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1.
A laboratory incubation experiment was conducted to demonstrate that reduced availability of CO2 may be an important factor limiting nitrification. Soil samples amended with wheat straw (0%, 0.1% and 0.2%) and (15NH4)2SO4 (200 mg N kg–1 soil, 2.213 atom% 15N excess) were incubated at 30±2°C for 20 days with or without the arrangement for trapping CO2 resulting from the decomposition of organic matter. Nitrification (as determined by the disappearance of NH4+ and accumulation of NO3–) was found to be highly sensitive to available CO2 decreasing significantly when CO2 was trapped in alkali solution and increasing substantially when the amount of CO2 in the soil atmosphere increased due to the decomposition of added wheat straw. The co-efficient of correlation between NH4+-N and NO3–-N content of soil was highly significant (r =0.99). During incubation, 0.1–78% of the applied NH4+ was recovered as NO3– at different incubation intervals. Amendment of soil with wheat straw significantly increased NH4+ immobilization. From 1.6% to 4.5% of the applied N was unaccounted for and was due to N losses. The results of the study suggest that decreased availability of CO2 will limit the process of nitrification during soil incubations involving trapping of CO2 (in closed vessels) or its removal from the stream of air passing over the incubated soil (in open-ended systems).  相似文献   

2.
Alternative silvicultural systems were introduced in Coastal Western Hemlock forests of British Columbia, Canada, to reduce disturbance incurred by conventional clear-cutting and to maintain the forest influence on soil nutrient cycling. As we hypothesized, in situ pools and net mineralization of NH4 + were lower under no and low disturbance (old-growth forest and shelterwood) compared to clear-cuts (high disturbance); in situ pools and net production of NO3 − were very low across all treatments. Gross transformation rates of NH4 + increased while those of NO3 − decreased with increasing disturbance, suggesting that these processes were uncoupled. We conclude that shelterwood harvesting reduces the impact on forest floor NH4 + cycling compared to clear-cutting, and that neither low nor high disturbance intensity results in substantial NO3 − accumulation, as what occasionally occurs in other ecosystems. We hypothesize that the uncoupling of NH4 + and NO3 − dynamics may be due to the predominance of heterotrophic nitrification by lignin-degrading fungi that oxidize organic N rather than NH4 +–N, and whose activities are suppressed at high NH4 + concentrations.  相似文献   

3.
 Seedlings of Chinese pine (Pinus tabulaeformis Carr.) were grown in a growth chamber using a sterilized soil/sand/vermiculite mixture, and were inoculated with the ectomycorrhizal fungus Cenococcum graniforme (Sow.) Ferd. and Winge. The function of external mycelium of ectomycorrhiza for uptake and transport of N from 15N-labeled NH4 + and NO3 – for plant nutrition was evaluated under well-watered conditions and water stress. The pots comprised two-compartments, whereby the penetration of roots was prevented by a nylon mesh bag which the mycorrhizal hyphae were allowed to pass in order to colonize the rest of the pot. 15N-labeled NH4 + or NO3 – was applied to the area of the pot to which the root had no access. At harvest, the 15NH4 + concentration in plant tissues was significantly promoted by the formation of mycorrhizae both under well-watered conditions and water stress. The 15NO3 – concentration was reduced by water stress and increased by mycorrhizal formation. The enhancement of 15NO3 – uptake caused by mycorrhizal formation was more evident under water stress than under well-watered conditions. The external mycelia of the ectomycorrhizae took up and transported NH4 + and NO3 – from the soil to the plant, thereby improving plant nutrition and growth, in addition to helping the plants to avoid the effects of water stress. Received: 31 October 1997  相似文献   

4.
 The objectives of this work were to evaluate the inhibitory action on nitrification of 3,4-dimethylpyrazole phosphate (DMPP) added to ammonium sulphate nitrate [(NH4)2SO4 plus NH4NO3; ASN] in a Citrus-cultivated soil, and to study its effect on N uptake. In a greenhouse experiment, 2 g N as ASN either with or without 0.015 g DMPP (1% DMPP relative to NH4 +-N) was applied 6 times at 20-day intervals to plants grown in 14-l pots filled with soil. Addition of DMPP to ASN resulted in higher levels of NH4 +-N and lower levels of NO3 –-N in the soil during the whole experimental period. The NO3 –-N concentration in drainage water was lower in the ASN plus DMPP (ASN+DMPP)-treated pots. Also, DMPP supplementation resulted in greater uptake of the fertilizer-N by citrus plants. In another experiment, 100 g N as ASN, either with or without 0.75 g DMPP (1% DMPP relative to NH4 +-N) was applied to 6-year-old citrus plants grown individually outdoors in containers. Concentrations of NH4 +-N and NO3 –-N at different soil depths and N distribution in the soil profile after consecutive flood irrigations were monitored. In the ASN-amended soil, nitrification was faster, whereas the addition of the inhibitor led to the maintenance of relatively high levels of NH4 +-N and NO3 –-N in soil for longer than when ASN was added alone. At the end of the experiment (120 days) 68.5% and 53.1% of the applied N was leached below 0.60 m in the ASN and ASN+DMPP treatments, respectively. Also, leaf N levels were higher in plants fertilized with ASN+DMPP. Collectively, these results indicate that the DMPP nitrification inhibitor improved N fertilizer efficiency and reduced NO3 – leaching losses by retaining the applied N in the ammoniacal form. Received: 31 May 1999  相似文献   

5.
Background, aim, and scope  Hoop pine (Araucaria cunninghamii) is a nitrogen (N) demanding indigenous Australia softwood species with plantations in Southeast Queensland, Australia. Soil fertility has declined with increasing rotations and comparison study of N cycling between hoop pine plantations, and adjacent native forest (NF) is required to develop effective forest management for enhancing sustainable forest production and promoting environmental benefits. Field in situ mineral 15N transformations in these two forest ecosystems have not been studied. Hence, the present study was to compare the differences in soil nutrients, N transformations, 15N fluxes, and fate between the hoop pine plantation and the adjacent native forest. Materials and methods  The study sites were in Yarraman State Forest (26°52′ S, 151°51′ E), Southeastern Queensland, Australia. The in situ core incubation method was used in the field experiments. Mineral N was determined using a LACHAT Quickchem Automated Ion Analyzer. 15N were performed using an isotope ratio mass spectrometer with a Eurovector elemental analyzer. All statistical tests were carried out by the SPSS 11.0 for Windows statistical software package. Results  Soil total C and N were significantly higher in the NF than in the 53-year-old hoop pine plantation. Concentrations of NO3 – were significantly higher in the NF soil than in the plantation soil. The plantation soil had significantly higher 15N and 13C natural abundances than the NF soil. The NF soil had significantly lower C/N ratios than the plantation soil. NO3 ––N was dominated in mineral N pools in both NF and plantation soils, accounting for 91.6% and 70.3% of the total mineral N pools, respectively. Rates of net nitrification and net N mineralization were, respectively, four and three times higher in the NF soil than in the plantation soil. The 15NO3 ––N and mineral 15N were significantly higher in the NF soil than in the plantation soil. Significant difference in 15NH4 +–N was found in the NF soil before and after the incubation. Discussion  The NF soil had significantly higher NO3 ––N, mineral N, total N and C but lower δ15N, δ13C, and C/N ratios than the plantation soil. Moreover, the rates of soil net N mineralization and nitrification were significantly higher, but ammonification rate was lower in the NF than in the plantation. The NF soil had many more dynamic N transformations than the plantation soil due to the combination of multiple species and layers and, thus, stimulation of microbial activity and alteration of C and N pool sizes in favor of the N transformations by soil microbes. The net rate of N and 15N transformation demonstrated differences in N dynamic related to the variation in tree species between the two ecosystems. Conclusions  The change of land use and trees species had significant impacts on soil nutrients and N cycling processes. The plantation had larger losses of N than the NF. The NO3 ––N and 15NO3 ––N dominated in the mineral N and 15N pools in both forest ecosystems. Recommendations and perspectives  Native forest soil had strong N dynamic compared with the plantation soil. Composition of multiple tree species with different ecological niches in the plantation could promote the soil ecosystem sustainability. The 15N isotope dilution technique in the field can be quite useful for studying in situ mineral 15N transformations and fate to further understand actual N dynamics in natural forest soils.  相似文献   

6.
The response of terrestrial ecosystems to elevated atmospheric CO2 is related to the availability of other nutrients and in particular to nitrogen (N). Here we present results on soil N transformation dynamics from a N-limited temperate grassland that had been under Free Air CO2 Enrichment (FACE) for six years. A 15N labelling laboratory study (i.e. in absence of plant N uptake) was carried out to identify the effect of elevated CO2 on gross soil N transformations. The simultaneous gross N transformation rates in the soil were analyzed with a 15N tracing model which considered mineralization of two soil organic matter (SOM) pools, included nitrification from NH4+ and from organic-N to NO3− and analysed the rate of dissimilatory NO3− reduction to NH4+ (DNRA). Results indicate that the mineralization of labile organic-N became more important under elevated CO2. At the same time the gross rate of NH4+ immobilization increased by 20%, while NH4+ oxidation to NO3− was reduced by 25% under elevated CO2. The NO3− dynamics under elevated CO2 were characterized by a 52% increase in NO3− immobilization and a 141% increase in the DNRA rate, while NO3− production via heterotrophic nitrification was reduced to almost zero. The increased turnover of the NH4+ pool, combined with the increased DNRA rate provided an indication that the available N in the grassland soil may gradually shift towards NH4+ under elevated CO2. The advantage of such a shift is that NH4+ is less prone to N losses, which may increase the N retention and N use efficiency in the grassland ecosystem under elevated CO2.  相似文献   

7.
Agricultural systems that receive high amounts of inorganic nitrogen (N) fertilizer in the form of either ammonium (NH4+), nitrate (NO3−) or a combination thereof are expected to differ in soil N transformation rates and fates of NH4+ and NO3−. Using 15N tracer techniques this study examines how crop plants and soil microbes vary in their ability to take up and compete for fertilizer N on a short time scale (hours to days). Single plants of barley (Hordeum vulgare L. cv. Morex) were grown on two agricultural soils in microcosms which received either NH4+, NO3− or NH4NO3. Within each fertilizer treatment traces of 15NH4+ and 15NO3− were added separately. During 8 days of fertilization the fate of fertilizer 15N into plants, microbial biomass and inorganic soil N pools as well as changes in gross N transformation rates were investigated. One week after fertilization 45-80% of initially applied 15N was recovered in crop plants compared to only 1-10% in soil microbes, proving that plants were the strongest competitors for fertilizer N. In terms of N uptake soil microbes out-competed plants only during the first 4 h of N application independent of soil and fertilizer N form. Within one day microbial N uptake declined substantially, probably due to carbon limitation. In both soils, plants and soil microbes took up more NO3− than NH4+ independent of initially applied N form. Surprisingly, no inhibitory effect of NH4+ on the uptake and assimilation of nitrate in both, plants and microbes, was observed, probably because fast nitrification rates led to a swift depletion of the ammonium pool. Compared to plant and microbial NH4+ uptake rates, gross nitrification rates were 3-75-fold higher, indicating that nitrifiers were the strongest competitors for NH4+ in both soils. The rapid conversion of NH4+ to NO3− and preferential use of NO3− by soil microbes suggest that in agricultural systems with high inorganic N fertilizer inputs the soil microbial community could adapt to high concentrations of NO3− and shift towards enhanced reliance on NO3− for their N supply.  相似文献   

8.
ABSTRACT

Plant nitrogen (N)-acquisition strategy affects soil N availability, community structure, and vegetation productivity. Cultivated grasslands are widely established to improve degraded pastures, but little information is available to evaluate the link between N uptake preference and forage crop biomass. Here an in-situ 15N labeling experiment was conducted in the four cultivated grasslands of Inner Mongolia, including two dicots (Medicago sativa and Brassica campestris) and two monocots (Bromus inermis and Leymus chinensis). Plant N uptake rate, shoot- and root biomass, and concentrations of soil inorganic-N and microbial biomass-N were measured. The results showed that the root/shoot ratios of the dicots were 2.6 to 16.4 fold those of the monocots. The shoot N concentrations of the dicots or legumes were 40.6% to 165% higher than those of the monocots or non-legumes. The four forage crops in the cultivated grassland preferred to uptake more NO3?-N than NH4+-N regardless of growth stages, and the NH4+/NO3? uptake ratios were significantly lower in the non-legumes than in the legumes (p < 0.05). Significant differences in the NH4+-N rather than NO3?-N uptake rate were observed among the four forages, related to plant functional types and growth stages. The NH4+ uptake rate in the perennial forages exponentially decreased with the increases in shoot-, root biomass, and root/shoot ratio. Also, the plant NH4+/NO3? uptake ratio was positively correlated with soil NH4+/NO3? ratio. Our results suggest that the major forage crops prefer to absorb soil NO3?-N, depending on soil inorganic N composition and belowground C allocation. The preferential uptake of NO3?-N by forages indicates that nitrate-N fertilizer could have a higher promotion on productivity than ammonium-N fertilizer in the semi-arid cultivated grassland.  相似文献   

9.
Core lysimeters containing undisturbed coarse sandy soil (from grassland) were amended with a high rate of anaerobically digested sewage sludge (equivalent to >1,000 t ha–1). Water, at a rate equivalent to the mean weekly rainfall for the soil, was applied to amended and control lysimeters for 30 weeks and the leachate analysed for anions and cations. Lysimeters were also destructively sampled at intervals throughout the experiment and soil samples were analysed for extractable NH4+-N, NO3–-N and PO43–-P. Ammonium N leached for about 11 weeks from the amended lysimeters, then abruptly stopped. A similar amount of NO3–-N leached, but leaching was continuing when the experiment finished. The control lysimeters leached as much NO3–-N as those that were amended, but no NH4+-N. The amended lysimeters also leached NO2–-N. Negligible PO43–-P, but large amounts of SO42– were leached from the amended lysimeters. Concentrations of extractable NH4+-N and PO43–-P were very high in the amended soils, but NO3–-N concentrations remained low throughout the experiment, indicating that nitrification rates were low and/or that denitrification rates were high.  相似文献   

10.
Replacing new corn genotypes in agricultural practices requires adequate information on the reaction of the selected hybrids to Cd uptake in Cd-polluted soil and an understanding of interactions with N fertilizers. A 2 × 2 × 3 factorial pot experiment with limed soil (pH 8), two maize (Zea mays) hybrids (Pioneer cultivar yellow and Pioneer cultivar white), two N fertilization forms (NH4 + and NO3 ?) and three Cd exposures (0, 2 and 5 mg kg?1 soil) was conducted under greenhouse conditions. Shoot dry mass increased significantly with NH4 + nutrition compared with NO3 ? nutrition in both maize hybrids, with greater negative influence of Cd application combined with NH4 + nutrition. The yellow cultivar had significantly greater shoot dry mass and lower Cd uptake than the white cultivar. Both hybrids exhibited similar N uptake in shoots and roots, with the exception of yellow cultivar with NH4 + nutrition without Cd application. NO3 ? nutrition always decreased Cd uptake in both cultivars compared with NH4 + nutrition. The N balance (mean across cultivars and Cd supply) after harvest showed most N uptake with NH4 + nutrition (63.4%) and Nmin remains in the soil with NO3 ? nutrition (48.7%). Soil pH decreased more with NH4 + (?0.95 pH units) than NO3 ? nutrition (?0.21).  相似文献   

11.
Abstract

A test for nitrogen (N) deficiency in plants showed that rate of uptake of 15N by excised roots was inversely related to the amount of N supplied to the growing plant. Deficiency was successfully identified from the rate of uptake of 15NH4 + from solution in a two hour period, and this was adopted in the early development of the method. In this study, modifications were tested in a sensitivity analysis, to compare 15NH4 + and 15NO3 ? uptake for different times of immersion and with different washing treatments, to determine the most sensitive method. Common bentgrass (Agrostis capillaris L.), grown in sand culture at a range of N concentrations from deficient to optimum, was the test plant. Although uptake by roots from N deficient treatments was always significantly higher for both ammonium (NH4) and nitrate (NO3), the uptake of NH4 was two to five times higher than that of NO3. The rate of uptake of both ions was approximately linear over 20 mins, one hour, or two hours. Although a two hour period of immersion would be necessary in some cases to obtain sufficient 15N in the roots for analysis, it should therefore be possible to interpolate, for direct comparison of rates of uptake by different species, and also with results from similar phosphorus (P) and potassium (K) bioassays, where radioisotope uptake is measured over a shorter time (15 min). The results suggest that the method initially adopted was the most sensitive to identify N deficiency.  相似文献   

12.
Availability and leaching of dissolved inorganic N (DIN = NH4+ + NO3-) in soil were measured in a periodically flooded forest of the Central Amazon floodplain (várzea) during one terrestrial phase. Special emphasis was on the effects of a legume and a non-legume tree species. NH4+-N accounted for more than 85% of DIN even at the end of the terrestrial phase although it decreased throughout the experimental period. While extractable NO3–-N was always low in the soil (less than 15% of DIN), the amount of leached NO3–-N was in the same range as NH4+-N. Under the legume trees mean DIN contents of the topsoil were higher than under the non-legume trees. DIN leaching from the topsoil (0–20 cm) was significantly higher under the legume trees than at the other sites, also indicating a higher N availability. Therefore, despite considerable leaching legume trees may be an important source of N supporting a high biomass production of the várzea forest.  相似文献   

13.
Nitrogen (N), ammonium (NH4+) and nitrate (NO3?), is one of the key determinants for plant growth. The interaction of both ions displays a significant effect on their uptake in some species. In the current study, net fluxes of NH4+ and NO3? along the roots of Picea asperata were determined using a Non-invasive Micro-test Technology (NMT). Besides, we examined the interaction of NH4+ and NO3? on the fluxes of both ions, and the plasma membrane (PM) H+-ATPases and nitrate reductase (NR) were taken into account as well. The results demonstrated that the maximal net NH4+ and NO3? influxes were detected at 13–15?mm and 8–10.5?mm from the root apex, respectively. Net NH4+ influx was significantly stimulated with the presence of NO3?, whereas NH4+ exhibited a markedly negative effect on NO3? uptake in the roots of P. asperata. Also, our results indicated that PM H+-ATPases and NR play a key role in the control of N uptake.  相似文献   

14.
The influence of nitrogen (N) forms and chloride (Cl) on solution pH and ion uptake in the hydroponic culture of Ageratum houstonianum [ammonium (NH4 +)‐tolerant] and Salvia splendens (NH4 +‐sensitive) for a period of 216 hours was investigated. The pH of the hydroponic solution (initially 6.50) containing either NH4 + or NH4 ++nitrate (NO3 ‐) was drastically lowered (3.08), whereas that of the same solution containing NO3 ‐ was raised (7.74). Solution pH changed more by ageratum than by salvia. The solution Cl‐ concentration did not influence pH significantly. However, addition of Cl‐ in the solution lowered transpiration rate in both NH4 + and NO3 ‐ treatments. Total N uptake was the greatest in the NH4 + + NO3 ‐ treatment and the lowest in the NO3 ‐treatment. In the NH4 + + NO3 ‐ treatment, NO3 ‐ uptake was suppressed by NH4 + (to about 50%), while NH4 + uptake was not affected by NO3 ‐. The rate of Cl‐ uptake was the lowest in the NH4 + treatment, but was similar in the NH4 + + NO3 ‐ and NO3 ‐ treatments. Uptake of potassium (K+), dihydrogen phosphate (H2PO4 ‐), sulfate (SO4 ‐2), manganese (Mn+2), and zinc (Zn+2) was significantly enhanced in the NH4 + treatment. The uptake rate of calcium (Ca+2) and magnesium (Mg+2) was the highest in the NO3 ‐ treatment. Absorption of copper (Cu+2) and boron (B) was not affected by N source. Ion uptake was more stable in the solution containing both NH4 + and NO3 ‐ than in the solution containing either NH4 + or NO3 ‐. The uptake rate of total N, NH4 +, NO3 ‐, Mn+2, Cu+2, and Zn+2 was higher, whereas that of Cl‐ and molybdenum (Mo) was lower in ageratum than in salvia. Amounts of total anion (TA) and total cation (TC) absorbed, the sum of TC and TA, and the difference between TC and TA (TC‐TA) were affected by N source, Cl‐ level, and their interactions. The NO3 treatment, as compared to the NH4 + or the NH4 + + NO3 ‐treatment, reduced total cation and anion uptake while increasing TC‐TA, especially in the absence of Cl‐. Plant tissue ion contents were also affected by N source and Cl‐ level.  相似文献   

15.
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16.
Solution pH, temperature, nitrate (NO3 ‐)/yammonium (NH4 +) ratios, and inhibitors effects on the NO3 ‐ and NH4 + uptake rates of coffee (Coffea arabica L.) roots were investigated in short‐term solution culture. At intermediate pH values (4.25 to 5.75) typical of coffee soils, NH4 + and NO3 ‐ uptake rates were similar and nearly independent of pH. Nitrate uptake varied more with temperature than did ammonium. Nitrate uptake increased from 0.05 to 1.01 μmol g‐1 FWh‐1 between 4 and 16°C, and increased three‐fold between 16 to 22°C. Between 4 to 22°C, NH4 + uptake rate increased more gradually from 1.00 to 3.25 μmol g‐1 FW h‐1. In the 22–40°C temperature range, NH4 + and NO3 ‐ uptake rates were similar (averaging 3.65 and 3.56 umol g‐1 FW h‐1, respectively). At concentrations ranging from 0.5 to 3 mM, NO3 ‐ did not influence NH4 + uptake rate. However, NO3 ‐ uptake was significantly reduced when NH4 + was present at 3 mM concentration. Most importantly, total uptake (NO3 ‐+NH4 +) at any NO3 ‐/NH4 + ratio was higher than that of plants fed solely with either NH4 + or NO4 ‐. Anaerobic conditions reduced NO3 ‐ and NH4 + uptake rate by 50 and 30%, respectively, whereas dinitrophenol almost completely inhibited both NH4 + and NO3 ‐ uptake. These results suggest that Arabica coffee is well adapted to acidic soil conditions and can utilize the seasonally prevalent forms of inorganic N. These observations can help optimizing coffee N nutrition by recommending cultural practices maintaining roots in the temperature range optimum for both NH4 + and NO3 ‐ uptake, and by advising N fertilization resulting in a balanced soil inorganic N availability.  相似文献   

17.
ABSTRACT

A pot experiment was conducted to study the growth and pungency of Allium cepa L. grown in Perlite as affected by colonization by the arbuscular mycorrhizal (AM) fungi Glomus versiforme and Glomus intraradices BEG141 and by ammonium:nitrate (NH4 +:NO3 ?) ratios of 3:1, 1:1, and 1:3 in 4 mM solutions. Plants were harvested when bulb formation commenced. In general, mycorrhizal colonization resulted in increased shoot dry weight, shoot length, sheath diameter, root nitrogen (N) and phosphorus (P) content (except with G. intraradices and a NH4 +:NO3 ? ratio of 1:3), shoot N and P concentrations (except with G. versiforme and a NH4 +:NO3= ratio of 3:1) and content. Plants inoculated with G. versiforme had higher growth parameters and N and P content than those with G. intraradices, whereas N and P concentrations showed the opposite trends. Growth parameters and N and P content of non-mycorrhizal plants were highest at a NH4 +:NO3= ratio of 1:3, while those of plants inoculated with G. versiforme or G. intraradices were highest at a ratio of NH4 +:NO3 ? 3:1 or 1:1. Neither mycorrhizal colonization nor proportion of inorganic N species significantly affected bulb enzyme-produced pyruvate or total or organic sulfur (S) concentrations in plant shoots. Colonization by AM fungi made a substantial contribution to onion growth and may not have been directly related to bulb pungency at early stages of plant growth. However, the influence of AM fungi on plant N and P metabolism may have implications for onion flavor at later stages of plant growth.  相似文献   

18.
Uptake and assimilation of inorganic N in young rice plants has been studied with labelled N (N-15). Depletion of the plants' carbohydrate content, obtained by a preceding dark period, resulted in a drastic reduction of NH4 +-N uptake. Plants exposed to low light intensity showed diminishing NH4 +-N uptake rates as compared with plants exposed to full light intensity, the latter showing constant NH4 +-N uptake rates during the whole experimental period. The percentage of labelled insoluble N in total labelled N was not significantly affected by a preceding dark period, whereas the low light intensity resulted in a lower proportion of insoluble N in roots and shoots. The incorporation of labelled N into the insoluble fraction (proteins, nucleic acids) was higher in plants fed with NH4 +-N than in those fed with NO3 -.

The uptake of NH4 +-N was not significantly affected by NO3 -, whereas the NO3- uptake rate was considerably reduced in the presence of NH4 +-N. Low energy status of plants affected the nitrate uptake more than the uptake of NH4 +-N. The results show that uptake and assimilation of inorganic N depend much on the energetic status of plants. Nitrate uptake and assimilation is more sensitive to low energy conditions than NH4 +-N.  相似文献   

19.
Summary The chloroform fumigation-incubation method (CFIM) was used to measure the microbial biomass of 17 agricultural soils from Punjab Pakistan which represented different agricultural soil series. The biomass C was used to calculate biomass N and the changes occurring in NH4 +-N and NO3 –-N content of soils were studied during the turnover of microbial biomass or added C source. Mineral N released in fumigated-incubated soils and biomass N calculated from biomass C was correlated with some N availability indexes.The soils contained 427–1240 kg C as biomass which represented 1.2%–6.9% of the total organic C in the soils studied. Calculations based on biomass C showed that the soils contained 64–186 kg N ha–1 as microbial biomass. Immobilization of NCO3 –-N was observed in different soils during the turnover of microbial biomass and any net increase in mineral N content of fumigated incubated soils was attributed entirely to NH4 +-N.Biomass N calculated from biomass C showed non-significant correlation with different N availability indexes whereas mineral N accumulated in fumigated-incubated soils showed highly significant correlations with other indexes including N uptake by plants.  相似文献   

20.
Summary The major agronomic concern with NH3 loss from urea-containing fertilizers is the effect of these losses on crop yields and N fertilizer efficiency. In this 2-year study, NH3 volatilization from surface-applied N fertilizers was measured in the field, and the effects of the NH3 losses detected on corn (Zea mays L.) and orchardgrass (Dactylis glomerata L.) yield and N uptake were determined. For corn, NH4NO3 (AN), a urea-AN solution (UAN), or urea, were surface-broadcast at rates of 0, 56 and 112 kg N ha–1 on a Plano silt loam (Typic Argiudoll) and on a Fayette silt loam (Typic Hapludalf). Urea and AN (0 and 67 kg N ha–1) were surface-applied to grass pasture on the Fayette silt loam. Significant NH3 losses from urea-containing N sources were detected in one of four corn experiments (12%–16% of applied N) and in both experiments with grass pasture (9%–19% of applied N). When these losses occurred, corn grain yields with UAN and urea were 1.0 and 1.5 Mg ha–1, respectively, lower than yields with AN, and orchardgrass dry matter yields with urea were 0.27 to 0.74 Mg ha–1 lower than with AN. Significant differences in crop N uptake between N sources were detected, but apparent NH3 loss based on N uptake differences was not equal to field measurements of NH3 loss. Rainfall following N application markedly influenced NH3 volatilization. In corn experiments, NH3 loss was low and yields with all N sources were similar when at least 2.5 mm of rainfall occurred within 4 days after N application. Rainfall within 3 days after N application did not prevent significant yield reductions due to NH3 loss from urea in grass pasture experiments.  相似文献   

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