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1.
Five isonitrogenous and isocaloric diets were fed to juvenile cobia, to assess the relative contribution of different proteins (fish meal, soybean meal, corn gluten and beer yeast) to the growth of cobia. The dietary effects on nitrogen and carbon turnover and on the isotopic diet‐consumer discrimination factors (Δ15N and Δ13C) were also assessed. Growth results showed that the final body weight, growth rate, feed conversion ratio and protein efficiency ratio of cobia fed diets with alternative protein were significantly lower (< 0.05) than cobia fed diet formulated with 100% fish meal. The estimated half‐lives of nitrogen and carbon ranged between 9–11 days and 6–8 days, respectively, with significant differences among treatments (< 0.05). Δ15N ranged between 0.0–1.2‰ and ?0.1–1.6‰ in whole fish and muscle and Δ13C ranged between 3.8–5.1‰ and 4.0–5.1‰ in whole fish and muscle respectively. Diets were formulated with low levels of dietary nitrogen (10%) supplied by alternative protein sources substituting fish meal. The relative contributions of the dietary nitrogen supplied from these sources to the growth of whole fish and muscle tissue ranged between 4.9–5.2% and 5.9–7.7% respectively. Results indicated that growth accounted for the majority of observed isotopic change in animals under all treatments. In whole animals and muscle tissue, isotopic change due to metabolism occurred faster for carbon stable isotopes than for nitrogen stable isotopes. Cobia fed diets formulated with alternative proteins showed reduced nitrogen turnover rate and increased Δ15N.  相似文献   

2.
The potential of using rendered animal protein ingredients, poultry by‐products meal (PBM), meat and bone meal (MBM), and feather meal (FM), to replace fish meal in diets for malabar grouper, Epinephelus malabaricus, was evaluated in a 10‐week net pen experiment. Triplicate groups of fish (initial body weight 50.2 g) were fed eight isonitrogenous and isocaloric diets formulated to contain 52% crude protein and 9% crude lipid. The control diet contained 50% herring meal, whereas in the remaining seven diets, PBM was incorporated at 11.9 (PM1), 23.8 (PM2), and 35.7% (PM3) to replace 25, 50, and 75% of the fish meal; MBM was incorporated at 14.5 (MM1) and 29.0% (MM2) to replace 25 and 50% of the fish meal; and FM was incorporated at 9.4 (FM1) and 18.8% (FM2) to replace 25 and 50% of the fish meal. A raw fish (RF) diet was used as comparison to assess growth performance of fish fed the formulated diets. Feed intake was lower in fish fed the diets PM3 and FM2 than fish fed the control diet. There were no significant differences in weight gain (WG), final body weight (FBW), nitrogen retention efficiency (NRE), energy retention efficiency (ERE), and total nitrogen waste output (TNW) between fish fed the control diet and the diets PM1, PM2, PM3, MM1, MM2, and FM1. Fish fed the diet FM2 had lower WG, FBW, NRE, and ERE but higher TNW than that of fish fed the control diet. Feed conversion ratio (FCR) was higher in fish fed the diets MM2, FM1, and FM2 than fish fed the control diet. At the end of the experiment, there were no significant differences in whole‐body content of moisture, crude protein, and crude lipid among fish fed the formulated diets. WG, FBW, and TNW of fish fed the diet RF were higher, while FCR and NRE were lower than that of fish fed the control diet. No significant differences were found in feed intake, ERE, and whole‐body composition between fish fed the diet RF and the control diet. Results of the present study suggest that dietary fish meal level for malabar grouper can be lowered from 50 to 38% by incorporating PBM, MBM, or FM.  相似文献   

3.
Three studies were conducted with juvenile cobia, Rachycentron canadum: (Study 1) a 10‐week feeding trial within floating net cages to test the nutritional efficacy of different dietary feeding regimes (trash‐fish control diet, a semimoist diet, an in‐house dry formulated diet, and a commercial cobia feed); (Study 2) a 10‐week feeding trial within an indoor water‐recirculating tank‐based system to test the nutritional efficiency of different potential dietary fishmeal replacers (poultry byproduct meal, soy protein concentrate, feather meal), a diet without taurine supplementation, and a commercial cobia feed; and (Study 3) estimation of the essential amino acid (EAA) requirements of cobia based on EAA whole‐body composition in fast‐growing cobia fed a trash‐fish‐based diet. Fish performance in terms of growth and feed efficiency was the greatest within the outdoor net‐cage feeding trial, with fish fed the control trash‐fish‐based diet exhibiting the best performance. Although fish growth was poorer within the indoor feeding trials, fish performance was similar for most diets, with apparent crude protein digestibility coefficients of over 75% being obtained in all experimental diets in both feeding trials. The estimated EAA requirements of cobia obtained during this study were similar to those reported for other similar marine carnivorous fish species.  相似文献   

4.
The effect of fish meal (FM) substitution with fermented soybean meal (FSBM) in the diets of the carnivorous marine fish, black sea bream, Acanthopagrus schlegelii, was investigated. An 8‐wk feeding trial was conducted with black sea bream (11.82 ± 0.32 g; mean initial weight) in indoor flow‐through fiberglass tanks (25 fish per tank). Six isonitrogenous and isoenergetic diets were formulated, in which FM was replaced by FSBM at 0% (control diet), 10% (FSBM10), 20% (FSBM20), 30% (FSBM30), 40% (FSBM40), or 50% (FSBM50), respectively. Each diet was fed to triplicate groups of fish twice daily to apparent satiation. The results showed that there was no difference in survival of black sea bream during the feeding trial. Fish fed the FSBM10 or FSBM20 diet showed comparable growth performance compared with fish fed the control diet (P > 0.05), whereas more than 30% replacement of FM adversely affected weight gain and specific growth rate (P < 0.05). Feed intake was significantly lower for fish fed the FSBM50 diet compared with fish fed the control diet. Feed conversion ratio (FCR) tended to increase with increasing dietary FSBM with the poorest FCR observed for fish fed the FSBM50 diet. Protein efficiency ratio and protein productive values showed similar patterns. Apparent digestibility of nutrients significantly decreased with increasing dietary FSBM level. With the exception of protein content, no significant differences in whole body and dorsal muscle composition were observed in fish fed the various diets. Fish fed the FSBM50 diet had significantly lower intraperitoneal ratio than fish fed the control or FSBM10 diet. Hepatosomatic index and condition factor were unaffected by dietary treatments. This study showed that up to 20% of dietary FM protein could be replaced by FSBM protein in the diets of juvenile black sea bream.  相似文献   

5.
An 8‐week feeding trial was conducted to evaluate the effects of replacing fish meal (FM) with soybean meal (SBM) and peanut meal (PM) on growth, feed utilization, body composition and haemolymph indexes of juvenile white shrimp Litopenaeus vannamei, Boone. Five diets were formulated: a control diet (FM30) containing 30% fish meal and four other diets (FM20, FM15, FM10 and FM5) in which protein from fish meal was substituted by protein from SBM and PM. The dietary amino acids of diets FM20, FM15, FM10 and FM5 were equal to those of the diet FM30 by adding crystalline amino acids (lysine and methionine). Each diet was randomly assigned to triplicate groups of 30 shrimps (initial weight = 0.48 g), each three times daily. The results indicated that shrimp fed the diets FM15, FM10 and FM5 had poor growth performance and feed utilization compared with shrimp fed the control diet. No difference was observed in feed intake, survival and body composition among dietary treatments. The plasma total cholesterol level of shrimp and the digestibility of dry matter, protein and energy contained in the diets decreased significantly with increasing PM and SBM inclusion levels. Results of this study suggested that fish meal can be reduced from 300 to 200 g kg?1 when replaced by a mixture of SBM and PM.  相似文献   

6.
A 12‐week feeding trial was carried out in concrete tanks to examine complete and partial replacement (75%) of fish meal (FM) with poultry by‐product meal (PBM), meat and bone meal (MBM) and soybean meal (SBM) in practical feeds for African catfish Clarias gariepinus. Triplicate groups of fish (initial body weight ranged from 90.33 to 93.93 g fish−1) were fed seven isonitrogenous and isocaloric diets of 20% digestible protein and 300 kcal 100 g−1 of digestible energy. The control contained 25% herring meal, whereas in the other six diets, PBM, MBM and SBM replaced 75% or 100% of the FM. Final body weight (FBW) and specific growth rate (SGR) of the fish fed diets containing PBM (75% and 100%), SBM (75% and 100%) and MBM (75%) were all higher, but not significantly different than those for fish fed the control diet. Replacing 100% of the FM by MBM significantly lowered FBW and SGR. Concerning whole body composition, there were no significant differences in ash and gross energy content of whole‐body among fish; fish fed diets containing PBM‐100% recorded significantly lower protein content compared with the control diet, while fish fed diet SBM‐100% recorded significantly lower moisture content compared with the control diet. Also fish fed diets SBM‐100% and PBM‐75% recorded higher lipid and gross energy contents compared with the control diet. The study revealed that satisfactory growth and feed utilization responses could be achieved through the replacement of FM by PBM, SBM and MBM in the diet of African catfish.  相似文献   

7.
Replacement of fish meal (FM) protein with dehulled and solvent‐extracted plant by‐products, soybean meal, cottonseed meal, sunflower meal, and linseed meal was tested in diets for juvenile Nile tilapia, Oreochromis niloticus. Fish averaging (±SD) 14.2 ± 2.9 g were divided into 18 groups and fed for 6 mo on pelleted feed containing each of the plant protein meal supplemented with Yucca schidigera powder extract at 750 mg/kg. Methionine (1%) and lysine (0.5%) were added to each diet except the control diet (FMC), while diet FMC + Y was supplemented with yucca only. Three groups of fish were fed each of six isonitrogenous (25% crude protein) and isocaloric (4.3 kcal/g) diets replacing 100% of FM protein and performance compared against a nutritionally balanced control and a commercial tilapia feed. After 6 mo of feeding, the fish fed plant protein diets supplemented with yucca exhibited growth performance not differing significantly from that of fish fed FMC + Y, while differing significantly from the control FMC and diet linseed meal (LSM). The highest apparent protein digestibility coefficient was observed for diets treated with yucca, which was significantly higher than that observed for the control diet FMC. No significant differences were found in whole‐body moisture of fish fed different experimental diets. An increase in the whole‐body protein content was observed in fish fed diets supplemented with yucca, which was significantly different from that of the diet FMC. The whole‐body fat content of Nile tilapia was low and showed significant differences among treatments supplemented with yucca compared with the control diet FMC. The whole‐body energy content showed the same trend as whole‐body fat content.  相似文献   

8.
The supplemental effect of Antarctic krill meal (KM) into a low fish meal (FM) diet was evaluated for olive flounder (Paralichthys olivaceus). A 56% FM‐based diet was regarded as a high FM inclusion diet (HFM), and a low‐FM diet (LFM) was prepared by replacing 50% FM from the HFM. Four other diets were prepared by supplementing 3%, 6%, 9% and 12% KM into the LFM diet gradually replacing soy protein concentrate and tankage meal (designated as KM3, KM6, KM9 and KM12 respectively). Quadruplicate groups of fish were fed one of the diets for 12 weeks. The growth performance and feed utilization efficiency were improved by the dietary KM supplementation. Digestibility of dietary protein and dry matter was increased by the KM3‐9 diets. Haematocrit and haemoglobin were increased by KM supplementation. The innate immunity and antioxidant capacity assessed by Ig, antiprotease, lysozyme, GPx and SOD and the condition factor of fish were significantly increased by KM3‐9 diets. Moreover, goblet cell counts, villi length and fillet yield of fish were significantly improved by all the KM‐containing diets (KM3‐12). A 25‐day‐long challenge test with the Edwardsiella tarda pathogen showed that the cumulative mortality was higher in fish fed the LFM diet than in fish fed the HFM or KM‐supplemented diets. The results indicate that dietary KM supplementation in a LFM diet can increase growth performance and feed utilization efficiency, diet digestibility, intestinal development and functions, innate immunity and disease resistance of olive flounder. The recommended level of KM inclusion in a LFM diet seems to be 6.6% according to quadratic regression analysis.  相似文献   

9.
A 16‐week feeding experiment was conducted to study the feasibility of using broad bean meal (BBM) as a replacement for fish meal (FM) for Nile tilapia Oreochromis niloticus fry, initial average weight 1.9±0.18 g. FM (50% of the diet) was used as sole sources of animal protein in the control diet 1. The replacement levels of BBM in diets (2–5) were 25%, 50%, 75% and 100% of the FM. Methionine (1%) and lysine (0.5%) were added to each diet except the control diet. Three groups of fish were fed each of five isonitrogenous (31.2% CP) and isocaloric (20.1 kJ g?1), and performance was compared against a nutritionally balanced control diet at the end of the experiment. Nile tilapia fed the diet containing 50% BBM exhibited comparable growth with those fed the FM‐based control diet. Digestibility of protein, energy and lipid decreased with increasing levels of BBM above 50% of total replacement FM into the diet. Incorporation of BBM in the diets significantly affected the moisture, fat and energy of whole fish body. These results suggest that BBM can replace 50% of the FM in diet for Nile tilapia fry, without adverse effects on fish performance.  相似文献   

10.
The main objectives of this study were to evaluate the effect of partial and total replacement of fish meal (FM) protein by cow pea seed meal (CPSM) protein in practical diets on growth performance, feed utilization, and body composition of Nile tilapia, Oreochromis niloticus (L.). Fish of an average initial weight of 4.6 ± 0.2 g were stocked in 15 glass aquariums (80 L each) at a rate of 15 fish per aquarium. FM protein (30% of the diet) was used as the sole source of animal protein in the control diet. Percent replacement of FM by CPSM on the basis of crude protein were as follows: 0% (control diet A), 25% (diet B), 50% (diet C), 75% (diet D), and 100% (diet E). Diets were fed to fish at a rate of 4% of the total fish biomass daily, for a period of 16 wk. The results of this study revealed that the fish fed control diet A (100% FM) had the best average final body weight, specific growth rate (SGR %/d), weight gain (g/fish), weight gain %, while the poorest results for all parameters were obtained with fish fed diet E (100% CPSM). The same parameters of fish fed diets B (25% CPSM) and C (50% CPSM) were not significantly different (P > 0.05) from those of fish fed the control diet A. Feed utilization parameters of fish fed diets A, B, C, and D were better than for diet E. Proximate composition of whole‐body moisture and ash contents were not significantly different (P > 0.05) among all experimental diets and control diet. Whole‐body protein contents for fish fed diets B and C were superior to the control diet A. Incorporation of CPSM in the diets significantly increased whole‐body fat content. Incorporation of CPSM in the diets significantly decreased apparent digestibility coefficient of crude protein crude fat and energy. Diets B and C were not significantly different from control diet A. Therefore, these findings suggest that up to 50% of FM protein can be replaced by CPSM protein in Nile tilapia diets without any adverse effects on growth performance, feed utilization, body composition, and digestibility.  相似文献   

11.
Development of efficient cost‐effective diets is a critical component in the refinement of production technologies for the largemouth bass, Micropterus salmoides (LMB). One of the first steps in reducing feed costs can be to decrease the amount of fish meal in the diet. The objective of this study was to evaluate reduced levels of fish meal, and a least‐cost formulation diet, for second year growout of LMB under practical pond conditions. Twelve 0.04‐ha ponds were stocked with juvenile LMB (210.1 ± 3.3 g) at a stocking density of 8650 fish/ha (350 fish/pond). Each pond was randomly assigned one of the four dietary treatments with three replicate ponds per treatment. The three experimental diets contained varying levels of fish meal. Diets FM‐45, FM‐24, and FM‐8 contained 45, 23.5, and 8% fish meal, respectively. In diets FM‐24 and FM‐8, fish meal was replaced by varying levels of poultry by‐product meal, soybean meal, and blood meal. The fourth diet was a commercial salmonid diet widely used as a LMB growout feed (Nelson and Sons, Inc., Silvercup TM , Steelhead, Murray, UT, USA). This diet served as a commercial control (CC) and contained 46% crude protein. The experimental diets were formulated to be isonitrogenous and isocaloric with the CC diet and were fed once daily to apparent satiation for 180 d. At harvest, there were no significant differences between treatments ( P > 0.05 ) in terms of survival, which averaged 95% overall. Mean weights of fish fed the three experimental diets FM‐45, FM‐24 and FM‐8 were not significantly different ( P > 0.05 ) and averaged 518, 546, and 529 g, respectively, but were all significantly greater ( P≤ 0.05 ) than those fed the CC (488 g). Feed conversion ratio (FCR) of fish fed the FM‐45 and FM‐8 diets (1.43 and 1.46, respectively) was significantly greater ( P≤ 0.05 ) than those fed the FM‐24 diet (1.34). The FCR of fish fed the CC diet (1.39) was not significantly different ( P > 0.05 ) from fish fed other diets. Feed cost per unit of weight gain ($US/kg) was significantly lower ( P≤ 0.05 ) in fish fed the FM‐24 and FM‐8 diets ($0.73 and $0.72/kg, respectively) than in fish fed other diets. Feed cost per unit gain of fish fed the FM‐45 diet ($0.83/kg) was significantly lower ( P≤ 0.05 ) than those fed the CC diet ($1.04/kg). There were no significant differences ( P > 0.05 ) in dress‐out percentages or proximate composition among fish fed the four diets. This study indicates that fish meal levels in feeds used for the second year growout of LMB can be reduced to ≥ 8% of the formulation without reducing survival or growth and without negatively impacting body composition.  相似文献   

12.
A plant protein mixture (PPM) was tested to replace fish meal (FM) in diets for juvenile Nile tilapia, Oreochromis niloticus. Fish averaging (±SD) 3.7±0.14 g were divided into 15 groups. Three groups were fed each of five isonitrogenous (33.6%) and isocaloric (4.7 kcal g?1) diets replacing 0%, 25%, 50%, 75% and 100% of the FM protein with similar percentages of PPM (PPM0, PPM25, PPM50, PPM75 or PPM100 respectively). The PPM consisted of 25% soybean meal, 25% cottonseed meal, 25% sunflower meal and 25% linseed meal, and 0.5% of both methionine and lysine were added to each diet except for the control. After 16 weeks of feeding, the fish fed diets PPM75 and PPM100 exhibited growth performance not differing significantly from the fish fed control diet. PPM substitution of up to 75% of the FM protein did not result in differences in the apparent protein digestibility compared with the control, whereas in the PPM100 group digestibility was significantly lower than in the other groups, except for fish fed the PPM75 diet. The incorporation of PPM in diets did not significantly affect whole‐body dry matter, protein, fat or energy compared with the control. The cost–benefit analyses of the test diets indicated that the PPM diets were economically superior to FM. The protein from PPM can completely replace the FM protein in the diets for Nile tilapia, based on the results of this study.  相似文献   

13.
With the increasing emphasis to replace fish meal (FM) with less expensive protein sources in aquaculture diets without reducing weight gains, an 8-wk feeding trial was conducted with juvenile (15 g) sunshine bass Morone chrysops×M. saxatilis) to evaluate growth and body composition when fed diets with different levels of FM (0, 7.5, 15, and 30%). Six practical floating diets were formulated to contain 40% protein and similar energy levels, with various percentages of FM, meat-and-bone meal (MBM), soybean meal (SBM), poultry by-product meal (PBM), and/or distillers grains with solubles (DGS). Ten fish were stocked into each of 24 110-L aquaria and were fed twice daily ad libitum (0730 and 1600 h). At the conclusion of the feeding trial, final weights of fish fed diet 2 (0% FM, 29% SBM, 29% MBM, and 10% DGS), diet 3 (0% FM, 32% SBM, and 28% PBM), diet 5 (15% FM and 44% SBM), and diet 6 (30% FM and 26% SBM) were not significantly different (P > 0.05) and averaged 72 g. However, final weights of sunshine bass fed diet 1 (0% FM, 30% SBM, and 31% MBM) and diet 4 (7.5% FM and 54% SBM) were significantly lower and averaged 55 g. Specific growth rate (SGR) of sunshine bass fed diet 4 was significantly lower (2.14) than fish fed diet 2 (2.70), diet 3 (2.80), diet 5 (2.68), and diet 6 (2.84), while feed conversion ratio (FCR) of fish fed diet 4 was significantly higher than sunshine bass fed diets 2, 3, 5, and 6. Carcass (fish were decapitated) composition of sunshine bass fed diet 4 had a significantly higher percentage of moisture (70%) and protein (54% on a dry-matter basis) than fish fed all other diets. Percentage lipid was similar among fish fed all diets and averaged 41% (dry-matter basis). Results from the present study indicate that diets in which all of the FM is replaced with a combination of animal- and plant-source proteins can be fed to sunshine bass without adverse effects on weight gain, growth rate, and body composition. Further feeding trials are needed to refine diet formulations used in the present study and should be conducted in aquaria and ponds.  相似文献   

14.
The potential of Bacillus subtilis E20‐fermented soybean meal (FSBM) as a partial alternative component of fish meal (FM) in fed diets of orange‐spotted grouper (Epinephelus coioides) was evaluated in this study. An FM‐based diet and seven diets containing 10%, 20% and 30% and 10%, 20%, 30% and 40% of FM replaced by soybean meal (SBM) and FSBM, respectively, were fed to grouper for 84 days to evaluate possible substitution levels of FM by tracking growth performance, digestive enzyme activity and morphological changes in the liver and distal intestine. No significant differences in survival and muscle composition of grouper were found between controls and treatments. Growth performance and feed efficiency of fish fed diets with FM replaced by FSBM up to 30% were not significantly different from controls, whereas significantly decreased growth performance and feed efficiency occurred with diets containing >20% of SBM. Based on the feed efficiency, the maximum substituted levels of FM by SBM and FSBM in grouper diets were 18.36% and 29.32%, respectively, based on broken‐line analyses. Histopathological changes in the liver and distal intestine, and significantly lower activity levels of digestive enzymes, including pepsin in the stomach and trypsin, chymotrypsin, amylase and lipase in the distal intestine, were found in fish fed a diet containing 30% of FM replaced by SBM. However, these parameters were improved by the substitution of FSBM. It is therefore believed that FSBM has great potential to be used as a protein source in grouper diets in partial replacement of FM.  相似文献   

15.
ABSTRACT

This study was conducted as a trial of using dry whey meal (DWM) as a substitute for fish meal (FM) in practical diets for Nile tilapia, Oreochromis niloticus. Triplicate fish groups were fed on five isonitrogenous (30.2%) and isolipidic (6.9%) diets. The control diet (D1) used FM as the sole protein source. In the other four diets (D2–D5), FM protein was substituted by 25, 50, 75, or 100% DWM. Fish (3.5 ± 0.1 g) were stocked at a rate of 20 fish per 100-L aquarium and fed one of the tested diets up to satiation twice daily for 12 weeks. Fish growth, feed utilization, protein efficiency ratio, apparent protein utilization, and energy utilization for fish fed DWM diets up to 75% FM (D2–D4) tended to be higher but were not statistically different than the control diet. No significant effect of diet was found in whole-body moisture, crude protein, and total ash contents. Whole-body lipid content in fish fed the 100% DWM (D5) diet was significantly higher than that for fish fed the control diet. The optimal replacement level of FM by DWM was estimated by second-order polynomial regression to be 62.5%.  相似文献   

16.
Nile tilapia (1.20 g) were fed for 56 days in five treatments (FM100 containing only fish meal [FM] as a protein source; soybean meal (SBM) replace 25%, 50%, 75% and 100% of FM protein in the diets of FM75, FM50, FM25 and FM0, respectively) to assess the effects of dietary protein sources on growth, turnover half‐life of nitrogen and carbon stable isotopes (δ13C and δ15N) in muscle, isotope discrimination factors (Δ15N and Δ13C), and the relative contribution of protein sources to muscle growth. Results showed that the final body weight, weight gain and specific growth rate of fish fed diet FM25 were significantly higher than other treatments (p < .05). By day 35, fish under all treatments reached isotopic equilibrium, and the equilibrium values consistently increase as a function of stable isotope values in diets. Growth accounted for most isotopic changes in muscle and resulted in significantly shorter half‐lives of nitrogen and carbon in FM25 (p < .05). The Δ15N of FM25 and Δ13C of FM0 were significantly lower than other treatments (p < .05). The proportional contributions of SBM protein were significantly higher than their respective levels in FM75 and FM25 (p < .05), indicating a preferential incorporation of nitrogen from the SBM in muscle tissue.  相似文献   

17.
In a 4‐week experiment, 15 cannulated rainbow trout were fed three diets based on fish meal (FM), Saccharomyces cerevisiae yeast (SC) and Wickerhamomyces anomalus and S. cerevisiae yeast mix (WA). Fish were fed daily, and blood samples were collected on day 7 of each week at 0, 3, 6, 12 and 24 hr after feeding. In the final week, fish were exposed to a 1‐min netting stressor. All essential and non‐essential plasma amino acid levels except methionine were similar between fish fed diets FM, SC and WA. Plasma methionine and sarcosine were significantly higher in fish fed diets SC and WA, possibly due to the crystalline methionine level, form or feeding regime. Hydroxy‐proline and 3‐methyl‐histidine were higher in fish fed diet FM, which can be explained by the higher levels present in fish meal compared with yeast. In stressed fish, there were no dietary effects on plasma amino acid levels, but significant increases in taurine and cystathionine were found in stressed compared with unstressed fish. These results demonstrate that yeast‐based diets produce similar plasma amino acid profiles to fish meal and suggest that yeast may be a suitable fish meal replacement in diets for rainbow trout.  相似文献   

18.
We evaluated four fermented protein concentrates (FPCs) as a fish meal replacer in juvenile rainbow trout, Oncorhynchus mykiss. Ten diets were formulated to contain low‐temperature fish meal (LT‐FM), Vietnam fish meal (VT‐FM) and four types of FPCs as fish meal replacers (FPC‐A, B, C & D) at 30% and 50% FM replacement levels. FPC‐A was a mixture of solid‐state fermented soybean meal (SBM) and corn gluten meal (CGM) with Bacillus subtilis; FPC‐B was pretreated acid‐hydrolysed FPC‐A; FPC‐C and FPC‐D were FPC‐A + 2% shrimp soluble extract (SSE) and FPC‐B + 2% SSE, respectively. Triplicate groups of fish (average 15.4 g) were fed one of the experimental diets for 8 weeks. At the end of the feeding trial, fish fed the LT‐FM, FPC‐B, FPC‐C and FPC‐D diets showed significantly higher growth performance at 30% FM replacement than those of fish fed the FPC diets at 50% FM replacement. Fish fed 30FPC‐B, 30FPC‐C and 30FPC‐D diets showed higher weight gain (WG) than fish fed 30FPC‐A diet. Haematological parameters showed no clear trends among the experimental groups. Superoxide dismutase, lysozyme and myeloperoxidase activities were found to be higher in fish fed the LT‐FM, FPC‐A, FPC‐B, FPC‐C and FPC‐D diets at 30% FM replacement than in fish fed the FPC diets at 50% FM replacement. Antinutritional factors such as α‐ and β‐conglycinin, glycinin, trypsin inhibitors or zein were absent in FPC compared with SBM and CGM. Intestinal villi length and muscular thickness were significantly reduced in fish fed the VT‐FM and FPC diets at 50% FM replacement compared to fish fed the LT‐FM and FPC diets at 30% FM replacement. The results show that FPC‐B, FPC‐C, FPC‐D could replace up to 30% of LT‐FM in juvenile rainbow trout.  相似文献   

19.
A 60 days feeding experiment was carried out with Black Sea turbot Psetta maeotica to determine the amount of poultry by‐product meal (PBM) that could replace fish meal (FM) in formulated diets without reducing growth performance. Juvenile Black Sea turbot (initial average weight, 30 g) were fed five isoenergetic (gross energy, 20.5 ± 0.21 kJ g?1 diet) and isonitrogenous diets (protein content, 550 ± 0.35 g kg?1). The control diet used white FM as the sole protein source, the other four diets were prepared to replace FM protein at levels of 25%, 50%, 75% and 100% with PBM. The fish readily accepted all experimental diets and no mortality were recorded during the trial. There were no significant differences in growth performance of turbot (P < 0.05) fed the diets with 25% and 50% replacement levels compared with fish offered the control diet (100% FM), however, final body weight and specific growth rate values in the 50% replacement diet were about 8% lower than those of the control. Total nitrogen excretion in fish fed 50% replacement diet were about 10% higher than the control group, even though these parameters were not found to be statistically different. At the levels of 750 and 1000 g kg?1 of the protein, PBM inclusion caused a severe decrease in growth performance, feed utilization, protein efficiency ratio and per cent nitrogen retention. The results in the present study indicate that up to 25% of FM protein can be replaced by PBM protein without causing reduction in growth performance, nutrient utilization and nitrogen retention.  相似文献   

20.
Alternative protein feedstuffs are potential surrogates for fish meal in the diet of Florida pompano. This study was designed to evaluate the replacement of fish meal with meat and bone meal in a soybean meal‐based diet for Florida pompano. In Trial I (10 wk), the basal diet (FM15, formulated to contain 40% crude protein and 10% lipid) containing 15% fish meal was modified by the isonitrogenous replacement of fish meal with meat and bone meal producing diets with 10 (FM10), 5 (FM05), and 0% (FM0) fish meal. In Trial II (8 wk), the FM0 served as the basal diet (Tau 0) and was modified by the additive supplementation of taurine to produce three test diets containing 0.25 (Tau 0.25), 0.5 (Tau 0.50), and 0.75% (Tau 0.75) taurine. In Trial I, reduced weight gain (220.1%), feed efficiency (0.31), and survival (84.5%) were observed in fish fed the FM0 diet (P < 0.05). In Trial II, the supplementation of taurine improved the growth performance, survival, and protein and energy retention efficiencies of Florida pompano (P < 0.05). These results indicate that the supplementation of taurine was required in these feed formulations and that meat and bone meal is a good alternative ingredient for fish meal in soybean meal‐based diets for Florida pompano.  相似文献   

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