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1.
We previously established 3 cell lines (PLS10, PLS20 and PLS30) from a chemically-induced prostate carcinoma in F344 rats, and demonstrated high potential for metastasis in nude mice. In the present study, we investigated the feasibility of establishing an orthotopic model using the 3 rat prostate cancer cell lines in immunocompetent rats with the aim of resolving species-mismatch problems and defects of immune systems. The PLS10, PLS20 and PLS30 cell lines were injected into the ventral prostates of 6-week-old rats, which were then sacrificed at experimental weeks 4 and 8. Tumor mass formation was found in rats with PLS10, but not in those with PLS20 or PLS30. Additionally, metastatic carcinomas could be detected in lymph nodes and lungs of PLS10-inoculated rats. Genetic analysis demonstrated K-ras gene mutations in PLS10 and PLS20, but not in PLS30 cells. There were no mutations in p53 and KLF6. In conclusion, we established a syngeneic orthotopic model for prostate cancer in immunocompetent rats simulating human castration-resistant prostate cancer (CRPC), which should prove useful for development and validation of therapeutic agents, especially with immunotherapy.  相似文献   

2.
[目的]通过研究羊胎盘转移因子注射液对健康大白鼠、人工创伤模型大白鼠和健康杂种幼犬的血浆总蛋白(TP)、白蛋白(ALB)、超氧化物歧化酶(SOD)、一氧化氮(NO)和血红蛋白(Hb)含量的影响,探讨供试药品对受试动物的营养作用,进而评估其临床营养功效。[方法]将24只健康大鼠和16只健康杂种幼犬分别随机分为试验组和对照组,为试验组大鼠和试验组幼犬灌服供试药品,并检测给药前后血浆TP、ALB和Hb含量的变化。将45只健康大鼠人工创伤造模后分成低剂量试验组、中剂量试验组和对照组3个组,试验组大鼠使用不同剂量供试药品,于术后3、7、14d测定血浆TP、ALB、SOD和NO含量的变化。[结果]①为健康大鼠灌服供试药品后,与对照组相比,试验组健康大鼠血浆TP、ALB含量显著(P0.05)提高。②为健康杂种幼犬灌服供试药品后3d,与对照组相比,试验组健康杂种幼犬血浆中TP、Hb含量均极显著(P0.01)提高,ALB含量显著(P0.05)提高。③为人工创伤模型大鼠灌服供试药品后,与对照组相比,各试验组术后3d血浆TP、ALB、NO含量均提高;术后7 d中剂量组血浆TP、ALB、NO含量均极显著提高(P0.01),低剂量组显著提高(P0.05);术后14 d,试验组血浆TP、ALB含量恢复到术前,试验组血浆NO含量升高,无显著差异(P0.05)。与对照组相比,术后3 d和7 d,各试验组血浆SOD含量升高,无显著差异(P0.05);术后14d,各试验组SOD含量升高,中剂量组显著(P0.05)升高。[结论]羊胎盘转移因子注射液可显著改善健康大鼠和健康杂种幼犬的营养状况,促进术后大鼠创伤恢复和幼犬的生长发育,提高机体抗病能力,在临床上兼具改善营养和提高免疫力的作用。  相似文献   

3.
利用建立的一种酶联免疫吸附试验 (ELISA)对大鼠胃肠道食糜中的 β 酪啡呔 7(β Casomorphin 7)样物质进行了检测分析。动物试验采用30只成年大鼠 ,随机分为 5组。对照组Ⅰ ,仅喂葡萄糖盐水 ;对照组Ⅱ ,喂大豆和饮水 ;试验组Ⅰ ,仅喂牛奶 ;试验组Ⅱ ,喂不含乳的普通饲料和饮水 ;试验组Ⅲ ,喂混有乳的普通饲料和牛奶。各组均自由采食 ,4d后处死。采集胃和十二指肠食糜 ,对其中 β Casomorphin 7样物质进行定量分析。结果 :在试验组Ⅰ和Ⅲ大鼠的胃、十二指肠食糜中检测到显著量的 β Casomorphin 7样物质 ,平均含量分别为 (1 60 52± 1 0 1 9) ,(1 0 6± 6 85)ng/g和 (2 2 0 48± 1 1 91 ) ,(1 4 2 84±7 72 )ng/g。试验组Ⅱ大鼠的胃和十二指肠食糜中也检测到 β Casomorphin 7样物质 ,提示非乳源的食物蛋白质也可能是酪啡肽的潜在来源  相似文献   

4.
探讨姬松茸水溶性粗多糖对镉中毒小鼠肝脏的保护作用。150只SPF级雄性健康小白鼠随机分为对照组、模型组、试验组1、试验组2、试验组3共5组,模型组与3个试验组采用同一水平镉染毒,但同时对3个试验组给予不同剂量的姬松茸水溶性粗多糖溶液进行保护,试验日程为5周。从第2周开始每周每组取7只小鼠采血,分离血浆,测定并统计ALT、AST、TP生化指标的动态变化。结果表明,与对照组比较,模型组小鼠ALT、AST显著升高(P0.05),TP含量显著降低(P0.05);与模型组比较,试验组2、试验组3小鼠的ALT、AST显著降低(P0.05),TP含量显著升高(P0.05)。这表明镉染毒同时灌胃姬松茸水溶性粗多糖溶液对镉中毒小鼠肝损伤具有保护作用。  相似文献   

5.
Serum and seminal plasma concentrations or activities of acid phosphatase (AP), prostate specific antigen (PSA), and canine prostate specific esterase (CPSE) were measured in normal dogs, dogs with benign prostatic hyperplasia (BPH), dogs with bacterial prostatitis, and dogs with prostatic carcinoma to determine if these assays would be of value in differentiating dogs with prostatic carcinoma from normal dogs, and dogs with other prostatic disorders. In addition, tissue sections of prostatic adenocarcinomas were stained with antiprostatic AP, anti-CPSE, and anti-PSA antibodies to determine if these would be suitable immunohistochemical markers of prostatic carcinoma. Prostate-specific antigen was not detected in canine serum or seminal plasma. Serum and seminal AP activities did not differ significantly between normal dogs and those with prostatic diseases, or among dogs with different prostatic disorders. Serum CPSE activities were significantly higher in dogs with BPH than in normal dogs. Mean serum CPSE activities in dogs with BPH, bacterial prostatitis, and prostatic carcinoma were not significantly different from each other. Slight to moderate immunohistochemical staining of canine prostatic adenocarcinomas was noted for prostatic AP and PSA; most tumors did not stain for CPSE. These results show that proteins of prostatic origin appear in the serum of dogs as a result of prostatic pathology, especially BPH. Canine prostatic adenocarcinoma does not appear to be associated with significant increases in CPSE or AP activities, possibly because of down-regulation of these enzymes by prostatic carcinoma cells. It is also possible that failure to detect significant differences resulted from limited statistical power for some groups and pairwise analyses because of the small number of dogs evaluated.  相似文献   

6.
Diabetes mellitus (DM) and obesity are believed to be risk factors for colorectal cancer in humans. In experiment 1, male nondiabetic Long-Evans Tokushima Otsuka (LETO) rats and Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a model animal of type 2 DM, were whole-body X-irradiated (4 Gy) at 6 and 8 weeks of age and euthanized at 78 weeks of age (n=15, respectively). The incidences of small intestine adenocarcinoma in LETO and OLETF rats were 0% and 30%, respectively. In experiment 2, male LETO and OLETF rats (n=24, respectively) were given s.c. injections of 15 mg/kg azoxymethane (AOM) once weekly for 3 weeks and euthanized at 36 weeks of age. The incidences of Zymbal gland tumors in LETO and OLETF rats were 0% and 67%, respectively (P<0.001), whereas those of small intestine adenocarcinoma were 0% and 43% (P<0.001) and those of cecum/colon adenocarcinoma were 46% and 79% (P<0.05), respectively. Fatty change of hepatocytes was common in OLETF rats (63%) but not in LETO rats. Serum triglyceride and free fatty acid levels in OLETF rats were significantly higher than in LETO rats at sacrifice, whereas serum insulin levels in OLETF rats were very diverse. These data suggest that hyperlipidemia plays a significant role in high susceptibility to lower intestinal tract carcinogenesis in OLETF rats; this strain is susceptible to AOM-induced Zymbal gland carcinogenesis.  相似文献   

7.
Chronic inflammation has been considered an important risk factor for development of prostate cancer. Toll-like receptors (TLRs) recognize microbial moieties or endogenous molecules and play an important role in the triggering and promotion of inflammation. In this study, we examined whether expression of TLR4 and TLR5 was associated with progression of prostate transformation in the transgenic adenocarcinoma of mouse prostate (TRAMP) model. The expression of TLR4 and TLR5 was evaluated by immunohistochemisty in formalin-fixed paraffin-embedded prostate tissue from wild-type (WT) and TRAMP mice. Normal prostate tissue from WT mice showed strong expression of TLR4 and TLR5. However, TLR4 expression in the prostate tissue from TRAMP mice gradually decreased as pathologic grade became more aggressive. TLR5 expression in the prostate tissue from TRAMP mice also decreased in low-grade prostate intraepithelial neoplasia (PIN), high-grade PIN and poorly differentiated adenocarcinoma. Overall, our results suggest that decreased expression of TLR4 and TLR5 may contribute to prostate tumorigenesis.  相似文献   

8.
Malignant neoplasms were present in 39/66 Dicrostonyx groenlandicus of varying ages, examined from a laboratory colony. The presence of multiple neoplasms in some resulted in an overall average of 1.15 tumors/affected animal. Gastric squamous papillomas were present in nine, and locally invasive or metastatic gastric squamous cell carcinomas in a further 36 animals. Three mammary adenocarcinomas, one pancreatic islet cell tumor, one probable pancreatic adenocarcinoma and one adrenal cortical adenoma were also seen in lemmings with gastric tumors. Two others had mammary adenocarcinoma alone, and one animal had bilateral Harderian gland adenocarcinoma. Lesions resembling glomerulonephrosis of rats were seen in 23/51 animals whose kidneys were examined. These findings were not considered artefacts of captivity since concurrent gastric squamous cell carcinoma, mammary adenocarcinoma and glomerulonephrosis were present in the single animal examined directly from the wild at Eskimo Point, Northwest Territories.  相似文献   

9.
Hepatobiliary neuroendocrine carcinoma was diagnosed in 17 cats in a period of 10 years. Seven tumors were of intrahepatic origin, one of which was a composite containing components of epithelial and neuroendocrine carcinoma. Nine tumors were of extrahepatic origin, and one tumor was located in the gall-bladder. The cats were adult and geriatric, and the male : female ratio varied according to tumor group. Hepatomegaly, anorexia, weight loss, and vomiting were the most common clinical signs observed in the cats with hepatic neuroendocrine carcinoma. The cats with extrahepatic neuroendocrine carcinoma showed these signs plus icterus (5/9) and high concentrations of hepatic enzymes. Histologically, the hepatic neuroendocrine carcinomas had two patterns, one with acinar structures separated by vascular stroma lined by cuboidal or columnar cells and the other solid with groups of anaplastic cells separated by vascular stroma. The composite tumor consisted of both bile duct carcinoma and neuroendocrine carcinoma. The extrahepatic neuroendocrine carcinomas and the gallbladder neuroendocrine carcinoma were characterized by solid sheets or groups of round to oval cells with vascular or fibrovascular stroma. Immunohistochemical examination of 10 of the neuroendocrine carcinomas revealed that all 10 stained with neuron-specific enolase; one bile duct carcinoma and the gallbladder carcinoma stained with chromogranin; four of five bile duct carcinomas and the gall bladder carcinoma stained with synaptophysin; and one bile duct carcinoma stained with gastrin. One cat with hepatic carcinoma had duodenal ulcer; in this cat, ultrastructural studies showed neurosecretory granules leading to the diagnosis of Zollinger-Ellison syndrome. In four cats in which necropsy was permitted, carcinomatosis (4/4), lymph nodes (4/4), lungs (2/4), and intestines (1/4) were the metastatic sites. Fourteen of the 17 cats were euthanatized during or immediately after surgery.  相似文献   

10.
探讨和构建Wistar大鼠肺癌的动物模型,以了解肺癌发生发展的过程.采用Wistar大鼠68只,其中试验组60只大鼠(A组30只:三甲基胆蒽(MCA) 5 mg～7 mg,二乙基亚硝胺(DEN) 0.02 mL;B组30只:MCA 7.5 mg～9.5 mg,DEN 0.03 mL),左肺下叶支气管灌注含MCA和DEN的碘油溶液0.1 mL;另8只灌注0.1 mL碘油,作为对照组.试验的3月和6月末分别进行X线检测,发现3月末已有肺部阴影,6月末有50%出现明显的肺部阴影.组织学观察发现1月末已出现肺部原位癌和早期浸润癌;6月末48只发生癌变,致癌率为80%,癌变的Wistar大鼠肺标本中有多个阶段病变共存;共获取支气管黏膜上皮增生44例,不典型增生29例(含鳞状化生7例),原位癌18例,侵袭癌19例,转移癌11例.结果表明,一次性支气管灌注MCA和DEN可引起大鼠肺鳞癌的发生,并获得癌发生过程中各个阶段的标本,该疾病模型为人类肺癌发生机制的研究提供了基础依据.  相似文献   

11.
Adrenal necrosis has been reported as a complication of trilostane application in dogs with hyperadrenocorticism. One suspicion was that necrosis results from the increase of adrenocorticotropic hormone (ACTH) during trilostane therapy. The aim of the current study was to assess the effects of ACTH and trilostane on adrenal glands of rats. For experiment 1, 36 rats were divided into 6 groups. Groups 1.1 to 1.4 received ACTH in different doses (60, 40, 20, and 10 μg/d) infused subcutaneously with osmotic minipumps for 16 wk. Group 1.5 received saline, and group 1.6 received no therapy. For experiment 2, 24 rats were divided into 3 groups. Group 2.1 and 2.2 received 5 and 50 mg/kg trilostane/d orally mixed into chocolate pudding for 16 wk. Eight control rats received pudding alone. At the end of the experiments, adrenal glands were assessed for necrosis by histology and immunohistochemistry; levels of endogenous ACTH and nucleosomes were assessed in the blood. Rats treated with 60 μg ACTH/d showed more hemorrhage and vacuolization and increased numbers of apoptotic cells in the adrenal glands than rats treated with 20 or 10 μg ACTH/d, trilostane, or control rats. Rats treated with 60 μg ACTH/d had a higher amount of nucleosomes in the blood compared with rats treated with 10 μg ACTH/d, trilostane, or saline. We conclude that in healthy rats ACTH, but not trilostane, causes adrenal degeneration in a dose-dependent manner. Results of this study support the hypothesis that adrenal gland lesions seen in trilostane-treated dogs are caused by ACTH and not by trilostane.  相似文献   

12.
将40只SD大鼠随机均分为4组(雌雄各半):对照组、低剂量组、中剂量组和高剂量组.对照组饲喂基础日粮,低剂量组、中剂量组和高剂最组分别饲喂含0.036%、0.144%和0.288%富硒酵母的日粮,持续28 d.在试验过程中,记录大鼠的增重和采食量,计算食物利用率.试验结束时,记录大鼠心脏、脾脏、肝脏、肾脏、睾丸、卵巢重量并计算脏器指数,试验结束时采取血液样本进行血常规指标测定.结果表明,高、中、低剂量组大鼠的食物利用率、心脏、脾脏、睾丸、卵巢指数以及血液常规指标与对照组相比均无显著差异(P>0.05);高剂鼍组大鼠的增重和采食量显著低于对照组(P<0.05),高剂量组大鼠的肾脏指数和肝脏指数显著高于对照组(P<0.05).低、中剂量富硒酵母日粮对大鼠的增重、采食量、肾脏、肝脏指数无明显影响;高剂量富硒酵母日粮对大鼠的增重、采食量、肾脏、肝脏指数有明显影响.  相似文献   

13.
高脂饲粮和高碳水化合物饲粮对大鼠脂肪代谢的影响   总被引：1，自引：0，他引：1  
本试验旨在以相同代谢能水平为基础,研究高脂饲粮和高碳水化合物饲粮对大鼠脂肪代谢的影响。选取48只8周龄雄性SD大鼠,随机分为3组,每组8个重复,每个重复2只。3个组分别饲喂高脂饲粮(HF组)、高碳水化合物饲粮(HC组)和对照饲粮(CON组),试验期9周。结果表明:1)与CON组相比,HF组、HC组大鼠的初重、末重、日增重均无显著差异(P0.05),日采食量极显著降低(P0.01),饲料转化效率极显著升高(P0.01)。2)各组大鼠的Lee’s指数、肾脏指数、胃指数、脾脏指数、肝脏指数无显著差异(P0.05)。3)与CON组和HF组相比,HC组大鼠血清甘油三酯、总胆固醇、高密度脂蛋白胆固醇含量显著或极显著升高(P0.05或P0.01);与HC组和CON组相比,HF组大鼠血清葡萄糖含量极显著增加(P0.01),血清尿素氮含量极显著降低(P0.01)。与CON组相比,HF组和HC组大鼠肝脏甘油三酯含量极显著升高(P0.01),HF组大鼠肝脏总胆固醇含量极显著升高(P0.01)。4)与CON组相比,HF组和HC组大鼠肝脏磷酸烯醇式丙酮酸激酶(PEPCK)mRNA表达量极显著增加(P0.01);与CON组和HC组相比,HF组大鼠肝脏胆固醇调节元件结合蛋白1(SREBP1)mRNA表达量极显著增加(P0.01)。综上所述,相同代谢能水平下,HF组和HC组大鼠体重无显著增加。HC组大鼠血脂升高;HF组大鼠血清葡萄糖、肝脏总胆固醇含量升高,血清尿素氮含量下降。HF组和HC组大鼠肝脏甘油三酯含量升高,且通过肝脏PEPCK和SREBP1基因表达调控途径影响脂肪代谢  相似文献   

14.
本试验旨在研究不同剂量硒蛋白粉对大鼠血常规指标及血液生化指标的影响。选择平均体重为70 g的健康清洁级SD大鼠80只,随机分为4组,每组2个重复,每个重复10只,雌、雄各半。对照组饲喂基础日粮,低、中和高剂量组分别饲喂添加3.5、7和10.5 mg/kg硒蛋白粉的日粮（其含量以硒计）,试验期30 d。结果表明,低、中和高剂量组大鼠血常规指标与对照组相比差异均不显著（P＞0.05）;低、中和高剂量组大鼠血清中血糖、总蛋白含量与对照组相比差异均不显著（P＞0.05）,低剂量组大鼠血清中甘油三酯、低密度脂蛋白含量均显著低于对照组（P＜0.05）;中剂量组大鼠血清中白蛋白/球蛋白及甘油三酯、低密度脂蛋白含量均显著低于对照组（P＜0.05）,而谷丙转氨酶和谷草转氨酶水平均显著高于对照组（P＜0.05）;高剂量组大鼠血清中白蛋白、白蛋白/球蛋白、甘油三酯、低密度脂蛋白含量均显著低于对照组（P<0.05）,而球蛋白、谷丙转氨酶、谷草转氨酶、尿素氮、肌酐、高密度脂蛋白含量均显著升高（P＜0.05）。综上所述,不同剂量硒蛋白粉对大鼠血常规指标无不良影响,低剂量硒蛋白粉除具有降低大鼠血脂作用外对大鼠其他血液生化指标均无不良影响,中、高剂量硒蛋白粉对大鼠的部分血液生化指标有一定影响。  相似文献   

15.
The incidence of mammary adenocarcinoma in Sprague-Dawley female rats, caused by the carcinogen 7,12-dimethylbenz(alpha)anthracene, was influenced by the level of dietary fat fed after exposure to carcinogen. Carcinogen was given by stomach tube to 50-day-old rats, and tumors were evaluated when rats were 9 months old. Rats on diets containing 20% unsaturated fat had a tumor incidence of 97%, while rats changed to a low-fat diet (2% unsaturated fat) three or four weeks after exposure to the carcinogen had an incidence of 45%. Some rats on each diet were given two treatments with the methanol extraction residue of Bacillus Calmette-Guerin, either three and five weeks after carcinogen or four and six weeks after carcinogen. Tumor incidence in the treated group and the untreated group was the same when rats were maintained on the high-fat diet, but tumors in the treated group were larger and the disease was more severe by histological criteria. These tumors were more anaplastic and many were extensively infiltrated with lymphocytes compared to the untreated group. Tumor incidence was significantly lower in rats changed to the low-fat diet (45%) than in those on the high-fat diet (97%), and tumor incidence was reduced to 20% when rats changed to the low-fat diet were treated with methanol extraction residue. The treated group had less severe disease than the untreated group on the low-fat diet. Only half the tumor-bearing rats in this group had malignant tumors, and none were invasive. Methanol extraction residue protected most rats on the low-fat diet against mammary adenocarcinoma, and reduced the severity of disease in those rats that did develop tumors. Methanol extraction residue treatment provided no protection, and even increased the severity of disease, in rats on the high-fat diet.  相似文献   

16.
A study was designed to determine whether phenytoin (PHE) prevents the myocardial necrosis and subsequent fibrosis produced by isoproterenol (ISO). Seven groups of female rats of the Wistar strain were used. Rats in groups 1 and 5 served as controls. Rats in group 3 were injected SC with 85 mg of ISO/kg of body weight for 2 consecutive days. Rats in groups 2 and 6 received 100 mg of PHE/kg orally. Rats in groups 4 and 7 received both PHE and ISO. There were 6 to 9 rats/group. Effects of ISO and PHE were evaluated gravimetrically, histologically, and electrocardiographically. Heart weight/body weight ratios for each group receiving ISO, with or without PHE, were greater than for groups not receiving ISO (P less than 0.05). Light microscopic examination of heart sections of rats given ISO alone or ISO + PHE revealed multiple and diffuse areas of fibrosis. Fibrosis in hearts from rats receiving PHE + ISO was less severe than that in hearts from rats receiving ISO alone, but the difference was not statistically significant. Electrocardiographic changes of statistical significance were not observed in rats receiving any compound (alone or in combination), when compared with the control groups of equal age.  相似文献   

17.
This experiment was carried out to investigate the effects of enriching laboratory cages on agonistic interaction and dominance of rats. In a series of three replicates, 48 rats were housed in groups of four in either ‘standard’ or ‘enriched’ cages for 6 weeks. Successful aggressive and defensive behaviour that ended up in a clear winner and loser were sampled in the first hour of the dark phase of the light/dark cycle every other week. Rats in the ‘complex’ cages showed lower levels of both successful aggressive and successful defensive bouts compared to rats in the ‘standard’ cages. Enriching cages of laboratory rat did not change the social order of the animals in the cage. Thus, enhancing the complexity of cages of laboratory rats by the particular cage modification regimen implemented in this experiment could be considered enrichment and could therefore result in an improvement of welfare in these animals.  相似文献   

18.
为探究死亡受体Fas在镉暴露致大鼠大脑皮质自噬体形成中的作用,将24只21日龄雄性SD大鼠随机分为4组,分别为对照组、镉组、镉与对照病毒共处理组、镉与Fas基因沉默病毒共处理组。试验期间,对照组大鼠自由饮用纯净水,病毒处理组大鼠于第1天以每只1.4×1011vg的剂量通过尾静脉注射相应病毒,4周后镉染毒组大鼠自由饮用镉水(50 mg·L-1),持续90 d。试验结束后,透射电镜观察大脑皮质中自噬体数量,Western blot检测大脑皮质细胞外信号调节激酶1/2(Erk1/2)、p-Erk1/2、自噬相关蛋白7(ATG7)、自噬相关基因(Beclin-1)、微管相关蛋白1轻链3(LC3)蛋白表达水平,免疫荧光染色检测LC3表达水平。结果显示,镉暴露增加大脑皮质中自噬体数量,极显著激活Erk1/2并上调ATG7、Beclin-1、LC3蛋白表达水平(P<0.01);Fas基因沉默抑制镉引起的自噬体数量增加,极显著抑制镉致Erk1/2激活及ATG7、Beclin-1、LC3蛋白表达水平升高(P<0.01)。结果表明,Fas通过激活Erk1/2参与镉致大鼠大脑皮质自噬体形成。  相似文献   

19.
本研究旨在探讨香菇多糖对大肠杆菌攻毒大鼠空肠形态结构、上皮细胞数量和紧密连接蛋白表达的影响。将24只SD大鼠随机分成4个组(A、B、C、D组),每组6个重复,每个重复1只。A、B组饮用蒸馏水,C、D组饮用蒸馏水中添加20μg/m L的香菇多糖;试验第15天B、D组灌服2 m L浓度为1×1010CFU/m L大肠杆菌K88,A、C组灌服等量生理盐水。试验第18天心脏采血处死,取空肠固定和冻存,制作石蜡切片,苏木精-伊红(HE)染色和高碘酸雪夫氏(PAS)染色,测定绒毛高度、隐窝深度、绒毛宽度、上皮内淋巴细胞(IEL)数量和上皮杯状细胞(GC)数量,计算绒毛高度与隐窝深度的比值(V/C);并采用蛋白质印迹(Western-blot)法测定空肠紧密连接蛋白Occludin的表达水平。结果表明:各组绒毛高度和绒毛宽度无显著差异(P0.05)。C组隐窝深度极显著低于A、B组(P0.01),显著低于D组(P0.05)。C组V/C极显著高于A、B、D组(P0.01)。C组IEL数量极显著低于B、D组(P0.01),显著低于A组(P0.05)。C组上皮GC数量极显著高于A、D组(P0.01),与B组差异不显著(P0.05)。D组Occludin表达水平明显高于A、B和C组,B组Occludin表达水平高于A组。综上,大鼠饮用水中添加香菇多糖可改善大鼠空肠形态结构,并提高其抵抗大肠杆菌感染能力,促进Occludin的表达。  相似文献   

20.
Canine bile duct carcinoma   总被引：2，自引：0，他引：2  
Twenty-four adenocarcinomas of the hepatobiliary system were found among 110 primary hepatic neoplasms: 22 of these were intrahepatic, one involved the extrahepatic bile duct and one the gall bladder. Histologically, 10 intrahepatic neoplasms were classified as cholangiocarcinoma, and 12 as bile duct cystadenocarcinoma. The former were characterized by tubular structures lined by anaplastic cuboidal or columnar cells with diffuse fibrous stroma, and the latter by multiple cystic structures with papillary and solid areas. Half the bile duct cystadenocarcinomas showed benign cysts and transition to adenocarcinomas. This was seen in only one case of cholangiocarcinoma. The extrahepatic bile duct carcinoma was characterized by tubular structures lined with flattened cuboidal cells with scant stroma. The gall bladder adenocarcinoma contained many acinar structures lined with columnar or cuboidal cells and separated by thin stroma. Distant metastasis was found in 87.5% (21 of 24) sites of metastasis.  相似文献   

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