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1.
The effects on growing pigs of substituting 4.5 and 9 % of soy bean meal with a corresponding amount of single cell protein produced from sulphite spent liquor in a diet based on cereals and fish meal have been studied. The concentration of lignosulphonic acids in the single cell protein product was found to be 0.6 % ± 0.2 % (m. ± s). No differences in the weight gain, feed conversion ratio or fat thickness of the pigs, as compared with the controls, were observed when fed single cell protein-containing diets from about 29 to 80 kg in the course of 11 weeks. Nor were any effects found on addition of 0.15 % of methionine to the diets.  相似文献   

2.
The inhibitory effect of peptide-precipitating lignosulphonic acids on the proteolytic activity of pepsin in vitro has been studied. When an unfractionated lignosulphonic acid preparation was used, total inhibition of the activity of 5 µg crystalline pepsin was obtained by addition of about 15 mg of lignosulphonic acids.The greatest inhibitory effect was seen with the high-molecular weight lignosulphonic acids, but an effect was also seen with middle-and low-molecular weight lignosulphonic acids.A reduction in the inhibitory effect was seen when the experiments were performed in the presence of arginine. The inhibition mechanism is discussed in relation to the peptide-precipitating ability of the lignosulphonic acids.No obvious protection against the appearance of stomach ulcers in four pigs receiving an ulcerogenic diet was seen.  相似文献   

3.
Lignosulphonic acids in dialysed sulphite spent liquor and purified lignosulphonic acids were subjected to gel chromatography on Sephadex G-75, G-100 and G-200 and the fractions tested for peptide-precipitating ability. About 56 % of the total lignosulphonic acids in the dialysed sulphite spent liquor had estimated molecular weights above 90000 and about 72 % above 44000. About 94 % of the purified lignosulphonic acids had molecular weights above 90000 and the remaining 6 % had above 36000. The major peptide-precipitating activity of the lignosulphonic acids was due to fractions with molecular weights in excess of 90000. The percentage of peptides in the peptide-lignosulphonic acid precipitates was found to be 80–90. The molecular weights of the peptides used were found to have an upper limit of about 20000. The lower limit for molecular weights of lignosulphonic acid-precipitating peptides is estimated to be below 6000.Keyword: gel filtration, lignosulphonic acids, molecular weight, peptide-precipitating ability  相似文献   

4.
The effects of incubation time and incubation temperature on precipitation zones of peptide-lignosulphonic acid complexes in agar plates are studied in relation to the mathematical dependence of zone diameter as a function of the concentrations of lignosulphonic acids in serial 2-fold dilutions. Optimal conditions are given for obtaining regression lines with not significant second degree coefficients under defined conditions.A diffusion unit, proposed as a measure of peptide-precipitating lignosulphonic acids in aqueous solution, is defined. The possibility is discussed of using the described procedure as a micro quantitative method for the determination of peptide-precipitating lignosulphonic acids in aqueous solution.  相似文献   

5.
The effects of lignosulphonic acids on colicins, coliphages, different antibiotics, ß-toxin of Staphylococcus aureus, lecithinases of Clostridium perfringens and of Bacillus cereus, hemolysins of Bacillus cereus and Escherichia coli, the bactericidal effect of lysolecithin and on specific and naturally occurring serum proteinase inhibitors have been studied. It was found that lignosulphonic acids distinctly inhibit the biological activities tested, with the possible exceptions of the inhibitory effects of serum antiproteinases, naturally occurring inhibitors in serum, and the bacteriolytic effect of coliphages. The inhibiting effects are discussed in relation to the use of animal fodder containing lignosulphonic acids, and to the appearance of a selected microbial flora in sulphite spent liquor recipients.  相似文献   

6.
It has been shown that lignosulphonic acids have an inhibitory effect on various animal and microbial proteinases. The activity of 0.005 mg swine trypsin, 0.007 mg bovine a-chymotrypsin and the proteinase activity in 0.004 ml of jejunum content from pig is inhibited 70–75 % by 0.25–0.30 ml of a solution containing 250 diffusion units of peptide-precipitating lignosulphonic acids per 50 µl.The inhibitory effect is discussed in relation to the use of animal foodstuffs containing lignosulphonic acids, and in relation to biochemical reactions taking place in ecosystems in which lignosulphonic acids comprise an important part.  相似文献   

7.
The fermentation of various sugars by Gandida utilis in the presence of purified peptide-precipitating lignosulphonic acids has been studied. An inhibitory effect on the fermentation of sucrose, glucose, xylose and an equimolar mixture of glucose and fructose was found, the effect being most pronounced for xylose. Inhibition of the fermentation of xylose was seen when 0.5 % lignosulphonic acids were included, but not when 0.25 % were used. A difference in the inhibitory effect on the fermentation of sucrose and an equimolar mixture of glucose and fructose was observed, and the result is discussed in relation to a possible inhibitory mechanism of the lignosulphonic acids.The peptide-precipitating ability of the lignosulphonic acids seemed to be little influenced during the fermentation of the sugars by Gandida utilis.Keyword: lignosulphonic acids, fermentation, sugars, Gandida utilis  相似文献   

8.
An experiment was conducted to determine the effects of 1) long-acting oxytetracycline injection at market arrival, 2) feed or water access at the auction market and 3) receiving diet management on commingled feeder pig performance. A total of 288 commingled feeder pigs transported over 1,000 km after market management treatments were used in two trials. Pigs given access to feed and water (FW) at the market weighed more (P less than .001) following marketing and transport than pigs given water only (W). Pigs given neither feed nor water (N) were intermediate in arrival weight (19.7, 18.7 and 18.9 kg). There was no effect (P greater than .1) of feed and water offering at the market on final weight (96.6, 95.9 and 96.6 kg), overall average daily gain (.70, .69 and .70 kg/d), overall gain/feed (.31, .31, .32) or percentage death loss (1.0, 2.1 and 4.2%). Injection of long-acting oxytetracycline upon arrival at auction market had no effect (P greater than .1) on pig weight following marketing and transport (19.0 vs 19.2 kg), final weight (96.1 vs 97.0 kg), percentage death loss (3.4 vs 1.4%) or percentage of pigs treated (3.4 vs 4.9%). Compared to providing ad libitum access to feed, restricting feed intake by floor feeding for the first 7 d post-arrival reduced (P less than .01) rate of gain for the first 9 d (-.04 vs. .08 kg/d), but overall there was no effect (P greater than .1) on daily gain (.70 vs .69 kg/d) or gain/feed (.31 vs .31).  相似文献   

9.
本研究旨在研究降低全混合日粮蛋白质水平对育肥期绵羊生长性能、胴体性状及肌肉脂肪酸组成的影响.试验将32只平均体重为(38.14±0.45)kg的育肥期绵羊随机分为2组,每组8个重复,每个重复2头.两组绵羊在为期10周的试验期中分别饲喂蛋白质水平为13.5%和8.5%的全混合日粮.结果:将全混合日粮蛋白质水平从13.5%...  相似文献   

10.
The precipitation of peptide- and protein-lignosulphonic acid complexes can be demonstrated and studied in agar plates in the form of precipitation zones. The effects on the precipitates caused by variations in incubation temperature, incubation time, concentration of the reagents and in pH in the mixture of reagents, are described, and the nature of the peptide-lignosulphonic acid bond is discussed. The central lysis zones which appeared in some precipitation zones were found to be probably caused by excess of lignosulphonic acids. The possibility of developing the agar precipitation method described as a direct micro quantitative procedure for the determination of certain lignosulphonic acids in aqueous solution is suggested.  相似文献   

11.
The effects of dietary level of lysine and of level and source of CP on voluntary feed intake, growth performance, plasma free amino acids, and carcass characteristics were investigated in a study involving 60 female and 60 castrated male Large White finishing pigs (from 42 to 101 kg live weight) with ad libitum access to feed. Six treatments were compared according to a 2 x 3 factorial plan, with two levels of lysine (.55 and .65% selected below the recommended levels for both sexes) and three types of CP (N x 6.25) supply: a 13% CP diet based on wheat, peanut meal and soybean meal; a 15.6% CP diet providing the same amino acid pattern as that of the basal diet; and a 15.2% CP diet containing the same levels of essential amino acids as the 13% CP diet, with the addition of glutamic acid as a source of nonessential amino acids. By maintaining a constant amino acid pattern separate changes in dietary lysine and CP levels resulted in a relative independency of their effects on feed intake, growth performance, and body composition. Muscle gain increased with supplementary lysine, with a lower response at the lower CP level (13%). At the same level of lysine (.55 or .65%), increasing protein content from 13 to 15.6% did not affect feed intake, but growth rate was lower and feed/gain was increased, partly because of an additional energy cost resulting from catabolism of excess protein.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

12.
Rice bran, a by-product of the rice industry, is available for animal feeds. However, it has not been a common part of poultry rations in Argentina. Hydrolytic and oxidative rancidity development, phytate content, enzyme inhibitor, and high fiber content are the most important antinutritive factors cited as limiting factors for its use. An experiment utilizing 1-d-old male broiler chicks was conducted to determine the responses of zootechnical and bone mineralization parameters to diets with different concentrations of rice bran. The feed conversion and tibia ash were more sensitive than weight gain to detect antinutritive factors in rice bran. High concentrations of rice bran (in excess of 20%) produced a significant reduction in body weight. Furthermore, feed conversion and bone mineralization variables were impaired by feeding 10% rice bran diets. The adverse effects of rice bran on weight gain, feed conversion, and mineralization in the current studies suggest that rice bran should be included in broiler diets at a level between 10 and 20% if strategies are not used to decrease the antinutritive activity.  相似文献   

13.
The effects of crude protein (CP) concentration in feed using soybean meal as its source in the early fattening period on the levels of free amino acids (FAAs) and dipeptides in the blood, and the levels in the M. longissimus thoracis after slaughter were studied in Japanese Black steers. Sixteen steers were divided into four groups and given feed with a CP content of 12, 14, 16, or 18% of dry matter (DM) from 10 to 20 months old, and they were fed with the same level of CP (13.5–13.9% of DM) until slaughter at 30 months of age. There was no significant difference in the weight gain, carcass weight or marbling score between the groups. Concerning the serum FAA and dipeptide contents at 20 months of age, the alanine, tyrosine and tryptophan levels decreased, while the carnosine (Car) level increased, with increases in the CP level in the feed. Although there were no significant differences in the FAA contents of the Longissimus thoracis muscle between the groups, the Car content decreased with increases in the feed protein level.  相似文献   

14.
Scientific feeding experiments were carried out with 5 castrated male pigs and 4 female animals of the genetic origin "Camborough" in the live weight range between 30 and 100 kg with a subdivision at 60 kg. In each test group the animals were fed individually and received rations with approximately equal contents of protein, amino acids, minerals and vitamins but different energy contents. Energy intake was adjusted to 3 levels of 100%, 85% and 70% of semi-ad-libitum intake. Apart from that, the digestibility of the feed mixtures used in the 2 fattening periods was determined. Having reached a live weight of 100 kg, 7 representative animals per group were slaughtered and a number of slaughtering parameters were registered. In all three test groups the daily live weight gain over the complete fattening period was very high (846, 819 and 778 g). Though the weight gain in group 3 with 70% of the energy supply of group 1 is significantly lower than in the other two groups, it is, however, only 70 g lower than in group 1. Consequently, the feed expenditure (2.6 kg mixed feed per kg live weight gain) in group 3 is by 20% lower than in group 1, in which the feed expenditure is 3.2 kg mixed feed per kg live weight gain. There were no significant differences on the weight gain performances and the registered carcass parameters between sows and castrated male pigs. Observed deviating tendencies are discussed.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

15.
In a pilot experiment, bullocks were given diet with a sodium monensinate supplement as the Rumensin preparation. The dose of the supplement was 125 mg per head/day and the medicated feed was given to the bullocks for 14 months. The daily weight gain of the tested bullocks increased by 3.85% and feed consumption decreased by 7.12%. The meat of the ten bullocks examined (m. longissimus dorsi) showed no statistically significant changes in the basic chemical composition as compared with the meat of ten control bullocks. In the tested group the content of hydroxyproline in meat decreased by 35.14% (from 0.12 to 0.08 g per 100 g of fresh tissue - p less than 0.05). The content of glycine decreased by 8.82% (from 3.63 to 3.31 g per 100 g of dry matter - P less than 0.01). The content of alanine decreased by 5.86% (from 5.12 to 4.82 g per 100 g of dry matter - P less than 0.05). As to essential amino acids, the content of valine in the meat of the tested bullocks increased by 4.76% (from 3.99 to 4.18 g per 100 g of dry matter - P less than 0.05). Sodium monensinate exerted a favourable influence on weight gains and nutrient conversion in the bullocks. It had no effect on the basic chemical composition and no marked influence on the content of amino acids, except hydroxyproline and, particularly, essential amino acids in the meat of the tested bullocks. A reduction in the content of hydroxyproline signalizes higher-value proteins.  相似文献   

16.
1. The effects of increasing concentrations (0, 40, 80 or 120 g/kg) of bacterial protein meal (BPM) and bacterial protein autolysate (BPA) grown on natural gas on growth performance and carcase quality in broiler chickens were examined. 2. Adding BPM to diets reduced feed intake and improved gain: feed from 0 to 21 d and overall to 35 d, but did not significantly affect weight gain compared to the soybean meal based control diet. 3. Increasing concentrations of BPA significantly reduced growth rate, feed intake, gain: feed, carcase weight and dressing percentage, but significantly increased carcase dry matter, fat and energy content. 4. Adding BPM to diets had no effect on viscosity of diets and jejunal digesta, and minor effects on litter quality, whereas BPA increased the viscosity of diets and jejunal digesta, improved litter quality at 21 d, but decreased litter quality at 32 d. 5. To conclude, broiler chickens performed better on a BPM product with intact proteins than on an autolysate with ruptured cell walls and a high content of free amino acids and low molecular-weight peptides.  相似文献   

17.
试验通过分析饲粮不同赖氨酸水平对云岭红肉鸡生长性能、氮沉积率及血液生化指标的影响,确定云岭红肉鸡饲粮赖氨酸的适宜需要量。采用单因素试验设计,将180只健康状况良好、体重相近的1日龄云岭红肉鸡随机分为5个处理组,每组3个重复,每个重复12只鸡。5组试验鸡饲喂的饲粮赖氨酸水平分别为0.85%、0.95%、1.05%、1.15%、1.25%。结果表明:赖氨酸水平对试验鸡的期末体重、全期增重、平均日增重及料重比有显著影响(P<0.05),当赖氨酸水平为1.15%时期末体重最大739.21 g,全期增重最大701.66 g,平均日增重最大24.19 g,料重比最低1.68。不同赖氨酸水平对丝氨酸、甘氨酸和组氨酸的表观代谢率影响显著(P<0.05)。对血清总氨基酸和血清尿素氮影响显著(P<0.05)。研究表明,0~4周龄云岭红肉鸡适宜赖氨酸水平最适量为1.15%。  相似文献   

18.
试验采用2×5双因子完全随机试验设计,研究苏氨酸水平对1~14日龄北京鸭生产性能的影响,进而探讨1~14日龄北京鸭苏氨酸需要量。选取480只体重相近的1日龄健康的W系雄性北京鸭,随机分为10个处理,每个处理6个重复,每个重复8只北京鸭。试验日粮共设2个蛋白水平,分别为14%和20%;5个苏氨酸水平,分别为0.50%、0.58%、0.66%、0.74%、0.82%,各试验日粮的代谢能和其它必需氨基酸添加量一致。试验期为14d。研究结果表明:日粮蛋白质水平与苏氨酸水平产生明显的互作效应,且蛋白质水平与苏氨酸水平对1~14日龄北京鸭采食量、日增重、料重比影响均显著(P0.01)。在高蛋白质水平下,以采食量和日增重为评价指标,分别建立其与苏氨酸需要量的回归方程,得出均呈二次曲线变化,且1~14日龄北京鸭最佳苏氨酸需要量分别为0.688%、0.737%。在低蛋白质水平下,日增重和日采食量均随着苏氨酸水平的升高而升高,未出现下降或平台期,因此,低蛋白质水平下的苏氨酸需要量高于高蛋白质水平下的苏氨酸需要量,苏氨酸需要量与蛋白质有关。  相似文献   

19.
乳酸型复合酸化剂饲喂效果试验   总被引:3,自引:0,他引:3  
为了仔猪早期断奶,研制代乳品饲料,使仔猪正常生长,提高母猪生产能力成为畜牧生产中的关键。研究选用日龄(23~25日龄)、体重相近,公母对等,同批次断奶的杜长大三元杂交仔猪90头为研究对象,探讨乳酸型复合酸化剂饲喂效果。结果表明,在30 d的试验期,添加不同比例乳酸型复合酸化剂可极显著提高日增重15.31%(P<0.01)或27.25%(P<0.01);可显著提高日采食量6.65%(P<0.05)或8.27%(P<0.05);极显著降低耗料增重比9.24%(P<0.01)或15%(P<0.01),增重饲料成本显著降低7.07%(P<0.05)或极显著降低14.89%(P<0.01),在防病抗病和降低腹泻方面均有一定的效果。由研究结果来看乳酸型复合酸化剂具有推广价值。  相似文献   

20.
Two experiments were conducted to determine the effects of feed and water restriction and receiving diet crude protein level on feeder pig performance. In Exp. 1, a total of 239 commingled feeder pigs transported over 1,000 km were used in two trials. Pigs given access to feed and water (FW) at the market weighed more (P less than .0001) following marketing and transport than pigs given no feed and water (N) for the comparable 25-h market period (20.7 vs 19.6 kg). While FW pigs weighed less (P less than .02) than N pigs at the conclusion of the trials (93.9 vs 96.6 kg), there was no effect (P greater than .1) on overall average daily gain (.32 vs .35). There were no effects of receiving diets containing 12, 16 or 20% crude protein on daily gain, daily feed intake or feed efficiency for the overall growing-finishing period. Scour scores on d 8, 9, 10, 11, 13 and 14 post-arrival increased (P less than .01) with increased levels of protein in the receiving diets. In Exp. 2, a total of 360 crossbred feeder pigs was mixed and moved from a nursery to grower-finisher facilities in three trials, given feed and water access immediately (FWG) or denied access for 44 h (NG). At the end of the 44-h period, FWG pigs were heavier than NG pigs (P less than .0001; 18.3 vs 16.7 kg). There was no treatment effect on overall pig weight, daily gain or feed efficiency.  相似文献   

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