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1.
为调查孟津地区引起奶牛流产的主要病原,本实验采集该地区8个规模化奶牛养殖场共52头新鲜流产胎牛,及其母体的血液、阴道分泌物和鼻腔分泌物。对所采集样本进行新孢子虫（N.caninum）、弓形虫(T.gondii)、胎儿三毛滴虫(T. foetus)、布氏杆菌(B.abortus)、牛传染性鼻气管炎病毒（IBRV）和牛病毒性腹泻病毒（BVDV）6种常见流产病原的PCR和RT-PCR检测。结果显示,被检样本总感染率为44.2%（23/52）,其中新孢子虫感染率最高,为32.7%（17/52）;IBRV次之,为13.5%（7/52）;胎儿三毛滴虫、布氏杆菌、弓形虫和BVDV感染率分别为1.9%、1.9%、1.9%和0。11.5%（6/52）的被检样本存在混合感染,其中以新孢子虫+IBRV混合感染为主（50%,3/6）。结合被检流产奶牛的临床特征及饲养管理等因素,推断新孢子虫是导致该地区奶牛流产的主要病原。 相似文献
2.
《黑龙江畜牧兽医》2020,(12)
为了解四川地区奶牛流产病原的感染情况,试验应用血清学方法对8个养殖场中可能造成奶牛流产的布鲁氏杆菌(Brucella)、弓形虫(Toxoplasmagondii)、犬新孢子虫(Neospora caninum)、衣原体(Chlamydia)4种病原进行了调查。结果表明:1 002份奶牛血清样品中布鲁氏杆菌、弓形虫、犬新孢子虫、衣原体的阳性率依次为15.37%(154/1 002)、4.59%(46/1 002)、7.78%(78/1 002)和1.10%(11/1 002),4种病原的总阳性率为28.84%(289/1 002);8个养殖场中,4,8号养殖场的4种致奶牛流产病原的总阳性率最高,分别达到55.00%(33/60)和58.57%(41/70),3,7号养殖场中4种病原的总阳性率相对较低,分别为3.57%(3/84)和2.00%(4/200)。说明四川部分地区奶牛场普遍存在布鲁氏杆菌、弓形虫、犬新孢子虫和衣原体的感染,其中布鲁氏杆菌的感染情况尤其不容乐观,需要进一步加强布鲁氏杆菌病的防控与净化。 相似文献
3.
为了明确河北省内致犊牛感染性腹泻主要病原,本研究自河北省8个地区采集775份犊牛腹泻粪便样品,根据GenBank中登录的牛病毒性腹泻病毒(BVDV)5′UTR基因、牛冠状病毒(BCoV)N基因、牛轮状病毒(BRV)VP6基因和牛传染性鼻气管炎病毒(IBRV)gB基因序列设计特异性引物,参照文献合成隐孢子虫18S rRNA和大肠杆菌16S rRNA引物,利用PCR方法对BVDV、BCoV、BRV、IBRV、隐孢子虫和大肠杆菌进行检测。结果显示,775份犊牛腹泻临床样品中,BVDV的阳性率为27.11%(205/775);BCoV的阳性率为17.52%(137/775);BRV的阳性率为9.85%(77/775);IBRV的阳性率为3.71%(29/775);隐孢子虫的阳性率为12.28%(96/775);大肠杆菌的阳性率为20.46%(160/775);还有9.10%的样品未检测出病原。在河北省部分地区采集的犊牛腹泻粪便样品中,BVDV检测阳性率最高为27.11%,在冬季犊牛BVDV的感染率最高为40.21%,且唐山和张家口地区奶牛场BVDV的感染率高于其他6个地区。本研究明确了河北省内... 相似文献
4.
为了解宁夏主要奶牛养殖区规模化奶牛场牛传染性鼻气管炎(IBR)、牛病毒性腹泻(BVD)、新孢子虫抗体和布鲁菌病原的流行状况,采用酶联免疫吸附试验(ELISA)对未接种过IBR、BVD、新孢子虫疫苗、接种过布鲁菌疫苗的奶牛群体进行血清抗体检测,并对银川、吴忠和青铜峡地区的阳性状况进行分析。结果发现,76份流产牛血清中,IBR抗体阳性率为50.00%(38/76),BVD抗体阳性率为19.74%(15/76),新孢子虫抗体阳性率为27.63%(21/76),布鲁菌阳性率为19.74%(15/76),4种病原均感染的阳性率为34.21%(26/76),IBR和新孢子虫混合阳性率占总阳性率的34.62%;408份非流产牛血清中,BVD抗体阳性率为12.75%(52/408),IBR抗体阳性率为21.08%(86/408);IBR和BVD在宁夏的3个主要养殖区均有不同程度的流行,其中以银川地区阳性率最高。说明宁夏部分地区IBR和BVD在不同的地区存在着不同程度的感染,流产牛中普遍存在上述4种疫病的感染并且IBR和BVD阳性率明显高于非流产牛。 相似文献
5.
《畜牧与兽医》2019,(12):101-105
为了解吉林省牛病毒性腹泻(BVD)、牛传染性鼻气管炎(IBR)、牛呼吸道合胞体病(BRS)的流行及其病原混合感染情况,在吉林省的9个地区随机采集了325份血清样品,采用ELISA血清抗体检测试剂盒与新型纳米PCR方法检测所采集样品。结果显示,ELISA检测BVDV抗体阳性243份,阳性率为74.77%,IBRV抗体阳性186份,阳性率为57.23%,BRSV抗体阳性90份,阳性率为27.69%,BVDV与IBRV混合感染率为31.69%;BVDV与BRSV混合感染率为9.54%,IBRV与BRSV混合感染率为1.54%,3种病毒混合感染率为17.23%。采用纳米PCR方法检测所有血清显示,BVDV抗原阳性42份,阳性率12.92%,未检出IBRV抗原阳性;BRSV抗原阳性29份,阳性率8.92%。BVDV与BRSV混合感染率为1.85%。 相似文献
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7.
[目的]旨在了解河北省廊坊市部分奶牛场流产奶牛布鲁氏杆菌病、弓形虫病、衣原体和犬新孢子虫病感染现状。[方法]选取10家奶牛场有流产病史的荷斯坦牛采集血样265份,应用血清学检测方法对4种病原体进行检测。[结果]布鲁氏杆菌、犬新孢子虫、弓形虫、衣原体的平均阳性率分别为1.13%、3.40%、5.28%和4.53%,最高分别为6.67%、8.70%、9.68%和13.33%。奶牛场阳性率分别为30%、60%、80%和70%。4种病原体总阳性率为14.34%。[结论]廊坊市奶牛场流产奶牛群体种普通存在4种流行病,虽然感染率偏低,但应引起重视,进一步强化防控和净化。 相似文献
8.
[目的]为了解凯里市A奶牛场牛呼吸道疫病的流行情况。[方法]本次采用酶联免疫吸附试验（ELISA）对142 份奶牛血清样品进行牛呼吸道合胞体病毒（BRSV）、牛病毒性腹泻（BVDV）、牛传染性鼻气管炎（IBRV）、牛支原体感染抗体检测。[结果]凯里市A奶牛场4 种呼吸道疾病病原普遍存在,BRSV阳性率为41.5%（59/142）;BVDV阳性率为40.1%（57/142）;IBRV阳性率为45.0%（64/142）;支原体阳性率为33.8%（48/142）。犊牛4 种呼吸疾病单一感染率为15.1%~45.5%;BVDV感染最严重,为45.5%;成年牛单一感染率为33.8%~45.1%。此外,奶牛场存在IBRV、BRSV、BVDV、支原体多种病原体混合感染,二重感染率为18.3％~30.2％;BVDV+IBRV感染率最高,为30.2%;三重感染以BVDV+支原体+IBRV感染率最高,为19.7%;四重感染率为9.9%。[结论]凯里市A奶牛场存在4 种疫病,有不同程度的病原体感染,应加强对上述病原体的流行控制,提高奶牛场管理水平,减少奶牛场疫病发生和经济损失。 相似文献
9.
近年来,我国部分地区奶牛场发生了以流黏性鼻液为主要症状的呼吸道疾病,经检测,排除牛支原体、牛巴氏杆菌感染。为探究其主要病毒性病原,从山东等4省(市)5家表现临床症状的奶牛场采集发病牛与无症状牛鼻拭子样品298份,开展牛传染性鼻气管炎病毒(IBRV)、牛病毒性腹泻病毒(BVDV)和牛副流感病毒3型(BPIV3)PCR检测。结果显示:发病奶牛场IBRV与BVDV阳性率分别为28.9%、7.1%,BPIV3未检出;发病牛与无症状牛IBRV阳性率分别为42.6%、10.9%,BVDV阳性率分别为11.3%、1.6%;IBRV、BVDV混合感染率为5.7%,IBRV、BVDV混合感染占IBRV总感染数的19.8%,占BVDV总感染数的81.0%。结果表明,引起这些奶牛场呼吸道症状的主要病原可能为IBRV、BVDV,且混合感染较严重。本研究为奶牛场呼吸道疾病防控提供了依据。 相似文献
10.
为了调查宁夏吴忠地区奶牛场因传染性因素造成流产的基本情况,应用酶联免疫吸附试验(ELISA)对可能造成感染流产的牛传染性鼻气管炎病毒、新孢子虫、牛病毒性腹泻病毒的抗体及布鲁菌的抗原进行了血清学检测。结果显示,牛传染性鼻气管炎病毒抗体阳性率为48.84%,新孢子虫抗体阳性率为30.23%,牛病毒性腹泻病毒抗体阳性率为19.77%,布鲁菌抗原阳性率为24.42%。牛场阳性率高达100%。根据统计显示,不同妊娠胎次的奶牛流产率差异显著(P0.05),其中第1胎流产率与其他胎次间差异极显著(P0.01)。不同妊娠时间的奶牛流产率差异不显著(P0.05)。调查表明,宁夏部分奶牛场存在不同程度的牛传染性鼻气管炎病毒、新孢子虫、牛病毒性腹泻病毒和布鲁菌感染。 相似文献
11.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1985,26(10):323-327
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.
Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.
相似文献12.
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(9):347-350
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.
Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.
相似文献14.
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Slocombe JO Villeneuve A 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(10):630-633
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
16.
Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(9):324-328
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).
Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.
相似文献17.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1989,30(6):504-508
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
18.
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.
Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.
相似文献19.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1988,29(8):641-646
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
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Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1987,28(8):491-495
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).
Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.
相似文献