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1.
In the present study, juvenile (live body weight, 54.3 ± 8.2 g), preadult (live body weight, 822.5 ± 33.9 g), and adult (live body weight, 1,562.8 ± 41.8 g) pacu, Piaractus mesopotamicus, were used to estimate their dietary essential amino acid (EAA) requirements using the whole-body amino acid (AA) pattern. The results showed that whole-body moisture, crude protein, total lipid, and ash contents expressed on a wet weight basis (%) were significantly different among the studied growth phases. No significant differences were observed in the dietary EAA requirements estimated for the three growth phases of pacu. These dietary EAA requirements were found to be different than those previously estimated for the same fish through its muscle AA pattern. Based on whole-body EAA to total EAA ratios {A/E ratios; [(each EAA/total EAA) × 1,000]}, EAA requirements were estimated to be histidine (0.42%), arginine (1.36%), threonine (0.82%), valine (0.90%), methionine (0.45%), isoleucine (0.83%), leucine (1.29%), phenylalanine (0.74%), lysine (1.64%), and tryptophan (0.14%) for pacu. These estimated requirements may serve as a reference line in the formulation of practical and experimental diets until dose–response-based optimum EAA requirements are available for pacu.  相似文献   

2.
The apparent digestibility coefficients (ADCs) of amino acids (AA), protein, and energy in six feed ingredients (Brazilian fish meal, soybean meal, corn gluten meal, alcoholic yeast, corn, and wheat bran) we evaluated for pacu juveniles. In general, all ingredients showed high digestibility values for all AA, and differences among ADCs of individual amino acid were detected ( P  < 0.01). Corn gluten, soybean, and fish meals had the highest ADCs of AA. The ADCs of protein in fish meal, yeast, and corn gluten meal should not be used as AA digestibility indicators, because those showed differences up to 6.7% between the ADCs of protein and AA. All ingredients had lower ADCs of energy than corn gluten meal ( P  < 0.01). Lysine was the first limiting amino acid in fish meal, corn gluten meal, wheat bran, and corn, and the second limiting amino acid in soybean meal, as methionine was the first limiting amino acid in soybean meal and yeast. However, the soybean meal was the best quality protein source, as it had the highest digestible essential amino acid index. This demonstrated that digestible amino acid values can be used to formulate practical diets for pacu, preventing potential deficiencies or excess that might cause environmental and economic losses.  相似文献   

3.
Oxygen consumption attributable to apparent heat increment (AHI) was measured in relation to varying essential amino acid proportions (EAA) infused into rainbow trout,Oncorhynchus mykiss (250–450 g), induced to swim at 1 BL s–1. Five diets, mimicking EAA concentrations in trout whole body protein, deficient in the branched chain amino acids (isoleucine, leucine and valine), containing unbalanced proportions of EAAs and supplying lysine in excessive and limiting proportions, were tested. Following infusion of the experimental diets, a significant increase in oxygen consumption was observed. Changes in plasma EAAs following infusion paralleled the time course of AHI (i.e., oxygen consumption). AHI represented the equivalent of 15–32% of the gross energy intake depending on dietary EAA composition. Diets supplying EAAs similar to trout whole body protein and limiting in lysine produced the lowest AHI values, indicating efficient utilization of dietary amino acids. Higher AHI values were associated with diets deficient in the branched chain amino acids and diets supplying lysine in excess. Duration of elevated metabolism was independent of both dietary composition and energy intake. Different proportions of EAAs in the diet can increase the energy expended as AHI. In an attempt to reduce the energy liberated as AHI, attention must be paid to the quality, quantity and balance of dietary EAAs.  相似文献   

4.
Aquafeed formulation is susceptible to affect amino acid (AA) availability for metabolic functions. Statistical models were applied to quantify the effect of dietary methionine level (from 6.01 to 16.17 g kg?1 dry matter) and form (free, coated or bound) on postprandial concentrations of plasma essential amino acid (EAA) in rainbow trout. Twelve diets were formulated with pea and soya protein concentrate or fish meal as the main protein ingredients and were supplemented or not with increasing amount of either crystalline or agar‐coated methionine. Fish were acclimatized to one of the 12 diets for 6 weeks before postprandial plasma sampling (six sampling points up to 36 h, seven fish each time), further analysed for EAA content. Using generalized additive models, we show that (i) dietary methionine level and form explained 74% postprandial methionine plasma variations and that (ii) the methionine dietary form and plasma concentrations significantly affected the plasma concentrations of the other EAAs. Finally, linear model revealed a positive relationship (R2 > 0.9) between plasma concentrations of the three branched‐chain AAs under the present experimental conditions. The results obtained add new information on the dietary effects on EAAs in the plasma availability and the interactions between them.  相似文献   

5.
A 6‐week feeding trial was conducted for determining the effects of dietary essential amino acids (EAA) deficiencies on growth performance and non‐specific immune responses in silvery‐black porgy juveniles (4.7 ± 0.1 g initial weight). Eleven isoproteic (ca. 47%) diets were formulated including a control diet containing the optimum quantity of EAA, and ten EAA‐deficient diets. All diets contained 36% fish meal and 18.5% crystalline EAA and non‐essential amino acids (NEAA) as the main source of dietary proteins. All the EAA and NEAA incorporated in the crystalline amino acids mixture of the control diet simulated the amino acids profile of the fish meal. The other 10 EAA‐deficient diets were formulated by the deletion of each of the 10 EAA (crystalline form) from the control diet and replaced by a mixture of NEAA for the adjustment of dietary nitrogen contents. At the end of the experiment, fish fed with threonine‐deficient diet showed the lowest survival rate (< .05), whereas growth performance decreased in fish fed all EAA‐deficient diets, although the reduction in body growth varied depending on the EAA considered. Plasma total protein decreased in all experimental groups except for fish fed the phenylalanine‐deficient diet. Fish fed with arginine‐ and lysine‐deficient diets had the lowest plasma C3, C4, lysozyme, total immunoglobulin and total superoxide dismutase activity (< .05). Present results indicated that lysine, methionine and threonine were the most limiting EAA in terms of growth performance; however, arginine, threonine and lysine were the most limiting EAA for innate immunity responses in silvery‐black porgy juveniles.  相似文献   

6.
A 6‐week feeding trial was conducted to estimate the optimum dietary essential amino acid (EAA) pattern for silvery‐black porgy juvenile based on the AA deletion method. Eleven isonitrogenous and isoenergetic diets were formulated containing 60% of fish meal nitrogen and 40% of crystalline AA nitrogen. In the control diet, the EAA profile was made similar to fish meal protein. Ten other diets were formulated similar to the control diet but replacing 40% of each EAA by a mixture of non‐essential amino acids. Triplicate groups of fish (initial body weight of 4.7 g) were handfed with the experimental diets, three times a day, to visual satiation, for 42 days. At the end of the trial, final body weight of all EAA‐deficient groups was lower than that of control group, ranging from 6.3% of reduction with arginine‐deficient diet to 39.4% of reduction with lysine‐deficient diet, relatively to the control group. Based on the relationship between nitrogen retention and EAA intake of the control and EAA‐deficient diets, the optimal dietary EAA profile for silvery‐black porgy juveniles was estimated to be (g 16/g N): arginine 5.3, lysine 6.0, threonine 5.2, histidine 2.5, isoleucine 4.6, leucine 5.4, methionine + cysteine 4.0 (in a diet containing 0.6 cysteine), phenylalanine + tyrosine 5.6 (in a diet containing 1.9 tyrosine), tryptophan 1.0 and valine 4.6.  相似文献   

7.
In order to determine the essential amino acid requirements (EAA) of striped bass Morone saxatilis , fillets were analysed to ascertain the relative amino acid concentrations for determining A/E ratios ((EAA/total EAA) × 1000)). Analysis of the striped bass fillets yielded the following concentrations of essential amino acids (g kg–1) and A/E ratios, respectively: arginine, 12.5, 115; histidine, 5.1, 47; isoleucine, 8.0, 74; leucine, 17.1, 157; lysine, 20.2, 186; methionine + cysteine, 9.2, 85; phenylalanine + tyrosine, 16.0, 147; threonine, 9.8, 90; tryptophan, 1.9, 18; and valine, 9.1, 84. In two experiments, diets with graded levels of EAA were fed to striped bass weighing 111 ± 3 g and 790 ± 122 g per fish, respectively. In both experiments, the dietary A/E ratios were maintained in the same relative concentrations as determined in the striped bass fillets. Statistical analysis of weight gains, feed conversions and nitrogen balance indicated significant differences ( P  < 0.05) between treatments. Non-linear regression analysis of the response criteria pooled from both experiments yielded the following estimates of dietary EAA requirements (g kg–1 dry diet) when digestible energy equalled 13.39 MJ kg–1 diet: arginine, 14; histidine, 6; isoleucine, 9; leucine, 19; lysine, 22; methionine + cysteine, 10; phenylalanine + tyrosine, 17; threonine, 11; tryptophan, 3; and valine, 10. The use of fillet A/E ratios allows for the rapid estimation of quantitative EAA requirements and the development of species specific diets for new aquaculture species. The data presented here are the first to simultaneously describe all the dietary EAA requirements for M. saxatilis.  相似文献   

8.
A feeding trial was conducted to investigate the effects of essential amino acids (EAA) supplementation on the success of dietary protein reduction for Jian carp (Cyprinus carpio var. Jian). Five diets were formulated including a positive control (Diet 1, 378.4 g/kg protein), a negative control (Diet 5, 289.8 g/kg protein) and three test diets (Diet 2‐Diet 4, 340.4–299.0 g/kg protein supplemented with EAA). No significant decline of growth performance in fish fed decreased dietary protein with EAA supplementation, which were significantly higher than the negative control. The apparent digestibility coefficients (ADC) of protein and most EAAs were not significantly affected by the dietary treatments. Dietary treatments did not affect whole‐body composition, but influenced protein and EAAs retentions. Total ammonia nitrogen (TAN) excretion decreased with the decreasing dietary protein, and the highest plasma ammonia content was observed in fish fed Diet 5. Plasma glucose concentration increased with the decreasing dietary protein reduction, while other plasma parameters were not affected by dietary treatments. These results indicated that low‐protein diets (299.0 g/kg) supplemented with EAA can maintain protein, EAA digestibility and retention in Jian carp, and also has benefit in the reduction of TAN excretion.  相似文献   

9.
To determine the digestible lysine requirement for pacu juveniles, a dose–response feeding trial was carried out. The fish (8.66 ± 1.13 g) were fed six diets containing the digestible lysine levels: 6.8, 9.1, 11.4, 13.2, 16.1 and 19.6 g kg?1 dry diet. The gradual increase of dietary digestible lysine levels from 6.8 to 13.2 g kg?1 did not influence the average values of the parameters evaluated (P > 0.05). The increase of dietary digestible lysine level to 16.1 g kg?1 significantly improved weight gain (WG), specific growth rate (SGR), protein productive value (PPV), protein efficiency rate (PER), and apparent feed conversion rate (FCR), but was not different from fish fed diets containing 19.6 g kg?1 lysine. Fish fed diets containing 16.1 and 19.6 g kg?1 digestible lysine showed lower body lipid contents than fish in the other treatments. The digestible lysine requirement as determined by the broken‐line model, based on average WG values, was 16.4 g kg?1. The other essential amino acid requirements were estimated based on the ideal protein concept and the value determined for lysine.  相似文献   

10.
A study was conducted to evaluate the effect of dietary supplementation of lysine and/or methionine on growth performance, nitrogen retention and excretion in pacu juveniles reared in cages. Five diets were prepared; four diets based on plant ingredients containing 23% digestible protein (DP), basal: Lys- and Met-deficient, 23L: basal supplemented with lysine only, 23M: basal supplemented with methionine only, and 23LM: basal supplemented with both amino acids and a protein-bound AAs diet based on fish and soybean meal, containing 30% DP. Survival, specific growth rate, protein efficiency rate and feeding cost were not influenced by the dietary treatments (P > 0.05). Fish fed basal diet showed the lowest mean of N retention. Fish fed 30DP diet showed the best results of weight gain (WG) and apparent food conversion rate (FCR) among the dietary treatments. On the other hand, WG in the 23LM group and FCR in the 23LM and 23M groups were not significantly different from the group fed 30DP diet, and showed the highest mean of N retention. There was higher N excretion (P < 0.05) when the fish were fed 30DP and 23L diets than the other dietary treatments. Fish fed plant protein-based diets containing 23% DP supplemented with both amino acids or methionine alone showed satisfactory growth and N retention results when compared with fish fed 30DP diet, with the advantage of lower N emissions into water. The results also evidence the pacu's great potential to be reared in cages.  相似文献   

11.
The requirements of juvenile prawn, Marsupenaeus japonicus (Bate), 0.79 g initial body weight, for essential amino acids (EAA) were evaluated based on the daily increase of each EAA in the whole body when the prawn was maintained on a diet with a high nutritive value (a casein‐squid protein‐based diet). The quantities of each EAA needed daily for growth and maintenance of prawn are conceived to correspond to the daily requirements of this prawn species for EAA. Therefore, these requirement values of respective EAA should be supplied from dietary proteins. To determine these values, protein and amino acids of the whole body of the prawn were quantified before and after feeding experiments, and the quantities of respective EAA needed to meet the requirements were estimated based on the EAA profile of the whole body protein of prawn. As a result, the contents of EAA in dietary proteins (%) needed to meet the requirements of the prawn for EAA were assessed to be: threonine (2.3), methionine (1.3), valine (2.4), isoleucine (2.3), leucine (3.4), phenylalanine (2.6), lysine (3.2), histidine (1.1), arginine (2.9) and tryptophan (0.6), respectively, when the prawn are fed 50% protein diet with 90% protein digestibility at a ration size of 2% (% of body weight).  相似文献   

12.
Rapid methods of estimating dietary essential amino acid (EAA) requirements might facilitate increases in aquaculture production, particularly for new or emerging industries. We conducted a 12‐week feeding study to test the hypothesis that whole body EAA concentrations and the quantified methionine requirement could be used to predict the remaining dietary EAA requirements for juvenile all‐female yellow perch. Six purified diets were developed and fed to triplicate groups of fish for 12 weeks. The diets contained the EAA profile of fishmeal (FM), the profile as predicted by whole body analysis, the quantified methionine requirement and resulting A/E ratios (PRED), PRED + 20% of all EAA (PRED20), PRED + 40% of all EAA (PRED40), PRED + 20% threonine, isoleucine and tryptophan (PRED320), and PRED + 40% threonine, isoleucine and typtophan (PRED340). Mean weight gain and feed consumption were significantly higher in fish fed PRED20 (35.7 ± 3.2 and 55.0 ± 5.3 g, respectively) than in fish fed FSM (25.1 ± 0.4 g wt gain, 41.0 ± 1.9 g cons), PRED (23.4 ± 2.3 g wt gain, 40.1 ± 4.2 g cons) and PRED340 (22.9 ± 3.3 g wt gain, 35.0 ± 3.8 g cons). There was no significant difference in feed efficiency among treatments. We recommend an EAA profile similar to PRED20 for feeding all‐female juvenile yellow perch.  相似文献   

13.
A 12‐week feeding trial was conducted to estimate the effects of supplemented amino acids (lysine and methionine) with different dietary protein levels on growth, haematology and liver histology in meagre (Argyrosomus regius) at two different sizes: fingerling (15.2 g) and juvenile (44.4 g). Six practical diets were prepared, and three of these practical diets were formulated with 40 (P40), 44 (P44) and 48% (P48) crude protein without supplementation of amino acids. Other three diets having same protein levels were supplemented with lysine and methionine (P40AA, P44AA and P48AA) to bring these amino acids level up to that estimated to be at the least in the 48% protein diet. Final mean weight (FMW) and specific growth rate (SGR) of P48AA in fingerlings were higher than that of 40AA. According to FMW and SGR of juveniles, there was interaction found between dietary protein levels and the supplementation of amino acids. The FMW and SGR of meagre fed P44AA were higher than that of fish fed the other diets, except fish fed the P48. Economic profit index, packed cell volume and haemoglobin for juvenile meagre were significantly improved with the dietary protein level and amino acid supplementation. The liver tissues of the P48 group in both sizes displayed better structure compared with the other groups. In conclusion, dietary crude protein content of practical diets for juvenile meagre could be reduced from 48% to 44% by adding essential amino acids with an enhancement in growth, health and economic profit.  相似文献   

14.
This study evaluated the nutritional quality of farmed and wild axillary seabream (Pagellus acarne R., 1827) focusing on amino acid profiles, with regards to possible interactions with wild fish aggregating around the cage facility. Total amino acids (∑AA), essential amino acids (∑EAA), non‐essential amino acids (∑NEAA) and neutral amino acids (∑NAA) in farmed fish were lower than those in the wild individuals (p > .05). Amino acid pattern in cage‐aggregated fish showed a slight decline from the wild populations, but still higher than the farmed fish. Based on the amino acid scores (AAS), lysine and leucine could be underlined as the ‘first limiting amino acids’, but all other AASs were over ‘1’, in accordance with reference amino acid contents of FAO/WHO (>1.00), showing that farmed axillary seabream provides high nutritional quality and can be considered as a favourable protein source. The ratios of ∑EAA/∑AA (44%–46%) and ∑EAA/∑NEAA (79%–86%) exceeded the minimum recommendation of 40% and >60% by FAO/WHO for all three groups. It can be concluded that axillary seabream either farmed, farm‐aggregated or distant wild fish presented high‐quality protein generating a healthy source for human food.  相似文献   

15.
营养强化对灰海马幼体氨基酸组成的影响及品质的评价   总被引:2,自引:1,他引:1  
为评价复合营养型强化剂对灰海马(Hippocampus erectus)幼体氨基酸组成模式和营养品质的影响,用0.045 mL/L(A)、0.090 mL/L(B)和0.180 mL/L(C)3个浓度的50DE-微囊强化的卤虫投喂灰海马幼体。结果显示,灰海马氨基酸总量(TAA)的平均值为51.10%(质量分数,干样);灰海马含有17种氨基酸(AA),其中8种人体必需氨基酸总量(TEAA)占TAA比例的平均值为31.00%,4种呈味氨基酸总量(TDAA)占TAA的比例超过45.00%(质量分数,干样)。限制性氨基酸为异亮氨酸(Ile)和亮氨酸(Leu)。必需氨基酸指数(EAAI)的平均值为36.97。随着强化剂浓度升高,卤虫和灰海马体内部分AA、TAA、TDAA和非必需氨基酸(TNEAA)的含量及大多数单个必需氨基酸含量与必需氨基酸总量比值(A/E)均与强化剂浓度呈反比(P<0.05)。但是,卤虫和灰海马的TEAA/TAA和TEAA/TNEAA的值却随强化剂浓度升高而上升(P<0.05)。同时,从氨基酸评分(AAS)、化学评分(CS)和EAAI的评分模式可以看出,B、C两组灰海马的评分总体显著高于A组(P<0.05),但B、C两组间大部分氨基酸评分差异不显著(P>0.05)。结果说明,过高浓度的强化剂无法显著提高氨基酸的相对含量,但却能在一定程度上改善氨基酸的组成模式。  相似文献   

16.
ABSTRACT: Postprandial changes of free amino acid (FAA) concentrations in plasma and feed digesta contents were examined in yellowtail, Seriola quinqueradiata (220–280 g bodyweight) fed non-fishmeal diets either with or without supplemental crystalline amino acids (lysine, methionine, threonine, and tryptophan) in order to evaluate the availability of supplemental amino acids. Non-fishmeal diets containing 30% soy protein concentrate as the major protein source were prepared in three diet forms: soft dry pellet, extruded pellet, and single moist pellet. The level of plasma FAA and feed digesta content were determined at 0, 3, 6, 9, 12, 18, and 24 h after feeding. Plasma levels of four supplemental amino acids in fish fed the non-fishmeal diets with EAA were higher than those of fish fed diets without EAA, suggesting that yellowtail can efficiently absorb supplemental crystalline amino acids irrespective of diet form. However, a remarkable difference was observed in the periodical patterns of these four amino acids between FAA derived from supplements and those from feed protein. Moreover, FAA patterns in fish fed the non-fishmeal diets with EAA were different from those of the control fishmeal diet. Of note, methionine concentration was markedly high during the whole experimental period, resulting in an amino acid imbalance that may have caused lower feed performances in fish fed the non-fishmeal diets with EAA compared to the control fishmeal diet.  相似文献   

17.
One of the strategies used to improve fish production is lowering the feed costs and the environmental impact by reducing dietary protein content. Using the deletion method, we determined the optimal amino acid (AA) ratio for Nile tilapia (Oreochromis niloticus) (body weight 20 g). Eleven experimental diets and four replications, taken two at a time, distributed in a randomized block design were used. For this trial, a balanced diet (BD) was formulated. Ten other diets were formulated by the deletion method, in which the BD diet was adjusted to result in a reduction of 45% of the test amino acid. Fish were fed three times a day for 57 days. Groups of fish at the beginning and at the end of the experiment were euthanized for further determination of the carcass nitrogen (N) composition. The optimal ratio of each AA was derived by dividing the requirement for each AA by the requirements for lysine. The essential AA ratios, expressed relative to lysine (=100), were methionine 64, threonine 93, tryptophan 24, arginine 125, histidine 34, isoleucine 57, leucine 96, valine 76 and phenylalanine 101. Our findings might be used to design strategies aimed at reducing the production costs of Oreochromis niloticus.  相似文献   

18.
A feeding‐and‐digestibility trials were carried out to evaluate the efficacy of replacing fishmeal with brewers yeast Saccharomyces cerevisiae in diets of pacu, Piaractus mesopotamicus, juveniles. The feeding trial was conducted during 54 days with 450 fish (26.6 ± 1.7 g) testing six isonitrogenous (270 g kg?1 crude protein) and isoenergetic (19 MJ kg?1 crude energy) diets, with increasing yeast level to replace 0 (control), 30, 35, 50, 70 or 100% of dietary fishmeal. Growth performance and feed utilization increased with increasing dietary yeast level until 50% fishmeal replacement. Protein retention efficiency was higher in fish fed 35 and 50%. Protein digestibility and the fillet hue (the red/green chromaticity) were not significantly different among all treatments. Nitrogen gains were significantly improved in fish fed 35% replacement diet compared to fish fed the control diet. The retentions of indispensable amino acids tended to increase with increasing dietary yeast levels, with maximum retention at 35–50%. On the basis of our results, replacing 50% fishmeal by yeast in pacu diets successfully improved feed efficiency and growth performance, and reduced nitrogen losses, thereby reducing the nitrogen outputs from fish farms.  相似文献   

19.
A feeding trial was conducted to investigate the effects of different dietary amino acid patterns on growth performance, feed utilization and body composition of juvenile Nibea japonica. Four semi‐purified diets were formulated to simulate the dietary amino acid profiles of juvenile giant croaker whole body protein (GCP), Peru fishmeal protein (PFP), red sea bream eggs protein (REP) and soybean meal protein (SMP) by supplementing with pre‐coated crystalline amino acids (CAA). A control diet contained only intact protein sources provided by the fishmeal and casein (2:1). Each experimental diet was fed to satiated triplicate groups of juveniles (10.73 ± 0.07 g) twice a day for 8 weeks. The highest weight gain (WG) was observed in the juveniles fed the control diet, whereas no significant differences were found between the juveniles fed the GCP and control diets. Fish fed the control, GCP, PFP and SMP diets did not exhibit any significant difference in protein efficiency ratio (PER), feed conversion (FCR) or nitrogen (N) retention. The results of this study suggest that the amino acid (AA) patterns of juvenile whole body protein could be used as a guideline in the formulation of dry diets, which also confirms that the juvenile giant croaker is able to utilize high amounts (20%) of CAA in coated form for growth.  相似文献   

20.
Replacement of >25% of fish meal (FM) with aerobically converted carinata meal (ACCM) in low (200g/kg) animal protein (reference = 200g/kg FM) diets of rainbow trout Oncorhynchus mykiss resulted in reduced growth, partly due to reduced feed consumption and protein utilization. In this study, we determined the effect of FM replacement with ACCM on trypsin activity, apparent digestibility coefficients (ADCs) of protein, and essential amino acid (EAA) or conditionally essential amino acid (CEAA) ADCs and bioavailability. Replacement of FM did not alter protein ADCs or trypsin activity. Replacement of >25% FM reduced all EAA and CEAA ADCs except for arginine and leucine which were only reduced by 75% FM replacement. Only serum free lysine and muscle free histidine were reduced by >25% FM replacement. Muscle free lysine was only reduced by 75% FM replacement. Replacement of FM reduced EAA peak concentrations and resulted in slower release of EAAs in serum. Cumulative total EAAs in serum and muscle decreased with FM replacement. Ratios of EAAs to lysine showed that tryptophan was the most limiting EAA. However, isoleucine, leucine, methionine and phenylalanine were also inadequate for muscle synthesis for the first 9–12 hr following force‐feeding. Optimal time for protein synthesis was ≥36 hr. Although any level of FM replacement did not reduce protein ADCs and trypsin activity, replacement of ≥25% FM reduced EAA ADCs and bioavailability of lysine and histidine, which partly contributed to the observed differences in growth.  相似文献   

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