility were determined and the results were compared with those of 50 healthy controls. Of 35 affected cattle, 12 (34.28%) had severe anemia and 23 had mild to moderate anemia and parasitemia varied from 5 to 40%. The activities of erythrocyte glutathione peroxidase, superoxide dismutase and glucose-6-phosphate dehydrogenase were significantly lower (P<0.0001) and the activity of catalase was significantly higher in the affected cattle than in healthy ones (P<0.001). Malondialdehyde concentration in erythrocytes of affected cattle was significantly more than those of healthy cattle (P<0.001). The affected cattle showed increased fragility of erythrocytes, so that median corpuscular fragility (MCF) in affected group was significantly lower than those of healthy group (P<0.0001). Median corpuscular fragility showed a positive correlation with the severity of parasitemia (r=0.81, P<0.0005) and a negative correlation with the activities of GSH-Px (r=-0.78, P<0.0001), SOD (r=-0.71, P<0.0005), catalase (r=-0.53, P<0.018) and G6PD (r=-0.58, P<0.0005). The results of this study suggest that oxidative damage to RBCs may contribute to the pathogenesis of anemia in bovine theileriosis. 相似文献
10.
In vitro alterations induced by a 10 micrograms/ml and 50 micrograms/ml dose each of thiophenate and fenbendazole on the absorptive surfaces of Haemonchus contortus (Nematoda: Trichostrongylidae) were studied. The most significant changes were induced in the gut epithelium. Alkaline phosphatase and adenosine triphosphatase activities were decreased, succinic dehydrogenase activity was increased, while acid phosphatase and glucose-6-phosphatase were completely lost from the intestinal epithelium after treatment with either of the drugs. A stimulatory effect of these two anthelmintics was observe on lactic dehydrogenase and reduced nicotinamide adenine dinucleotide diaphorase distribution. Thiophenate caused an increase in the activities of glutamate dehydrogenase (GDH), glucose-6-phosphate dehydrogenase (G-6-PD) and nonspecific esterases and a decrease in reduced nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-D) activity. Fenbendazole treatment led to the inhibition of GDH, while G-6-PD, NADPH-D, cytochrome oxidase, monoamine oxidase and nonspecific esterase activity remained unaltered in the epithelium. 相似文献
11.
Soybeans inoculated with Phomopis sp. were incubated for weeks and then extracted with methanol and ether, and the culture filtrates were intubated into crops of chicks. Activities of glucose-6-phosphatase and glucose-6-phosphate dehydrogenase in liver were increased, and glycogen stores and plasma glucose concentrations were reduced. Histologically, severe hepatocytic necrosis was found in chicks treated with the toxic culture filtrate. Significant clinical signs were loss of body weight and reduced feed consumption. The metabolic changes responsible for the findings are discussed. 相似文献
12.
J R Easley 《American journal of veterinary research》1985,46(11):2378-2384
The erythrogram (erythrocyte histogram) and red cell distribution width (RDW) were evaluated in 5 purebred horses and 1 pony of mixed breeding with experimentally induced anemia. Four horses were studied for 6 weeks after 20% of their estimated blood volume was removed on each of 2 consecutive days (40% total blood loss; acute blood-loss group). Two horses were given acetylphenyl hydrazine IV daily, until acute Heinz body hemolytic anemia was induced; the 2 horses were then evaluated for 6 weeks. One horse and the pony had 20% of their estimated blood volume removed via phlebotomy once each week for 8 weeks to induce iron-deficiency anemia (chronic blood-loss group); the horse had been partially depleted of iron before the study began. Weekly blood samples were examined for changes in the erythrogram, RDW, mean cell volume (MCV), and erythrocyte glucose-6-phosphate dehydrogenase activity. Fourteen days after acute blood loss, mild increases were seen in the MCV, which persisted to day 42. The RDW was increased at day 14 and remained increased until day 42; however, the percentage increase was double that of the MCV at days 14, 21, and 28. Erythrograms had mild extensions of the right slope at days 14 to 28. Mean erythrocyte glucose-6-phosphate dehydrogenase activity increased in all 3 groups, but individual concentrations were erratic. In the 2 horses with acute hemolytic anemia, modest increases of similar magnitude were seen in RDW and MCV.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
13.
Filarial parasites, Litomosoides carinii and Setaria cervi, showed great susceptibility to the oxidants generated in vitro by the xanthine/xanthine-oxidase system. In order to counteract such injurious effects, both the filariids possessed an active antioxidant enzymes system. Superoxide dismutase, catalase and glutathione peroxidase were detected in appreciable amounts but glutathione reductase and glucose-6-phosphate dehydrogenase in very low quantities. The former three enzymes were also found to be released by the parasites into the ambient medium. The released enzymes may be responsible for scavenging the host-generated oxidants present in the immediate surroundings of the parasites and thereby enabling them to live comfortably in the host. This Institute-based antifilarial agent namely Compound 82/437 which is 2,2'-dicarbomethoxylamino-5,5'-dibenzimidazolylketone, markedly inhibited catalase and glutathione peroxidase of both L. carinii and S. cervi. The compound, therefore, appears to render the filariids prone to H2O2 toxicity leading to penultimate damage. 相似文献
14.
During an investigation into the role of the adrenal gland in stress susceptibility of pigs, the large lipid masses in the inner zona reticularis were seen to be particularly related to sex differences, although stress-susceptible castrates had significantly more lipid in this zone than had stress-resistant castrates. Significant histochemical differences were also found in the activity of glucose-6-phosphate dehydrogenase and succinic dehydrogenase between the sexes and between castrates of differing susceptibility to stress. The differences in histochemical activity suggest a different origin for some of the adrenocortical cells in the inner zona reticularis. 相似文献
15.
Comparative Study of Erythrocyte Glycolytic Enzymes in Man and in Two Species of Lagomorphae 
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下载免费PDF全文 A. Puget Christine Gouarderes H. Vergnes 《Canadian journal of veterinary research》1974,38(3):314-320
The authors made a comparative study of red cell enzyme glycolysis in man and in two species of Lagomorphae, the pika and the rabbit. The activities of the 12 enzymes of Embden-Meyerhoff pathway and of the two dehydrogenases of pentose shunt (glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase) were determined. Phosphoglycerate kinase and pyruvate kinase showed quite similar activities in pika erythrocytes and in erythrocytes from human umbilical cord. The levels of these enzymes differed significantly in the pika and in the rabbit. No differences were noted between pyruvate kinases from the rabbit and the neonatal man. The other activities gave values either identical to those found in the adult man or intermediate between the adult and the neonatal man. In the rabbit the levels of glycolitic enzymes were generally lower than in the pika except for lactate dehydrogenase and glycose-6-phosphate dehydrogenase. Some characteristics of erythrocyte metabolism specific to the pika may account for the differences observed in this species. The influence of red cell age cannot explain the variations observed for no significant reticulocytosis was observed in the circulating blood. The percentages found in the pika and the rabbit were essentially identical at determination. 相似文献
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Antioxidant profile of cataractous English Cocker Spaniels 总被引：2，自引：1，他引：1
Barros PS Angelotti AC Nobre F Morales A Fantoni DT Barros SB 《Veterinary ophthalmology》1999,2(2):83-86
Cataracts have been attributed to oxidative injury in proteins and lipids. Primary defenses that directly protect the lens against oxidative damage include small molecule antioxidants (vitamin C, vitamin E, glutathione and carotenoids) and antioxidant enzymes (superoxide dismutase, catalase, and the glutathione enzyme systems – glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase). In humans, low plasmatic levels of vitamin C, vitamin E and carotenoids have been associated with a high risk of senile cataracts. Dogs are more prone to develop cataracts. A decrease in antioxidant defenses could be responsible for increased lens oxidation and cataract development. In this study we report the levels of erythrocytic enzymatic antioxidants (superoxide dismutase, catalase, glutathione peroxidase and glucose-6-phosphate dehydrogenase) and plasma vitamin C as well as malondialdehyde, in normal and cataractous English Cocker Spaniel dogs. Plasma vitamin C levels were consistently lower in cataractous dogs (20.17 μ m ± 8.2 μ m ) when compared with normal dogs (24.1 μ m ± 9.4 μ m ). These results indicate a possibly decreased synthesis in vitamin C, leading to lower aqueous humor levels of this vitamin. Considering that vitamin C levels in the aqueous humor may be responsible for lens antioxidant maintenance, and that these levels are obtained from plasma secretion through the ciliary epithelium, decreased plasma levels may indicate a decrease in the antioxidant capacity of the aqueous humor. 相似文献
19.
Raymond W. Sweeney Thomas J. Divers David T. Zamos Pamela A. Spencer 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1989,18(4):103-106
Sixty-three blood samples from 10 diarrheic calves were tested for glucose concentration by two methods. Plasma glucose concentration was measured by the conventional glucose-6-phosphate dehydrogenase method in the clinical laboratory, and the results compared to those obtained using a rapid reagent strip test for blood glucose concentration measurement. The rapid reagent strip test result could not be used to make an accurate prediction of the actual plasma glucose concentration as determined by the conventional method, due to the wide variability in actual plasma glucose concentrations corresponding to each rapid test result. 相似文献
20.
Sweeney RW Divers TJ Zamos DT Spencer PA 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1998,18(4):103-106
Sixty-three blood samples from 10 diarrheic calves were tested for glucose concentration by two methods. Plasma glucose concentration was measured by the conventional glucose-6-phosphate dehydrogenase method in the clinical laboratory, and the results compared to those obtained using a rapid reagent strip test for blood glucose concentration measurement. The rapid reagent strip test result could not be used to make an accurate prediction of the actual plasma glucose concentration as determined by the conventional method, due to the wide variability in actual plasma glucose concentrations corresponding to each rapid test result. 相似文献