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1.
目的确定平贝母不同表现类型是否在总生物碱含量上存在差别,为其育种做准备。方法超声法提取总生物碱,比色法测定含量。结果平贝母常见的绿茎绿叶品系的总生物碱含量为0.1703%,另2种品系——紫茎紫叶、紫茎绿叶分别为0.1807%、0.1830%。结论不同平贝母表现类型在总生物碱含量上没在显著差异,需要转向对其他性状进行分析。  相似文献   

2.
目的优化丹参灵芝丸的提取工艺。方法:采用L_9(3~4)正交试验法,以丹参酮ⅡA和氧化苦参碱的含量的综合评分为评价指标,考察乙醇浓度、加醇倍量、提取时间及提取次数对脂溶性成分提取工艺的影响;以丹酚酸B和总多糖含量的综合评分为评价指标,考察加水倍量、提取时间、提取次数对水溶性成分提取工艺的影响,优化丹参灵芝丸的提取工艺。结果脂溶性成分最佳提取工艺为8倍量75%乙醇回流提取三次,每次1.0h;水溶性成分最佳提取工艺为8倍量水煎煮三次,每次1.5h。结论验证试验表明,该提取工艺稳定可行、重复性良好。研究为丹参灵芝丸工业化生产提供了依据。  相似文献   

3.
大豆异黄酮提取工艺的优化   总被引:1,自引:0,他引:1  
大豆异黄酮是一种从大豆种子中分离出的具有异黄酮类化合物结构的主要活性成分。广泛应用于食品及医药行业,传统的方法往往采用甲醇做溶剂提取,其具有毒性。试验分别使用食用乙醇和水进行分离提取,通过正交试验优化提取工艺,比较两种提取方法的效果。食用乙醇提取效果优于水提取的效果,比较适宜的提取条件为:采用80%乙醇,在提取温度为80℃,固液比为1:15,提取时间为3h,提取2次。提取率可高达0.47%。  相似文献   

4.
目的采用9种不同方法对天麻进行炮制,考察炮制前后天麻中天麻素、对羟基苯甲醇含量的变化情况及抗氧化活性的变化情况。方法采用HPLC法测定天麻生品和各炮制品中天麻素和对羟基苯甲醇的含量,并对不同炮制品进行DPPH法抗氧化实验。结果炮制后姜炙天麻中天麻素含量最高;炒黄后的天麻中天麻素含量最低;醋炙天麻中对羟基苯甲醇的含量最高;炒焦后的天麻中对羟基苯甲醇的含量最低;炒炭后的天麻抗氧化效果高于其他炮制品。结论不同炮制方法对天麻中两种化学成分的含量是有影响的,各炮制品的抗氧化活性也有差别。  相似文献   

5.
《中国茶叶》2007,29(6):46-46
绿茶的乙醇浸提液经浓缩、脱色和萃取,得茶多酚粗品,用聚酰胺层析柱分离提纯得(-)-EGCG单体。采用正交试验法考察了绿茶的粒度、乙醇用量和提取次数对(-)-EGCG提取率的影响,得出优化醇提工艺为:绿茶粉末粒径小于0.960mm,用12倍量的体积分数为95%的乙醇溶液回流提取3次,每次提取1h。研究了绿茶醇提液的活性炭脱色工艺,考察了活性炭用量、脱色次数、脱色时间对脱色液的吸光度和(-)-EGCG提取率的影响。  相似文献   

6.
不同产地平贝母营养元素、有效成分及其相关性研究   总被引:1,自引:0,他引:1  
为平贝母药材的质量评价提供参考和平贝母道地药材的规范化栽培提供理论指导,对东北三省9个不同产地的平贝母营养元素的含量和有效成分进行研究。结果显示平贝母中的N、P、K、B、Ca、Fe、Mg、Mn、Zn含量平均值分别为27420mg/kg、4440mg/kg、18500mg/kg、6.0mg/kg、1310mg/kg、610mg/kg、1160mg/kg、30mg/kg、160mg/kg,Cu、cd、Pb、Hg重金属含量不超标。9个不同产地之间的平贝母总生物碱含量差异达到了极显著水平;K、Hg和含量差异达到了显著水平,B、Ca、Fe、Mg、Mn、Zn、N、P、Cu、Cd、Pb和总皂苷含量的差异不显著。所有平贝母鳞茎营养元素和总生物碱含量无相关性,只有K元素和总皂苷含量呈显著正相关,可以模拟线性回归方程。  相似文献   

7.
【目的】探讨万寿菊中色素的最佳提取工艺。[方法】对万寿菊进行色素提取方案比较,确定最佳提取剂、料液比、浸提时间及浸提次数。【结果】色素在二元混合溶剂中提取率比单溶刺中高,较适宜的溶剂为石油醚:乙醇(3:2)的混合试剂;色素的最佳提取次数为3次,最适宜的料液比为1:15,每次最适宜的浸提时间为2.5h;万寿菊原料易得,色素提取工艺简单、能耗低、效率高、生产过程无污染。【结论】用石油醚:乙醇(3:2)作提取剂时提取万寿菊色素的效果最好,提取时的料液比为1:15,其提取次数为3次,时间为2.5h。  相似文献   

8.
目的优化大孔吸附树脂法纯化黄花草木樨叶中总皂苷的工艺。方法:以黄花草木樨中总皂苷的含量为指标,考察了5种大孔吸附树脂对黄花草木樨中总皂苷的吸附及解吸效果,筛选出最适皂苷纯化的树脂,并对纯化工艺进行了优化。结果:AB-8大孔吸附树脂适合纯化黄花草木樨中的总皂苷,最佳洗脱条件为:上样浓度为1.0mg/ml,上柱吸附流速为2.0ml·min^-1,以70%乙醇洗脱,洗脱流速2.0ml·min^-1,洗脱体积为5BV。结论所选工艺方法简便,稳定,纯化效果好。  相似文献   

9.
涂建飞  卢立  张晶 《人参研究》2013,25(2):43-45
目的测定菜豆豆荚总黄酮含量,优化菜豆中黄酮提取工艺。方法以芦丁为对照品通过紫外分光光度法测定总黄酮含量,将回流法、超声法、煎煮法提取黄酮类化合物的提取效率进行对比,采用正交法优化了超声提取工艺。用最佳提取工艺验证实验结果。结果提取次数对黄酮提取率影响显著.超声时间和乙醇浓度对提取率影响不显著。结论最佳提取工艺为85%乙醇、超声60min、提取3次,菜豆中总黄酮含量为158.86mg/100g。  相似文献   

10.
目的 优选人参药性菌质中总皂苷的最佳提取工艺条件.方法 通过单因素考察、正交试验考察,确定最佳工艺条件.以总皂苷含量为指标,采用紫外可见分光光度法测定.结果 人参药性菌质最佳提取条件为加7倍量50%乙醇,回流提取2次,每次1.5小时.结论 优选出的提取工艺简单,效率高,成本较低,可适用于大生产.  相似文献   

11.
The seeds ofLupinus mutabilis show on average a protein content of 42.6% and a fat content of 18.7%. Unfortunately they contain up to 4.5% of bitter alkaloids which must be removed before consumption. Edible oil and protein concentrate were produced from the seeds at an industrial level. The oil is of good quality and is recommended for human nutrition. The main fatty acid is oleic while the main tocopherol is γ-tocopherol. During the refining process, which includes a washing process with aqueous acids for debittering, the alkaloid content of the oil is reduced to 5 ppm. During the extraction of the oil the alkaloids are concentrated in the oil cake, from which they can be removed by washing with an ethanol-water mixture. A protein concentrate containing 72.3% protein and 0.06% residual alkaloids is obtained. The analysis of alkaloid pattern by gas-chromatography showed that the alkaloid composition of the crude oil differs totally from that of the seed. The relative percentage of the alkaloid esters and that of the Tetrahydrorhombifoline are very much higher in the oil than in the seed. During the debittering process of the oil cake the relative percentage of the hydroxylupanines increases.  相似文献   

12.
In industrial plants such as medicinal plants, the content of the economically important metabolite is more important than the yield of the plant part containing the metabolite, as it determines the cost of extraction of the metabolite. Two high alkaloid content mutants of periwinkle (Catharanthus roseus, a medicinal plant yielding anti-cancer and anti-hypertension alkaloids) were developed through induced mutagenesis. Since the availability of nitrogen (N) is expected to play an important role in the biosynthesis and accumulation of alkaloids in plants, the effect of N fertilization on the content of alkaloids of two high alkaloid content mutants of periwinkle was studied, in comparison with their parental variety, Nirmal, to determine the possibility of further increasing their alkaloid contents. The three genotypes were evaluated at three levels of N (0, 100 and 150 kg ha−1) fertilization in a split plot experiment, which was repeated over 2 years. N fertilization significantly increased the content of alkaloids both in leaves and roots of all genotypes. Over genotypes, application of 150 kg ha−1 of N resulted in an increase of 42 and 32% in the content of leaf and root alkaloids, respectively. However, the increase was highest in genotypes with the lowest content of leaf or root alkaloids. The high alkaloid mutants grown at 150 kg ha−1of N fertilization exhibited an increase of 87 and 56% in the content of leaf and root alkaloids, respectively, when compared with their parental variety grown without nitrogen fertilization. N fertilization increased leaf and root yields by 170 and 90%, respectively. The increase in contents of leaf and root alkaloids, due to N application, paralleled the increase in leaf and root yields.  相似文献   

13.
Since ancient times, ginger (Zingiber officinale) has been widely used for culinary and medicinal purposes. This rhizome possesses several chemical constituents; most of them present antioxidant capacity due mainly to the presence of phenolic compounds. Thus, the physical conditions for the optimal extraction of antioxidant components of ginger were investigated by applying a Box-Behnken experimental design. Extracts of ginger were prepared using water as solvent in a conventional solid–liquid extraction. The analyzed variables were time (5, 15 and 25 min), temperature (20, 55 and 90 °C) and sample concentration (2, 6 and 10 %). The antioxidant activity was measured using the 2,2-diphenyl-1-picrylhydrazyl method and a modified ferric reducing antioxidant power assay while total phenolics were measured by Folin & Ciocalteu’s method. The suggested experimental design allowed the acquisition of aqueous extracts of ginger with diverse antioxidant activity (100–555 mg Trolox/100 g, 147–1237 mg Fe2+/100 g and 50–332 mg gallic acid/100 g). Temperature was the determining factor in the extraction of components with antioxidant activity, regardless of time and sample quantity. The optimal physical conditions that allowed the highest antioxidant activity were: 90 °C, 15 min and 2 % of the sample. The correlation value between the antioxidant activity by ferric reducing antioxidant power assay and the content of total phenolics was R2?=?0.83. The experimental design applied allowed the determination of the physical conditions under which ginger aqueous extracts liberate compounds with antioxidant activity. Most of them are of the phenolic type as it was demonstrated through the correlation established between different methods used to measure antioxidant capacity.  相似文献   

14.
目的为了解市售人参、西洋参制品糖分含量特性,方法采用比色法对60个人参、西洋参样品总糖和还原糖含量进行测定。结果蜜制参类制品总糖含量显著高于其它类制品,红参、生晒参、鲜参、保鲜参和活性参间总糖含量差异不显著。生晒参、鲜参、保鲜参和活性参还原糖含量小于6%,红参还原糖含量不高于16%,蜜制参还原糖含量均大于24%。结论还原糖含量应作为人参、西洋参制品质量的评价指标。  相似文献   

15.
林小苹  赖钟雄 《热带作物学报》2015,36(10):1796-1801
以铁皮石斛的原球茎、无根苗(无根试管苗)、茎段和叶片为材料,研究不同光质条件对铁皮石斛原球茎增殖及4种材料中多糖和生物碱含量的影响。结果表明,在绿光下原球茎的增长率明显低于其他光质,在白光和蓝光下原球茎的增长率较好;在4种材料中,蓝光能促进生物碱的合成,有利于生物碱积累,而红光能促进碳水化合物的积累,有利于多糖含量增加,绿光则不同程度地抑制多糖和生物碱的积累。  相似文献   

16.
以13份广东省境内收集的苦茶资源为研究对象,来自不同省份的28份代表性品种(系)为对照,分析苦茶资源中嘌呤生物碱含量特征.结果显示,咖啡碱、可可碱、苦茶碱及生物碱总量均表现出较大的遗传差异,遗传多样性指数(H′)为0.78~1.94,变异系数(CV)为34.89%~191.19%.系统聚类分析将试验材料分为6个类群,类...  相似文献   

17.
Five varieties (‘Minoan’, ‘Medea’, ‘Korona’, ‘Przemko’, ‘Kozmosz’) of poppy representing different chemotypes were combined and the alkaloid profiles of F1-F3 progenies were studied during 2006-2009.In the crosses of high alkaloid containing varieties the content of total alkaloids and that of morphine and thebaine showed increased levels in the hybrid generations which persisted till F3. Narcotine (noscapine), however accumulated at lower level than the midparent values and showed a decreasing tendency over generations. A higher number of homogenous strains starts to appear in F3 progenies. The majority of codeine containing individuals concentrates to certain strains. A growing number of high thebaine containing individuals was observed in several combinations. In the crosses with low alkaloid containing parent (‘Przemko’) the F1 exhibits considerable heterosis for total alkaloid content. Low alkaloid containing recessive individuals segregate in F2 and stabilise in F3.Results of our crossing experiments reflected well the effects of genetic regulation at three levels of enzymatic processes during the biosynthesis of poppy alkaloids. Data support the recessive determination of transformations at TyDC (tyrosine decarboxylase) and BBE (berberine bridge enzyme) levels while more complex (polygenic) effects are supposed in controlling the quantity of narcotine (noscapine) and morphinanes. Selection for fixing very low alkaloid content and narcotine may be effective in early F2 generations, however a selection for morphinanes is not worthy before F3.  相似文献   

18.
为降低红曲黄酒的热性,在传统红曲黄酒酿造工艺基础上引入绿茶共发酵技术,研究其对红曲黄酒酿造品质及寒热性的影响。结果表明,在落缸时添加绿茶共发酵可丰富红曲黄酒的风味,抑制红曲黄酒的酸化,降低红曲黄酒的热性。绿茶的适宜添加量为1.0‰,发酵后的黄酒出酒率为传统黄酒的1.12倍,总酸含量降低9.65%,总酚含量提高13.3%,寒热指数下降43.09%,差异均达极显著水平(P0.01)。酿造的红曲黄酒新产品物性温和、口感酸涩适中、酒香茶香协调,且不失红曲黄酒典型性风格。该研究为研发温和型红曲黄酒新产品提供了理论依据和技术支持。  相似文献   

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