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1.
锐劲特对稻蝗的防治试验为了解锐劲特对稻蝗的防治效果,在荆门作了观察性试验。设每667m2锐劲特5ml、10ml,杀虫单50g,万灵10g,清水对照5个处理于7月24日蝗蝻5龄期作常规叶面喷雾,药前、药后1d、3d、7d、15d各调查1次10m2虫口密...  相似文献   

2.
稻瘟病菌对稻瘟灵抗性遗传研究   总被引:5,自引:0,他引:5  
在离体条件下就稻瘟病菌对稻瘟灵抗性的诱导、抗性水平和遗传进行了研究。结果表明,供试3个小种(ZA49、ZF和ZD)4个菌株分别经稻瘟灵50μg/ml、100μg/ml和稻瘟灵100μg/ml+亚硝基胍0.5μg/ml的3种处理诱变,均得到了抗稻瘟灵突变株,在含稻瘟灵的培养基中加入诱变剂亚硝基胍可显著提高稻瘟病菌对稻瘟灵抗性的突变率。上述3个不同处理获得的突变株的抗性水平相似,为野生型亲本的2.8~8.8倍。突变株对稻瘟灵的抗性在单分生孢子无性系后代可以稳定遗传。  相似文献   

3.
毒性基因缺失突变体Du728防治水稻白叶枯病的初步研究   总被引:4,自引:1,他引:3  
1995~1997年在温室和田间研究了稻白叶枯病菌毒性基因缺失突变体Du728对稻白叶枯病的防治效果,并对其作用机制进行了初步分析。温室试验结果表明:Du728失去了对供试水稻品种的致病性;在叶面喷雾一次Du728菌液(106cfu/ml)防病效果为48.5%,再增加菌量并不能显著提高防效;Du728与水杨酸(10μg/ml)混合喷雾处理的防治效果达到60%左右。Du728及其与水杨酸混合液喷雾处理后,最佳接种间隔期分别为1~2天和2天;防效持久期分别为7~10天和10~15天。田间小区试验表明,Du728与水杨酸混合使用(喷雾3次,间隔7~10天)比两者单独使用效果好,各处理小区产量增加14.0%~26.7%。田间自然发病情况下,Du728与水杨酸混合喷雾的防治效果(80.1%)比人工接种条件下的防效(54.4%)好,与叶枯灵处理的效果相当。从使用方法来看,喷雾或沾根后再喷雾的效果比只沾根的效果好。  相似文献   

4.
稻褐飞虱对杀虫剂的抗药性监测   总被引:1,自引:0,他引:1  
1994,1995年用点滴法和药膜法,分别测定了监利县褐飞虱对甲胺磷,扑虱灵的抗性水平,结果表明,对甲胺磷的LD50两年分别为0.0166,0.0286μg/头,以郴州的LD50为敏感基线标准比较抗生倍数为1.5,2.7,扑虱灵的LC50两年分别为0.001523,0.002969mg/ml,以玉林的LC50为敏感基线标准比较。  相似文献   

5.
作者采用饲料混药法测定了齐墩螨素对不同龄期棉铃虫的毒力。结果表明,处理后48h和96h对初孵、1龄、3龄、4龄幼虫的致死中浓度LC50分别为0.0141、0.049、0.58、1.14和0.005、0.021、0.64、1.13μg/g,同时还观察到1龄幼虫经处理96h后幼虫体长抑制率与药剂浓度成正相关,当药剂浓度为0.00625、0.0125、0.025和0.05μg/g时,体长抑制率分别为9.  相似文献   

6.
农抗120中组份A‘对几种作物病原真菌抑菌活性的研究   总被引:1,自引:0,他引:1  
本文测定了农抗120中组份A'对水稻纹枯菌、棉花立枯病菌、棉花枯萎菌和苹果树腐烂病菌的抑菌活性。结果表明:农抗120A'对水稻纹枯病菌和棉花立枯病菌有强烈的抑制作用,其最低抑菌浓度均为0.25μg/mL,24小时抑菌率达50%的抑菌浓度分别为1.0μg/mL和2.5μg/mL、且抑菌强度与120A'浓度成比。  相似文献   

7.
试验表明,水稻播种时用中生菌素60倍55~60℃药液浸种,任其自然冷却48h;稻苗3~4叶期和移栽前5~7d分别用中生菌素200倍液喷雾1次,可推迟田间白叶枯病的发病始期20d以上,并显著降低其发病程度,对于轻病田或轻发生年份,大田可免于防治,重发生年份在8月上旬再用中生菌素200倍液喷雾1次,可更有效地控制病情的发展。  相似文献   

8.
水杨酸对水稻幼苗抗瘟性的诱导作用   总被引:51,自引:3,他引:51  
 网室试验证明,水杨酸(SA)叶面喷雾可减轻水稻幼苗稻瘟病的发生。在平板培养中,SA对稻瘟菌分生孢子的萌发及菌丝生长均无明显抑制作用,因此认为SA处理后稻瘟病病情指数的下降是由于SA处理提高了水稻幼苗的抗瘟性,即稻苗产生了诱导抗性而引起的。SA诱发水稻幼苗产生对苗叶瘟诱导抗性的浓度为0.01~0.1mM。SA喷雾处理后2~4天接种,诱导抗性表现最强。0.01mMSA在接种前2天喷雾处理三叶一心期稻苗一次后,水稻叶片的病斑数目和大小比清水对照分别降低72.7%和55.4%,病指比对照降低59.8%,抗瘟性的持久期为15天(病指降低24.3%)。经0.01mMSA诱导处理后再用同浓度SA进行一次强化处理可增强诱抗效果,延长抗性持久期。用0.01mMSA处理水稻第一和第二叶可使未经处理的第三和第四叶上产生系统性抗瘟性。  相似文献   

9.
本研究以多年筛选出的东北霜霉菌Peronosporamanschurica(Naum.)Syd.为诱导菌,在黄瓜植株的3~4叶期,采用茎部注射、喷雾和灌根等三种诱导接种方法,诱导植株产生诱导抗性,测定东北霜霉菌对黄瓜霜霉病的免疫作用。研究结果表明:东北霜霉菌免疫作用的强弱与诱导接种方法有关。在3种诱导接种方法中,以注茎诱导接种法的免疫作用最强,使黄瓜植株产生的系统保护作用最明显,表现为黄瓜霜霉病的发病始期较对照株向后推迟12~13天,平均病情指数降低17.6;喷雾诱导接种的免疫作用较弱;而灌根诱导接种法,未能反应出东北霜霉菌的免疫作用  相似文献   

10.
小麦纹枯病药剂防治技术   总被引:7,自引:0,他引:7  
进行了防治小麦纹枯病单剂和混剂农药的筛选及其应用技术的研究。结果表明,供试药剂对小麦纹枯病菌都具有抑制作用,但其有效浓度有所不同。福美双,多菌,灵,三唑酮,担菌灵和井冈霉素的EC50分别为12.2857、4.0346、1.7824、4.0346和16.9200μg/ml;混剂福美双十三唑酮的EC50为0.28050μg/ml,共毒系为1109.857,具有明显增效作用。在此基础上,田间比较了上述药  相似文献   

11.
Fifteen species of dermestid beetles were recorded at ‘Evolution Canyon’ (EC), Lower Nahal Oren, Mt. Carmel, Israel. They represent ~35% of known Israeli dermestid species. The following three species were recorded for the first time in Israel:Trogoderma svriaca Dalla Torre, 1911;Ctesias svriaca Ganglbauer, 1904; andAnthrenus (s.str.) jordaniens Pic, 1934. Adults of 13 species were collected on the more solar radiated, warmer and climatically more fluctuating south-facing slope (SFS); ten species were collected on the opposite, north-facing slope (NFS), which was cooler and climatically more stable. The abundance of adult dermestid beetles was 1.9 times higher on the SFS than on the NFS (86 and 47, respectively). Species richness and abundance distribution at EC (three collecting stations on each slope and one at the valley bottom) were significantly negatively correlated with the plant cover that consisted of trees and bushes (Spearmanr s ,P=0.007 and 0.039, respectively) and perennials (Spearmanr s ,P=0.039 and 0.077, respectively), indicating that non-woody plants were preferred by adult dermestid beetles.  相似文献   

12.
Molecular diagnostic techniques have been developed to differentiate the Ascochyta pathogens that infect cool season food and feed legumes, as well as to improve the sensitivity of detecting latent infection in plant tissues. A seed sampling technique was developed to detect a 1% level of infection by Ascochyta rabiei in commercial chickpea seed. The Ascochyta pathogens were shown to be genetically diverse in countries where the pathogen and host have coexisted for a long time. However, where the pathogen was recently introduced, such as A. rabiei to Australia, the level of diversity remained relatively low, even as the pathogen spread to all chickpea-growing areas. Pathogenic variability of A. rabiei and Ascochyta pinodes pathogens in chickpea and field pea respectively, appears to be quantitative, where measures of disease severity were based on aggressiveness (quantitative level of infection) rather than on true qualitative virulence. In contrast, qualitative differences in pathogenicity in lentil and faba bean genotypes indicated the existence of pathotypes of Ascochyta lentis and Ascochyta fabae. Therefore, reports of pathotype discrimination based on quantitative differences in pathogenicity in a set of specific genotypes is questionable for several of the ascochyta-legume pathosystems such as A. rabiei and A. pinodes. This is not surprising since host resistance to these pathogens has been reported to be mainly quantitative, making it difficult for the pathogen to overcome specific resistance genes and form pathotypes. For robust pathogenicity assessment, there needs to be consistency in selection of differential host genotypes, screening conditions and disease evaluation techniques for each of the Ascochyta sp. in legume-growing countries throughout the world. Nevertheless, knowledge of pathotype diversity and aggressiveness within populations is important in the selection of resistant genotypes.  相似文献   

13.
Recent data on the epidemiology of the common mycotoxigenic species of Fusarium, Alternaria, Aspergillus and Penicillium in infected or colonized plants, and in stored or processed plant products from the Mediterranean area are reviewed. Emphasis is placed on the toxigenicity of the causal fungal species and the natural occurrence of well known mycotoxins (aflatoxins, ochratoxins, fumonisins, trichothecenes, zearalenone, patulin, Alternaria-toxins and moniliformin), as well as some more recently described compounds (fusaproliferin, beauvericin) whose toxigenic potential is not yet well understood. Several Fusarium species reported from throughout the Mediterranean area are responsible of the formation of mycotoxins in infected plants and in plant products, including: Fusarium graminearum, F. culmorum, F. cerealis, F. avenaceum, F. sporotrichioides and F. poae, which produce deoxynivalenol, nivalenol, fusarenone, zearalenone, moniliformin, and T-2 toxin derivatives in wheat and other small grains affected by head blight or scab, and in maize affected by red ear rot. Moreover, strains of F. verticillioides, F. proliferatum, and F. subglutinans, that form fumonisins, beauvericin, fusaproliferin, and moniliformin, are commonly associated with maize affected by ear rot. Fumonisins, were also associated with Fusarium crown and root rot of asparagus and Fusarium endosepsis of figs, caused primarily by F. proliferatum. Toxigenic A. alternata strains and associated tenuazonic acid and alternariols were commonly found in black mould of tomato, black rot of olive and citrus, black point of small cereals, and black mould of several vegetables. Toxigenic strains of A. carbonarius and ochratoxin A were often found associated with black rot of grapes, whereas toxigenic strains of A. flavus and/or P. verrucosum, forming aflatoxins and ochratoxin A, respectively, were found in moulded plant products from small cereals, peanuts, figs, pea, oilseed rape, sunflower seeds, sesame seeds, pistachios, and almonds. Finally, toxigenic strains of P. expansum and patulin were frequently found in apple, pear and other fresh fruits affected by blue mould rot, as well as in derived juices and jams.  相似文献   

14.
We first discuss the diversity of fruit fly (Diptera: Tephritidae) parasitoids (Hymenoptera) of the Neotropics. Even though the emphasis is on Anastrepha parasitoids, we also review all the information available on parasitoids attacking flies in the genera Ceratitis, Rhagoletis, Rhagoletotrypeta, Toxotrypana and Zonosemata. We center our analysis in parasitoid guilds, parasitoid assemblage size and fly host profiles. We also discuss distribution patterns and the taxonomic status of all known Anastrepha parasitoids. We follow by providing a historical overview of biological control of pestiferous tephritids in Latin American and Florida (U.S.A.) and by analyzing the success or failure of classical and augmentative biological control programs implemented to date in these regions. We also discuss the lack of success of introductions of exotic fruit fly parasitoids in various Latin American countries. We finish by discussing the most pressing needs related to fruit fly biological control (classical, augmentative, and conservation modalities) in areas of the Neotropics where fruit fly populations severely restrict the development of commercial fruit growing. We also address the need for much more intensive research on the bioecology of native fruit fly parasitoids.  相似文献   

15.
The genera ofMicrogaster Latreille 1804 andHygroplitis Thomson 1895 from China are presented systematically in this paper. Thirty-two species ofMicrogaster and three species ofHygroplitis are known in China. Diagnosis, character variation, distribution and host of each species among the two genera are presented, including its host and distribution. Keys to the species ofMicrogaster andHygroplitis are given. http://www.phytoparasitica.org posting Dec. 19, 2006.  相似文献   

16.
Plant Viruses Transmitted by Whiteflies   总被引:18,自引:0,他引:18  
One-hundred and fourteen virus species are transmitted by whiteflies (family Aleyrodidae). Bemisia tabaci transmits 111 of these species while Trialeurodes vaporariorum and T. abutilonia transmit three species each. B. tabaci and T. vaporariorum are present in the European–Mediterranean region, though the former is restricted in its distribution. Of the whitefly-transmitted virus species, 90% belong to the Begomovirus genus, 6% to the Crinivirus genus and the remaining 4% are in the Closterovirus, Ipomovirus or Carlavirus genera. Other named, whitefly-transmitted viruses that have not yet been ranked as species are also documented. The names, abbreviations and synonyms of the whitefly-transmitted viruses are presented in tabulated form together with details of their whitefly vectors, natural hosts and distribution. Entries are also annotated with references. Whitefly-transmitted viruses affecting plants in the European–Mediterranean region have been highlighted in the text.  相似文献   

17.
《干旱区科学》2014,(6):782-782
正Journal of Arid Land(JAL)is an international journal(ISSN 1674-6767;CN 65-1278/K)for the natural sciences,sponsored by the Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences and Science Press.It is published by Science Press and Springer-Verlag Berlin Heidelberg bimonthly.JAL publishes original,innovative,and integrative research from arid and semiarid regions,ad  相似文献   

18.
Solacol®, a formulation of the antibiotic validamycin, at 0.33% in 2% malt extract agar, reduced the spread of fungi on dilution plates drastically and allowed twice as much incubation time before subculturing; this resulted in an elevated number of species isolated. Using pure cultures of 62 common soil fungi, it was shown that all fast-growing species (exceptPythium ultimum) were efficiently inhibited but not completely suppressed. Inhibition was comparable to that by 0.5% oxgall, though, while this substance completely suppressed several species, Solacol very strongly inhibited onlyGaeumannomyces graminis, Gerlachia nivalis, Harzia acremonioides, Verticillium biguttatum andRhizoctonia solani. In a further experiment each separate constituent of Solacol was tested against 22 fungi at equivalent concentrations. Validamycin strongly inhibitedChaetomium globosum and two Basidiomycetes, though hardly more than the non-ionic detergent which mainly inhibited the other fungi. A few species were, however, more inhibited by Solacol than by the detergent alone. Solacol at 0.33% is a suitable aid in dilution plating of soil fungi, by increasing the number of colonies and species observed.Samenvatting Solacol®, een formulering van het antibioticum validamycine, remde de groei van schimmels in verdunningsplaten met een concentratie van 0.33% in 2% moutagar en maakte het mogelijk de periode tot afenten met een factor 2 te verlengen; daardoor was het aantal geïsoleerde soorten duidelijk toegenomen. Met reincultures van 62 algemene grondschimmelsoorten werd aangetoond, dat alle snelgroeiende soorten (met uitzondering vanPythium ultimum) voldoende geremd, maar niet volkomen onderdrukt werden. Het remmingspercentage was vergelijkbaar met dat van 0.5% ossegal, hoewel dit laatste sommige soorten volkomen onderdrukte; Solacol remde alleenGaeumannomyces graminis, Gerlachia nivalis, Harzia acremonioides, Verticillium biguttatum enRhizoctonia solani zeer sterk. In een volgend experiment werden de componenten van Solacol t.o.v. 22 fungi apart getoetst in concentraties equivalent aan 0.33% Solacol. Validamycine remde alleenChaetomium globosum en twee basidiomyceten behoorlijk, maar nauwelijks meer dan de niet-ionische uitvloeier, die in hoofdzaak de overige remeffecten veroorzaakte. Enkele soorten werden echter door het complete Solacol veel sterker geremd dan door de uitvloeier alleen. Solacol in een verdunning van 0,33% wordt aanbevolen bij verdunningsplaten voor het isoleren van grondschimmels ten einde het aantal kolonies en soorten te verhogen.  相似文献   

19.
Broad bean mottle virus (BBMV) was transmitted from infected to healthy faba-bean plants by the curculionid weevilsApion radiolus Kirby,Hypera variabilis Herbst,Pachytychius strumarius Gyll,Smicronyx cyaneus Gyll, andSitona lineatus L. The latter appeared to be an efficient vector: acquisition and inoculation occurred at the first bite, the rate of transmission was c. 41%, and virus retention lasted for at least seven days.S. lineatus transmitted the virus from faba bean to lentil and pea, but not to the three genotypes of chickpea tested. This is the first report on the generaHypera, Pachytychius, andSmicronyx as virus vectors, and onA. radiolus, H. variabilis, P. strumarius, andS. cyaneus as vectors of BBMV.Out of 351 samples of food legumes with symptoms suggestive of virus infection, 16, 11, 19, and 17% of the samples of chickpea, lentil, pea, and common bean, respectively, were found infected when tested for BBMV in DAS-ELISA. This is the first report on the natural occurrence of BBMV in chickpea, lentil, pea, and common bean. The virus should be regarded as a food-legume virus rather than a faba-bean virus solely, and is considered an actual threat to food legume improvement programmes.  相似文献   

20.
A collection of 38 PVY isolates from seed potato batches, originating from several Western European countries, was characterized by using current biological, serological and molecular tools differentiating PVY strains and groups. The correlation between the three kinds of tests was good but not absolute. No single serological or PCR method was able to discriminate among the five isolate groups found. Twenty-nine isolates belonged to the PVYN strain and six to the PVYO strain. No PVYC was found. Two other isolates reacted serologically like PVYO, but were unable to elicit a hypersensitive response from the Nytbr gene and probably represent the PVYZ group. At the molecular level, these two isolates showed a combination of both PVYO and PVYN and could be recombinants of these strains. Another isolate reacted serologically like PVYO, but induced vein necrosis in tobacco, like PVYN-Wilga. Some PVYN isolates caused tuber ring necrosis in glasshouse conditions. These might belong to the PVYNTN group. The PVYNTN, PVYN-Wilga and PVYZ groups probably represent pathotypes within strains PVYN and PVYO, respectively. The present study also confirms previous reports showing a high genetic variation at the 5 end within the PVYN strain.  相似文献   

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