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1.
Construction and Characterization of Grass Carp （Ctenopharyngodon idellus） Fosmid Library 总被引：1，自引：0，他引：1
Grass carp （Ctenopharyngodon idellus） genomic fosmid library cotaining 129 014 clones was constructed and characterized from one diploid grass carp. The average insert size of the fosmid library was determined to be 35 kb by pulsed field gel electrophoresis, which is 4.1-fold coverage of the grass carp genome. To demonstrate the probability of picking the functional genes from the library, eleven functional genes were screened by three-dimensional PCR technique. The number of positive clones of these genes was from 1 to 6. So, this library may screen any useful genes from grass carp. This grass carp genome fosmid library will be integrated in the presently ongoing efforts to determine the sequence of the grass carp genome. 相似文献
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茄类内生菌的分离及拮抗细菌的筛选(英文) 总被引：2，自引：0，他引：2
[Objective] The aim of this study was to isolate the endophyte of three solanaceae fruits and vegetables such as tomato, pepper and eggplant, to screen and identify the bacterial wilt antagonistic bacteria. [Method] According to the lapping liquid culture method, the endophyte of three plants was isolated by the selective medium and purified by the plate streaking method, so the purified endophyte was screened by the hyphal pieces confront culture method. Furthermore, the screened antagonistic and endophyteic bacteria was identified and classified through culture characteristics of isolates and morphological features of thallus, Gram stain as well as physiological and biochemical reactions. [Result] Fifty-three endophytic bacteria, fifty-three endophytic fungi and forty-four endophytic actinomycetes were separated from the endophyte of three plants. The screened fourteen endophytic bacteria with strong antagonistic effect on the bacterial wilt were classified to Bacillus, Escherichia, Klebsiella, Agromonas, Erwinia and Curto Bacterium respectively. Especially, Bacillus was the dominant species, which had the strongest antagonistic effect on the bacterial wilt. [Conclusion] This study provides an effective way for biological control of the bacterial wilt in solanaceae. 相似文献
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[Objective] The dormancy characteristics of Malus ‘Snowdrift’ in long-day treatment were studied, and 6-BA was used to break the dormancy, with the aim to achieve the purpose of flowering in autumn. [Method] The new shoots of ‘Snowdrift’ in long-day treatment were conducted with hydroponics to investigate their dormancy time. And cytokinin 6-benzyladenine(6-BA)was used to treat the dormant shoots, to investigate the budding, flowering and flower bud differentiation. [Result] The shoots in long-day treatment entered endodormancy after August 7~(th) and the shoots in the natural daylight entered endodormancy before July 18~(th). In long-day treatment, 116 buds, 198 flowers were observed after 6-BA spraying. [Conclusion] The optimum concentration of 6-BA was 300 mg/L. And the flower bud differentiation of ‘Snowdrift’ in long-day treatment was faster than those in natural daylight after 6-BA spraying. 相似文献
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板栗新害虫深褐拟叶螽生物学特性及发生条件的研究(英文) 总被引：1，自引：0，他引：1
[Objective] The study aimed to explore the occurrence conditions of Tegra novaehollandiae viridinotata on chestnut.[Method] Life history was observed by the combination of indoor-rearing and field observation,as well as the relationship between occurrence and surroundings in field.[Result] T.novaehollandiae viridinotata has one generation each year in Yunhe County,Zhejiang Province,and overwinters by laying eggs in chestnut trunk with diameter of 5.0 cm.Adults and the nymphs feed on Glochidion puberum leaves,adults damage trunks of young chestnut by oviposition.Nymphs hatch in early April,adults moult in last June and lay eggs in middle July.[Conclusion] T.novaehollandiae viridinotata could be controlled by eliminating shrubs such asGlochidion puberum.,cutting the eggs in dormancy by an knife,spraying pesticides in middle April or June and July,coating stem in winter etc. 相似文献
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TONGFu-dan LIUHong-yun 《中国农业科学(英文版)》2004,3(9):700-706
The insulin-like growth factor Ⅰ (IGF-Ⅰ) gene of triangular bream (Megalobrama terminalis)(GenBank No.AY247412) (Tb)was cloned for the first time from liver by RT-PCR. The nucleotide sequence analysis showed the Tb IGF-Ⅰ cDNA consisted of 486 nucleotides and encoded 117 amino acids including B, C, A, D and E five domains. Analysis of E-domainin dicated that cloned Tb IGF-Ⅰ belonged to IGF-Ⅰ Ea-2 subtype. Identity analysis showed the IGF-Ⅰ nucleotide sequence shared 99.8% homology with bluntnose bream, 88.8% with grass carp, 85.8% with common carp; the pre-IGF-[ amine acid sequence shared 99.4% with bluntnose bream, 88.8% with grass carp, 85.4% homology with common carp. In the Cyprinus Carpio, the higher homology of nucleotide sequence and amino acid sequence in IGF-Ⅰ showed that the closer relationship the fishes have. These results could provide basic data for the research on Tb germplasm and the development and utilization of biological feed additives. 相似文献
6.
稻鸭共育对病虫草害及经济效益的影响（英文） 总被引：2，自引：0，他引：2
[Objective] Study on the effects of rice-duck mutualism on weeds and insects pests and economic benefits of paddy field.[Method] Comparison of the incidence of weeds,sheath blight and insects pest under rice-duck mutualism,conventional cultivation and control treatment,the yield and economic benefits were analyzed under the 3 treatments.[Result] Average occurrence of weeds in rice-duck mutualism group decreased by 2.33 and 52.08 ind/m2 compared with that in conventional cultivation and control treatment;the control rate of mutualism was up to 75% against rice hopper,but just between 25%-60% against rice leaf roller and Chilo suppressalis.The rates of diseased plant and diseased holl against rice sheath blight were higher and disease indices were lower compared with control group.The yield of mutualism group was identical with conventional cultivation,which was greatly higher than that of control group.The results suggested an higher economic benefits and lower cost benefit ratio for rice-duck mutualism treatment.[Conclusion] Rice-duck mutualism gives birth to a positive effect to control the diseases,insect pests and weeds,as well as to economic benefits,providing basis on extension of rice-duck mutualism system. 相似文献
7.
生长素介导下三唑醇在大豆植株内的传导性(英文) 总被引：4，自引：0，他引：4
[Objective] This study was to investigate the mobility of IAA-triadimenol and NAA-triadimenol in soybean seedlings. [Method] Soybean seedlings were treated by spraying the solution of conjugated compounds onto their leaves; and chromatography was employed to measure the contents of IAA-triadimenol and NAA-triadimenol in different parts of these soybean seedlings, through which to represent the mobility of the conjugates. [Result] Both the triadimenol and NAA-triadimenol could not transport basipetally; whereas IAA-triademenol was ambimobile. When sprayed with 0.5 mmol/L IAA-triadimenol, as much as 1.87 μg/(g·FW) IAA-triadimenol was detected in the roots of soybean seedlings 12 h later, which was higher than that in the stem [0.68 μg/(g·FW)]; while that decreased to 0.80 μg/(g·FW) in the seedlings sprayed with 0.5 mmol/L IAA-triadimenol and the same concentration of IAA. [Conclusion] IAA moiety of the conjugate could enhance the transport and accumulation of the fungicide towards the root via the IAA carriers. 相似文献
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渗透调节物质及有机附加物对小麦体细胞胚发生影响的研究(英文) 总被引：5，自引：1，他引：4
[Objective] The study aimed to reveal the effect of osmosis-regulating substances and organic appendices on somatic embryogenesis in wheat. [Method] The suitable concentration combination of appendices was optimized by adding different concentrations of osmosis-regulating substances including mannitol, sorbitol and organic appendices such as Gln, CH and LH, into the somatic embryogenesis in wheat. [Result] The mannitol or sorbitol lower than 40 g/L was helpful for improving somatic embryogenesis; there was no significant difference in the induction rate of somatic embryogenesis when 300-500 mg/L Gln、CH or LH was respectively added into the induced medium, while somatic embryogenesis could be enhanced dramatically in the presence of 500 mg/L Gln together with 300 mg/L CH. [Conclusion] Somatic embryogenesis could be improved to some extent by different concentrations of osmosis-regulating substances and organic appendices, which laid foundation for establishing a more perfect system of somatic embryogenesis in wheat. 相似文献
9.
《（《农业科学与技术》）编辑部》2008,(2)
[Objective] The study aimed to reveal the genetic model of a biomass mutant in Oryza sativa. [Method] In the process of screening and identification of Bar-transgenic rice,a biomass mutant was found in 10 lines of T1 progenies. The mutant was investigated for genetic analysis and agronomic traits by herbicide spraying and PCR amplification. [Result] The segregation ratio is consistent with mendelian law(3∶1). The mutant assumed not only higher plant height,wider straw and earlier florescence,but also more tillers,bigger spikes and resultantly higher biomass. PCR detections indicated that no co-segregation was observed between mutant traits and target gene(Bar) in the T-DNA inserted,proving that the mutant is not caused by the insertion of T-DNA containing target gene (Bar). [Conclusion] Our study may avail to understand the cloning of mutant gene and the mechanism of the mutant gene on biomass. 相似文献
10.
[Objective] This study was to improve the biomass of Navicula tenera, and thus to provide reference for achieving the industrial production of Navicula tenera. [Method] The feasibility of using sewage to cultivate Navicula tenera was preliminarily investigated based on the consideration of regional characteristics; and a series of culture conditions including the nutrient source of nitrogen(N), phosphorus(P), iron(Fe), silicon(Si) and the salinity in medium for culturing Navicula tenera, were optimized by single factor test and orthogonal design. [Result] The optimized conditions for cultivating Navicula tenera using sewage are as follows: the water from Xiaoerlou Artificial Lake of Nanjing University of Technology as basic solvent; 360 mg/L urea; 150 mg/L N2HPO4·12H2O; 50 mg/L ferric citrate; 2 000 mg/L Na2SiO3·9H2O; 2.0 mol/L salinity. Navicula tenera was strongly adaptive to sewage and could well uptake the nutrient sources in the sewage. Under the optimized conditions, the culture cost decreased, and meanwhile the biomass of Navicula tenera reached 4.766 g/L which is 3.57 multiples over original medium and 1.9 multiples over optimized medium No. 1. [Conclusion] This study laid a foundation for the combination of culturing Navicula tenera in large scale and sewage treatment. 相似文献
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[目的]对946中草药添加剂防治淡水鱼细菌性烂鳃病效果进行比较试验,为大面积淡水鱼养殖筛选有效药物及防治细菌性烂鳃病提供理论依据。[方法]选择混养淡水鱼塘,分别应用946中草药添加剂、生石灰、畜用红霉素和空白对照组进行比较试验,观察其对淡水鱼细菌性烂鳃病的防治效果。[结果]应用946中草药添加剂和畜用红霉素鱼塘的鱼未发生细菌性烂鳃病;而应用生石灰和未添加药物的空白鱼塘内的鱼均发生严重的烂鳃病。[结论]946中草药添加剂对淡水鱼烂鳃病有明显的防治效果。 相似文献
14.
【目的】观察对比脆肉鲩与普通草鱼肌肉显微结构的差异,为脆肉鲩的肉质加工提供科学依据。【方法】采集脆肉鲩和普通草鱼的背部白肌、背部红肌和腹部白肌,经石蜡组织切片和苏木素-伊红染色后,显微镜下观察肌纤维的形态特征,用肌纤维直径和密度两个指标衡量脆肉鲩与普通草鱼的肌纤维差异。【结果】脆肉鲩和普通草鱼背部白肌、腹部白肌及背部红肌的基本结构相同。脆肉鲩背部白肌的肌纤维直径（67．6μm）显著小于普通草鱼（109．4μm）（P〈0.05,下同）,肌纤维密度（249条／mm2）N显著大于普通草鱼（121条／mm2）;在腹部白肌和背部红肌的肌纤维直径、肌纤维密度方面,脆肉鲩与普通草鱼无显著差异（P〉0．05）。【结论】脆肉鲩肌肉的肌纤维直径及密度与其肌肉硬度具有一定相关性。 相似文献
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HPLC法分析加热对草鱼红肉中滋味物质的影响 总被引：2，自引：0，他引：2
[目的]研究加热前后草鱼红肉中滋味物质的变化。[方法]分别使用高效液相色谱仪和氨基酸自动分析仪分析了草鱼红肉中加热前后核苷酸类物质和游离氨基酸含量的变化。[结果]新鲜草鱼红肉中核苷酸总量为1 088.809μg/g。新鲜草鱼红肉中ATP含量为358.640μg/g,加热后为0。加热后ADP、AMP、IMP、HxR和Hx的含量分别增加15.502、4.232、7.689、44.917和74.688μg/g。新鲜草鱼红肉中游离氨基酸总量为3 129.106μg/g,加热后游离氨基酸总量减少了852.161μg/g。其中,含量增加的游离氨基酸有天冬氨酸、谷氨酸、缬氨酸、甲硫氨酸、亮氨酸、酪氨酸,含量降低的游离氨基酸有苏氨酸、丝氨酸、甘氨酸、丙氨酸、赖氨酸、组氨酸等。[结论]该研究可为今后草鱼热制品的风味研究奠定理论基础。 相似文献
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黄芪多糖防治草鱼出血病的研究 总被引：6，自引：1，他引：6
[目的]防治草鱼出血病,提高草鱼成活率。[方法]用黄芪多糖、维生素K3粉、板蓝根和维生素C等免疫增强剂拌通威饲料在4个水域进行投喂,开展草鱼出血病的初步治疗试验。[结果]水域1草鱼在用药后的第4天基本没有鱼发病死亡,之后没有发生草鱼出血病。水域2草鱼6月24日用药,6月26日病愈,并且没有出现草鱼出血病复发现象。水域3草鱼在用药后第3天完全病愈,之后没有复发。水域4草鱼在用药后第4天死鱼现象停止,之后没有复发。[结论]在草鱼饲料中添加适量的黄芪多糖、维生素K3粉、维生素C,可提高草鱼对病毒的抵抗能力,达到治疗草鱼出血病的效果,添加适量的板蓝根对草鱼有保肝利胆作用。 相似文献
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草鱼四联疫苗在网箱养殖中的试验 总被引：1，自引：0，他引：1
草鱼养殖面积广,是我国传统的优质养殖鱼类,但在养殖过程中病害较为严重。除病毒性出血病外,还流行细菌性烂鳃、赤皮、肠炎、败血症等,苗种期还经常受到车轮虫等寄生虫的侵袭,它们通常交织并发,严重困扰草鱼养殖业的健康发展。 相似文献
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天然复合脱腥保鲜液对草鱼的脱腥保鲜效果 总被引：1，自引：0，他引：1
研究比较了黄酒、姜葱蒜以及姜葱蒜-黄酒复合脱腥保鲜液对于新鲜草鱼鱼肉的脱腥效果,并选用脱腥效果最好的姜葱蒜-黄酒复合脱腥保鲜液应用于新鲜草鱼鱼肉的保鲜.通过测定货架期草鱼鱼肉的pH值、挥发性盐基总氮、TBA值、菌落总数以及感官评定,研究了脱腥保鲜液对于草鱼鱼肉货架期品质的影响.结果表明,该复合脱腥保鲜液能够有效地保持鱼肉的货架期品质,延缓鱼肉pH值、TVBN值、TBA值以及菌落总数的升高. 相似文献
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【目的】研究阿维菌素在草鱼体内的药物代谢动力学,为实际生产中阿维菌素的使用提供理论指导。【方法】用初始质量浓度为0.3 μg/L的阿维菌素水溶液药浴草鱼,于给药后0.5,1,2,3,4,6,8,10,12,24,48,72,96,144,216,336,528和576 h采取血浆、肌肉+皮、肝脏、肾脏、鳃等样品,采用高效液相色谱荧光法测定阿维菌素在草鱼血浆中的质量浓度及在组织中的含量,数据经3P97药动学软件分析。【结果】在(26.0±1.0) ℃的水温条件下,阿维菌素单剂量浸泡给药0.3 μg/L,血药经时过程符合二室开放式模型。主要药动学参数如下：分布半衰期(T1/2α)34.2 h,吸收半衰期(T1/2(ka))15.61 h,消除半衰期(T1/2β)163.22 h,药时曲线下面积(AUC)2 486.02 (μg·h)/L,达峰时间(Tpeak)40.75 h,峰质量浓度(Cmax)11.92 μg/L。药后72 h时草鱼肌肉、肝脏、肾脏和鳃中阿维菌素含量均达到最高值,其中肝脏中的含量最高,达到17.8 μg/kg,其后依次为肾脏(12.1 μg/kg)、肌肉(10.7 μg/kg)和鳃组织(5.2 μg/kg),血浆中阿维菌素含量在48 h达到最高(11.2 μg/L)。肝脏、肾脏和鳃组织中阿维菌素含量均呈“双峰”曲线,前两者在144 h时都有第2次吸收高峰,分别为15.0和8.4 μg/kg。【结论】草鱼血浆及各组织中阿维菌素在给药后24 d未检出,考虑到临床应用情况的复杂性及理论值与实测值之间的差距,建议对草鱼单剂量(0.3 μg/L)药浴阿维菌素后的休药期为24 d。 相似文献
20.
[目的]探讨草鱼肠道菌群与草鱼个体大小之间的相关性。[方法]采用DGGE方法对同一批孵化并在同一池塘中养殖的个体大小显著差异的草鱼肠道样品进行分析。[结果]无论大个体草鱼还是小个体草鱼,其肠道优势菌群均为厚壁菌门和变形菌门,但2组草鱼在其肠道菌群的结构和多样性等方面存在差异。大个体草鱼肠道中存在着更多比例的厚壁菌门以及具有纤维素降解能力的细菌,而小个体草鱼肠道中存在较多的潜在致病菌。[结论]草鱼肠道微生物可以作为内在因素影响草鱼的生长性能,这一结果也为进一步开发可促进草鱼生长性能的益生菌奠定了研究基础。 相似文献