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1.
Benfuresate (2-3-dihydro-3,3-dimethylbenzofu-ran-5-yl ethanesulfonate) is a selective herbicide for the control of purple nutsedge in cotton. Under outdoor conditions, purple nutsedge was sensitive to benfuresate incorporated in soil up to eight days after initiation of shoot sprouting from the tuber. Older seedlings recovered from the damage. During the period of susceptibility to benfuresate, young shoots more sensitive than the roots. Under controlled environmental conditions, benfuresate applied directly to apical buds developing from the tuber caused severe damage to the treated bud and induced abrupt development of axillary buds. Negligible amounts of the applied herbicide were translocated from the treated part to the other buds and roots. Application of the herbicide to fully developed leaves had no effect, probably because of its rapid metabolism and low basipetal mobility. Its relatively high volatility may also contribute to its low foliar post-emergence activity. Tubers also absorbed herbicide vapours. Root uptake of 14C-benfuresate resulted in a rapid accumulation of 14C in the shoot, which had no effect on the purple nutsedge plant, regardless of concentration. The herbicide is rapidly converted, mainly to a non-phytotoxic polar product. These results may explain the high sensitivity of the weed to benfuresate at early growth stages, and the lack of sensitivity in mature plants. 相似文献
2.
Experiments were conducted in a growth cabinet to investigate the absorption and translocation of 14C-3, 6-dichloropicolinic acid by Cirsium arvense (L.) Scop. (Canada thistle, creeping thistle), a sensitive species. Applications were made, either to the middle four leaves of 12-cm-tall vegetative plants grown under low (40%) and/or high (>95%) relative humidity (r.h.), or to four upper or lower leaves of 30-cm-tall flowering plants grown under low r.h. Following application to vegetative plants, absorption and translocation of 14C-3,6-dichloropicolinic acid was rapid and was approximately doubled by high r.h. High r.h. increased the amount of radioactivity retained by the treated leaves or translocated to the shoots but did not affect greatly the amount retained in the roots. The herbicide was highly mobile, with over half of that absorbed, translocated out of the treated leaves after two days. The apex accumulated most of the radioactivity, while approximately 8% was recovered from the roots. The absorption and translocation patterns were similar to those reported in the literature for picloram in C. arvense. Absorption of 3,6-dichloropicolinic acid was greater in vegetative than in flowering C. arvense plants, and placement of herbicide on lower leaves tended to decrease the amount of radioactivity recovered from shoot apex and increase the amount recovered from the roots. Approximately 15% of the applied radioactivity could not be recovered from treated plants by 2 days after treatment. 相似文献
3.
In the present study, 98.8 mm of bentazon was applied to 3‐leaf stage rice seedlings. Two tolerant lines, M202 and cv. TNG67, showed slightly visible injury, photosystem II inhibition, as well as a low level of lipid peroxidation 7 days after application compared with the susceptible lines. Further physiological study of the mechanism of differential tolerance among Japonica and Indica types indicated that, although the tolerant Japonica lines M202 and cv. TNG67 absorbed more 14C‐bentazon, most of the 14C remained in the treated leaf or translocated to older leaves. However, two susceptible lines, FSK (Japonica) and IR36 (Indica), absorbed less 14C‐bentazon throughout the experiment, and most of the 14C was translocated from the treated leaves to younger leaves, which might result in the death of developing tissues. In addition, more bentazon residue and less polar metabolites were detected in these two susceptible lines. It is proposed that the higher tolerance of lines M202 and TNG67 to bentazon could be mainly due to a higher rate of metabolism of this herbicide, and partially due to less translocation to developing tissues. 相似文献
4.
Translocation of the antiblast compound, carpropamid, was investigated in rice using [14C]carpropamid. When applied to the seed, carpropamid was not only readily absorbed but was translocated to different parts of the seedlings emerging from treated seeds. A substantial portion of fungicide appeared to be exuded onto the leaf surface. In 21‐day‐old plants grown from [14C]carpropamid‐treated seeds, 27.2% of the radioactivity isolated from leaves was present on the surface of lamina. This exuded fraction is probably responsible for its action as a fungal anti‐penetrant compound. Following 30‐min root dipping of 14‐day‐old seedlings, carpropamid was rapidly absorbed and translocated throughout the seedling. Its intra‐laminar distribution was uniform as determined by autoradiography. Only a small fraction (<2%) of fungicide applied to the foliage was translocated beyond the site of application within the treated leaf. Translocation was primarily apoplastic. Approximately 54% of the radioactivity recovered from leaves was in the form of carpropamid. At least seven radiolabelled metabolic products were observed by TLC. Only 8.3% of radioactivity applied through the seeds could be recovered from 21‐day‐old seedlings. © 2001 Society of Chemical Industry 相似文献
5.
Seedlings of Solanum scabrum Mill. and Solanum ptycanthum Dun. were treated with [14C]ethalfluralin (N-ethyl-α,α,α-trifluoro-N-(methylallyl)-2,6-dinitro-p-toluidine) and [14C]trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine) supplied in nutrient solution to determine the basis for differences in response by these two species to these two herbicides. Plants of S. scabrum absorbed more [14C]ethalfluralin and [14C]trifluralin than plants of S. ptycanthum. During the first 24 h, S. scabrum seedlings, but not S. ptycanthum seedlings absorbed more [14C]ethalfluralin than did plants treated with [14C]trifluralin. More [14C]ethalfluralin than [14C]trifluralin was found in the shoots of plants of both species. Seventy-two hours after treatment with [14C]herbicides, the conversion to water-soluble metabolites was greater for [14C]ethalfluralin than for [14C]trifluralin. In the shoots of plants from both species an average of nearly 55% of the 14C recovered was found in the water-soluble fraction following [14C]ethalfluralin treatment whereas an average of only 40% was found in the water-soluble fraction following [14C]trifluralin treatment. 相似文献
6.
Ian C. BurkeJohn W. Wilcut 《Pesticide biochemistry and physiology》2003,76(2):37-45
Greenhouse and laboratory experiments were conducted to determine the effect of imazapic on the herbicidal activity of clethodim on goosegrass. Imazapic did not affect absorption of [14C]clethodim by goosegrass. Averaged across the two treatments of clethodim alone and clethodim plus imazapic, absorption was 36 and 89% of applied [14C]clethodim at 0.5 and 96 h, respectively. The majority of [14C]clethodim (79% of applied) was absorbed by 24 h. Translocation of 14C was not affected by imazapic, and 3.6% of applied 14C had translocated into the portion of the shoot below the treated leaf at 96 h after treatment. Metabolism of clethodim was not affected by the presence of imazapic. Three major metabolites of clethodim were detected in treated tissue at all harvest intervals. The majority (58%) of [14C]clethodim was converted to a relative polar metabolite form 96 h after treatment, whether clethodim was applied alone or in the presence of imazapic. One day after treatment, the photosynthetic rate in plants treated with imazapic decreased below the rate in the non-treated check, and was less for 8 days, the duration of the study. These data suggest that the antagonism of clethodim by imazapic may be caused by imazapic reducing the photosynthetic rate of goosegrass and therefore the sensitivity of ACCase to clethodim. 相似文献
7.
Glyphosate at 2 kg/ha was more effective in reducing regrowth of purple nutsedge (Cyperus rotundus L.) scapes at 90% than at 50% relative humidity (r.h.), and more effective at ?2 bars than at ?11 bars of plant water potential. Regrowth of treated plants subjected to water potentials of ?1 to ?8 bars was reduced 54–60% while at ?11 bars growth inhibition was only 34%. A time interval of as little as 8 h between application and excision was sufficient to give 47% reduction in regrowth at 90% r.h. None of the treated plants, except those clipped immediately after application, produced new shoots from the basal bulb, while all the untreated control plants produced one or more new shoots. Experiments using 14C-glyphosate substantiated these results. Three times more 14C-label was translocated into the underground parts of nutsedge at 90% than at 50% r.h. Twice as much translocated at ?2 bars than at ?11 bars of water potential. 相似文献
8.
The concentrations of haloxyfop in nutrient solution required to reduce the total plant dry weight of soybean (Glycine max L. Merr. ‘Evans’), red fescue (Festuca rubra L. ‘Pennlawn’), and tall fescue (Festuca arundinacea Schreb. ‘Houndog’) by 50% (GR50) were determined. The GR50) values for soybean, red fescue and tall fescue were 76 μM, 3μM and 0.4 μM, respectively. The reduction in growth in roots and shoots of soybean was similar. In contrast, the relative reduction in root tissue weight was greater than that for foliar tissue in both grass species. The amount of 14C-haloxyfop in soybean roots or shoots was higher than in red fescue or tall fescue. Red fescue accumulated less haloxyfop in the foliage than in the roots. On the other hand, similar amounts of 14C-haloxyfop accumulated in both organs in both soybean and tall fescue. 14C-haloxyfop appeared to be actively absorbed by the roots of all species. Soybean absorbed more nutrient solution, but utilized it less on a per gram dry matter produced basis than the grass species. Differences in the uptake and translocation of haloxyfop by roots do not account for differences in tolerance between species. However, a higher level of retention of haloxyfop in the roots of red fescue than in tall fescue may provide the former with an additional selectivity advantage under conditions where there is significant root exposure to the herbicide. 相似文献
9.
Absorption, translocation and distribution of 14C-glyphosate were examined in Agropyron repens (L.) Beauv. plants growing under field conditions in the autumn. Glyphosate absorption did not increase beyond 3 days after application, whereas translocation to the rhizomes continued up to 7 days after application. The translocated glyphosate accumulated more in new rhizomes than in older parts of the rhizomes. Ten per cent of the glyphosate translocated out of the treated shoot was recovered in younger shoots 7 days after application. Plants harvested the following spring contained less than 20% of the glyphosate originally applied. Although a growth cabinet experiment indicated that 34% of the glyphosate in the rhizomes of treated plants could be remobi-lized into new aerial shoots, considerably less was recovered in new, aerial shoots in the spring in the field-grown plants. Freezing experiments showed that glyphosate translocation to the rhizomes was only prevented when cold treatment caused visible damage to A. repens foliage. 相似文献
10.
Low rates of picloram in mixture with glyphosate provided a rapid enhancement of the onset of injury to the shoots of Cirsium arvense (Canada thistle or creeping thistle) under field (0.07+1.0 and 0.07+1.5 kg ha?1) and greenhouse (0.035+0.42 and 0.07+0.84 kg ha?1) conditions. Picloram slightly reduced the amount of 14C-glyphosate absorbed at 24 and 48 but not 72 h after treatment. Movement of 14C-glyphosate from the treated leaves to the shoot apex, remainder of the shoot and roots was reduced in the presence of picloram. Necrosis of the treated leaves above the treated spots was evident, presumably indicating acropetal movement of either or both herbicides. With the picloram + glyphosate mixtures there was increased shoot regrowth over glyphosate alone at 1 year after treatment under field, and with certain mixtures at 18 days and 4 weeks after treatment under greenhouse conditions. Following application of the mixtures, accumulation of glyphosate in the shoots may be responsible for the enhanced onset of shoot injury while failure of enough glyphosate to translocate to, and cause death of, the roots may be responsible for the increased shoot regrowth over glyphosate alone. 相似文献
11.
The differential tolerance of resislant creeping red fescue (Fes- tuca rubra L, var, rutra) and susceptibie reed canarygrass (Pha- laris arundinacea L.) seedlings to glyphosate [N-(phosphono- meihyDglycine) was confirmed under growth chamber condilions. The absorption, transiocation and metabolism of 14C-glyphosate was examined in both species to determine if differences in these processes could account for the observed selectivity, Creepmg red fescue actually absorbed more glyphosate than did t-eed canarygrass, and both species rapidly translocated the herbicide throughout their respective tissues. No metabolism of glyphosate was detected in either species. Differential interception and retention of the glyphosate spray can probably be eliminated as possible selectivity mechanisms. The relative tolerance of creeping red fescue to glyphosale appears to be related to its ability lo regenerate roots and shoots from the crown of the plant, but the mechanism of resistance remains obscure. 相似文献
12.
Glyphosate resistance in common ragweed (
A
mbrosia artemisiifolia L.) from Mississippi,USA 
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下载免费PDF全文 Glyphosate is one of the most commonly used broad‐spectrum herbicides over the last 40 years. Due to the widespread adoption of glyphosate‐resistant (GR) crop technology, especially corn, cotton and soybean, several weed species have evolved resistance to this herbicide. Research was conducted to confirm and characterize the magnitude and mechanism of glyphosate resistance in two GR common ragweed ( A mbrosia artemisiifolia L.) biotypes from Mississippi, USA. A glyphosate‐susceptible (GS) biotype was included for comparison. The effective glyphosate dose to reduce the growth of the treated plants by 50% for the GR1, GR2 and GS biotypes was 0.58, 0.46 and 0.11 kg ae ha?1, respectively, indicating that the level of resistance was five and fourfold that of the GS biotype for GR1 and GR2, respectively. Studies using 14 C‐glyphosate have not indicated any difference in its absorption between the biotypes, but the GR1 and GR2 biotypes translocated more 14 C‐glyphosate, compared to the GS biotype. This difference in translocation within resistant biotypes is unique. There was no amino acid substitution at codon 106 that was detected by the 5‐enolpyruvylshikimate‐3‐phosphate synthase gene sequence analysis of the resistant and susceptible biotypes. Therefore, the mechanism of resistance to glyphosate in common ragweed biotypes from Mississippi is not related to a target site mutation or reduced absorption and/or translocation of glyphosate. 相似文献
13.
A. J. SANAD 《Weed Research》1971,11(4):215-223
Studies of the uptake and translocation of 14C-labelled 2, 4-D, MCPA and aminotriazole in Agrostemma githago L. and Tussilago farfara L. clarified the behaviour of the herbicides in both species. In A. githago, MCPA was more freely mobile than 2,4-D after application to the leaf; it was distributed in the plant more rapidly and in greater quantity. Similarly, following root uptake MCPA was transported in the shoot in greater amounts than was 2,4-D. There is a clear relationship between the susceptibility of A. githago to MCPA and the mobility of the herbicide in the plant. In T. farfara, 2,4-D and aminotriazole applied to the leaves were equally well absorbed and relatively rapidly translocated. During the period up to 72 h the amounts of herbicide in the plant increased to similar levels; after that, 14C activity in plants treated with 2,4-D fell slightly whereas there was further accumulation of aminotriazole. Following uptake through the roots, translocation and accumulation in the leaves were considerably greater with aminotriazole than with 2,4-D. The lack of accumulation of 2,4-D could be a factor in the resistance of T. farfara to this herbicidie. Recherches sur l'absorption et la migration d'herbicides marqués au 14 C dans Agrostemma githago L. et Tussilago farfara L. 相似文献
14.
Radioactive 2,4,5-T was applied to the leaves of small Rubus precerus (P. J. Muell.) plants grown in pots in the glasshouse in amounts ranging from 24 μg to 55.8 μg per leaf. The amount of 2,4,5-T absorbed and translocated was measured 6h and 24h later. The herbicide was translocated throughout the plant within 6h and the concentration in the various plant parts increased between 6h and 24h. More 2,4,5-T was absorbed at high concentrations than at low concentrations although the percentage absorbed in 24h decreased from 13% to 6% as the concentration was increased Translocation of 2,4,5-T-1-14C was variable, although the amount translocated inereased as the amount applied was increased, and was closely correlated to the amount of herbicide absorbed by the leaves. 相似文献
15.
Growth chamber experiments were conducted in order to study the absorption, translocation and activity of glyphosate when applied to roots with aqueous solution avoiding any glyphosate–substrate interaction. Corn seedlings at the first leaf stage were set up in individual graduated cylinders containing different solutions of 14 C-glyphosate (0–30 mg ae kg−1 ). After 26 h of root exposure, plants were transferred to fresh nutrient solution and grown for the next 5 days. After harvest, plants were separated into seed, root, mesocotyle, coleoptile, cotyledon, first leaf and all new leaves (apex), and quantified 14 C radioactivity contained in each part. Glyphosate uptake was only 11% of the theoretical mass flow into the plant. The amount of glyphosate translocated from roots was positively correlated with plant uptake ( P < 0.01). Total plant fresh weight presented a logistic response to glyphosate amounts, including a growth stimulant effect (hormesis), when plants absorbed less than 0.6 µg. The treated plants presented a normal pattern of glyphosate allocation, with the apex the principal sink, accumulating more than 38% of mobilized glyphosate. When corn plants absorbed more than 0.6 µg they showed a decrease in growth. The relatively high glyphosate quantities allocated in the new leaves showed the relevance of the symplastic pathway in the translocation process for root absorbed glyphosate. 相似文献
16.
Subhash C. Domir 《Pest management science》1980,11(4):418-422
Radiolabelled daminozide and maleic hydrazide (MH) were injected into American elm seedlings, kept in nutrient solution, to determine their translocation pattern and metabolic fate. Both compounds were rapidly translocated to all parts of the plant. After 21 days, 13% of the applied 14C was exuded into the nutrient solution from the roots of the plants treated with MH. Using gel-filtration and thin-layer chromatographic techniques, it was determined that daminozide did not form any metabolite, and that MH was converted into a MH-sugar complex. A significant amount of 14C was unextractable from the plant tissue. 相似文献
17.
The uptake and translocation of 14C-ring-labeled asulam (methylsulfanilcarbamate) and bromacil (5-bromo-3-sec-butyl-6-methyluracil), were compared after root application to maize (Zea mays L.) and bean (Phaseolus vulgaris L.). Autoradiographs showed the distribution of bromacil throughout these and other plant species, and the retention of asulam in the roots. The recovery of both compounds in quantitative radioassays was between 90 and 100%. The absorption of bromacil and asulam was rather similar. Absorption of bromacil increased up to 20% of the applied dose in bean plants after 2 days of exposure, and up to 11% in maize plants after 4 days. Absorption of asulam in bean plants was 22% of the applied dose after 2 days, and 8% in maize plants after 4 days. The pattern of distribution of bromacil and asulam was completely different. After 4 h of exposure of the roots about half of the absorbed bromacil had accumulated in the shoots, while two-thirds or more was translocated to the shoots after exposure periods of 1 to 4 days. Not more than one-eighth of the absorbed asulam was found in the shoots. In consequence, the bromacil content in the transpiration stream relative to that in the ambient solution was much higher than that of asulam. The leakage of asulam from bean and maize roots into herbicide-free nutrient solution was lower than that of bromacil. The reasons for these differences are not yet clear. There was only some metabolism of asulam in maize, but not in bean plants. No metabolites of bromacil were detected in the two plant species. 相似文献
18.
The pattern and extent of 14C-glyphosate [N-(phosphonomethyl)glycine] translocation from the treated leaf and metabolism of 14C-glyphosate were studied in field bindweed (Convolvulus arvensis L.), hedge bindweed (Convolvulus sepium L.). Canada thistle [Cirsium arvense (L.) Scop.] tall morning glory [lpomoea purpurea (L.) Roth.] and wild buckwheat (Polygonum convolvulus L.). 14C was translocated throughout the plants within 3 days with accumulation in the meristematic tips of the roots and shoots evident. Cross and longitudinal sections of stems and roots showed that the 14C was localized in the phloem. Field bindweed translocated 3–5% of the applied 14C from the treated leaf, hedge bindweed 21.6%, Canada thistle 7.8%, tall morningglory 6.5%, and wild buckwheat 5%. Field bindweed, Canada thistle, and tall morningglory metabolized the parent glyphosate to aminomethylphosphonic acid to a limited extent. This metabolite made up less than 15% of the total 14C. Of the total 14C applied to excised leaves, 50% had disappeared within 25 days. 相似文献
19.
MENASHE HOROWITZ 《Weed Research》1973,13(2):200-208
Summary. Small plants of Sorghum halepense (L.) Pers. were planted in the field and grown without competition from weeds and crops for 2½ years. The plant spread by means of subterranean rhizomes from which aerial shoots developed at increasing distances from the plant centre, and by tillering around these aerial shoots to form clumps. No preferential direction of expansion was detected and established patches developed an approximately circular shape. Aerial growth stopped completely in the cold season. During the warm season the mean area increment amounted to 1·3 m2/month and was similar in both years of observations. After 2½ years of growth, patches had extended up to 3·4 m from the initial sprig and had a mean area of 17 m2. In the second summer of growth, shoot density reached 190/m2 and mean seed production was 84 g, or 28 000 seeds per plant. About 80% of rhizome dry weight was present in the upper 20 cm of soil and rhizomes did not penetrate deeper than 40 cm. About 80% of rhizome dry weight was present within 1 m radius from the plant centre. Croissance dans l'espace de Sorghum halepense (L.) Pers. 相似文献
20.
The comparative uptake and metabolism of 14C-labeled 2-(3,4-dichlorophenyl)-4-methyl-1,2,4-oxadiazolidine-3,5-dione (methazole), a herbicide, in prickly sida (Sida spinosa L.) and cotton (Gossypium hirsutum L.) were investigated as physiological bases for herbicidal selectivity, using thin layer chromatography, autoradiography, and liquid scintillation counting. Prickly sida and cotton readily absorbed and translocated 14C from nutrient solution containing [14C]methazole. Only acropetal translocation of 14C was observed. Methazole was rapidly metabolized to 1-(3,4-dichlorophenyl)-3-methylurea (DCPMU) and other metabolites by both species. Although metabolism appeared to be qualitatively the same, quantitative differences between species were evident. Methazole was converted to DCPMU (also phytotoxic) more readily by prickly sida than cotton; however, DCPMU was more readily detoxified to 1-(3,4-dichlorophenyl) urea (DCPU) by cotton than prickly sida. More 14C per unit weight was present in the prickly sida shoots than in cotton shoots. Also, a larger portion of the methanol-extractable 14C was herbicidal in the shoots of prickly sida than of cotton. Thus, the differential tolerances of prickly sida and cotton to methazole may be explained, in part, by differential uptake and metabolism of methazole and DCPMU. 相似文献