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1.
Kamlesh Jangid Mark A. Williams Thomas M. Schmidt William B. Whitman 《Soil biology & biochemistry》2011,43(10):2184-2193
The response of soil microbial communities following changes in land-use is governed by multiple factors. The objectives of this study were to investigate (i) whether soil microbial communities track the changes in aboveground vegetation during succession; and (ii) whether microbial communities return to their native state over time. Two successional gradients with different vegetation were studied at the W. K. Kellogg Biological Station, Michigan. The first gradient comprised a conventionally tilled cropland (CT), mid-succession forest (SF) abandoned from cultivation prior to 1951, and native deciduous forest (DF). The second gradient comprised the CT cropland, early-succession grassland (ES) restored in 1989, and long-term mowed grassland (MG). With succession, the total microbial PLFAs and soil microbial biomass C consistently increased in both gradients. While bacterial rRNA gene diversity remained unchanged, the abundance and composition of many bacterial phyla changed significantly. Moreover, microbial communities in the relatively pristine DF and MG soils were very similar despite major differences in soil properties and vegetation. After >50 years of succession, and despite different vegetation, microbial communities in SF were more similar to those in mature DF than in CT. In contrast, even after 17 years of succession, microbial communities in ES were more similar to CT than endpoint MG despite very different vegetation between CT and ES. This result suggested a lasting impact of cultivation history on the soil microbial community. With conversion of deciduous to conifer forest (CF), there was a significant change in multiple soil properties that correlated with changes in microbial biomass, rRNA gene diversity and community composition. In conclusion, history of land-use was a stronger determinant of the composition of microbial communities than vegetation and soil properties. Further, microbial communities in disturbed soils apparently return to their native state with time. 相似文献
2.
Rima Upchurch Chih-Yu Chiu Karin Everett Glen Dyszynski David C Coleman William B Whitman 《Soil biology & biochemistry》2008,40(6):1294-1305
Differences in the bacterial communities of soils caused by disturbances and land management were identified in rRNA gene libraries prepared from conventional tilled (CT) and no tilled (NT) cropland, a successional forest after 30 y of regrowth (NF) and an old forest of >65 y (OF) at Horseshoe Bend, in the southern Piedmont of Georgia (USA). Libraries were also prepared from forests after 80 y of regrowth at the Coweeta Long Term Ecological Research site (CWT) in the Southern Appalachians of western North Carolina (USA). The composition of the bacterial communities in cropland soils differed from those of the Horseshoe Bend OF and CWT forest soils, and many of the most abundant OTUs were different. Likewise, the diversity of bacterial communities from forest was less than that from cropland. The lower diversity in forest soils was attributed to the presence of a few, very abundant taxa in forest soils that were of reduced abundance or absent in cropland soils. After 30 y of regrowth, the composition of the bacterial soil community of the NF was similar to that of the OF, but the diversity was greater. These results suggested that the bacterial community of soil changes slowly within the time scale of these studies. In contrast, the composition and diversity of the bacterial communities in the Horseshoe Bend OF and Coweeta soils were very similar. Thus, this forest soil bacterial community was widely distributed in spite of the differences in soil properties, vegetation, and climate as well as resilient to disturbances of the above ground vegetation. 相似文献
3.
川西北不同沙化程度草地土壤细菌群落特征 总被引：2，自引：1，他引：2
研究川西北不同沙化程度草地（未沙化草地、轻度沙化草地、中度沙化草地、重度沙化草地）土壤细菌多样性和群落结构特征,利用Illumina二代高通量测序技术MiSeq对土壤细菌的16 SrRNA V3—V4可变区进行测序,研究土壤细菌多样性、物种组成和丰富度,并结合土壤理养分探讨影响细菌群落结构的环境因素,对发挥土壤潜在肥力,了解土壤健康状况,实现该区植被的管理与可持续利用有着重要的意义。结果表明:（1）不同沙化草地土壤养分具有明显差异,依次表现为:随着沙化程度的增加,土壤pH值逐渐增加,而土壤有机碳、全氮、全钾、碱解氮和速效磷逐渐降低;（2）不同沙化程度草地土壤样品中共检测到细菌的32个门,65个纲,169个目,优势菌门为变形菌门（Proteobacteria）、放线菌门（Actinobacteria）、酸杆菌门（Acidobacteria）、绿弯菌（Chloroflexi）、浮霉菌门（Planctomycetes）,主要的优势菌纲为放线杆菌纲（Actinobacteria）、α-变形菌纲（α-Proteobacteria）、酸杆菌纲（Acidobacteria）、β-变形菌纲（β-Proteobacteria）、浮霉菌纲（Planctomycetacia）,与沙化草地相比,未沙化草地优势菌主要是变形菌门（Proteobacteria）和放线菌门（Actinobacteria）;（3）随着沙化程度的增加,OUT数目、Chao指数、Ace指数、Shannon指数逐渐减小,其中不同沙化草地土壤细菌覆盖率和Simpson指数差异不显著（p > 0.05）;（4）冗余分析和Pearson相关性分析表明,土壤pH值、土壤有机碳（SOC）和全氮（TN）是土壤细菌群落结构和多样性的主要影响因子。 相似文献
4.
Chenxu Liu Qimei Lin Guitong Li 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(7):618-630
ABSTRACT Our objectives were to investigate whether AP affects the soil bacterial community composition and diversity in high-level available phosphorus (AP) soils. The bacterial community was analysed through high-throughput sequencing using the Illumina MiSeq platform. Fifteen soils, including barren land, cropland and greenhouse soils which were sandy loam Fluvisols, were selected from different fields in Beijing, China, with AP contents ranging from 5.03 to 391.45 mg kg?1. Statistical analyses revealed high AP (>100 mg kg?1) decreased alpha diversity (Shannon’s index, H’) but not beta diversity of the soil bacterial community. The sequencing of 16S rRNA genes showed that Proteobacteria, Bacteroidetes and Acidobacteria were the dominant phyla in sandy loam Fluvisols. AP, soil organic carbon (SOC) and total nitrogen (TN) had synergistic influence on the shift of the bacterial community composition. Moreover, AP was the main driving factor affecting the soil bacterial community composition compared with other environmental factors. The members of the Proteobacteria, Bacteroidetes and Actinobacteria belonging to copiotrophic taxa typically increased in relative abundance in high-P soils, while oligotrophic taxa (mainly Acidobacteria) decreased in relative abundance. Our results demonstrated that the bacterial community composition would shift from oligotrophic to copiotrophic with increasing levels of AP. 相似文献
5.
The survey presented here describes the bacterial diversity and community structures of a pristine forest soil and an anthropogenic terra preta from the Western Amazon forest using molecular methods to identify the predominant phylogenetic groups. Bacterial community similarities and species diversity in the two soils were compared using oligonucleotide fingerprint grouping of 16S rRNA gene sequences for 1500 clones (OFRG) and by DNA sequencing. The results showed that both soils had similar bacterial community compositions over a range of phylogenetic distances, among which Acidobacteria were predominant, but that terra preta supported approximately 25% greater species richness. The survey provides the first detailed analysis of the composition and structure of bacterial communities from terra preta anthrosols using noncultured-based molecular methods. 相似文献
6.
The elevational patterns of diversity for plants and animals have been well established over the past century. However, it is unclear whether there is a general elevational distribution pattern for microbes. Changbai Mountain is one of few well conserved natural ecosystems, where the vertical distribution of vegetation is known to mirror the vegetation horizontal zonation from temperate to frigid zones on the Eurasian continent. Here, we present a comprehensive analysis of soil bacterial community composition and diversity along six elevations representing six typical vegetation types from forest to alpine tundra using a bar-coded pyrosequencing technique. The bacterial communities differed dramatically along elevations (vegetation types), and the community composition was significantly correlated with soil pH, carbon/nitrogen ratio (C/N), moisture or total organic carbon (TOC), respectively. Phylogenetic diversity was positively correlated with soil pH (P = 0.024), while phylotype richness was positively correlated with soil pH (P = 0.004), total nitrogen (TN) (P = 0.030), and negatively correlated with C/N ratio (P = 0.021). Our results emphasize that pH is a better predictor of soil bacterial elevational distribution and also suggest that vegetation types may indirectly affect soil bacterial elevational distribution through altering soil C and N status. 相似文献
7.
The influence of soil properties on the structure of bacterial and fungal communities across land-use types 总被引：3，自引：1，他引：3
Christian L. Lauber Michael S. Strickland Mark A. Bradford Noah Fierer 《Soil biology & biochemistry》2008,40(9):2407
Land-use change can have significant impacts on soil conditions and microbial communities are likely to respond to these changes. However, such responses are poorly characterized as few studies have examined how specific changes in edaphic characteristics do, or do not, influence the composition of soil bacterial and fungal communities across land-use types. Soil samples were collected from four replicated (n = 3) land-use types (hardwood and pine forests, cultivated and livestock pasture lands) in the southeastern US to assess the effects of land-use change on microbial community structure and distribution. We used quantitative PCR to estimate bacterial–fungal ratios and clone libraries targeting small-subunit rRNA genes to independently characterize the bacterial and fungal communities. Although some soil properties (soil texture and nutrient status) did significantly differ across land-use types, other edaphic factors (e.g., pH) did not vary consistently with land-use. Bacterial–fungal ratios were not significantly different across the land-uses and distinct land-use types did not necessarily harbor distinct soil fungal or bacterial communities. Rather, the composition of bacterial and fungal communities was most strongly correlated with specific soil properties. Soil pH was the best predictor of bacterial community composition across this landscape while fungal community composition was most closely associated with changes in soil nutrient status. Together these results suggest that specific changes in edaphic properties, not necessarily land-use type itself, may best predict shifts in microbial community composition across a given landscape. In addition, our results demonstrate the utility of using sequence-based approaches to concurrently analyze bacterial and fungal communities as such analyses provide detailed phylogenetic information on individual communities and permit the robust assessment of the biogeographical patterns exhibited by soil microbial communities. 相似文献
8.
Minna Wu Hongling Qin Zhe Chen Jinshui Wu Wenxue Wei 《Biology and Fertility of Soils》2011,47(4):397-405
We investigated the effect of long-term fertilization on bacterial abundance, composition, and diversity in paddy soil. The
experiment started in 1990 in Taoyuan Agro-ecosystem Research Station in China (111°33′ E, 28°55′ N). The molecular approaches
including real-time quantitative PCR, terminal restriction fragment length polymorphism, and clone library construction were
employed using 16S rRNA gene as genetic marker. Application of inorganic fertilizers did not affect bacterial abundance, and
rice straw incorporation combined with inorganic fertilizers significantly (P < 0.05) increased bacterial abundance with shifts in bacterial community composition. Among phylogenetic groups, γ-Proteobacteria was responsive to all fertilization regimes while Acidobacteria was relatively stable to fertilization practices.
Inorganic fertilizer mainly affected γ-Proteobacteria and δ-Proteobacteria, while rice straw incorporation influenced β-Proteobacteria
and Verrucomicrobia. Therefore, long-term fertilization can affect abundance and composition of bacterial communities in paddy
soil. 相似文献
9.
The ecological staircase of Mendocino (California, USA) is characterized by a succession of uplifted marine terraces that are derived from the same mineralogical parent material but have different ages, levels of fertility, and types of vegetation, from grassland in the youngest and most fertile terrace to a pygmy forest in the older terraces. Such conditions present a unique opportunity to determine how the structure, abundance, and function of bacterial communities vary with soil fertility along this natural chronosequence. Pyrosequencing analysis of 16S rRNA gene amplicons revealed that Acidobacteria, Proteobacteria, Actinobacteria, and Bacteroidetes were the most abundantly represented phyla. Bacteroidetes, Firmicutes, Verrucomicrobia were significantly enriched in the grasslands, while the less fertile forested terraces showed higher abundance of Acidobacteria Gp2 and Alphaproteobacteria. The pygmy forest soil harboured significantly more Actinobacteria and OP10 than the non-pygmy forest. Between samples from different terraces, the structure of the bacterial community clearly correlated with soil characteristics. Notably, the number of operational taxonomic units was greater in the fertile terrace, as was the density of culturable bacterial populations. Functional characterization of the soil culturable bacteria from the pygmy and non-pygmy forest terraces revealed that the soil bacteria from the non-pygmy terrace were significantly more effective in solubilizing minerals and more abundant than in the pygmy terrace. Our results provide new information on bacterial community structure as a function of soil age, land cover and fertility, which improve our understanding of soil evolution. 相似文献
10.
Bacterial communities living in forest soils contribute to the decomposition of organic matter and the recycling of nutrients in these ecosystems and form one of the most diverse habitats on Earth. Unfortunately, due to difficulty in culturing soil bacteria, the understanding of their ecology is still limited. In the case of temperate deciduous forests, soil microbial communities face large seasonal variations in environmental conditions, such as temperature or moisture. Moreover, the supply of nutrients also differs due to seasonal processes, such as the allocation of photosynthates into soil by the roots of primary producers or the seasonal input of fresh litter. The aim of this study was to reveal how the bacterial community responds to these seasonal processes in the litter and soil of a Quercus petraea forest. Bacterial communities from litter and from the organic and mineral horizons of soil were analyzed during the four seasons of the year by 16S rRNA gene pyrosequencing. The results revealed that the composition of the bacterial community is horizon specific. The litter horizon had a higher relative abundance of Proteobacteria and Bacteroidetes than soil, while the organic and mineral horizons had a higher abundance of Acidobacteria, Firmicutes and Actinobacteria than litter. Moreover, the bacterial community was significantly affected by seasonality in all horizons. Bacterial communities in the litter showed significant differences between the vegetation season (May and July) and the autumn and winter seasons (October, February). In mineral soil, bacterial community composition was specific in the summer, when it was significantly different from all other seasons, with a larger number of taxa described as rhizosphere and mycorrhizosphere inhabitants. The results indicate that litter decomposition is the main driver of bacterial community composition in litter horizon. In contrast to reports on fungal communities, bacterial community composition in mineral soil responds to the seasonal peaks of rhizodeposition in the summer. 相似文献
11.
为探明连续马铃薯、玉米单作及间作种植对土壤细菌群落组成的影响,利用IonS5~(TM)XL高通量测序平台,分析了单作玉米(M)、单作马铃薯(P)、马铃薯||玉米间作(PM)下,土壤细菌群落组成以及多样性间的差异。结果表明:与单作相比,马铃薯||玉米间作土壤有机质含量显著升高(P0.05),但土壤全氮、碱解氮、全磷、速效钾、土壤pH等没有显著变化。所获得的56 787个土壤细菌可操作分类单元(OTUs)共分为46门、55纲、114目、208科、455属。土壤变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)和放线菌门(Actinobacteria)细菌占总相对丰度的57.68%～65.11%,为优势菌门;间作对土壤细菌群落多样性(香农指数、辛普森指数)、丰富度(ACE指数和Chao1指数)无显著影响,但改变了基于门、属水平上的细菌群落组成。与单作马铃薯相比,间作显著降低了土壤变形菌门(Proteobacteria)相对丰度(P=0.023),提高了浮霉菌门(Planctomycetes)的相对丰度(P=0.043)。在属水平上,相对丰度较低的芽单胞菌属(Gemmatimonas)、Candidatus Solibacter属更易受到种植方式的影响;间作提高了节杆菌属(Arthrobacter)、芽球菌属(Blastococcus)和芽孢杆菌属(Bacillus)的相对丰度。随细菌群落结构变化,细菌群落功能上出现差异,通过KEGG功能预测共得到7个一级功能层, 35个二级功能层,表现出功能上的丰富性,土壤细菌群落在代谢、遗传信息处理和细胞过程方面功能活跃。7个一级功能层中的代谢功能组在马铃薯||玉米间作与马铃薯单作间有显著差异。利用前向选择,经蒙特卡罗检验表明,连续马铃薯、玉米单作及间作栽培5年后的土壤各理化性状指标与土壤细菌群落组成、多样性间的相关性均不显著。连续马铃薯||玉米间作及单作5年条件下土壤细菌群落组成的变化是由马铃薯||玉米间作作物种间互利和竞争关系而驱动的。 相似文献
12.
Land-use conversion affects the soil community and microbial abundance, which are essential dynamic indicators of soil quality and sustainability. However, little to no work has been performed to analyse the impact from different land-use histories (i.e. fallow, tea, rice, banana, and maize) on the microbial abundance and diversity in the soil of sacha inchi (Plukenetia volubilis L.). Real-time quantitative PCR (qPCR) was performed to quantify soil bacterial and fungal abundance. Denaturing gradient gel electrophoresis (DGGE) combined with cloning and sequencing was used to assess the microbial communities. Our results showed that the bacterial and fungal abundance in fallow land-use conversion soils was significantly lower than that in the other four land-use conversion soils (tea, rice, banana, and maize). Moreover, the highest abundance of bacteria and fungi was detected in the soils converted from maize to sacha inchicultivation. In addition, canonical correspondence analysis (CCA) showed that the total N and pH were significantly related to bacterial and fungal community structures. These results suggest that land-use conversion from maize fields to sacha inchi farms is an effective way to maintain the soil microbial quantity and hence the sustainability of the soil. 相似文献
13.
Purpose
The bacterial phylum Verrucomicrobia plays important roles in biogeochemical cycling processes, while the ecology of this phylum is still unclear. Previous elevational studies mainly focused on whole bacterial communities, while no study exclusively picked out Verrucomicrobia. Our objectives were to investigate the abundance, diversity and community composition of soil Verrucomicrobia across an elevation gradient on Changbai Mountain.Materials and methods
In total, 24 soil samples representing six elevation gradients were collected. Primer set 515F/806R was used for PCR amplifications and sequencing was conducted on an Illumina HiSeq2000 platform. Data sets comprising of Verrucomicrobial phylum were culled from all quality sequences for the further analyses of Verrucomicrobial diversity and community composition.Results and discussion
The relative abundance of Verrucomicrobia accounted for ~20% of the total bacterial communities, and Spartobacteria and DA101 were the most dominant class and genus, respectively. Verrucomicrobia community composition differed significantly among elevations, while the Verrucomicrobia diversity showed no apparent trend along elevation although the richness of some classes or genera significantly changed with elevation. The Verrucomicrobial community composition, diversity, and relative abundance of specific classes or genera were significantly correlated with soil pH and carbon/nitrogen ratio (C:N ratio).Conclusions
These results indicated that Verrucomicrobia were abundant in Changbai Mountain soils, and Verrucomicrobial elevational distribution was strongly influenced by soil pH and C:N ratio. Our results also provide potential evidence that the dominant genus DA101 occupies different ecological niches and performs oligotrophic life history strategy in soil environment.14.
复垦土壤细菌群落结构及其与土壤肥力的关系 总被引：1，自引：1，他引：0
为了解两淮煤矿区复垦状况及其对土壤微生物的影响机制,合理人工干预,快速有效提高复垦土壤生产力,本研究以煤矸石充填复垦土壤为研究对象,通过野外调查与采样分析,采用Illumina MiSeq 高通量测序分析土壤细菌特定基因片段V4 区域,基于非度量多维尺度分析、冗余分析、方差分析、肥力指数、回归模型方法,对矸石充填土壤不同复垦方向的土壤细菌优势群落和生物多样性进行了探索,明晰土壤细菌群落及其与土壤肥力的响应作用。研究结果表明：细菌群落组成上,不同复垦方向对细菌优势群落分布并无显著性影响,变形菌门、酸杆菌门和放线菌门菌群为主要优势菌群。土壤微生物多样性层面上,表层土壤微生物有较高的丰富度和多样性,均匀度并无显著性差异。微生物多样性在不同复垦方向不存在显著性差异,Shannon_Wiener指数介于5.23～6.91之间,Chao1指数为867.1～5436,Pielou指数介于0.79～0.84之间,表中层同深层土壤的细菌群落结构具有差异性。TN、SOM、AP和AK是影响土壤细菌群落的主要肥力因子,土壤肥力质量指数与奇古菌门相对丰度呈显著正相关（P＜0.01）,同链霉菌属呈显著负相关（P＜0.05）,在一定程度上可将二者的相对丰度作为评价复垦土壤肥力状况的重要生物指标。研究成果可为两淮矿区矸石充填复垦土壤在微生物层面提高肥力质量提供理论支持。 相似文献
15.
黑河中游不同土地利用方式地面节肢动物对土壤盐渍化的响应 总被引：1，自引：0，他引：1
在甘肃河西走廊黑河中游荒漠绿洲过渡区,天然沙质草地被相继转变为农田和防风固沙人工林,但目前尚缺乏不同土地利用/管理方式下地面节肢动物群落对土壤盐渍化响应的系统研究。以天然沙质草地转变的人工梭梭灌木林、人工杨树林、人工樟子松林和农田为研究对象,以天然草地为对照,基于5种研究样地表层土壤盐分及其组成和地面节肢动物群落的观测数据,采用RDA(Redundancy analysis)排序分析等方法,研究了不同土地利用/管理方式下地面节肢动物个体数量和类群丰富度对土壤盐分环境变化的响应机制。主要结果为:(1)土地利用变化与管理措施相互作用驱动了地面节肢动物群落的演变过程,天然草地植被转变为人工林和农田20多年后,显著降低了地面节肢动物群落的数量而对类群丰富度无显著影响。(2)地面节肢动物群落的变化受土壤pH、Na+、Mg2+、Cl-离子的共同影响,其中土壤pH、Na+和Mg2+离子对动物群落变化的贡献率最大,是关键生态因子。(3)动物个体数量随土壤pH的增加而增加,随Mg2+、Cl-离子浓度的增加而下降。研究表明,土地利用变化引起的土壤盐分环境改变是驱动地面节肢动物群落演变的重要因素之一。 相似文献
16.
黄河三角洲刺槐根际与非根际细菌结构及多样性 总被引：4，自引：0，他引：4
为精确分析黄河三角洲刺槐根际与非根际土壤细菌群落定殖情况,本研究采用高通量测序方法对刺槐根际与非根际土壤细菌结构及多样性进行了研究。研究表明,根际土壤细菌共有36门214属,非根际土壤细菌共有33门153属。变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)细菌丰度超过15%,是根际与非根际土壤中的优势菌落。根际与非根际土壤中酸杆菌门、硝化螺旋菌门(Nitrospirae)丰度差异显著。根际土壤中红游动菌属(Rhodoplanes)、溶杆菌属(Lysobacter)、热单胞菌属(Thermomonas)、链霉菌属(Streptomyces)及非根际土壤中红游动菌属、溶杆菌属、链霉菌属、Kaistobacter细菌丰度超过4%。根际土壤中固氮菌丰度显著高于非根际土壤,解磷、解钾细菌丰度差异不显著。根际与非根际土壤细菌Chao丰富度分别为2 054、2 376,差异显著。根际与非根际土壤细菌之间的权重(Weighted Unifrac)距离在0.12~0.25之间。综上所述,黄河三角洲刺槐根际与非根际土壤细菌结构具有一定差异,多样性差异显著。 相似文献
17.
18.
John Farmer Bin Zhang Xinxin Jin Peng Zhang 《Archives of Agronomy and Soil Science》2017,63(2):230-241
The objective of this study was to access the effect of long-term fertilization and film mulching application on soil properties and bacterial community structure. We used 16S rRNA gene to investigate soil bacterial community composition by high through-put sequencing. The results demonstrated that predominant groups in the bacterial community were: Proteobacteria, Bacteroidetes, Actinobacteria, Acidobacteria, Firmicutes, Planctomycetes, Gemmatimonadetes, Verrucomicrobia, Chloroflexi and Cyanobacteria. Long-term fertilization of combined manure and nitrogen application caused significant decrease in soil bacterial diversity and richness compared to non-fertilization control, though manure fertilization alone played a significant role in restoring bacterial diversity. Film mulching and manure fertilization significantly increased the relative abundances of soil bacterial groups mentioned above. Furthermore, film mulching played significant role in shaping the bacterial community structure regardless of fertilization. Redundancy analysis (RDA) showed that soil moisture content, pH, total N and soil organic C had significant (P < 0.05) influence on dominant bacterial groups. Altogether, plastic film mulching and manure application prevented loss of soil bacterial diversity and abundance during long-term fertilization. These findings showed the detrimental use of combined manure and nitrogen fertilization to soil microbes and the useful application of manure fertilization coupled with film mulching to soil biodiversity in long-term fertilization experiments. 相似文献
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基于高通量测序的两种植被恢复类型根际土壤细菌多样性研究 总被引：21，自引：1，他引：20
为研究库布齐沙地生态恢复过程中不同植被恢复类型土壤微生物细菌群落结构、多样性的变化特征,以流动沙地为对照,运用高通量测序技术,对自然恢复的油蒿群落、人工种植的中间锦鸡儿群落根际和非根际土壤细菌多样性进行了研究,并分析了土壤理化性质对其分布的影响。结果表明:(1)与流沙对照相比,两种植被恢复类型对细菌多样性产生了正效应,细菌群落丰度、多样性和均匀度明显增加。其中,自然恢复的油蒿群落土壤细菌丰度高于人工种植的中间锦鸡儿群落;(2)变形菌门、酸杆菌门和放线菌门为研究区土壤中的优势细菌类群,其中变形菌门在各样地丰度比例最高,变形菌的4个亚群变化趋势一致,α-变形菌相对含量在油蒿和中间锦鸡儿群落根际土壤中明显增加,尤其是自然恢复的油蒿群落根际土壤中α-变形菌得到了很好的恢复;(3)土壤有机质、全氮、速效氮、速效钾含量和土壤含水量是影响土壤细菌群落丰度和多样性的主要土壤因子,典型相关分析表明土壤有机质、全氮、全钾、速效钾、速效氮含量对于研究区土壤细菌群落遗传多样性的变化起着重要作用。 相似文献