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1.
Balsam pear bacterial wilt was observed in Nanning, Guangxi, China. Based on bacteriological identification, pathogenicity tests and 16S rDNA sequencing analysis, the pathogen was identified as Ralstonia solanacearum (Smith) Yabuuchi et al. This is the first report of R. solanacearum causing bacterial wilt on balsam pear in China. 相似文献
2.
五味子叶枯病病原菌鉴定 总被引：6，自引：0，他引：6
The pathogen causing leaf blight on Schisandra chinensis (Turcz.) Baill. was isolated from diseased leaf. Based on morphological characteristics and pathogenicity test, the pathogen was identified as Alternaria tenuissima (Fr.) Wiltshire. This is the first report of this fungus infecting S. chinensis. 相似文献
3.
The pathogen causing stem blight was isolated from diseased stem of Schizonepeta tenuifolia Briq.. Based on rDNA-ITS analysis, morphological characteristic and pathogenicity test, the pathogen was identified as Phytophthora nicotianae. 相似文献
4.
After being onto 22nd recoverable satellite of China for 18-day spaceflight under microgravity condition,me growth and pathogenicity of PeniciUium expansum were investigated.Spore germination rate of the spaceflight pathogen was insignificantly lower than that of the ground control.After germination,germ tube elongation of spaceflight pathogen was slower,as well as mycelia growth.However,there was no significant difference according to independent-samples T-test.The consistent results were obtained in vivo.The space. flight pathogen exhibited a little weaker pathogenicity in peach fruit.These findings suggested that the microgravity reduced the growth and pathogenicity of P.expansum.but the effect Was not marked. 相似文献
5.
苍术黑斑病病原鉴定 总被引：2，自引：0，他引：2
The pathogen causing dark leaf spot on Atractylodes lancea was isolated from diseased leaf. Based on morphological characteristics, pathogenicity test, the sequence of ribosomal DNA-ITS, the pathogen was identified as Alternaria tenuissima (Kunze) Wiltshire. This is the first report that this fungus infects A. lancea. 相似文献
6.
蝴蝶兰软腐病中一种新致病菌的分离与鉴定 总被引：2，自引：0，他引：2
Soft rot disease often affects Phalaenopsis amabilis during the growing season. However, the pathogen of the disease is remaining poorly studied. In this study, bacterial strain R1 was isolated from soft rot tissues in Wuhan. The pathogenic, morphological, physiological, biochemical tests and 16S rDNA sequence analysis were carried out. The homology of 16S rDNA sequence between strain R1 and Pseudomonas grimontii was 99.72%, and its physiological and biochemical properties were also similar to those of Pseudomonas grimontiis. All these evidences indicated that strain R1 could be identified as a novel strain of Pseudomonas grimontii. The pathogenicity of the novel isolate was proved according to the Koch's postulates. This is the first report that Pseudomonas grimontii can cause soft rot disease of Phalaenopsis amabilis. 相似文献
7.
广西黑皮冬瓜疫病的病原菌鉴定及其生物学特性 总被引：9，自引：0，他引：9
Isolate PD1 of Phytophthora was isolated from diseased black pericarp wax gourd in Guangxi in 2005. The morphological and biological characters of the isolate were examined, and its sequence of ribosomal DNA-ITS was analyzed. The range of growth temperature of the isolate was 10-37℃ and the optimal one was 31℃. The pathogen was sensitive to 10 mg/kg of malachite green, and its ability for starch utilization was weak. A wide host range of the pathogen was confirmed by artificial inoculation. The sizes of sporangia were (37.5-71.7) μm×(20.8-47.5) μm (av. 55.0 μm×30.4 μm). Microcycle conidiation of the pathogen was observed. The pathogen was identified as Phytophthora drechsleri Tucker based on its morphological and biological characters as well as ribosomal DNA-ITS sequence. 相似文献
8.
阔叶十大功劳炭疽病初报 总被引：3，自引：0，他引：3
A new leaf spot disease of Mahonia healei was found in Guangxi China, which was one of the limitations for the growth of M. healei. Based on the morphology, pathogenicity and the analysis of sequences of ribosome rDNA-ITS, the pathogen of M. healei anthracnose disease was identified as Colletotrichum gloeosporioides (Penz.)Sacc. 相似文献
9.
基于小麦白粉病菌rDNA ITS序列的PCR分子检测 总被引：6，自引：0，他引：6
Wheat powdery mildew(Blumeria graminis f.sp.tritici) is the one of main wheat diseases in China.Based on the internal transcribed spacer(ITS) sequences of ribosome of B.graminis f.sp.tritici,three molecular primer pairs(F1/R,F2/R and F3/R) were designed to detect the fungal pathogen of wheat powdery mildew.The species specificity of these primers was confirmed.F1/R was demonstrated a higher sensitivity than the other two primer pairs,and could detect as low as 1 pg DNA of B.graminis f.sp.tritici.Furthermore,F1/R primer pair was used to detect the pathogen DNA extracted from wheat leaves showing chlorosis and typical symptoms of powdery mildew caused by artificial inoculation with B.graminis f.sp.tritici.The preliminary results demonstrated the usefulness of this primer pair and its potential applications in efficient detection of wheat powdery mildew pathogen from leaves with latent infections at early growth stages of wheat. 相似文献
10.
Fusarium oxysporum is one of the most important phytopathogens and cause Fusarium wilt disease in cucumber, watermelon and melon, etc.In this study, a pair of species-specific primers Fc-1 and Fc-2 was synthesized based on differences in internal transcribed spacer sequences of Fusarium genus.With the primers, a specific 315 bp PCR product was amplified from five F.oxysporum isolates isolated from cucumber, watermelon and melon, infected cucumber and watermelon tissues, while no product was obtained from other fourteen fungi, healthy cucumber and watermelon tissues.The detection sensitivity is 100 fg for genomic DNA of F.oxysporum and 1 000 spores/g soil for the soil pathogens.In contrast, the nested PCR with two pairs of primers(ITS1/ITS4 and Fc-1/Fc-2) increased the sensitivity by 100-fold.In addition, one-step PCR could also detect F.oxysporum in symptomless cucumber root of 7 dpi(days post inoculation) and in infected cucumber and watermelon tissues at the early stage of disease development.Therefore, the developed PCR-based method enabled rapid, sensitive and reliable detection of F.oxysporum.It also provides the detection method for early monitoring and diagnosis of the pathogen as well as the plant disease management guidance. 相似文献
11.
空心菜青枯病病原菌的鉴定 总被引：3，自引：1，他引：2
空心菜青枯病是近年来在广东空心菜产区新发生的病害,病株率一般为5%~20%,严重达50%以上。症状初始表现为植株上部1~2片叶萎蔫、下垂,病叶失去光泽呈灰绿色;随着病情的发展,病叶不断增多,最终整株萎蔫,甚至倒伏。经细菌学鉴定、室内致病性测定、16S rDNA序列比较及系统进化分析,结果表明引起该病害的病原是茄科雷尔氏菌(Ralstonia solanacearum);致病性和碳水化合物利用试验结果表明,该病原菌属茄科雷尔氏菌1号生理小种,生化变种4。 相似文献
12.
净作11年的橘园首次套种芝麻,发现园内芝麻青枯病发生严重。为探明芝麻青枯病初侵染源,本研究从芝麻青枯病株上分离到菌株JXRs02,通过形态学、分子生物学以及聚类分析,鉴定其为青枯雷尔氏菌演化型I。利用针刺接种法,将JXRs02菌株接种至柑橘园中的4种杂草凹头苋、青葙、空心莲子草以及加拿大蓬,发现JXRs02能够侵染凹头苋杂草,造成枯萎症状;LAMP检测的结果显示,在凹头苋病株组织内能够检测到青枯雷尔氏菌。依据柯赫氏法则,利用分子生物学方法证明了对凹头苋杂草具有致病性的菌株和造成芝麻青枯症状的病原菌同为青枯雷尔氏菌演化型I。同时发现,在凹头苋、青葙、空心莲子草和加拿大蓬4种杂草的根际土壤中芝麻青枯病菌均能够定殖繁衍。推测凹头苋杂草可能为芝麻青枯病菌的中间寄主,凹头苋、青葙、空心莲子草和加拿大蓬等杂草根际土壤积累的菌源可能为橘园套种芝麻后青枯病的初侵染来源。本研究对制定作物青枯病的防治策略具有一定科学意义。 相似文献
13.
利用拮抗的Pseudomonas spp.和无致病力的P.solanacearum防治青枯病的研究 总被引：21，自引：1，他引：20
从番茄、烟和木麻黄根围土壤中分离了606个Pseudomonas spp.菌株,94a和22a对番茄、烟和花生青枯病有一定效果。用番茄青枯菌和花生青枯菌通过Co60辐射和紫外光诱变的无致病力菌株,25c、55b对番茄青枯病;45b对花生青枯病;107b对花生青枯病;有一些效果。但不够理想。试验结果证明从植物根围土壤筛选有拮抗作用的P.spp.有可能用于防治青枯病。 相似文献
14.
hrcN是植物病原菌Ⅲ型分泌系统基因（T3SS）的结构基因之一,对病原菌的致病力有重要影响。本研究克隆青枯雷尔氏菌CBM613的hrcN基因并作生物信息学分析,结果显示该基因长度为1320 bp,共编码439个氨基酸,预测HrcN蛋白无信号肽,无跨膜区域,整体呈弱亲水性,该蛋白为ATP酶,定位于细胞内膜和细胞质。基因敲除结果显示,菌株CBM613的hrcN突变体与野生型菌株在富营养培养基上的生长速率差异不明显,而在贫营养培养基上前者的生长速率显著快于后者。敲除hrcN基因后,青枯雷尔氏菌的运动能力无明显变化,生物被膜形成能力显著降低。以敲除突变型和野生型青枯雷尔氏菌株接种番茄,与后者相比,前者的致病力下降,病情指数降低,病程延长,但并未完全丧失致病力,表明hrcN基因是青枯雷尔氏菌致病性相关的重要基因。本研究为进一步探索青枯雷尔氏菌致病机制及防治具有重要意义。 相似文献
15.
青枯病（bacterial wilt）是生姜上的一种细菌土传毁灭性病害,化学防治易污染环境且常规化学药剂很难在土壤中起到持续控病的作用,生物防治是其理想的防治方法。为开发对生姜青枯病有效的生防措施,对从生姜根际土壤中分离的一株生防细菌YB-3进行了鉴定,并对其生物学特性及生防效果进行了研究,以丰富生防菌株资源库。采用抑菌圈法筛选拮抗细菌,对效果最好的拮抗菌株做盆栽试验,测定其对生姜青枯病的防治效果,根据形态特征、生理生化特性以及16S rDNA序列对筛选得到的优良菌株进行鉴定。试验结果表明,该拮抗菌在平皿上对青枯菌的抑菌率为50.67%,盆栽试验对青枯病的防治效果为60.86%;经16S rDNA序列分析,并结合形态学和生理生化特征,将菌株YB-3鉴定为巨大芽胞杆菌Bacillus megaterium。YB-3是一株具有开发利用价值的姜青枯病生防菌株。 相似文献
16.
核盘菌中dsRNA种类及其与致病力的关系 总被引：2，自引：0，他引：2
对源于我国黑龙江省佳木斯市同一块茄子田的14个核盘菌(Sclerotinia sclerotiorum)菌株及其中1个菌株Ep-1PNA5的2个衍生菌株的致病力和菌丝中的双链RNA(dsRNA)进行了分析。在马铃薯琼脂培养基(PDA)上对5个弱致病型核盘菌菌株(Ep-1PD、Ep-1PI、Ep-1PL、Ep-1PM、Ep-1PN)异常性状(菌丝生长慢,且异常分枝)的传染特性进行了测定。结果表明:在离体油菜叶片上,16个供试核盘菌菌株中,7个属于强致病类型,7个属于弱致病类型,2个属于中等致病类型。从10个核盘菌菌株的菌丝中检测到dsRNA因子,并可分成3类dsRNA电泳谱型。第一类dsRNA谱型只含有7.4kb大小的dsRNA分子,3个强致病型核盘菌菌株属于这种谱型;第二类dsRNA谱型含有2种dsRNA分子,大小分别为6.4kb和7.4kb。6个弱致病型菌株属于这种谱型;第三类dsRNA谱型只含有6.4kb大小的dsRNA分子。1个弱致病类型菌株属于这种谱型。从另外6个核盘菌菌株的菌丝中没有检测到任何dsRNA因子,其中4个菌株属于强致病型菌株,2个菌株属于中等致病型。可见,6.4kb大小的dsRNA因子与核盘菌弱致病性状密切相关。5个弱致病型核盘菌菌株(Ep-1PD、Ep-1PI、Ep-1PL、Ep-1PM、Ep-1PN)的异常性状可以通过菌丝接触传染给一些强致病型核盘菌菌株,使其菌丝生长变慢及分枝异常。结果还表明:这些弱致病型核盘菌异常性状的传染具有菌株特异性。 相似文献
17.
姜瘟病病原细菌的鉴定 总被引：8，自引：0，他引：8
我国栽培的姜上发现有几种细菌为害,分别引起叶枯、软腐和蔫萎等症状,而一般所谓"姜瘟"是指为害最严重的萎蔫型细菌病害。根据江苏和山东两地姜瘟病细菌的形态、染色反应、生长适温、培养性状、生理生化反应、血清反应和致病性等性状的测定,并与番茄的青枯病细菌进行比较,证明引起姜瘟的细菌是青枯病假单胞杆菌(Pseudomonas soIanacearum(Smith) Smith)。初步交互接种试验证明,姜瘟菌株与番茄青枯菌株的致病性有一定的差异,姜瘟菌株对番茄的致病性很弱;而番茄青枯菌株则不能为害姜。 相似文献
18.
杭白菊枯萎病是杭白菊重要病害之一，枯萎病的爆发对杭白菊产量造成了巨大的影响。为了明确杭白菊枯萎病的病原菌及其生防菌的防治效果，本文通过形态学和ITS序列分析方法，初步鉴定杭白菊枯萎病病原菌为半裸镰刀菌Fusarium incarnatum。进一步采用菌丝生长速率法和孢子萌发法，测定了淀粉酶产色链霉菌Streptomyces diastatochramogenes 1628代谢产物对该病原菌的抑制效果。结果发现，链霉菌1628代谢产物对菌丝生长和孢子萌发的EC50分别为2.22%和4.70%（体积浓度）。盆栽试验结果表明，施用淀粉酶产色链霉菌代谢产物原液14和28 d后，其对杭白菊枯萎病的保护效果、治疗效果分别为52.84%和42.85%、19.23%和30.16%。说明淀粉酶产色链霉菌1628的代谢产物对杭白菊枯萎病具有明显的保护和治疗作用。 相似文献
19.
不同温度下青枯雷尔氏菌表达谱PPI网络分析发现，yqhE可能是青枯雷尔氏菌新的温度相关致病基因。克隆青枯雷尔氏菌CBM613的yqhE基因，结果发现该基因长度为831 bp，共编码276个氨基酸。基因敲除结果显示，28℃培养的yqhE突变株较野生型菌株致病力降低，病程延长，但并未彻底丧失致病力。20℃和28℃培养的yqhE突变株维生素C的生物合成皆被抑制；与28℃相比，20℃培养下野生型菌株维生素C的合成能力降低。突变株在20℃和28℃培养下甲基乙二醛（MG）和丙二醛（MDA）明显积累，其中20℃积累更多；20℃培养的野生型菌株MG和MDA比28℃积累更多。维生素C的生物合成被抑制导致青枯雷尔氏菌自由基清除能力减弱与氧化应激反应增强，MG和MDA积累产生细胞毒性，上述结果可能与28℃培养的突变株致病力减弱，20℃培养的野生型菌株致病力丧失有关。综上结果表明yqhE是青枯雷尔氏菌温度相关致病基因。 相似文献