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落叶松不同类型胚性和非胚性愈伤落叶松不同类型胚性和非胚性愈伤 总被引：3，自引：0，他引：3
以华北落叶松继代、增殖培养的不同类型的胚性及非胚性愈伤组织为材料，测定其氨基酸、糖类、酚酸、激素以及离子的含量，结果表明氨基酸含量的差异十分明显，质地硬的非胚性愈伤组织中18种游离氨基酸含量为胚性愈伤组织的2倍以上，经ABA处理后，胚性愈伤组织中多糖及邻苯二酚含量急剧升高；三糖含量与不同基因型密切相关；乙烯释放量在非胚性愈伤组织中高于胚性愈伤组织；金属离子CO 相似文献
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落叶松不同类型胚性和非胚性愈伤组织的生理生化差异 总被引：3，自引：0，他引：3
以华北落叶松替代、增殖培养的不同类型的胚性及非胚性愈伤组织为材料，测定其氨基酸、糖类、酚酸、激素以及离子的含量。结果表明：氨基酸含量的差异十分明显，质地硬的非胚性愈伤组织中18种游离氨基酸含量为胚性愈伤组织的2倍以上，经ABA处理后，胚性愈伤组织中多糖及邻苯二酚含量急剧升高；三糖含量与不同基因型密切相关；乙烯释放量 在非胚性愈伤组织中高于胚性愈伤组织；金属离子CO^ 的含量，胚性愈伤组织是非胚性愈伤组织的12倍，胚性愈伤组织中铵态氮（NH4^ ）与硝态氮（NO3^-)的比值是非胚性愈伤组织中的10倍以上;实验结果为进一步探讨针叶树体细胞胚胎发生的生理生化机理提供依据。 相似文献
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七叶树愈伤组织诱导及过氧化物同工酶的测定 总被引：1，自引：0，他引：1
以七叶树幼嫩叶片为外植体,利用正交试验研究了植物生长调节剂、碳源以及灭菌时间对七叶树叶片愈伤组织诱导的影响,并采用聚丙烯酰胺垂直凝胶电泳法对愈伤组织中过氧化物同工酶进行了测定。试验表明：作为碳源的葡萄糖与蔗糖、麦芽糖相比,更有利于诱导七叶树幼嫩叶片形成愈伤组织,0.1%HgCl2浸泡外植体10 min,起到了较理想的灭菌效果,2.4-D 2.0 mg/L与6-BA 0.4 mg/L为诱导愈伤的最佳浓度组合,测定的愈伤组织过氧化物同工酶谱带与接种前叶片的相比明显增多且不同处理间的条带也不尽相同。 相似文献
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《中南林业科技大学学报(自然科学版)》2020,(10)
【目的】通过组织细胞学观察、扫描电镜观察、生理生化指标测定三种方法为香樟胚性和非胚性愈伤组织的区分提供了可靠的判断依据,建立起愈伤组织的胚性发生能力与生化代谢间的关系,以此为基础可以提高香樟体胚诱导率、优化体胚发生体系。【方法】以长沙7月初采摘的香樟Cinnamomum camphora L.的未成熟合子胚为材料,诱导出四种不同类型的愈伤组织,对其进行组织细胞学观察以及生理生化测定。【结果】研究结果表明:胚性愈伤组织多为白色,表面散布淡黄色颗粒,细胞壁厚质浓、细胞体积较小,排列紧密,在电镜下表面细胞成团分布,大小近似,细胞核大核仁明显,细胞器丰富。而非胚性愈伤组织多为灰色、白色或深黄色,细胞壁薄,内含物少,空泡化明显,细胞多数衰老、破碎,在电镜下非胚性愈伤表面细胞不饱满大部分死亡破裂,且表面存在附着物,内部几乎没有细胞器。且胚性愈伤组织的适应性、抗逆性强于非胚性愈伤组织。对于胚性愈伤组织和非胚性愈伤组织发生的位置来说,非胚性愈伤组织先发生在两片子叶的中间向外部扩增。胚性愈伤组织的发生位置有三种:一种是发生在胚根端,第二种发生在已形成的愈伤组织的外侧,第三种褐化的愈伤组织边缘。【结论】四类愈伤组织中有三类为非胚性愈伤组织,一类为胚性愈伤组织。 相似文献
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以小叶杨（Ｐｏｐｕｌｕｓｓｉｍｏｎｉｉｃａｒｒ．）原生质体培养的愈伤组织为供试材料，用高压波相色谱法（ＨＰＬＣ），测定它们在四种不同分化培养基上四大类内源激素（ＩＡＡ，ＧＡ３，ＣＴＫ，ＡＢＡ）的含量变化，并检定有关Ｎ的生化反应。结果表明，不同分化培养基上的愈伤组织中内源激素含量和种类差异很大。导致细胞分裂素（ＣＴＫ）含量高，种类多以及细胞分裂素与生长素的比值（ＣＴＫ／ＩＡＡ）高的处理，有利于愈伤组织分化；但若愈伤组织中含有内源脱落酸（ＡＢＡ），则抑制分化。测定结果还表明，小叶杨原生质体培养的愈伤组织分化过程中，其硝酸还原酶活力与氨基态Ｎ含量互为消长。分化频率高的愈伤组织中，硝酸还原酶活力低，而氨基态Ｎ含量高． 相似文献
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为了探索桉树抗青枯病树种的无性系快速筛选和繁殖技术,配制了3种类型的培养基,采用组培方法对桉树愈伤组织的抗病性和桉树无性系的快速繁殖方法进行了研究。结果表明:诱导愈伤组织形成的以MS+6-BA0.25mg/L+NAA0.5mg/L培养基最好;对愈伤组织芽的分化,最好的培养基是MS+6-BA0.25mg/L+NAA5mg/L;诱导生根以White+IBA0.5mg/L+2%蔗糖培养基为最佳;分别对桉树愈伤组织刺伤、幼苗伤根、幼苗截顶后接种青枯病,并测定它们的抗病性,其测定结果相同,说明可用桉树愈伤组织筛选抗青枯病树种。 相似文献
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将虎杖P olygonum cusp id a tum不同外植体经不同消毒时间处理后,接种在添加不同激素种类和水平的相同基本培养基上或相同激素种类和水平基本培养基上进行诱导实验,同时对根和根茎芽、叶、韧皮诱导的愈伤组织进行白藜芦醇含量的测定.结果表明:基本培养基以M S较好,外植体叶对激素种类较为敏感,其中适当浓度的NAA诱导愈伤组织比2,4-D的效果好,KT比BA好,添加KT的培养基上诱导愈伤组织比较紧密,有利于分化,在M S+NAA 2 m g/L+KT 0.1 m g/L培养基上诱导愈伤组织较好,根茎芽的诱导率最高,为73%;愈伤组织的生长趋势从接种的第3天开始生长,到21 d时生长达到最高峰,干质量为0.461 2 g,以后生长速度减慢;对不同材料诱导的愈伤组织进行白藜芦醇含量的测定,其中根茎部芽的诱导的愈伤组织中白藜芦醇含量最高,其次是叶和根,最低的为韧皮. 相似文献
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《林业研究》2021,32(2)
The induction and proliferation of embryogenic callus are key steps for large-scale propagation of somatic embryogenesis pathway and long-term preservation of coniferous germplasm.Callus can be induced from immature embryos of Korean pine(Pinus koraiensis Sieb.et Zucc.;Pinaceae) as explants,but there are problems,such as low proliferation efficiency,loss of embryogenicity,poor vigor;thus,best conditions for proliferation and culture of immature embryos of Korean pine are not yet clear.To solve the problems with somatic embryogenesis of Korean pine and determine the best culture conditions for callus induction and proliferation,we varied hormone concentration,subculture cycle of proliferation and other plant growth regulators combinations in media to induce callus formation by megagametophytes of three Korean pine families at different developmental stages,then analyzed the effects on embryogenic callus retention and cell proliferation using a quadratic regression orthogonal rotation design.The results showed that the family origin and collection date of explants significantly affected callus induction(induction rate reached1.67%).Embryogenic maintenance and callus proliferation were best on DCR medium supplemented with 0.25 mg L~(-1)6-benzyl adenine,1 mg L~(-1) naphthaleneacetic acid,30 g L~(-1)sucrose,500 mg L~(-1),L-glutamine,500 mg L~(-1) casein hydrolysis and 6.5 g L~(-1) agar.In addition,the combination of 2,4-dichlorophenoxyacetic acid+6-benzyl adenine also had a better proliferative effect on callus.The effects of different combinations of growth regulators on callus proliferation efficiency were significantly different.Transfer to new medium every 13-15 days not only maintained robust callus vigor,but also yielded a larger proliferation coefficient.The techniques and conditions for embryogenic callus induction and proliferation of Korean determined here will serve as a foundation for establishing a large-scale system for somatic embryogenesis and propagation of Korean pine. 相似文献
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Katsuyoshi Hamada Yuji Tsutsumi Kazuchika Yamauchi Kazuhiko Fukushima Tomoaki Nishida 《Journal of Wood Science》2003,49(4):333-338
Ferulic acid (FA), tetradeuteroferulic acid (DFA), sinapic acid (SA), or heptadeuterosinapic acid (DSA) was exogenously supplied to poplar (Populus alba L.) callus. Administration of FA or SA increased the lignin content of the callus to about twice that of the control callus. Gas chromatographic analysis of the alkali hydrolysate of the cell wall residue revealed that only a trace amount of SA was bound to the cell wall, and the amount of FA was less than 2% of the total callus lignin. Thioacidolysis of the DFA-treated callus indicated that DFA is effectively converted to both coniferyl and sinapyl alcohols and then incorporated into the corresponding lignin. Incorporation of DSA into syringyl lignin or guaiacyl lignin was not observed, but yields of syringyl lignin thioacidolysis products were markedly increased by DSA treatment of the callus. These results suggest that SA may not be a precursor of sinapyl alcohol and syringyl lignin per se, but it may induce or enhance the biosynthesis of syringyl lignin in poplar callus. 相似文献
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种坯处理、不同激素浓度在暗培养条件下对多年生黑麦草愈伤组织诱导的影响 总被引：10，自引：0，他引：10
以多年生黑麦草种子为外随体，研究了在暗培养条件下，不同浓度2，4—D、BA及种胚处理对愈伤组织诱导的影响，试验表明：(1)切胚处理对多年生黑麦草愈伤组织诱导起到很大的促进作用；(2)种胚愈伤组织的诱导宜采用MS 2，4—D 5mg/L的培养基；(3)种子愈伤组织的诱导与种子发芽率无关。 相似文献
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唐巍 《中国林学(英文版)》2001,3(2)
三个基固型的火炬松成熟合子胚被培养在附加 8mg·L-12 ,4 D ,4mg·L-1BA ,4mg·L-1KT ,5 0 0mg·L-1水解酪蛋白和 5 0 0mg·L-1谷氨酰胺的愈伤组织诱导培养基上诱导愈伤组织 .来自于子叶、胚轴和胚根的愈伤组织在附加 1 6mg·L-12 ,4 D ,0 8mg·L-1BA和 0 8mg·L-1KT的愈伤组织增殖培养基上培养 9周后 ,可获得 16 9%的胚性愈伤组织 .通过建立胚性细胞悬浮系和研究ABA、PEG和活性炭对体细胞胚成熟的促进作用 ,优化的体细胞胚胎发生体系被建立 .71棵再生小苗被用于移栽试验 ,2 3棵小苗在田间移栽成活 相似文献
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Single cells were mechanically isolated from leaf-derived callus of mature Juniperus oxycedrus L. These cells divided and gave rise to callus when plated on medium containing growth regulators. Best plating efficiency was obtained on a modified Schenk and Hildebrandt medium supplemented with 0.6 micro M 2,4-dichlorophenoxyacetic acid and 100 mg l(-1) casein hydrolyzate. Although single-cell-derived callus showed poor morphogenic potential, both adventitious shoots and embryogenic tissues differentiated from the callus. We also achieved induction of somatic embryogenesis in leaf explants of mature J. oxycedrus trees cultured in the presence of 6.0 or 10.0 micro M 2,4-dichlorophenoxyacetic acid or picloram. Frequency of embryogenic callus ranged from 6 to 18%; however, under the culture conditions tested, isolated embryos failed to develop into plants. 相似文献
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地涌金莲组织培养中的褐化问题一直是其优良品种选育及规模化生产的技术瓶颈。为解决这一问题,以地涌金莲未成熟雄花为试材,研究了外植体消毒方式、植物生长调节剂种类及配比和不同抗褐化剂对愈伤组织褐化的影响。结果表明,未经消毒的外植体其污染率为0%,且褐化率和褐化指数均显著低于消毒后的外植体;6-BA在地涌金莲愈伤组织诱导中起主要作用,浓度为2 mg·L-1、3 mg·L-1时有利于愈伤组织的诱导,能显著降低褐化指数;添加柠檬酸+抗坏血酸(VC)混合液、表面添加VC均对地涌金莲褐化未起到明显抑制作用,姜汁和阿魏酸对抑制愈伤组织褐化有较好的效果,48.5 mg·L-1的阿魏酸能显著降低愈伤组织的褐化,使愈伤组织分化率达17.9%,较对照的2.9%提高了15%。 相似文献
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Sun Bi-po Zhang Wan-jun Dong Jiang-li Jin Yong-sheng Wang Tao State Key Laboratory of Agro-biotechnology China Agricultural University Beijing P. R. China 《中国林学(英文版)》2006,8(4):25-29
A protocol was discussed for high efficient plant regeneration from seven bluegrass (Poa pratensis L.) cultivars via an indirect callus induction and somatic embryogenesis method. Mature seeds were used as explants for callus initiation. Callus induction and proliferation efficiencies were investigated on NB, modified MS (MMS) and MS media, supplemented with 2.0 mg·L-1 2,4-dichlorophenoxyacetic acid (2,4-D). The MMS medium performed best. Based on the MMS medium, direct and indirect callus induction effects of bluegrass from mature seeds were compared at the range of 1-5 mg·L-1 2,4-D contained in the medium. Under the direct callus induction method, the most suitable 2,4-D concentrations varied among cultivars. Under the indirect callus induction method, a significantly high callus induction frequency (93.33%-98.33%) was obtained and there were barely any statistically significant differences among the tested genetically diverse cultivars. Somatic embryos were promoted on the MMS medium supplemented with 3 mg·L-1 2,4-D, 0.1 mg·L-1 kinetin and 0.8 mg·L-1 CuSO4. Embryogenetic calli developed into plantlets on the MMS medium containing different concentrations of thidiazuron (TDZ), and the differentiation frequencies varied in the range from 20.15% to 77.65%. The 0.25 mg·L-1 TDZ was generally the most suitable concentration for the tested cultivars. 相似文献