共查询到16条相似文献，搜索用时 151 毫秒
1.
为探究松辽黑猪视黄醇结合蛋白4（RBP4）基因多态性及其与繁殖性状的关联性,试验选取132头松辽黑猪作为研究对象,以与猪繁殖性状相关的RBP4基因为候选基因,采用PCR-HRM分析与PCR产物直接测序相结合的方法检测松辽黑猪RBP4基因部分外显子的单核苷酸多态性（SNP）,分析RBP4基因SNP与母猪总产仔数、产活仔数、乳头数及仔猪初生重、3周龄重、断奶重的关联性。结果显示,松辽黑猪RBP4基因第3、5外显子上各存在1个SNP位点,分别命名为为G45A和C170T,其中在G45A位点检测到GG、GA和AA 3种基因型,在C170T位点检测到CC和CT 2种基因型。群体遗传参数分析及卡方适合性检验结果显示,RBP4基因G45A位点在松辽黑猪群体中分布较为均匀,遗传变异程度处于中等水平,偏离Hardy-Weinberg平衡（P<0.01）;C170T位点在松辽黑猪群体中分布不均匀,遗传变异程度较低,未偏离Hardy-Weinberg平衡（P>0.05）。关联分析结果表明,在RBP4基因G45A位点上,3种基因型的总产仔数及产活仔数均表现为：GG > GA > AA,其中GG基因型的总产仔数、产活仔数显著高于AA基因型（P<0.05）;GG基因型仔猪3周龄重显著低于GA基因型（P<0.05）;GG基因型仔猪断奶重显著低于GA及AA基因型（P<0.05）;各基因型在仔猪初生重及母猪乳头数上未表现出显著差异（P>0.05）。在C170T位点上,不同基因型松辽黑猪的总产仔数、产活仔数、乳头数及仔猪初生重、3周龄重、断奶重均无显著差异（P>0.05）。综上,RBP4基因G45A位点对松辽黑猪产仔数具有显著影响,但该位点是否可作为松辽黑猪产仔数相关的遗传标记,有待进一步研究;RBP4基因C170T位点可能不是松辽黑猪繁殖性状相关的突变位点,在后期工作中需扩大群体做进一步验证。 相似文献
2.
为探究松辽黑猪METTL23基因多态性及其与繁殖性状的关联性,试验选取178头松辽黑猪母猪为研究对象,利用Sanger直接测序法查找METTL23基因外显子1~5的单核苷酸多态性(SNP),使用SPSS 19.0软件分析METTL23基因SNP位点与松辽黑猪繁殖性状(总产仔数、产活仔数、断奶仔猪数、初生重、3周龄重、断奶重、乳头数)的关联性。结果显示,仅在松辽黑猪METTL23基因外显子4发现1个SNP位点,命名为A62G,在A62G位点上检测到3种基因型,分别是AA、AG和GG。GG和G分别为优势基因型和优势等位基因,且A62G位点处于Hardy-Weinberg平衡状态(P>0.05)。该位点的遗传纯合度(Ho)为0.6516,高于遗传杂合度(He,0.3484),说明其在松辽黑猪群体中的变异较小;多态信息含量(PIC)为0.2877,属于中度多态位点(0.25<PIC<0.5),说明该遗传标记能够提供一定量的遗传信息。关联分析结果表明,GG基因型个体总产仔数、产活仔数和断奶仔猪数均显著或极显著高于AA基因型个体(P<0.05;P<0.01),总产仔数还显著高于AG基因型个体(P<0.05);AG基因型个体断奶仔猪数显著高于AA基因型个体(P<0.05);初生重、3周龄重、断奶重、乳头数各基因型间差异均不显著(P>0.05)。综上所述,METTL23基因外显子4存在多态性位点A62G,该位点与松辽黑猪产仔数存在显著关联,可作为松辽黑猪产仔数的遗传标记,用于分子育种。 相似文献
3.
胎次、产仔月份及纯繁/杂交对母猪产仔性能的影响 总被引：1，自引：0，他引：1
本研究统计分析了部分非遗传因素(胎次、产仔月份及纯繁/杂交)对长白、大白和杜洛克母猪产仔性能(总产仔数、产活仔数及初生窝重)的影响,旨在为后备母猪的选种提供指导。结果表明,2~5胎仔猪的初生窝重较重;其中2、3胎的初生窝重极显著高于头胎及6胎之后的仔猪初生窝重(P<0.01)。3月份总产仔数、产活仔数及初生窝重极显著高于其他月份(P<0.01),且11月份初生窝重显著低于其他月份(P<0.05)。长白纯繁母猪在产活仔数方面极显著优于大白纯繁母猪和长白×大白杂交母猪(P<0.01);在初生窝重方面,大白×长白杂交母猪的初生窝重极显著高于大白纯繁母猪和长白×大白杂交母猪(P<0.01)。第2胎与3~4胎、第3胎与第4胎的总产仔数呈极显著正相关(P<0.01)。综上所述,该猪场母猪3~6胎的产仔性能较好,且以1~4月份的产仔性能为佳;配种方式以长白纯繁和大白×长白杂交为宜;第2胎产仔性能可作为后备母猪选留的重要依据。 相似文献
4.
为探究寡腺苷酸合成酶1（oligoadenylate synthase 1,OAS1）基因多态性与松辽黑猪繁殖性状的关联性,试验选取130头松辽黑猪母猪为研究对象,利用Sanger直接测序法测序查找OAS1基因外显子1~8的SNP位点,使用SPSS 19.0软件分析OAS1基因SNP位点与松辽黑猪繁殖性状的关联性。结果显示,在松辽黑猪OAS1基因外显子2、3和6上共检测到33个突变位点;其中在外显子2的110 bp处存在1个SNP位点（G110C）,存在3种基因型：GG、GC和CC;在外显子3的176 bp处存在1个SNP位点（C176T）,存在3种基因型：CC、CT和TT;在外显子6的145 bp处存在1个SNP位点（C145T）,存在3种基因型：CC、CA和AA;在166 bp处存在1个SNP位点（G166A）,存在3种基因型：GG、GA和AA;在206 bp处存在1个SNP位点（A206G）,存在3种基因型：AA、AG和GG。卡方适合性检验结果显示,松辽黑猪OAS1基因G110C突变位点符合Hardy-Weinberg平衡状态,C176T、C145A、G166A和G206A位点均偏离Hardy-Weinberg平衡状态。群体遗传参数分析结果显示,各SNPs位点遗传杂合度均位于中等水平,为中度多态（0.25<PIC<0.5）。关联分析结果发现,G110C位点GC基因型个体总产仔数、产活仔数和断奶仔猪数均显著高于GG基因型个体（P<0.05）;C176T位点CT基因型个体断奶仔猪数显著高于CC基因型个体（P<0.05）;C145T位点CC基因型个体总产仔数和产活仔数均显著高于AA基因型个体（P<0.05）;G166A位点GA基因型个体断奶仔猪数显著高于GG基因型个体（P<0.05）;A206G位点GG基因型个体总产仔数和产活仔数显著高于AA基因型个体（P<0.05）。结果表明,OAS1基因外显子区存在突变位点,对松辽黑猪部分繁殖性状有显著性影响。 相似文献
5.
试验旨在探究松辽黑猪NR5A2基因多态性及其与繁殖性状的关联性。选取130头松辽黑猪作为研究对象,利用PCR直接测序法查找NR5A2基因6个外显子的SNP位点,通过HRM分型技术分析SNP位点的多态性,使用SPSS 19.0软件分析NR5A2基因SNP位点多态性与母猪总产仔数、产活仔数、仔猪初生重、3周龄重、断奶重及断奶仔猪数、乳头数的关联性。结果显示,松辽黑猪NR5A2基因第6外显子上存在1个SNP位点（C99T）,检测到3种基因型：CC、CT和TT。群体遗传参数分析及卡方适合性检验结果显示,该SNP位点在松辽黑猪群体中处于Hardy-Weinberg平衡状态（P>0.05）,但C/T突变位点杂合度相对较低,在松辽黑猪群体中的变异较小,属于中度多态（0.25<PIC<0.5）。关联性分析结果表明,在NR5A2基因C99T位点上,CC基因型个体的总产仔数、产活仔数及断奶仔猪数均最高,且显著高于TT基因型（P<0.05）,产活仔数及断奶仔猪数均显著高于CT基因型（P<0.05）;各基因型仔猪初生重、3周龄重、断奶重及乳头数均无显著差异（P>0.05）。综上所述,NR5A2基因第6外显子存在突变位点C99T,与松辽黑猪产仔数存在显著关联性,但该突变位点能否作为松辽黑猪产仔数的遗传标记,需要对更大的群体进行进一步研究。 相似文献
6.
【目的】 分析胎次、配种季节和与配公猪3个固定效应对大白母猪妊娠天数的影响,估计妊娠天数的遗传参数并探索其与总产仔数、产活仔数、初生窝重、死胎数、木乃伊数的关系。【方法】 收集2 138头大白母猪妊娠天数、总产仔数、产活仔数、初生窝重、死胎数及木乃伊数6种生产表型,共包含4个胎次（1~4）的4 261条记录。利用一般线性模型分析试验猪群的固定效应对妊娠天数的影响,并采用多性状重复力模型估计妊娠天数与其他繁殖性状的遗传参数（遗传力、重复力、遗传相关、表型相关）。【结果】 大白母猪平均妊娠天数为116.08 d,经t检验分析与114 d差异显著（P<0.05）。胎次对妊娠天数有显著影响,且第1胎妊娠期显著短于第2胎（P<0.05）;配种季节对妊娠天数有极显著影响（P<0.01）,春季配种的母猪妊娠天数最短,秋季配种的母猪妊娠天数最长,相差0.73 d （P<0.05）;与不同公猪个体配种后母猪的妊娠天数之间有显著差异（P<0.05）,差异最大达3.05 d。妊娠天数遗传力为0.1603,与总产仔数、产活仔数、初生窝重、死胎数及木乃伊数之间均呈现负表型和负遗传相关。【结论】 妊娠天数受到多种因素的影响,且具有遗传性,与母猪总产仔数等繁殖性状有显著相关,可作为养猪生产的间接指标,对准确把握母猪预产期、实现精准饲养管理具有重要意义。 相似文献
7.
《中国畜牧兽医》2020,(8)
为探究松辽黑猪视黄醇结合蛋白4(RBP4)基因多态性及其与繁殖性状的关联性,试验选取132头松辽黑猪作为研究对象,以与猪繁殖性状相关的RBP4基因为候选基因,采用PCR-HRM分析与PCR产物直接测序相结合的方法检测松辽黑猪RBP4基因部分外显子的单核苷酸多态性(SNP),分析RBP4基因SNP与母猪总产仔数、产活仔数、乳头数及仔猪初生重、3周龄重、断奶重的关联性。结果显示,松辽黑猪RBP4基因第3、5外显子上各存在1个SNP位点,分别命名为为G45A和C170T,其中在G45A位点检测到GG、GA和AA 3种基因型,在C170T位点检测到CC和CT 2种基因型。群体遗传参数分析及卡方适合性检验结果显示,RBP4基因G45A位点在松辽黑猪群体中分布较为均匀,遗传变异程度处于中等水平,偏离Hardy-Weinberg平衡(P0.01);C170T位点在松辽黑猪群体中分布不均匀,遗传变异程度较低,未偏离Hardy-Weinberg平衡(P0.05)。关联分析结果表明,在RBP4基因G45A位点上,3种基因型的总产仔数及产活仔数均表现为:GGGAAA,其中GG基因型的总产仔数、产活仔数显著高于AA基因型(P0.05);GG基因型仔猪3周龄重显著低于GA基因型(P0.05);GG基因型仔猪断奶重显著低于GA及AA基因型(P0.05);各基因型在仔猪初生重及母猪乳头数上未表现出显著差异(P0.05)。在C170T位点上,不同基因型松辽黑猪的总产仔数、产活仔数、乳头数及仔猪初生重、3周龄重、断奶重均无显著差异(P0.05)。综上,RBP4基因G45A位点对松辽黑猪产仔数具有显著影响,但该位点是否可作为松辽黑猪产仔数相关的遗传标记,有待进一步研究;RBP4基因C170T位点可能不是松辽黑猪繁殖性状相关的突变位点,在后期工作中需扩大群体做进一步验证。 相似文献
8.
实验旨在挖掘大白猪磷酸甘油酸变位酶2(PGAM2)基因单核苷酸多态位点（SNPs），分析遗传多样性、连锁不平衡程度及其不同基因型与生长、繁殖性状的相关性。选取大白母猪316头，提取DNA，采用混合DNA样本PCR扩增产物测序，确定PGAM2基因SNPs位点。采用多重PCR靶向基因捕获技术对每个个体进行目标SNPs位点分型，并分别与大白猪初产和经产的总产仔数、产活仔数、木乃伊数和死胎数进行关联分析，与大白猪生长性状的达100 kg日龄、达100 kg背膘厚、达100 kg眼肌厚和初生重进行关联分析。共鉴定出5个突变位点，均为已知SNP，其中rs331014516和rs346188790位点为稀有突变，不适合用作分子标记；所有位点均处于Hardy-Weinberg平衡（P>0.05）;rs331014516与rs346188790完全连锁，rs80977507与rs80902904完全连锁且与rs343549757强连锁；rs80977507及其完全连锁位点与初产母猪总产仔数、产活仔数显著相关；rs343549757位点与大白猪经产死胎数显著相关。这5个位点的基因型与大白猪达100 ... 相似文献
9.
《浙江畜牧兽医》2015,(4)
试验选用浙江某种猪场2010-2013年出生的加系大约克种猪共4382窝的繁殖性能数据,统计分析不同胎次和配种季节对总产仔数、产活仔数、总死产数、畸形胎数、木乃伊胎数、死胎数和初生窝重的影响。结果表明:胎次对种猪产仔性能有极显著影响(P0.01),母猪1~6胎总产仔数、产活仔数、初生窝重随胎次增加而增加,第6胎达到最高峰,且显著(P0.05)或极显著(P0.01)高于其他胎次;总死产数及死胎数则7~8胎极显著高于其他胎次(P0.01);但胎次对产畸形胎和木乃伊胎数影响不显著(P0.05)。配种季节对种猪产仔性能影响极显著(P0.01),冬季配种的总产仔数,产活仔数,初生窝重显著高于其他配种季节(P0.05);总死产数及死胎数极显著低于其他配种季节(P0.01);但冬季配种产木乃伊胎数极显著高于夏、秋两季(P0.01),显著高于春季(P0.05)。研究表明,加系大约克种猪的最佳产仔胎次为3~6胎,冬季配种的产仔性能明显高于其他配种季节。 相似文献
10.
《中国畜牧杂志》2016,(5)
本研究旨在检测嘉兴黑猪ESR2、FSHβ和PRLR基因的多态性及其对繁殖性能的影响。通过聚合酶链式反应-单链构象多态性分析(PCR-SSCP)检测嘉兴黑猪273个个体8个单核苷酸多态性(SNP)位点的多态性,并与母猪繁殖性能(总产仔数、产活仔数、死胎数、两胎间隔和初生均重)开展关联分析。结果表明:ESR2基因外显子1的A221G与母猪的总产仔数、产活仔数、死胎数和初生均重显著相关(P0.001),PRLR基因的A13901T影响总产仔数、产活仔数、死胎数和初生均重(P0.001);上述2个位点合并基因型分析表明基因型为AA-TT和AGTT的个体繁殖性状显著高于其他部分基因型个体(P0.001);其余位点并未发现与繁殖性状显著关联(P0.05)。本研究与常用的ESR2、FSHβ和PRLR基因酶切位点分析不同,筛选并确定了8个嘉兴黑猪新的SNP位点,其中2个位点和繁殖性状紧密相关,这为提高嘉兴黑猪种群的遗传育种水平和生产繁殖能力提供了理论支撑。 相似文献
11.
This study was aimed to find the molecular markers significantly associated with litter size traits and uniformity of the piglet's birth weight,and provided a basis for genetic improvement of reproductive traits in pigs.This study was based on the genome-wide selective sweep analysis of pig conducted by the research group in the previous studies,two SNPs (rs338309012 and rs335824784) on porcine chromosome 3 were selected,and the genotyping-in-thousand by sequencing was used,the relationship between two SNPs and litter size traits (including total number born,total number born alive,number of stillborn pigs and number of mummified pigs,etc.) and the uniformity of the piglet's birth weight was association analyzed in Danish Yorkshire and American Yorkshire pigs.The results showed that rs338309012 were significantly associated with the total number born and the number of mummified pigs (P<0.05),and the total number born and the number of mummified pigs of AC genotype sows was significantly higher than CC genotype (P<0.05).rs335824784 was significantly associated with the effective number of born alive and the number of mummified pigs (P<0.05),and was also extremely significantly associated with the total number born alive and the number of stillborn pigs (P<0.01).The effect of different genotypes on the total number born alive and effective number of born alive were GG > CG > CC.On the contrary,the effect of different genotypes on the coefficient of variation of the birth weight of piglets,the number of dead and mummified fetuses were GG < CG < CC.Thus,the GG genotype sows with rs335824784 not only had more total number born alive,but also had regular piglet's birth weight and lower number of stillborn and mummified pigs,which could be used as a molecular marker to improve the litter size traits and uniformity of the piglet's birth weight. 相似文献
12.
Our objective was to estimate responses in reproductive traits in the Nebraska Index line (I) after 19 generations of selection for increased litter size. Responses were estimated in dams producing pure line, F1, and three-way cross litters. A total of 850 litters were produced over six year-seasons, including 224 pure line litters, 393 F1 litters produced from I and C females mated with Danbred NA Landrace (L) or Duroc-Hampshire (T) boars, and 233 litters by F1 L x I and L x C females mated with T boars. Contrasts of means were used to estimate the genetic difference between I and C and interactions of line differences with mating type. Farrowing rates of lines I (u = 91.0%) and C (u = 92.8%) did not differ. Averaged across all genetic groups, mean number born alive per litter was 10.1 pigs, and number and weight of pigs weaned per litter, both adjusted for number nursed and weaning age of 12 d, were 9.7 pigs and 34.4 kg, respectively. Averaged across mating types, direct genetic effects of I were greater than C (P < 0.05) for total born (3.53 pigs), number born alive (2.53 pigs), number of mummified pigs (0.22 pig), and litter birth weight (2.14 kg). The direct genetic effect of line I was less than C (P < 0.05) for litter weaning weight (-1.88 kg). Interactions of line effects with crossing system were significant (P < 0.05) for total number born, number of stillborn pigs, number weaned, and litter weaning weight. In pure line litters, I exceeded C by 4.18 total pigs and 1.76 stillborn pigs per litter, whereas the estimate of I-C in F1 litters was 2.74 total pigs and 0.78 stillborn pig per litter. The contrast between I and C for number weaned and litter weaning weight in pure litters was 0.32 pig and -0.28 kg, respectively, compared with 0.25 pig and -2.14 kg in F1 litters. Crossbreeding is an effective way to use the enhanced reproductive efficiency of the Index line. 相似文献
13.
Comparison of the multi‐trait animal model and the traditional repeatability model was carried out using data obtained from 6,424 Landrace and 20,835 Yorkshire sows farrowed from January 2000 to April 2018 in order to estimate genetic parameters for litter traits at different parities. Specifically, records of the total number born (TNB), number born alive (NBA), total number of mortality (MORT), number of stillborn (NSB) and number of mummified pigs (MUM) were used. Although results showed the heterogeneity of heritability for litter traits at different parities, the mean heritability estimates from the multi‐trait model were found to be higher than those of the repeatability model for all traits in both pig breeds. In terms of genetic correlation between parities, a slight difference in genetic control in the first parity was noted for TNB and NBA in Landrace and Yorkshire pigs. The correlation between the first parity and later parities ranged from 0.48 to 0.74 for TNB and NBA in both breeds. Moreover, genetic correlation between parities for MORT and NSB was observed to be high for parities higher than 2 in Yorkshire pigs. For MUM, genetic correlation between the first and other parities was generally low in both breeds, indicating that culling pigs on the basis of MUM at the first parity could probably be unreasonable. Overall, the results of this study suggest that the multi‐trait approach for litter size traits is useful for the accurate estimation of genetic parameters. 相似文献
14.
苏太猪SLA-DQA基因SNP检测及其对部分经济性状的遗传效应分析 总被引：1，自引：0，他引：1
实验对苏太猪SLA-DQA基因的第4外显子进行多态性分析,并初步探讨DQA基因作为影响苏太猪经济性状候选基因的可能性。PCR-SSCP分析共检测到CC、CD和DD 3种基因型;在第4外显子检测到G5233A突变,导致了氨基酸Thr到Ala的改变。结果表明:苏太猪3种基因型个体的初生重、35日龄断奶体重、4月龄体重、6月龄体重以及60 kg体尺和背膘厚等生长发育指标差异不显著(P0.05);DD基因型第3胎次总产仔数、产活仔数、初生窝重均高于CC和CD基因型的个体,尤其是断奶仔猪数和断奶窝重显著高于CC和CD基因型的个体(P0.05),但CC和CD基因型个体间繁殖性能差异不显著(P0.05)。 相似文献
15.
Examination of the relationship between the estrogen receptor gene and reproductive traits in swine 总被引：13，自引：0，他引：13
The relationship between estrogen receptor (ESR) genotype and reproductive traits in a population of Yorkshire, Large White, and crossbred animals was studied. Reproductive tract and litter data were analyzed for associations with ESR genotype, parity, and breed. Forty-six Yorkshire, 31 Large White, and 70 crossbred females from the above population were mated to Hampshire boars and slaughtered at 75 d of gestation. Data collected included ovulation rate, uterine horn length, number of fetuses, fetal weight, uterine weight, number of mummies, fetal sex, fetal placement, fetal survival, and fetal space. Data were analyzed using a model that included the fixed effects of ESR genotype, breed, parity, and all significant two-way interactions. Litter data representing 212 litter records were analyzed in a model that included the fixed effects of ESR genotype of dam, parity, farrowing month, dam breed, sire breed, and all significant two-way interactions. The ESR genotype was significantly associated with the total litter weight of piglets born and total litter weight of piglets born alive. Dams with the AA genotype had significantly (P = 0.04) heavier litters at birth (14.44 +/- 0.36 kg) than dams with the BB genotype (13.43 +/- 0.47 kg). Ovulation rate was significantly (P < 0.05) different between animals of parity 1 (17.22 +/- 0.41) and parity > or = 3 (19.92 +/- 0.85). Significant breed effects were observed for fetal weight, with purebred Large White animals having a greater fetal weight per horn (3,909 +/- 114 g) than purebred Yorkshire animals (3,553 +/- 92 g). Notable, but nonsignificant, trends with respect to ESR genotype were also observed for number of piglets alive at weaning and total litter weight at weaning. The ESR gene is positively associated with several previously uninvestigated reproductive traits. 相似文献
16.
本研究旨在分析影响大白猪繁殖性能因素(批次管理体系和精液类型)与遗传参数估计,为目标群体的遗传评估及生产管理奠定理论基础。利用DMU和ASREML软件分析2 026头大白母猪共4 512窝繁殖记录,涉及4个繁殖性状(总产仔数、产活仔数、21日龄窝重和断奶仔猪数)。结果表明:大白猪4个繁殖性状的遗传力估计值均在0.10左右,各性状间均成强正遗传相关(0.54以上)。精液类型(鲜精和冻精)效应对断奶仔猪数和21日龄窝重影响不显著(P>0.05),对总产仔数和产活仔数影响显著(P<0.05),其中冻精在总产仔数和产活仔数上较鲜精均少1头左右(P<0.05)。批次管理体系研究表明,由单周批次管理体系转变为4周批次管理体系后,母猪繁殖性能有所下降,产活仔数、断奶仔数猪和21日龄窝重分别下降0.51头、0.61头和10.13 kg。 相似文献