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1.
The present study aimed to elucidate the atmosphere–forest exchange of ammoniacal nitrogen (NHX-N) at a young larch ecosystem. NHX-N exchanges were measured at a remote site in northernmost Japan where 4-year-old larches were growing after a pristine forest had been clear-cut and subsequent dense dwarf bamboo (Sasa) had been strip-cut. The site was a clean area for atmospheric ammonia with mean concentrations of 0.38 and 0.11 μg N m?3 in snowless and snow seasons, respectively. However, there was a general net emission of NHX-N. The annual estimated emission of NHX-N of 4.8 kg N ha?1 year?1 exceeded the annual wet deposition of 2.4 kg N ha?1 year?1, but the weekly exchange fluxes may have been underestimated by 28–60%. The main cause of the ammonia loss from the young larch ecosystem was probably enhanced nitrogen supply stimulated by the cutting of the pristine forest and Sasa, in particular, the Sasa.  相似文献   

2.
Soils stored in stockpiles during opencast mining operations accumulate significant quantities of ammonium (of the order of 200 μg NH4+-N g?1 soil) within the predominantly anaerobic cores of mounds. Upon stockpile dismantling and land restoration, this NH+4-N is rapidly oxidized to NO?3-N, which is readily lost from newly restored soil ecosystems by leaching and denitrification. Experiments were set up to examine how these significant reserves of mineral N might be conserved in such situations. Application of the nitrification inhibitor dicyandiamide was successful in minimizing NO3?-N lost by leaching, though large concentrations of NH4+-N were detected in drainage waters. Straw incorporation decreased nitrate leaching by up to 40%; biomass C was some 40% greater in straw-amended than in unamended soils after 14 weeks, though biomass N was similar in both. Addition of nitrogen-free organic materials (glucose, starch and cellulose) produced different results, with glucose amendment showing the greatest reduction in nitrate leaching in the short term (due to an apparent stimulation of denitrification) whereas addition of cellulose resulted in the most effective conservation of nitrogen over 14 weeks; this was due, at least in part, to uptake of mineral N by the soil microbial biomass.  相似文献   

3.
Sodium chloride, at rates up to 100 mg g?1, was added to a Sassafras sandy loam amended with finely-ground alfalfa to determine the effect of NaCl on CO2 evolution, ammonification, and nitrification in a 14-week study. A NaCl concentration of 0.25 mg g?1 significantly reduced CO2 evolution by 16% in unamended soil and 5% in alfalfa-amended soil. Increasing NaCl progressively reduced CO2 evolution, with no CO2 evolved from the soil receiving 100 mg NaCl g?1. A 0.50 mg NaCl g?1 rate was required before a significant reduction in decomposition of the alfalfa occurred. The NO?2-N + NO?3-N content of the soil was significantly reduced from 40 to 37 μg g?1 at 0 and 0.25 mg NaCl g?1, respectively in the unamended soil. In the alfalfa amended soil, nitrification was significantly reduced at 5 mg NaCl g?1. At 10 mg NaCl g?1, nitrification was completely inhibited, there being only 6 and 2 μg NO?2-N + NO?3-N g?1 in the alfalfa amended and unamended soil, respectively. In the alfalfa amended soil NH+4-N accumulated from 6 μg g?1 at the 0 NaCl rate to a maximum of 54 μg g?1 with 25 mg NaCl g?1. These higher NH+4-N values resulted in a 0.5 unit increase in the pHw over that of the 0 NaCl rate in the alfalfa amended soil. At NaCl concentrations above 25 mg g?1 there was a reduction in NH+4-N. The addition of alfalfa to the soil helped to alleviate the adverse affects of NaCl on CO2 evolution and nitrification.  相似文献   

4.
Sandy loam soil, with added glucose, was incubated anaerobically under N2 and subjected to repeated 1-h C2H2 reduction assays. In the presence of 1% glucose the addition of 50 μg NH4+ ?N/g or of 20 μg NO?3 N/g (untreated soil contained 1.2 μg NH+4?N and 7.10 μg NO?3-N/g) caused at least some suppression of nitrogenase activity. Activity developed when the KCl-extractable soil inorganic nitrogen concentration dropped below 35 μg/g. In the presence of 0.1 or 0.05% glucose the addition of 5 μg NH+4?N/g caused some suppression of nitrogenase activity. However, activity developed when the soil NH4+-N concentration dropped below about 4 μg/g. With 0.1% glucose and 5 μg added NO?2 N/g, activity did not develop until the soil NO?2 -N concentration dropped to zero. Added NO?3 N was rapidly reduced and denitrified to NO?2- N, N2O-N and NH+4 N and furthermore caused some inhibition of CO2 evolution. The data from NH4?-addition experiments are consistent with a nitrogenase repression/ derepression threshold of 4 and 35μg NH+4-N/g at 0.05 and 1% glucose concentrations, respectively. The data from NO?2- and NO?3-addition experiments suggest a combination of repression and toxicity effects in the presence of added NO?3 N.  相似文献   

5.
Abstract. Gross N mineralization and nitrification rates were measured in soils treated with dairy shed effluent (DSE) (i.e. effluent from the dairy milking shed, comprising dung, urine and water) or ammonium fertilizer (NH4Cl) under field conditions, by injecting 15N-solution into intact soil cores. The relationships between gross mineralization rate, microbial biomass C and N and extracellular enzyme activities (protease, deaminase and urease) as affected by the application of DSE and NH4Cl were also determined. During the first 16 days, gross mineralization rate in the DSE treated soil (4.3–6.1 μg N g?1 soil day?1) were significantly (P 14;< 14;0.05) higher than those in the NH4Cl treated soil (2.6–3.4 μg N g?1 soil day?1). The higher mineralization rate was probably due to the presence of readily mineralizable organic substrates in the DSE, accompanied by stimulated microbial and extracellular enzyme activities. The stable organic N compounds in the DSE were slow to mineralize and contributed little to the mineral N pool during the period of the experiment. Nitrification rates during the first 16 days were higher in the NH4Cl treated soil (1.7–1.2 μg N g?1 soil day?1) compared to the DSE treated soil (0.97–1.5 μg N g?1 soil day?1). Soil microbial biomass C and N and extracellular enzyme activities (protease, deaminase and urease) increased after the application of the DSE due to the organic substrates and nutrients applied, but declined with time, probably because of the exhaustion of the readily available substrates. The NH4Cl application did not result in any significant increases in microbial biomass C, protease or urease activities due to the lack of carbonaceous materials in the ammonium fertilizer. However, it did increase microbial biomass N and deaminase activity. Significant positive correlations were found between gross N mineralization rate and soil microbial biomass, protease, deaminase and urease activities. Nitrification rate was significantly correlated to biomass N but not to the microbial biomass C or the enzyme activities. Stepwise regression analysis showed that the variations of gross N mineralization rate was best described by the microbial biomass C and N.  相似文献   

6.
 The 15N isotope dilution method was combined with a field incubation technique to provide simultaneous measurements of gross and net rates of N turnover in three long-term swards: unfertilized (Z) or receiving N either from N fixation as clover (C), or as 200 kg fertilizer N ha–1 year–1 (F). Uniform N enrichment of soil microplots was achieved with a multi-point soil injector to measure mineralization/immobilization turnover and nitrification over a 4-day incubation. Net rates of mineralization ranged between 0.6 and 2.9 μg N g–1 day–1 and in all three treatments were approximately half the gross rates. Nitrification rates (gross) were between 1.0 and 1.6 μg N g–1 day–1. In the F treatment, the turnover of NH4 +-N and NO3 -N pools was on a 2- and 4-day cycle, respectively, whereas in the N-limited treatments (C and Z) turnover rates were faster, with the NO3 -N pools turning over twice as fast as the NH4 +-N pools. Therefore, available N was recycled more efficiently in the C and Z treatments, whereas in the F treatment a higher N pool size was maintained which would be more vulnerable to leakage. A large proportion of the added 15N was recovered in the soil microbial biomass (SMB), which represented a 4–5 times larger sink for N than the plant biomass. Although the C treatment had a significantly lower SMB than the grass-only treatments, there were no differences in microbial activity. Gross rates of nitrification increased along the gradient of N input intensity (i.e. Z<C<F), and the addition of a nitrification inhibitor (C2H2) tended to increase microbial immobilization, but did not influence plant N uptake. In this study, the value of combining different techniques to verify net rates was demonstrated and the improved methodology for 15N labelling of soil enabled measurements to be obtained from relatively undisturbed soil under natural field conditions. Received: 25 May 1999  相似文献   

7.
Abstract

To determine the relationships between microbial biomass nitrogen (N), nitrate–nitrogen leaching (NO3-N leaching) and N uptake by plants, a field experiment and a soil column experiment were conducted. In the field experiment, microbial biomass N, 0.5 mol L?1 K2SO4 extractable N (extractable N), NO3-N leaching and N uptake by corn were monitored in sawdust compost (SDC: 20 Mg ha?1 containing 158 kg N ha?1 of total N [approximately 50% is easily decomposable organic N]), chemical fertilizer (CF) and no fertilizer (NF) treatments from May 2000 to September 2002. In the soil column experiment, microbial biomass N, extractable N and NO3-N leaching were monitored in soil treated with SDC (20 Mg ha?1) + rice straw (RS) at five different application rates (0, 2.5, 5, 7.5 and 10 Mg ha?1 containing 0, 15, 29, 44 and 59 kg N ha?1) and in soil treated with CF in 2001. Nitrogen was applied as (NH4)2SO4 at rates of 220 kg N ha?1 for SDC and SDC + RS treatments and at a rate of 300 kg N ha?1 for the CF treatment in both experiments. In the field experiment, microbial biomass N in the SDC treatment increased to 147 kg N ha?1 at 7 days after treatment (DAT) and was maintained at 60–70 kg N ha?1 after 30 days. Conversely, microbial biomass N in the CF treatment did not increase significantly. Extractable N in the surface soil increased immediately after treatment, but was found at lower levels in the SDC treatment compared to the CF treatment until 7 DAT. A small amount of NO3-N leaching was observed until 21 DAT and increased markedly from 27 to 42 DAT in the SDC and CF treatments. Cumulative NO3-N leaching in the CF treatment was 146 kg N ha?1, which was equal to half of the applied N, but only 53 kg N ha?1 in the SDC treatment. In contrast, there was no significant difference between N uptake by corn in the SDC and CF treatments. In the soil column experiment, microbial biomass N in the SDC + RS treatment at 7 DAT increased with increased RS application. Conversely, extractable N at 7 DAT and cumulative NO3-N leaching until 42 DAT decreased with increased RS application. In both experiments, microbial biomass N was negatively correlated with extractable N at 7 DAT and cumulative NO3-N leaching until 42 DAT, and extractable N was positively correlated with cumulative NO3-N leaching. We concluded that microbial biomass N formation in the surface soil decreased extractable N and, consequently, contributed to decreasing NO3-N leaching without impacting negatively on N uptake by plants.  相似文献   

8.
Immobilization of N was measured in a fumigated and in an unfumigated soil by adding (15NH4)2SO4 and following the disappearance of inorganic label from the soil solution and its simultaneous conversion to soil organic N. Calculations based on the measurement of organically-bound 15N gave more consistent values for immobilization than did calculations based on the measurement of the disappearance of label from solution. The fumigated soil immobilized 6.6 μg N g?1 N g?1 soil in 10 days at 25°C, the unfumigated control 4.8 μg. The corresponding gross mineralization rates were 34.9 and 5.6 μg N g?1 soil in 10 days.Addition of 58 μg N as (15NH4)2SO4 to the fumigated soil increased the quantity of the ynlabelled NH4-N extracted at the end of 10 days from 33.8 to 37.8 μg Ng?1 soil, i.e. there was a positive Added Nitrogen Interaction (ANI). The added labelled N produced this ANI, not by increasing the rate of mineralization of organic N, but by standing proxy for unlabelled N that otherwise would have been immobilized.A procedure for calculating biomass N from the size of the flush of mineral N caused by fumigation is proposed. Biomass N (BN) is calculated from the relationship BN = F'N/0.68 where F'N is [(N in fumigated soil incubated for 10 days — (N in unfumigated soil incubated for 10 days)].  相似文献   

9.
14C-labelled glucose and 15N-labelled KNO3 were added to soil and the microbial biomass during 42 days' incubation was estimated using the chloroform fumigation-incubation method (CFIM). By day 1, most of the glucose (1577 μgCg?1 soil) was metabolized and 110 μg NO?3-Ng?1 soil were immobilized. In situ values for the proportions of biomass C (kC) and biomass N (kN) mineralized during the 10 days after CHCl3 fumigation were determined on the basis that the immobilized labelled C and N remaining in the soil at this time were present as living microbial cells and their associated metabolites. The tracer data indicated that biomass C could be calculated by applying a kc value of 0.41 to the CO2-C evolved from the fumigated sample without subtraction of an unfumigated “control”. Biomass N was estimated from the net NH4?-N accumulation during the fumigation-incubation. The problem of reimmobilization of NH+4-N where organisms of wide C:N ratio occur was overcome by adjusting the value of kN according to the ratio of CO2-C evolved: net NH4+-N accumulated during the fumigation-incubation (CF:NF).A CF:NF ratio of 6:1 resulted in a kN of 0.30 whereas a ratio of 13:1 indicated a kN of 0.20.  相似文献   

10.
Abstract

Rice variety IR 36, grown under flooding, was studied in 1998 to determine the effects of fly ash, organic, and inorganic fertilizers on changes in pH and organic carbon, release of nutrients (NH4 +-N, Bray's P, and NH4OAc K), and dehydrogenase activity in an acid lateritic soil at 15-day intervals. Application of fly ash at 10?t?ha?1 alone did not improve the availability of NH4 +-N, or P, as well as the rice grain yield. Availability of NH4 +-N (35.3–36.9?mg?kg?1), and P (12.3–14.6?mg?kg?1) at 15 days after transplanting, and rice grain yields (48.0–51.7?g per pot) were similar under the various fertilization sources such as inorganic fertilizer alone, inorganic fertilizer?+?fly ash or inorganic fertilizer?+?green manure?+?fly ash. Mean dehydrogenase activity was the highest (8.47?µg triphenyl formazon g?1 24?h?1) under the mixed fertilization treatments with green manure. At the end of the cropping season (75 days after transplanting), pH, organic carbon, and dehydrogenase activity were higher under the mixed fertilization treatments involving green manure by 3, 15 and 154%, respectively, compared with the inorganic fertilizer alone.  相似文献   

11.
ABSTRACT

Understanding how plants use of various nitrogen (N) sources is important for improving plant N use efficiency in organic farming systems. This study investigated the effects of farming management practices (organic and conventional) on pakchoi short-term uptake of glycine (Gly), nitrate (NO3 ?) and ammonium (NH4 +) under two N level conditions. Results showed that plant N uptake rates and N contributions from the three N forms in the low N (0.15 μg N g?1 dry soil) treatment did not significantly differ between the organic and conventional soils, except the significantly greater Gly contribution in organic soil at 24 h after tracer addition. Under high N (15 μg N g?1 dry soil) conditions, the N uptake rates, uptake efficiencies, and N contributions of Gly and NH4 +-N were significantly greater in pakchoi cultivated in the organic soil compared to conventional soil, whereas the N uptake rates and N contributions from NO3 -N decreased in pakchoi cultivated in the organic soil. The greater Gly-N uptake in plants grown in high-N treated organic soil may be related to the greater gross N transformation, Gly turnover rate and the increased expression of an amino acid transporter gene BcLHT1. Intact Gly contributed at most 6% to Gly-derived N at 24 h after tracer additions, which accounting for about 1.24% of the total N uptake in organic soil. Our study suggested that Gly-N and other organic source N might serve as a more important compensatory N source for plants in organic farming.  相似文献   

12.
15N studies were conducted using hydroponically grown tea (Camellia sinensis L.) plants to clarify the characteristics of uptake, transport and assimilation of nitrate and ammonium. From the culture solution containing 50 mg L-1 N03-N and 50 mg L-1 NH.-N, the uptake of NH3-N after 24 h was twice as high as that of NO3-N, while the uptake of N03-N from the culture solution containing 90 mg N03-N and 10 mg NH3-N was twice that of NH4-N. The presence of 0.4 mM Al had no significant effect on the N03-N and NH4-N uptake from the culture solutions containing 50 mg L-1 N03-N and 50 mg L-1 NH4-N, 90 mg L-1 N03-N and 10 mg L-1 NH4-N or 99 mg L-1 N03-N and 1 mg L-1 NH4-N. Transport of N03-derived N to young leaves was much more rapid than that of NH4-derived NO3 and NH4-derived N was largely retained in the roots and lower stem. Young and mature shoots separated from the roots absorbed more N03-N than intact plants. Nitrate assimilation occurred in both, roots and young as well as mature leaves. Internal cycling of N03-derived Nand NH4-derived N from one root part to another part was not appreciable after 28 h, suggesting that a longer of time is required for cycling in woody plants.  相似文献   

13.
Increases in N deposition (wet and dry) have been associated with a decline in semi-natural plant communities, adapted for growth on nutrient poor soils in the UK and Europe. The impacts of N deposition applied as either wet NH4 + or gaseous NH3 on vegetation (7 species) from acid moorland in SE Scotland were compared in a dose-response study. Wet N deposition at 0, 8, 16, 32, 64, 128 kg N ha?1 y?1 was applied as NH4Cl, and dry deposition as gaseous NH3 (2, 6, 20, 50, 90 µg NH3 m?3) under controlled conditions in open-top chambers. A strong linear dose-response relationship (p<0.05) was found between foliar N content in all seven plant species and applied NH4?N. However, in the NH3 treatment, only C. vulgaris and P. commune showed a significant response to increasing N additions. NH3 was found to increase the rate of water loss in Calluna in both autumn and winter by comparison with wet deposition. For Eriophorum vaginatum, the NH3 and NH4 + treatments showed significant N dose response relationships for biomass. A significant increase in above ground biomass, proportional to the added N, was found for Narthecium ossifragum when N was applied as NH3 compared to NH4 +.  相似文献   

14.
Nitrogen mineralization and immobilization were investigated in two soils incubated with ammonium sulphate or pig slurry over a range of temperatures and moisture contents. A reduction in the mineralization of soil organic N was observed in soils incubated with 100 μg NH4+-Ng?1 soil as ammonium sulphate at 30°C but not at lower temperatures. Addition of 100 μg NH4+-N g?1 soil as pig slurry resulted in a period of nett immobilization lasting up to 30 days at 5°C. Although the length of the immobilization phase was shorter at higher temperatures the total N immobilized was similar. The subsequent rate of mineralization in slurry-treated soils was not significantly greater (P = 0.05) than in untreated soils. There was no evidence of any subsequent increased mineralization arising from the immobilized N or slurry organic N for up to 175 days. The rate of immobilization was found to increase with increasing moisture content, though the period of nett immobilization was shorter, so that the amount of N immobilized was similar over a range of moisture contents from 10 to 40%. Approximately 40% of the NH4+-N in the slurry was immobilized under the incubation conditions used.  相似文献   

15.

Purpose

The nitrification inhibitor dicyandiamide (DCD) has been shown to be highly effective in reducing nitrate (NO3 ?) leaching and nitrous oxide (N2O) emissions when used to treat grazed pasture soils. However, there have been few studies on the possible effects of long-term DCD use on other soil enzyme activities or the abundance of the general soil microbial communities. The objective of this study was to determine possible effects of long-term DCD use on key soil enzyme activities involved in the nitrogen (N) cycle and the abundance of bacteria and archaea in grazed pasture soils.

Materials and methods

Three field sites used for this study had been treated with DCD for 7 years in field plot experiments. The three pasture soils from three different regions across New Zealand were Pukemutu silt loam in Southland in the southern South Island, Horotiu silt loam in the Waikato in the central North Island and Templeton silt loam in Canterbury in the central South Island. Control and DCD-treated plots were sampled to analyse soil pH, microbial biomass C and N, protease and deaminase activity, and the abundance of bacteria and archaea.

Results and discussion

The three soils varied significantly in the microbial biomass C (858 to 542 μg C g?1 soil) and biomass N (63 to 28 μg N g?1), protease (361 to 694 μg tyrosine g?1 soil h?1) and deaminase (4.3 to 5.6 μg NH4 + g?1 soil h?1) activity, and bacteria (bacterial 16S rRNA gene copy number: 1.64?×?109 to 2.77?×?109 g?1 soil) and archaea (archaeal 16S rRNA gene copy number: 2.67?×?107 to 3.01?×?108 g?1 soil) abundance. However, 7 years of DCD use did not significantly affect these microbial population abundance and enzymatic activities. Soil pH values were also not significantly affected by the long-term DCD use.

Conclusions

These results support the hypothesis that DCD is a specific enzyme inhibitor for ammonia oxidation and does not affect other non-target microbial and enzyme activities. The DCD nitrification inhibitor technology, therefore, appears to be an effective mitigation technology for nitrate leaching and nitrous oxide emissions in grazed pasture soils with no adverse impacts on the abundance of bacteria and archaea and key enzyme activities.  相似文献   

16.
Terrestrial ecosystems are predicted to experience an increasing level of atmospheric nitrogen (N) deposition, which may cause significant shifts in plant community composition and concomitantly stimulate soil acidification. However, little is known concerning the effects of N deposition on belowground microbial communities in alpine grassland ecosystems such as on the Tibetan Plateau. This study examined the responses of soil N-transforming microbes (measured after DNA extraction and quantitative PCR), soil microbial biomass C (SMBC) and N (SMBN), and soil enzyme activities to different forms (NH4 +-N, NO3 ?-N, and NH4NO3-N) and rates (1.5 and 7.5 g N m?2 year?1, denoted as low and high N, respectively) of N fertilization (addition) in two successive plant growing seasons. The N rate, not N form, influenced the abundance of ammonia-oxidizing archaea (AOA). High N addition significantly increased ammonia-oxidizing bacteria (AOB) abundance which differed across different N form treatments. Nitrogen addition had no significant impact on the abundance of soil denitrifiers. The SMBC and SMBN were significantly decreased by high N additions, but no difference was found among different N forms. Despite higher urease activities being detected in the late plant growing season, the activities of invertase and alkaline phosphomonoesterase stayed unchanged irrespective of the different N amendments and plant growing season. Significant positive correlations were found between potential nitrification rates and AOB abundances. These results highlight that AOB seemed to respond more sensitively to different N fertilization and might have prominent roles in soil N cycling processes in this Tibetan Plateau alpine meadow than AOA.  相似文献   

17.
The effects of temperature, moisture content and the addition of pig slurry on nitrification in two soils were studed. There was no accumulation of NO2?-N under the incubation conditions investigated and the accumulation of NO3?-N was linear for additions of 50–250 μg NH4+-N g? soil, either as ammonium sulphate or as pig slurry. Nitrate formation was treated as a single step, zero order process to enable a rate constant to be calculated. Nitrification rate increased with increasing moisture content up to the highest level tested, soil water potential ?8.0 kPa, corresponding to approximately 60% of water holding capacity in both soils. Measurable nitrification was found in both soils at the lowest moisture content (soil water potential ?1.5 MPa) and temperature (5° C) tested. The nitrification rate constant in soils treated with 50 μg NH4+-N g? soil was not significantly affected (P = 0.05) by the form of ammonium added. Addition of 250 μg NH4+-N as ammonium sulphate caused a marked inhibition of nitrification at all moisture contents and temperatures. Addition of 250 μg NH4+-N as pig slurry caused a marked increase in nitrification rate, the increase being greater at the higher temperatures and moisture contents.  相似文献   

18.
The incidence and severity of take-all disease, due to Gaeumannomyces graminis (Sacc.) Arx & Olivier var. tritici Walker, was observed on spring barley plants growing in soil in two glasshouse experiments. Soil amendments of NH+4-N significantly increased the number of diseased plants and roots during the first month after germination in comparison with controls unamended with N (P < 0.05). No significant difference in the incidence of take-all disease was detected between more mature barley plants growing in soil amended with either NH+4 or NO?3-N and unamended controls. The least take-all disease in 3 month-old barley plants was observed when N was supplied as foliar sprays of urea at 0.5 mg N kg?1 soil (P < 0.01). There was no significant correlation between the degree of infection and the NH+4-N to NO?3-N ratio in the rhizosphere soil  相似文献   

19.
Biodegradation rates of oily waste in soil can be limited by mineral nutrients, particularly N and P. A laboratory incubation experiment was carried out to investigate the influence of N forms, nitrate (NO? 3-N) vs ammonium nitrogen (NH+ 4-N), and sources, i.e., the conjugate cations/anions, on C mineralization rate (CMR) was determined daily by measuring the CO2 evolved using gas chromatography. The CMR and the cumulative C mineralized (CCM) varied with the form and/or the source of N applied. The greatest enhancement in CMR occurred in the NO? 3-treatments in which the source conjugate cation was Ca+2. The addition of P fertilizer further enhanced C mineralization rates irrespective of the form and/or the source of N added. The results show that up to 45% of the added oily waste mineralized as CO2-C in 28 d. The residual P and N (NO? 3-N plus NH+ 4-N) data showed that approximately 90% of the added P and N were utilized for oil decomposition. The amount of residual NO? 3-N appeared to have an inverse relationship with CCM. The NO? 3-N utilization occurred at the expense of NH+ 4-N and this was particularly high in the treatments which received P.  相似文献   

20.
To-date, assessments of nitrogen use efficiency (NUE) of sugarcane have not included the contribution of its components, nitrogen uptake efficiency (NUpE) and nitrogen utilization efficiency (NUtE). This study determined these values, based on biomass and plant nitrogen (N) content, in two four-month-old pot-grown genotypes. The treatments included six N regimes, with nitrate (NO3N) or ammonium (NH4+-N) supplied alone, or as NO3?-N for the first 6 weeks and then NH4+-N until harvest, each as 4 or 20 mM. Regardless of the N form, NUE was higher at four than at 20 mM due to significantly higher NUpE at low N supply. The results indicated that there was luxury N uptake and preference for NH4+-N nutrition, which resulted in the highest determined NUE. There were significant differences between genotypes in biomass, morphological growth parameters, N uptake, total plant N and NUE, the latter matching previously established sucrose yield-based NUE field rankings.  相似文献   

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