共查询到18条相似文献,搜索用时 750 毫秒
1.
咪唑类化合物KK-42对家蚕蛹发育的影响 总被引:1,自引:0,他引:1
本试验以家蚕蛹为材料,研究了咪唑类化合物KK-42对蛹发育包括卵巢发育的影响。试验结果表明:KK-42在蛹龄36小时之前处理有推迟复眼着色和羽化的作用,而且以复眼着色延迟更明显。KK-42对卵巢重和造卵数也有明显的抑制作用,KK-42对蛹和卵巢发育的抑制作用可通过注射β-蜕皮甾酮恢复,而由于注射保幼激素类似物被加强。同时还就KK-42的作用机理和卵巢发育的激素调节机制作了讨论。 相似文献
2.
柞蚕卵黄原蛋白的合成与转运 总被引:1,自引:0,他引:1
本文用免疫扩散和脂蛋白染色方法证实柞蚕印黄原蛋白系雌性所特有.卵黄原蛋白的免疫学一致性也得到确定.柞蚕卵黄原蛋白在进人预蛹期开始出现于雌体血淋巴中.在整个蛹滞育期间.卵黄原蛋白含量约为血淋巴总蛋白的50%;在成虫发育期印黄原蛋白含量剧降,与此同时.卵黄蛋白含量则激增,约占卵黄总蛋白的80%.卵巢摘除导致血淋巴印黄原蛋白含量剧增. 相似文献
3.
4.
以柞蚕5龄幼虫为材料添食柞蚕微孢子虫(Nosema pernyi,Np),通过测定与分析不同性别5龄幼虫血淋巴蛋白质含量和组成变化的差异,为探究柞蚕对柞蚕微孢子虫感染的免疫应答提供依据。感染柞蚕微孢子虫的不同性别柞蚕5龄幼虫的血淋巴蛋白质含量随蚕体发育进程出现不同的变化:雌性个体与雄性个体分别在添食Np后24 h与48 h,血淋巴蛋白质含量极显著增加(P<0.01),且持续至添食后96 h,其中雌性个体的血淋巴蛋白质含量又极显著高于雄性个体(P<0.01);在添食Np后120 h,雌、雄个体的血淋巴蛋白质含量与对照组无显著性差异(P>0.05)。SDS-PAGE分析表明,添食Np后的柞蚕5龄幼虫血淋巴蛋白质组成与对照组基本一致,均显示出20条分子质量在20.1~97.2 kD的蛋白条带,其中雌、雄个体在添食后96 h,大小约44 kD的蛋白条带明显加深,大小约28 kD的蛋白条带雌性个体在添食后144 h、雄性个体在添食后120 h明显加深,且雄性个体在添食后120~168 h大小约42 kD的蛋白条带明显加深,但在添食后192 h与对照组无明显差异。研究结果说明Np侵染后,柞蚕5龄幼虫血淋巴蛋白质的含量及组成均产生了一定的变化,并且雌雄个体间的变化存在差异。 相似文献
5.
用人工饲料无菌饲育方式 ,分析维生素B6(VB6)对家蚕丝腺生长、血淋巴蛋白质浓度、血淋巴游离氨基酸组成变化及氮代谢终产物尿酸形成的影响。结果表明 :5龄期家蚕在缺乏VB6条件下 ,丝腺生长严重受阻碍 ,血淋巴蛋白质不能正常合成 ,使体内氨基酸转化利用率下降 ,氮代谢终产物尿酸的排泄量增加 ,血淋巴中部分游离氨基酸浓度下降 ,特别是合成丝蛋白质主要成分的Ala、Ser和Tyr等蚕体非必需氨基酸的组成比率显著减少 ,而Gly、Orn和Cysta等的浓度增加异常 ,导致血淋巴中游离氨基酸组成动态平衡破坏 ,显示蚕体内因VB6缺乏引起氨基酸代谢失调。 相似文献
6.
<正> 昆虫血淋巴是细胞外体液,浸润着虫体内所有组织和细胞。全变态昆虫的蛹期是重要的变态期,在此期间,进行着幼虫组织的分解和成虫组织形成。血淋巴中蛋白质种类的变化情况,是昆虫生理学者关注的问题之一。柞蚕是典型全变态昆虫,柞蚕蛹期里翅芽发育,与此同时,在雄蛹中睾丸进一步发育成熟,而在雌蛹中卵巢开始发育,直至完全成热。因此,有必要分析柞蚕蛹期血淋巴蛋白质种类及其消长变化特点,为逐步深入阐明蛹期成虫器官芽生长发育过程中,利用血淋巴中的蛋白质致使血淋巴蛋白质种类出现有关变化,提供分析资料。 关于昆虫血淋巴蛋白质的分析工作,过 相似文献
7.
8.
9.
VB6对家蚕氮代谢的影响 总被引:1,自引:1,他引:0
用人工饲料无菌饲育方式,分析维生素B6(VB6)对家蚕丝腺生长、血淋巴蛋白质浓度、血淋巴游离氮基酸组成变化及氮代谢终产物尿酸形成的影响。结果表明:5龄期家蚕在缺乏VB6条件下,丝腺生长严重受阻碍,血淋巴蛋白质不能正常合成,使体内氨基酸转化利用率下降,氮代谢终产物尿酸的排泄量增加,血淋巴中部分游离氮基酸浓度下降,特别是合成丝蛋白质主要成分的Ala、Ser和Tyr等蚕体非必需氨基酸的组成比率显著减少,而Gly、Orn和Cysta等的浓度增加异常,导致血淋巴中游离氨基酸组成动态平衡破坏,显示蚕体内固VB6缺乏引起氨基酸谢代调。 相似文献
10.
11.
家蚕突变矮小卵血液蛋白及卵黄蛋白的研究 总被引:2,自引:0,他引:2
对家蚕突变矮小卵 (emi)品种在卵母细胞形成时期的血液、卵巢及卵中的总蛋白进行SDS PAGE发现 :在emi的卵巢及卵中含有卵黄蛋白 (Vn)、卵特异性蛋白 (ESP)和大部分的低分子量脂蛋白 (LP) ;emi蚕在蛹期和蛾期的血液中有卵黄原蛋白 (Vg)和LP ,蛹后期大部分emi血液中的Vg和LP含量逐渐减少 ,化蛾时血液中仅含少量的Vg和LP ,而少数emi个体的卵母细胞大量退化 ,在蛹后期和蛾期血液中还含有大量的LP和较多的Vg ;emi蚕从吐丝当天到蛾期的血液中的LP中有分子量为 30kD的蛋白带 ,在卵中却没有。以上结果表明 ,emi基因可能不仅决定卵大小 ,还可能对LP的不同成分的吸收 (如不能吸收LP中分子量为 30kD的蛋白 )有关 ,推测这可能是导致emi个体大部分不能正常发育的原因之一 相似文献
12.
A determination of age of the prepubertal gilt at which ovaries affect uterine growth is necessary before establishing the extent to which length of uterus is influenced by inherent differences, as opposed to those due to ovarian secretions. In Exp. 1 and 2, the effect of presence of ovaries on uterine growth was determined following ovariectomy in 186 crossbred gilts. The uterus was examined 40 or 80 d after ovariectomy for length, weight and diameter. Growth of uterine horns in gilts from 20 to 60 d of age was equal with or without ovaries. Uterine horns in ovariectomized gilts continued to grow slowly from 60 to 140 d of age and then remained static to 180 d of age. Uterine horns in gilts with ovaries increased rapidly in length, weight and diameter, with concomitant increase in ovarian weight between 100 and 180 d of age. In Exp. 3, uterine growth and ovarian compensation after unilateral ovariectomy and hysterectomy at 60 d of age were determined in 85 crossbred gilts from 60 to 180 d of age to evaluate the unilateral ovariohysterectomy model for studying association of uterine length before puberty and subsequent uterine capacity. In response to removal of an ovary and a uterine horn, the remaining ovary compensated, but the remaining uterine horn did not. This study demonstrated that the ovaries did not influence uterine growth until after 60 d of age and that unilateral ovariohysterectomy could be performed as early as 60 d of age without altering consequent normal uterine growth. 相似文献
13.
【目的】构建产蛋前后各期伊犁鹅卵巢转录组文库,结合生物信息学分析揭示不同产蛋期伊犁鹅卵巢组织差异表达基因,并鉴定出影响鹅卵巢发育的关键基因,为伊犁鹅的繁殖调控提供理论参考。【方法】选择处于开产期(KL组)、产蛋期(CL组)及休产期(XL组)的伊犁鹅各4只,屠宰后取其卵巢组织,用于构建卵巢转录组文库。通过新基因挖掘、基因差异表达、基因注释以及蛋白互作网络分析筛选出与鹅卵泡发育相关的候选基因;随机挑选8个差异表达基因,应用实时荧光定量PCR验证其表达情况。【结果】伊犁鹅卵巢组织学结果表明,在开产期时,伊犁鹅卵巢表面有大量初级卵泡,而产蛋期卵巢则显示出卵泡的层级性,在休产期卵巢中可观察到卵泡出现向内凹陷、闭锁的现象。通过转录组测序(RNA-Seq),从构建的12个伊犁鹅卵巢cDNA文库中获得有效读数57 811 186~85 328 377条,Q30值均>93.38%,每个样品所产的测序读数于鹅参考基因组上的比对率在82.79%~89.24%。在卵巢中注释的新基因共有1 112个,单核苷酸多态性(SNP)位点总数为1 642 273~2 425 069个;SNP和插入缺失(InDel)均主要注释于内含子区域;各时期的可变剪切类型主要集中为最后1个外显子可变剪切(TSS)及第1个外显子可变剪切(TTS)。在KL vs CL、XL vs CL及XL vs KL组中分别有337、1 136和525个差异表达基因,共有差异表达基因为α2A肾上腺素能受体(ADRA2A)、表皮蛋白(CP)、非转移性黑色素瘤糖蛋白B (GPNMB)及α-1-抗胰蛋白酶样(LOC106033756)。GO功能富集分析发现,差异表达基因主要富集在肽基酪氨酸磷酸化的正调控、细胞黏附及质膜外侧等过程。KEGG通路富集分析发现,差异表达基因主要显著富集于神经活性配体-受体相互作用、ECM-受体相互作用及类固醇生物合成等。结合蛋白互作网络分析,筛选到与鹅卵巢发育相关的潜在调控因子Bruton’s酪氨酸激酶(BTK)、血小板源性生长因子受体α(PDGFRA)、整合素β3(ITGB3)等。实时荧光定量PCR结果显示,RNA-Seq结果准确可靠。【结论】本研究揭示了产蛋前后各期伊犁鹅卵巢组织中的基因表达差异,筛选到影响伊犁鹅卵巢发育的神经活性配体-受体相互作用、ECM-受体相互作用、类固醇生物合成等重要通路与BTK、PDGFRA和ITGB3等关键候选基因,为了解鹅卵巢组织调控产蛋性能的分子机制提供了理论支撑。 相似文献
14.
柞蚕卵黄磷蛋白的鉴定、纯化及其在卵形成和胚胎发育中的变化 总被引:1,自引:1,他引:0
应用聚丙烯酰胺凝胶电泳、离子交换纤维素柱层析和薄层扫描等方法鉴定、纯化了柞蚕卵黄磷蛋白,并考察了该蛋白质在卵形成和胚胎发育期间的变化.结果表明:(1)发育卵巢和成熟卵内存在着一种卵黄磷蛋白(Vtn),其前体是雌蛹血淋巴内的卵黄原蛋白(Vg),该蛋白质具有雌特异性.(2)Chino等的方法也适用于以柞蚕卵为材料纯化Vtn,初步纯化的Vtn亚单位分子量是200KDa.(3)后蛹期,发育卵巢中可溶性总蛋白和Vtn的相对百分含量都迅速上升,至卵粒成熟期达最大值.(4)胚胎发育前期,卵可溶性总蛋白和Vtn的相对百分含量基本维持原水平;胚胎发育后期,二者都急剧地减少;孵化时卵可溶性总蛋白含量和Vtn的相对含量分别降至刚产下时的50%和10%以下. 相似文献
15.
Our study was conducted to assess the follicular development and availability of sound ovarian oocytes for in vitro production (IVP) of embryos in pre‐pubertal cats. The relationship between body and ovarian weight was examined in 93 cats. The results revealed that ovarian weight rapidly increased until 100 days of estimated age. By histological evaluation of ovaries obtained from 11 pre‐pubertal cats with estimated age of <20, 20–40 and 100–120 days, it was clarified that the increase in ovarian weight during kitten growth accompanied the increase in the number and size of antral follicles. The follicular diameter and percentage of normal oocytes in secondary/antral follicles also increased as estimated age (body weight) increased. The oocytes obtained from pre‐pubertal cats with 100–120 days of estimated age were used for IVP of embryos. The results showed that the success rates of in vitro maturation, in vitro fertilization and development to blastocysts after in vitro culture in pre‐pubertal cats were lower than in sexually mature cats. However, the percentage of blastocysts based on the cleaved embryos and cell number of blastocysts in pre‐pubertal cats were comparable to those in mature cats. In conclusion, these results suggest that the ovaries of pre‐pubertal cats with ≥100 days of age contain oocytes with in vitro developmental competence to blastocysts. 相似文献
16.
17.
Comparative effects of etomidate and its fluoro analogue, R 8110 on testicular, adrenal and ovarian steroid biosynthesis 总被引:1,自引:0,他引:1
R. DE COSTER W. WOUTERS D. BEERENS C. HAELTERMAN R. DOOLAEGE N. GOEMINNE M. KREKELS 《Journal of veterinary pharmacology and therapeutics》1988,11(4):345-353
The effects, of etomidate and of its fluoro analogue, R 8110, on adrenal, testicular and ovarian steroid biosynthesis were compared using cultures of guinea-pig adrenal, rat adrenal capsular, rat testicular and rat ovarian granulosa cells. At a concentration of 100 nM, etomidate inhibited the adrenal 11-hydroxylation of glucocorticoid and mineralocorticoid biosyntheses, producing a decrease in cortisol and corticosterone and an accumulation of 11-deoxycortisol and 11-deoxycorticosterone in guinea-pig adrenal and rat capsular adrenal cell suspensions, respectively. At higher concentrations (greater than 10(-6) M), etomidate also inhibited ovarian oestradiol production, testicular androgen formation and ovarian progesterone synthesis. The latter action suggests an effect on ovarian aromatase, on testicular 17 alpha/17,20-lyase activities and finally on cholesterol side-chain cleavage. The fluoro analogue of etomidate, R 8110, was ten times less potent as an inhibitor of 11-hydroxylation and affected progesterone formation only slightly in adrenal cell suspensions. Testosterone production was less affected by R 8110 than by etomidate. The increase of progestins suggests that the 17 alpha/17,20-lyase activities are the most sensitive testicular enzymatic reactions to R 8110. For inhibition of ovarian oestradiol production, R 8110 was twenty times more potent than etomidate. 相似文献