共查询到20条相似文献，搜索用时 62 毫秒
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旨在制备毒害艾美耳球虫(Eimeria necatrix)配子体抗原EnGAM22单克隆抗体,用Ni-NTA亲和层析纯化的重组抗原rEnGAM22免疫BALB/c小鼠,免疫3次后,取脾细胞与骨髓瘤细胞SP2/0进行融合,用预先建立的ELISA方法筛选阳性杂交瘤细胞,经有限稀释法对阳性杂交瘤细胞进行4次亚克隆后,获得2株能稳定分泌特异性抗体的杂交瘤细胞株并命名为2F3和3D3;对单抗亚类和特异性进行鉴定,应用单抗识别天然蛋白和虫体定位。结果显示,单克隆抗体2F3和3D3亚类分别为IgG2a、IgG2b,纯化后腹水效价分别为1:256 000、1:64 000,能特异性识别重组抗原rEnGAM22和毒害艾美耳球虫天然配子体蛋白;免疫荧光定位显示单克隆抗体2F3和3D3能特异性识别大配子体的成壁体和卵囊壁,表明EnGAM22抗原参与卵囊壁的形成。制备的单克隆抗体为研究球虫卵囊壁形成的分子机制奠定了基础。 相似文献
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E. Tydén M. Löfgren M. Hakhverdyan H. Tjälve P. Larsson 《Journal of veterinary pharmacology and therapeutics》2013,36(4):370-375
In the present study, we examined the gene expression of cytochrome P450 3A (CYP3A) isoenzymes in the tracheal and bronchial mucosa and in the lung of equines using TaqMan probes. The results show that all seven CYP3A isoforms identified in the equine genome, that is, CYP3A89, CYP3A93, CYP3A94, CYP3A95, CYP3A96, CYP3A97 and CYP3A129, are expressed in the airways of the investigated horses. Though in previous studies, CYP3A129 was found to be absent in equine intestinal mucosa and liver, this CYP3A isoform is expressed in the airways of horses. The gene expression of the CYP3A isoenzymes varied considerably between the individual horses studied. However, in most of the horses CYP3A89, CYP3A93, CYP3A96, CYP3A97 and CYP3A129 were expressed to a high extent, while CYP3A94 and CYP3A95 were expressed to a low extent in the different parts of the airways. The CYP3A isoenzymes present in the airways may play a role in the metabolic degradation of inhaled xenobiotics. In some instances, the metabolism may, however, result in bioactivation of the xenobiotics and subsequent tissue injury. 相似文献
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C. S. Bailey R. L. Kitchell M.J. Guinan J.W. Sharp 《Anatomia, histologia, embryologia》1992,21(1):23-31
The dorsal root origins of cutaneous nerves supplying the feline pelvic limb were determined electrophysiologically in 11 cats. Cutaneous nerves were surgically exposed and the presence or absence of an evoked potential in response to stimulation of individual dorsal roots was noted. The dorsal cutaneous branches of L3-L5 and S3, and the lateral cutaneous branch of L3 each arose solely from their parent spinal nerves. The L7, S1, and S2 dorsal cutaneous branches had multiple dorsal root origins. The lateral cutaneous femoral nerve originated from L3-L6 dorsal roots in 4 patterns of origin, and the saphenous nerve originated from L4-L6 dorsal roots in 2 patterns of origin. The lateral and caudal cutaneous sural nerves originated from L6-S1 roots in 2 and 3 patterns, respectively. The lateral and medial plantar nerves arose from L6-S2 roots in 4 and 2 patterns, respectively. The superficial and deep peroneal nerves originated from L6-S1 roots in 2 and 3 patterns, respectively. The caudal cutaneous femoral nerve or its branches arose from L7-S3 in 8 origin patterns. The dorsal nerve of the penis and the superficial perineal nerve arose from L7-S3 and S1-S3 roots, respectively, each in 4 patterns. A subtle correlation between plexus type and dorsal root origins of the cutaneous nerves was noted. 相似文献
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为了探究毛壳素对绒山羊脂肪间充质干细胞(gADSCs)组蛋白甲基化修饰差异的影响。本研究采用不同浓度的毛壳素对gADSCs进行不同时间的处理，以期筛选出对细胞活性影响最低且能够最大程度抑制gADSCs组蛋白甲基化的药物处理浓度及时间。以最适药物浓度和时间处理组为试验组，无药物处理组为对照组，通过实时荧光定量PCR检测H3K9 me2和H3K9 me3甲基化相关酶和胚胎发育多潜能基因mRNA表达水平的改变，并检测毛壳素对组蛋白H3K9 me2和H3K9 me3蛋白表达水平的影响。结果显示，20 nmol·L-1的毛壳素处理gADSCs 48 h时对细胞活性影响较小，组蛋白甲基化转移酶G9A的表达显著降低(P<0.05)，为最适处理浓度和时间。实时PCR结果显示，试验组H3K9 me2和H3K9 me3甲基化转移酶EHMT1、EHMT2、SUV39H1、SUV39H2表达显著降低(P<0.05)，H3K9 me2和H3K9 me3去甲基化酶KDM3A、KDM3B、KDM4B、KDM4D表达显著增高(P<0.05)，多潜能性相关基因SOX2、OCT4、NANOG表达量增高。免疫荧光和WB结果显示，处理组H3K9 me2蛋白水平无明显变化，而H3K9 me3蛋白水平显著降低(P<0.05)。20 nmol·L-1的毛壳素处理gADSCs 48 h后可以显著降低组蛋白H3K9 me2和H3K9 me3的甲基化修饰作用，提高多潜能性相关基因的表达。 相似文献
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甲状腺激素对乌骨鸡蛋白质沉积影响的研究 总被引：1，自引：0，他引：1
选用640只雪峰鸟骨鸡，分别饲喂添加不同水平（0．1，0．3，0．5mg／kg）的甲状腺激素（T3）；5～8周龄分成2个组，一组停止添加T，，另一组继续添加T3，以研究甲状腺激素（T3）对雪峰乌鸡蛋白质沉积是否具有后续作用。结果表明：（1）甲状腺激素在2周龄以前可显著（P〈0．01）提高鸟骨鸡的蛋白质沉积；适合量为0．1mg／kg；（2）在乌骨鸡后期日粮中继续添加T3，则乌骨鸡的生长和蛋白质沉积被抑制；后期日粮中停止添加T3后，T3对乌骨鸡蛋白质沉积的抑制作用被解除，生长得到恢复。 相似文献
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旨在克隆猪NR1H3基因的可变剪接体,并预测编码蛋白的结构与功能,研究其转录本的表达特性。本试验以马身猪为研究对象,采用RT-PCR及克隆测序技术对猪NR1H3基因CDS区进行扩增和克隆,采用生物信息学方法分析NR1H3蛋白的生物学特性,采用qRT-PCR技术检测NR1H3基因在心、肝、脾、肺、肾、胃、小肠、股二头肌、腰大肌和皮下脂肪组织中的表达谱及在脂肪组织中的发育性表达规律。结果表明,本研究成功克隆出猪NR1H3基因的两个转录本NR1H3-1和NR1H3-2,其中NR1H3-2为新发现的转录本（MN082630）,其CDS区全长1 203 bp,编码的蛋白质含400个氨基酸,属于中性不稳定蛋白;与NR1H3-1相比,NR1H3-2缺失了长度为95 bp的第9外显子,编码的蛋白质NR1H3-2比NR1H3-1少了一个肝X受体的配体结合结构域;氨基酸的相似性分析发现,猪NR1H3蛋白氨基酸序列与北大西洋小须鲸、山羊、绵羊、抹香鲸、长江江豚等物种的相似性较高。NR1H3-1和NR1H3-2在所检测组织中均有表达,且在不同组织间的表达量具有显著差异（P<0.05）;NR1H3-1转录本在小肠中表达量最高,其次是脾、肝、股二头肌等,在心脏中表达量最低;NR1H3-2则在肝中表达量最高,其次是脾、小肠、胃和股二头肌等,在心脏中表达量最低。在各组织中（除小肠、心、肺）,NR1H3-2的表达量均高于NR1H3-1,其中在肝和皮下脂肪组织中,NR1H3-2的表达量极显著高于NR1H3-1（P<0.01）,在胃中差异达显著水平（P<0.05）。随着日龄的增加,皮下脂肪组织中NR1H3-1和NR1H3-2的表达量整体呈上升趋势,推测该基因对猪的脂肪沉积有调控作用。本试验成功克隆了猪NR1H3基因的两个可变剪接体,并推测其对猪脂质代谢及脂肪生成具有重要的生理作用。 相似文献
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维生素E缺乏对雏鸡脾脏淋巴细胞Caspase-3活性及Caspase-3mRNA表达的影响 总被引：1，自引：0，他引：1
用低维生素E(VE)和添加不饱和脂肪酸的日粮饲喂雏鸡建立VE缺乏雏鸡模型,采用比色法和半定量RT-PCR法分别检测脾脏淋巴细胞Caspase-3活性及Caspase-3 mRNA的表达水平。结果显示,VE缺乏组雏鸡脾脏淋巴细胞Caspase-3活性和Caspase-3 mRNA丰度高于对照组,组间差异均极显著(P<0.01)。结果表明,Caspase-3活性及Caspase-3 mRNA丰度的改变,是VE缺乏雏鸡脾脏淋巴细胞凋亡的调控机制之一。 相似文献
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To assess muscle breakdown during avian coccidiosis, the level of the non-metabolizable amino acid 3-methylhistidine (3MH) was determined in muscle and plasma from turkey poults that received an infection with a field isolate containing a mixture of Eimeria species. The effect of increased levels of parasitism was evaluated at 6 days postinoculation (DPI) in birds receiving 2.5 x 10(4), 1 x 10(5), or 2 x 10(5) oocysts each. The changes in 3MH levels during recovery from acute infection were assessed at 6-29 DPI in animals given 1.9 x 10(5) oocysts per bird. In some experiments, uninoculated birds given the same amount of feed as infected birds (pair fed) were used to determine the impact of feed deprivation on weight loss and 3MH levels. Infected birds had significantly elevated plasma and muscle 3MH at 6 DPI after a single dose of Eimeria oocysts. The plasma and muscle 3MH returned to control levels after 14 DPI. The 3MH levels increased with increased dose of oocysts. Plasma and muscle 3MH levels were well correlated, and an inverse curvilinear relationship between weight gain and plasma 3MH concentrations levels was observed. Plasma and muscle 3MH levels were significantly elevated in pair-fed birds, but 3MH levels in infected birds were increased by 30% over pair-fed birds. The results suggested that muscle breakdown, as assessed by plasma and muscle levels of 3MH, increased during the acute stage of Eimeria infection in turkey poults. 相似文献
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乳牛口服大量β-胡萝卜素对维生素D3及钙磷代谢的影响 总被引：1，自引：0，他引：1
６头健康成年荷斯坦乳牛被随机均分为２组。试验组牛第１周每头口服β－胡萝卜素２３．０ｇ,以后每周每头口服１１．５ｇ,对照组不做任何处理。试验期共８周。结果表明,试验组乳牛血清钙及无机磷水平呈下降趋势;血清维生素Ｄ３活性代谢产物２５－ＯＨ－Ｄ３和１,２５－（ＯＨ）２－Ｄ３均呈现明显下降;至第５周末,血液β－胡萝卜素水平最高,２５－ＯＨ－Ｄ３水平下降非常明显。据此认为,乳牛对β－胡萝卜素的吸收存在一阈值,大剂量的β－胡萝卜素进入机体,可能直接干扰维生素Ｄ３的羟化,使其活性代谢产物呈下降趋势,但由于吸收阈值的存在,其对维生素Ｄ３和骨骼钙磷代谢的干扰作用可得到一定程度的减缓。 相似文献
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Wnt3a在不同毛色羊驼皮肤中的表达和定位 总被引：2，自引：0，他引：2
本研究旨在探索Wnt3a在羊驼皮肤中的表达与定位.以不同毛色的成年羊驼为研究对象,应用荧光定量PCR技术分析不同毛色羊驼皮肤Wnt3a基因的相对表达量,并运用Western blotting及免疫组织化学法对Wnt3a蛋白在不同毛色羊驼皮肤中进行表达和定位研究.结果:荧光定量PCR结果显示棕色羊驼中Wnt3a mRNA相对表达量是白色羊驼的2.9702倍;Western blotting结果表明,羊驼皮肤组织组蛋白提取物中存在相对分子质量约39 ku的产物,棕色羊驼皮肤平均蛋白表达量显著高于白色羊驼;免疫组织化学结果显示Wnt3a在羊驼皮肤毛囊的根鞘和毛球部呈阳性表达,根据光密度值分析得出Wnt3a在棕色和白色羊驼毛囊中的表达差异显著(P＜0.05).通过以上研究显示Wnt3a可能与羊驼毛色形成具有相关性. 相似文献
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为了构建融合表达分子佐剂猪补体C3d与猪流感病毒血凝素(HA)的真核表达载体,论文克隆了猪C3d全长基因与H3N2亚型猪流感病毒的HA基因,并构建了包含3拷贝的C3d和经过改造的HA(替换信号肽并去除跨膜区)的质粒pHA-C3d3.序列分析表明,获得的猪C3d与参考序列相比核苷酸同源性达到99.7%,氨基酸同源性达99... 相似文献
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以河西走廊3个日光温室主栽辣椒（Capsicum frutescens）品种（陇椒2号、陇椒3号和陇椒5号）为材料,研究不同浓度(0、30、60、90、120、180 mmol·L-1)的中性盐NaCl溶液对3个品种的辣椒种子萌发的影响。结果表明,随着盐浓度的增加,3个辣椒品种的种子发芽率、发芽势和发芽指数均不同程度下降,而相对盐害率则逐渐升高,并以陇椒3号的耐盐性最好,当盐浓度为180 mmol·L-1时仍有60.64%的发芽率。盐胁迫对发芽起始时间并无影响,但当盐浓度超过90 mmol·L-1时,发芽高峰期会随着盐浓度的增大而推迟。辣椒叶片内的POD、SOD和CAT的活性均随着浓度升高呈先上升后降低的趋势,以陇椒3号的酶活性升高幅度最大,陇椒2号的上升幅度最小。3个品种辣椒叶片内的MDA含量均随着盐浓度增大逐渐增加,陇椒2号的增加幅度最大,陇椒3号的最小。以上结果反映出3个辣椒品种的萌发和苗期的耐盐能力由强到弱依次为陇椒3号、陇椒5号、陇椒2号。 相似文献
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河西荒漠草原花棒不同栽植密度旱作技术研究 总被引：1，自引：0，他引：1
2004年在甘肃高台县荒漠草原区按照不同密度（2 m×2 m、2 m×3 m、3 m×3 m、3 m×4 m）移栽花棒Hedysarum scoparium 200 hm2,设定样方连续4年观测成活率、分枝数、高度、冠幅、生物量等的变化情况。结果表明,3 m×3 m、3 m×4 m为花棒的最佳移栽密度,在此密度下,花棒生长旺盛,枝条茂密,根系发育良好,丛间物种数增加,盖度提高,固沙效果明显。大于此密度,花棒植株低矮,生长缓慢,丛间原有物种消失,固沙效果较差。 相似文献
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Numbers of airborne bacteria and fungi in calf houses 总被引：1，自引：0，他引：1
Counts of airborne bacteria colony forming particles (BCFP) and fungi were made at intervals throughout one year in three calf houses using a six stage Andersen Sampler. House 1 was insulated, mechanically ventilated and heated, House 2 was insulated and provided with a controlled natural ventilation system, while House 3 was uninsulated with natural ventilation. Each house contained 36 bought-in bull calves. Every six weeks the 12 oldest calves were removed and replaced by 12 four-week-old calves. The mean count of BCFP was highest in House 2 (101.6 X 10(3) m-3) and lower in House 3 (67.6 X 10(3) m-3). The mean count of aerial fungi was significantly lower in House 3 (40.5 X 10(3) m-3) than in Houses 1 and 2 (119.3 X 10(3) m-3 and 127.1 X 10(3) m-3, respectively). The count of aerial BCFP and fungi showed large seasonal fluctuations, but there was a general trend towards lower counts during the winter period. The mean incidence rate of respiratory disease among the experimental calves was 67.7%. The highest mean incidence rate was recorded in House 2, but differences between houses were not significant. The results are discussed in relation to the environmental requirements for raising of calves, and in the light of the current concept of air hygiene as a major predisposing factor in the web of causation of calf respiratory disease. 相似文献
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Elena Fringuelli Lorena Urbanelli Omar Tharuni Patrizia Casagrande Proietti Annalisa Bietta Irit Davidson Maria Pia Franciosini 《Research in veterinary science》2010,89(3):367-372
This is the first paper describing the cloning of pigeon IFN-γ gene (PiIFN-γ) and the analysis of the in vitro expressed recombinant protein. The PiIFN-γ gene was identified by RT-PCR as a 498 bp, fragment coding for a precursor protein of 165 amino acids instead of 164 amino acids, as observed in the other avian species. The recombinant protein was expressed in vitro by an eukaryotic system and the biological properties of the cytokine were tested using a chicken macrophage cell line. The high degree of amino acid and nucleotide identity, shared with the ChIFN-γ, and the fact that the pigeon protein was functional on chicken cells, indicates a cross-reactivity between pigeon and chicken IFN-γ. The detection of the PiIFN-γ could represent an useful instrument in understanding the role played by this cytokine in immune response related to vaccinations and infectious diseases in the pigeon. 相似文献
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旨在研究猪C1QTNF3基因可变剪接体的特性及miR-101通过C1QTNF3基因促进猪脂肪SV细胞成脂分化的机制。本研究采集3头30日龄健康马身猪仔公猪心、肝、脾、肺、肾、胃、股二头肌、腰大肌、皮下脂肪和背部脂肪组织样品,首先利用RT-PCR技术和生物信息学方法对C1QTNF3基因的不同转录本进行扩增和生物学特性分析,采用qRT-PCR技术检测C1QTNF3基因不同转录本在猪组织中的表达变化。随后,利用生物信息学预测发现,C1QTNF3上游的调控因子是miR-101,采用双荧光素酶报告试验验证miR-101对C1QTNF3的调控作用。最后,利用油红O染色和qRT-PCR技术检测过表达miR-101对猪脂肪SV细胞成脂分化的影响。基因克隆得到猪C1QTNF3存在两个转录本C1QTNF3和C1QTNF3-1,其中C1QTNF3-1为新鉴定的转录本;测序分析显示,与C1QTNF3相比,C1QTNF3-1的第1外显子区域缺失了219个碱基,少编码73个氨基酸。进化树分析显示,猪C1QTNF3和C1QTNF3-1蛋白序列与人和马来亚穿山甲等物种的亲缘关系较近。C1QTNF3和C1QTNF3-1在猪各组织中均有表达,且在各组织中C1QTNF3-1的表达量均极显著高于C1QTNF3（P<0.01）。过表达miR-101极显著下调C1QTNF3 3'UTR区域的荧光素酶活性（P<0.01）和C1QTNF3 mRNA的表达（P<0.01）,同时增加了脂肪细胞成脂分化关键基因PPARγ、C/EBPβ、SREBP-1c和FABP4的mRNA表达量（P<0.01）。本试验成功克隆了猪C1QTNF3基因的两个可变剪接体,其中C1QTNF3-1在猪不同组织中均高表达,推测该转录本为基因发挥功能的主要亚型。并深入研究了C1QTNF3基因的上游调控因子,揭示了miR-101靶向C1QTNF3促进成脂分化的机制,丰富了C1QTNF3的生物学作用和调控网络。 相似文献
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为探讨葡萄糖氧化酶对种公猪生殖性能的影响,选择18～24月龄、体重150～200 kg健康长白种公猪25头,分为5组,每组5个重复.对照组饲喂基础日粮,试验1、2、3、4组分别在基础日粮中添加1、2、3、4kg/t的葡萄糖氧化酶.试验期28 d.结果表明:试验3、4组公猪血清中雌二醇含量显著高于对照组(P＜0.05),... 相似文献
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C3 was obtained from bovine serum by polyethylene glycol precipitation and chromatography on DEAE-Sephadex A-50, CM-Sephadex A-50 and Sephacryl S-200. The protein has a molecular weight of 183,000 (alpha-chain 114,000 and beta-chain 69,000). A CVF-induced bovine C3 convertase (Sepharose-CVF.Bb) cleaved C3 into C3a (11,000) and C3b (172,000) as shown by SDS-polyacrylamide gel electrophoresis. Isoelectricfocusing of C3 demonstrated at least three electrophoretic variants with pI 6.55–6.85. The isolated protein promoted the formation and action of a C3 convertase in the presence of purified bovine factors B and D. A monospecific antiserum prepared in rabbits failed to cross react with human C3 or CVF. C3c was identified as a contaminant during the isolation of C3. 相似文献