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1.
On irrigated agricultural soils from semi-arid and arid regions, ammonia (NH3) volatilization and nitrous oxide (N2O) emission can be a considerable source of N losses. This study was designed to test the capture of 15N loss as NH3 and N2O from previous and recent manure application using a sandy, calcareous soil from Oman amended one or two times with 15N labeled manure to elucidate microbial turnover processes under laboratory conditions. The system allowed to detect 15N enrichments in evolved N2O-N and NH3-N of up to 17% and 9%, respectively, and total N, K2SO4 extractable N and microbial N pools from previous and recent 15N labeled manure applications of up to 7%, 8%, and 15%. One time manured soil had higher cumulative N2O-N emissions (141 µg kg?1) than repeatedly manured soil with 43 µg kg?1 of which only 22% derived from recent manure application indicating a priming effect.  相似文献   

2.
The effects of repeated soil drying and rewetting on microbial biomass N (Nbio) and mineral N (Nmin) were measured in incubation experiments simulating typical moisture and temperature conditions for soils from temperate climates in the post‐harvest period. After application of in vitro 15N‐labeled fungal biomass to a silty loam, one set of soils was exposed to two drying‐rewetting cycles (treatment DR; 14 days to decrease soil moisture to 20 % water‐holding capacity (WHC) and subsequently 7 days at 60 % WHC). A control set (treatment CM) was kept at constant moisture conditions (60 % WHC) throughout the incubation. Nbio and Nmin as well as the 15N enrichment of these N pools were measured immediately after addition of 15N‐labeled biomass (day 0) and after each change in soil moisture (day 14, 21, 35, 42). Drying and rewetting (DR) resulted in higher Nmin levels compared to CM towards the end of the incubation. Considerable amounts of Nbio were susceptible to mineralization as a result of soil drying (i.e., drying enhanced the turnover of Nbio), and significantly lower Nbio values were found for DR at the end of each drying period. Immediately after biomass incorporation into the soil (day 0), 22 % of the applied 15N was found in the Nmin pool. Some of this 15Nmin must have been derived from dead cells of the applied microbial biomass as only about 80 % of the microbes in the biomass suspension were viable, and only 52 % of the 15Nbio was extractable (using the fumigation‐extraction method). The increase in 15Nmin was higher than for unlabeled Nmin, indicating that added labeled biomass was mineralized with a higher rate than native biomass during the first drying period. Overall, the effect of drying and rewetting on soil N turnover was more pronounced for treatment DR compared to CM during the second drying‐rewetting cycle, resulting in a higher flush of mineralization and lower microbial biomass N levels.  相似文献   

3.
Analysis of manure and soil nitrogen mineralization during incubation   总被引：1，自引：0，他引：1  
Understanding the N-cycling processes that ensue after manuring soil is essential in order to estimate the value of manure as an N fertilizer. A laboratory incubation of manured soil was carried out in order to study N mineralization, gas fluxes, denitrification, and microbial N immobilization after manure application. Four different manures were enclosed in mesh bags to allow for the separate analysis of manure and soil. The soils received 0.15 mg manure N g–1 soil, and the microcosms were incubated aerobically and sampled throughout a 10-week period. Manure addition resulted in initial NH4-N concentrations of 22.1 to 36.6 mg kg–1 in the microcosms. All manured microcosms had net declines in soil mineral N. Denitrification resulted in the loss of 14.7 to 39.2% of the added manure N, and the largest N losses occurred in manures with high NH4-N content. Increased soil microbial biomass N amounted to 6.0 to 8.6% of the added manure N. While the microcosms as a whole had negative N mineralization, all microcosms had positive net nitrification within the manure bags. Gas fluxes of N2O and CO2 increased in all manured soils relative to the controls. Our results show that measurement of microbial biomass N and denitrification is important to understand the fate of manure N upon soil application.  相似文献   

4.
Plots of a tall fescue (Festuca arundinacea) sward in the south coastal region of BC, Canada, were treated with dairy manure slurry or fertilizer at 50 or 100 kg NH4-N ha−1 up to four times per year for six consecutive years; control plots received no manure or fertilizer. The length of fungal hyphae and abundance of bacterial cells were determined by direct counting at 19 sample dates during the fourth (1997), fifth (1998) and sixth (1999) application years. Bacterial abundance was significantly greater in manured soil than in fertilized and untreated soils. In contrast, hyphal length was significantly greater in untreated soil than in manured and fertilized soils. In subplots that ceased to receive manure in 1998, bacterial abundance remained greater through 1998 and 1999 than in previously fertilized plots, indicating that the 4 year cumulative effect of manure was detectable for at least two growing seasons after applications cease. The apparently negative effect of manure and fertilizer on fungal hyphae also appeared to persist through 2 years after applications ceased. Bacterial abundance increased after an initial application of manure for 1 year to previously untreated plots, but not to levels comparable to plots treated with manure continuously from 1994 to 1998.Increases in bacterial abundance, during the one to three week intervals immediately following individual applications of manure, were inconsistent and other factors, such as soil moisture, temperature and perhaps crop phenology appear to have had strong effects on the timing of these microbial responses. Annual means for bacterial abundance and total microbial biomass in the continuous manure treatment were similar for all 3 years. This suggested that the manure-induced increase in microbial biomass probably reached a plateau between one and 3 years after applications commenced. The large bacterial populations along with abundant carbon substrates in manured soil, relative to fertilized soil, were probably capable of immobilizing influxes of mineral N, explaining the observations that less leaching occured from manured than from fertilized soils.  相似文献   

5.
Summary Fifteen- and forty-year-old cropfields developed from a dry tropical forest were examined for soil organic C and total N and soil microbial C and N. The 15-year-old field had never been manured while the 40-year-old field had been fertilized with farmyard manure every year. The native forest soil was also examined. The results indicated that the native forest soil lost about 57% and 62% organic C and total N, respectively, in the 0–10 cm layer after 15 years of cultivation. The microbial C and N contents of the forest soil were greater than those of the cultivated soils. Application of farmyard manure increased the biomass-C and -N levels in the cultivated soil but the values were still markedly lower than in the forest soil. There was an appreciable seasonal variation in biomass C and N, the values being highest in summer and lowest in the rainy season. During an annual cycle, biomass-C contents varied from 180 to 727 g g–1 and N from 20 to 80 g g–1 dry soil, and both were linearly related. Microbial biomass C represented 1.6%–3.6% of total soil organic C and microbial biomass N represented 1.7% 1–4.4% of soil organic N.  相似文献   

6.
The effect of presubmergence and green manuring on various processes involved in [15N]‐urea transformations were studied in a growth chamber after [15N]‐urea application to floodwater. Presubmergence for 14 days increased urea hydrolysis rates and floodwater pH, resulting in higher NH3 volatilization as compared to without presubmergence. Presubmergence also increased nitrification and subsequent denitrification but lower N assimilation by floodwater algae caused higher gaseous losses. Addition of green manure maintained higher NH4+‐N concentration in floodwater mainly because of lower nitrification rates but resulted in highest NH3 volatilization losses. Although green manure did not affect the KCl extractable NH4+‐N from applied fertilizer, it maintained higher NH4+‐N content due to its decomposition and increased mineralization of organic N. After 32 days about 36.9 % (T1), 23.9 % (T2), and 36.4 % (T3) of the applied urea N was incorporated in the pool of soil organic N in treatments. It was evident that the presubmergence has effected the recovery of applied urea N.  相似文献   

7.
A laboratory soil incubation and a pot experiment with ryegrass were carried out in order to examine the extractability of microbial biomass N by using either 10-mM CaCl2 extraction or the electro-ultrafiltration (EUF) method. The aim of the experiment was to test the hypothesis whether the organic N (Norg) extracted by EUF or CaCl2 from dried soil samples represents a part of the microbial biomass. For the laboratory incubation a 15N-labelled Escherichia coli suspension was mixed with the soil. For the pot experiment a suspension of 15N-labelled bacteria was applied which had previously been isolated from the soil used. Soil samples of both treatments, with and without applied bacterial suspension, were extracted by EUF and CaCl2. The extractability of applied microbial biomass was estimated from the difference in extractable Norg between the two treatments. In addition, the N isotopic composition in the upper plant matter, in the soil, and in organic and inorganic N fractions of EUF and CaCl2 extracts was analysed. Both experiments showed that the applied microbial biomass was highly accessible to mineralization and thus represented potentially mineralizable N. However, this mineralizable N was not extractable by CaCl2 or by the EUF method. It was, therefore, concluded that the organic N released on soil drying and which was thus extractable was derived from the non-biomass soil organic matter. The result suggests that both extraction methods may provide a suitable index for mineralizable N only in cases where the decomposable organic substrates are derived mainly from sources other than the living soil biota.Dedicated to Professor J. C. G. Ottow on the occasion of his 60th birthday  相似文献   

8.
研究结果表明,有机、无机肥施用后,土壤微生物量C、N、P开始增加很快,随着时间的推移,土壤微生物量C又有所降低,但生物量N和P则基本保持稳定。硫铵施入土壤后,微生物对肥料15N的生物固持10天后达到最高峰,以后被固持在体内的15N有一部分被逐渐释放出来,但一个月后仍有17%左右的15N被固持在微生物体内。硫铵与有机肥配合施用时,微生物对硫铵15N固持比例有所增加。有机肥中的15N被微生物固持的比例也较大,在肥料施入20天左右达到最大值,一个月后仍有19-25%存在于微生物体内。硫铵施用一个月后15N损失高达18%,有机肥中的N也有少量被损失。  相似文献   

9.
Summary We studied the build-up and turnover of microbial biomass following the addition of farmyard manure to an unmanured soil and to soils from a long-term experiment in which different levels of farmyard manure had been applied for the last 23 years. The application of farmyard manure at 15–90 t ha-1 to previously unmanured soil increased the microbial biomass during the first 3 months of incubation but a gradual decline occurred with further incubation for up to 12 months. Microbial biomass C was positively correlated with soil organic C and ranged from 1.8% to 2.2% of organic C after 12 months of farmyard manure applications. Biomass turnover increased with the application of farmyard manure, ranging from 0.81 to 0.87 year-1 with various levels of manure. Amendment of soils from the long-term manure experiment with various levels of farmyard manure led to a build-up and decline in biomass C as seen in the unmanured soils, but biomass C was higher in all treatments compared to the corresponding unmanured soil treatments. Biomass turnover was greater compared to the unmanured soil treatments and it decreased with increasing levels of farmyard manure. The average soil respiratory activity increased with increasing levels of farmyard manure, but respiratory activity per unit of biomass C decreased with increasing levels of manure. Enzyme activities were greater in long-term manured soils compared to unmanured soils amended with various levels of manure. There was a significant correlation between biomass C and enzyme activities.  相似文献   

10.
The effects of annual application of rice straw or cow manure compost for 17–20 y on the dynamics of fertilizer N and soil organic N in Gley paddy fields were investigated by using the 15N tracer technique during the rice cropping season. The chloroform fumigation-extraction method was evaluated to determine the properties of soil microbial biomass under submerged field conditions at the tillering stage before mid-summer drainage, with special reference to the fate of applied NH4 +-15N.

The transfer ratios from applied NH4 +-15N to immobilized N in soil and to uptake N by rice during given periods varied with the rice growth stages and were affected by organic matter application. The accumulated amounts of netmineralized soil organic N (net-Mj ), immobilized N (Ij ), and denitrified N (Dj ) during the cropping season were estimated to be 14.0–22.5, 6.3–11.2, and 3.4–5.3 g N m-2, respectively. Values of net-Mj and Ij were larger in the following order: cow manure compost plot > rice straw plot > plot without organic matter application, and their larger increase by the application of cow manure compost contributed to a decrease of the Dj values, as compared with rice straw application.

Values of E N extra extractable soil total N after fumigation, increased following organic matter application, ranging from 2.1 to 5.4 g N m-2. Small residual ratios of applied 15N in the fraction E N at the end of the given period indicated that re-mineralization of newly-assimilated 15N through the easily decomposable fraction of microbial biomass had almost ended. Thus, the applicability to paddy field soils of the chloroform fumigation-extraction method was confirmed.  相似文献   

11.
The availability of C and N to the soil microbial biomass is an important determinant of the rates of soil N transformations. Here, we present evidence that changes in C and N availability affect the 15N natural abundance of the microbial biomass relative to other soil N pools. We analysed the 15N natural abundance signature of the chloroform‐labile, extractable, NO3–, NH4+ and soil total N pools across a cattle manure gradient associated with a water reservoir in semiarid, high‐desert grassland. High levels of C and N in soil total, extractable, NO3–, NH4+ and chloroform‐labile fractions were found close to the reservoir. The δ15N value of chloroform‐labile N was similar to that of extractable (organic + inorganic) N and NO3– at greater C availability close to the reservoir, but was 15N‐enriched relative to these N‐pools at lesser C availability farther away. Possible mechanisms for this variable 15N‐enrichment include isotope fractionation during N assimilation and dissimilation, and changes in substrate use from a less to a more 15N‐enriched substrate with decreasing C availability.  相似文献   

12.
Summary We used 15N technology to investigate N2 fixation by Sesbania speciosa and Sesbania rostrata and its transfer to a lowland rice crop after incorporation of the Sesbania spp. into soil as green manure. During the first 50 days after establishment in November–December 1989, S. speciosa and S. rostrata produced 1126 and 923 kg dry matter ha-1 respectively. They gathered 31 and 23 kg N ha-1 respectively, of which 62%±5% and 55%±3% respectively, came from N2 fixation. Both these species produced a greater biomass during September–October 1989, with S. rostrata producing more than S. speciosa. These results reflected differential responses by the plants to different day lengths at different times of the year. Furthermore, the dry matter yield and %N of 15N-labelled S. speciosa were smaller than those of the unlabelled plants, possibly due to inhibition of N2 fixation in root nodules by the chemical N fertilizers added during labelling. These differences were not so pronounced in the stem-nodulated S. rostrata. The increased grain yield of rice fertilized with N in the form of chemical fertilizer or green manure was a result of an increased number of panicles per hill. The rice crop manured with S. speciosa produced a lower grain yield, with a lower grain weight than that manured with S. rostrata. This was due to a low uptake of soil N by rice manured with S. speciosa. Recovery of N from the green manure in rice straw with S. speciosa was significantly higher than from rice manured with S. rostrata, because of the higher applied N uptake by rice manured with the former.  相似文献   

13.
The response of 8 leguminous cover crops to phosphorus (P) application (7.5 mg P2O5 kg‐1 soil or 15 kg P2O5 ha‐1 to the depth of 15 cm) on soils with variable history was evaluated in a pot trial supplemented with a field experiment in 1993. The soil from a livestock farmer's field showed higher total organic carbon content and extractable cations compared to that from a non‐livestock farmer's field. In the pot trial, P application, on average, increased shoot, root, nodule dry matter and nitrogen (N) accumulation of the legumes by 82%, 45%, 871%, and 900%, respectively, compared to the control. Cajanus cajan, Crotalaria ochroleuca, Centrosema pascuorum, and white‐seeded Mucuna pruriens showed a higher P response than Centrosenza brasilianum and Chamaecrista rotundifolia. The legumes grown on the manured soil showed not only higher biomass and N accumulation, but also higher increase (110% and 117%) in total dry matter and N accumulation because of P application than those grown on the un‐manured soil (27% and 45%). In the field experiment, spreading legume groundcover at 16 weeks after planting was increased by 40% in the un‐manured soil and by 31% in the manured soil. Centrosema brasilimmm even showed a negative response of groundcover to P application. There was little response in erect legume height to P, except for measurements at 6 and 8 weeks after planting, when P increased plant height for Crotaktria on un‐manured soil. Results imply high returns can be expected when P is applied to leguminous cover crops in fairly fertile soil. The relatively low response under the field conditions, compared to pot, suggests caution is needed when P is recommended for legumes grown under environmentally stressed conditions.  相似文献   

14.
Abstract

To determine the relationships between microbial biomass nitrogen (N), nitrate–nitrogen leaching (NO3-N leaching) and N uptake by plants, a field experiment and a soil column experiment were conducted. In the field experiment, microbial biomass N, 0.5 mol L?1 K2SO4 extractable N (extractable N), NO3-N leaching and N uptake by corn were monitored in sawdust compost (SDC: 20 Mg ha?1 containing 158 kg N ha?1 of total N [approximately 50% is easily decomposable organic N]), chemical fertilizer (CF) and no fertilizer (NF) treatments from May 2000 to September 2002. In the soil column experiment, microbial biomass N, extractable N and NO3-N leaching were monitored in soil treated with SDC (20 Mg ha?1) + rice straw (RS) at five different application rates (0, 2.5, 5, 7.5 and 10 Mg ha?1 containing 0, 15, 29, 44 and 59 kg N ha?1) and in soil treated with CF in 2001. Nitrogen was applied as (NH4)2SO4 at rates of 220 kg N ha?1 for SDC and SDC + RS treatments and at a rate of 300 kg N ha?1 for the CF treatment in both experiments. In the field experiment, microbial biomass N in the SDC treatment increased to 147 kg N ha?1 at 7 days after treatment (DAT) and was maintained at 60–70 kg N ha?1 after 30 days. Conversely, microbial biomass N in the CF treatment did not increase significantly. Extractable N in the surface soil increased immediately after treatment, but was found at lower levels in the SDC treatment compared to the CF treatment until 7 DAT. A small amount of NO3-N leaching was observed until 21 DAT and increased markedly from 27 to 42 DAT in the SDC and CF treatments. Cumulative NO3-N leaching in the CF treatment was 146 kg N ha?1, which was equal to half of the applied N, but only 53 kg N ha?1 in the SDC treatment. In contrast, there was no significant difference between N uptake by corn in the SDC and CF treatments. In the soil column experiment, microbial biomass N in the SDC + RS treatment at 7 DAT increased with increased RS application. Conversely, extractable N at 7 DAT and cumulative NO3-N leaching until 42 DAT decreased with increased RS application. In both experiments, microbial biomass N was negatively correlated with extractable N at 7 DAT and cumulative NO3-N leaching until 42 DAT, and extractable N was positively correlated with cumulative NO3-N leaching. We concluded that microbial biomass N formation in the surface soil decreased extractable N and, consequently, contributed to decreasing NO3-N leaching without impacting negatively on N uptake by plants.  相似文献   

15.
Abstract

Laboratory incubation and greenhouse experiments were conducted with two soils having contrasting physico‐chemical characteristics to evaluate nitrogen (N) mineralization, immobilization in soil microbial biomass, and accumulation in Japanese mint (Mentha arvensis L.) using labeled (15NH4)2SO4, applied at 0, 50, and 100 mg#lbkg‐1 soil. Rate of mineralization in soils varied from 0.08 to 2.21 μg#lbg‐1#lbday‐1. Fertilizer application increased the mineralization of native soil N. About 22 to 60% of the applied 15N was recovered in the soil microbial biomass during the growth period of mint (January‐June). Relative contribution of fertilizer 15N towards total N uptake by mint at maturity was 42–54% in soil I and 35 to 55% in soil II. Contribution of soil N towards total N accumulation increased with the doses of 15N application.  相似文献   

16.
Sulfonamides are the second most used antibiotic class in veterinary medicine and applied to livestock to treat bacterial infections. Subsequently, they are spread onto agricultural soils together with the contaminated manure used as fertilizer. Both manure and antibiotics affect the soil microbial community. However, the influence of different liquid manure loads on effects of antibiotics to soil microorganisms is not well understood. Therefore, we performed a microcosm experiment for up to 32 d to clarify whether the function and structure of the soil microbial community is differently affected by interactions of manure and the antibiotic sulfadiazine (SDZ). To this end selected concentrations of pig liquid manure (0, 20, 40, 80 g kg–1) and SDZ (0, 10, 100 mg kg–1) were combined. We hypothesized that incremental manure amendment might reduce the effect of SDZ in soils, due to an increasing sorption capacity of SDZ to organic compounds. Clear dose‐dependent effects of SDZ on microbial biomass and PLFA pattern were determined, and SDZ effects interacted with the liquid manure application rate. Soil microbial biomass increased with incremental liquid manure addition, whereas this effect was absent in the presence of additional SDZ. However, activities of enzymes such as urease and protease were only slightly affected and basal respiration was not affected by SDZ application, while differences mostly depended on the concentration of liquid manure. These results illustrated that the microbial biomass and structural composition react more sensitive to SDZ contamination than functional processes. Furthermore, effects disproportionally increased with incremental liquid manure addition, although extractable amounts of SDZ declined with increasing liquid manure application.  相似文献   

17.
Improving manure management to benefit both agricultural production and the environment requires a thorough understanding of the long‐term effects of applied manure on soil properties. This paper examines the effect of 25 annual solid cattle manure applications on soil organic carbon (OC), total N (TN), and KCl‐extractable NO3‐N and NH4‐N under both non‐irrigated and irrigated conditions. After 25 annual manure applications, OC and TN contents increased significantly with the rate of manure application at the top two sampling depths (0–15 cm and 15–30 cm), and the increases were not affected by the irrigation treatment. The NO3 content increased at all sampling depths with greater increases observed under non‐irrigated conditions, while NH4 content was not affected by manure application rates or the irrigation treatment. The changes in OC and TN at the surface (0–15 cm) and 15–30 cm depth were dependent on the cumulative weight of manure added over the years. The relationships between cumulative manure OC added and soil OC content and between cumulative manure TN added and soil TN content were linear and not affected by the irrigation treatment. For every ton of manure OC added, soil OC increased by 0.181 g kg–1 in the topsoil (0–15 cm). Similarly, for every ton of manure TN added, surface soil TN increased by 0.192 g kg–1. The linear relationship between manure C added and soil C content suggests that the soil had a high capacity for short‐term C sequestration. However, the total amount of NO3‐N in the soil profile (0–150 cm) was affected by both the manure application rates and the irrigation treatment. A large amount of NO3 accumulated in the soil, especially under non‐irrigated conditions. The extremely high level of NO3 in the soil increases the potential risk of surface and groundwater pollution and losses to atmosphere as N2O.  相似文献   

18.
Long‐term applications of inorganic fertilizers and farmyard manure influence organic matter as well as other soil‐quality parameters, but the magnitude of change depends on soil‐climatic conditions. Effects of 22 annual applications (1982–2003) of N, P, and K inorganic fertilizers and farmyard manure (M) on total organic carbon (TOC) and nitrogen (TON), light‐fraction organic C (LFOC) and N (LFON), microbial‐biomass C (MB‐C) and N (MB‐N), total and extractable P, total and exchangeable K, and pH in 0–20 cm soil, nitrate‐N (NO ‐N) in 0–210 cm soil, and N, P, and K balance sheets were determined using a field experiment established in 1982 on a calcareous desert soil (Orthic Anthrosol) at Zhangye, Gansu, China. A rotation of irrigated wheat (Triticum aestivum L.)‐wheat‐corn (Zea mays L.) was used to compare the control, N, NP, NPK, M, MN, MNP, and MNPK treatments. Annual additions of inorganic fertilizers for 22 y increased mass of LFON, MB‐N, total P, extractable P, and exchangeable K in topsoil. This effect was generally enhanced with manure application. Application of manure also increased mass of TOC and MB‐C in soil, and tended to increase LFOC, TON, and MB‐N. There was no noticeable effect of fertilizer and manure application on soil pH. There was a close relationship between some soil‐quality parameters and the amount of C or N in straw that was returned to the soil. The N fertilizer alone resulted in accumulation of large amounts of NO ‐N at the 0–210 cm soil depth, accounting for 6% of the total applied N, but had the lowest recovery of applied N in the crop (34%). Manure alone resulted in higher NO ‐N in the soil profile compared with the control, and the MN treatment had the highest amount of NO ‐N in the soil profile. Application of N in combination with P and/or K fertilizers in both manured and unmanured treatments usually reduced NO ‐N accumulation in the soil profile compared with N alone and increased the N recovery in the crop as much as 66%. The N that was unaccounted for, as a percentage of applied N, was highest in the N‐alone treatment (60%) and lowest in the NPK treatment (30%). In the manure + chemical fertilizer treatments, the unaccounted N ranged from 35% to 43%. Long‐term P fertilization resulted in accumulation of extractable P in the surface soil. Compared to the control, the amount of P in soil‐plant system was surplus in plots that received P as fertilizer and/or manure, and the unaccounted P as percentage of applied P ranged from 64% to 80%. In the no‐manure plots, the unaccounted P decreased from 72% in NP to 64% in NPK treatment from increased P uptake due to balanced fertilization. Compared to the control, the amount of K in soil‐plant system was deficit in NPK treatment, i.e., the recovery of K in soil + plant was more than the amount of applied K. In manure treatments, the recovery of applied K in crop increased from 26% in M to 61% in MNPK treatment, but the unaccounted K decreased from 72% in M to 37% in MNPK treatment. The findings indicated that integrated application of N, P, and K fertilizers and manure is an important strategy to maintain or increase soil organic C and N, improve soil fertility, maintain nutrients balance, and minimize damage to the environment, while also improving crop yield.  相似文献   

19.
 Nitrogen (N) fluxes through the major plant pools of an alder (Alnus sinuta)-sweet corn (Zea mays) alley cropping system were determined over the course of two cropping seasons. Alder trees were injected with 15NO3–N to directly follow the flow of N between alder and corn. The contribution of the above- and below-ground tree N to corn was determined by exchanging the labeled above-ground prunings (green manure) with those from unlabeled plots. During the first growing season after coppicing of the injected alders, 18% of the alder 15N was taken up by the corn with 12% coming from the above-ground prunings. Of the 15N remaining in the tree/stump following coppicing, the majority was recovered by corn plants within the rows next to the labeled trees during the first growing season. Earlier recovery of 15N by corn in the labeled root plots compared to the labeled pruning plots indicated the importance of root turnover in supplying N to corn, especially following coppicing. By the end of the first and second growing seasons, 34% and 38% of the 15N initially present in prunings was recovered in corn plants, respectively. Approximately 80% of the total injected 15N was found in the soil during the second growing season; however, the turnover of above- and below-ground alder components supplied only 3–4% of the N required by corn during the year of green manure application. Thus, most of the corn N demand was met by mineralization of residual soil N within the 2 years of coppicing and green manure additions. Continued internal cycling of tree N and movement of soil N into more labile pools would presumably allow more alder N to become available over time. The synchronization between N mineralization from the hedgerow green manure components and nutrient uptake of the alley crop remains a major challenge in alley cropping and other green manure systems. Received: 9 April 1999  相似文献   

20.
A greenhouse rhizobox experiment was carried out to investigate the fate and turnover of 13C‐ and 15N‐labeled rhizodeposits within a rhizosphere gradient from 0–8 mm distance to the roots of wheat. Rhizosphere soil layers from 0–1, 1–2, 2–3, 3–4, 4–6, and 6–8 mm distance to separated roots were investigated in an incubation experiment (42 d, 15°C) for changes in total C and N and that derived from rhizodeposition in total soil, in soil microbial biomass, and in the 0.05 M K2SO4–extractable soil fraction. CO2‐C respiration in total and that derived from rhizodeposition were measured from the incubated rhizosphere soil samples. Rhizodeposition C was detected in rhizosphere soil up to 4–6 mm distance from the separated roots. Rhizodeposition N was only detected in the rhizosphere soils up to 3–4 mm distance from the roots. Microbial biomass C and N was increased with increasing proximity to the separated roots. Beside 13C and 15N derived from rhizodeposits, unlabeled soil C and N (native SOM) were incorporated into the growing microbial biomass towards the roots, indicating a distinct acceleration of soil organic matter (SOM) decomposition and N immobilization into the growing microbial biomass, even under the competition of plant growth. During the soil incubation, microbial biomass C and N decreased in all samples. Any decrease in microbial biomass C and N in the incubated rhizosphere soil layers is attributed mainly to a decrease of unlabeled (native) C and N, whereas the main portion of previously incorporated rhizodeposition C and N during the plant growth period remained immobilized in the microbial biomass during the incubation. Mineralization of native SOM C and N was enhanced within the entire investigated rhizosphere gradient. The results indicate complex interactions between substrate input derived from rhizodeposition, microbial growth, and accelerated C and N turnover, including the decomposition of native SOM (i.e., rhizosphere priming effects) at a high spatial resolution from the roots.  相似文献   

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