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1.
Influence of NPK on performance of the stem-nodulating legumes Sesbania rostrata and Aeschynomene afraspera in lowland rice The stem-nodulating tropical legumes S. rostrata and A. afraspera are promising green manure species for the low-input rice farming systems of lowland areas. Nutrient imbalances and soils low in available nutrients can considerably affect the use of biofertilizers. Use of mineral N, P, and K fertilizers in the growth of S. rostrata and A. afraspera as biofertilizers for lowland rice in the Philippines was evaluated. Applied P and K both stimulated growth, nodulation, and N2 fixation. N accumulation in PK fertilized S. rostrata was about 40% higher than in nonfertilized green manure. Mineral N application (urea) depressed nodulation and N2 fixation (ARA) in roots and resulted in an increased ARA in stem nodules. The legumes produced more N gains in the presence of small amounts of N. Use of S. rostrata and A. afraspera green manure increased in all cases significantly rice grain yield. Thus integration of mineral N, P, and K fertilizers in a green manurebased rice farming system can considerably improve biofertilizer production and increase rice grain yield.  相似文献   

2.
The performance of Sesbania rostrata varies widely from site to site. This makes it difficult to predict the N yield and biomass of this plant in marginally productive soils, and to arouse the interest of farmers in green manure technology. Three consecutive pot experiments were conducted in a greenhouse at the International Rice Research Institute (IRRI) to evaluate growth, nodulation, N2 fixation (C2H2 reduction assay and 15N dilution method), and N yield of 6-week-old S. rostrata on 13 physicochemically different wetland rice soils of the Philippines and on three artificial substrates. The performance of S. rostrata on the unfertilized controls was compared with two fertilizer treatments containing either P (100 mg P kg-1 dry soil) or P+K (100 mg P kg-1 and 200 mg K kg-1 dry soil). In the control soils and substrates, the N yield of S. rostrata varied between 20 and 470 mg N per pot, with the N rate from N2 fixation ranging between 0 and 95%. In three of the nutritionally poor soils even Mn toxicity symptoms apparently occurred with S. rostrata. P application alleviated these symptoms and increased the overall N yield considerably, mainly through increased biological N2 fixation. An additional increase in N yield was obtained by the PK treatment. Multiple regression analysis between soil characteristics and the N yield of S. rostrata showed that the original level of P (Olsen-extracted) and Mn in the soil accounted for 73% of the variance in biomass production by S. rostrata among the unfertilized soils and substrates.  相似文献   

3.
ABSTRACT

Biological nitrogen fixation (BNF) is an important nitrogen source for both N2-fixers and their neighboring plants in natural and managed ecosystems. Biological N fixation can vary considerably depending on soil conditions, yet there is a lack of knowledge on the impact of varying soils on the contribution of N from N2-fixers in mixed swards. In this study, the amount and proportion of BNF from red clover were assessed using three grassland soils. Three soil samples, Hallsworth (HH), Crediton (CN), and Halstow (HW) series, were collected from three grassland sites in Devon, UK. A pot experiment with 15N natural abundance was conducted to estimate BNF from red clover, and the proportion of N transferred from red clover to the non-N2 fixing grass in a grass-clover system. The results showed that BNF in red clover sourced from atmosphere in the HH soil was 2.92 mg N plant?1, which was significantly lower than that of the CN (6.18 mg N plant?1) and HW (8.01 mg N plant?1) soils. Nitrogen in grass sourced from BNF via belowground was 0.46 mg N plant?1 in the HH soil, which was significantly greater than that in CN and HW soils. However, proportionally there were no significant differences in the percentage N content of both red clover and grass sourced from BNF via belowground among soils, at 65%, 67%, 65% and 35%, 27%, 31% in HH, CN, and HW, respectively. Our observations indicate that the amount of BNF by red clover varies among grassland soils, as does the amount of N sourced from BNF that is transferred to neighboring plants, which is linked to biomass production. Proportionally there was no difference among soils in N sourced from BNF in both the red clover plants and transferred to neighboring plants.  相似文献   

4.
In cowpea, efficient N2‐fixing genotypes are being selected to promote sustainable cropping systems in southern Cameroon (SC). However, N2 fixation and growth of these genotypes are largely hampered by low levels of soil plant‐available P. To evaluate the genotypic variation in N2 fixation and P uptake among cowpea (Vigna unguiculata L.) genotypes, field experiments were conducted over two years on two acid soils low in available P. The experiments were laid out in a split‐block design with four replications on typic (TK) and rhodic (RK) Kandiudult soils with seven cowpea genotypes. Phosphorus (P) fertilizers were applied on the main plots with 0 kg P, 30 kg P ha–1 as triple superphosphate (TSP) and 90 kg P ha–1 as Togo phosphate rock (PR). Nodule dry matter (DM), shoot DM, grain yield, and P uptake of cowpea significantly varied with site, P application, and genotype (p < 0.05). The N2 fixation of the cowpea genotypes ranged from 29 to 51 kg N ha–1 on both TK and RK soils and was significantly increased with P application. Significant genotypic variations in N2 fixation were observed with superior ability of the genotypes IT89KD‐391 and IT90K‐59 to fix N2. The harvest index (HI) did not significantly differ between soils and P application levels (p > 0.05). Four genotypes were selected to investigate root mechanisms responsible for efficient P acquisition in pot experiments. The results suggest that a better root infection by arbuscular mycorrhizal fungi (AMF) in genotype IT90K‐59 and root morphological and physiological characteristics in IT89KD‐391 were the most important factors for increasing P uptake.  相似文献   

5.
Rising carbon dioxide (CO2) concentration causes fertilization effects resulting in enhanced crop biomass and yields and thus likely enhances nutrient demand of plants. Hence, this field study was carried out to investigate the effects of elevated CO2 and N on biomass yield, nutrient partitioning, and uptake of major nutrients by soybean (Glycine max L.) using open‐top chambers (OTCs) of 4 m × 4 m size. Soybean was grown in OTCs under two CO2 [ambient and elevated (535 ± 36.9 mg L?1)] and four N levels during July to October 2016. The four N levels were N0, N50, N100, and N150 referring to 0, 50, 100, and 150% recommended dose of N. Both CO2 and N significantly affected biomass and grain yield, though the interaction was non‐significant. CO2 enrichment produced 30–65% higher biomass and 26–59% higher grain yield under various N levels. As compared to the optimum N application (N100), the CO2‐mediated increment in biomass yield decreased with either lower or higher N application, with the response being lowest at N150. As compared to ambient concentration, elevated CO2 resulted in significant reduction of seed P concentration at all N application levels but at N150, an opposite trend was observed. The decrease in seed P was maximum at N0 and N50 (7–9%) and by 3% at N100, whereas there was a gain of 7.5% at N150. The seed N and K concentrations were not affected either by CO2 or N application. Total N, P, and K uptake at harvest were significantly affected by CO2 and N, but not by CO2 × N interaction. Elevated CO2 resulted higher uptake of N by 18–61%, P by 23–62%, and K by 22–62% under various N treatments.  相似文献   

6.
长期施肥对盐渍化土壤肥力的影响   总被引：3，自引：0，他引：3  
The long-term effects of annual fertilizer applications on the fertility of salt-affected soils under the rotation system of wheat （Triticum aestivum L.） and maize （Zea mays L.） are not well documented. In 1984, research plots were established to test the effects of annual applications of different rates of nitrogen （N） and phosphorus （P） fertilizers on the fertility of a salt-affected soil （Typic Ustochrept） at the Quzhou Experimental Station, Quzhou County, Hebei Province, China. In October 2001, composite soil samples （0-20 and 20 40 cm） were collected from each plot and analyzed for soil fertility indices. Seventeen years of N and P fertilizer applications increased the soil organic matter （SOM） in the surface layer. With combined N, 270 （N1） and 540 （N2） kg N ha^-1 year^-1, and P, 67.5 （P1） and 135 （P2） kg P205 ha^-1 year^-1, fertilizer applications, total soil N mostly significantly decreased （P〈0.05）. Soil total P in the 0-20 cm layer of the P2 treatment significantly （P （0.05） increased as compared to those of the other treatments. Rapidly available P （RP） in the 0-20 cm layer of the N1P2 treatment was significantly higher than those in the other treatments except the P2 treatment; and RP in the 0-20 cm layer of the P2 treatment significantly increased as compared to those of the other treatments except the P1 and N1P2 treatments. RP in the subsurface soil layer （20-40 cm） of the P2 treatment （4.2 mg P kg^-1） was significantly （P〈0.05） higher than those in the other treatments. Nevertheless, long-term N fertilization did not significantly increase the alkali-hydrolyzable N in the soil. However, in the salt-affected surface soils the application of combined N and P fertilizers over 17 years significantly （P〈0.05） decreased rapidly available potassium （K）. The results suggested that while under long-term fertilizer applications some soil fertility parameters could be maintained or enhanced, careful monitoring of soil fertility was necessary as other nutrients such as K could become depleted.  相似文献   

7.
A recycling of Phosphorus (P) from the human food chain is mandatory to secure the future P supply for food production. However, many available recycled P fertilizers from sewage sludge do not have an adequate P bioavailability and, thus, are not suitable for their application in soils with pH >5.5–6.0, unless being combined with efficient mobilization measures. The aim of the study was to test the P mobilization ability of red clover (Trifolium pratense L.) from two thermally recycled P fertilizers for a subsequently grown maize. Two sewage sludge ashes (SSA) were investigated in a pot experiment at soil pH 7.5 with red clover differing in its nitrogen (N) supply (added N fertilizer or biological N2 fixation (BNF)), followed by maize (Zea maize L.). Shoot dry matter of maize was almost doubled when N supply of previous grown clover was covered by BNF, instead of receiving added N fertilizer. Similarly, shoot P removal of maize following clover with BNF was significantly increased. It is suggested that the P mobilization is related to the BNF, and a proton release of N2 fixing clover roots led to the measured decrease in soil pH and thereby increased P availability of the tested fertilizers.  相似文献   

8.
A greenhouse experiment was conducted to investigate the effect of a P application (0 vs. 50 mg P kg-1) on yield, nodulation, and N2 fixation by three cowpea cultivars (Soronko, Amantin, and IT81D-1137) using the 15N isotope-dilution method. When P was not applied the inoculated cowpea genotypes showed significant differences (Soronko>Amantin> IT81D-1137) in N accumulation, in contrast to the uninoculated cowpea cultivars, which accumulated similar amounts of N. The differences in shoot N in inoculated plants were thus caused by differences in N2 fixation. The average values of N fixed (for both P levels) were 74% in Soronko, 59% in Amantin, and 42% in IT81D-1137, corresponding to 80, 51, and 24 mg N plant-1, respectively. Inoculation increased the total shoot-N accumulation in cv. Soronko by 270% without P and by 204% with P, cv. Amantin by 152 and 104%, and cv. IT81D-1137 by 74 and 58%, respectively. With P, the % N derived from atmosphere (%Ndfa) was 42% for IT81D-1137, 62% for Amantin, and 76% for Soronko. The high value for Soronko indicates that in a soil of medium fertility, certain cowpea cultivars are capable of satisfying their total N requirement through N2 fixation. The P effect on N2 fixation was mainly in the total amount of N fixed rather than on the percentage derived from the atmosphere.  相似文献   

9.
Six phosphorus (P) fertilizers recycled from sewage sludge [Struvite SSL, Struvite AirPrex®, P‐RoC®, Mephrec®, Pyrolysis coal and Ash (Mg‐SSA)] were tested for their plant availability in potted soil of pH 7.2 under greenhouse conditions. The crop sequence simulated a rotation of red clover (Trifolium pratense L.), maize (Zea maize L.), and ryegrass (Lolium perenne L.). Other P fertilizer treatments included: Phosphate Rock (PR), Calcium dihydrogen phosphate [Ca(H2PO4)2], and an unfertilized control. Additionally, soil was regularly inoculated with two strains of plant growth‐promoting rhizobacteria (PGPR; Pseudomonas sp. Proradix, and Bacillus amyloliquefaciens) to test their ability to increase P availability to plants. Sequential P fractionation was conducted to link the amount of readily available P in fertilizers to plant P acquisition. Shoot P content and dry matter of maize decreased in the following order: Struvite SSL ≥ Ca(H2PO4)2 > P‐RoC® ≥ Struvite AirPrex® ≥ Mephrec® > Pyrolysis coal ≥ Mg‐SSA ≥ PR ≥ unfertilized. Rhizobacteria did not affect shoot biomass or P content. The results show that red clover might have mobilized substantial amounts of P. Sequential P fractionation was not suitable to predict the efficacy of the fertilizers. Generally, the sewage sludge‐based fertilizers tested proved to be suitable alternative P sources relevant to organic farming systems. However, the efficacy of recycled fertilizers is strongly dependent on their specific production conditions.  相似文献   

10.
Influence of different fertilization on pH, N, C and CAL-extractable K and P in the soil The influence of different fertilization (mineral fertilizer, different kinds and quantities of farmyard manure and biocompost, horn meal) on soil properties was studied during 8 years of cultivation. The plots were planted and harvested as practised under local farming conditions. The pH of the non-fertilized plots decreased from 5.84 to 5.69, while it was increased by fertilization with farmyard manure or biocompost. Nt in the soil was not influenced by different fertilization. Ct was increased by high biocompost application, stayed constant on the other fertilized plots but decreased on the nonfertilized plots from 1.08 to 0.99%. Without fertilization, plant available nutrients were diminished from 7.3 to 4.3 mg P(CAL) 100 g?1 and from 22.5 to 13.9 mg K(CAL) 100 g?1. However, if the plots were fertilized with mineral or organic fertilizer, the nutrient content remained on the initial level. Storing farmyard manure under roof or covering with straw or polyethylene sheet did not affect the criteria of soil studied.  相似文献   

11.
There have been few long-term field studies on greenhouse gases measurement in organic crop rotations under temperate climatic conditions. Little is known about the extent to which the share of legumes in a crop rotation of organic farming affects the potentials for CO2 emission and soil organic carbon sequestration. The current study was aimed to investigate soil physicochemical state and soil net CO2 exchange rate in diverse organic crop rotations with different crop species and proportions of legumes. Four 5-year duration crop rotations were investigated. The best soil sustainability of the arable layer was found in a crop rotation enriched with red clover (Trifolium pratense L.). This rotation resulted in the highest soil mesoporosity and the lowest microporosity, ensured the best supply of plant-available water and revealed high soil resistance to dry conditions. Red clover secured the highest soil organic C sequestration, caused the increase in reserves of total N and available K, and slackened the decrease of soil-available P sources. Red clover-based cropping system exhibited the highest soil net CO2 exchange rate during five experimental years. The effect of crop rotation, consisting of phacelia (Phacelia tanacetifolia Benth.), peas (Pisum sativum L.) and yellow lupin (Lupinus luteus L.), on soil sustainability was weaker than the effect of rotation with red clover. Non-legume rotations, i.e. binary (two-crop) rotation and the crop rotation involving four spring and one winter species, can be regarded as miners of soil nutrient resources rather than contributors. These rotations did not promote soil sustainability because the soil lost large amounts of macronutrients and caused 26–33% lower soil net CO2 exchange rate, compared with leguminous rotations. For future, it could be recommended for ecological farming to rely more on crop rotations with red clover to improve ecosystems functioning.  相似文献   

12.
Abstract

An irrigated field trial was conducted to test the effects of white clover in three turfgrass species (perennial ryegrass, Kentucky bluegrass, and creeping bentgrass) on color, clipping yield, and botanical composition and to estimate nitrogen (N)2 fixation and N transfer from white clover to associated turfgrass species under different N‐fertilization conditions in 1999–2002.

Nitrogen fertilizers significantly increased color ratings in all observations. Grass–white clover mixtures had better color ratings than pure grass at all sampling dates and seasonal averages in unfertilized conditions. Fertilized pure grass plots yielded significantly more than control plots in all turfgrass species. Nitrogen fertilization did not affect clipping yield greatly in turfgrass–white clover mixtures. Nitrogen application significantly decreased white clover percentage in the harvested clippings in second and third year.

Nitrogen fertilization increased tissue N concentration positively in all turfgrass species grown alone. In contrast, N fertilization did not greatly affect tissue N concentration of either turfgrass species or white clover in the mixtures. Nitrogen fixation of white clover was estimated as 24.6, 30.7, and 33.8 g m?2 year?1 in perennial ryegrass, Kentucky bluegrass, and creeping bentgrass, respectively. The total estimated N2 fixation gradually decreased with increasing N fertilization. Nitrogen transfer from white clover to the associated turfgrass varied from 4.2 to 13.7% of the total N that the white clover fixed annually.  相似文献   

13.
Abstract

In a previous study, the understorey biomass production and biological nitrogen (N) fixation of a grassed‐down organic apple orchard were presented. The aim of this paper is to report the results of a similar study of two conventional orchards in a nearby location and to compare the present results with those of the organic orchard. Biological N2 fixation was determined in the field using the 15N isotopic dilution technique and the experiments were conducted over a two‐year period. Present results showed that substantial amounts of N (112 to 143 kg N ha‐1.2 years‐1) were fixed in the understorey of the conventional orchard and these were not significantly different from those of the organic orchard. However, the N2 fixation was sustained in the conventional, but not in the organic orchards in the second year, probably due to regular additions of fertilizers in the conventional orchards. In both orchards, N2 fixation was better correlated with clover than total dry matter yield. Seasonal effects found were highest N2 fixation and biomass production occurring during late spring and early summer and lowest during winter. Climatic factors were investigated in one of the conventional orchards and it was found that seasonal effects were related to a combination of temperature and moisture deficit effects.  相似文献   

14.
通过盆栽试验,研究施肥对喀斯特地区植草土壤不同活性有机碳组分和牧草固碳的影响。试验处理包括CK（不施肥）、 N1 （N 150 mg/kg）、 N2 （N 250 mg/kg）、 N1P1 （P2O5100 mg/kg）、 N2P2 （P2O5150 mg/kg）、 N1P1K1 （K2O 70 mg/kg）、 N1P1K2 （K2O 105 mg/kg）和N2P2K1和N2P2K2。结果表明,与对照（不施肥）相比,施肥处理增加植草土壤有机碳、 微生物量碳和易氧化碳,有机碳日矿化量和累积矿化量以及牧草固碳量。其中N1P1K1处理土壤有机碳和易氧化碳最高,N1P1处理土壤微生物量碳最高,N2P2K1处理土壤可溶性碳最高,N2P2K2处理牧草地上部及根系固碳量、 有机碳日矿化量和累积矿化量均最高。综上,低量氮磷钾肥配施有利于土壤活性有机碳的积累,高量氮磷钾平衡配施牧草固碳效果最佳。  相似文献   

15.
Leguminous leys are important sources of nitrogen (N), especially in forage-based animal production and organic cropping. Models for estimating total N2 fixation of leys—including below-ground plant-derived N (BGN)—are based on grazed or harvested leys. However, green manure leys can have different proportions of above-ground plant-derived N (AGN) and BGN when subjected to different cutting regimes. To investigate the effects of cutting on N distribution in white clover, a pot experiment was carried out using 15N techniques to determine N2 fixation, N rhizodeposition and root C and N content of cut and uncut white clover (Trifolium repens L. cv. Ramona) plants. Percentage N derived from air (%Ndfa) was lower in uncut (63%) than in cut (72%) plants, but total Ndfa was not significantly affected by cutting. The higher reliance on N2 fixation in cut plants was thus counterbalanced by lower biomass and total N content. With BGN taken into account, total plant-derived N increased by approximately 50% compared with AGN only. Cutting did not affect the proportion of BGN to standing shoot biomass N after regrowth, but decreased the proportion of BGN to total shoot biomass production during the entire growth period. Thus, estimates of N fixation in green manure leys should consider management practices such as cutting regime, as this can result in differences in above- and below-ground proportions of plant-derived N.  相似文献   

16.
Low soil fertility and soil acidity are among the major bottlenecks that limit agricultural productivity in the humid tropics. Soil management systems that enhance soil fertility and biological cycling of nutrients are crucial to sustain soil productivity. This study was, therefore, conducted to determine the effects of coffee‐husk biochar (0, 2.7, 5.4, and 16.2 g biochar kg?1 soil), rhizobium inoculation (with and without), and P fertilizer application (0 and 9 mg P kg?1 soil) on arbuscular mycorrhyzal fungi (AMF) root colonization, yield, P accumulation, and N2 fixation of soybean [Glycine max (L.) Merrill cv. Clark 63‐K] grown in a tropical Nitisol in Ethiopia. ANOVA showed that integrated application of biochar and P fertilizer significantly improved soil chemical properties, P accumulation, and seed yield. Compared to the seed yield of the control (without inoculation, P, and biochar), inoculation, together with 9 and 16.2 g biochar kg?1 soil gave more than two‐fold increment of seed yield and the highest total P accumulation (4.5 g plant?1). However, the highest AMF root colonization (80%) was obtained at 16.2 g biochar kg?1 soil without P and declined with application of 9 mg P kg?1 soil. The highest total N content (4.2 g plant?1) and N2 fixed (4.6 g plant?1) were obtained with inoculation, 9 mg P kg?1, and 16.2 g biochar kg?1 soil. However, the highest %N derived from the atmosphere (%Ndfa) (> 98%) did not significantly change between 5.4 and 16.2 g kg?1 soil biochar treatments at each level of inoculation and P addition. The improved soil chemical properties, seed yield, P accumulation and N2 fixation through combined use of biochar and P fertilizer suggest the importance of integrated use of biochar with P fertilizer to ensure that soybean crops are adequately supplied with P for nodulation and N2‐fixation in tropical acid soils for sustainable soybean production in the long term.  相似文献   

17.
To compare the growth performance of Brassica in a phosphorus (P) stress environment and response to added P, six Brassica cultivars were grown in pots for 49 days after sowing, using a soil low in P [sodium bicarbonate (NaHCO3)–extractable P = 3.97 mg kg?1, Mehlich III–extractable P = 6.13 mg kg?1] with (+P = 60 mg P kg?1 soil) or without P addition (0P). Phosphorus‐stress markedly reduced biomass accumulation and P uptake by roots and shoots. However, root–shoot ratio remained unaffected, implying that relative partitioning of biomass into roots and shoots had little role to play in shoot dry matter (SDM) production by cultivars. Biomass correlated significantly (P < 0.01) with total P uptake. Under P stress, the cultivars that produced greater root biomass were able to accumulate more total P content (r = 0.95**), which in turn was related positively to SDM and total biomass (r > 0.89**) and negatively to P‐stress factor (r = ?0.91**). There was no correlation between P efficiency (PE) (relative shoot growth) and plant P, but PE showed a very significant correlation with shoot P content and SDM. Wide differences in growth and better performance of cultivars such as ‘Brown Raya’ and ‘Con‐1’ under P stress encouraged screening of more germplasm, especially in the field, to identify P‐tolerant cultivars.

In another study, potential relative agronomic effectiveness (RAE) of sparingly soluble P sources was investigated by growing two contrasting cultivars. The P sources incorporated into soil at 0, 10, 25, 50, and 100 mg P Kg?1 were (i) powdered Jordan rock P (RP), (ii) triple superphosphate (TSP), (iii) powdered low‐grade TSP [TSP(PLG)], (iv) a mixture of RP + TSP compacted into pellets at 50:50 P ratio [RP + TSP(PelC)], and (v) a mixture of powdered RP + TSP at 50:50 P ratio [RP + TSP(PM)]. The RP was low in RAE and only 5 and 29% as effective as TSP in producing dry matter (DM) of P‐sensitive ‘B.S.A.’ and P‐tolerant ‘Brown Raya’ cultivars, respectively. There were no significant differences between TSP and RP + TSP(PelC) in DM yield of ‘Brown Raya,’ whereas, in the case of ‘B.S.A.’ RP + TSP(PM) was significantly less effective than RP + TSP(PelC) compared with TSP. Combined utilization of superior genome and P sources [such as TSP(PLG) and RP + TSP(PelC)] produced from low‐grade RP (that cannot be used either for direct application or acidulated P fertilizers) can be used as an alternative strategy for sustainable crop production, especially in resource‐poor environments. Further field trials at the level of cropping systems are needed.  相似文献   

18.
The effects of soil mesofauna and different farming systems on decomposition of clover (Trifolium repens) litter were investigated in a laboratory experiment. Microcosms were incubated for 16 weeks with fine and coarse litterbags in soils from three types of management systems: fallow, integrated farming and organic farming, the latter two cropped with wheat. The effects were studied by analysing litter mass loss, C and N content, DOC, nitrate and pH in soil leachate, and CO2 production, as well as mesofauna. Mesofauna significantly accelerated mass loss and C and N release from clover litter in all three soils. With mesofauna access, at the end of the experiment average clover mass loss was almost twice as high and clover C and N content were 60% lower than without mesofauna. Farming systems influenced the decomposition through affecting both element turnover and mesofauna. Although in the first weeks less N was leached from organic farming than from integrated farming soil, cumulative N leaching did not differ between these soils. However, more than 20% less N was leached from the fallow soil than from the field soils. CO2 production was highest in fallow soil. Here, mesofauna had no effect on this variable. In soil with integrated farming, mesofauna reduced cumulative CO2 production by 10% whereas in soil from organic farming it increased CO2 production by 20%. Our data suggest that differences in C and N turnover in different management systems are strongly mediated by soil mesofauna.  相似文献   

19.
Influence of mineral and symbiotic nitrogen nutrition on proton release of roots, phosphorusuptake and root development of red clover Red clover was cultivated in pots containing a loamy sand soil of low buffer capacity. Nitrogen supply was either NH4NO3 and Ca(NO3)2 in the mineral treatment or Rhizobium fixed N2 in the symbiotic treatment. During six cuts the plants decreased the pH of the soil from 7.6 to 6.0 in the mineral treatment and to 5.2 in the symbiotic treatment. Both treatments yielded the same amount of shoot dry matter per pot. The N2-fixing red clover produced more root fresh weight associated with larger root length, root surface, and root density per pot compared with plants grown with mineral nitrogen. Due to stronger soil acidification and better root growth N2-fixing red clover was able to exploit the rock phosphate component from a partially acidulated P-fertilizer to a higher degree than NH4NO3 or Ca(NO3)2 fed plants. The proton release of symbiotically grown plants could be accounted for by mineral cation excess (difference of cation and anion uptake) in shoots and roots by only 68%. It is therefore assumed that the excess of H+ released was accompanied by a release of anions.  相似文献   

20.
Abstract

Field studies were conducted for four to seven years on two soils, Tangi silt loam (Typic Fragiudalf, fine‐silty, mixed, thermic) and Dexter loam (Ultic Hapludalf, fine‐silty, mixed, thermic), to determine the effects of phosphorus (P) applications on growth and nitrogen (N) content of white clover (Trifolium repens L.) and subterranean clover (Trifolium subterranum L.) and on ammonium (NH4 +)‐ and nitrate (NO3 ‐)‐N, total N, and organic carbon (C) levels in the soils at the end of the study. Phosphorus applications consistently and significantly increased forage yields and led to significantly higher N yields by the clovers. Increases in plant yields and N2‐fixation, however, were not reflected in higher soil N and C levels. On Tangi soil, NH4 +‐ and NO3 ‐‐N levels were lowest where no P was applied but no statistically significant differences (P < 0.05) were found among P rates above 20 kg/ha. On the Dexter soils, no significant differences were found at any P application level. Significant differences due to higher clover yields at increasing P rates were not found in total N or organic C . levels in either soil. Greenhouse evaluations showed no differences in bermuda‐grass yield, N concentration, or total N recovery despite increasing subclover yields in the field during the previous seven years. Harvesting nearly all above ground clover growth caused plant roots to be the major N and C contributor to the soil. It is possible that root production was not increased in proportion to forage production as P applications increased. Perhaps increased microbial activities and some leaching losses also minimized accumulations of N and C released by clover roots.  相似文献   

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