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1.
Abstract. Protein and energy maintenance and optimum feed requirements are reported in the catfish, Clarias batrachus (L.), fed a purified diet (40% CP; 1487·1kJ/100g) at 0 to 8% (BW/day) ration levels. Linear (r = 0·985) increase was observed in daily average growth increment up to a ration level of 4% (BW/day), corresponding to 6·03g protein/(kg0·8BW/day) and 224·26 kJ energy/(kg0·8 BW/day). Maintenance requirements, obtained by regressing daily average growth increment to zero, were 0·942 g/(kg0·8 BW/day) for protein and 36·02 kJ/(kg0·8BW/day) for energy. Net gains in muscle protein and energy also depicted linear increase (r = 0·975) with feeding levels up to 6·03g protein/(kg0·8 BW/day) and 224·26kJ energy/(kg0·8 BW/day). On fitting the above data to regression equations, giving the amount of dietary protein and energy required to maintain a constant amount of body protein and energy, values of 1·005g/(kg0·8 BW/day) and 42·11 kJ/(kg0·8BW/day) were obtained for protein and energy respectively. The optimum feeding rate for this species, as evident from specific growth rate and conversion efficiencies, appears to be 3% (BW/day) at 30 ± 2°C. Moisture and lipid contents in muscle were found to be significantly ( P < 0·05) affected by the ration levels.  相似文献   

2.
This study tested the effect of the level of dietary phosphatidylcholine (PC) and its constituent medium-chain fatty acids on microdiet ingestion (μg diet larva−1 h−1) and the absorption rate of the free fatty acid [14C]16:0 (pmole larva−1 h−1) in 15, 20, 21, 25, 26, 30 and 31-day-old gilthead sea bream, Sparus auratus L., larvae. Fish were fed four microdiets (A, B, C and D): microdiet A contained no phospholipid (PL), while microdiet B included 10 g kg−1 Artemia nauplii PL (3.7 g kg−1 PC). Microdiets C and D contained 10 g kg−1 purified saturated PC dimyristoyl (C14:0) and polyunsaturated PC dilinoleoyl (C18:2[cis]−9,12), respectively.
Larvae from one or both of the PC microdiets demonstrated significantly higher ( P < 0.05) ingestion rates (μg diet larva−1 h−1) than the non-PL microdiet control in 15, 21, 22, 25 and 26-day-old larvae and the Artemia PL microdiet in 15, 22 and 26-day-old larvae. However, microdiet ingestion and fatty acid absorption rate appeared to be independent of the associated medium carbon chain saturated or polyunsaturated fatty acid moiety of the PC diets. Apparent absorption, as measured by the retention of radio-labelled [14C]16:0 following 8 h of non-labelled microdiet feeding, was possibly related to feeding.  相似文献   

3.
Abstract. Growth and survival response of Penaeus stylirostris (Stimpson) to fertilization, pelleted feeding and stocking density were analysed for the conditions prevailing on the Baja California peninsula (Mexico). Three 0·25-ha earthen ponds were longitudinally divided in half with mesh structures. One half received inorganic fertilization (trial F) and the other supplementary feeding using a pelletized ration (trial PF). The initial stocking rates were approximately 5, 10 and 15 PL5/m2 in both trials. After 210 days, final mean weights ranged from 15·0 to 5·3g and survival from 48·6 to 17·8%. Growth and survival were analysed by means of a reparameterization of the von Bertalanffy's growth model and the exponential mortality equation. Results indicated that: (1) final mean weight was dependent on density in both trials ( P < 0·01) although growth rate was only affected in trial PF ( P < 0·01); (2) pelleted feeding produced both greater final mean weight and increased growth rate at densities higher than 5 PL/m2 ( P < 0·005); (3) the ratio tail weight/total weight was influenced by density in trial F ( P < 0·015); (4) the mortality equation served to explain differences in survival to a limited extent. An increase in growth rate after 170 days occurring at the intermediate density in trial PF was attributed to an increase in mortality, which allowed the remaining individuals to attain larger sizes. Further research to increase biomass yields should focus on the optimization of pond depth, the supply of vigorous post-larvae and on the possible influences of seasonal variations of planktonic communities of the lagoons on the productivity of ponds.  相似文献   

4.
Abstract. Oxidation treatment of sugarmill waste using duckweed ( Lemna sp.) as part of the system, to reduce effluent nutrient concentrations and biological oxygen demand (BOD), was evaluated during a 6 month milling season in Negros Oriental, Philippines. Mean ammonia concentration in effluent water was reduced from 0·87 to 0·31 mg/l NH3-N and orthophosphate from 0·93 to 0·51 mg/l P2O5, while mean BOD was reduced from 611 to 143mg/t BOD5, after treatment. Seasonal mass fish kills in the adjoining bay no longer occurred during the 3 years following introduction of this treatment. The mean duckweed production (dry weight) was 8–8g/m2/day. Duckweed was harvested from a 1·9ha area of the system and transferred to an adjacent milkfish, Chanos chanos Forskal, farm. Its fertilization effect, in terms of lablab production ( lablab is the biological complex of bluegreen algae, diatoms, bacteria and various animals which forms a mat at the bottom or floats in patches), was evaluated in the milkfish ponds. This was compared with ponds fertilized with either inorganic fertilizers or cow manure, in the traditional way. Lablab growth was significantly increased using duckweed, with ash-free dry weight production averaging 32g/m2/day following fertilization with duckweed compared with 4g/m2/day using inorganic fertilizers. Milkfish net production averaged 320 kg/ha/90-day crop in inorganically fertilized ponds. 545kg/ha/90-day crop for cow manure and 820kg/ha/90-day crop in duckweed-fertilized ponds. The system is described and the benefits of this integrated waste treatment-fish production facility are discussed.  相似文献   

5.
Abstract. The effect of four environmental conditions was investigated upon sperm output in turbot, Scophthalmus maximus (L.), submitted to three different rhythms of stripping. Males kept under a natural light cycle and under a 6-month contracted light programme released a similar sperm output in terms of total volume of semen produced per fish during the experimental period (4·9 ± 0·9ml), mean sperm concentration (29·4 ± 2·8 × 109 spermatozoa/ml) and total sperm number (163·2 ± 40·5 × 109 spermatozoa). Attempts to stimulate spermiation for a second time just after the end of the natural reproduction period resulted in the release of low sperm output (total volume of semen: 1·6 ± 0·4 ml; mean sperm motility: 2 min 36s ± 0 min 47s; mean sperm concentration: 47·6 ± 10·2 × 109 spermatozoa/ml; total sperm number: 84·5 ± 25·3 × 109 spermatozoa). Stripping frequency had no effect on total volume of semen, mean sperm motility and total sperm number. Monthly collection did not modify sperm samples in relation to stripping rank. However, decreasing volume, motility and sperm concentration were observed when males were stripped fortnightly and weekly. During the natural spawning period, the presence of females in the tank enhanced mean sperm motility (from 3 min 27s + 0 min 52s to 6 min 38s ± 1 min 38s).  相似文献   

6.
Abstract. Two grow-out experiments were conducted to evaluate the functional role of chicken manure for Nile tilapia, Oreochromis niloticus (L.). production in central Thailand. Experiment 1 examined the relationship between chicken manure input and net fish yield (NFY). Experiment 2 determined the value of chicken manure in providing tilapia particulate organic carbon, and/or dissolved inorganic carbon (DIC) for stimulating algal productivity. In both experiments supplemental urea and triple superphosphate (TSP) gave all treatments total nitrogen (N) and phosphorus (P) inputs of 28·0kg/ha/week and 7·0kg/ha/week, respectively.
Addition of chicken manure to inorganic fertilization did not enhance production of Nile tilapia. NFY in experiment 1 increased with decreasing manure loading, which corresponded to increasing TSP input. Regression analysis suggested that chicken manure-P was about 10% effective as TSP-P at increasing NFY. NFY was linearly correlated to net primary productivity (r2= 0·62, P < 0·001), which was linearly correlated to total alkalinity (r2= 0·77. P < 0·001).Treatment differences in alkalinity, community respiration or dissolved oxygen concentrations at dawn were not related to manure input. Simple economic comparisons discourage the purchase of chicken manure as a source of soluble N and P for increasing algal productivity in Thailand.  相似文献   

7.
Abstract. A 91-day cage trial was conducted with juvenile seabass, Lates calcarifer (Bloch), and grouper, Epinephelus tauvina (Forskal), to ascertain the capacity of kerosene pressure lamps and fluorescent electric lamps as night-lights above the cages to attract pelagic food organisms into the cage and consequently sustain the survival and growth of the cultured fish. The experiment was conducted using 1×1×1·5m floating cages with three different net mesh sizes (1, 13, and 19mm) and four fish stocking densities (seabass—10, 20,30 and 40/m3; grouper —10/m3). A positive growth response and survival was observed with seabass and to a lesser extent with grouper with increasing net mesh size and decreasing fish stocking density. At the lowest tested density of 10 fish/m3 seabass survival increased from 5·0 to 95·0% and total cage fish biomass increased from −95·1% to +56·9% with an increase in net mesh size from 1 to 19mm over the 91-day culture trial, respectively. The results are discussed in relation to the current commercial marine finfish cage farming practices employed in Indonesia.  相似文献   

8.
Abstract. Six groups of tropical freshwater catfish, Mystus nemurus (Cuvier & Valenciennes)(mean weight. 20·45 ± l·5g), were reared in 0·34m3 fibreglass tanks at different stocking densities (105, 195, 285, 375, 465 and 555 specimens/m3 water) for 84 days. The objective of the study was to determine the effect of various stocking densities on the growth, nutrition, biochemical composition and survival of M. nemurus. The lowest growth rate appeared in fish at the highest density and the highest was observed in fish stocked in moderate density of 285 and 375 fish/m3 water. Fish production was also lower at relatively low stocking densities of 105 and 195 fish/m3. Food conversion ratio (FCR), protein efficiency ratio (PER) and biochemical composition of M. nemurus indicate that there exists an optimum stocking density which lies between 285 and 375 fish/m3.  相似文献   

9.
Abstract. The autccology of chub, Leudscus cephalus (L.), in the Renlina Stream, Greece, was carried out in an effort to obtain information on the age, growth, mortality and reproduction of this species. Fork length increment during the first year of life was 8·9cm, after which it decreased. Life span was short (maximum age 8 years) and the instantaneous mortality rates were higher for the older age-groups. Chub reached sexual maturity at the second year of life for both sexes and spawning occurred from the second fortnight of April into May, at a mean water temperature of 18°C. The relationship between fecundity ( F ) and fork length (1) was F = 0·0797 L3·63 The average density and biomass were 1·06 fish.m−2 and 11·38g.m−2 respectively. The relationship between L and the pharyngeal bone size [gape ( G ), tip ( T ) and shank ( S )] in mm and caudal fin length ( C ) (cm) can be described by the equations: L = 0·8605 + 1·550 G , L = -0·0703 + 1·332 S, L = 1·092 + 1·615 T and L = 1·032 + 2·934 C .  相似文献   

10.
Abstract. The effect on the growth of freshwater angelfish, Pterophyllum scalare (Lichtenstein) (Pisces; Cichlidae), of different densities of population and diet compositions was studied. The results show that increasing the population density from 50 fish/m2 to 200/m2 significantly decreased the rate of growth of fingerlings, and that this effect is correlated to the size of fish. No significant differences were observed in growth between diets containing 37%, 41% or 47% protein. However, the addition of live food (artemia) to the diet significantly raised the growth rate, especially in the higher protein diets.
Body composition shows no changes during the growth periods, with a mean of 64·68% body protein, 35·47% dry weight fat and no significant differences in the percentage of residual ash through the growth period.
The food conversion rate was very low, ranging from 1·15 to 1·31. Total production increased with density, from 4·0g/m2/day (50 fish) to 13·7g/m2/day (200 fish) over a period of 60 days.  相似文献   

11.
Abstract. An enzootic, Australian, atypical strain of Aeromonas salmonicida isolated from diseased goldfish, Carassius auratus (L.), was inoculated into Atlantic salmon, Salmo salar L., brown trout, S. trutta L., rainbow trout, S. gairdneri Richardson, and brook trout, Salvelinus fontinalis (Mitchill), fingerlings by intraperitoneal injection (i.p.) and by bath challenge, the latter with and without prior abrasion of skin. The 10-day LD50 (i.p.) was estimated to be 7·4 × 10-3 colony forming units (cfu) for Atlantic salmon, 3·0 × 10-2 cfu for brown trout, 3·7 × 102 cfu for brook trout and 6·4 × 103 cfu for rainbow trout. Brown, rainbow and brook trout succumbed to bath challenges with between 105–106 cfu/ml, developing ulcers of the skin and septicaemia. The organism was trasmitted from inoculated fish to five of 195 within-tank control fish via water and established a carrier state in one of 14 Atlantic salmon. It was concluded that the organism poses a significant threat to the salmonid farming industry and wild salmonid fisheries in Australia.  相似文献   

12.
Abstract. During three 5-month experiments in Thailand, earthen ponds of approximately 370m2 surface area were stocked with male Nile tilapia, Oreochromis niloticus (L.), fingerlings of 4–12g weight at densities of 0·5 to l·6fish/m2. Stocking and fertilization (with chicken manure, urea and TSP) in triplicated depth treatments of 0·6,1·0 and 1·5m were proportional to pond volume in two experiments (wet and dry seasons) and to pond area in the other (dry season).
Depth showed no direct effect on fish yields of 0·9–6·3t/ha/year, on survival rates of 66 to 98%, nor on final individual weights of 96–313 g/fish. Greater yields were obtained from deeper ponds when they received proportionally greater stocking and fertilizer inputs. Inputs per unit area were the most important factor accounting for yield variation.
Temperature, dissolved N and P, and suspended solids showed little or no relation to depth treatments. Time-averaged chlorophyll concentrations and photosynthetic production of dissolved oxygen were greater in treatments receiving greater inputs of nitrogen per unit pond volume.
Deeper ponds produced the greatest areal yields of fish, when fertilized in proportion to their volumes. Shallow ponds produced fish and dissolved oxygen at least as efficiently per unit input as did deep ponds, which is consistent with models of photosynthesis-depth relations.  相似文献   

13.
Abstract. The immuno dot blot technique is a rapid, sensitive and quantitative method for the diagnosis of infectious pancreatic necrosis virus ( ipnv ) in both infected tissue culture lysates and tissue homogenates of fish. Uninfected tissue culture lysates and fish homogenates do not react in the peroxidase dot blot system after appropriate treatment. The immuno dot blot method takes 4h to complete, and the dot blot membrane can be stored in the dark for a long period. The viral proteins in the infected tissue culture cells could be detected at 3h post-infection if an initial high MOI was used. The minimum dose of ipnv-ab strain which could be detected using homologous serum was 2·5 ng purified ipnv protein/dot. The smallest infectious dose of VR-299, SP, AB, EVE and CY-1 ipnv which could be detected by the immuno dot blot system was 104 to 105 TCID50. When one μg of purified ipnv was used, a 1·6 × 104 dilution of rabbit anti-AB antisera could be detected.  相似文献   

14.
Abstract Two groups of European catfish, Silurus glanis L., fingerlings were infected with channel catfish virus (CCV) by either intraperitoneal injection with 105 TCID50 of CCV, or bathing in water containing 105 TCID50 of CCV per 1·0 ml. The virus was isolated from spleen, intestine and brain of CCV-injected fish at day 1 and the titres ranged from 102·1 to 103·3 TCID50/g. However, the tissue distribution of CCV was irregular and no virus was isolated after day 3 post-exposure. In CCV-bathed fish, the virus was isolated only from the liver of one specimen at day 3 post-exposure. No clinical signs of CCV disease developed in any of the fish. Specimens in each regime from all sampling periods showed some minor histopathological changes, but there were no differences between treatments. Lesions included oedema and focal haemorrhage in the liver and the spleen was congested. Electron micrographs of tissue samples showed the presence of a few virus particles around the nuclei of kidney, spleen and intestinal cells, and in or around a myelinated nerve within the optic lobes of infected fish during the first 4 days of infection.  相似文献   

15.
Abstract. The aim of this research was lo evaluate the effect of density and feeding level (initially 8 or 16kg/m3 and 1·3% or 2% of present biomass respectively) on productive traits, meat composition and water quality output in farmed rainbow trout, Oncorhynchus mykiss (Walbaum). Individual fish growth shows significant differences in relation to density and feeding level; feed conversion rate is positively Influenced by the lower feeding level and low rearing density. At low density, specific growth rate, protein efficiency ratio and coefficient of variation of individual final weights are more favourable than at high density. Dry matter and lipid composition of meat are positively affected by feeding level; whereas no differences are recorded on the amino acid and fatty acid profiles. Water quality shows differences due to density (NO3 and NH4+) and feeding level (NO2, NH4+ and P).  相似文献   

16.
Abstract. Four cladoceran species of importance as fish food organisms, viz. Daphnia similes (Claus), Simocephalus vetulus (Schodler). Moina macrocopa (Straus) and Ceriodaphnia cornuta (Sars), were raised in the laboratory on nutrient sources including manures, rice bran and Chlorella. The organisms responded better with rice bran and Chlorella.
The productivity of culture organisms appeared to depend upon inoculum and residual densities, indicating a relationship between periodic harvesting and increased production. Associated study reveals the importance of space per individual in the success of laboratory cultures. The optimum space requirement of each species determined by the size of culture organism may vary from 4·5cm3 per individual for small species like Ceriodaphnia to 41·8cm3 per individual for the larger species, Daphnia.  相似文献   

17.
The utilization of oleic acid as an energy source and the effects of oleic acid levels and/or dietary soy bean lecithin (SBL) on oleic acid utilization, growth and survival, and lipid class and fatty acid compositions of freshwater prawn, Macrobrachium rosenbergii (de Man), juveniles were determined.
Increase in levels of dietary oleic acid from 10 to 80 g kg−1 significantly ( P ≤ 0.05) reduced growth, survival and feed conversion efficiency of M. rosenbergii juveniles during the 40-day feeding period. Inclusion of 20 g kg−1 SBL had no significant effect ( P ≥ 0.05) on growth and survival, nor was there any interactive effect between dietary SBL and oleic acid levels.
Body fatty acid profile of prawns reflected dietary fatty acid quantity. The fatty acid composition of prawns fed diets containing 80 g kg−1 oleic acid had tremendously high proportions of oleic acid. Polar lipids, mostly phosphatidylcholine (PC), constituted the bulk of the extracted total lipids. Prawns fed with SBL had significantly ( P ≥ 0.05) higher PC content.
Oleic acid was metabolized for energy by M. rosenbergii juveniles at the same rate regardless of dietary level of SBL and/or oleic acid. Expired 14CO2 accounted for half of the ingested radioactivity 48 h after feeding with labelled diets. No significant difference in the amount of 14CO2 expired by prawns fed the labelled test diets was found. Per cent radioactivity ingested and absorbed into the body was also not significantly different in prawns of the different dietary treatments.  相似文献   

18.
Abstract – Age and growth of juvenile tilapia, Oreochromis niloticus , from Lakes Zwai, Langeno and Chamo (Ethiopia) were studied from microincrements in otoliths. Growth in length was best described by the Gompertz model. Average growth rate of the fish was most rapid in Lake Chamo (0.39 mm  ·  day−1, 1.14%  ·  day−1), intermediate in Lake Zwai (0.20 mm  ·  day−1, 0.72%  ·  day−1) and slowest in Lake Langeno (0.16 mm  ·  day−1, 0.62%  ·  day−1). Similarly, back-calculation from otolith increment widths gave growth rates of 0.28 to 0.43 mm  ·  day−1, 0.15 to 0.32 mm  ·  day−1 and 0.11 to 0.28 mm  ·  day−1 for Chamo, Zwai and Langeno fish, respectively. In addition, Fulton's condition factor was largest for Chamo tilapia and smallest for Langeno tilapia; the difference between fish from Langeno and Zwai was small. Rapid growth of juvenile O. niloticus in Lake Chamo was attributed to warm temperature and better food quality. Note  相似文献   

19.
Abstract. Artificially reproduced pacu, Colossoma mitrei Berg, fingerlings (initial weight 41 g) were stocked (100/m3) in plastic cages (1·0m3) within a freshwater reservoir. Three diets containing 30, 35 and 40% protein were tested in quadruplicate over a 76-day experimental feeding period. Fish were fed 7 days a week, twice daily at a feeding regime of 4% body weight/day from stock to 100g and thereafter at 3% until harvest. Mean water temperature over the trial was 26·7°C. Significant differences were observed in total and daily gain and in protein efficiency ratio, while no differences were obtained in the feed conversion ratio between treatments. Based on this study a 35% protein ration is recommended for pacu within floating cages.  相似文献   

20.
Recirculating raceway systems were examined for their potential as a method for the intensive culture of the marine shrimp Penaeus vannamei Boone. The systems consisted of fiberglass raceways 38 m3 (13.7 m ± 2.4 m ± 1.16 m) and 28 m3 (13.7 m ± 2.4 m ± 0.85 m) each equipped with a vertical screen biofilter, foam fractionators and an ultraviolet ozone generator. All of the systems were enclosed in a commercial greenhouse. Four preliminary growout experiments and two growout experiments with stocking densities of 970 shrimp/m3 and 2,132 shrimp/m3 were completed.
Temperature, pH and salinity remained constant throughout the experiments. Unionized ammonia levels remained below 0.2 mg/L. Nitrite levels ranged from 0.1 to 1.0 mg/L. The 2,132/m2 stocking density resulted in 48% survival, food conversion ratio (FCR) of 1.8 and an average size of 10.8 g. The 970/m3 stocking density resulted in 82% survival, FCR of 2, and an average size of 14 g. Production was 11.4 kg/m3 (114 tons/ha) and 11.0 kg/m3 (110 tons/ha) for the high and low stocking densities, respectively.  相似文献   

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