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美洲黑杨基因资源收存及其遗传评价的研究 总被引：14，自引：0，他引：14
从１７个国家引进３３１个黑杨派无性系（其中５２个美洲黑杨无性系）,在山东省长清县营建我国第一个黑杨派无性系基因库。连续１０年对基因库内美洲黑杨无性系进行了多性状系统研究,结果表明各无性系间在物候期、生长、生根、抗寒和抗病虫等方面均存在着显著的差异,遗传变异丰富;系间的主要性状与无性系起源纬度及各性状间存在一定相关性。主要材料性状变异的研究结果表明,３８个８年生美洲黑杨无性系在木材基本密度、纤维长度无性系间变异达到极显著水平;木材密度和纤维长度与树高和胸径呈一定相关性;木材密度和纤维长度这两个性状在遗传上相互独立,受不同遗传机制控制。利用分子遗传标记ＲＡＰＤ技术研究库内美洲黑杨无性系ＤＮＡ多态性,结果表明本库美洲黑杨ＤＮＡ多态率为８６％,再次证明本库美洲黑杨遗传多样性高,可为我国杨树改良提供丰富的育种材料。 相似文献
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我国美洲黑杨无性系引种研究现状 总被引：2，自引：0，他引：2
正目前,全世界黑杨派有2个种,即美洲黑杨(Populus deltoides)和欧洲黑杨(Populus nigra),而我国只有新疆阿尔泰地区有欧洲黑杨分布,美洲黑杨却没有天然分布[1]。自1950年意大利选育出几个美洲黑杨栽培无性系以来,美洲黑杨及其栽培无性系引起了人们的重视。目前世界各国先后培育出了美洲黑杨和欧洲黑杨的一系列杂交种,主要有美洲黑杨和欧洲黑杨的杂种后代———欧美 相似文献
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反义磷脂酶Dγ基因与几丁质酶基因转化美洲黑杨G2 总被引：4，自引：1，他引：4
建立美洲黑杨G2组培再生体系,确立美洲黑杨G2分化最适宜培养基和生根培养基.然后分别进行卡那霉素和潮霉素敏感性试验,利用叶盘法依次将反义磷脂酶Dγ(Anti-PLDγ)基因和几丁质酶(CH5B)基因转入美洲黑杨G2中.首先转入反义磷脂酶Dγ基因,经诱导不定芽及生根阶段卡那霉素(选择性抗生素)连续筛选,获得了21株卡那霉素抗性植株.抗性植株经PCR及PCR-Southern杂交检测,有13株均呈阳性,证明Anti-PLDγ基因成功整合到美洲黑杨G2基因组中.耐盐性试验表明,4株转基因植株抗NaCl能力比对照都有不同程度提高.选择4号株系继续转入几丁质酶基因,通过潮霉素(选择性抗生素)筛选不定芽及诱导生根获6株潮霉素抗性植株,经PCR及PCR-Southern杂交检测,6株均呈阳性,证明CH5B基因成功整合到美洲黑杨G2基因组中.由于CH5B基因与报告基因GUS处于同一开放阅读框(ORF)内,因此通过GUS基因的组织化学染色分析,证明了6株转化植株几丁质酶基因均获得正常表达. 相似文献
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mtlD/gutD双价基因转化美洲黑杨×青杨的研究 总被引：14，自引：0，他引：14
利用叶盘法开展了mtlD gutD双价基因转化美洲黑杨×青杨的研究 ,经诱导不定芽及诱导生根阶段卡那霉素 (选择性抗生素 )连续筛选 ,获得了 38株卡那霉素抗性植株。抗性植株经PCR检测 ,2 8株呈阳性。对其中 5株PCR阳性植株进行Southern及Western杂交 ,有 4株二者均呈阳性 ,证明双价基因成功整合到美洲黑杨×青杨基因组中并得到表达。耐盐实验表明这 4株转基因植株抗NaCl能力比对照均有不同程度提高 相似文献
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美洲黑杨(Populus deltoides Marsh.)是北美的一个重要杨树种,69杨(P.deltoid-es CV.‘I-69/55’或者 P.deltoides CV.‘L-ux’是意大利从美洲黑杨中选育出的一个优良无性系。各国杨树育种工作者用美洲黑杨与欧洲黑杨杂交培育出许多欧美杨品种,也用美洲黑杨与青杨派树种杂交培育出不少品种。近年来,一些国家对美洲黑杨与青杨派树种的杂交尤为重视,如比利时、新西兰等国用美洲黑杨与毛果杨(P.tricoca-pa)、滇杨(P.yunnanensis)等杂交,选出了一些较好的无性系。我国青杨派树种的种类多,分布广。育种工作者曾用小叶杨(P.simonii)、青杨(P.cathayana)等与欧洲黑杨(P.nigra) 相似文献
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美洲黑杨无性系的生长与抗寒性 总被引：2，自引：0，他引：2
美洲黑杨无性系在我国北方栽培往往出现冻害，而影响生长。作者对黑杨基因库中的２１个美洲黑杨无性系的生长与抗寒性的遗传异规律进行了研究，结果表明：二者在无性系间存在极显著差异，相互间呈负相关关系。 相似文献
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本文对引进黑杨派黑杨树基因库内无性系主要病虫发生情况进行了调查研究，结果表明：在杨扇舟蛾、杨树水泡溃疡病和破腹病等３种发生病虫中，以破腹病危害最为严重；综合发病情况以欧美杨较美洲黑杨为重；就破腹病而言，其抗病力美洲黑杨无性系较多地受遗控制，欧美杨则受环境影响较大，美洲黑杨的抗病能力与无性系的抗寒性有显著的正相关关系，通过聚类分析可分别把美洲黑杨、欧美杨无性系分为高感、中感、中抗和高抗等４类。文中还 相似文献
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QINGuanq-hua JIANGYue-zhong QIAOYu-ling B.Nottola 《林业研究》2003,14(3):225-229
Poplar is one of the dominant tree species for the establishment of fast growing plantations in Shandong Province.Eighteen poplar clones belonging to Populus aigeiros section were introduced from Italy, Turkey and domestic regions. Populus deltoides cv. ‘Lux‘ 1-69/55 (1-69), which was widely used in Shandong Province, China, was taken as control clone (1-69).Following a randomized complete block design, seedling test and controlled afforestation trials were carried out at Juxian County,Caoxian County and Laiyang City. The results showed that the poplar clone (Populus x euramericana cv. ‘102/74‘), namely 102/74, performed well both in terms of adaptability and growth rate. The mean height of 13.9 m (H), diameter at breast height of 18.0 cm (DBH) and volume growth of 0.1445 m3 (V) were 2.2 %, 21.6% and 52.9% higher than those of 1-69 (CK), respectively,at the age of 5 years at three experimental sites. Moreover, the clone can be propagated easily and showed high resistance to poplar disease, pest as well as salinity and had longer growing period. Furthermore, wood basic density and fiber length of new poplar clone (102/74) were as same as 1-69 (CK). It was concluded that the selected clone (102/74) was ideal for the estab-lishment of fast-growing poplar plantations, especially for the pulpwood plantations in Shandong Province. 相似文献
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为了检验已培育出的适于我国华北地区生长的一批欧美杨新品种(以鲁克斯杨P.delt-oides cv‘Lux’(ex.I-69/55)简称69杨为母本,欧洲黑杨及其变种为父本)的抗寒性,进行了2年(1988～1989年)的抗寒实验。实验用自然低温冷冻、人工低温冷冻、苗木失水试验和冷冻后生长恢复试验四种方法,对欧美杨新品种及对照I-69杨和I-214杨的抗寒性进行了研究,结果是欧美杨新品种抗寒性极显著地高于母本I-69杨,接近或稍高于我国黄河流域广为栽培的I-214杨,遗传了父本欧洲黑杨的抗寒能力。实验为欧美杨新品种的栽培范围提供了可靠的科学依据。 相似文献
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QinGuanghua JiangYuezhong B.Nottola 《中国林学(英文版)》2003,5(3):21-27
18 clones of Populus deltoides and P. x euramericana were introduced from Italy, Turkey and domestic in 1986 and 1987 with the agreement of FAO. A completely random block design was applied and the seedling test and controlled afforestation trials had been held at Jinan City, Gaotang County and Juxian County, Shandong Province respectively. The results showed that two clones, namely S307-26 (P. deltoides cv.‘S307-26‘) and PE-19-66 (P. deltoides cv.‘PE-19-66’) performed well both in terms of adaptability and growth characteristics. The mean growth volumes (V) of single tree of S307-26 and PE-19-66 were 0.181 2 m3 and 0.164 9 m^3, which were 42.0 % and 29.2 % larger than that of I-69 (P. deltoides cv.‘Lux‘ 1-69/55 ) (CK), respectively, at the age of 5years at three experimental sites. Results of variance analyses and T test (LSD) for variables showed that above mentioned two clones were significantly different with 1-69 (CK). Further analyses found that both clones could be propagated easily and showed high resistance to poplar diseases, pests, drought, cold as well as strong wind, and had long growing period. Furthermore, mechanical properties were also better and fiber length was longer than that of 1-69 (CK). It was concluded that both S307-26 and PE-19-66 were ideal varieties for the establishment of fast growing poplar plantations, especially of the wood pulp forest plantations in the region. 相似文献
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Qin Guanghua Jiang Yuezhong Qiao Yuling Shandong Academy of Forestry Jinan P.R.China 《中国林学(英文版)》2003,5(4)
1IntroductionAdversitystudyisoneofthefocusesinplantphysiology.However,thepriorityhadbeenputontheselectionofpoplarcloneswithfastgrowthrateandbetterwoodqualityonpoplarbreedinginthepastfewdecades.Therewerelimitedresearchestargetedontheresistancebreeding.Moreover,mostoftheresearchonadversityresistancewasconductedonPopulustomentosa(Peietal.1994,Duanetal.1998)andPopulusussuriensis(Jiangetal.1999).LittlewasknownabouttheadversityresistanceofpoplarclonesinSectionAigeiros(Wang1982,Zhu1990,Liuetal.1… 相似文献
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1995年对伊犁州19个引种杨树主栽品种进行扦插育苗试验,通过对1年生苗木地径、苗高随机抽样调查后进行方差及聚类分析,结果表明:伊犁州19个杨树引种品种的苗高、地径存在极显著差异(P<0.01),1年生扦插苗苗高及地径最大差距可达1.04 m、0.83 cm。其中,生长最快的品种有78-8、优胜003、格氏、中林23等4个,其苗高平均为3.22 m,地径平均为2.36 cm;生长较快的有78-133、中林、沙兰杨等12个品种;表现最差的是斯大林、加小×俄9#、健227等3个品种。 相似文献
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据不完全统计,杨树人工林全国共有114.8万ha,为能进一步提高杨树经营管理水平,编制杨树二元立木材积表已成为当务之急。作者利用从1981年起经过5a时间测定与收集16个树种共6383株实测样木资料,编制了12个杨树二元立木材积表,供生产、科研工作应用。 相似文献